Phyto Constituents Research Articles

A Critical Analysis of Medicinal plants for Anti-Depressant action with their Phyto-Constituent and Mechanism of Action

Depression or stress refers to a state of sad feelings and loss of interest in pleasurable activities characterized by retardation of thoughts and actions, appetite and weight changes, restlessness as well as in sleep disturbance. All the current evidences implicate alteration in the firing pattern of a subset of biogenic amines in the central nervous system. There are adequate number of synthetic drugs used to treat depression as standard treatment for clinically depressed patient, however only 30% of patients respond satisfactorily to the existing medicines and the remaining do not attain full recovery. Many scientists are investigating on herbal drugs for mitigating this disorder that shown antidepressant properties by virtue of synergistic effect of their phyto constituents. In this review article we emphasize to give an outline of certain medicinal plants with their constituents and mechanism of action which have been explored for their antidepressant action.

Exploring Potentilla nepalensis Phytoconstituents: Integrated Strategies of Network Pharmacology, Molecular Docking, Dynamic Simulations, and MMGBSA Analysis for Cancer Therapeutic Targets Discovery.

Potentilla nepalensis belongs to the Rosaceae family and has numerous therapeutic applications as potent plant-based medicine. Forty phytoconstituents (PCs) from the root and stem through n-hexane (NR and NS) and methanolic (MR and MS) extracts were identified in earlier studies. However, the PCs affecting human genes and their roles in the body have not previously been disclosed. In this study, we employed network pharmacology, molecular docking, molecular dynamics simulations (MDSs), and MMGBSA methodologies. The SMILES format of PCs from the PubChem was used as input to DIGEP-Pred, with 764 identified as the inducing genes. Their enrichment studies have shown inducing genes' gene ontology descriptions, involved pathways, associated diseases, and drugs. PPI networks constructed in String DB and network topological analyzing parameters performed in Cytoscape v3.10 revealed three therapeutic targets: TP53 from MS-, NR-, and NS-induced genes; HSPCB and Nf-kB1 from MR-induced genes. From 40 PCs, two PCs, 1b (MR) and 2a (MS), showed better binding scores (kcal/mol) with p53 protein of -8.6 and -8.0, and three PCs, 3a, (NR) 4a, and 4c (NS), with HSP protein of -9.6, -8.7, and -8.2. MDS and MMGBSA revealed these complexes are stable without higher deviations with better free energy values. Therapeutic targets identified in this study have a prominent role in numerous cancers. Thus, further investigations such as in vivo and in vitro studies should be carried out to find the molecular functions and interlaying mechanism of the identified therapeutic targets on numerous cancer cell lines in considering the PCs of P. nepalensis.

Qualitative and quantitative analysis of siddha herbal Formulation Kabasura kudineer in various concentrations

Introduction: Siddha system is one of the ancient systems coming practised in southern part of India, North and Eastern parts of Sri Lanka. The Siddha medicines are categorised as Aga marundhugal (Internal Medicine) and Pura marundhugal (External Medicine).One among them is kudineer (Decoction). Kabasura kudineer chooranam (KSK) is a polyherbal formulation and it contains 15 ingredients. It is indicated for Iyyasuram and it used for fever with Respiratory disease. Objective and Methods: To analyse the presents of different phyto constituents, develop HPTLC fingerfrint and quantify the amount of phenols and alkaloids in Kabasura Kudineer prepared at 1/4 (sample A), 1/8 (Sample B) and 1/16 (Sample C) reduction concentrations. Result: Phytochemical analysis of Kabasura Kudineer revealed the presence of alkaloid, phenol, flavonoid and tannin in Sample A, B and C. The Rf value obtained by TLC and HPTLC fingerprint for Sample A are 0.02, 0.04, 0.79, Sample B is 0.04, 0.80 and Sample C is 0.04, 0.43, 0.051 and 0.71. The amount of Alkaloid present in Sample A, B, C 1.61 ,1.03,1.44 and phenol present in Sample A, B, C 17.62 ,19.71,18.60 Conclusion: The Analytical parameters along with TLC photo documentation, HPTLC fingerprinting profile and UV Spectrophometer in various concentration of Kabasura Kudineeer are different in different reduction concentration ratio. Further specific chemical markers can be quantified using HPTLC and the therapeutic efficacy can be explored in further studies.

Analytical Study of Shilajatu

Background Rasashastra is a branch in Ayurveda Pharmaceutics that deals with utilization of varied origin of drugs like metals minerals etc. in articulating different formulations. Among the mineral group of drugs a mineral very commonly used in designing formulations is lsquoShilajatursquo. It is a pale brown to black coloured exudate available in mountainous regions. Based on the region and mountainous area it is collected from its appearance and the composition vary. Research studies revealed it to be a combination of organic elements and inorganic substances. Vast content relating to Shilajatu is available in the treatises of Rasashastra and much research was carried out on it. However it still remains a drug of ambiguity. Therefore this analytical study was conducted to evaluate the varied composition of Shilajatu. Methods Two samples of Shilajatu were collected from two different sources one from Nepal and another from local market in India and the samples were analyzed to determine the organic constituents and also the elemental constituents. Physico chemical analysis Phyto chemical test and Energy Dispersive X-ray Spectroscopy EDAX were carried out. Results Phytochemical evaluation of aqueous extracts of Shilajatu samples demonstrated presence of carbohydrates protein tannin steroid saponin and mucilage. EDAX revealed the presence of different elements and the sample from Nepal showed the presence of more elements. Conclusion Shilajatu is a combination of organic and inorganic elements. Various phyto constituents are present and the elemental analysis showed presence of C O Na Mg S Cl and K in Indian sample and presence of C N O Na Mg Al Si Cl K and Ca in Nepal sample.

Nickel oxide nanoparticles from Sargassumwightii: Synthesis,Characterization, and biomedical applications

Antimicrobial resistance and cancer claim millions of lives each year, making it important to find effective medications. The current study focuses on the rapid and sustainable manufacture of nickel oxide nanoparticles using Sargassum wightii aqueous extract (SW-NiO NPs) as a novel antibacterial and anticancer agent. The presence of phytochemicals in the Sargassumwightii in all extract was qualitatively screened and water extract, which is found to contain abundant phytochemical was used for the biosynthesis. Thus synthsized SW-NiO nanoparticles was characterised by UV–Visible, FT-IR, XRD, SEM, and TEM spectral studies, and then screend for its invitro anti microbial activity against bacterial strains (E. coli, K. pneumoniae, S. aureus, and B. subtilis) and fungal activity with the fungus strains (C. albicans, C. tropicalis),invitro anticancer and larvicidal activity. The SW-NiO NPs possessed cubic and hexagonal structures with an average crytallite size of 15–20 nm, according to the results of the analytical characterization. SEM and TEM analysis confirms the spherical like morphology and their respective elemental composition were identified by EDAX studies. The antimicrobial activity was carried by well diffusion method.Compared to gram-negative organisms, gram-positive species were more vulnerable to the SW-NiO NPs with the zone of inhibition 17 mm. The fungal activity results showed the zone of inhibition 16 and 14 mm. It was also found that SW-NiO NPs inhibit the growth of tested fungus at higher doses. The cytotoxicity studies with liver cancer cell line through MTT assay revealed the inhibition value at 52.25 ± 0.05 µM, indicating their sensitivity to the HepG2 cell line. SW-NiO NPs made with water extract showed the highest larvicidal activity and 88.33 % death of Aedes agypti(AE). The LC50 and LC90 values for SW-NiO NPs against Aedes agypti(AE) larvae are found to be 31.420 and 134.261 ppm, respectively. The findings of the antimicrobial, larvicidal and invitro anticancer screening of this work provide compelling evidence that SW-NiO NPs made from an aqueous extract of Sargassum wightii have considerable biological applications which can be explored in detail on identifying the potential phyto constituents which are responsible for the activity.

Investigation of Cream and Ointment on Antimicrobial Activity of Mangifera Indica Extract

Abstract: Medicinal plants have curative properties due to the presence of various complex chemicals substances of different composition which are found as secondary plants metabolites in one or more parts of these plants mangifera indica linn ( MIL) is a species of mango in the an acardiaceae family. Phyto constituents in the seed extract may be responsible for the antimicrobial activity of the plants. The purpose of the study was to formulate and evaluate the antimicrobial herbal ointments and creams form extract of the antimicrobial herbal ointments and cream bases in different concentration 1%,5%,and 10% the formulation ointments and cream of MIL .we're subjected to evaluation of uniformity of weight measured of ph viscosity, spreadability acute skin irritation study stability study and antimicrobial activity. Our study shows that M.I has high potential as an ointments and creams for topical use .thus the present study concluded that formulation of the M.I are safe and efficient carrier with potent antimicrobial activity.

Effect of supplementation of Purslane-Portulaca oleracea in hypercholesterolemic subjects

Background: Portulaca oleracea (Purslane) is a medicinal plant containing diverse phyto constituents grown all over the world. Several studies have reported its pharmacological effects like, antihypertensive, anti-inflammatory antioxidant, hypoglycemic, hypocholesterolemic, hypotriglyceridemia neuroprotective effects. Methods: Dried Portulaca oleraca leaves were incorporated into spice powder/ karampodi a south India food adjunct which was used for supplementation studies for 90 days (3months) among hyper glycemic subjects after obtaining a written informed consent. Biochemical parameters like lipid profile, i.e., total cholesterol, low density lipoprotein cholesterol, triglycerides, high density lipoprotein cholesterol, very low-density lipoprotein cholesterol, kidney function test, Liver function test were assessed. Baseline information, their medical history and 24hr dietary recall was elicited from the subjects through a pretested schedule. Results: The results have shown that the total cholesterol levels and its fractions along with triglycerides were significantly decreased, HDL-C increased significantly in the test groups from pre- supplementation to during-supplementation and were stable at post-supplementation period. Conclusions: Our results indicated that the spice powder was rich in polyunsaturated fatty acid had a strong hyper cholesterolemic and hypotriglyceridemic effect.

Phytochemical Investigation and in Vitro Antioxidant Activity of Citrus Sinensis Peel Extract

The present study was undertaken to analyse the photochemical constituents both qualitatively and quantitatively in the peel extract of Citrus sinensis. The studies showed that the major phyto constituents present in the methanolic extract was hesperidin and nobiletin. The DPPH and hydroxyl radical scavenging activity of the methanolic extract was found to be better than the other extracts used in the study. Finger printing assay of the extract was carried out using HPTLC and we report the presence of two flavonoids - nobelitin and hesperidin. Keywords: C.sinensis peel, HPTLC, antioxidant, phenolic compound, phyto constituents.

Plant-Based Bioactive Phthalates Derived from Hibiscus rosa-sinensis: As In Vitro and In Silico Enzyme Inhibition.

Hibiscus rosa-sinensis is an attractive, ever-blossoming, and effortlessly available plant around the globe. The fabulous flowers of H. rosa-sinensis enjoy a significant status in folk medicine throughout the world and comprise a range of phyto constituents due to which this splendid flower owns numerous biological and pharmaceutical activities like antioxidant, antifungal, antimicrobial, anti-inflammatory, antipyretic, antidiabetic, and antifertility activity. Considering this, column chromatographic isolation of the phytoconstituents of ethyl acetate fraction of the flowers of H. rosa-sinensis was performed. A series of five phthalates including Di-n-octyl phthalate (HR1), ditridecyl phthalate (HR2), 1-allyl 2-ethyl phthalate (HR3), diethyl phthalate (HR4), and bis (6-methylheptyl) phthalate (HR5) were isolated. The structures of the isolated phthalates were elucidated by gas chromatography-mass spectrometry, 1H NMR, and 13C NMR. In silico and in vitro antidiabetic and antioxidant potential and DFT studies of isolated phthalates were carried out. In our study, isolated ligands were explored as potent antidiabetic as well as antioxidant agents as they exhibited good binding affinity (in in vitro and in silico experiments) against all selected protein targets. Compounds HR1-HR5 showed that the binding affinity value ranged from -5.9 to -5.2 kcal/mol, -5.5 to -4.3 kcal/mol, and -5.0 to -4.1 kcal/mol for target proteins 1HNY, 2I3Y, and 5O40, respectively. Among all isolated phthalates, HR5 can be a lead compound as it showed the best binding affinity with human pancreatic α-amylase (ΔG = -5.9 kcal/mol) and displayed a minimum inhibition concentration (IC50) of 11.69 μM among all phthalates. Compound HR1 was the best docked and scored compound for inhibiting glutathione peroxidase; however, HR2 possessed the lowest binding score of -5.0 kcal/mol, thus indicating the highest potential among isolated phthalates for inhibiting the superoxide dismutase. Furthermore, the top-ranked docked ligand-protein complex for each protein was assessed for stability of protein and complex mobility by molecular dynamics simulation using the IMOD server.

Therapeutic and cultural evaluation of Brahma Kamal (Saussurea obvallata (Dc.) Edgew: An endangered potential herb

Background: The essential components of the traditional medical system are plants. A medicinal plant from Uttarakhand known as Saussurea obvallata (DC.) Edgew, is considered to be endangered due to its rich cultural and medical significance. It is found at high altitudes of 3000-4800 meter in the Himalayan region. Many studies have been published on phyto constituents and its traditional uses that shown its therapeutic values. In this article all the published research work and available data on geographical and traditional uses of Brahma Kamal are compiled here. Aim: To increase understanding of S. obvallata's cultural significance, medical uses, and protection against health risks. Methods: Review a list of published original articles and review secondary data from a variety of databases, including PubMed, PubMed Central, Shodhganga, DHARA, Ayush Research Portal, Google Scholar, Medline, Embase, etc., as well as a list of published original papers. Result: Various plant parts of S. obvallata have been used for a variety of ailments, including dysentery, rheumatism, leprosy, bone fractures, nervine debilities, cerebral palsy, paralysis, sexual disorders, lung infections, urinary tract infections, leucoderma, rhinitis, and hyperthermia. Conclusion: The information in review confirms the traditional claims and contributes in providing promising baseline information for the pharmacological use of S. obvallata. The information presented here maybe beneficial for researchers, healthcare professional and pharmaceutical companies to design and develop effective medicines against microorganisms.

Characterization of Mistletoe Leaves Growing on Fig Plant Found in NISLT, Samonda, Ibadan Premises

This study investigates the chemical composition of mistletoe leaves growing on fig tree. The mistletoe leaves and the fig leaves used for this research were harvested from Fig plant. Proximate analyses, phytochemical screening, Elemental and antioxidant analysis were done on both leaves. This study has provided a scientific justification that both the Fig leaves and Mistletoe leaves contained relevant phyto constituents such as alkaloids, saponins, flavonoid, and steroids; whereas antraquinones, tannins, phenols, resins and terpenoids were completely absent. Nutritionally relevant inorganic minerals like, Calcium, magnesium and potassium were the most abundant element in both plants. Proximate constituents like carbohydrates, lipids and proteins were present in varied amounts in both leaves. Result of inhibition of free radicals in fig leave extract increases with increase in concentration of the extract. The inhibition is closely comparable with that of the standard (Vitamin C) at the same concentration. The inhibition by the extracts of mistletoe grown on fig tree is low when compared with that of Fig and that of standard. This suggests that some of the chemical characteristics of mistletoe are conferred to them by their host trees.

Evaluation of Vernonia amygdalina del. containing phyto constituents a medicinal plant compound as new potential inhibitors of Monkey pox virus using molecular docking analysis

Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms similar to those seen in the past in smallpox patients, although it is clinically less severe. With the eradication of smallpox in 1980 and subsequent cessation of smallpox vaccination, Monkeypox has emerged as the most important orthopoxviral for public health in recent years, great progress has been made in developing new antiviral drugs, and natural products, are important sources of potential and new antiviral drugs. The present study aimed to assess some biologically active compounds present in medicinal plants as potential Monkeypox inhibitors, using molecular docking methods. The Docking study was performed by Maestro 12.8. Comparing antiviral drug Tecovirimat (TPOXX) with Luteolin, Luteolin-7-o- β-glucoside, Vernodalol, Vernolepin, Vernodalin phytoconstituents present in Vernonia amygdalina del. The results demonstrate the effectiveness of this screening strategy, which can lead to rapid drug discovery in response to new infectious diseases. The Docking results with PDB Id (6LUT) receptor showed that many phytoconstituents screening compounds isolated from medicinal plant such as; Luteolin (− 3.244), Luteolin-7-o-β-glucoside (− 2.357), Vernodalol (− 2.089), Vernolepin (− 1.757), Vernodalin (-1.534) when compared with antiviral drug Tecovirimat whose docking score (− 0.162) these compounds results might be used to inhibit Monkeypox infection an important source for novel antiviral drugs targeting Monkeypox virus.

Phytochemical investigation and pharmacological evaluation of Anti-inflammatory activity of Vitex negundo linn bark extracts

Asthma is a disease characterized by acute exacerbation of coughing dysponea, wheezing and chest tightness. Patients usually have reduced forced expiration volume in one sec as well as reduced airflow The current study aimed to evaluate the phytochemical investigation and pharmacological evaluation of anti-inflammatory activity of Vitex negundo linn bark extracts. The results when compared with 0 Hr reading of same group at specific dose levels tested (200 mg/kg, 400mg/kg and 600 mg/kg), it was observed that methanolic extract of Vitex negundo linn bark started showing the protection after 2 hrs at 200 mg/kg and 600mg/kg whereas 400 mg/kg showed at the end of 1 hr itself and continued till the end of 5 hrs and quite comparable with that of Ibuprofen. These results are encouraging enough to pursue bioactivity-guided fractionation of this extract and structure elucidation of the active phyto constituents.

Phytochemical analysis and anticancer activity of Majidea zanguebarica Kirk Ex Oliv

Phytochemicals are secondary metabolites that are naturally produced by plants. The main aim of the study is to investigate the presence of phyto constituents in the flowers of Majidea zanguebarica through GC-MS analysis. Phytochemical screening was carried out using methanol, benzene, petroleum ether and chloroform extracts which revealed the presence of alkaloids, flavonoids, steroids, tannins, saponins, fatty acids, carbohydrates and cardio glycosides. GC-MS analysis of the methanolic flower extract evidenced that the presence of 28 compounds in the flowers of M. zanguebarica. The highest amount of carbohydrate and amino acid content were present in the methanolic flower extract 0.175 mg/g and 0.269 mg/g respectively. In vitro antioxidant activity such as DPPH radical scavenging activity, hydroxyl radical scavenging activity, total antioxidant activity, reducing power of the methanolic extract of flowers were analyzed. Radical scavenging activity was found to be higher in the methanolic extract of flowers. In MTT assay, IC50 value for methanolic flower extract found to be 25 µg/ml concentration. In DNA fragmentation study, the maximum DNA fragments was observed in the high concentration of methanolic flower extract. It was concluded that the methanolic flower extract has the tendency to activate the cell death process in the treated cells. This investigation might be further evaluated for its anticancer potential in a significant level.

Study on phytochemical, antioxidant and analgesic activity of ethanolic extract of Curcuma Amada Roxb Rhizome

Introduction: Curcuma amada Roxb. usually referred as ambahaldi is an exceptionally aromatic rhizomatic herb which contains number of phyto constituents responsible for several pharmacological activities. Present study is an attempt to explore antioxidant and analgesic activity of ethanolic extract of C. amada. Objectives: The goal of this work was to assess the antioxidant and analgesic activity of ethanolic extract of C. amada. Methods: For in-vitro antioxidant study, the DPPH (2, 2-Diphenyl-1-picrylhydrazyl) free radical scavenging test and nitric oxide scavenging assay was used to assess the antioxidant activity while the in-vivo analgesic efficacy was evaluated by using tail flick method and formalin induced pain in experimental animals. Different doses of 100mg/kg, 200 mg/kg, and 400 mg/kg was used to evaluate analgesic activity of ethanolic extract of C. amada. Results: Ethanolic extract of C. amada exhibited significant antioxidant in DPPH and nitric oxide scavenging assay. 100 mg/kg, 200 mg/kg, and 400 mg/kg of ethanolic extract of C. amada produced significant analgesic activity in tail flick and formalin induced pain model in experimental animals. Conclusion: Ethanolic extract of C. amada exhibited significant antioxidant as well as in-vivo analgesic activity in experimental animals.

Anticancer Activity of Phyto Ligands from Carica papaya Leaves by Suppression of PI3CKA and BCL2 Proteins- An insilico Approach

The plant derived compounds possess several medicinal property including anticancer activities. In the present investigation molecular docking analysis was performed to identify a suitable antagonistic ligand from the phyto ligands of Carica pappya leaves which can inhibit the tumor progressive proteins PIK3CA, BCL 2. The molecular Docking analysis was performed using Autodock 4.2. The protein PIK3CA, BCL 2 structures were retrieved from PDB, and by GC-MS analysis the phyto molecules were identified. The ligand chemical structures were drawn using Chem sketch. The enzyme and ligand interaction were obtained as docking score using the Arguslabs server. Based on the docking score the best ligand was selected from the phyto constituents of Carica papaya ethanolic leaf extract and their inhibitory potential was analyzed in terms of their interactions with the amino acid residues present in the active site which were visualized and further confirmed by PYMOL. The standard drug Doxorubicin was also subjected to docking for comparison in the present study. Based on the docking score the phytochemicals namely Hexadecanoic acid, ethyl ester, Coumarine 3-(2,4-dinitrophenol), Androst-4-en-3-one,17-methoxy, 3-methoxime serves as the best antagonistic ligand in terms of their interaction with amino acids as well as inhibition of the particular tumour progressive proteins.

Concept of Unani Jali (detergents/cleansers) drugs and its scientific validation: scope for new opportunities in dermatological pharmacotherapeutics.

The use of detergent-action drugs in traditional Unani therapeutic intervention has been a long-standing Unani medicinal practice. The key aim of the article is to provide thorough information on the novel, unexplored idea of Unani Jali (detergent/cleansers) drugs for the treatment of skin ailments, as well as to identify medicinal plants that have detergent action and correlate these findings with scientific studies that may support evidence for the drug's detergent effect. The ethnobotanical classical literature of Unani medicine was investigated in order to have a comprehensive insight of Unani detergents/cleansers. Scientific studies were carried from databases including PubMed, Scopus, Science Direct, and google Scholar, among others. More than fifty exclusive plant, mineral, and animal-based detergents are found specifically for skin disorders in Unani therapy. These drugs basically evacuate impurities from the body's excretory system and and have been found to have keratolytic and debris-peeling effects, as well as the ability to maintain skin tone consistency. Unani Jali drugs have also been found to have anti-inflammatory, antibacterial, analgesic, and tonic properties, suggesting its usefulness holistically. Based on phyto constituents, prospective therapeutic response, and scientific data, this review proposes that Unani Jali drugs could be a safe and promising therapeutic option for dermatological illnesses such as vitiligo, acne, dermatitis, psoriasis, and skin sensitivity.

Qualitative and quantitative estimation of phyto constituents in different solvent extracts of leaf of Tabernaemontana divaricata

Leaves of Tabernaemontana divaricata extracts in petroleum ether, ethanol chloroform, methanol and aqueous were analyzed for their phytochemical screening. Qualitative and quantitative phytochemical analysis was performed to identify the presence of various phytoconstituents with standard procedures. The presence of Alkaloids, Glycosides, Flavonoids, Diterpenes, Phenol, Proteins, Carbohydrate, Saponins and Tannins. For quantitative estimation of total phenols, total flavonoids, total alkaloid, total chlorophyll and total ascorbic acid were studied. The present study concluded that T. divaricata plant has the ability to cure a variety of diseases because it contents valuable phytochemicals.

QUALITATIVE ESTIMATION OF PHYTOCHEMICALS IN DIFFERENT OCIMUM SPECIES OF CHHATTISGARH

Medicinal plants and herbs have been proved to be of great importance to the health of theindividuals and communities. The potential for developing antibacterial from higher plants appearsrewarding as it will result to the development of a phytomedicine to act against microbes. Phytochemicalscreening of Tulsi plant (Ocimum sp.) was studied in the present investigation. Phytochemical analysis ofmethanol and aqueous extracts of O. tenuiflorum, O. gratissumand O. sanctum demonstrated the presenceof phytoconstituents like Tannins, Alkaloids, Saponins, Glycosides, Terpenoids, Flavonoids, Phenols,Carbohydrates, Steroids, and Anthraquinones. Presence of such phyto constituents will increase interestwith the mankind to develop anti-microbial drugs using different species of Oscimum for varied microbes.

Eco-friendly Synthesis, Spectral Characterization, Particle Image and Size Analysis of Cerium Oxide Nanoparticles Mediated by Mango Seed Aqueous Extract

A novel approach for the utilization of fruit waste is attempted in the present investigation. Mangifera indica seed aqueous extract was utilized for green synthesis of cerium oxide nanoparticles (CeONPs). The phyto constituents in the seed acted as reducing and stabilizing agent for CeONPs formation. UV-Vis, FT-IR, FL, XRD, DLS and SEM, analysis were used to characterize the green synthesized CeONPs. UV-vis spectra showed characteristic spectra at 333 nm; DLS and SEM confirmed the crystalline nature. FT-IR revealed functional groups like alcohol or phenols, carboxylic acids, ketones, amines, aromatic amines, aliphatic amines, alkyl halides and alkynes which were responsible for CeONP formation. The nanoparticles showed more CV study of cerium oxide nanoparticle. Fruit waste can be successfully utilized for cerium nanoparticles formation which can be therapeutically useful and effective.