Bioaccessibility Of Pb In Soils Research Articles

Lead bioaccessibility and commonly measured soil characteristics in Detroit, Michigan

Historical and contemporary use of lead (Pb) in gasoline, paints, and industry have led to its concentration in urban soils, presenting a human health risk, with disproportionate impacts on children living in urban areas. Contaminated soil is one of the major contributors to child Pb exposure. While many residents practicing urban agriculture have their soil tested to determine the amount of soil total Pb present, this measure does not provide a direct indicator of soil Pb bioaccessibility (used to predict bioavailability). Because bioavailability is ultimately necessary to determine the exposure risk, we aimed to gain a more refined understanding of Pb risk using measurements commonly performed as part of typical agricultural soil tests. Accordingly, we sampled soils from 142 residential parcels located in Detroit, MI. Soils were evaluated for: pH, organic matter content, cation exchange capacity, phosphorus, sulfur, calcium, potassium, magnesium, and sodium and total and in-vitro bioaccessible (IVBA) Pb. Detroit soil IVBA Pb ranged from 4.5% to 81%, with a mean of 39%. Total Pb, organic matter (OM) content and soil pH were found to be the most important predictors of Pb bioaccessibility. Soils typically had a relatively low OM content (mean of 5%) however, samples with higher OM content had lower IVBA (e.g., the IVBA was 15.2% smaller in soils with 7.5% OM). Soils were typically slightly basic (mean of 7.8); however, samples with pH closer to neutral had lower IVBA (e.g., the IVBA was 11.6% smaller in soils with a pH of 7.3). The proximity of soil sampling locations to former smelter locations was also found to influence Pb bioaccessibility, with samples closer to former Pb smelter sites having a somewhat higher average IVBA. Results suggest increasing soil OM content in urban soils will reduce Pb bioaccessibility. Building soil OM would therefore improve both soil fertility and decrease soil Pb bioaccessibility, imparting a predicted decrease in exposure risks.

Successful Conversion of Pb-Contaminated Soils to Low-Bioaccessibility Plumbojarosite Using Potassium-Jarosite at Ambient Temperature.

Methods promoting lead (Pb) phase transformation in soils are essential for decreasing Pb bioaccessibility/bioavailability and may offer an in situ, cost-efficient process for mitigating contaminant exposure. Recent plumbojarosite (PLJ) conversion methods have shown the greatest potential to reduce soil Pb bioaccessibility, an in vitro bioaccessibility assay measurement of the proportion of Pb solubilized under gastric chemical conditions. Soils tested utilizing the recent PLJ method were found to have a Pb bioaccessibility of <1%, compared to original soils possessing bioaccessibility of >70%. However, this technique requires heat (95-100 °C) to promote mineral transformation. Jarosite-group minerals may incorporate multiple interlayer cations; therefore, we probed the potential for jarosite to remediate Pb via intercalation by reacting presynthesized potassium (K)-jarosite with aqueous Pb and/or Pb-contaminated soil at room temperature. Both K-jarosite and heated PLJ-treated samples were investigated by pairing bioaccessibility analyses with advanced bulk and spatially resolved X-ray absorption spectroscopy analyses. Samples treated with K-jarosite promoted Pb transformation to low-bioaccessibility (<10%) PLJ, with soil being converted to 100% PLJ using both heated and nonheated techniques. μ-X-ray fluorescence (μ-XRF) and μ-X-ray absorption near-edge structure (μ-XANES) showcase significant differences between elemental interactions for heated and nonheated PLJ-treated samples with anglesite impurities being found on the microscale. Although further development is necessary to accommodate for suitable field conditions, results indicate, for the first time, that K-jarosite may successfully convert soil Pb to PLJ without high-temperature conditions. The newfound utility of K-jarosite is expected to be key to future jarosite-based soil Pb remediation method development.

The effects of biochar and redox conditions on soil Pb bioaccessibility to people and waterfowl

Biochar can reduce lead (Pb) bioavailability to plants in metal-contaminated soil, but the ability of biochar to reduce the bioavailability of soil Pb to people and wildlife remains unknown. In this study, 17 biochars were evaluated as in situ amendments for three soils with distinct sources of Pb contamination (smelter emissions, ceramics waste, mining waste), hydrology (upland, well-drained soil vs submerged wetland soil), and biological receptors (human vs waterfowl). Biochars were made from blends of 30% manure (poultry litter or dairy manure) and 70% lignocellulosic material (wheat straw or grand fir shavings) and pyrolyzed at 300, 500, 700, and 900 °C. Soils were amended with 2% biochar (w/w) and incubated for 6 months. A suite of standard (e.g., EPA Method 1340) and experimental soil Pb bioaccessibility assays were used to assess the impact of the treatments. The results showed that biochar amendments to upland soils resulted in modest reductions in gastrointestinal Pb bioaccessibility (maximum reduction from 78 to 68% bioaccessibility as a percent of total, EPA Method 1340 at pH 2.5). In the wetland soil, sample redox status had a greater impact on Pb bioaccessibility than any amendment. Low-solubility Pb sulfides in this soil oxidized over the course of the study and no treatment was able to offset the increase in Pb bioaccessibility caused by this oxidation. The impact of redox status on Pb bioaccessibility was only evident when soil bioaccessibility assays were adapted to preserve sample redox status. This result highlights the importance of maintaining in situ redox conditions when processing/analyzing samples from low-oxygen environments and that soil remediation efforts should consider the role of redox conditions on Pb bioaccessibility.

Correlating soil nutrient test lead with bioaccessible lead in highly-contaminated soils receiving lead-immobilizing amendments

Lead (Pb) is one of the most common metals exceeding human health risk guidelines for soil concentrations worldwide. Pb bioaccessibility is known to vary depending on soil physiochemical characteristics and, as a result, in vitro and in vivo tests exist that are used to estimate bioaccessible Pb in contaminated soils. Although in vitro tests such as the relative bioaccessibility leaching procedure (RBALP) present simpler and more cost-effective risk assessments than in vivo methods, soil tests such as Mehlich-3, Modified Morgan, and ammonium bicarbonate-diethylenetriamine pentaacetate (AB-DTPA) extractions are extremely routine and even more cost-effective. Currently, there are few comparisons examining the viability of common soil nutrient tests for assessing Pb bioaccessibility in soils from contaminated sites with extremely high total Pb concentrations or for sites that have received amendments, such as those containing compost, iron, and/or phosphorus, intended to immobilize Pb. Here, we examine the correlation between RBALP Pb and Pb as determined using three commonly utilized soil tests, Mehlich-3, Modified Morgan, and AB-DTPA, in archived samples from one Pb-contaminated site receiving compost amendment (Seattle, WA, USA) and one extremely Pb-contaminated site receiving mixtures of compost, P, and Fe (Joplin, MO, USA). At both the Seattle and Joplin sites separately, RBALP Pb was significantly correlated with all three soil nutrient test values, regardless of soil amendment. However, RBALP was only significantly correlated with Modified Morgan and total Pb when examining the Joplin and Seattle data together, likely resulting from different factors controlling Pb solubility at the two sites. These findings suggest that a diverse suite of relatively inexpensive and accessible soil nutrient test methods correlate with bioaccessible Pb at a specific site, regardless of whether Pb-immobilizing amendments have been used.

Phytostabilization of a contaminated military site using Miscanthus and soil amendments.

Military activities can contaminate productive land with potentially toxic substances. The most common trace metal contaminant on military bases is lead (Pb). A field experiment was begun in 2016 at Fort Riley, KS, in an area with total soil Pb concentrations ranging from 900 to 1,500mg kg-1 and near-neutral pH. The main objectives were to test the potential of Miscanthus sp. for phytostabilization of the site and to evaluate the effects of soil amendments on Miscanthus growth, soil-plant Pb transfer, bioaccessibility of soil Pb, and soil health. The experimental design was a randomized complete block, with five treatments and four replications. Treatments were (a) existing vegetation; (b) Miscanthus planted in untilled soil, no amendments; (c) Miscanthus planted in tilled soil; (d) Miscanthus planted in tilled soil amended with inorganic P (triple superphosphate applied at 5:3Pb:P); and (e) Miscanthus planted in tilled soil amended with organic P (Class B biosolids applied at 45 Mg ha-1 ). Tilling and soil amendments increased dry matter yields only in the establishment year. Total Pb uptake, plant tissue Pb concentration, and soil Pb bioaccessibility were significantly less in the Miscanthus plots amended with biosolids than the Miscanthus plots with no added P across all 3 yr. Enzyme activities, organic carbon, and microbial biomass were also greater in biosolids-treated plots. Results show that planting-time addition of soil amendments to Pb-contaminated soil supported Miscanthus establishment, stabilized and reduced bioaccessibility of soil Pb, reduced concentration and uptake of Pb by Miscanthus, and enhanced soil health parameters.

Assessment of human health risk due to lead in urban park soils using in vitro methods

Beijing parks always have a large flow of local residents and tourists, and the soil Pb could threaten human health by incidental ingestion. Soil samples from eleven parks in Beijing were collected to assess the human health risk associated with Pb. Lead bioaccessibility in these parks ranged from 3.2 ± 0.4% to 12.1 ± 0.5% in the physiologically based extraction test (PBET) gastric phase and increased when approaching the city center. The chemical forms and soil properties (Fe, organic matter, and grain size) were important factors affecting the soil Pb bioaccessibility. The geo-accumulation index of Beihai Park (BH, near the city center) reached 1.3 ± 0.1 indicating moderate contamination. Lead health risk to children in BH should be of concern though its hazard quotient was below one. Results obtained from the Diffusive Gradients in Thin-films (DGT)-induced fluxes in the soils (DIFS) model showed that Pb-release in some parks farther from the city center was a “partially sustained case” (Rdiff < R < 0.95) indicating that soil particles could partially replenish effective Pb to the soil solution. A relatively higher desorption rate constant (k1) and shorter characteristic response time (Tc) were also found in these parks, indicating non-negligible release risk. Soil Pb based on the PBET method and DIFS model could provide a reliable reference to park managers for the health risk management of Pb pollution.

Effect of dietary vitamins in oral bioaccessibility of lead in contaminated soils based on the physiologically based extraction test

To determine the effect of vitamin supplements on the oral bioaccessibility of Pb in soils, Pb bioaccessibility was measured in the presence of 9 vitamins by a physiologically based extraction test. Gastric Pb bioaccessibility (G-BA, 2.6–83.3%) was found to be mostly reduced (1.1–3.1 fold) in the presence of B vitamins, specifically vitamins B1, B6, and B9. In contrast, a significant increase in Pb G-BA was observed with vitamin C and E involved. In the small intestinal phases, Pb bioaccessibility (I-BA) ranged from 0.1% to 16.0%, being 5–50 fold lower than the corresponding G-BA values. Vitamin C supplementation showed a 7-fold increase in Pb I-BA, with a similar increase presented in approximately 30% of samples treated to vitamin B involvement. Lead liberation in gastrointestinal digests was associated with the dissolution of Fe and Mn regulated by vitamins. In conclusion, the addition of B vitamins resulted in the reduction of gastric Pb bioaccessibility, but the bioaccessibility value increased in participation of vitamin C and E. Elevated intestinal bioaccessibility was found especially for vitamin C. This should contribute to more accurate assessment of health risks from contaminated soils. Nutritional management aimed at preventing Pb-induced toxicity can benefit from knowledge of vitamin influence on soil Pb bioaccessibility.

Lead bioaccessibility in farming and mining soils: The influence of soil properties, types and human gut microbiota

To better understand the risk assessment of Lead (Pb) in contaminated soils, 78 soil samples were collected from different locations in China and Pb bioaccessibility was assessed using the PBET (The Physiologically Based Extraction Test) method combined with SHIME (The Simulator of the Human Intestinal Microbial Ecosystem), and Pb bioaccessibility data from the PBET method on 88 soil samples that found in the literature were also used for the assessment. For all the soils, the mean Pb bioaccessibility was as follows: the gastric phase (31.25%) > colon phase (17.78%) > small intestinal phase (10.13%). The values of Pb bioaccessibility in most soils were lower than 60%, which is the typical default assumption for Pb (RBA, relatively bioavailability) by the US EPA. Mean Pb bioaccessibility (41.10% and 14.00% for gastric and small intestinal phases, respectively) in the present study was slightly higher than the values from the literature (24.80% and 8.68% for gastric and small intestinal phases, respectively) in the gastrointestinal tract. Mean Pb bioaccessibility was lower in acidic soil during the small intestinal phase, while the values for the alkaline soil were higher in the small intestinal and colon phases. In the gastric and small intestinal phases, mean Pb bioaccessibility in farming soils was slightly lower than it was in mining soils. However, the mean Pb bioaccessibility from farming soils was increased compared with mining soils in the colon phase given the action of human gut microbiota. Soil pH and type are important factors for predicting soil Pb bioaccessibility and health risk.

Investigating Lead Species and Bioavailability in Contaminated Soils: Coupling DGT Technique with Artificial Gastrointestinal Extraction and in Vivo Bioassay.

Although strong in vivo-in vitro correlations (IVIVCs) between relative bioavailability (RBA) and bioaccessibility of soil Pb were well reported, knowledge on the fractions of bioaccessible Pb in simulated gastrointestinal (GI) fluids that are available for absorption into the systemic circulation is limited. Here, Pb-RBA in 14 Pb-contaminated soils were assessed using an in vivo mouse bioassay and compared to Pb bioaccessibility by the gastrointestinal phase of the UBM (Unified Bioaccessibility research group of Europe (BARGE) Method) in vitro assay with and without 0.45 μm filtration of GI fluid. Results showed good IVIVC between Pb-RBA and Pb bioaccessibility without filtration ( r 2 = 0.62), while Pb bioaccessibility with filtration provided a poor correlation with Pb-RBA ( r 2 = 0.16). This suggested that besides dissolved Pb ions, Pb-complexes formed in the UBM gastrointestinal fluid might also contribute to bioavailable Pb. To ascertain this, DGT (diffusive gradients in thin-films) devices which can measure both Pb2+ ions and labile inorganic and organic Pb-complexes were introduced to the UBM fluids to measure Pb DGT-bioaccessibility, which showed strong correlation to Pb-RBA ( r 2 = 0.71). With increasing diffusive gel thickness which could enhance release of Pb ions from Pb-complexes, Pb DGT-bioaccessibility increased by 3.4-5.7 times, while inclusion of dialysis membrane within DGT devices significantly decreased Pb DGT-bioaccessibility by inhibiting diffusion of Pb complexes to binding gel. These results confirmed the contribution of Pb-complexes to Pb bioavailability, providing new insights to Pb bioavailability.

The source of lead determines the relationship between soil properties and lead bioaccessibility

Lead (Pb) contaminated soil is of particular concern for infants and children due to their susceptibility to exposure, fast metabolic rates and rapidly developing neuronal systems. Determining the bioaccessibility of Pb in soils is critical in human health risk assessments, which can vary due to different soil properties and sources of Pb contamination. In this study, the potential relationships between soil properties and Pb bioaccessibility from various Pb sources including Pb contamination from mining (specifically, Broken Hill), three shooting ranges, a smelter and two industry sites (pottery and battery), were investigated using the Relative Bioavailability Leaching Procedure (RBALP). We found the following: (1) CEC, TOC, sand and silt content, and total Pb were significantly different (p < 0.05) between the two particle size fractions of < 2 mm and < 250 μm; (2) EC, CEC and total Pb were significantly correlated to Pb bioaccessibility (p < 0.05); and (3) soil analyses based on source of Pb demonstrated a strongly significant relationship between Pb bioaccessibility and soil properties (CEC, EC, clay content and total Pb) for mining soils from Broken Hill (r2 = 0.86, p < 0.05, n = 18). These results demonstrated the influences of Pb contamination sources, soil properties and particle size fractions on Pb bioaccessibility as well as the prediction of Pb bioaccessibility using soil properties. The findings documented here will help in developing a predictive tool for human health risk assessment and the remediation of Pb contaminated soils.

Use of Routine Soil Tests to Estimate Pb Bioaccessibility.

Soil lead (Pb) hazard level is contingent on bioavailability, but existing assays that estimate Pb bioavailability for human health risks are too expensive or otherwise inaccessible to many people that are impacted by Pb-contaminated soil. This study investigated the use of routine soil nutrient tests to estimate soil-Pb bioaccessibility as a surrogate measure of Pb bioavailability. A silt loam soil was spiked to a target concentration of 2000 mg Pb kg-1 with Pb(NO3)2 and amended with H3PO4 (varying P-to-Pb molar ratios) and KCl (Cl-to-P molar ratio of 2:5) to generate soils with similar total Pb concentrations but a range of Pb bioavailability (and bioaccessibility). Soils were extracted using Mehlich 3, Mehlich 1, Bray P1, Olsen, andmicronutrient (DTPA) methods, and the results were compared to U.S. Environmental Protection Agency method 1340 data as well as to extended X-ray absorption fine structure (EXAFS) spectroscopy. The Mehlich 3 and method 1340 treatment effect ratios were well-correlated ( r2 = 0.88, p ≤ 0.05), whereas Bray P1, DTPA, and Olsen results were more reflective of EXAFS data. Preliminary animal-feeding trials suggest that the Mehlich 3 is as effective as method 1340 at predicting the impact of P treatment on Pb relative bioavailability; however, both methods over-estimated the Pb hazard to mice in P-amended soil. Other routine soil tests that have heightened sensitivity to P amendment (e.g., Bray P1) may be promising candidates for Pb bioaccessibility assessment.

Estimates of potential childhood lead exposure from contaminated soil using the US EPA IEUBK Model in Sydney, Australia

Surface soils in portions of the Sydney (New South Wales, Australia) urban area are contaminated with lead (Pb) primarily from past use of Pb in gasoline, the deterioration of exterior lead-based paints, and industrial activities. Surface soil samples (n=341) were collected from a depth of 0–2.5cm at a density of approximately one sample per square kilometre within the Sydney estuary catchment and analysed for lead. The bioaccessibility of soil Pb was analysed in 18 samples. The blood lead level (BLL) of a hypothetical 24 month old child was predicted at soil sampling sites in residential and open land use using the United States Environmental Protection Agency (US EPA) Integrated Exposure Uptake and Biokinetic (IEUBK) model. Other environmental exposures used the Australian National Environmental Protection Measure (NEPM) default values. The IEUBK model predicted a geometric mean BLL of 2.0±2.1µg/dL using measured soil lead bioavailability measurements (bioavailability =34%) and 2.4±2.8µg/dL using the Australian NEPM default assumption (bioavailability =50%). Assuming children were present and residing at the sampling locations, the IEUBK model incorporating soil Pb bioavailability predicted that 5.6% of the children at the sampling locations could potentially have BLLs exceeding 5µg/dL and 2.1% potentially could have BLLs exceeding 10µg/dL. These estimations are consistent with BLLs previously measured in children in Sydney.

Pollution and Oral Bioaccessibility of Pb in Soils of Villages and Cities with a Long Habitation History.

The Dutch cities Utrecht and Wijk bij Duurstede were founded by the Romans around 50 B.C. and the village Fijnaart and Graft-De Rijp around 1600 A.D. The soils of these villages are polluted with Pb (up to ~5000 mg/kg). Lead isotope ratios were used to trace the sources of Pb pollution in the urban soils. In ~75% of the urban soils the source of the Pb pollution was a mixture of glazed potsherd, sherds of glazed roof tiles, building remnants (Pb sheets), metal slag, Pb-based paint flakes and coal ashes. These anthropogenic Pb sources most likely entered the urban soils due to historical smelting activities, renovation and demolition of houses, disposal of coal ashes and raising and fertilization of land with city waste. Since many houses still contain Pb-based building materials, careless renovation or demolition can cause new or more extensive Pb pollution in urban soils. In ~25% of the studied urban topsoils, Pb isotope compositions suggest Pb pollution was caused by incinerator ash and/or gasoline Pb suggesting atmospheric deposition as the major source. The bioaccessible Pb fraction of 14 selected urban soils was determined with an in vitro test and varied from 16% to 82% of total Pb. The bioaccessibility appears related to the chemical composition and grain size of the primary Pb phases and pollution age. Risk assessment based on the in vitro test results imply that risk to children may be underestimated in ~90% of the studied sample sites (13 out of 14).

Bioaccessibility of lead in urban soil of Broken Hill, Australia: A study based on in vitro digestion and the IEUBK model

This study was conducted to investigate lead (Pb) bioaccessibility in urban soil and to assess health risk to children in the city of Broken Hill, Australia, which was established around one of the world's largest lead–zinc–silver mines. Fifty-three topsoil (0–0.1m) and 50 subsoil (0.3–0.5m) samples were collected from earthen footpaths, nature strips, parks or vacant land throughout the urban area. The soil samples were analysed for total Pb concentration, Pb bioaccessibility and Pb mineral phases, together with important soil physicochemical properties known to influence Pb bioaccessibility. Lead bioaccessibility ranged from 24% to 89% in topsoil and from 16% to 100% in subsoil, exhibiting a generally decreasing pattern with increasing distance from the orebody. Lead bioaccessibility was strongly positively related to total Pb concentration in both the topsoil and subsoil. In subsoil, a moderate negative correlation existed between Pb bioaccessibility and soil pH, while a moderate positive correlation existed between Pb bioaccessibility and soil organic matter (OM) content. In contrast, only a weak positive correlation existed between Pb bioaccessibility and OM content in topsoil. The presence of different Pb mineral phases also appeared to have caused variation in soil Pb bioaccessibility, with galena (PbS)-rich samples tending to exhibit lower Pb bioaccessibility. The prediction of blood lead (PbB) levels in Broken Hill children aged 1–4years using the IEUBK model well matched the measured data from a recent PbB screening, suggesting a high risk of childhood chronic low-level Pb exposure (PbB levels >5μg/dL) in Broken Hill, especially in the vicinity of the orebody. Future Pb abatement programs in Broken Hill should utilise the IEUBK model to establish target soil Pb values in an effort to achieve particular child PbB outcomes.

Potential bioavailability of lead, arsenic, and polycyclic aromatic hydrocarbons in compost-amended urban soils.

Urban soils may contain harmful concentrations of contaminants, such as lead (Pb), arsenic (As), and polycyclic aromatic hydrocarbons (PAHs), that can transfer from soil to humans via soil ingestion and consumption of food crops grown in such soils. The objective of this research was to assess the effectiveness of adding different compost types to reduce both direct (soil-human) and indirect (soil-plant-human) exposure of Pb, As, and PAHs to humans. A field experiment was conducted in 2011 and 2012 at an urban garden site with elevated concentrations of Pb (475 mg kg), As (95 mg kg), and PAHs (23-50 mg kg). Soil amendments were composted biosolids, noncomposted biosolids, mushroom compost, leaf compost, and a nonamended control. Collard greens, tomatoes, and carrots were then grown in the amended and nonamended soils and nonamended soils that received urea in 2011. At the beginning of the second season, N-P-K fertilizer was added to all plots. The potential for direct and indirect exposure was evaluated. Soil Pb bioaccessibility was 1 to 4.3%, and As bioaccessibility was 7.3 to 12.3%. Composted biosolids reduced the bioaccessibility of soil Pb by ∼17% compared with the control but temporarily increased the bioaccessibility of As by ∼ 69% compared with the control when soluble inorganic P concentration in soil was elevated by P fertilizer application in 2012. The bioaccessibility of soil Pb decreased by ∼38% in all treatments when soluble inorganic P concentration in soil was elevated by P fertilizer. Compost amendments reduced the concentrations of low molecular weight PAHs in soil. Regardless of the treatments, the concentrations of Pb, As, and PAHs measured in the vegetables were low or nondetectable, except for Pb in carrots. Consumption of vegetables grown at this site will cause insignificant transfer of Pb, As, and PAHs to humans.

Safety of gardening on lead- and arsenic-contaminated urban brownfields.

Elevated levels of lead (Pb) and arsenic (As) are not uncommon for urban soils. Test plots were established at urban gardens in Tacoma and Seattle, WA. The Tacoma site was contaminated with Pb (51-312 mg kg) and As (39-146 mg kg), and the Seattle site had high Pb soil concentrations ranging from 506 to 2022 mg kg and As concentrations of <20 mg kg. The efficacy of biosolids mix and compost amendment in reducing Pb and As concentrations in three vegetables (carrots, lettuce, and tomatoes) and the bioaccessibility of soil Pb and As were evaluated. Food-chain transfer of Pb and As were evaluated by measuring plant Pb and As concentrations after kitchen-style washing, a laboratory cleaning procedure, or peeling. The experimental design was a randomized complete block with a split-plot arrangement. Tacoma site treatments included a Class A biosolids mix (TAGRO) with dolomite, and soil at the Seattle site was amended with Cedar-Grove compost (CGC) plus dolomite. TAGRO amendment diluted soil Pb by 10 to 23% and As by 12 to 25% at the Tacoma site, and CGC + dolomite resulted in 20 to 50% dilution in soil Pb at the Seattle site. Both amendments reduced Pb concentrations in vegetables by 50 to 71%, and As reductions ranged from 46 to 80%. At the Tacoma site, Pb concentrations (dry weight basis) in carrots, lettuce, and tomatoes ranged from 8.89 to 25.0, from 0.37 to 3.83, and from 0.54 to 1.24 mg kg, respectively. Plant As concentrations were below 703 μg kg (dry weight) for the vegetables and followed the order lettuce > carrot > tomato. Food-chain transfer of Pb and As in vegetables grown in contaminated urban soils were reduced by laboratory cleaning.

Field evaluations on soil plant transfer of lead from an urban garden soil.

Lead (Pb) is one of the most common contaminants in urban soils. Gardening in contaminated soils can result in Pb transfer from soil to humans through vegetable consumption and unintentional direct soil ingestion. A field experiment was conducted in 2009 and 2010 in a community urban garden with a soil total Pb concentration of 60 to 300 mg kg. The objectives of this study were to evaluate soil-plant transfer of Pb, the effects of incorporation of a leaf compost as a means of reducing Pb concentrations in vegetables and the bioaccessibility of soil Pb, and the effects of vegetable cleaning techniques on the Pb concentrations in the edible portions of vegetables. The amount of compost added was 28 kg m. The tested plants were Swiss chard, tomato, sweet potato, and carrots. The vegetable cleaning techniques were kitchen cleaning, laboratory cleaning, and peeling. Compost addition diluted soil total Pb concentration by 29 to 52%. Lead concentrations of the edible portions of vegetables, except carrot, were below the maximum allowable limits of Pb established by the Food and Agriculture Organization and the World Health Organization. Swiss chard and tomatoes subjected to kitchen cleaning had higher Pb concentrations than laboratory-cleaned plants. Cleaning methods did not affect Pb concentrations in carrots. Bioaccessible Pb in the compost-added soils was 20 to 30% less than that of the no-compost soils; compost addition reduced the potential of transferring soil Pb to humans via vegetable consumption and direct soil ingestion. Thorough cleaning of vegetables further reduced the potential of transferring soil Pb to humans.

Amending Soils With Phosphate As Means To Mitigate Soil Lead Hazard: A Critical Review Of The State Of The Science

Ingested soil and surface dust may be important contributors to elevated blood lead (Pb) levels in children exposed to Pb contaminated environments. Mitigation strategies have typically focused on excavation and removal of the contaminated soil. However, this is not always feasible for addressing widely disseminated contamination in populated areas often encountered in urban environments. The rationale for amending soils with phosphate is that phosphate will promote formation of highly insoluble Pb species (e.g., pyromorphite minerals) in soil, which will remain insoluble after ingestion and, therefore, inaccessible to absorption mechanisms in the gastrointestinal tract (GIT). Amending soil with phosphate might potentially be used in combination with other methods that reduce contact with or migration of contaminated soils, such as covering the soil with a green cap such as sod, clean soil with mulch, raised garden beds, or gravel. These remediation strategies may be less expensive and far less disruptive than excavation and removal of soil. This review evaluates evidence for efficacy of phosphate amendments for decreasing soil Pb bioavailability. Evidence is reviewed for (1) physical and chemical interactions of Pb and phosphate that would be expected to influence bioavailability, (2) effects of phosphate amendments on soil Pb bioaccessibility (i.e., predicted solubility of Pb in the GIT), and (3) results of bioavailability bioassays of amended soils conducted in humans and animal models. Practical implementation issues, such as criteria and methods for evaluating efficacy, and potential effects of phosphate on mobility and bioavailability of co-contaminants in soil are also discussed.

High‐Iron Biosolids Compost–Induced Changes in Lead and Arsenic Speciation and Bioaccessibility in Co‐contaminated Soils

The safety of urban farming has been questioned due to the potential for contamination in urban soils. A laboratory incubation, a field trial, and a second laboratory incubation were conducted to test the ability of high-Fe biosolids-based composts to reduce the bioaccessibility of soil Pb and As in situ. Lead and As bioaccessibility were evaluated using an in vitro assay. Changes in Pb, As, and Fe speciation were determined on select samples after the second laboratory incubation using μ-X-ray fluorescence mapping followed by μ-X-ray absorption near-edge structure (XANES). A compost with Fe added to wastewater treatment residuals (Fe WTR compost) added to soils at 100 g kg decreased Pb bioaccessibility in both laboratory incubations. Mixed results were observed for As. Composts tested in the field trial (Fe added as Fe powder or FeCl) did not reduce bioaccessible Pb, and limited reductions were observed in bioaccessible As. These composts had no effect on Pb bioaccessibility during the second laboratory incubation. Bulk XANES showed association of Pb with sulfates and carbonates in the control soil. μ-XANES for three points in the Fe WTR amended soil showed Pb present as Fe-sorbed Pb (88 and 100% of two points) and pyromorphite (12 and 53% of two points). Bulk XANES of the Fe WTR compost showed 97% of total Fe present as Fe. The results of this study indicate that addition of high-Fe biosolids compost is an effective means to reduce Pb accessibility only for certain types of Fe-rich materials.

Modelling Pb bioaccessibility in soils contaminated by mining and smelting activities

This investigation was undertaken to identify the most significant parameters that can be used to model the bioaccessibility of lead in contaminated soils. Twenty-five soil samples contaminated by two distinct sources of contamination (mining and smelting areas) were investigated in this work. Edaphic soil properties were determined using the classic techniques. Speciation and solid phase distribution of Pb were determined by sequential fractionation and physical analysis (XRD and SEM-EDX). Bioaccessibility of Pb was measured using the unified BARGE protocol on each soil samples and each residue following the different steps of the sequential extraction procedure. Results showed that Pb speciation varied according to the source of contamination. Pb gastric bioaccessibility ranged from 12% to 86% in mining soils and from 40% to 95% in smelting soils. Soil edaphic properties (pH, CEC, clays content or organic matter content) cannot be used to model Pb bioaccessibility as these parameters could not be extrapolated from on site to another. The differences in Pb bioaccessibility between the 25 soils were better reflected by the nature of the Pb-bearing minerals as characterized by sequential extractions. Soluble, exchangeable and acid-soluble phases could explain from 70 to 98% of Pb bioaccessibility. Consequently, Pb bioaccessibility is extremely sensitive to the Pb-bearing phase distribution. This distribution should be thoroughly evaluated before applying any remedial techniques that would potentially affect Pb speciation in soils.