Photothermal Activity Research Articles

Hydrogen-bonding induced assembly of polymer-grafted nanoparticles towards photothermal antibacterial activities

The multiple hydrogen-bond has been introduced as a reversible driving force for directing the assembly of polymer-grafted nanoparticles (PGNPs). The complementary hydrogen-bonds among the polymer ligands lead to the spontaneous aggregation of PGNPs. However, it may also induce the uncontrollable aggregation of PGNPs into assemblies with non-uniform size, even irregular precipitates, due to the immoderate agglomeration associated with the strong interactions of multiple hydrogen-bonds. This severely limits the stable dispersion of PGNP aggregates in a solvent and their applications. In this work, the gold nanoparticles (AuNPs) grafted with thymine-terminated polystyrene (AuNP@PS-Thy) and diaminopyridine-terminated polystyrene (AuNP@PS-Dap) were synthesized, respectively. Their thermal-responsive assembly behavior in an organic solvent was systematically studied. By optimizing the assembly conditions, i.e., the concentration of PGNPs and the incubation time, the assemblies of AuNP@PS-Thy/AuNP@PS-Dap with controllable size were obtained. Interestingly, the assemblies deposited on a solid substrate showed excellent photothermal antimicrobial activities under irradiation of 808 nm and 655 nm lasers. The killing percentage of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) could reach 99 % after irradiating for 10 min. This work establishes an approach for controlling the hydrogen-bonding-induced assembly behavior of PGNPs, which may be extended to construct functional metamaterials with controllable structures.

Investigation of the photothermal properties of a large series of metal-bis(dithiolene) complexes: Impact of the molecular structure and ranking using the photothermal index IPT

The photothermal properties in the NIR region of a large series of 19 metal-bis(dithiolene) complexes and of a perylene derivative have been studied in detail. Photophysical and thermal measurements were repeated several times to obtain good uncertainties on molar extinction coefficients, temperature increases and photothermal efficiencies. This large series of metal-bis(dithiolene) complexes enabled us to assess the impact of the nature of the metal center, the nature of the ligand and the number and length of the carbon chains on absorption properties and photothermal efficiencies. Thanks to these reliable data, the recently introduced photothermal index IPT, which combines molar absorptivity ε and photothermal efficiency η, has also been effectively used to rank all these molecular photothermal agents according to their photothermal activity. This work confirms that the photothermal index IPT can be used effectively to rank photothermal agents according to their ability to convert light into heat.

Hollow Cu2-xS@NiFe Layered Double Hydroxide Core-Shell S-Scheme Heterojunctions with Broad-Spectrum Response and Enhanced Photothermal-Photocatalytic Performance.

Designing a reasonable heterojunction is an efficient path to improve the separation of photogenerated charges and enhance photocatalytic activity. In this study, Cu2-xS@NiFe-LDH hollow nanoboxes with core-shell structure are successfully prepared. The results show that Cu2-xS@NiFe-LDH with broad-spectrum response has good photothermal and photocatalytic activity, and the photocatalytic activity and stability of the catalyst are enhanced by the establishment of unique hollow structure and core-shell heterojunction structure. Transient PL spectra (TRPL) indicates that constructing Cu2-xS@NiFe-LDH heterojunction can prolong carrier lifetime obviously. Cu2-xS@NiFe-LDH shows a high photocatalytic hydrogen production efficiency (5176.93µmolh-1g-1), and tetracycline degradation efficiency (98.3%), and its hydrogen production rate is ≈10-12 times that of pure Cu2-xS and NiFe-LDH. In situ X-ray photoelectron spectroscopy (XPS) and electron spin resonance (ESR) provide proofs of the S-scheme electron transfer path. The S-scheme heterojunction achieves high spatial charge separation and exhibits strong photoredox ability, thus improving the photocatalytic performance.

Synchronized Chemotherapy/Photothermal Therapy/Sonodynamic Therapy of Human Triple-Negative and Estrogen Receptor-Positive Breast Cancer Cells Using a Doxorubicin–Gold Nanoclusters–Albumin Nanobioconjugate

ObjectiveNovel strategies for treating triple-negative breast cancer (TNBC) are ongoing because of the lack of standard-of-care treatment. Nanoframed materials with a protein pillar are considered a valuable tool for designing multigoals of energy-absorbing/medication cargo and are a bridge to cross-conventional treatment strategies. MethodsNanobioconjugates of gold nanoclusters–bovine serum albumin (AuNCs–BSA) and doxorubicin–AuNCs–BSA (Dox–AuNCs–BSA) were prepared and employed as a simultaneous double photosensitizer/sonosensitizer and triple chemotherapeutic/photosensitizer/sonosensitizer, respectively. ResultsThe highly stable AuNCs–BSA and Dox–AuNCs–BSA have ζ potentials of –29 and –18 mV, respectively, and represent valuable photothermal and sonodynamic activities for the combination of photothermal therapy and sonodynamic therapy (PTT/SDT) and synchronized chemotherapy/photothermal therapy/sonodynamic therapy (CTX/PTT/SDT) of human TNBC cells, respectively. The efficiency of photothermal conversion of AuNCs–BSA was calculated to be a promising value of 32.9%. AuNCs–BSA and Dox–AuNCs–BSA were activated on either laser light irradiation or ultrasound exposure with the highest efficiency on the combination of both types of radiation. CTX/PTT/SDT of MCF-7 and MDA-MB-231 breast cancer cell lines by Dox–AuNCs–BSA were evaluated with the MTT cell proliferation assay and found to progress synergistically. ConclusionResults of the MTT assay, detection of the generation of intracellular reactive oxygen species and occurrence of apoptosis in the cells confirmed that CTX/PTT/SDT by Dox–AuNCs–BSA was attained with lower needed doses of the drug and improved tumor cell ablation, which would result in the enhancement of therapeutic efficacy and overcoming of therapeutic resistance.

Molecular Photothermal Conversion Catalyst Promotes Photocontrolled Atom Transfer Radical Polymerization.

Photothermal conversion is a growing research area that promotes thermal transformations with visible light irradiation. However, few examples of dual photothermal conversion and catalysis limit the power of this phenomenon. Here, we take inspiration from nature's ability to use porphyrinic compounds for nonradiative relaxation to convert light into heat to facilitate thermal polymerization catalysis. We identify the photothermal conversion catalytic activity of a vitamin B12 derivative, heptamethyl ester cobyrinate (HME-Cob), to perform atom transfer radical polymerization (ATRP) under irradiation. Rapid polymerization are obtained under photothermal activation while maintaining good control over polymerization with the aid of a photoinitiator to enable light-induced catalyst regeneration. The catalyst exhibits exquisite temporal control in photocontrolled thermal polymerization. Ultimately, the activation of this complex is accessed across a broad range of wavelengths, including near-IR light, with excellent temporal control. This work showcases the potential of developing photothermal conversion catalysts.

Nano-Innovations in Cancer Therapy: The Unparalleled Potential of MXene Conjugates.

MXenes are two-dimensional transition metal carbides, nitrides, and carbonitrides that have become important materials in nanotechnology because of their remarkable mechanical, electrical, and thermal characteristics. This review emphasizes how crucial MXene conjugates are for several biomedical applications, especially in the field of cancer. These two-dimensional (2D) nanoconjugates with photothermal, chemotherapeutic, and photodynamic activities have demonstrated promise for highly effective and noninvasive anticancer therapy. MXene conjugates, with their distinctive optical capabilities, have been employed for bioimaging and biosensing, and their excellent light-to-heat conversion efficiency makes them perfect biocompatible and notably proficient nanoscale agents for photothermal applications. The synthesis and characterization of MXenes provide a framework for an in-depth understanding of various fabrication techniques and their importance in the customized formation of MXene conjugates. The following sections explore MXene-based conjugates for nanotheranostics and demonstrate their enormous potential for biomedical applications. Nanoconjugates, such as polymers, metals, graphene, hydrogels, biomimetics, quantum dots, and radio conjugates, exhibit unique properties that can be used for various therapeutic and diagnostic applications in the field of cancer nanotheranostics. An additional layer of understanding into the safety concerns of MXene nanoconjugates is provided by detailing their toxicity viewpoints. Furthermore, the review concludes by addressing the opportunities and challenges in the clinical translation of MXene-based nanoconjugates, emphasizing their potential in real-world medical practices.

Polysaccharide hydrogel containing silver nanoparticle@catechol microspheres with photothermal, antibacterial and anti-inflammatory activities for infected-wounds repair

Anti-infection hydrogels have recently aroused enormous attraction, particularly in the treatment of chronic wounds. Herein, silver nanoparticle@catechol formaldehyde resin microspheres (Ag@CFRs) were fabricated by one-step hydrothermal method and subsequently encapsulated in hydrogels which were developed by Schiff base reaction between aldehyde groups in oxidized hyaluronic acid and amino groups in carboxymethyl chitosan. The developed polysaccharide hydrogel exhibited microporous structure, high swelling capacity, favorable mechanical strength, enhanced tissue adhesion and photothermal activities. Additionally, the hydrogel not only ensured long-term and high-efficiency antibacterial performance (99.9 %) toward E. coli and S. aureus, but also realized superior cytocompatibility in vitro. Moreover, based on the triple antibacterial strategies endowed by chitosan, silver nanoparticles and the photothermal properties of catechol microspheres, the composite hydrogel exhibited excellent anti-infection function, significantly downregulated inflammatory factors (TNF-α and IL-1β) and promoted in vivo infected-wound healing. These results demonstrated that the polysaccharide hydrogel containing Ag@CFRs has great potential for infected-wounds repair.

In-situ exsolved ultrafine Ni nanoparticles from CeZrNiO2 solid solution for efficient photothermal catalytic CO2 reduction by CH4

CO2 reduction by CH4 (CRM) to produce fuel is of great significance for solar energy storage and eliminating greenhouse gas. Herein, the catalyst of ultrafine Ni nanoparticles supported on CeZrNiO2 solid solution (Ni@CZNO) was synthesized by the sol-gel method. High yield of H2 and CO (58.0 and 69.8 ​mmol ​min−1 ​g−1) and excellent durability (50 ​h) were achieved by photothermal catalytic CRM merely under focused light irradiation. Structural characterization and DFT calculations reveal that CZNO has rich oxygen vacancies that can adsorb and activate CO2 to produce reactive oxygen species. Oxygen species are transferred to ultrafine Ni nanoparticles through the rich Ni-CZNO interface to accelerate carbon oxidation, thereby maintaining the excellent catalytic stability of the catalyst. Moreover, the experimental results reveal that light irradiation can not only enhance the photothermal catalytic CRM activity through photothermal conversion and molecular activation, but also improve the stability by increasing the concentration of oxygen vacancies and inhibiting CO disproportionation.

Construction of Interlayer Coupling Diatomic Nanozyme with Peroxidase-Like and Photothermal Activities for Efficient Synergistic Antibacteria.

Pathogenic bacteria are the main cause of bacterial infectious diseases, which have posed a grave threat to public health. Single-atom nanozymes have emerged as promising candidates for antibacterial applications, but their activities need to be further improved. Considering diatomic nanozymes exhibit superior metal loading capacities and enhanced catalytic performance, a new interlayer coupling diatomic nanozyme (IC-DAN) is constructed by modulating the coordination environment in an atomic-level engineering. It is well demonstrated that IC-DAN exhibited superior peroxidase-mimetic activity in the presence of H2O2 to yield abundant ∙OH and possessed high photothermal conversion ability, which synergistically achieves efficient antibacterial therapy. Therefore, IC-DAN shows great potential used as antibacterial agent in clinic and this study open a new route to developing high-performance artificial enzymes.

Vinorelbine-loaded multifunctional magnetic nanoparticles as anticancer drug delivery systems: synthesis, characterization, and in vitro release study.

In this study, a multifunctional therapeutic agent combining chemotherapy and photothermal therapy on a single platform has been developed in the form of vinorelbine-loaded polydopamine-coated iron oxide nanoparticles. Vinorelbine (VNB) is loaded on the surface of iron oxide nanoparticles produced by a solvothermal technique after coating with polydopamine (PDA) with varying weight ratios as a result of dopamine polymerisation and covalent bonding of thiol-polyethylene glycol (SH-PEG). The VNB/PDA/Fe3O4 nanoparticles have a saturation magnetisation value of 60.40 emu/g in vibrating sample magnetometry, which proves their magnetisation. Vinorelbine, which is used as an effective cancer therapy agent, is included in the nanocomposite structure, and in vitro drug release studies under different pH conditions (pH 5.5 and 7.4) and photothermal activity at 808 nm NIR laser irradiation are investigated. The comprehensive integration of precise multifunctional nanoparticles design, magnetic response, and controlled drug release with photothermal effect brings a different perspective to advanced cancer treatment research.

In-situ surface bismuth assembled amorphous BiOI nanoplatforms for enhancing NIR-triggered bacterial inactivation

A large number of antibiotics show inadequate antibacterial activity and lead to the emergence of resistant-drug bacteria. Thus, exploring new antibacterial agents with antibiotics-free is a crucial task for decreasing further environmental and health risks. In this work, an amorphous BiOI nanoparticle with in-situ nanoscale bismuth growth and oxygen vacancy construction (BiOI@Bi-V) is rationally designed to perform an high-performance antibacterial activity. Benefits from the reasonable heterojunction and amorphous type, BiOI@Bi-V with an enhanced heterojunction-assistant internal electric field can improves electron transfer to effectively promote the separation of carriers and holes, further heightening photocatalytic activity. Meanwhile, the satisfactory photothermal ability is also driven by tuning crystal structural integrity and surface Bi growth, the photothermal effect synergistically develops the catalytic activity through a rapid molecular thermal motion reaction. Ultimately, the prepared BiOI@Bi-V achieves satisfactory photo-active antibacterial capability by employing the synergistic advantages of photothermal and photocatalytic activity. This work provides a promising consultation for designing highly efficient hybrid NIR-driven bactericidal agents by using an amorphous and in-situ surface growth strategy of nanoparticles.

Layered High-Entropy Metallic Glasses for Photothermal CO2 Methanation.

High entropy alloys and metallic glasses, as two typical metastable nanomaterials, have attracted tremendous interest in energy conversion catalysis due to their high reactivity in nonequilibrium states. Herein, a novel nanomaterial, layered high entropy metallic glass (HEMG), in a higher energy state than low-entropy alloys and its crystalline counterpart due to both the disordered elemental and structural arrangements, is synthesized. Specifically, the MnNiZrRuCe HEMG exhibits highly enhanced photothermal catalytic activity and long-term stability. An unprecedented CO2 methanation rate of 489mmol g-1 h-1 at 330°C is achieved, which is, to the authors' knowledge, the highest photothermal CO2 methanation rate in flow reactors. The remarkable activity originates from the abundant free volume and high internal energy state of HEMG, which lead to the extraordinary heterolytic H2 dissociation capacity. The high-entropy effect also ensures the excellent stability of HEMG for up to 450 h. This work not only provides a new perspective on the catalytic mechanism of HEMG, but also sheds light on the great catalytic potential in future carbon-negative industry.

Crown-ether threaded covalent organic polyrotaxane framework (COPF) towards synergistic crown/Zn2+/photothermal/photodynamic antibacterial and infected wound healing therapy

Controllable preparation of materials with new structure has always been the top priority of polymer materials science research. Here, the supramolecular binding strategy is adopted to develop covalent organic frameworks (COFs) with novel structures and functions. Based on this, a two-dimensional crown-ether ring threaded covalent organic framework (COF), denoted as Crown-COPF with intrinsic photothermal (PTT) and photodynamic (PDT) therapeutic capacity, was facilely developed using crown-ether threaded rotaxane and porphyrin as building blocks. Crown-COPF with discrete mechanically interlocked blocks in the open pore could be used as a molecular machine, in which crown-ether served as the wheel sliding along the axle under the laser stimulation. As a result, Crown-COPF combining with the bactericidal power of crown ether displayed a significant photothermal and photodynamic antibacterial activity towards both the Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus), far exceeding the traditional Crown-free COF. Noteworthily, the bactericidal performance could be further enhanced via impregnation of Zn2+ ions (Crown-COPF-Zn) flexible coordinated with the multiple coordination sites (crown-ether, bipyridine, and porphyrin), which not only endow the positive charge with the skeleton, enhancing its ability to bind to the bacterial membrane, but also introduce the bactericidal ability of zinc ions. Notably, in vivo experiments on mice with back infections indicates Crown-COPF-Zn with self-adaptive multinuclear zinc center, could effectively promote the repairing of wounds. This study paves a new avenue for the effectively preparation of porous polymers with brand new structure, which provides opportunities for COF and mechanically interlocked polymers (MIPs) research and applications.

Interventional Hydrogel Microsphere Controlled‐Releasing Curcumin for Photothermal Therapy against Endometriosis

AbstractEndometriosis is a prevalent gynecological disorder characterized by the presence of endometrial‐like tissue outside the uterus, causing chronic pelvic pain, painful periods, and infertility. Despite the common use of hormone therapy and surgical lesion excision in its treatment, there remain significant postoperative risks and potential side effects, with a high recurrence rate. An efficient nonsurgical treatment for this condition is therefore urgently needed. In this study, an interventional hydrogel microsphere with near‐infrared (NIR)‐controlled curcumin release is developed for the non‐invasive thermal therapy of endometriosis. The hydrogel microspheres demonstrate good biocompatibility and photothermal activities, causing the death of endometrial stromal cells under NIR and controlled curcumin (Cur) release. When injected into endometriotic lesions and stimulated by NIR light, the interventional hydrogel microspheres (Cur‐FeHMPs) can raise the temperature of the endometriotic lesions to 43.65 °C, inducing endometriotic lesion elimination. Furthermore, the controlled curcumin release from the interventional hydrogel inhibits the proinflammatory environment in endometriosis. The Cur‐FeHMPs in combination with NIR present a novel strategy for endometriosis treatment.

Photothermal/photodynamic antibacterial hydrogel embedded with copper carbon dots and Au nanoparticles

In this paper, we created a hydrogel (named AuNPs-CuCCDs@Gel) with photothermal and photodynamic performance through incorporating copper carbon dots (CuCCDs) and Au nanoparticles (AuNPs) into gelatin and chitosan hydrogels. The thus-obtained hydrogel exhibited a high photothermal and photodynamic antibacterial activity with a superior singlet oxygen yield (0.70), which was better than that of methylene blue (0.52). After laser irradiation at 808 nm for 6 min, the temperature of AuNPs-CuCCDs@Gel rose from room temperature to 50 °C, which manifested good effects for avoiding local heat damage and protecting normal cells. AuNPs-CuCCDs@Gel exhibited the highest antibacterial efficacy of 96.46% and 97.48% against S. aureus and E. coli respectively. The significantly improved bactericidal performance was attributed to the synergistic effects of hyperthermia and reactive oxygen species (ROS). This work suggests that photothermal/photodynamic antibacterial hydrogel is a promising agent for treating wound infection.

Regenerated silk fibroin coating stable liquid metal nanoparticles enhance photothermal antimicrobial activity of hydrogel for wound infection repair

The integration of liquid metal (LM) and regenerated silk fibroin (RSF) hydrogel holds great potential for achieving effective antibacterial wound treatment through the LM photothermal effect. However, the challenge of LM's uncontrollable shape-deformability hinders its stable application. To address this, we propose a straightforward and environmentally-friendly ice-bath ultrasonic treatment method to fabricate stable RSF-coated eutectic gallium indium (EGaIn) nanoparticles (RSF@EGaIn NPs). Additionally, a double-crosslinked hydrogel (RSF-P-EGaIn) is prepared by incorporating poly N-isopropyl acrylamide (PNIPAAm) and RSF@EGaIn NPs, leading to improved mechanical properties and temperature sensitivity. Our findings reveal that RSF@EGaIn NPs exhibit excellent stability, and the use of near-infrared (NIR) irradiation enhances the antibacterial behavior of RSF-P-EGaIn hydrogel in vivo. In fact, in vivo testing demonstrates that wounds treated with RSF-P-EGaIn hydrogel under NIR irradiation completely healed within 14 days post-trauma infection, with the formation of new skin and hair. Histological examination further indicates that RSF-P-EGaIn hydrogel promoted epithelialization and well-organized collagen deposition in the dermis. These promising results lay a solid foundation for the future development of drug release systems based on photothermal-responsive hydrogels utilizing RSF-P-EGaIn.

A Multifunctional Bimetallic Nanoplatform for Synergic Local Hyperthermia and Chemotherapy Targeting HER2-Positive Breast Cancer.

Anti-HER2 (human epidermal growth factor receptor 2) therapies significantly increase the overall survival of patients with HER2-positive breast cancer. Unfortunately, a large fraction of patients may develop primary or acquired resistance. Further, a multidrug combination used to prevent this in the clinic places a significant burden on patients. To address this issue, this work develops a nanotherapeutic platform that incorporates bimetallic gold-silver hollow nanoshells (AuAg HNSs) with exceptional near-infrared (NIR) absorption capability, the small-molecule tyrosine kinase inhibitor pyrotinib (PYR), and Herceptin (HCT). This platform realizes targeted delivery of multiple therapeutic effects, including chemo-and photothermal activities, oxidative stress, and immune response. In vitro assays reveal that the HCT-modified nanoparticles exhibit specific recognition ability and effective internalization by cells. The released PYR inhibit cell proliferation by downregulating HER2 and its associated pathways. NIR laser application induces a photothermal effect and tumor cell apoptosis, whereas an intracellular reactive oxygen species burst amplifies oxidative stress and triggers cancer cell ferroptosis. Importantly, this multimodal therapy also promotes the upregulation of genes related to TNF and NF-κB signaling pathways, enhancing immune activation and immunogenic cell death. In vivo studies confirm a significant reduction in tumor volume after treatment, substantiating the potential effectiveness of these nanocarriers.

Devastating the Supply Wagons: Multifaceted Liposomes Capable of Exhausting Tumor to Death via Triple Energy Depletion.

The anabolism of tumor cells can not only support their proliferation, but also endow them with a steady influx of exogenous nutrients. Therefore, consuming metabolic substrates or limiting access to energy supply can be an effective strategy to impede tumor growth. Herein, a novel treatment paradigm of starving-like therapy-triple energy-depleting therapy-is illustrated by glucose oxidase (GOx)/dc-IR825/sorafenib liposomes (termed GISLs), and such a triple energy-depleting therapy exhibits a more effective tumor-killing effect than conventional starvation therapy that only cuts off one of the energy supplies. Specifically, GOx can continuously consume glucose and generate toxic H2O2 in the tumor microenvironment (including tumor cells). After endocytosis, dc-IR825 (a near-infrared cyanine dye) can precisely target mitochondria and exert photodynamic and photothermal activities upon laser irradiation to destroy mitochondria. The anti-angiogenesis effect of sorafenib can further block energy and nutrition supply from blood. This work exemplifies a facile and safe method to exhaust the energy in a tumor from three aspects and starve the tumor to death and also highlights the importance of energy depletion in tumor treatment. It is hoped that this work will inspire the development of more advanced platforms that can combine multiple energy depletion therapies to realize more effective tumor treatment.

Synergistic photothermal catalytic oxidation of methanol and toluene mixture over Co-MOFs-derived catalyst: Interfacial and promotion effects

Herein, a series of Co/CoOx@CN nanocomposites with tunable synergistic interfacial structure were successfully obtained by a facile pyrolysis treatment. Among these nanocomposites, the nanocomposite pyrolyzed at 500 °C (denoted as Co/CoOx@CN-500) exhibited the best methanol photothermal catalytic activity (97% methanol conversion and 96% CO2 yield) under simulated solar irradiation. Compared with Co nanoparticles and Co@CN, Co/CoOx@CN-500 showed higher methanol photothermal catalytic activity. Moreover, the Co/CoOx@CN-500 also displayed a good ability to degrade methanol under outdoor natural solar. Such excellent photothermal activity mainly benefited from the strong interaction between the metals Co, CoOx and graphitic-carbon, which enhanced the photothermal effect by inducing the high work temperature, and the surface adsorb oxygen species of Co/CoOx@CN-500. Besides, the promotion effect of methanol on toluene oxidation was observed over Co/CoOx@CN-500 for the photothermal catalytic oxidation of the methanol and toluene mixture relative to their individual component. Water molecules did not affect the promotion effect of methanol on the photothermal catalytic oxidation of toluene. Reaction mechanism studies showed that 2-methylbenzaldehyde, the key intermediate formed by the reaction of formaldehyde generated by methanol oxidation and toluene, is the main reason for the improvement of the oxidation activity of toluene.

"Three-in-One" Multifunctional Hollow Nanocages with Colorimetric Photothermal Catalytic Activity for Enhancing Sensitivity in Biosensing.

Immunochromatographic assays (ICAs) have been widely used in the field detection of mycotoxin contaminants. Nevertheless, the lack of multisignal readout capability and the ability of signaling tags to maintain their biological activity while efficiently loading antibodies remain a great challenge in satisfying diverse testing demands. Herein, we proposed a novel three-in-one multifunctional hollow vanadium nanomicrosphere (high brightness-catalytic-photothermal properties)-mediated triple-readout ICA (VHMS-ICA) for sensitive detection of T-2. As the key to this biosensing strategy, vanadium was used as the catalytic-photothermal characterization center, and natural polyphenols were utilized as the bridging ligands for coupling with the antibody while self-assembling with formaldehyde cross-linking into a hollow nanocage-like structure, which offers the possibility of realizing a three-signal readout strategy and improving the coupling efficiency to the antibody while preserving its biological activity. The constructed sensors showed a detection limit (LOD) of 2 pg/mL for T-2, which was about 345-fold higher than that of conventional gold nanoparticle-based ICA (0.596 ng/mL). As anticipated, the detection range of VHMS-ICA was extended about 8-fold compared with the colorimetric signal alone. Ultimately, the proposed immunosensor performed well in maize and oat samples, with satisfactory recoveries. Owing to the synergistic and complementary interactions between distinct signaling modes, the establishment of multimodal immunosensors with multifunctional tags is an efficient strategy to satisfy diversified detection demands.