Pulse Polarization Research Articles

Influence of Voltage, Pulselength and Presence of a Reverse Polarized Pulse on an Argon–Gold Plasma during a High-Power Impulse Magnetron Sputtering Process

This work aims to provide information about the deposition of gold via bipolar high-power impulse magnetron sputtering (HIPIMS) in order to identify suitable process parameters. The influences of voltage, pulse length and the kick-pulse on an argon–gold plasma during a bipolar high-power impulse magnetron sputtering deposition process were analysed via optical emission spectroscopy (OES) and oscilloscope. The voltage was varied between 700 V and 1000 V, the pulse length was varied between 20 µs and 100 µs and the process was observed once with kick-pulse and once without. The influence of the voltage on the plasma was more pronounced than the influence of the pulse width. While the intensity of several Au I lines increased up to 13-fold with increasing voltages, only a less-than linear increase in Au I brightness with time could be identified for changes in pulse length. The intensity of excited argon is only minimally affected by changes in voltages, but follows the evolution of the discharge current, with increasing pulse lengths. Contrary to the excited argon, the intensity emitted by ionized argon grows nearly linearly with voltage and pulse length. The reverse polarised pulse mainly affects the excited argon atoms in the plasma, while the influence on the ionized argon is less pronounced, as can be seen in the the spectra. Unlike the excited argon atoms, the excited gold atoms appear to be completely unaffected by the kick-pulse. No ionization of gold was observed. During the pulse, a strong rarefaction of plasma takes place. Very short pulses of less than 50 µs and high voltages of about 1000 V are to be preferred for the deposition of gold layers. This paper offers a comprehensive overview of the gold spectrum during a HIPIMS process and makes use of optical emission spectroscopy as a simple measuring approach for evaluation of the reverse polarized pulse during a bipolar process. Future uses of the process may include the metallization of polymers.

Single pulse polarization study of pulsars B0950 + 08 and B1642 − 03: micropulse properties and mixing of orthogonal modes

ABSTRACT We present the results of a high-time resolution polarization study of single pulses from pulsars B0950 + 08 and B1642 − 03. Single pulses from pulsar B0950 + 08 sometimes show isolated micropulses without any significant associated subpulse emission. Assuming that the properties of such micropulses represent the intrinsic nature of micropulse emission, we characterize the width and polarization properties of these ‘intrinsic’ microstructures. Most of the ‘intrinsic’ micropulses ($\sim 90~{{\ \rm per\ cent}}$) follow common characteristic polarization properties, while the average width of these micropulses is consistent with the general micropulse population from this pulsar. Single pulses from these pulsars show a diverse range of polarization properties, including depolarization and mixing of two orthogonal modes resulting in polarization position angle jumps. We present a superposition model of the two orthogonal modes which can explain depolarization, the observed position angle jumps, and associated changes in other polarization parameters.

Pair production in an electron collision with a radially polarized laser pulse

Using 3D numerical simulations, we show that in the collision of an electron with a counter-propagating laser beam, the radial polarization of the laser pulse leads to more efficient electron–positron pair production via multiphoton Breit–Wheeler process compared to the linearly polarized one having the same energy, duration, and amplitude. The reason is that in the case of the radially (or azimuthally) polarized laser pulse, the area of a strong field region is greater than that in the case of a linear one. As a consequence, more electrons experience a strong field region with sufficient energy, that, in turn, results in a higher number of created electron–positron pairs.

Polypyrrole/MoO3 composite as an appropriate anode of microbial fuel cell in both pulsed and permanent polarization with excellent performance

The surface of the graphite sheet is electrochemically modified with MoO3/polypyrrole composite (MoO3/PPy/Gr) to improve the performance of the anode of the microbial fuel cell (MFC) via strengthening the growth of the biofilm and increasing connection between the biofilm and the anode surface. Surface characterization of modified bioanode is investigated by Attenuated total reflection (ATR), The Energy Dispersive X-ray (EDX), and Field emission scanning electron microscopy (FE-SEM) analysis, where electrochemical evaluations are investigated with various tests such as various test circuits (VCR) test, chronopotentiometry, chronoamperometry, Electrochemical Impedance Spectroscopy (EIS). The MoO3/PPy/Gr bioanode, compared to PPy/Gr and MoO3/Gr bioanodes shows excellent performance given the capacitive behavior, generation of transient and permanent currents, and momentary intensity of the oxidation of organic materials. MFC equipped with MoO3/PPy/Gr bioanode shows a maximum power density of 380 mW/m2 in permanently current mode, 1.65 and 3.3 times the power density obtained using PPy/Gr and Gr bioanodes, respectively. Chronopotentiometry evaluations display that the specific capacitance for MoO3/PPy/Gr bioanode is 1454 mF/cm2, more than PPy/Gr and Gr (1000 and 695 mF/cm2, respectively).

Nanoscale reshaping of resonant dielectric microstructures by light-driven explosions

Femtosecond-laser-assisted material restructuring employs extreme optical intensities to localize the ablation regions. To overcome the minimum feature size limit set by the wave nature of photons, there is a need for new approaches to tailored material processing at the nanoscale. Here, we report the formation of deeply-subwavelength features in silicon, enabled by localized laser-induced phase explosions in prefabricated silicon resonators. Using short trains of mid-infrared laser pulses, we demonstrate the controllable formation of high aspect ratio (>10:1) nanotrenches as narrow as {sim }{{{{{boldsymbol{lambda }}}}}}{{{{{boldsymbol{/}}}}}}{{{{{boldsymbol{80}}}}}}. The trench geometry is shown to be scalable with wavelength, and controlled by multiple parameters of the laser pulse train, such as the intensity and polarization of each laser pulse and their total number. Particle-in-cell simulations reveal localized heating of silicon beyond its boiling point and suggest its subsequent phase explosion on the nanoscale commensurate with the experimental data. The observed femtosecond-laser assisted nanostructuring of engineered microstructures (FLANEM) expands the nanofabrication toolbox and opens exciting opportunities for high-throughput optical methods of nanoscale structuring of solid materials.

Multiplex Quantification of Exosomes via Multiple Types of Nanobeads Labeling Combined with Laser Scanning Detection.

In recent years, exosomes have attracted attention in many aspects from basic research to clinical application, including therapeutic reagents or biomarkers for liquid biopsy. The increasing understanding of exosome's heterogeneous properties is expected to lead to more advanced exosome research, and there is therefore a need for a multiplex system that can easily classify and analyze exosomes in complex biological samples according to their properties. In this study, we developed a simple and sensitive multiplexed exosome quantification system based on ExoCounter, an exosome quantification system utilizing optical disk technology, by introducing nanobeads made of different materials as exosome labeling substances. The refractive indices suitable for nanobead materials were analyzed by computer simulation of optical diffraction generated by nanobeads. The results showed that polymer (FG), Au, and Ag nanobeads exhibited superior discrimination capability in terms of the amplitude and polarity of detection pulses generated by each nanobead. The specificity and detection sensitivity of three types of nanobeads were confirmed by detecting HER2-positive exosomes with anti-HER2 antibody-conjugated nanobeads. Furthermore, CD147-positive, HER2-positive, and CD81-positive exosomes in 12.5 μL of serum were simultaneously quantified with high discrimination performance using the anti-CD147 antibody, anti-HER2 antibody, or anti-CD81 antibody conjugated for FG beads, Au nanobeads, or Ag nanobeads, respectively. A limit of detection was also evaluated as low as 210 exosomes/μL. This system is a promising tool for advanced exosome research because it enables multiplexed detection of heterogeneous exosomes in serum with high specificity, accuracy, and sensitivity without purification.

The topology and polarization of subbeams associated with the drifting subpulse emission of pulsar B0943+10 – VII. Analysis of transitional intervals in the mode-switching process

ABSTRACT We report a study of the transitional intervals between pulsar B0943+10’s two primary Q and B emission modes using Arecibo 327-MHz observations. The goal of this study was to detect signs of a ‘transitional’ mode at 327 MHz, discovered recently at lower frequencies. We have found subpulse drift and profile form patterns at 327 MHz similar to those identified at lower frequencies in the Q-to-B mode transition process. Pulse fading during about 15 stellar rotations preceding the appearance of subpulse drift was observed as well. Another part of the work is devoted to a detailed study of the pulse polarization variations in the main modes. A complex behaviour of the linear polarization percentage (LPP) of the dominant first component of the average profile with B-mode age has been found: during the first 4 h, the LPP continuously increases from 5 to 40 per cent, and over the next 1.5 h gradually decreases down to 30 per cent until the subsequent onset of the Q mode. In contrast, the LPP of the second component does not change over the B-mode lifetime, remaining at the level of 22 per cent. A non-instantaneous decrease in the LPP was detected at Q-mode onset. No systematic change of the LPP of the averaged Q-mode pulses over several hours of age was found. The results are discussed within the framework of the core–cone beam model and orthogonal polarization modes.

Interactions between cathodic- and anodic-pulses during high-frequency stimulations with the monophasic-pulses alternating in polarity at axons—experiment and simulation studies

Background. Electrical neuromodulation therapies commonly utilize high-frequency stimulations (HFS) of biphasic-pulses to treat neurological disorders. The biphasic pulse consists of a leading cathodic-phase to activate neurons and a lagging anodic-phase to balance electrical charges. Because both monophasic cathodic- and anodic-pulses can depolarize neuronal membranes, splitting biphasic-pulses into alternate cathodic- and anodic-pulses could be a feasible strategy to improve stimulation efficiency. Objective. We speculated that neurons in the volume initially activated by both polarity pulses could change to be activated only by anodic-pulses during sustained HFS of alternate monophasic-pulses. To verify the hypothesis, we investigated the interactions of the monophasic pulses during HFS and revealed possible underlying mechanisms. Approach. Different types of pulse stimulations were applied at the alvear fibers (i.e. the axons of CA1 pyramidal neurons) to antidromically activate the neuronal cell bodies in the hippocampal CA1 region of anesthetized rats in-vivo. Sequences of antidromic HFS (A-HFS) were applied with alternate monophasic-pulses or biphasic-pulses. The pulse frequency in the A-HFS sequences was 50 or 100 Hz. The A-HFS duration was 120 s. The amplitude of antidromically-evoked population spike was measured to evaluate the neuronal firing induced by each pulse. A computational model of axon was used to explore the possible mechanisms of neuronal modulations. The changes of model variables during sustained A-HFS were analyzed. Main results. In rat experiments, with a same pulse intensity, the activation volume of a cathodic-pulse was greater than that of an anodic-pulse. In paired-pulse tests, a preceding cathodic-pulse was able to prevent a following anodic-pulse from activating neurons due to refractory period. This indicated that the activation volume of a cathodic-pulse covered that of an anodic-pulse. However, during sustained A-HFS of alternate monophasic-pulses, the anodic-pulses were able to prevail over the cathodic-pulses in activating neurons in the overlapped activation volume. Model simulation results show the mechanisms of the activation failures of cathodic-pulses. They include the excessive membrane depolarization caused by an accumulation of potassium ions, the obstacle of hyperpolarization in the conduction pathway and the interactions from anodic-pulses. Significance. The study firstly showed the domination of anodic-pulses over cathodic-pulses in their competitions to activate neurons during sustained HFS. The finding provides new clues for designing HFS paradigms to improve the efficiency of neuromodulation therapies.

Study of Bipolar Inductively Isolated High-Voltage Pulse Source

To meet the application requirements of pulsed power technology in wastewater treatment and other aspects (for example, a scenario that requires a high-voltage pulse source that can output a pulse with a rising edge in the nanosecond range and a device that can stabilize the pulse under high-frequency conditions), this paper designed an inductively isolated bipolar high-voltage pulse source. It consists of three parts: the primary charging power supply, the isolated driver circuit, and the pulse-forming circuit. By using inductance instead of traditional resistance isolation, using the inductance for charging and isolation, the inductance can increase the charging voltage of the capacitor, so the circuit can achieve a higher voltage output. The dual-Marx generator parallel connection design of the pulsed power supply topology, using a primary charging power supply for positive and negative polarity charging, can simultaneously output the reverse polarity and size of high-voltage pulses to achieve a bipolar output, so the load has an effect on the pulse. The equipment was selected on the basis of the design of the isolated driver circuit, primary charging power supply circuit, and pulse-forming circuit for the corresponding simulation, and in the experiment to build a principle model, the tests showed that the pulse source output pulse amplitude range was 0~10 kV (the positive pulse voltage was 5 kV, and the negative pulse voltage was −5 kV), the pulse rising edge was approximately 200 ns, the pulse width was 1 μs, and the frequency range was 0~1 kHz for the pulse source frequency. The power supply was designed to be used in applications such as wastewater treatment.

Effect of nanosecond pulsed electric fields (nsPEFs) on coronavirus survival

Previous work demonstrated inactivation of influenza virus by GHz frequency electromagnetic fields. Despite theoretical and experimental results, the underlying mechanism driving this inactivation remains unknown. One hypothesis is that the electromagnetic field is causing damage to the virion membrane (and therefore changing spike protein orientation) rendering the virus unable to attach and infect host cells. Towards examining this hypothesis, our group employed nanosecond pulsed electric fields (nsPEFs) as a surrogate to radiofrequency (RF) exposure to enable exploration of dose response thresholds of electric field-induced viral membrane damage. In summary, Bovine coronavirus (BCoV) was exposed, in suspension, to mono and bipolar 600-ns pulsed electric fields (nsPEFs) at two amplitudes (12.5 and 25 kV/cm) and pulse numbers [0 (sham), 1, 5, 10, 100, and 1000] at a 1 Hz (Hz) repetition rate. The temperature rise immediately after exposure(s) was measured using thermocouples to differentiate effects of the electric field (E-field) and heating (i.e., the thermal gradient). Inactivation of BCoV was evaluated by infecting HRT-18G host cells and assessing differences in virus infectivity days after exposure. Our results show that 600 nsPEFs, both bipolar and monopolar, can reduce the infectivity of coronaviruses at various amplitudes, pulse numbers, and pulse polarity. Interestingly, we observed that bipolar exposures appeared to be more efficient at lower exposure intensities than monopolar pulses. Future work should focus on experiments to identify the mechanism underlying nsPEF-induced viral inactivation.

Study of trigger mechanisms of a pseudospark switch configuration with a steady-state auxiliary glow discharge

This paper presents data on investigation of the pseudospark switch whose trigger unit uses a low-current auxiliary glow discharge with hollow cathode and hollow anode. The electrode system of this unit communicates with the main high-voltage gap of the switch due to the aperture in the flat part of one of the trigger electrodes. It is shown that when the electrode with the aperture plays of role of the hollow cathode of the auxiliary discharge, a low parasitic current through the aperture is provided by the ion flow. This ion current does not practically influence on the static breakdown voltage of the main gap. Applying the trigger pulse between the trigger electrodes leads to the pulsed trigger discharge with an enhanced current. At a negative polarity of the trigger pulse, the cavity with aperture turns out into the hollow anode so that the current is able to be intercepted through the aperture to the grounded cavity of the main gap. The plasma is generated in the cavity and the switch is triggered. With the proper selection of the geometry of the trigger electrodes, the delay time to trigger at a level of several tens of nanoseconds is achievable.

Deriving Metastable Pourbaix Diagrams of Stainless Steels Using Density Functional Theory Calculations

This paper presents a novel method for deriving metastable Pourbaix diagrams of Fe-Cr alloys using Density Functional Theory calculations, with the objective of providing a more comprehensive understanding of the thermodynamic equilibria within passive films, considering the metallurgical characteristics of the alloys and the electrochemical polarization conditions. By using advanced numerical tools and optimization algorithms provided by the Materials Project Database, we have developed an approach that incorporates nonequilibrium effects as a corrective factor for the chemical equilibria observed in passive films. Our findings highlight the significant role of nucleation conditions, particularly for the Cr2O3 oxide, in stabilizing the inner layers of the passive films. Lastly, the method was used to investigate the impact of pulsed polarization conditions on AISI 304 stainless steel and proposed a reaction scheme elucidating the enhanced corrosion protective properties of the passive film.

Large aperture and defect-free liquid crystal planar optics enabled by high-throughput pulsed polarization patterning.

The liquid crystal (LC) geometrical phase optics, which is realized by the high-resolution control of the optical axis orientation in transparent micrometer-thin polymer films, is emerging as a next generation of planar optics. It features pronounced optical properties and stimuli-responsive behaviors, which could introduce appealing and new possibilities for photonic purposes. The development of fabrication techniques producing elements with large aperture sizes and arbitrarily varying molecular orientation is of significance in terms of practical utility. Here, we propose the pulsed polarization patterning technique to create large-aperture and defect-free LC geometrical phase elements. We investigated the capability of the azo photo-alignment material responding to nanosecond laser pulses and the corresponding anchoring behaviors to LCs. The threshold was reduced to one fourth of that under the continuous wave recording. The patterning resolution was found to be enhanced to around 0.71 µm, due to the ultra-fast interaction nature of the photo-alignment material with the polarized light field. We proposed the flying exposure mode to deliver high frequency modulated polarized laser pulses (8 kHz), with the precision stage moving in a uniform velocity for light-field stitching and the servo auto-focusing in the sample normal, enabling the stable and reliable polarization patterning for large aperture sizes. We further report on representative fabrication of LC polarization gratings with an aperture of 4 inch and 99.2% average diffraction efficiency.

Terahertz unipolar polarimetry by second-harmonic generation in air

Femtosecond laser-based terahertz (THz) sources have gained attention for their potential in various applications. As for any electromagnetic radiation, the wave polarization is a critical parameter, which needs to be under control in a wide class of those applications. However, characterizing the polarization of broadband THz pulses remains challenging due to their limited efficient optics. THz air-biased coherent detection has emerged as a promising candidate. The technique employed is heterodyne detection, utilizing second-harmonic generation induced by THz radiation in laser-induced air plasmas. This approach provides exceptional spectral bandwidth and an unbounded power detection limit, rendering it highly suitable for ultra-broadband and high-power THz sources. It enables a very efficient polarization measurement too. However, recent findings have revealed that the laser-induced air plasma generated in this technique can exhibit birefringence, which in turn adds systematic errors to the polarization-state determination. In this Letter, we propose a simplified approach that utilizes a weak probe beam and avoids high-voltage DC bias-fields. Unlike the terahertz (THz) air-biased coherent detection scheme, our approach yields a unipolar, intensity-proportional signal for second-harmonic generation. The experimental results reported in this Letter show the absence of induced birefringence in air and, hence, demonstrate accurate measurements of the polarization state of ultra-broadband THz pulses. Therefore, our technique may provide valuable results in applications where the polarization state, and not the full electric field waveform, is required for analysis or characterization. Finally, we discuss a possible application of our method to the emergent field of THz singular optics.

A Fully Integrated 0.6 Gbps Data Communication System for Inductive-Based Digital Isolator with 0.8 ns Propagation Delay and 10−15 BER

Digital isolators are implemented to protect low-voltage electronics and ensure human safety during high-voltage surge events. In this work, we present the design of an inductive-based digital isolation system that can sustain up to 1 kVrms breakdown voltage. The proposed system is designed using the pulse polarity modulation scheme and fabricated in a 0.35 μm CMOS. Two identical dies are bounded within the IC package, with one die housing the transmitter (Tx) and the isolation transformer, while the other die contains the receiver (Rx). Two different customized designs between three metal layers are implemented to form the isolation element. The transformer’s secondary coil is constructed in metal-1, while the primary coil is formed in metal-2 and metal-3 for comparing the system functionality, isolation capability, and propagation delay. The functionality has been verified by measurements for an operating frequency of 300 MHz with a 2.6 ns propagation delay and an energy consumption of 8.15 × 103 pJ/bit at 1 Mbps. The chip was tested under extreme temperatures and achieved a maximum measured common mode transient immunity (CMTI) of 500 V/μs. Jitter has been examined to ensure fast transmission at a bit error rate (BER) of 10−15 with a total jitter (TJ) of 188.18 ps.

Electronic dispersion, correlations and stacking in the photoexcited state of 1T-TaS2

Here we perform angle and time-resolved photoelectron spectroscopy on the commensurate charge density wave (CDW) phase of 1T-TaS2. Data with different probe pulse polarization are employed to map the dispersion of electronic states below and above the chemical potential. Upon photoexcitation, the fluctuations of CDW order erase the band dispersion and squeeze the electronic states near to the chemical potential. This transient phase sets within half a period of the coherent lattice motion and is favored by strong electronic correlations. The experimental results are compared to density-functional theory calculations with a self-consistent evaluation of the Coulomb repulsion. Our simulations indicate that the screening of Coulomb repulsion depends on the stacking order of the TaS2 layers. The entanglement of such degrees of freedom suggest that both the structural order and electronic repulsion are locally modified by the photoinduced CDW fluctuations.

Control of the Electroporation Efficiency of Nanosecond Pulses by Swinging the Electric Field Vector Direction.

Reversing the pulse polarity, i.e., changing the electric field direction by 180°, inhibits electroporation and electrostimulation by nanosecond electric pulses (nsEPs). This feature, known as "bipolar cancellation," enables selective remote targeting with nsEPs and reduces the neuromuscular side effects of ablation therapies. We analyzed the biophysical mechanisms and measured how cancellation weakens and is replaced by facilitation when nsEPs are applied from different directions at angles from 0 to 180°. Monolayers of endothelial cells were electroporated by a train of five pulses (600 ns) or five paired pulses (600 + 600 ns) applied at 1 Hz or 833 kHz. Reversing the electric field in the pairs (180° direction change) caused 2-fold (1 Hz) or 20-fold (833 kHz) weaker electroporation than the train of single nsEPs. Reducing the angle between pulse directions in the pairs weakened cancellation and replaced it with facilitation at angles <160° (1 Hz) and <130° (833 kHz). Facilitation plateaued at about three-fold stronger electroporation compared to single pulses at 90-100° angle for both nsEP frequencies. The profound dependence of the efficiency on the angle enables novel protocols for highly selective focal electroporation at one electrode in a three-electrode array while avoiding effects at the other electrodes. Nanosecond-resolution imaging of cell membrane potential was used to link the selectivity to charging kinetics by co- and counter-directional nsEPs.

Large energy polarization convertible solitons in an all-PM mode-locked Yb-doped fiber laser system

In this paper, we have demonstrated an all polarization maintaining (PM) composite ultrafast Yb-doped fiber laser system mode-locked by semiconductor saturable absorber mirror (SESAM), which incorporates the amplifier with the resonant cavity together. Benefiting from this composite laser system, not only the pulse energy is significantly improved, but also polarization convertible solitons are realized. When the pump power is lower than 272 mW, the solitons with the central wavelength of 1029.78 nm and the pulse width of 16.54 ps are transmitting along the fast axis. However, the polarization direction of solitons is switched to slow axis as increasing the pump power to be larger than 272 mW. The maximum output power of the solitons can reach 154.93 mW, corresponding to the pulse energy of 9.53 nJ. To the best of our knowledge, this is the first time to realize 90° polarization conversion for ultrafast solitons in an all-PM fiber laser system. We believe the presented approach reveals a pulse scaling and polarization convertible potential of mode-locked fiber laser.

An Acoustoelectric Approach to Neuron Function

An acoustoelectric approach to neuron function is proposed that combines aspects of the widely accepted electrical-circuit-based Hodgkin–Huxley model for the generation and propagation of action potentials via electric polarization with mechanical models based on propagation via capillary waves. Explaining measured velocities of action potentials quantitatively, it also predicts the electrical tunability of highly anisotropic polarization packages that surf on the dynamic mechanical force field deforming the neuron membrane. It relies substantially on the local motion of dipoles formed by excess charges close to the inside surface of the neuron membrane, which in turn are anisotropically screened by water molecules in their hydration shell, thus modulating the strong electric field at the interface. As demonstrated on acoustic resonators of suspended nanowires fabricated out of amorphous dipolar silicon nitride, high electric fields combined with predominantly axial-strain modulation can cause transverse acoustoelectric polarization waves that propagate soliton-like with extremely low loss. In neurons, the modulation of electric polarization is confined in the nanometer-thin skin of a high electric field inside the neuron membrane and propagates phase-coherent along the axon as a lowest-order one-dimensional breathing mode, similar to transverse polarization pulses studied in nanowire resonators. Some experiments for the further manifestation of the model as well as topological protection of such breathing-mode polarization waves are discussed.

The influence of asymmetrical bipolar pulses and interphase intervals on the bipolar cancellation phenomenon in the ovarian cancer cell line

The application of negative polarity electrical pulse (↓) following positive polarity pulses (↑) may induce bipolar cancellation (BPC), a unique physiological response believed to be specific to nanosecond electroporation (nsEP). The literature lacks analysis of bipolar electroporation (BP EP) involving asymmetrical sequences composed of nanosecond and microsecond pulses. Moreover, the impact of interphase interval on BPC caused by such asymmetrical pulse needs consideration. In this study, the authors utilized the ovarian clear carcinoma cell line (OvBH-1) model to investigate the BPC with asymmetrical sequences. Cells were exposed to pulses delivered in 10-pulse bursts but as uni- or bipolar, symmetrical, or asymmetrical sequences with a duration of 600 ns or 10 µs and electric field strength equal to 7.0 or 1.8 kV/cm, respectively. It was shown that the asymmetry of pulses influences BPC. The obtained results have also been investigated in the context of calcium electrochemotherapy. The reduction of cell membrane poration, and cell survival have been observed following Ca2+ electrochemotherapy. The effects of interphase delays (1 and 10 µs) on the BPC phenomenon were reported. Our findings show that the BPC phenomenon can be controlled using pulse asymmetry or delay between the positive and negative polarity of the pulse.