Phytochemical Investigation and in Vitro Antioxidant Activity of Citrus Sinensis Peel Extract

Abstract

The present study was undertaken to analyse the photochemical constituents both qualitatively and quantitatively in the peel extract of Citrus sinensis. The studies showed that the major phyto constituents present in the methanolic extract was hesperidin and nobiletin. The DPPH and hydroxyl radical scavenging activity of the methanolic extract was found to be better than the other extracts used in the study. Finger printing assay of the extract was carried out using HPTLC and we report the presence of two flavonoids - nobelitin and hesperidin. Keywords: C.sinensis peel, HPTLC, antioxidant, phenolic compound, phyto constituents.