Background: Mithun (Bos frontalis) experience infestations from various ectoparasites, including several types of flies that cause painful bites, stress, blood loss and transmit a range of microorganisms. In the Mithun-inhabited forested regions of Phek, Nagaland, the Tabanus fly is particularly prevalent. Methods: Researchers studied Tabanus flies infesting free-range mithun in Phek District, Nagaland. The flies were collected and processed at the ICAR-NRC on Mithun in Nagaland. Both morphological (using stereoscopic microscopes) and molecular methods (employing ribosomal and mitochondrial gene markers) were utilized for identification. Additionally, DNA barcoding was done at Eurofins Genomics India Pvt. Ltd. to further ascertain the flies’ identities. Climate attributes (Temperature, Rainfall and Humidity) was recorded during the study. Result: Through morphological and molecular investigations, it was conclusively determined to be Tabanus rufofrater. The highest occurrence of Tabanus rufofrater encounters takes place between May and August, aligning with the pre-monsoon and monsoon seasons, before gradually declining thereafter. The prevalence of Tabanus rufofrater attacks peaks from May to August, coinciding with the pre-monsoon and monsoon periods and subsequently decreases.
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