Study on phytochemical, antioxidant and analgesic activity of ethanolic extract of Curcuma Amada Roxb Rhizome

Abstract

Introduction: Curcuma amada Roxb. usually referred as ambahaldi is an exceptionally aromatic rhizomatic herb which contains number of phyto constituents responsible for several pharmacological activities. Present study is an attempt to explore antioxidant and analgesic activity of ethanolic extract of C. amada. Objectives: The goal of this work was to assess the antioxidant and analgesic activity of ethanolic extract of C. amada. Methods: For in-vitro antioxidant study, the DPPH (2, 2-Diphenyl-1-picrylhydrazyl) free radical scavenging test and nitric oxide scavenging assay was used to assess the antioxidant activity while the in-vivo analgesic efficacy was evaluated by using tail flick method and formalin induced pain in experimental animals. Different doses of 100mg/kg, 200 mg/kg, and 400 mg/kg was used to evaluate analgesic activity of ethanolic extract of C. amada. Results: Ethanolic extract of C. amada exhibited significant antioxidant in DPPH and nitric oxide scavenging assay. 100 mg/kg, 200 mg/kg, and 400 mg/kg of ethanolic extract of C. amada produced significant analgesic activity in tail flick and formalin induced pain model in experimental animals. Conclusion: Ethanolic extract of C. amada exhibited significant antioxidant as well as in-vivo analgesic activity in experimental animals.