Emblica Research Articles

Immune-Enhancing Effect of an Ayurveda formulation Vidhara-Amalaki Yog

Plant extracts and Ayurvedic polyherbal mixtures have been used to treat a variety of diseases since time immemorial. Studies on the therapeutic effects of these extracts in the treatment of various disorders are also extensively established. The synergistic effect of polyherbal medications in repairing and revitalizing the immune system is also highlighted in Ayurvedic classics. Argyreia speciosa Linn. (Elephant Creeper, Vidhara in Sanskrit; Family: Convolvulaceae) and Emblica officinalis Gaertn (Indian gooseberry, Amalaki in Sanskrit; Family: Phyllanthaceae) are important drugs explained in Ayurvedic literatures and Nighantus for their Rasayana and Bala-vardhaka properties (immunomodulatory actions). They also possess the restorative and rejuvenating powers as they act on the immune system and positively affect the response of the body towards disease causing pathogens. In this review a comprehensive account of the pharmacological activities along with the immunomodulatory activities of Vidhara- Amalaki yog (compound formulation of Vidhara and Amalaki) are included in view of the many recent findings of importance on this plant.

ROLE OF EMBLICA OFFICINALIS IN COPPER INDUCED LIVER TOXICITY IN ADULT ALBINO RATS

Background: Copper (Cu) is an important heavy metal used widely in all industries. However, its toxicity on various organs is established. Emblica officinalis, commonly known as Amla, is a potential antioxidant and can prevent Cu induced hepatotoxicity. 
 Objective: To evaluate the effects of Emblica officinalis extract on liver histology and Alanine Aminotransferase (ALT) levels after Cu induced toxicity on adult albino rats.
 Methods: This was an experimental study of 28 days duration. 36 adult male albino rats were divided into three groups of twelve animals each. Group A was control group in which rats were given 1.5 ml normal saline while group B rats were given CuSO4 200mg/kg b.w./day. Rats in Group C were given both CuSO4 (200mg/kg b.w/day) and Emblica officinalis fruit extract (300mg/kg bw/day). The agents were fed via orogastric tube for four weeks and rats were sacrificed 24 hours after administration of last dose.
 Results: Cu treated rats showed necrosis of hepatocytes and Increased serum ALT levels. Emblica officinalis co-treatment attenuated Cu-induced hepatic necrosis and variation in ALT levels. The difference among the groups was statistically significant with p-value <0.05.
 Conclusion: Emblica officinalis fruit extract improves liver function in Cu induced hepatotoxicity by limiting oxidative damage.

Search for Natural Remedies for the Treatment of Salmonella Typhi Infections

Typhoid is a major public health threat, particularly in areas lacking access to clean water, proper nutrition, and hygienic practices. The emergence of antibiotic resistance, inaccessibility of vaccines to the global south, and asymptomatic carriers of Salmonella typhi (S. typhi) have made tackling the scourge of typhoid a major challenge. Plant-based antimicrobial compounds have opened a new avenue for effectively managing typhoid by minimizing drug-related side effects and targeting the resistant strains of typhoidal salmonella cost-effectively. This review article contains many such candidates, who can potentially modify the typhoid treatment guidelines like Azadirachta indica, Canarium schweinfurthii-derived scopoletin, and Punic granatum, and those that target the resistant strains of S. typhi, like Amaranthus hybridus, and Quercus infectoria and Phyllanthus emblica. The article will also shed light on the challenges and promises associated with phytocompound extraction and standardization as the researchers navigate the uncharted waters of alternative medicinal sources.

Phyllanthus emblica L. polysaccharides ameliorate colitis via microbiota modulation and dual inhibition of the RAGE/NF-κB and MAPKs signaling pathways in rats

Pharmacological treatments for colitis have limited efficacy and side effects. Plant polysaccharides improve colitis by modulating the gut microbiota. However, the specific benefits of Phyllanthus emblica L. polysaccharides (PEPs) in colitis remain unclear. Therefore, this study aimed to assess the physical characteristics and health advantages of PEP in rats subjected to 2,4,6-trinitrobenzene sulfonic acid (TNBS) treatment. The results showed that PEP (1.226 × 103 kDa) was an α-acidic pyran heteropolysaccharide rich in galactose and galacturonic acid. Prefeeding rats with PEP significantly decreased the levels of NO, MDA, proinflammatory cytokines (IL-6, IL-1β, TNF-α), apoptosis, and the activities of mucinase and β-glucuronidase. These changes were accompanied by increases in the levels of anti-inflammatory cytokines (IL-4, IL-10) and antioxidant enzymes (SOD, catalase, GPx) in colitis rats. Mechanistically, PEP suppressed the abundance of inflammatory-related bacteria (Bacteroides, Intestinimonas, and Parabacteroides) while promoting the growth of short-chain fatty acid (SCFA)-producing bacteria (Romboutsia, Clostridium_sensu_stricto_1, and Lactobacillus), along with an increase in SCFA secretion. SCFAs may engage with the GPR43 receptor and inhibit downstream HDAC3, consequently downregulating the activation of the RAGE/NF-κB and MAPK pathways. In conclusion, PEP demonstrated preventive effects through its antioxidant, anti-inflammatory, and microbiota modulation properties, thereby ameliorating TNBS-induced colitis in rats.

Investigating the Efficacy of Herbal Preparation (Phyllanthus emblica and Curcuma longa) Powder Supplementation on Caged Broilers

Investigating the Efficacy of Herbal Preparation (Phyllanthus emblica and Curcuma longa) Powder Supplementation on Caged Broilers

Effect of Dietary Supplementation of Amla (Phyllanthus emblica, Syn. Emblica officinalis) on Growth and Health of Commercial Broiler Chicken (CARIBRO Dhanraja)

The present research work was performed at Rathindra Krishi Vigyan Kendra, Palli Siksha Bhavana (Institute of Agriculture), Visva-Bharati, Sriniketan, Birbhum, West Bengal, during February–April 2022. The objective of the present study was to explore the effect of dietary supplementation of amla (Emblica officinalis) on the health and growth of broiler chicks (CARIBRO Dhanraja). A total of thirty-six (36) one-day old chicks were randomly distributed into three dietary treatment groups with three replicates for each treatment group and four birds in each replicate to conduct the proposed study. The three experimental dietary treatments were, Control group, fed with standard diet, i.e., without amla (E. officinalis) supplementation (T1), Test groups, fed with 1.5% amla (E. officinalis) supplemented diet (T2) and 2.5% amla (E. officinalis) supplemented diet (T3). From the results, the highest average body weight, highest weekly average body weight gain and better Feed Conversion Ratio were observed in T2 followed by T3. It was found that serum SGPT/ALT, SGOT/AST and ALP levels were found to be highest in the control group (T1), and were significantly reduced in amla-supplemented groups, least levels were observed in the T3 group. There was no significant difference observed in dressing yield among the treatments. However, in some carcass parameters, a significant difference was observed among the groups with different treatments. From the above study, it could be concluded that feed supplemented with amla (E. officinalis) improved the health and growth of the broiler chicken, which is economically profitable to the poultry farmers.

Research Article Development and Evaluation of Antidandruff Shampoo Based on Herbal Source

Shampoo are a common cosmetic item used in daily life. It is the cosmetic preparation intended to clean the hair by removing the dirt from the hair shaft and scalp. In the current outline it seems unlikely that herbal shampoo although better performing and safer than synthetic shampoo. Synthetic shampoo shows harmful effect on the hair and scalp such as hair dryness and keratin loss for this reason the herbal shampoo has been developed. The formula of herbal shampoo using extract such as Sapindus mokoros , Emblica officinalis, Acacia concinna , Ocimum sanctum , Eclipta prostrata , Trigonella foenum graecum in different proportions. The evaluation test has been performed like organoleptic, wetting time test, physicochemical pH, detergency, dirt dispersion, stability have been accomplished. The main aim of this study was to remove harmful synthetic ingredient from antidandruff shampoo formulation and replace them with natural ingredients.

A Comparative Analysis for Phytochemical Screening and Antioxidant Potential of Two Different Formulations of <i>Triphala</i> Extract

In Ayurveda, Triphala is a formulation of three herbs. It is a mixture (equiproportional 1:1:1) of fruits of three plants, Haritaki (Terminalia chebula), Bibhitaki (Terminalia bellerica) and Amalaki (Emblica officinalis). Triphala shows antibacterial, antiallergic, antiviral, antifungal, immunomodulatory and antioxidant properties. Triphala and its constituents help in the improvement of blood circulation, reduction in level of cholesterol and control of blood pressure. This study focuses on detecting various phytocompounds and their comparative analysis for antioxidant potential in two different formulations of Triphala extract. Antioxidant activity was determined by phosphomolybdenum assay and reducing power assay. Our results indicated the presence of various phytocompounds such as phenols, flavonoids, tannins, saponins, alkaloids etc, which may play a role in its biological activities. Both Triphala formulations showed significant free radical-scavenging properties as compared to ascorbic acid. Formulation S2 (H:B:A - 1:2:3) showed higher antioxidant activity as compared to formulation S1 (H:B:A- 1:1:1). In conclusion, Triphala non-equiproportional mixture may be a more promising Ayurvedic drug in future Triphala.

Comparative physiological and transcriptome analysis provide insights into the inhibitory effect of osthole on Penicillium choerospondiatis

Blue mold induced by Penicillium choerospondiatis is a primary cause of growth and postharvest losses in the fruit of Phyllanthus emblica. There is an urgent need to explore novel and safe fungicides to control this disease. Here, we demonstrated osthole, a natural coumarin compound isolated from Cnidium monnieri, exhibited a strong inhibitory effect on mycelia growth, conidial germination rate and germ tube length of P. choerospondiatis, and effectively suppressed the blue mold development in postharvest fruit of P. emblica. The median effective concentration of osthole was 9.86 mg/L. Osthole treatment resulted in cellular structural disruption, reactive oxygen species (ROS) accumulation, and induced autophagic vacuoles containing cytoplasmic components in fungal cells. Transcriptome analysis revealed that osthole treatment led to the differentially expressed genes mainly enriched in the cell wall synthesis, TCA cycle, glycolysis/ gluconeogenesis, oxidative phosphorylation. Moreover, osthole treatment led to increase genes expression involved in peroxisome, autophagy and endocytosis. Particularly, the autophagy pathway related genes (PcATG1, PcATG3, PcATG15, PcATG27, PcYPT7 and PcSEC18) were prominently up-regulated by osthole. Summarily, these results revealed the potential antifungal mechanism of osthole against P. choerospondiatis. Osthole has potentials to develop as a natural antifungal agent for controlling blue mold disease in postharvest fruits.

Folk medicinal plants in forest fringe villages of tribal’s hill districts of Nagaland, India

In forest fringe villages, folk medicines play a pivotal role in human health care management. The aim of present study was to document the plants employed in the folk medicines by ethnic groups inhabiting forest fringe villages of Nagaland. Field survey was conducted for over two years in 32 forest fringe villages of Nagaland. The data were collected through semi-structured interviews and quantitatively analyzed by statistical tools i.e., Use-value, Relative frequency of citation and Informant consensus factor to identify promising plants for future studies. This research documented 96 medicinal plants employed in the treatment of 59 ailments of human being. The quantitative analysis of the data indicates that some species have relatively high ―use value (UV)‖ and ―relative frequency of citation (Rfc)‖, these are: Azadirachta indica A. Juss., Phyllanthus emblica L. with 0.67 as UV and 3.6 as Rfc. Maximum consensus among informants was observed for the treatment of tooth and gum ailment and under this category Solanum aculeatissimum Moench was frequently cited by informants. Those plants which receive high relative frequency of citation values may serve as potential source for pharmacological studies, especially Azadirachta indica A. Juss. (used as insect repellent), Phyllanthus emblica L. (used in abdominal pain and indigestion), Alstonia scholaris (L.) R.Br. (in flatulence), Aloe vera Mill. (in constipation), Canna indica L. (in genital infection), Gmelina arborea Roxb. ex Sm. (prevent miscarriage), Parkia timoriana (DC.) Merr. (in bleeding piles), Carica papaya (DC.) Merr. (cure ringworm), Solanum aculeatissimum Moench (in foot and toe infection), Thalictrum foliolosum DC. (for vomiting and indigestion), Mentha spicata L. (in stomach ache), Elettaria cardamomum (L.) Maton (incough and cold), Zingiber officinale Roscoe (in cough and cold) and Persicaria hydropiper (L.) Delarbre (in skin infection).

Effects of Piper betle and Phyllanthus emblica leaf extracts on the growth and resistance of black tiger shrimp, Penaeus monodon, against pathogenic Vibrio parahaemolyticus

Effects of Piper betle and Phyllanthus emblica leaf extracts on the growth and resistance of black tiger shrimp, Penaeus monodon, against pathogenic Vibrio parahaemolyticus

Facile green synthesis of Phyllanthus emblica extract based Ag-NPs for antimicrobial and response surface methodology based catalytic reduction applications

Silver nanostructures were synthesized by utilizing facile and simple green methodology based on the usage of biogenic Phyllanthus emblica fruit extract. The Fourier transform infrared spectroscopy revealed that the numerous phytochemicals were found to be present in the extract that were responsible for reducing the precursor salt into nanoparticles. After 10 min, the silver nanoparticles were formed in the system while the further nucleation for 30 min resulted in the generation of the silver nanostructures with dual morphology (spherical & rod-like morphology). The reaction time/nucleation time was found to be the major factor responsible for the modulation of the morphology of the biogenic nanomaterials. The crystalline nature with the cubic crystal system was observed in the case of the silver nanostructure via X-ray diffraction analysis. The antimicrobial efficacy investigated for these nanostructures revealed that the silver nanostructures possess comparable or greater efficacy than the selected control. The excellent efficacy is attributed to the smaller size and the peculiar morphological characteristics of the silver nanostructures. The catalytic reduction of 4-nitrophenol via silver nanostructures was also investigated by using the response surface methodology as an optimization tool. An extremely high percentage reduction value of 96.491 % with the rate constant value of 0.277 min−1 was documented in the case of Ag-NSs. The prepared silver nanostructures were found to be extremely stable till six reuse cycles. Our findings affirm that the efficient synthesis of nanostructures is achieved by using P. emblica fruit extract and the synthesized material further presents excellent viability for antimicrobial and catalytic applications.

Advancement in Tissue Culture Techniques for Fruit Crops

Tissue culture is a highly promising approach that enables the efficient propagation of many plants from tiny fragments of the parent plant within a relatively brief timeframe and confined area. Tissue culture, a contemporary approach, is primarily employed for the efficient and extensive replication of many commercially significant plant species, such as the date palm. Utilizing the tissue culture technique presents a potential approach for generating a substantial quantity of genetically homogeneous palm plants that resemble other plants and yield typical fruit within four years from initial planting. Furthermore, this technique allows to produce date palm plants devoid of diseases, exhibiting an exceptionally high survival rate of nearly 100% when compared to the traditional vegetative propagation of shoots, owing to the robustness of their root system. The process of surface sterilization holds significant importance in the production of explants for in vitro studies, as it effectively addresses the issue of bacterial and fungal contamination originating from field sources, which might vary considerably across different fruit plant species. The efficacy of tissue culture techniques for date palm acclimatization in vitro is contingent upon the observation of leaf count prior to transplantation in the greenhouse. Hence, the primary objective of this study was to investigate the determinants that govern the tissue culture of fruit trees. India is known for being the native land of various fruit crops that are both significant and minor in terms of their importance. These crops include Indian gooseberry (Emblica officinalis Gaertn.), Karonda (Carissa carandas L.), Bael (Aegle marmelos Corr.), Jamun (Syzygium cuminii L.), and jackfruit (Artocarpus heterophyllus L.), etc. These fruits possess considerable nutritional, medicinal, and therapeutic value, making them highly valuable in commercial sectors such as medicine, food, and cosmetics. The limited availability of suitable planting materials imposes constraints on the commercial production process for these crops. Using plant tissue culture techniques holds promise in substantially augmenting the number of novel cultivars or genotypes inside fruit crops. The primary aim of this review study is to consolidate and synthesize the extant body of knowledge about the tissue culture techniques employed in cultivating various fruit crops.

Circulatory GSK‐3beta a new target against Alzheimer’s disease

AbstractBackgroundAlzheimer’s disease (AD) is the progressive brain disorder which degenerate brain cells connection and causes memory loss. Though, AD is irreversible, it is not impossible to arrest or slow down the progression of the disease. However, this would only be possible if the disease is diagnosed at an early stage and early diagnosis requires clear understanding of pathogenesis at molecular level. Overactivity of GSK‐3β and p53 accounts for tau hyperphosphorylation, and amyloid‐β plaqueMethodWe explored GSK‐3β and p53 as blood‐based biomarkers for early detection of AD. Methods: The levels of GSK‐3β, p53, and their phosphorylated states were measured using surface plasmon resonance and verified using Western blot in serum from AD, mild cognitive impairment (MCI), and geriatric‐control (GC) subjects. The neurotoxic SH‐SY5Y cell line was treated with antioxidant Emblica Officinalis (EO) for rescue effect.ResultGSK‐3β, p53, and their phosphorylated state were significantly over expressed (p>0.001) in AD, MCI compared to GC and can differentiate AD, MCI from GC. The expression level of GSK‐3β and p53 proteins were found to be downregulated in a dose‐dependent manner after the treatment with EO in amyloid‐β induced neurotoxic cells.ConclusionThese proteins can serve as potential blood markers for the diagnosis of AD and EO can suppress their level. This work has translational value and clinical utility in the future.

Effect of particle size on the phenolic and flavonoid contents of Amla (Indian gooseberry) extract

Amla (Indian gooseberry) is a traditional medicinal plant that contains phenolic and flavonoid compounds which can treat gastric ailments, diabetic, as an antioxidant and antimicrobial. This study investigated the effects of particle size on the yield, phenolic and flavonoid contents of amla extract. Fresh amla was oven-dried and ground into powder to obtain samples with different particle sizes. Extraction of phenolic and flavonoid contents on amla was carried out by maceration method using ethanol 96%. The phenolic and flavonoid contents of amla extract were analyzed quantitatively using spectrophotometry UV-Vis. The highest yield (31.43%) of amla extract was found at particle sizes between 0.250-0.149 mm. The results appeared that diverse particle size had a major impact on phenolic and flavonoid content. The highest phenolic content of 289.365 mg GAE/g and flavonoid content of 7.476 mg QE/g were obtained at particle sizes between 0.250-0.149 mm.

Survival Status of Young Plants in Paluwatar Plantation Site of Udayapur District, Nepal

Forests provide multitude of benefits, including carbon sequestration, biodiversity conservation, timber production, and recreational opportunities. However, these valuable ecosystems are facing severe threats due to deforestation and land degradation. To counter this, plantation has emerged as a key strategy to restore degraded lands and ensure a continuous supply of ecosystem services. Yet, the success of these plantation sites remains uncertain due to a lack of systematic assessment of their survival status. Therefore, this study focuses on evaluating the survival status of young plantations on degraded riverbanks in Udayapur District, Nepal. Through systematic sampling at 0.5% intensity, 30 plots, each spanning 25 m², were established for regeneration inventory. Soil samples were collected using soil augers to a depth of 30cm for nutrient analysis. Nitrogen (N), Phosphorus (P), and Potassium (K) levels were determined using soil testing kits, while organic matter (OM) content was assessed using the Loss-on-Ignition (LOI) method. The results revealed an encouraging overall seedling survival rate of 95% at the research site. Notably, Dalbergia sissoo (Sissoo) and Tectona grandis (Teak) exhibited the highest survival rates at 100%, whereas Phyllanthus emblica (Amala) and Delonix regia (Gulmohar) showed lower survival rates at 40%. The site demonstrated low levels of N, P, and K, alongside high organic matter content. Unexpected high survival rate (95%) despite presence of a low level of nutrients raises intriguing questions about the adaptive mechanisms of the planted tree species. Further replication of similar studies as well as investigation of factors playing crucial role in seedling survival and growth beyond soil nutrient status is recommended.

Evaluation The Ameliorative Effect of Alcoholic Extracts of Citrullus colocynthis and Phyllanthus Emblica Fruits on Streptozotocin-Induced Diabetes in Male Rats

Evaluation The Ameliorative Effect of Alcoholic Extracts of Citrullus colocynthis and Phyllanthus Emblica Fruits on Streptozotocin-Induced Diabetes in Male Rats

Green Synthesis of Silver Nanoparticles from Phyllanthus emblica (Indian Gooseberry) for Potent Antimicrobial Efficacy

Nowadays cost-effective and environmentally friendly technologies for nanomaterial synthesis have gained attention in the biosynthesis of nanoparticles. The present study is focused on synthesizing silver nanoparticles using Phyllanthus emblica for a green approach. UV-Vis Spectrophotometry, XRD, SEM, and FTIR were used to characterise the synthesised silver nanoparticles. It was determined that spherical shaped nanoparticles by SEM examination, which revealed that the average particle size was 20 nm. Antibacterial activity of AgNPs analyzed with the help of Staphylococcus aureus, Vibrio cholerae, Pseudomonas auriginosa, and Bacillus subtilis bacteria, and the result shows that a higher concentration of AgNPs is increasing as well as a zone of inhibition increased.

The antihyperlipidemic effect of a combined supplement of standardized dry extracts of amla (Emblica officinalis), walnut (Juglans regia), olive (Olea europaea) and red yeast rice (Monascus purpureus) powder: Reduction in circulatory low-density lipoprotein-cholesterol (LDL-C) and remnant cholesterol (RC) levels in patients with hypercholesterolemia

Background: Hyperlipidemia is associated with a higher rate of cardiovascular, cerebrovascular, and peripheral vascular disease. Conventional drugs such as statins are effective in controlling hyperlipidemia; however, they are associated with various side effects, especially myalgia. Nutraceutical lipid-lowering interventions are becoming increasingly popular, particularly among patients who are intolerant or refractory to statins. Substantial preclinical and clinical evidence suggests that extracts of amla, walnut, and olive, and red yeast rice (RYR) powder possess significant antihyperlipidemic effects.Aims: This study aimed to evaluate the efficacy, safety, and patient satisfaction of a combined supplementation of standardized dry extracts of amla fruit (500 mg), walnut leaves (50 mg), olive fruit (25 mg), and RYR powder (33.6 mg) (Cholesfytol NG®) in hypercholesterolemic patients.Methods: This was a real-life setting, retrospective, observational, single-arm, non-randomized study in hypercholesterolemic patients (total cholesterol (TC) ≥ 200 mg/dL or low-density lipoprotein-cholesterol (LDL-C) ≥ 130 mg/dL), enrolled at 57 general practitioner (GP) surgeries in Belgium from March 2020 to January 2022. These patients received a GP-prescribed daily single dosage of two oral tablets of Cholesfytol NG® supplementation for 2 months to overcome their hypercholesterolemia in the absence of a conventional lipid-lowering drug (n = 208) or with a lipid-lowering drug (n = 13). At 2-month follow-up, the lipid profile was re-evaluated, alongside a patient’s questionnaire on treatment general satisfaction and willingness to pursue supplementation.Results: After supplementation, TC decreased by 15%, LDL-C by 19%, non-high-density lipoprotein-cholesterol (non-HDL-C) by 20% (all p < 0.0001), triglycerides (TG) by 9% (p = 0.0028) (−18.4%, p = 0.0042, in patients with baseline TG > 180 mg/dL, n = 58), and remnant cholesterol (RC) by 12% (p = 0.0001). These changes were unaffected by statin intolerance status in patients who received Cholesfytol NG® alongside statin. The supplement was well tolerated by all patients, and no serious adverse events or supplement-emergent effects were reported. Most patients were satisfied with the supplementation and wanted to pursue the nutraceutical.Conclusion: According to the results of this study, a combined supplementation of amla, walnut, and olive extracts, and RYR powder exerts a significant antihyperlipidemic effect, leading to a decrease in circulatory LDL-C and RC levels in patients with hypercholesterolemia. The supplementation bears excellent safety and tolerability, and is rated as satisfactory and pursuable, even among patients with statin intolerance.Clinical Trial Registration: clinicaltrials.gov; identifier number: NCT06002893

Comparative study of Nishaamalaki and metformin in obese patients of type 2 diabetes mellitus (Madhumeha): A study protocol

Background Poor synthesis of insulin by beta cells present in the pancreas combined with resistance of insulin in peripheral organs is referred to as type 2 diabetes mellitus. Insulin resistance leads to an increase in plasma fatty acids, which reduces transfer of glucose within the cells of muscles and increases breakage of lipids, resulting in an increase in hepatic glucose production. Nishamalaki (a formulation of turmeric and Indian gooseberry) is suggested in the therapy of all kinds of Madhumeha (diabetes mellitus) in Ayurveda classics. Turmeric and Indian gooseberry are the two main ingredients. Both are considered as effective medicines in the management of Madhumeha individually as well as in combined form. Aim To compare the efficacy of Nishaamalaki and metformin in obese patients of type2 diabetes mellitus. Methods The study will include 60 obese type 2 diabetes mellitus patients who were distributed into two distinct categories, each with 30 patients. Nishamalaki Churna 3gm two times a day before food with warm water for 60 days in Group N (Experimental Group) and 500 mg metformin tablets twice daily before meals for 60 days in Group M (Control Group). Every 15th day, an assessment was made (15th, 30th, 45th and 60th day). Results Objective outcomes will be assessed. Conclusion: From this study protocol, it can be depicted that the trial formulation of turmeric and Indian gooseberry (Nisha-amalaki) is as effective in obese patients suffering from type- 2 diabetes mellitus as compared to metformin tablets.