Peroxide Nanoparticles Research Articles

Hyaluronic acid coated mesoporous carbon-copper peroxide for H2O2 self-supplying and near-infrared responsive multi-mode breast cancer oncotherapy

It is challenging to develop the synergistic intelligent therapeutic nanoplatform to cure cancer. In the present study, a novel nanotherapeutic platform was constructed for H2O2 self-supplying and multimodal breast cancer therapy. In which, copper peroxide nanoparticles (CP NPs) were adsorbed on the surface of mesoporous carbon nanospheres (MCN) through electrostatic attraction, followed by loading doxorubicin (DOX) into the nanocomposite (MCN-CP) and coating hyaluronic acid (HA) on the surface, the DOX/MCN-CP-HA nanoplatform was obtained. In the system, the MCN not only possessed a high DOX loading capacity, but produced excellent photothermal therapy (PTT) effect. Importantly, the ultra-small CP NPs as the Fenton agent not only could selectively self-supplying H2O2 in acidic condition, but simultaneously release Cu2+ to catalyze the production of ·OH in the presence of H2O2. Meantime, the resulting Cu2+ possessed GSH-elimination property, which afforded enhanced chemodynamic therapy (CDT). Furthermore, the outer layer HA targeted to CD44 and achieved breast cancer cell targeting. The elevated temperature from PTT and acidic tumor microenvironment accelerated the release of DOX, which enabled DOX/MCN-CP-HA as an intelligent CDT-PTT-chemotherapy synergistic nanoplatform. In vitro and in vivo pharmacodynamic evaluations confirmed the potential of the nanoplatform for CDT-PTT-chemotherapy synergistic oncotherapy of breast cancer.

Antifungal Activity Changes of Zinc Oxide and Zinc Peroxide Nanoparticles

Antifungal Activity Changes of Zinc Oxide and Zinc Peroxide Nanoparticles

Polymer-coated calcium peroxide nanoparticles as an oxygen self-supplying platform for enhanced photodynamic therapy

Photodynamic therapy, employing photosensitizer catalysis to convert oxygen into cytotoxic reactive species upon light irradiation, holds high promise in tumor therapy due to its high selectivity and non-invasiveness. However, the anticancer efficacy is greatly limited by the hypoxia microenvironment of tumor tissue. Herein, we report an oxygen self-supplying nano-platform for photodynamic therapy, which is fabricated by calcium peroxide nanoparticles with surface modification of a copolymer, PMPS-b-P(NIPAAm-co-VBRB) by silanization. In this system, calcium peroxide nanoparticle acts as an oxygen donor while PMPS-b-P(NIPAAm-co-VBRB) plays the role of photosensitizer and can stabilize shell of calcium peroxide nanoparticles. The resultant polymer-coated nanoparticles have a uniform size of 228 nm measured by dynamic light scattering. The polymer-coated calcium peroxide nanoparticles show greater production efficiency of singlet oxygen to deplete 1,3-diphenylisobenzofuran than PMPS-b-P(NIPAAm-co-VBRB) upon the exposure to the LED@520 nm. The result demonstrates that the polymer-coated calcium peroxide nanoparticles are effective for oxygen generation and hold potential to enhance the efficacy of photodynamic therapy.

Immune-regulating strategy against rheumatoid arthritis by inducing tolerogenic dendritic cells with modified zinc peroxide nanoparticles

In hypoxic dendritic cells (DCs), a low level of Zn2+ can induce the activation of immunogenic DCs (igDCs), thereby triggering an active T-cell response to propel the immune progression of rheumatoid arthritis (RA). This finding indicates the crucial roles of zinc and oxygen homeostasis in DCs during the pathogenesis of RA. However, very few studies have focused on the modulation of zinc and oxygen homeostasis in DCs during RA treatment. Proposed herein is a DC-targeting immune-regulating strategy to induce igDCs into tolerogenic DCs (tDCs) and inhibit subsequent T-cell activation, referred to as ZnO2/Catalase@liposome-Mannose nanoparticles (ZnCM NPs). ZnCM NPs displayed targeted intracellular delivery of Zn2+ and O2 towards igDCs in a pH-responsive manner. After inactivating OTUB1 deubiquitination, the ZnCM NPs promoted CCL5 degradation via NF-κB signalling, thereby inducing the igDC-tDC transition to further inhibit CD4+ T-cell homeostasis. In collagen-induced arthritis (CIA) mice, this nanoimmunoplatform showed significant accumulation in the spleen, where immature DCs (imDCs) differentiated into igDCs. Splenic tDCs were induced to alleviate ankle swelling, improve walking posture and safely inhibit ankle/spleen inflammation. Our work pioneers the combination of DC-targeting nanoplatforms with RA treatments and highlights the significance of zinc and oxygen homeostasis for the immunoregulation of RA by inducing tDCs with modified ZnO2 NPs, which provides novel insight into ion homeostasis regulation for the treatment of immune diseases with a larger variety of distinct metal or nonmetal ions.Graphical

A study of the comparative effect of cerium oxide and cerium peroxide on stimulation of angiogenesis: Design and synthesis of pro-angiogenic chitosan/collagen hydrogels.

Poor angiogenesis at injury site is a major problem in chronic wounds, which could lead to limbs amputation in adverse cases. To overcome this issue, several efforts have made in literature and by our group as well to develop pro-angiogenic agents. For this purpose, metal oxides due to their oxidative potential have been studied and found very attractive agents. Cerium oxides are proven to be non-toxic and their biological studies have already proved their importance in preventing chronic inflammation, and neurological diseases among several others by modulating the intracellular reactive oxygen species. In current study, we report the synthesis and neovascularization activity of cerium oxide and cerium peroxide nanoparticles when loaded into chitosan and collagen hydrogel. The hydrogels were characterized by FTIR, SEM and XRD. The pro-angiogenic behavior of these hydrogels was studied by in-vivo CAM assay. It was found that cerium peroxide loaded material showed significantly increase in angiogenesis as compared to cerium oxide loaded materials. It was demonstrated that cerium peroxide hydrogels enhanced the angiogenic capability in CAM assay as compared to cerium oxide and hence holds good potential for chronic ulcer and burn wounds healing.

Chemical Factory-Guaranteed Enhanced Chemodynamic Therapy for Orthotopic Liver Cancer.

In the field of nanomedicine, there is a tendency of matching designed nanomaterials with a suitable type of orthotopic cancer model, not just a casual subcutaneous one. Under this condition, knowing the specific features of the chosen cancer model is the priority, then introducing a proper therapy strategy using designed nanomaterials. Here, the Fenton chemistry is combined with zinc peroxide nanoparticles in the treatment of orthotopic liver cancer which has a “chemical factory” including that liver is the main place for iron storage, metabolism, and also the main metabolic sites for the majority of ingested substances, guaranteeing customized and enhanced chemodynamic therapy and normal liver cells protection as well. The good results in vitro and in vivo can set an inspiring example for exploring and utilizing suitable nanomaterials in corresponding cancer models, ensuring well‐fitness of nanomaterials for disease and satisfactory therapeutic effect.

Mechanistic revelation into the degradation of organic pollutants by calcium peroxide nanoparticles@polydopamine in Fe(III)-based catalytic systems

Fenton (or Fenton-like) reaction is widely used to degrade organic pollutants in water. However, instability and insecurity of liquid H2O2, narrow pH range and the low circulation rate between Fe2+/Fe3+ have become the most critical limiting factors of this technology. To break this technical barrier, calcium peroxide nanoparticles @polydopamine composites (nCaO2@PDA) with core–shell structures were prepared, which showed desirable hydrogen peroxide (H2O2) release performance as well as excellent activity for RhB degradation in a wide pH range of 2.0 ∼ 11.0 in the presence of Fe3+. By careful analysis the surface properties of PDA, the Fe2+ concentrations during reaction and the results of rational-designed control experiments, it is proved that PDA can reduce Fe3+ through the phenol-quinone transformation. More importantly, thanks to the unique core–shell structure, abundance of H2O2 and Fe2+ are both available in the vicinity of nCaO2@PDA, ensuring the superior catalytic performance than that of nCaO2. In addition, the HO• in solution rather than those adsorbed on PDA surface contribute most in the RhB degradation.

Bacterial biofilm microenvironment responsive copper-doped zinc peroxide nanocomposites for enhancing chemodynamic therapy

Dental plaque, a kind of natural biofilm, has attracted much attention because of its role in caries and periodontal disease. The pathogenicity of the dominant bacterial species of S. mutans is based on the formation of extracellular polysaccharides (EPS) and its survival in acidic environments. In addition, the formed plaque biofilm has the characteristics of water insolubility, retention, and low membrane permeability. This is why there are no effective antibiotics orother ways to treat dental caries. Chemodynamic therapy (CDT) is an ideal therapeutic modality with biofilm microenvironment as stimulus. In this paper, a unique nanocomposite, ZnO2-Cu@RB NPs, has been prepared which is composed of copper-doped zinc peroxide nanoparticles (ZnO2-Cu NPs) and antibacterial agent Rose Bengal (RB). It can quickly (<10 min) and efficiently kill S. mutans (greater than 8.0 log10 CFU mL−1) in acidic biofilm via the in situ enhanced CDT, and significantly reduce the demineralization of apatite. ZnO2-Cu@RB NPs can also weaken the acid production of S. mutans and inhibit the formation of EPS. More significantly, excellent therapeutic efficacy against S. mutans in the rat model was observed. Our findings reveal the great potential of metal peroxide nanoparticles as a new strategy for combating biofilm-related diseases.

Impact of oxygen-calcium-generating and bone morphogenetic protein-2 nanoparticles on survival and differentiation of bone marrow-derived mesenchymal stem cells in the 3D bio-printed scaffold

Although stem cell therapy is a major area of interest in tissue engineering, providing proper oxygen tension, good viability, and cell differentiation remain challenges in tissue-engineered scaffolds. In this study, an osteogenic scaffold was fabricated using the 3D bio-printing technique. The bio-ink contained alginate hydrogel, encapsulated human bone marrow-derived mesenchymal stem cells (hBM-MSCs), calcium peroxide nanoparticles (CPO NPs) as an oxygen generating biomaterial, and bone morphogenic protein-2 nanoparticles (BMP2 NPs) as an osteoinductive growth factor. CPO NPs were synthesized with the hydrolysis–precipitation method, and their concentrations in the bio-ink were optimized. Scaffolds containing CPO 3% (w/w) were preferred, because they generated sufficient oxygen gas for 20 days, increased mechanical strength after 20 days, and had sufficient stability. The CPO NPs effect on the viability of embedded hBM-MSCs under hypoxic conditions was analyzed. Live/Dead staining results represented a 22% improvement in CPO 3% scaffold viability on day 7. Therefore, CPO NPs constituted a promising survival factor. BMP2 NPs were prepared with the double emulsification technique. The incorporation of both BMP2 and CPO NPs resulted in the upregulation of Runt-related transcription factor 2, Collagen type I alpha 1, and the osteocalcin genes compared to internal references in osteogenic media. Overall, the proposed 3D bio-printed osteogenic scaffold in this study has moved scientific research one step forward toward successful stem cell therapy and helped improve host tissue healing by biological activity enhancement, especially for low oxygen pressure tissues.

Reactive oxygen species-inducing titanium peroxide nanoparticles as promising radiosensitizers for eliminating pancreatic cancer stem cells

BackgroundDespite recent advances in radiotherapy, radioresistance in patients with pancreatic cancer remains a crucial dilemma for clinical treatment. Cancer stem cells (CSCs) represent a major factor in radioresistance. Developing a potent radiosensitizer may be a novel candidate for the eradication of pancreatic CSCs.MethodsCSCs were isolated from MIA PaCa-2 and PANC1 human pancreatic cancer cell lines. Titanium peroxide nanoparticles (TiOxNPs) were synthesized from titanium dioxide nanoparticles (TiO2NPs) and utilized as radiosensitizers when added one hour prior to radiation exposure. The antitumor activity of this novel therapeutic strategy was evaluated against well-established pancreatic CSCs model both in vitro and in vivo.ResultsIt is shown that TiOxNPs combined with ionizing radiation exhibit anti-cancer effects on radioresistant CSCs both in vitro and in vivo. TiOxNPs exhibited a synergistic effect with radiation on pancreatic CSC-enriched spheres by downregulating self-renewal regulatory factors and CSC surface markers. Moreover, combined treatment suppressed epithelial-mesenchymal transition, migration, and invasion properties in primary and aggressive pancreatic cancer cells by reducing the expression of proteins relevant to these processes. Notably, radiosensitizing TiOxNPs suppressed the growth of pancreatic xenografts following primary or dissociating sphere MIA PaCa-2 cell implantation. It is inferred that synergy is formed by generating intolerable levels of reactive oxygen species (ROS) and inactivating the AKT signaling pathway.ConclusionsOur data suggested the use of TiOxNPs in combination with radiation may be considered an attractive therapeutic strategy to eliminate pancreatic CSCs.

Embedding of zinc peroxide (ZnO2) nano-particles into PEDOT:PSS for fabricating a new PEDOT:PSS/ZnO2 system with promising thermoelectric functions

Recently, conducting polymers have been widely used in thermoelectric (TE) technology, owing to their bendability, easy processability, their low intrinsic thermal conductivity and their distinct TE functions. Thus, in this respect, a new TE system based on Poly(3,4-ethylene-dioxythiophene):Poly(styrenesulphonate) and Zinc peroxide nano-particles (PEDOT:PSS/ZnO2) has been constructed for TE purposes. The impact of ZnO2 content on the anisotropic (in plane//and through plane ⊥) TE functions has been investigated at 300 K. At ZnO2 = 60 wt%, PEDOT:PSS/ZnO2 system owns an optimal σ//∼1348.33 ± 27.5 S/m and σ⊥∼791.68 ± 30.9 S/m higher than σ//∼870.16 ± 26.5 S/m and σ⊥∼512.59 ± 12.58 S/m of PEDOT:PSS thanks to the great surface area, high dispersion, high electronegativity nature of ZnO2, besides, the strong interactions between ZnO2 and PEDOT:PSS, and the increase in the carrier mobility. The PEDOT:PSS/ZnO2 system owns an optimal S// ∼31.63 ± 0.35 μV/K and S⊥∼25.76 ± 0.20 μV/K at ZnO2 = 60 wt% higher than S// ∼15.5 ± 0.5 μV/K and S⊥∼10.66 ± 0.37 μV/K for PEDOT:PSS thanks to the decrease in the carrier concentration. The PEDOT:PSS/ZnO2 system owns an optimal PF//∼1.34 ± 0.04 μW/mK2 and PF⊥∼0.52 ± 0.04 μW/mK2 at ZnO2 = 60 wt% higher than PF//∼0.20 ± 0.04 μW/mK2 and PF⊥∼0.05 ± 0.01 μW/mK2 for PEDOT:PSS owing to the energy filtering effects at the interfaces and the increase in the carrier mobilities. Accordingly, the obtained zT// ∼4.26 ± 0.2 × 10─4 and zT⊥ ∼2.72 ± 0.1 × 10─4 have been achieved at 300 K for ZnO2 = 60 wt%. The system displays a high environmental (humid and air) stability. Finally, the suggested system which is based on PEDOT:PSS and peroxide metals shows promising TE functions for many applications such as electronic, temperature sensing and wearable technology.

Synthesis, characterization and application of calcium peroxide nanoparticles as a novel adsorbent for removal of malic acid from aqueous solutions

This study aims to propose a novel adsorbent for removal of malic acid from aqueous solutions. In this context, calcium peroxide (CaO2) nanoparticles as novel adsorbent were synthesized, characterized, and assessed the efficiency of in malic acid removal. Followed this, kinetic, thermodynamic, and equilibrium studies were conducted. The average particle diameter and surface area of CaO2 nanoparticles have been found to be 34.77 nm and 13.58 m2.g−1, respectively. The maximum adsorption capacity of CaO2 nanoparticles for malic acid has been determined as 596.86 mg.g−1. The results showed that CaO2 nanoparticles are effective adsorbent in removing malic acid from aqueous solutions.

Boosting Tumor Immunotherapy by Bioactive Nanoparticles via Ca2+ Interference Mediated TME Reprogramming and Specific PD‐L1 Depletion

AbstractThe clinical outcomes of programmed cell death protein‐1 (PD‐1)/programmed death ligand‐1 (PD‐L1) blockade are usually limited by the multiple immune evasion mechanism of the tumor, as well as the immune storm caused by “off‐target” effects of the PD‐1/PD‐L1 antibody. Here, a previously unknown strategy is proposed to synergize the “Ca2+ interference” mediated M2‐like tumor‐associated macrophages (TAM) re‐education and Ca2+‐activating locally PD‐L1 depletion for immunotherapy. The authors discover that calcium‐containing nanoparticles can induce significant “Ca2+ interference” effect and simultaneously reset TAM toward the M1 phenotype through activation of multiple inflammation‐related signaling pathways, as well as NLRP3 inflammasomes, and promoting in situ tumor‐associated antigen release. In addition, a circular aptamer‐DNAzyme conjugate (cAD) is designed with a tumor‐targeting ability and its catalytic shear activity can be specifically activated by Ca2+, which reduces potential autoimmune‐like disorders of PD‐L1 antibody. By simply integrating the ultra‐high pH‐sensitive calcium peroxide nanoparticles with cAD into one nanosystem, it is demonstrated that the nanosystem not only arrest primary tumor progression and lung metastasis but also provides a long‐term immunological memory, which can protect against tumor rechallenge. Therefore, this work provides an attractive strategy for boosting tumor immunotherapy.

Enhanced photodynamic therapy/photothermo therapy for nasopharyngeal carcinoma via a tumour microenvironment-responsive self-oxygenated drug delivery system

The hypoxic nature of tumours limits the efficiency of oxygen-dependent photodynamic therapy (PDT). Hence, in this study, indocyanine green (ICG)-loaded lipid-coated zinc peroxide (ZnO2) nanoparticles (ZnO2@Lip-ICG) was constructed to realize tumour microenvironment (TME)-responsive self-oxygen supply. Near infrared light irradiation (808 nm), the lipid outer layer of ICG acquires sufficient energy to produce heat, thereby elevating the localised temperature, which results in accelerated ZnO2 release and apoptosis of tumour cells. The ZnO2 rapidly generates O2 in the TME (pH 6.5), which alleviates tumour hypoxia and then enhances the PDT effect of ICG. These results demonstrate that ZnO2@Lip-ICG NPs display good oxygen self-supported properties and outstanding PDT/PTT characteristics, and thus, achieve good tumour proliferation suppression.

Acid-Triggered H2O2 Self-Supplying Nanoplatform for 19F-MRI with Enhanced Chemo-Chemodynamic Therapy.

The real-time tracking and efficacy evaluation of therapeutic nanoplatforms especially in deep-tissues is of great importance but faces challenges. Meanwhile, chemodynamic therapy (CDT), relying on Fenton reaction by converting H2O2 into toxic hydroxyl radicals (•OH), has drawn wide interests in the fabrication of nanozymes for tumor therapy, while endogenous H2O2 is usually insufficient for effective CDT. Here, we report the pH-responsive multifunctional nanoplatforms consisting of copper peroxide (CP) nanoparticles, paclitaxel (PTX) and perfluoro-15-crown-5-ether (PFCE), for 19F magnetic resonance imaging guided and enhanced chemo-chemodynamic synergetic therapy with self-supplied H2O2 stemmed from the decomposition of CP nanoparticles under acid conditions in tumor. The decomposition of CP nanoparticles further promotes the release of PTX for enhanced chemotherapy. Both in vitro and in vivo results indicate that the efficient generation of •OH and drug release effectively inhibits tumor growth. Furthermore, 19F MRI signal can clearly track the fate of nanoplatforms in tumor and guide tumor treatment. This work provides a promising strategy for the rational design and construction of multifunctional nanoplatforms for imaging-guided synergistic therapy of deep seated tumor.

Simultaneous application of CaO2 nanoparticles and microbial consortium in Small Bioreactor Chambers (SBCs) for phenol removal from groundwater

Phenols are toxic products derived from a wide range of industrial activities and the entry of these contaminants into the environment is seriously hazardous for both human and aquatic ecosystems. This study presents the use of a phenol-degrading consortium surrounded in an innovative “Small Bioreactor Chambers” (SBCs) along with biostimulation for phenol bioremediation from contaminated groundwater. To investigate the effect of biostimulation, synthesized-calcium peroxide (CaO2) nanoparticles were applied. The phenol-degrading consortium, was successfully isolated from a water well located in Iran. The highest growth rate and phenol degradation of the ph100-consortium were observed at 15 °C and pH 7.5 in the presence of 500 mg·L-1 of powdered CaO2 and 100 mg·L-1 of phenol as a sole source of carbon. Next-generation sequencing (NGS) analysis of the consortium revealed that Proteobacteria and Bacteroidetes with 75.3% and 16.2% relative abundance were the dominant phyla in the ph100-consortium, respectively. Experimental results indicated that 100% of the contaminant (100 mg·L-1) was successfully removed from groundwater using encapsulated CaO2 within 60 days with the negligible negative impacts on the microbial population. Furthermore, the highest biodegradation percentage were achieved during incubation of SBCs in a medium supplemented with 500 mg·L-1 CaO2 powder in 25th days of experiment. These results provide certain evidence for a successful simultaneous application of biostimulation and bioaugmentation processes through SBCs for contaminated water treatment.

Enhanced Chemodynamic Therapy by Cu-Fe Peroxide Nanoparticles: Tumor Microenvironment-Mediated Synergistic Fenton Reaction.

An urgent need in chemodynamic therapy (CDT) is to achieve high Fenton catalytic efficiency at small doses of CDT agents. However, simple general promotion of the Fenton reaction increases the risk of damaging normal cells along with the cancer cells. Therefore, a tailored strategy to selectively enhance the Fenton reactivity in tumors, for example, by taking advantage of the characteristics of the tumor microenvironment (TME), is in high demand. Herein, a heterogeneous CDT system based on copper-iron peroxide nanoparticles (CFp NPs) is designed for TME-mediated synergistic therapy. CFp NPs degrade under the mildly acidic conditions of TME, self-supply H2O2, and the released Cu and Fe ions, with their larger portions at lower oxidation states, cooperatively facilitate hydroxyl radical production through a highly efficient catalytic loop to achieve an excellent tumor therapeutic efficacy. This is distinct from previous heterogeneous CDT systems in that the synergism is closely coupled with the Cu+-assisted conversion of Fe3+ to Fe2+ rather than their independent actions. As a result, almost complete ablation of tumors at a minimal treatment dose is demonstrated without the aid of any other therapeutic modality. Furthermore, CFp NPs generate O2 during the catalysis and exhibit a TME-responsive T1 magnetic resonance imaging contrast enhancement, which are useful for alleviating hypoxia and in vivo monitoring of tumors, respectively.

A DNA functionalized metal–organic framework combined with magnesium peroxide nanoparticles: targeted and enhanced photodynamic therapy

A self-supplying O2 nanosystem (Hf-MOF-MgO2/DNA) based on a DNA functionalized MOF combined with magnesium peroxide nanoparticles was constructed. This nanosystem can be used for targeted PDT with a significantly enhanced therapeutic efficacy.

Targeting Tumor Microenvironment by Metal Peroxide Nanoparticles in Cancer Therapy.

Solid tumors have a unique tumor microenvironment (TME), which includes hypoxia, low acidity, and high hydrogen peroxide and glutathione (GSH) levels, among others. These unique factors, which offer favourable microenvironments and nourishment for tumor development and spread, also serve as a gateway for specific and successful cancer therapies. A good example is metal peroxide structures which have been synthesized and utilized to enhance oxygen supply and they have shown great promise in the alleviation of hypoxia. In a hypoxic environment, certain oxygen-dependent treatments such as photodynamic therapy and radiotherapy fail to respond and therefore modulating the hypoxic tumor microenvironment has been found to enhance the antitumor impact of certain drugs. Under acidic environments, the hydrogen peroxide produced by the reaction of metal peroxides with water not only induces oxidative stress but also produces additional oxygen. This is achieved since hydrogen peroxide acts as a reactive substrate for molecules such as catalyse enzymes, alleviating tumor hypoxia observed in the tumor microenvironment. Metal ions released in the process can also offer distinct bioactivity in their own right. Metal peroxides used in anticancer therapy are a rapidly evolving field, and there is good evidence that they are a good option for regulating the tumor microenvironment in cancer therapy. In this regard, the synthesis and mechanisms behind the successful application of metal peroxides to specifically target the tumor microenvironment are highlighted in this review. Various characteristics of TME such as angiogenesis, inflammation, hypoxia, acidity levels, and metal ion homeostasis are addressed in this regard, together with certain forms of synergistic combination treatments.

Interaction of zinc peroxide nanoparticle with fibroblast cell

This work demonstrates the structural interaction of the as-synthesized zinc peroxide (ZnO2) nanoparticles with fibroblast cells (FBC). The ZnO2 nanoparticles (ZNP) of desired sizes (10-20 nm) are synthesized, and the purity and structural confirmations are studied using various imaging and spectroscopic techniques. FBC (buffalo) lines are cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and penicillin (100 µg/mL), and with non-essential amino acid and vitamin as additional ingredients, followed by incubation at 37°C with continuous purging of the chamber using 5% CO2. The fluorescent microscopic images are captured for the initial healthy and cultured FBCs, and after pouring the nanoparticles in the cultured FBCs. Healthy cell-growth is noticed during the cell culture process suggesting the formation of ZNP-FBC complexes without contamination and coagulation. After allowing the interaction of ZNPs with the FBCs, the presence of ZNPs only on the cell sites are observed without coagulation of ZNPs in the cell areas, suggesting the selective interference of ZNPs on the surface of the grown cell. The understanding of the interaction process of the ZNPs with the living cell, would provide the practical utilization of the ZNPs in nanomedicine and nano-drug delivery. Copyright © 2017 VBRI Press.