Extraction Of Pectin Research Articles

Microwave and ultrasound-assisted natural deep eutectic solvents-based extraction of pectin from onion peel wastes

ABSTRACT Pectin was extracted from onion peels using microwave and ultrasound-assisted natural deep eutectic solvents (NADES) and compared with conventional acid-extracted pectin. The primary screening trials indicated that the suitable combination of hydrogen bond acceptor and donor was choline chloride and tartaric acid, respectively. The Box-Behnken design was further used to optimize processing conditions like microwave intensity (26.903 W/g), microwave processing duration (2.99 min), and sonication duration (10.18 min) for maximizing the pectin yield from onion peel powder. The moisture, ash, and equivalent weight of extracted pectin were in the range specified by the International Pectin Producers Association. The higher anhydrouronic acid content of the NADES-extracted pectin indicated the higher purity of pectin compared to the conventional acid-extracted pectin. The NADES-based extraction was found to be a promising system for extracting pectin with a higher degree of esterification from onion peels.

High Pectin Recovery from Cocoa Husks Using an Autoclave Approach: An Analysis of Its Physicochemical, Structural, and Genotoxicity Properties.

Pectin was extracted from cocoa husks, a food-processing biowaste, using an autoclave approach. A Box-Behnken design (BBD) and response surface methodology (RSM) were used to optimize pectin extraction. Three factors including extraction time (5-40 min), temperature (105-135 °C), and solid to liquid ratios (SLRs) (10-30 w/v) were employed. Results showed that the optimal conditions for high cocoa-husk-pectin (CHP) yield of 26.22% was 105 °C for 5 min with an SLR at 20 w/v. The physicochemical characteristics of CHP were compared with commercial high-methoxyl pectin (CHMP) and commercial low-methoxyl pectin (CLMP). CHP was classified as low-methoxyl pectin, with a degree of esterification at 34.74% and methoxyl content of 5.08%. The galacturonic acid content of CHP was 32.71% which was lower than CHMP (72.69%) and CLMP (41.24%). The intrinsic viscosity and viscosity-average molecular weight was similar to CLMP but higher than CHMP. No significant differences in water-holding capacity were found among samples. CHP showed higher oil-holding capacity but lower solubility compared with commercial pectin. CHP solutions showed pseudoplastic behavior. The viscosities of CHP solutions improved at increasing concentrations and decreasing pH. The CHP solution viscosities were lower than CLMP at the same condition. The viscoelastic properties of CHP solutions increased at higher concentrations, with the optimal value at pH 3. CHP showed no genotoxicity when assayed using the Ames test. Autoclave extraction as an accessible fast method showed potential for high pectin yield recovery from cocoa husks.

Therapeutic Potential of Pectin and Its Derivatives in Chronic Diseases.

Non-communicable diseases (NCDs) are described as a collection of chronic diseases that do not typically develop from an acute infection, have long-term health effects, and frequently require ongoing care and therapy. These diseases include heart disease, stroke, cancer, chronic lung disease, neurological diseases, osteoporosis, mental health disorders, etc. Known synthetic drugs for the treatment or prevention of NCDs become increasingly dangerous over time and pose high risks due to side effects such as hallucination, heart attack, liver failure, etc. As a result, scientists have had to look for other alternatives that are natural products and that are known to be less detrimental and contain useful bioactive compounds. The increasing understanding of the biological and pharmacological significance of carbohydrates has helped to raise awareness of their importance in living systems and medicine, given they play numerous biological roles. For example, pectin has been identified as a class of secondary metabolites found in medicinal plants that may play a significant role in the treatment and management of a variety of NCDs. Pectin is mainly made of homogalacturonan, which is a linear polymer composed primarily of D-galacturonic acid units (at least 65%) linked in a chain by α-(1,4)-glycosidic linkages. There are also modified pectins or derivatives that improve pectin's bioavailability. Pectin is found in the cell walls of higher plants (pteridophytes, angiosperms, and gymnosperms), particularly in the middle lamella of the plant material. Citrus pectin is used in various industries. This article compiles information that has been available for years about the therapeutic importance of pectin in chronic diseases, different modes of pectin extraction, the chemistry of pectin, and the potency of pectin and its derivatives.

Enhancing pectin extraction from orange peel through citric acid-assisted optimization based on a dual response

Pectin is widely used in several products in the industry. Conventionally, strong and harmful acids are used for its extraction. This study optimized the extraction of orange peel's pectin using citric acid, considering yield and degree of esterification (DE) as response variables. Proximal analyses were performed, and the samples were subjected to a Box-Behnken design on three central points, considering as variables the temperature, time, and pH. The results of proximate analyses of the orange peels revealed 11.76 % moisture content, 87.26 % volatiles, 0.09 % ash, 50.45 % soluble carbohydrates, 70.60 % total carbohydrates, 0.89 % fixed carbon, 5.35 % lipids, and 36.75 mg GAE/g of phenolic compounds. The resulting second-order polynomial model described the relation of the input and output variables related to each other. The best performance to obtain a higher yield (18.18 %) of high methoxyl pectin (DE 50 %) was set at 100 °C/30 min/pH 2.48. Pectin showed antioxidant properties by ABTS and DPPH assays and similar thermal properties to the commercial polymer. Its equivalent weight was 1219.51 mol/g, and the methoxyl and anhydrouronic acid were 2.23 and 67.10 %, respectively. Hence, pectin extraction with citric acid results in a high-quality polymer and could be used as a gelling agent, stabilizer, or texturizer in food products.

The Effect of Opuntia ficus Mucilage Pectin and Citrus aurantium Extract Added to a Food Matrix on the Gut Microbiota of Lean Humans and Humans with Obesity.

Experimental studies have provided evidence that physicochemical interactions in the food matrix can modify the biologically beneficial effects of bioactive compounds, including their effect on gut microbiota. This work aimed to evaluate the effect of a food gel matrix with Opuntia ficus cladodes mucilage pectin and Citrus Aurantium extract on the growth of four beneficial gut bacteria obtained from the fecal microbiota of people who are lean or who have obesity after digestion in the upper digestive system. To accomplish this, a base formulation of Opuntia ficus cladodes mucilage with or without C. aurantium extract was submitted to an ex vivo fecal fermentation in an automatic and robotic intestinal system. The changes in the intestinal microbiota were determined by means of plate culture and 16S sequencing, while short-chain fatty acids (SCFA) produced in the colon were determined via gas chromatography. In the presence of the extract in formulation, greater growth of Bifidobacterium spp. (+1.6 Log10 Colonic Forming Unit, UFC) and Lactobacillus spp. (+2 Log10 UFC) in the microbiota of lean people was observed. Only the growth in Salmonella spp. (-1 Log10 UFC) from both microbiota was affected in the presence of the extract, which decreased in the ascending colon. SCFA was mainly produced by the microbiota of people who were lean rather than those who had obesity in the presence of the extract, particularly in the ascending colon. The effect of sour orange extract seems to depend on the origin of the microbiota, whether in people who have obesity (25 mM/L) or are lean (39 mM/L).

Deep eutectic solvent (DES)-assisted extraction of pectin from Ficus carica Linn. peel: optimization, partial structure characterization, functional and antioxidant activities.

The pectin from Ficus carica Linn. (fig) peels is a valuable and recyclable constituent that may bring huge economic benefits. To maximize the utilization of this resource, deep eutectic solvent (DES)-assisted extraction was applied to extract pectin from fig peels, and the extraction process was optimized with response surface methodology. When DES (choline chloride/oxalic acid = 1:1) content was 168.1 g kg-1, extraction temperature was 79.8 °C, liquid-solid ratio was 23.3 mL g-1, and extraction time was 120 min, the maximum yield of 239.6 g kg-1 was obtained, which was almost twice the extraction of hot water. DES-extracted fig peel pectin (D-FP) exhibited better nature than hot water-extracted fig peel pectin (W-FP) in terms of uronic acid content, particle size distribution, and solubility, but lower molecular weight and esterification degree. D-FP and W-FP had similar infrared spectra and thermodynamic peaks but differed in monosaccharide compositions. D-FP also showed good antioxidant capacities and exhibited better functional activities than W-FP. These results indicated that D-FP was of promising quality being utilized in food or medical industries and the optimal DES-assisted extraction method might be applied as a sustainable process for the effective extraction of bioactive pectin from fig peels with the excellence of low equipment requirements and simple operation. © 2024 Society of Chemical Industry.

Ultrasound-assisted alkali extraction of RG-I enriched pectin from thinned young apples: Structural characterization and gelling properties

RG-I (rhamnogalacturonan-I) pectin has recently gained growing interest owing to its diverse physiological activities. Nevertheless, exploration about its functional properties such as gelling and rheological characteristics is still lacking. In this study, RG-I enriched pectin was extracted from thinned young apples (Fuji) using an ultrasound-assisted alkaline method. The physical-chemical and gelling properties of the extracted pectin were investigated. The results revealed that thinned young apple pectin (TAP) was low methoxy pectin (LMP, DE = 17.94%), and rich in RG-I structural domain (86.5%). Moreover, TAP exhibited great thermal stability, high gel strength and water-holding capacity (90%). Scanning electron microscope (SEM) images demonstrated that TAP gel possessed a stable mesh-like structure characterized by small and densely packed voids. These findings provide valuable insights into the development of RG-I pectin gels and suggest that TAP extracted with the ultrasound-assisted alkaline method has significant potentials to be applied as gelling agents in the food industry.

Standardization of extraction of pectin from Assam lemon (Citrus limon Burm f.) peels using novel technologies and quality characterization

Standardization of extraction of pectin from Assam lemon (Citrus limon Burm f.) peels using novel technologies and quality characterization

The effect of different particle size reduction techniques on the biomass microstructure and the influence on the pectin extraction yield and structure

Particle size is known to be an influential parameter during pectin extraction, and a decreased particle size was already linked to an increased extraction efficiency. Particle size reduction can be achieved through different fracture mechanisms and this study investigated the effect of different particle size reduction techniques on the pectin extraction yield and structural characteristics. Three biomasses with an increasingly complex composition, being carrot pomace, broccoli stems and pumpkin pomace, were included in this study. For all three biomasses, high shear mixing, high pressure homogenization (HPH) and ball milling (BM) resulted in a significant decrease of the median particle diameter. After HPH and BM, samples with a comparable particle size distribution, but different microstructure, were obtained. After acid pectin extraction performed on these samples, a comparable and significantly higher extraction yield, compared to the non-treated sample, was obtained, indicating that particle size reduction resulted in an increased extraction efficiency. Yet, the fracture mechanism did not have a substantial influence. Overall, the different particle size reduction techniques had no clear impact on the structural characteristics of the extracted pectin, and the main difference between the different biomasses was the extent of protein and starch co-extraction during the pectin extraction process.

Environmentally Friendly Approach to Pectin Extraction from Grapefruit Peel: Microwave-Assisted High-Pressure CO2/H2O

In this research, pectin extraction from grapefruit peel (GPP) was performed using a microwave-assisted high-pressure CO2/H2O (MW-HPCO2) system. The Box–Behnken design of response surface methodology was applied for the optimization of MW-HPCO2 extraction conditions to obtain the highest pectin yield. The effects of temperature, time, and liquid/solid ratio on pectin yield were examined in the range of 100–150 °C, 5–15 min, and 10–20 mL g−1, respectively. Under the optimum extraction conditions (147 °C, 3 min, and 10 mL g−1), pectin was obtained with a yield of 27.53%. The results obtained showed that the extraction temperature and time had a strong effect on the pectin yield, while the effect of the liquid/solid ratio was not significant, and the pectin was effectively extracted from grapefruit peel (GP) using MW-HPCO2. Additionally, the application of GPP in apricot jam showed that MW-HPCO2-GPP can be used as a thickener in the food industry. The yield and physicochemical properties (ash, protein, galacturonic acid, reducing sugar and methoxyl content, degree of esterification, equivalent weight, color, viscosity) of pectin extracted in the optimum conditions of the MW-HPCO2 method were superior to pectin extracted by the traditional method. The results of this study revealed that MW-HPCO2 could be an innovative green and rapid technique for pectin extraction.

Clean Extraction of Pectin from Dragon Fruit Peels, Pomelo Peels, Okra, and Pineapple Peels Using Deep Eutectic Solvents and Ionic Liquids

Pectin is the main constituent of fruit peels that contributes to the fruit's solid and firm shape. Having wide applications in the food, pharmaceutical, and cosmetics industries, its extraction from bioresources would mark a sustainable advancement in biotechnology. The biomaterials for pectin extraction targeted in the study were dragon fruit peels (Hylocereus costaricensis), Pomelo peels (Citrus grandis), okra (Abelmoschus esculentus), and pineapple peels (Ananas comosus). Aqueous extractions of pectin from fruit peels were performed in a sono-reactor using deep eutectic solvents (DESs). Ionic liquids such as (Choline acetate ≥ 95%) [Ch][Ac] and (1-ethyl-3-methylimidazolium acetate ≥ 97%) [EMIM][Ac] were also employed as extraction solvents. Morphological screening with the electron microscope (SEM) and FTIR showed that the extracted pectin had a similar surface as commercial pectin. The extracted pectin can completely dissolve in water to form a homogenous suspension. The pectin yield from dragon fruit peels was 60±2.00 wt% with a degree of esterification at about 66-72%. This study introduces a clean extraction that can potentially substitute solvents in the pectin industry.

CHARACTERIZATION AND PHARMACEUTICAL EVALUATION OF DAUCUS CAROTA PECTIN AS A SUSPENDING AGENT IN ORAL DOSAGE FORMS

Background: Suspensions are equilibrated two-phased thermodynamically unstable systems increasingly used in the stability and bioavailability enhancement of a myriad of drug substances. Biodegradable, biocompatible and cost-effective biopolymers such as pectin are becoming popular alternative suspending agents to decrease sedimentation and ensure uniformity and accuracy in drug dosing. Nevertheless, limited commercial pectin sources with ideal gelling properties necessitate the search for novel sources such as carrots, a versatile vegetable. Objectives: The study therefore centred on the characterization and pharmaceutical evaluation of the suspending qualities of Daucus carota (carrot) pectin acquired from the conventional acid extraction procedure. Methodology: Fresh carrots were acquired, authenticated and processed before pectin extraction. The pectin extracted was characterized and evaluated for phytochemical properties such as proximate composition and degree of esterification. Different concentrations (1% and 2%) of carrot pectin and acacia gum (as a standard) were utilized in formulating paracetamol suspensions as suspending agents. Results: The pectin yield was 11.6% and contained glycosides, tannins, saponins, and phenols. FTIR analysis identified all the essential pectin functional groups while other characterization parameters showed quality high methoxyl pectin. Carrot pectin suspensions demonstrated significantly (P < 0.01) lower re-dispersibility and sedimentation rate when compared to acacia. The flow rate and sedimentation volumes were however non-significantly different. Conclusion: The characterization and suspending properties of carrot pectin have been successfully evaluated. Ultimately, acacia gum can be substituted with carrot pectin as a suspending agent.

Chemical and Pharmaceutical Features of Obtaining Pectins from Apple Pomace and Prospects for Use in Medicine and Pharmacy

The COVID-19 pandemic has shown the need to increase the level of providing patients with medicines. The development of new drugs using natural biomaterials remains relevant. The chemical and pharmaceutical features of obtaining apple pectin have been studied. The influence of technological factors during the extraction of apple pulp from different varieties of apples on the yield of pectin substances was studied. A chemical analysis of pectin content and output was carried out. The fractional composition of pectin substances was analyzed. Prospects for the use of pectins in medicine and pharmacy are outlined. The effect of the technological conditions of extraction of pectin extract from apple pomace on the content and yield of pectin in apple pulp from different varieties of apples was investigated. The technology for obtaining pectins from apple pulp in the presence of various acidic agents is presented, and it is established that the conditions for obtaining apple pectins are optimal for obtaining the highest yield of pectin compounds when choosing the technological parameters of the first stage of hydrolysis for summer and autumn varieties of apples using citric acid with a concentration of 0.10-0.15%, at a temperature of 80-85°C, lasting 2 hours for autumn varieties of apples, and 2.5 hours for summer varieties of apples. Under the conditions of the proposed technology, highly esterified apple pectin (degree of esterification on average 75%) was obtained from apple pulp of autumn and summer varieties of apples, which can be used in medicine and pharmacy.

Extraction of citrus pectin using pressurized carbon dioxide and production of its oligosaccharides

In this study, we utilized pressurized carbon dioxide (pCO2) for pectin extraction from citrus residues because of its potential to produce acid without chemicals, that is non-toxic, green technology for humans and the environment. The pCO2 was performed at 2.5 MPa and 90 °C for 90 min, while the conventional method (CM) for pectin extraction using HCl was performed under atmospheric pressure at 90 °C for 90 min. Pectin extraction yields with pCO2 depended on the type of citrus fruit and were lower than those obtained with the CM. However, pCO2-extracted pectin (pCO2-pectin) provided a higher degree of esterification (DE), neutral sugar side-chain substitution on the pectate backbone, and greater molecular size than those of pectin extracted using the conventional method (CM-pectin). Pectin oligosaccharide (POS) was prepared from CM-pectin and pCO2-pectin using viscozyme L hydrolysis under optimum conditions. These POSs were then designated as CM-POS and pCO2-POS, respectively. Both pCO2-POS and CM-POS stimulated the growth of Lactobacillus and Bifidobacterium species, and induced them to produce short-chain fatty acids, and slightly suppressed immunoglobulin E production in U266 cells. Furthermore, pCO2-POS had higher angiotensin-converting enzyme inhibitory activity compared to CM-POS. This study suggests that pCO2 is a novel method for extracting pectin with unique molecular properties affecting POS functionality in vitro.

Sequential extraction of anthocyanins and pectin from jabuticaba (Plinia cauliflora) peel: Peel pretreatment effect and ultrasound-assisted extraction.

The jabuticaba bark is rich in anthocyanins and fibers, and its use may be of industrial interest. In the food sector, its used as an ingredient in the production of fermented products, liqueurs or enriched flours. It also has pharmaceutical and cosmetic applications. The objective was to evaluate the effect of pretreatment and fresh use of jabuticaba peels in the extraction of total phenolic compound (TPC) and total anthocyanin (TA) contents with and without ultrasound assistance and in the sequential extraction of pectin from the residue. In the TPC and TA extraction, a 3x2 factorial design was used. For conventional anthocyanin extraction (CAE), occurred in an incubator under agitation. For ultrasound-assisted anthocyanin extraction (UAE) was utilized an ultrasonic homogenizer with probe (20 kHz, 160 W). The extracts were quantified (TPC, TA, antioxidant activity and color). The residues were characterized and used for sequential pectin extraction, which was quantified and characterized. The results were subjected to analysis of variance. Fresh jabuticaba peel is a residue that can be used to sequentially extract phenolic compounds, particularly anthocyanins and pectin. The use of ultrasound (UAE) was less efficient than CAE for extracting TPC and TA or performing sequential extraction on all pretreatment peels.

Extraction of pectin from acid lime peel

Extraction of pectin from acid lime peel

Optimization and characterization of pectin extracted from hawthorn by deep eutectic solvent

In this study, hawthorn pectin was extracted from dried hawthorn with deep eutectic solvent(DES) and compared with the traditional extraction methods such as acid extraction (AE) and ultrasonic-assisted extraction (UAE). Under optimal conditions, with a molar ratio of choline chloride to urea at 1:3, a water content of 30 %, a liquid-to-solid ratio of 30:1 (mL/g), an extraction temperature of 80 °C, an extraction time of 60 min, and a pH of 1, the yield of hawthorn pectin was 4.33 % ± 0.02 %. The measured results were consistent with the prediction. In addition, compared with AE and UAE, the experimental results showed that DES had a higher yield, a lower degree of esterification, and a slightly different monosaccharide composition from other extraction methods. The results of infrared spectroscopy and scanning electron microscopy showed that DES had a fine microstructure and coarser surface, and the main chemical structure of DES didn't change. The rheological analysis showed that DES had lower apparent viscosity than AE and UAE. These results represent a green source for pectin extraction with high pectin yield and good performance. In conclusion, the deep eutectic solvent has good application prospects in extracting hawthorn pectin.

Pectin extraction from Jackfruit waste by Acid extraction method

Pectin extraction from Jackfruit waste by Acid extraction method

Tropical Fruit Waste Management: Developing Pectin-based Biopolymer from Durian Rind (Durio zibethinus)

Durian (Durio zibethinus) is a popular native tropical fruit and is largely produced in Southeast Asia. In Indonesia, 1.71 million metric tons of durian were produced in 2022. However, this high production may lead to organic waste issues due to the large amount of durian rind generation. About 60 – 75% of the durian is the rind, which is mostly discarded, hard to be decomposed, and not utilized optimally. Meanwhile, the durian rind poses as a potential reservoir containing high concentrations of cellulose and pectin. Therefore, this research aimed to (1) extract pectin content from durian rind and (2) synthesize and characterize pectin-based biopolymer films. First, the pectin was extracted from dried durian rind powder using acid extraction and alcoholic precipitation methods. Then, biopolymer synthesis was done by mixing formulations of pectin, chitosan, ethylene glycol, and glycerol solutions to form thin films. About 0.13 – 0.71 g of pectin (0.46% of the pectin yield) was extracted per 100 g of dried durian rind powder. The FTIR spectroscopy results of the extracted pectin confirmed the presence of the O - H groups at 3327.83 cm-1, which indicated the pyranose ring, the main characteristic of pectin. Pectin-based biopolymer films were produced with thickness, transparency, and tensile strength of 0.01 – 0.07 mm, 7.40 - 40.50, and 1.26 – 2.69 MPa, respectively. The findings of the soil burial degradation test for biodegradability showed that the biopolymer experienced weight loss of up to 62% in 14 days. Considering the potential utilization of the durian rind for developing biopolymers as value-added bioproducts from agricultural biomass, tropical fruit waste management should be done properly in the future. This also can be offered as one of the alternative solutions for plastic substitution with proper development in the future.

Downstream Processing of Crude Ultrasound-Extracted Pectin From Saba Banana (Musa acuminata x balbisiana (BBB Group) "Saba") Peel.

Ultrasound-assisted extraction of pectin from Saba banana (Musa acuminata x balbisiana (BBB Group) "Saba") peels produced crude pectin that requires further purification. Two downstream processes (alcohol washing (AW) and acid demethylation (AD)) were compared. AW involved gelatinous precipitate washing with 85% alcohol and pressing to squeeze out liquids, while AD involved a sequential AW of the dried pectin with 60% acidified alcohol, and 60% and 95% alcohol solutions. Results showed that both methods produced low methoxyl pectins with similar color, yield, and moisture content, with no significant (p > 0.05) differences observed. AD, however, produced pectin with better quality in terms of ash content and anhydrouronic acid (AUA) content relative to the control. Fourier transform infrared spectra revealed that the samples contain -OH, C-H, COO-, COO, and C-O groups, but only AD has COO-R due to structural modification. Overall, AD has the potential to improve the quality of crude ultrasound-extracted pectin from Saba banana peels. Yet, pre-extraction processing methods are necessary to meet FAO color standards for pectin.