In order to develop a feasible method for measuring FIV of both spacer grid and rod, two experiments are conducted in this study. First, the simultaneous verification tests between the LDV and piezoelectric accelerometer have been performed. For the case of vibration occurring in a channel with water, the measured signal by the LDV need to be modified while not affecting the measured vibration value. The results show that the LDV is a good candidate for measuring vibration under flow conditions. Then, the LDV is employed to measure the FIV of a specified 4 × 4 rod bundle with spacer grids. Both turbulence and vortex excite the rod bundle, and a strong vortex induced resonance is found when the flow velocity is 7.35 m/s. The vibration intensity is amplifying with increasing the flow velocity. Moreover, ‘lock-in’ phenomenon and two types of vortex shedding with St numbers of 0.15 and 0.26 are found.