Phase-Feeding Strategies Research Articles

252 Evaluation of phase feeding and complete diet blending at different standardized ileal digestible lysine levels on finishing pig growth performance, carcass characteristics and diet costs

Abstract A total of 2,160 mixed-gender pigs [PIC 337 × 1050; initial body weight (BW) = 24.9 ± 0.97 kg) were used in the 120-d study to compare feeding strategies (phase feeding vs complete diet blending) at different SID Lys levels (90 vs 100% of suggested requirement estimates) on finishing pig growth performance, carcass characteristics, and economics. Pens of 27 pigs were assigned to 1 of 4 treatments in a completely randomized block design with barn and initial BW as blocking factors resulting in 20 pens/treatment. The treatments included two feeding programs: a 5-phase feeding strategy at either 90% (Phase-90) or 100% of SID Lys requirement estimates (Phase-100) or blending of complete diets with pre-defined mixing proportions of a low and high SID Lys diet to meet 90 (Blend-90) or 100% (Blend-100) of the SID Lys curve requirement estimates for 23 to 127 kg pigs. Lysine levels were based on PIC 2021 requirement estimates. The 90% SID Lys diets were formulated to contain 4.38, 3.73, 3.06, 2.66 and 2.44 g SID Lys per Mcal NE for phases 1 to 5, respectively. The 100% SID Lys diets were formulated to contain 4.86, 4.15, 3.40, 2.96 and 2.71 g SID Lys per Mcal NE for phases 1 to 5, respectively. Pigs in phase-feeding strategies were fed on a feed budget with 21, 65, 67, 63 and 62 kg per pig for phases 1 to 5, respectively. Final BW and overall average daily gain (ADG; d 0 to 120) were not affected by the feeding strategy nor by diet SID Lys levels. In contrast, overall average daily feed intake (ADFI0 of pigs fed by diet blending was less than those fed by phase feeding (P = 0.002) resulting in increased gain to feed ratio (G:F; P < 0.001) for pigs fed by diet blending (table). The SID Lys levels did not influence overall ADFI or G:F. Hot carcass weight, carcass yield, percentage lean, fat depth and loin depth were not affected by the feeding program. Despite the decreased overall ADFI and increased G:F of pigs fed by diet blending, feeding strategy resulted in no significant differences in economic criteria except for reduced feed cost per kg gain at high price scenario (P = 0.049) for diet blending. With low diet cost, the 90% SID Lys level resulted in decreased feed cost per pig and feed cost per kg gain than 100% SID Lys (P < 0.001), but differences were not detected in income over feed cost (IOFC). In conclusion, diet blending at either 90 or 100% of the SID Lys requirement estimate reduced ADFI and increased G:F without impacting average daily gain (ADG) or carcass characteristics. At ingredient prices used in this study, feeding strategies at either 90 or 100% SID Lys did not significantly affect IOFC, but feeding 90% of SID Lys recommendation reduced feed cost.

51. Commercial evaluation of a phase feeding strategy compared to a single diet strategy for grower-finisher pigs

51. Commercial evaluation of a phase feeding strategy compared to a single diet strategy for grower-finisher pigs

93 Equivalent Effects of Growing Phase ADG and DOF at Various Placement Weights on Finishing Beef Cattle Performance Using Meta-analysis Approach

Abstract A meta-analysis approach was used to interpret effects of growing phase days and rate of gain on finishing performance at theoretically similar maturity using a dataset derived from 53 manuscripts. Growing phase feeding strategies included dry-lot feeding a high-energy finishing diet post-weaning (HG; n = 16), silage (DL-forg; n = 32) or a moderate energy diet (DL; n = 78), stocking cattle on winter wheat (WW; n = 16) or range (GR; n = 43). Independent variables of interest were initial growing phase body weight (initial BW), growing phase average daily gain (ADG), days-on-feed in growing phase (DOF), and final growing phase body weight. Data were analyzed using the PROC MIXED procedure of SAS, with treatment means weighted by experimental units (EU) per treatment. Optimal combinations of growing phase DOF and initial BW differed between models. Yet, for dressing percent (R2 = 0.39), LMA (R2 = 0.55), final finishing BW (R2 = 0.58), and HCW (R2 = 0.54) models, an 80-d growing phase for cattle starting at 240 kg resulted in greatest responses by these variables. Simulations evaluating effects of growing phase ADG on finishing performance (dressing percent; LMA; final body weight; HCW) were set equivalent to simulations evaluating the sensitivity of growing phase DOF in response to initial BW. Solving these simulations generated the initial BW at which cattle were more responsive to growing phase length (DOF) than ADG (LMAcritical point = 255 kg; DPcritical point = 242 kg; Final finishing BWcritical point = 231 kg; HCWcritical point = 233 kg). For finishing performance traits listed, each critical point represents the inflexion at which growing phase ADG exerts greatest influence on each trait modeled. Thus, for example, for cattle starting a growing phase at under 233 kg, faster growing phase ADG results in greater hot carcass weight response.

4 Protein and Amino Acid Concepts and Use in Swine Nutrition: Gary Allee’s Contributions to the Swine Industry

Abstract I was very fortunate to meet Gary Allee in 1984 when I began my M.S. degree at Kansas State University. I’ll remember Gary most in that he cared about people and truly wanted to serve humanity and make the world a better place through animal agriculture. He helped develop scholars, both nationally and internationally, that would be the future of our industry. Gary was proficient in seeking a solution to a problem and finding the experimental resources to explain it. Very early in his career, he helped verify the concept of a lysine:calorie ratio as a means of explaining the previously varied and inconsistent response to added fat in swine diets. Early research outlined the order of limiting amino acids in various feed ingredients. Gary’s research also focused on determining the nutritional value of protein sources for weanling pigs, such as dried whey, fish meal and dried skim milk that ultimately led to phase feeding strategies for early weaned pigs that are the backbone of our industry. Gary and his students determined lysine and other amino acid requirements for growing pigs and sows under field conditions. He helped elucidate the effects of low-protein, amino acid fortified diets under heat stress environments. As market weights increased, his research was instrumental in determining how to feed heavy weight pigs as well as those fed ractopamine. Gary grasped concepts and applied them into practical solutions in swine nutrition. He was a strong believer and leader in cooperative research among universities but also, at that time, a novel concept of university-industry partnerships. Those of us that can say they knew Gary Allee, are very fortunate and better because of it.

Increasing Dietary Lysine Impacts Differently Growth Performance of Growing Pigs Sorted by Body Weight.

Simple SummaryThe increasing demand on animal products expected for the next decades requires animal production systems to become more efficient in resource use. Most commercial operations feed all pigs the same feed at a determined time depending on the average BW of the batch, but without considering the variability of the population. However, low body weight (BW) pigs have been related to extra costs as reduced barn utilization, losses due to the poor carcass grading and inefficiency of phase feeding strategies. Some studies have previously hypothesized the need to do different phase feeding strategies to pigs sorted by initial BW. This work aimed to compare the effect of increasing the standardized ileal digestible lysine to net energy ratio (SID Lys:NE) over the performance of growing pigs sorted by initial BW in 3 categories (small, medium, and large). The results showed that small pigs could use more efficiently high SID Lys:NE diets compared to the large pigs during the growing phase (28–63 kg). The conclusions imply positive effects of feeding higher dietary lysine to small pigs to compensate for their reduced feed intake capacity. This strategy might improve growth rate and feed efficiency, without increasing feed costs per kg gain.An experiment was conducted analyzing whether growing pigs classified in different initial body weight categories (BWCAT) have a different response to increasing standardized ileal digestible lysine to net energy ratio (SID Lys:NE), to assess whether light pigs might benefit from being differentially fed. A total of 1170 pigs in pens of 13 were individually weighed, classified in 3 BWCAT (Lp: 32.1 ± 2.8 kg, Mp: 27.5 ± 2.3 kg, and Sp: 23.4 ± 2.9 kg), and afterwards pens were randomly allocated to 5 dietary SID Lys:NE treatments (3.25 to 4.88 g/Mcal) fed over 47 days. Results reported a greater linear improvement of growth and feed efficiency of Sp compared to Lp when increasing SID Lys:NE. Modelling the response to SID Lys:NE using quadratic polynomial models showed that the levels to reach 98% of maximum growth from day 0–47 were 3.67, 3.88, 4.06 g SID Lys/Mcal NE for Lp, Mp, and Sp, respectively. However, due to the overlapping SID Lys:NE confidence intervals at maximum performance, it was not possible to determine if requirements were different between BWCAT. Summarizing, the results suggested that feeding small pigs greater SID Lys:NE than large pigs can improve their performance and increase the efficiency of the overall production system.

Phase-feeding strategies based on lysine specifications for grow-finish pigs1.

Four experiments were conducted using 1,100 to 1,188 pigs each (PIC 359 × 1,050) from ~27 to 127 kg BW to evaluate phase-feeding strategies based on Lys specifications and number of dietary phases for grow-finish pigs. Different phase-feeding strategies were used in each experiment with treatments consisting of a combination of 3 Lys specifications at 96%, 98%, or 100% of estimated requirement for growth rate and 4 phase-feeding strategies with 1, 2, 3, or 4 dietary phases. A single-phase-feeding strategy reduced (P < 0.05) overall growth performance, live BW, and HCW whether Lys specifications were at 98% or 100% of estimated requirements compared with multi-phase-feeding strategies. Lysine specifications at 96% of estimated requirements in a 4-phase-feeding strategy reduced (P < 0.05) overall growth performance compared with feeding strategies with Lys at 100% of estimated requirements, unless Lys specifications were increased to 100% of estimated requirements in the late finishing phase. Lysine specifications at 98% or 100% of estimated requirements in a 2-, 3- or 4-phase feeding strategy led to similar (P < 0.05) overall growth rate, live BW, and HCW of grow-finish pigs. Pigs fed 1, 2, or 3-phase feeding strategies or feeding strategies with Lys below the requirements in early grow finish had improved growth performance driven by improved feed efficiency in the period following low Lys levels, indicating the occurrence of compensatory growth. For carcass characteristics, there was no evidence (P > 0.10) for differences in carcass yield, back fat, loin depth, or lean percentage across feeding strategies in any of the experiments. In conclusion, phase-feeding strategies provide performance advantages over feeding a single dietary phase throughout the grow-finish period. Simplification of feeding strategies from 4 to 3 or 2 dietary phases with Lys specifications at 98% to 100% of estimated requirements for growth rate does not compromise overall growth performance and carcass characteristics of grow-finish pigs from 27 to 127 kg BW. Although, using feeding programs with fewer dietary phases and Lys set slightly below the requirements can compromise growth performance if initial BW and feed intake in the grow-finish period are lower than expected.

Phase-Feeding the Beef Herd for Improved Feed Utilization

The dairy industry utilizes the concept of differences in nutrient requirements of different cows within the herd and manages nutrient supply accordingly. In that regard, the dairy industry approaches different cow nutrient requirements by implementing phase feeding in the cow herd. Phase feeding is defined as: changing the nutrient concentrations in a series of diets formulated to meet an animal's nutrient requirements more precisely at a particular stage of growth or production. Phase feeding in the dairy industry is implemented based on placing cows into multiple feeding groups based on their lactation status. The phase feeding strategy is utilized to address the different nutrient requirements associated with milk production intensity. The dairy industry addresses the nutrient requirements of cows not on a singular basis but on a multiple nutrient basis. This document is AN157, one of a series of the Animal Sciences Department, UF/IFAS Extension. Original publication date October 2005.&#x0D; AN157/AN157: Phase-Feeding the Beef Herd for Improved Feed Utilization (ufl.edu)

Effect of Phase-Feeding Strategies on Growth Performance and Carcass Characteristics of Growing-Finishing Pigs: Strategies to Reduce Dietary Phases Using a Field Approach on Lysine Levels

Effect of Phase-Feeding Strategies on Growth Performance and Carcass Characteristics of Growing-Finishing Pigs: Strategies to Reduce Dietary Phases Using a Field Approach on Lysine Levels

180 Jr. Platform Speaker: Evaluation of Phase-Feeding Strategies and Lysine Specifications for Grow-Finish Pigs.

180 Jr. Platform Speaker: Evaluation of Phase-Feeding Strategies and Lysine Specifications for Grow-Finish Pigs.

144 Evaluation of Phase-Feeding Strategies and Lysine Specifications for Grow-Finish Pigs on Growth Performance and Carcass Characteristics.

144 Evaluation of Phase-Feeding Strategies and Lysine Specifications for Grow-Finish Pigs on Growth Performance and Carcass Characteristics.

The Effect of Phase-Feeding Strategies on Growth Performance and Carcass Characteristics of Growing-Finishing Pigs: I. Lysine Levels at the Estimated Requirement

The Effect of Phase-Feeding Strategies on Growth Performance and Carcass Characteristics of Growing-Finishing Pigs: I. Lysine Levels at the Estimated Requirement

The Effect of Phase-Feeding Strategies on Growth Performance and Carcass Characteristics of Growing-Finishing Pigs: II. Field Approach on Lysine Levels

The Effect of Phase-Feeding Strategies on Growth Performance and Carcass Characteristics of Growing-Finishing Pigs: II. Field Approach on Lysine Levels

Physiological-phased kinetic characteristics of microalgae Chlorella vulgaris growth and lipid synthesis considering synergistic effects of light, carbon and nutrients

To comprehensively understand kinetic characteristics of microalgae growth and lipid synthesis in different phases, a phase-feeding strategy was proposed to simultaneously regulate light, carbon and nutrients in adaption, growth and stationary phases of microalgae cultivation. Physiological-phased kinetic characteristics of microalgae Chlorella vulgaris growth and lipid synthesis under synergistic effects of light, carbon and nutrients were investigated, and supply-demand relationships of electrons and energy between light and dark reactions of photosynthesis process were discussed. Finally, the optimized cultivation strategy for microalgae in various phases were obtained, under which the lipid productivity was significantly improved from 130.11 mg/L/d to 163.42 mg/L/d. The study provided some important guidance for the large-scale production of biofuels from microalgae.

Phase-feeding strategy for Chlorella vulgaris to enhance biomass and lipid productivity

A phase-feeding strategy of nutrients based on requirements of Chlorella vulgaris in different physiological phases was examined to maximize the biomass and lipid productivity. This strategy includes reduction of duration in adaption phase (stage-I), enhancement of biomass in growth phase (stage-II) and improvement of lipid productivity in stationary phase (stage-III). The duration of microalgae in adaption phase was reduced from 52 h to 34 h at nitrogen and phosphorus feeding rates of 5.11 mg/(L·d) and 0.54 mg/(L·d), whereas the maximum biomass concentration during growth phase was improved to (4.03±0.25) g/L at nitrogen and phosphorus feeding rates of 20.04 mg/(L·d) and 4.21 mg/(L·d). In stationary and decline phases, a maximum lipid productivity of 132.30 mg/(L·d) was achieved when nutrients supply was stopped at 128 h, which was 28.86 mg/(L·d) higher than that when nutrients supply was stopped at 104 h. This multi-phase cultivation could be a promising strategy for simultaneous enhancement of microalgae biomass and lipid productivity. Keywords: cultivation strategy, lipid productivity, microalgae, nutrients regulation DOI: 10.3965/j.ijabe.20171002.2735 Citation: Chang H X, Liao Q, Fu Q, Huang Y, Xia A, Zheng Y P, et al. Phase-feeding strategy for Chlorella vulgaris to enhance biomass and lipid productivity. Int J Agric & Biol Eng, 2017; 10(2): 205–215.

Evaluation of Phase Feeding Strategies and Lysine Specifications for Grow-Finish Pigs on Growth Performance and Carcass Characteristics

Evaluation of Phase Feeding Strategies and Lysine Specifications for Grow-Finish Pigs on Growth Performance and Carcass Characteristics

Evaluation of a phase-feeding strategy utilizing high-lipid high-fibre byproduct pellets in diets for feedlot steers

Two studies were conducted to evaluate the provision of high-lipid high-fibre byproduct pellets when used as a partial replacement (60% in Study 1 and 30% in Study 2; HLP) for barley grain and canola meal in finishing diets (BAR). The HLP was fed for the last 49, 98, or 147 d (HLP49, HLP98, and HLP147, respectively) in Study 1, and for the last 60 or 120 d in Study 2 (HLP60 and HLP120, respectively) or the last 60 d with additional canola oil (HLP60CO). The statistical model included the fixed effects of diet, period, and the interaction. Steers fed BAR147 had the greatest average daily gain (ADG) (P &lt; 0.01) and G:F (P = 0.01). The HLP147 had the greatest dry matter intake (DMI) during the first 49 d but least during the last 49 d of the finishing phase (treatment × period; P &lt; 0.01). Hot carcass weight for BAR147 and HLP49 were the heaviest (P = 0.04). In Study 2, DMI and ADG were not affected (P &gt; 0.05), but hot carcass weight was greater for BAR120 and HLP60 than HLP120 and HLP60CO. Inclusion of high-fibre high-lipid byproduct pellets in the latter part of the finishing period may improve carcass yield grade without affecting ADG and G:F.

Information Processing: Rate-Based Investigation of Cell Physiological Changes along Design Space Development

Recent initiatives summarized under the term quality by design (QbD) urge for science and risk-based pharmaceutical bioprocess development strategies. One of the most accepted concepts communicated by the regulatory authorities is the concept of design space-a multidimensional combination of critical process parameter (CPP) ranges where the quality acceptance criteria (critical quality attributes, CQAs) are fulfilled. Current design space development along QbD principles focuses on the investigation of statistical CPP/CQA interactions, while the biological mechanistic of this interaction is hardly considered. Furthermore, the plethora of available online and offline data gathered within design space development is typically not used for the demonstration of process understanding. Here we present a methodology about how typical recorded process data can be processed and used to gather mechanistic process knowledge within upstream design space development, without the need for further experiments or additional analytical procedures. Data derived from online and offline measurements (off gas quantification, air flows, substrate flows, biomass dry cell weight measurements) were processed into scale-independent information in the form of specific rates and yield coefficients (data processing). Subsequently, the obtained information was regressed with the investigated process parameters aiming at the investigation of mechanistic interactions (information processing). The power of the presented approach was demonstrated on a multivariate study involving two process parameters (induction phase temperature and induction phase feeding strategy) aiming at the production of recombinant product in an Escherichia coli K12 strain. The knowledge successfully extracted indicated a time dependency of the metabolic load posed on the system, a possible down regulation of the promoter at reduced temperatures, and reduced cell lysis at higher specific feeding regimes. The presented data and information processing methodology for mechanistic process knowledge extraction is fully complementary to the task of design space development for QbD submissions and can serve as the basis of mechanistic modeling. Manufacturing of pharmaceuticals intended for human use is under tight control of government authorities. To further improve product quality and allow more manufacturing flexibility, government agencies started to encourage manufactures to investigate and understand their manufacturing processes scientifically. This should lead to quality by design (QbD), hence a manufacturing that is so well understood that final product quality can be guaranteed by the manufacturing process itself.

The effects of polymer phase ratio and feeding strategy on solid–liquid TPPBs for the production of l-phenylacetylcarbinol from benzaldehyde using Candida utilis

To increase the bioproduction of L-phenylacetylcarbinol (PAC), a precursor molecule in the synthesis of the decongestants ephedrine and pseudoephedrine and which suffers from substrate, product, and by-product inhibition, by ensuring that the delivery of the substrate, benzaldehyde, is maintained within a strict concentration window. Beads of the commercial polymer, Hytrel G3548L, can act as a sequestering phase to reduce inhibitory effects to cells of Candida utilis while creating a reservoir for high concentrations of products. In this work we varied the polymer phase volume ratio (from 3 to 15%), and modified the benzaldehyde feeding strategy to further improve on system performance, resulting in greater than 100% increase in the PAC productivity relative to a single phase control, as well as robust operation of the two-phase bioreactor with minimal operator intervention.

Effect of Growth Phase Feeding Strategies on Succinate Production by Metabolically Engineered Escherichia coli

Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt showed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.

Comparison of Dairy Beef Genetics and Dietary Roughage Levels

The objectives of these trials were to investigate the performance of Jersey steers in relation to Holsteins under current management practices when fed diets differing in energy density and subsequent effects on carcass characteristics. In experiment 1, twelve Jersey and 12 Holstein steers were offered dietary treatments with differing roughage levels. Roughage levels investigated on a dry matter basis were 55% reduced to 25% versus 25% followed by 12.5% (HIGH and LOW, respectively) with all animals receiving the same finishing diet containing 6.5% roughage. Holstein steers were heavier than Jerseys at the initiation of the trial (228 vs. 116kg). A diet response was observed for gain efficiency during the first period in which LOW was greater than HIGH. Holstein steers had higher dry matter intakes and rates of gain than Jerseys. However, gain efficiency was better for Jersey steers during the first and last periods. Carcass traits were influenced by breed but not diet. Holsteins had heavier hot carcass weights, greater dressing percentages, more backfat, and larger longissimus muscle area, whereas marbling scores were similar to Jerseys. The increased efficiency of Jersey steers and significant reduction in carcass value due to light carcass weights suggested that Jersey steers should be fed to heavier live weights. Experiment 2 utilized 85 steers to investigate continuous feeding of a low-roughage, high-concentrate diet versus a phase-feeding strategy. Jersey (n=40) and Holstein (n=45) steers were assigned to a diet containing 20% corn silage on a dry matter basis (HEN) or a phase-feeding program (PHASE) in which corn silage was reduced from 60 to 40% followed by the same diet as HEN. Initial body weights were similar for dietary treatments but differed by breed. A diet response was observed for live weight at the end of the first and second period, first period average daily gain (ADG), overall ADG, and days on feed with HEN having higher ADG than PHASE and fewer days on feed. Breed affected all body weight and gain variables with Holsteins being heavier and gaining more rapidly than Jersey steers. Jersey carcasses were lighter, had the highest percentage trim loss, least amount of backfat, and lowest numerical yield grade. Holstein steers had a greater propensity for gain, whereas the Jersey steers were equally or more efficient. These findings suggest that phase feeding Jersey steers higher-roughage diets has minimal effect on carcass traits.