Objectives: To determine the prevalence of proton pump inhibitor use among the junior doctors in a tertiary health care centre of Manipur & to assess the practice of proton pump inhibitor use among them.Materials & Methodology: A cross-sectional study was conducted among the junior doctors i.e. internees, house officers and post graduate students in a tertiary health care centre of Manipur during November to January 2012. Respondents were purposively selected and data were collected using structured interview schedule which consists of socio-demographic variables, questions on use of PPI. Those who refused to participate and who could not be contacted even after 3 successive visits were excluded from the study. Data were analysed using SPSS 20 & was presented in mean, percentages etc.Results: Prevalence of PPI use was 66.6%. All the doctors used PPI only on selective patients. Majority of them use for gastrointestinal diseases (84%). Little more than 9 in 10 participants used PPI for the patients aged 20-30 years. Majority of them used PPI for 2-4 wks in Acid Peptic Disorder (30.5%) and less than 1week in Non Acid Peptic Disorder. More than 8 in 10 participants told that PPI was the best drug for acid peptic disorder and majority opined that PPI should not be used randomly for all gastro-intestinal disorder.Conclusion: Inappropriate use of PPI remains rampant in hospital practice. Increased awareness should be created among the clinicians so that appropriate prescription of PPIs could improve the patient care.