Abstract Transpiration (T), the component of evapotranspiration (ET) controlled by the vegetation, dominates terrestrial ET in many ecosystems; however, estimating it accurately, especially at the global scale, remains a considerable challenge. Existing approaches mostly rely on the relationship between T and photosynthesis, but untangling this relationship is difficult and leads to diverging T estimates. Limited in-situ measurements and the inability to directly measure transpiration from space further complicate the reliable assessment of this crucial process in the terrestrial water cycle. Here, we developed a new hybrid Priestley-Taylor (PT) model combined with an Artificial Neural Network (ANN) using globally available remote sensing and reanalysis data of soil moisture, vapor pressure deficit and windspeed. We also take advantage of the newly released global sap flow measurement network SAPFLUXNET. In the proposed approach, we avoid the parameterization of stomatal conductance by training the ANN on the PT-Coefficient α, obtained by inverting the PT equation. The results showed that our model framework can estimate T in different forest ecosystems based on few predictors. By utilizing forcings from independent datasets, we eliminate the reliance on in-situ measurements for predicting T. Through upscaling actual observations to a larger scale, this model framework helps alleviate the scarcity of T products. Intercomparison of T with ET partitioning methods based on eddy covariance data, shows high performances (KGE of 0.69 in Europe and 0.60 in North America), slightly improving estimates compared to other models. Analysis of contribution of T to ET across 100 FLUXNET sites result in a global mean of 55.2%. We believe that modelling T independent from the carbon cycle can support our understanding of land-atmosphere feedbacks and climate extremes in future research.