Garden Plants Research Articles

Mutual effects of humic acid content and nitrogen sources for vegetative development and flowering of snapdragon (Antirrhinum majus L.)

Snapdragon (Antirrhinum majus L.), a garden plant cherished for its unique and colorful flowers, is widely used in bouquets and wreaths as a cut flower. The purpose of this study is to examine the effects of nitrogen sources (ammonium sulfate, AS; ammonium nitrate, AN; and urea) and humic acids from lignite sources (TKI-Humas and HUM-Zn) on the growth and flower production of snapdragon that are grown in pots under controlled conditions. It has been observed that plants started to flower during applications of HUM-Zn with AN and urea, whereas they remained at the vegetative stage during the application of HUM-Zn with AS. Furthermore, it has been determined that the two humic acid sources with AS usage prolonged the vegetative development and did not induce flowering of plants. Simultaneous application of humic acid and nitrogen sources has caused an increase in the leaf width, peduncle diameter, floret weight, chlorophyll content, and biomass of the snapdragon. Results show that the application of HUM-Zn with AN and urea has been effective on the plant’s vegetative organs, flowering, and dry weight. It indicated that HUM-Zn contains zinc, which is effective in flowering and biomass development. In conclusion, it was concluded that the simultaneous application of humic acid with AN or urea rapidly affected the flowering process of snapdragon.

Losing foreignness: Johann Sigismund Elsholtz on the meaning of plants in the pleasure gardens of Berlin

As a result of trade and commerce in global empires, plants from around the world could be found growing in gardens throughout Europe, including Germany. Johann Sigismund Elsholtz (1623–1688), a member of the Leopoldina scientific academy, would never personally travel to the Americas or Asia, but he had direct experience with plants from abroad under his care in the pleasure gardens of Berlin. Elsholtz composed several editions of a work that focused on the role of climate in growing a garden. For it was the climate, and not so much the soil, that posed the most significant challenge for the gardens he supervised, especially for the foreign plants. Curiously, Elsholtz's work is selective on which plants it categorizes as foreign; the category is also malleable, and proper acclimation of a plant was a first step for it no longer to be called foreign. In this paper, I explore the question of how plants lose their foreignness and take on a new, domestic meaning.

Can gardeners identify ‘future invaders’?

It is estimated that there are 30 million gardeners in Britain, who could play a crucial role in being the ‘first contact’ for reporting ornamental plants in gardens with invasive potential. Invasive species are one of the five drivers of the global nature crisis, many of which were originally introduced through ornamental horticulture. Ornamentals confined to gardens and those which have already naturalised, but are not yet shown to be invasive, represent a ‘pool’ of species with invasive potential – ‘future invaders’. An online survey asking gardeners to report ornamentals they had noticed invading or taking over their garden resulted in 251 different taxa being reported (including cultivars). The future invaders were prioritised with a simple yet structured scheme, looking at the domestic and global naturalised and invasive status of each taxon, including in the Global Register of Introduced and Invasive Species (GRIIS) and the Global Naturalized Alien Flora (GloNAF) databases. The structured scheme identified a shortlist of nine ornamentals of concern which should be prioritised for further analysis, such as a formal risk assessment. Identifying and preventing future invaders before they escape gardens is critical, to prevent future threats to nature. There is also a gap in the identification of potentially invasive ornamentals, which are not currently invasive, yet are beyond the scope of formal horizon scanning because they are naturalised. Here we explore whether surveying gardeners can be a suitable approach to prioritising future invaders while also being an opportunity to increase awareness of invasive species. This positive feedback loop between gardeners and invasion scientists could help reduce the risk of future invaders.

The Precious Potential of the Sacred Tree Chamaecyparis obtusa (Siebold & Zucc.) Endl. as a Source of Secondary Metabolites with Broad Biological Applications.

Chamaecyparis obtusa (Siebold & Zucc.) Endl., which belongs to the Cupressaceae family, occurs naturally in North America and Asia, especially in Korea, Taiwan and Japan, where it is an evergreen, coniferous, sacred, ethnic tree. It has many useful varieties that are widespread throughout the world and grown for decorative purposes. It is most commonly used as an ornamental plant in homes, gardens or parks. It is also widely used in many areas of the economy; for example, its wood is used in architecture as well as furniture production. In addition, oil extracted from Chamaecyparis obtusa is increasingly used in cosmetology for skin care. Due to its wide economic demand, mainly in Japan, it represents the largest area of plantation forest. Despite this, it is on the red list of endangered species. Its use in ethnopharmacology has led to more and more research in recent years in an attempt to elucidate the potential mechanisms of its various biological activities, such as antimicrobial, antioxidant, anticancer, antidiabetic, antiasthmatic, anti-inflammatory, antiallergic, analgesic and central nervous system effects. It has also been shown that Chamaecyparis obtusa can be used as an insect repellent and an ingredient in plant disease treatment. This thesis provides a comprehensive review of the biological studies to date, looking at different areas of the economic fields of potential use of Chamaecyparis obtusa.

LiNAC100 contributes to linalool biosynthesis by directly regulating LiLiS in Lilium 'Siberia'.

The transcription factor LiNAC100 has a novel function of regulating floral fragrance by directly regulating linalool synthase gene LiLiS. Lilium 'Siberia', an Oriental hybrid, is renowned as both a cut flower and garden plant, prized for its color and fragrance. The fragrance comprises volatile organic compounds (VOCs), primarily monoterpenes found in the plant. While the primary terpene synthases in Lilium 'Siberia' were identified, the transcriptional regulation of these terpene synthase (TPS) genes remains unclear. Thus, understanding the regulatory mechanisms of monoterpene biosynthesis is crucial for breeding flower fragrance, thereby improving ornamental and commercial values. In this study, we isolated a nuclear-localized LiNAC100 transcription factor from Lilium 'Siberia'. The virus-induced gene silencing (VIGS) of LiNAC100 was found to down-regulate the expression of linalool synthase gene (LiLiS) and significantly inhibit linalool synthesis. Conversely, transient overexpression of LiNAC100 produced opposite effects. Additionally, yeast one-hybrid and dual-luciferase assays confirmed that LiNAC100 directly activates LiLiS expression. Our findings reveal that LiNAC100 plays a key role in monoterpene biosynthesis in Lilium 'Siberia', promoting linalool synthesis through the activation of LiLiS expression. These results offer insights into the molecular mechanisms of terpene biosynthesis in Lilium 'Siberia' and open avenues for biotechnological enhancement of floral scent.

Crop–livestock-integrated farming system: a strategy to achieve synergy between agricultural production, nutritional security, and environmental sustainability

IntroductionClimate change, nutritional security, land shrinkage, and an increasing human population are the most concerning factors in agriculture, which are further complicated by deteriorating soil health. Among several ways to address these issues, the most prominent and cost-effective means is to adopt an integrated farming system (IFS). Integrating farming systems with livestock enables a way to increase economic yield per unit area per unit of time for farmers in small and marginal categories. This system effectively utilizes the waste materials by recycling them via linking appropriate components, thereby minimizing the pollution caused to the environment. Further integrating livestock components with crops and the production of eggs, meat, and milk leads to nutritional security and stable farmer's income generation. So, there is a dire need to develop an eco-friendly, ecologically safe, and economically profitable IFS model.MethodsAn experiment was conducted to develop a crop–livestock-based integrated farming system model for the benefit of irrigated upland farmers in the semi-arid tropics for increasing productivity, farm income, employment generation, and food and nutritional security through efficient utilization of resources in the farming system.Results and discussionThe IFS model has components, viz., crop (0.85 ha) + horticulture (0.10 ha) + 2 cattles along with 2 calves in dairy (50 m2) + 12 female goats and 1 male goat (50 m2) + 150 numbers of poultry birds (50 m2) + vermicompost (50 m2) + kitchen garden (0.02 ha) + boundary planting + supporting activities (0.01 ha) in a one-hectare area. The model recorded a higher total MEY (162.31 t), gross return (689,773), net return (317,765), and employment generation (475 mandays). Further negative emissions of −15,118 CO2-e (kg) greenhouse gases were recorded under this model. The study conclusively reveals that integration of crop, horticulture, dairy, goat, poultry, vermicompost production, kitchen garden, and boundary planting models increases the net returns, B:C ratio, employment generation, nutritional security, and livelihoods of small and marginal farmers.

Digital preservation of classical gardens at the San Su Shrine

Chinese classical gardens, an essential part of World Cultural Heritage, are well exemplified by Xishu Garden as a notable branch of this tradition. This research focuses on the San Su Shrine—a quintessential example of Xishu Garden—and explores the application of digital technologies in the commemorative research and quantitative preservation of these gardens. By integrating terrestrial laser scanning and terrestrial digital photogrammetry, we acquired quantitative data and constructed digital models to innovate upon the methodological approach to garden conservation. The innovation of this study is its analysis of Xishu Garden’s commemorative spaces through both temporal and spatial dimensions. 1) Temporally, this study examines the evolution and stratification of the garden's commemorative elements across various historical periods. 2) Spatially, it explores the interconnectedness of commemorative elements within the garden's buildings, rockery stones, water landscape and plant landscape. This dual-faceted approach yields novel insights and methods for assessing the universal value of the commemorative aspects of Xishu Garden. Moreover, it promotes the application of digital technology in the study and preservation of Xishu and other Chinese classical gardens.

Microbial changes and associated metabolic responses modify host plant adaptation in Stephanitis nashi.

Symbiotic microorganisms are essential for the physiological processes of herbivorous pests, including the pear lace bug Stephanitis nashi, which is known for causing extensive damage to garden plants and fruit trees due to its exceptional adaptability to diverse host plants. However, the specific functional effects of the microbiome on the adaptation of S.nashi to its host plants remains unclear. Here, we identified significant microbial changes in S.nashi on 2 different host plants, crabapple and cherry blossom, characterized by the differences in fungal diversity as well as bacterial and fungal community structures, with abundant correlations between bacteria or fungi. Consistent with the microbiome changes, S.nashi that fed on cherry blossom demonstrated decreased metabolites and downregulated key metabolic pathways, such as the arginine and mitogen-activated protein kinase signaling pathway, which were crucial for host plant adaptation. Furthermore, correlation analysis unveiled numerous correlations between differential microorganisms and differential metabolites, which were influenced by the interactions between bacteria or fungi. These differential bacteria, fungi, and associated metabolites may modify the key metabolic pathways in S.nashi, aiding its adaptation to different host plants. These results provide valuable insights into the alteration in microbiome and function of S.nashi adapted to different host plants, contributing to a better understanding of pest invasion and dispersal from a microbial perspective.

Cultural connotation and bionic design application of foliage plants in Chinese gardens

Since the dawn of time, foliage plants have been the primary plant material used in the design of Chinese landscape gardens, and their rich cultural and historical heritage can serve as inspiration for design improvement and innovation. This study categorizes the decorative traits of foliage plants into three categories, aiming to explore their cultural characteristics and design applications. Special attention is given to the folkloric, cultural, religious, and totemic meanings of foliage plants. The various forms of bionic design based on the application of foliage plants are categorized and illustrated with specific cases. This exploration includes perspectives of morphology, texture, function, and imagery, all rooted in the theory of bionic design. The beauty of natural art, the humanistic attitude, and the advancement of function, material, and technology in product design can all be reflected in foliage plants as design elements.

Effect of sludge resulting from biocoagulation-flocculation process on Abelmoschus esculentus L. growth

The current study was performed to determine the effect of sludge resulting from biocoagulation-flocculation process on okra (Abelmoschus esculentus L.) growth. The effectiveness of the resultant sludge collected after coagulation-flocculation of aquaculture effluent using biocoagulant-flocculant was compared with garden soil (nutrient rich medium) and also with sand (nutrient deficiency medium). The growth of plants in terms of physical observation and also the agronomic parameters (the number of leaves and the height of plants) was monitored for 8 weeks. At the end of the exposure period, there is no significant difference in terms of the number of leaves and also the height of plants between plants in garden soil and plants in pots with the resultant sludge. In contrast, the agronomic parameters for plants in pot with sludge were significantly higher than plants in pot with sand only. As for the relative growth rate (RGR) of plants, plants in pots with sludge were the highest (3.84 g/week) compared to garden soil (3.52 g/week) and sand only (0.25 g/week). It can be concluded that sludge generated from the coagulation-flocculation process of aquaculture effluent using natural resources as coagulant-flocculant agent has given positive impact on the growth of okra plants and is potentially utilized as biofertilizer for plants.

Chisocheton pentandrus (Blanco) Merr. leaf as a potential antioxidant and α-glucosidase inhibitory agent

Indonesia is one of the countries with the highest diversity of plants worldwide, including the Chisocheton genus. The Chisocheton genus has been widely used in traditional medicine due to a variety of compounds with different activities, such as antiplasmodial, antiviral, and anti-inflammatory. The focus of this research is to determine the antioxidant, anti-diabetic, and anti-bacterial activity of Chisocheton pentandrus leaf extract from the Bali Botanic Garden plant collection, as well as the chemical compounds contained in the extract. The antioxidant activity test was carried out by capturing DPPH-free radicals, the antidiabetic test was carried out by assaying its inhibitory activity toward α-glucosidase enzyme, the antibacterial test was carried out using the agar diffusion method, while LC-MS/MS was used to determine the compounds contained in the extract. The results showed that the C. pentandrus leaf methanol extract showed the highest inhibitory activity as an antioxidant and inhibitor of the α-glucosidase enzyme in the very strong category; however, the extract is not effective in inhibiting bacterial growth.

ACALYPHA RHOMBOIDEA IN THE VASCULAR FLORA OF ROMANIA

In this paper, Acalypha rhomboidea (Euphorbiaceae) is reported for the first time, as a neophyte, in the vascular flora of Romania. We have identified small populations of this species native to North America, in the central area of the Iași city (north-eastern Romania), as a weed in a garden of ornamental plants, near the Palace of Culture. Most likely it has been introduced here accidentally, by importing contaminated growing medium for gardens. It produces viable seeds and seems to survive here for more than 10 years despite regular weed control. The morphology of this species is described based on field and herbarium specimens, and checked on literature data. The paper also includes some data on general distribution and favourite habitats of this species, as well as two identification keys.

First documentation of diversity of the Heteroptera of Cotton University Campus, Kamrup (Metropolitan), Assam, India

Cotton University, a developing university in Guwahati city of Kamrup district from Assam is undergoing a lot of developmental changes leading to a change in land use pattern which will have an impact on insect distribution. Heteroptera studies being severely lacking from this region needs attention for their documentation. A survey conducted on the campus from July 2018 to July 2019 every week between 0700–0900 h on all types of vegetation documented a total of 163 bugs of 20 different species under 10 families of Heteroptera. Pentatomidae was found to be the most dominant family with eight recorded species. Wild vegetation supported more diverse bug forms than the other garden plants. This survey is the first record of these insects from the Cotton University campus and such data forms an important database of available bugs from this region in the wake of the developmental changes the university is going through. Any change in their distribution, if found in the future, will reflect the impact of anthropogenic activities on their existence and distribution. Studies of this kind may be minuscule in level, but it forms an important record of their presence before we lose them to time.

Medicinal intercultural plant garden: homeopathy and phyto-nutritional care

The present article describes historic evolution conception process of an intercultural medicinal plant garden as a tool to construct social ecoperception about environmental health and cultural diversity, to improve environmental ethics. Through case study methodology it reports the process along five years. Hybrid Learning of Homeopathy, Phytotherapy, Bioethic were successful to scientific basic research initiation and novel biological research will be done using pathogenetic chemical markers to discuss biologic results. Results also show multi, inter and transdisciplinary effective activities and communications about primary care attention. Empirically, it has been developped a positive perception among patients, students and professionals about Homeopathy, as a qualitative humanized approach. Phytotherapy was mainly preconized by its nutraceutical, functional and adaptogenic effects. Indeed, intercultural medicinal plant garden construction process provided integrality of applied intercultural, interethnical and indigenous knowledge expressed from their affective memories and registered like an “open-air” intercultural plant collection (“plant library”), improving humanities as respect, ethic, kindness and integrated citizenship, besides the learning to coexist with complexity, singularity and self-direction without lost of professional ethic. So, as qualitative evaluation, it was considered that a mandate of equity, social justice and welfare to mental health promotion to this urban ethnic members and indigenous, as recommended in health promotion by the Ottawa Charter, has been fulfilled in this period (2018 – 2023) at this intersectoral ambulatory (which necessary includes medical anthropology and clinical bioethics studies) at Homeopathy Service, 7a Yard of General Hospital Santa Casa da Misericordia - Rio de Janeiro/Brazil.

Dust Retention Effect of Greenery in Typical Urban Traffic Landscapes of Nanjing—In the Case of Xuanwu Avenue in Nanjing City

With the accelerated process of urbanization, air pollution has become increasingly severe. Garden plants can trap atmospheric particulate matter, which is of great significance for improving the urban ecological environment and promoting sustainable development. To investigate the dust retention effect of typical transportation green spaces in Nanjing, this study focuses on thirteen garden plants on Xuanwu Avenue in Nanjing. The dust retention capacity of these plants was determined using the wash-off method, while the microstructure of their leaf surfaces was observed using scanning electron microscopy. The results are as follows: Firstly, per unit leaf area, Liriope spicata, Ophiopogon japonicus, and Viburnum odoratissimum demonstrate solid dust retention abilities. Additionally, Viburnum odoratissimum, Prunus serrulata var. Lannesiana, and Liriope spicata show strong dust retention abilities per single leaf. Moreover, Platanus acerifolia, Viburnum odoratissimum, and Cinnamomum camphora have strong dust retention abilities per plant. Viburnum odoratissimum, Platanus acerifolia, and Prunus serrulata var. Lannesiana exhibit the most substantial dust retention capacities. Secondly, there is a significant negative correlation between dust retention per plant and the potassium content, while a significant positive correlation is observed with plant height, canopy height, and leaf width. Furthermore, there is a highly significant positive correlation between dust retention per unit leaf area and stomatal length and a highly significant negative correlation with leaf length. The surface microstructure of the blade mainly increases the dust retention capacity of the blade by increasing the friction of the leaf surface. Lastly, specific leaf surface microstructures, such as grooved epidermis and trichomes, enhance plants’ dust retention capacity. Consequently, for the future configuration of road green spaces in Nanjing, a mixed planting mode of trees, shrubs, and grass is recommended. Priority should be given to selecting plants with strong overall dust retention capabilities, such as Platanus acerifolia, Viburnum odoratissimum, and Prunus serrulata var. Lannesiana, to alleviate air pollution, improve the urban ecological environment, and achieve sustainable development.

Spatiotemporal Distribution Analysis of Spatial Vitality of Specialized Garden Plant Landscapes during Spring: A Case Study of Hangzhou Botanical Garden in China

Specialized gardens, as integral components of botanical gardens, bear multiple functions, encompassing plant collection and conservation, scientific research, and public education, as well as serving aesthetic and recreational purposes. Their quality profoundly reflects the landscape artistry of botanical gardens, directly influencing the quality of visitors’ enjoyment and the overall experience within the botanical garden. This study aims to investigate the spatial vitality of specialized garden plant landscapes, effectively assessing the usage patterns of plant landscape spaces and promoting the optimal utilization of underutilized spaces. Taking Hangzhou Botanical Garden as a case study, considering the warming climate and suitable temperatures in spring, when most plants enter the flowering period and outdoor visitor frequency increases, the primary observational period focuses on spring to measure the spatial vitality of specialized garden plant landscapes. We obtained data through field measurements and on-site observations. Specifically, We measured and recorded information on plant species, quantity, height, crown width, and growth conditions within the plots. Additionally, we employed ground observations and fixed-point photography to document visitor numbers and activity types. We quantified spatial vitality through four indicators: visitor density, space usage intensity, diversity of age group, and richness of activity type. We explored the spatiotemporal distribution patterns of spatial vitality and investigated the relationship between plant landscape characteristics and spatial vitality using variance analysis and correlation analysis. The results indicate that, in spring, the average spatial vitality index of specialized gardens ranks from highest to lowest as follows: Lingfeng Tanmei (1.403), Rosaceae Garden (1.245), Acer and Rhododendron Garden (0.449), and Osmanthus and Crape Myrtle Garden (0.437). Additionally, the spatial vitality of specialized garden plant landscapes in spring is significantly positively correlated with the ornamental period of specialized plants, characteristics of plant viewing, accessible lawn area, spatial accessibility, and spatial enclosure. Therefore, to create vibrant specialized plant landscapes, managers and planners, when engaging in the planning and design of specialized garden plant landscapes, need to fully consider and respect the visual aesthetics and functional needs of visitors. This study will serve as a theoretical reference for subsequent research on the vitality of plant landscape spaces and other small-scale spaces. It will also provide practical guidance for the construction of plant landscapes in specialized gardens within botanical gardens and other urban green spaces.

Investigation on the Application of Spring Plants and Landscape Evaluation in Changsha Area

With the slogan of "Beautiful China" put forward by the 18th National Congress of the Communist Party of China, more and more people now choose to take wedding photos outdoors, hold weddings outdoors, and celebrate wedding anniversaries outdoors. In addition to having good quality photos, taking wedding photos outdoors requires In addition to the framing building, the plant landscape created by plants that matches the atmosphere of the wedding garden is also indispensable. However, most wedding gardens in China almost rely on architecture to create a theme atmosphere, while ignoring the role of the landscape created by plants in the theme of the entire wedding garden. This article takes Liuyang River Wedding Cultural Park in Changsha City as an example, analyzes the types and ecological characteristics of garden plants in Liuyang River Wedding Garden, and summarizes the experience in the growth, management and garden application of various garden plants. Then, on this basis, we analyze and summarize the existing problems in the application of garden plants in Liuyang River Wedding Cultural Garden, and point out corresponding measures and approaches to provide guidance for similar types of landscaping.

Interspecies Kinship of Liuran: Rethinking Marshall Sahlins’s Mutuality of Being

<p>The anthropological discussions regarding the concept of mutuality of being by Marshal Sahlins have focused on the idea that kinship only occurs between humans through certain rituals or traditions. This idea is limited in considering non-human species that also potentially form interspecies kinship. Through a multispecies ethnographic approach, this article discusses interspecies kinship in the context of joint agricultural care without exchanging exact amount of payment (liuran) by female farmers in Kampung Laut District, Cilacap Regency, Indonesia. In this study, the data were collected through participant observation techniques and in-depth interviews with four female farmers as key informants who have been involved in liuran for years. The findings showed that the interspecies kinship between female farmers and rice and garden crops is like mother and child because there has been an emotional-ecological bond in the process of caring for sedimentation farming over decades, primarily through taboo practices. The process of caring for rice and garden plants together also forms a socio-economic support system among female farmers, which helps strengthen their interspecies kinship. Considering non-human species as part of the family members places them in an equal relationship with humans in contributing to the sustainability of living ecosystems.</p>

Polyploid Induction and Identification of Begonia × benariensis

Begonia × benariensis series varieties have high ornamental and economic value and are excellent varieties for large-area group planting in gardens and green areas. At present, the B. × benariensis series varieties grown in China rely on foreign imports, and the price of imported germplasm resources is expensive, which greatly increases the cost of flower enterprises. In view of this, B. × benariensis (2n = 2x = 34) was used as the material, the callus was treated with colchicine to induce polyploid plants, and the polyploid plants were subjected to morphology, DNA content identification, and stomatal identification, as well as comparisons of the morphophysiological indexes of the plants. The results showed that the polyploid was better induced with the treatment of 0.05% colchicine for 4 h, and the induction rate was 46.67%. Among the mutagenized plants, there were 42 triploids (2n = 3x = 51) and 98 tetraploids (2n = 4x = 68). The stomatal density of diploid leaves was about 1.09 times that of the triploid and 1.67 times that of the tetraploid, the defense cells of the leaves increased gradually, and the stomatal density decreased; with the increase in ploidy, the height of the plants increased, while the crown width decreased; the maximum length and width of the leaves decreased, the color of the leaves became darker, and the chlorophyll content increased. This study provides abundant variation materials and technical support for the selection and breeding of new varieties of B. × benariensis in the future.

Berkeleyomyces rouxiae Is Responsible for Black Root Rot on Multiple Milkweed Species in South Carolina

Several outbreaks of black root rot on four milkweed species occurred at a South Carolina plant nursery specialized in growing host plants of butterflies. The diseased plants included Asclepias asperula and A. incarnata in 2022 and A. speciosa and A. verticillata in 2023. Symptoms included wilting and stunting of foliage and distinct black lesions and decay on roots. Berkeleyomyces rouxiae was isolated from the diseased roots and identified based on morphological features, DNA sequences, and phylogenetic characteristics. Koch's postulates were completed to confirm its pathogenicity on A. incarnata and A. verticillata. Milkweed plants are an important food source for the monarch butterfly, an iconic North American treasure, as well as a nectar source for other pollinators. Outbreaks have caused significant plant losses at the plant nursery; however, it is unclear whether this disease could become an issue for milkweed plants in gardens and landscapes. Effective management of Berkeleyomyces species can be a challenge.