Erythrocyte Parameters Research Articles

Effects of the laying on of hands on anxiety, stress and autonomic response of employees in a hospital: A double-blind randomized controlled trial

Mental and behavioral disorders result in increased absenteeism and abandonment of work. The objective of this study was to evaluate the anxiety, stress, depression, negative and positive affects, and hematological and autonomic responses of employees in a public hospital exposed to laying on of hands with (LHS) or without Spiritual connection (control-LHW). MethodologyEighty-four employees with anxiety/stress were enrolled and randomly allocated into two groups of intervention LHS by Spiritist “passe” and control-LHW by volunteers with healing intent. Anxiety, stress and depression were evaluated by Depression Anxiety and Stress Scale (DASS21). Negative and positive affects by Subjective Well-being Scale (SWS), cardiac autonomic modulation by heart rate variability and cytokines and blood count were assessed by blood sample. ResultsOur study showed a significant improvement in the neutrophils (p = 0.041; d = 0.70) and cardiac parasympathetic activity, and reduction in VLF (p < 0.005) in the LHS. There was reduction in erythrocyte parameters and interleukin 10 (p < 0.005) in the control-LHW. In both groups there was a significant reduction in anxiety, stress, depression and negative affects after one session (p < 0.005). ConclusionLaying on of hands with Spiritual connection by Spiritist “passe” appears to be effective in increasing cardiac vagal activity and regulation of immune responses of employees in a public hospital with stress/anxiety.

Hemoglobin structure at higher levels of hemoglobin A1C in type 2 diabetes and associated complications.

Background:Fourier transform infrared (FTIR) spectroscopy technique has not been used as a diagnostic tool for diabetes in clinical practice. This study was linked to structural changes in hemoglobin (Hb) in type 2 diabetes patients at higher levels of HbA1C using FTIR spectroscopy.Methods:Fifty-three diabetic patients from the Bahawal Victoria Hospital, Bahawalpur, Pakistan were categorized as group A (6% < HbA1C < 7%; n = 25) and group B (HbA1C ≥9%; n = 28). Another group (group N) of twenty blood samples was taken from healthy people from the Islamia University Bahawalpur, Pakistan. Data from all groups were collected from January 1, 2018 to March 31, 2019. The structure of Hb was studied by FTIR spectroscopy and impact of glucose on the fine structure of HbA1C was estimated.Results:Hb secondary structure erythrocyte parameters were altered by changing glucose concentrations. From FTIR spectra of all three groups it was found that Hb structure was slightly altered in group A, but significantly changed in group B (P < 0.05). There was an increase in β-sheet structure and a reduction in α-helix structure at elevated levels of HbA1C (group B) in type 2 diabetes.Conclusion:We suggest that higher level of glycation reflected by increased HbA1C might be a contributing factor to structural changes in Hb in type 2 diabetic patients. FTIR spectroscopy can be a novel technique to find pathogenesis in type 2 diabetes.

Comprehensive hematological reference intervals in a healthy adult male population

Reference intervals (RIs) are important tools for improving medical decision-making. Hematology reference values can be influenced by important covariates such as genetic and environmental factors, rendering it essential to define RIs for specific populations. Therefore, we aimed to establish accurate and robust RIs for hematological markers in a healthy adult male Iranian population. This cross-sectional study was conducted in a population of 723 males aged 20-60 years old. Hematological parameters were routinely measured using a Sysmex auto analyser system (KX-21 N). The quality of assays was monitored using commercial quality control samples. The nonparametric rank method, as recommended by the Clinical and Laboratory Standards Institute (CLSI) guidelines, was used to calculate the 2.5th and 97.5th percentiles as the lower and upper reference limits, respectively. Of the 12 hematological parameters assessed, only mean platelet volume (MPV) demonstrated significant age-specific differences, requiring two partitions from 20 to 35 years (8.7-12.2 fL) and 35 to 65 years (8.5-11.5 fL). The remaining hematological parameters (e.g. leukocyte, erythrocyte, and platelet parameters) could be defined by one age range. This study established RIs for 12 routinely used hematological parameters in a healthy male population living in the northeastern region of Iran. Established RIs differed from those previously reported by other cohorts, highlighting the importance of population-specific RIs.

Prediction of hypertension, hyperglycemia and dyslipidemia from retinal fundus photographs via deep learning: A cross-sectional study of chronic diseases in central China

Retinal fundus photography provides a non-invasive approach for identifying early microcirculatory alterations of chronic diseases prior to the onset of overt clinical complications. Here, we developed neural network models to predict hypertension, hyperglycemia, dyslipidemia, and a range of risk factors from retinal fundus images obtained from a cross-sectional study of chronic diseases in rural areas of Xinxiang County, Henan, in central China. 1222 high-quality retinal images and over 50 measurements of anthropometry and biochemical parameters were generated from 625 subjects. The models in this study achieved an area under the ROC curve (AUC) of 0.880 in predicting hyperglycemia, of 0.766 in predicting hypertension, and of 0.703 in predicting dyslipidemia. In addition, these models can predict with AUC>0.7 several blood test erythrocyte parameters, including hematocrit (HCT), mean corpuscular hemoglobin concentration (MCHC), and a cluster of cardiovascular disease (CVD) risk factors. Taken together, deep learning approaches are feasible for predicting hypertension, dyslipidemia, diabetes, and risks of other chronic diseases.

Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi

In the development of diabetic complications, oxidative stress caused by hyperglycemia plays an important role. Deuterium depletion has protective effect against cellular damage and tumor suppressor effect in cancer cell lines. The purpose of this study was to examine and evaluate the effect of deuterium depletion on erythrocyte oxidant and antioxidant system parameters in experimental diabetes induced by streptozotocin (STZ) in rats. On the 30th or 40th day after STZ injection, blood samples were taken and blood glucose levels were measured. Erythrocyte hemolysates were also prepared. Lipid peroxidation and glutathione levels and glutathione-S-transferase, superoxide dismutase and catalase enzyme activities of erythrocytes were determined. At the 30 or 40 day periods, deuterium depleted water (DDW) consumption had differently affected oxidant-antioxidant parameters of erythrocytes. The clear effect of deuterium depletion in drinking water from 150 ppm to 85 ppm was observed in glutathione levels and it increased erythrocyte glutathione levels in both the control and diabetic groups. In conclusion, deuterium depletion may have protective effects on diabetes induced oxidative stress. Further studies are needed to be done to determine the mechanism of action and the useful dosage and the application period of DDW.

DOSE-DIFFERENTIATED CORRELATION INTERACTIONS BETWEEN INDICES OF HEMOGLOBIN EXCHANGE IN TURKEYS UNDER THE INFLUENCE OF THE PROBIOTIC FORMULATION VETOM 1.2

The effect of the probiotic preparation Vetom 1.2 on the basis of Bacillus subtilis VKPM B-10641, Bacillus amyloliquefaciens VKPM B-10642 and Bacillus amyloliquefaciens VKPM B-10643 in different doses on the correlation interactions between the parameters of turkey hemoglobin exchange, blood erythrocytes and blood red blood cells direct and total bilirubin, total protein and serum albumin was studies. Correlations were calculated using Spearman formula, the reliability of correlations was checked by Student. The formulation was used for turkeys once a day for 30 days in doses of 12.5; 25; fifty; 75 and 100 mg / kg body weight. It was found that Vetom 1.2 in minimal doses leads to an inverse correlation between iron and total bilirubin, direct bilirubin, total protein and albumin, an increase in the concentration of the formulation leads to the disappearance of this effect with a gradual subsequent decrease in this indicator. Vetom 1.2 in doses up to 50 mg / kg leads to an inverse correlation between the concentrations of hemoglobin and direct bilirubin, total protein and serum albumin, at a dose of 75 mg / kg there is a direct correlation between these indicators, and at a dose of 100 mg / kg returns a negative correlation. The probiotic preparation Vetom 1.2 leads to a direct dependence of the concentration of bilirubins (total and direct) in serum on the concentrations of hemoglobin and red blood cells when used in most doses, except 75 mg / kg, which reduces the correlation.

Iron homeostasis in a mouse model of thalassemia intermedia is altered between adolescence and adulthood.

BackgroundIron overload is one of common complications of β-thalassemia. Systemic iron homeostasis is regulated by iron-regulatory hormone, hepcidin, which inhibits intestinal iron absorption and iron recycling by reticuloendothelial system. In addition, body iron status and requirement can be altered with age. In adolescence, iron requirement is increased due to blood volume expansion and growth spurt. Heterozygous β-globin knockout mice (Hbbth3/+; BKO) is a mouse model of thalassemia widely used to study iron homeostasis under this pathological condition. However, effects of age on iron homeostasis, particularly the expression of genes involved in hemoglobin metabolism as well as erythroid regulators in the spleen, during adolescence have not been explored in this mouse model.MethodsIron parameters as well as the mRNA expression of hepcidin and genes involved in iron transport and metabolism in wildtype (WT) and BKO mice during adolescence (6–7 weeks old) and adulthood (16–20 weeks old) were analyzed and compared by 2-way ANOVA.ResultsThe transition of adolescence to adulthood was associated with reductions in duodenal iron transporter mRNA expression and serum iron levels of both WT and BKO mice. Erythrocyte parameters in BKO mice remained abnormal in both age groups despite persistent induction of genes involved in hemoglobin metabolism in the spleen and progressively increased extramedullary erythropiesis. In BKO mice, adulthood was associated with increased liver hepcidin and ferroportin mRNA expression along with splenic erythroferrone mRNA suppression compared to adolescence.ConclusionOur results demonstrate that iron homeostasis in a mouse model of thalassemia intermedia is altered between adolescence and adulthood. The present study underscores the importance of the age of thalassemic mice in the study of molecular or pathophysiological changes under thalassemic condition.

Genetic polymorphism of calcium metabolism regulators in differential susceptibility to the effects of lead

Based on an examination of 102 employees of lead-acid batteries recycling plant, there have been determined associations of single nucleotide polymorphisms of calcium metabolism regulators genes VDR (rs10735810) and CALCR (rs187197) and blood lead levels, urine ALA, erythrocytic parameters, that indicates significance of these genetic markers as indicators of susceptibility to lead exposure.

Влияние газотрансмиттера сероводорода на микрореологические свойства эритроцитов здоровых лиц и больных сахарным диабетом второго типа

Цель исследования: изучение влияния донора газотрансмиттера (ГТ) сероводорода (NaНS) и активатора его внутриклеточного синтеза (Lцистеина) на микрореологические характеристики эритроцитов в норме и при сахарном диабете 2-го типа (СД2). Материалы и методы. Эритроциты 16 здоровых лиц и 16 больных СД2 инкубировали с донором сероводорода гидросульфидом натрия (NaHS) в концентрации 10, 50 и 100 мкмоль и стимулятором его эндогенного синтеза Lцистеином в концентрации 10, 50 и 100 мкмоль. Блокировали АТФзависимые калиевые каналы глибенкламидом (50 мкмоль). Ингибировали растворимую гуанилатциклазу с помощью 0,5 мкмоль ODQ (1H-1,2,4-oxadiazolo4,3-a quinoxalin-l-one). Регистрировали деформируемость (ИУЭ) и агрегацию эритроцитов. Результаты. Под влиянием NaHS наблюдали дозозависимые положительные изменения микрореологии эритроцитов. Микрореологические ответы эритроцитов на NaHS были более выраженными у здоровых лиц, чем у больных СД2. Сходную картину наблюдали и при воздействии на эритроциты Lцистеином. Прединкубация с глибенкламидом не устраняла положительного влияния донора ГТ на микрореологию эритроцитов, тогда как ингибирование растворимой гуанилатциклазы с помощью ODQ полностью устраняло положительные изменения ИУЭ под действием ГТ и существенно уменьшало его влияние на агрегацию клеток. Заключение. Таким образом, сероводород как газотрансмиттер улучшает микрореологию эритроцитов. При одинаковой концентрации донора сероводорода (NaHS) и стимулятора его синтеза (Lцистеина) более выраженные сдвиги микрореологических параметров эритроцитов наблюдаются у здоровых лиц по сравнению с больными СД2. Aim: to study the effect of donor gasotransmitter (GT) hydrogen sulphide (NaHS) and the activator of its intracellular synthesis (Lcysteine) on the red blood cells (RBCs) microrheological characteristics in normal and diabetes mellitus type 2 (DM2). Materials and methods. RBCs of 16 healthy individuals and 16 patients with DM2 were incubated with hydrogen sulfide donor sodium hydrosulfide (NaHS) in concentrations of 10, 50 and 100 mol and a stimulator of its endogenous synthesis Lcysteine (10, 50 and 100 mol). ATPdependent potassium channels were blocked by glibenclamide (50 mol). Soluble guanylate cyclase was inhibited using 0.5 mol ODQ (1H-1,2,4-oxadiazolo4,3-a quinoxalin-l-one). Deformability (RBCD) and RBCs aggregation were recorded. Results. Under the influence of NaHS, dose-dependent positive changes in RBCs microrheology were observed. Cellular microrheological responses to GT donor were more pronounced in healthy individuals than in patients with DM2. A similar pattern was observed when RBCs were exposed to Lcysteine. Preincubation with glibenclamide did not eliminate the positive effect of GT donor on RBCs microrheology. Whereas inhibition of soluble guanylate cyclase with the help of ODQ completely eliminated positive changes in RBCD under the action of GT and significantly reduced its effect on aggregation. Conclusion. Thus, hydrogen sulfide as a gasotransmitter improves RBCs microrheology. With the same concentration of hydrogen sulphide donor (NaHS) and its synthesis stimulator (Lcysteine), more pronounced shifts in the microrheological parameters of erythrocytes are observed in healthy individuals as compared with DM2 patients.

Hematological Characteristics of Hb Constant Spring (HBA2: c.427T>C) Carriers in Mainland China

Hb Constant Spring (Hb CS) (HBA2: c.427T>C) is a common α-globin variant causing α-thalassemia (α-thal) phenotypes in mainland China. In this study, we evaluated the efficiency of erythrocyte parameters and capillary electrophoresis (CE) in the determination of Hb CS in blood samples from Hb CS carriers. Based on molecular diagnosis, there were 462 patients carrying Hb CS: 411 Hb CS heterozygotes, seven carried Hb H-Hb CS disease, 18 compound heterozygotes for Hb CS/α+-thal, and 26 double heterozygotes for Hb CS and β-thalassemia (β-thal). Forty-three cases had no Hb CS peak visible on CE, including all 26 cases of double heterozygotes for Hb CS and β-thal, and 17 cases of heterozygotes carrying only Hb CS. Hb CS heterozygotes, those without a Hb CS peak, presented with lower hemoglobin (Hb), mean corpuscular volume (MCV) and mean corpuscular Hb (MCH) values than those with a Hb CS peak. The MCV <80.0 fL yielded a detection rate of 87.8% for screening individuals carrying Hb CS. Therefore, we emphasize that if one partner of a couple has tested positive for α0-thal, the other should be subjected to detailed screening for this nondeletional allele using molecular analysis, regardless of his/her red cell indices and electrophoretic chromatogram.

Ammonium Salts Promote Functional Adaptation of Rat Erythrocytes on the Model of Forced Swimming

Ammonium, an end-product of catabolism, in low doses can promote adaptation of metabolic pathways in erythrocytes under conditions of extreme physical exercise. We compared the effects of two ammonium salts, ammonium chloride and ammonium carbonate, in two doses on biochemical parameters of rat erythrocytes 1 day after extreme physical exercise in a 4-week cycle of forced swimming. Of 16 analyzed parameters, the maximum number of significant shifts from the control was revealed in the groups of rats receiving ammonium chloride in doses of 20 and 10 mg/kg, and the minimal number of differences was found in groups treated with ammonium carbonate in the same doses. The comparison of the levels of reduced glutathione and 2.3-bisphosphoglicerate and activities of 5'-nucleotidase and Ca2+- and Na/K-ATPases attested to more rigorous control of the mechanism of oxygen delivery to tissues by erythrocytes after administration of ammonium chloride in a dose of 20 mg/kg.

Repair of Erythrocyte Membranes by the Lipid Fraction from Brown Seaweed Sargassum Pallidum after Experimental Ccl4-Induced Toxic Hepatitis

The influence of the lipid fraction from the aqueous EtOH extract of brown seaweed Sargassum pallidum thallus and the commercial reference drug Essentiale® on the physiological characteristics of rat erythrocytes and the lipid composition of erythrocyte membranes after CCl4-induced toxic injury was studied. CCl4 intoxication (50% solution in olive oil, s.c., 2 mL/kg for 4 d) was associated with changes of all studied physiological and biochemical erythrocyte parameters that did not return to normal after withdrawing the toxicant. Intragastric administration to rats through a probe of the lipid fraction from S. pallidum extract at a dose of 80 mg of total lipids per kg of mass for 7 d after CCl4 intoxication gave membrane protection that manifested as a restoration of the characteristic erythrocyte dimensions (average volume and diameter), extension of the threshold to their hemolysis stability, and retention of the lipid constituent ratio in the membrane. The lipid fraction from brown seaweed S. pallidum was comparable to Essentiale® for restoring erythrocyte physiological characteristics and the lipid composition of their membranes after CCl4-induced damage. The pronounced protective effect of the S. pallidum lipid fraction for repairing erythrocyte membranes was evidently determined by the combined action of the marine lipids in it.

Hemolytic specimens in complete blood cell count: Red cell parameters could be revised by plasma free hemoglobin.

IntroductionHemolysis is the main cause of unqualified clinical samples. In this study, we established a method for detecting and evaluating hemolysis in whole blood test. We used a mathematical formula for correcting the influence of hemolysis on complete blood cell count (CBC) so as to avoid re‐venipuncture and obtain more accurate parameters of red blood cell detection, reduce the burden of patients, and improve the efficiency of diagnosis and treatment.MethodsHemolytic samples were selected and then corrected using the new formula. Plasma free hemoglobin (fHB) was used as the criterion to determine the degree of hemolysis; the uncertainty of measurement is acceptable as the limit value of deviation between the measured value and the revised value. Hemolysis simulation analysis in vitro and continuous monitoring of clinical patients were used to verify the correction effect.ResultsA total of 83 clinical samples with hemolysis were collected and analyzed; fHB 1.4 g/L was selected as the unacceptable value for clinical hemolysis detection. In hemolytic samples, the red blood cell parameters corrected by formula are significantly different from those uncorrected and had a good consistency with those before hemolysis.ConclusionThe results show that the hemolysis phenomenon of CBC has a significant impact on routine blood testing. By using the new formula, the influence of hemolysis on erythrocyte and related parameters can be quickly and easily corrected, thus avoiding venipuncture again for re‐examination, reducing diagnostic errors, and saving medical resources.

P068 Erythrocyte and reticulocyte parameters in children with inflammatory bowel diseases

Abstract Background Anaemia is a frequent extraintestinal manifestation of inflammatory bowel diseases (IBD) and its severity leads to a deterioration in patient’s condition. Timely diagnosis and pathogenetic therapy are important for the effective treatment. The aim of this study was to evaluate the current cell indexes obtained from automatic blood analyser to identify the pathogenetic features of anaemia in children with IBD. Methods A total of 1400 blood samples were analysed in 420 children with IBD (177—Crohn’s disease (CD) and 243—ulcerative colitis (UC)) followed up in our centre in 2018–2019. A blood test was performed by flow hemocytometry on an automatic blood analyser Sysmex XN 1000. The evaluated parameters were: MCV, MCH, MCHC, RET, IFR and RET-He. Anaemia was established with haemoglobin level &amp;lt;110 g/l. Clinical activity was evaluated according PUCAI (UC) and PCDAI (CD) scores. Results Anaemia was detected in 31.7% of patients with UC and in 26.8% of patients with CD. Decreasing of Hb level was observed in both diseases with an increasing in the clinical activity. The average Hb levels in CD in remission, moderate and severe activity were 132.15 ± 17.07, 120.51 ± 18.28 and 105.06 ± 12.46 g/l, respectively; in children with UC with remission, minimal, moderate and severe activity 124.86 ± 16.01; 114.53 ± 15.56; 109.71 ± 20.34 and 96.09 ± 16.52 g/l, respectively. In patients with CD, anaemia was predominantly hypochromic: the average values of MCV, MCH and RET-He indicates a leading role the bioavailable iron deficiency and corresponded to those in CD remission: 82.8 ± 7.0 fl, 27.35 ± 3.02 pg and 31.28 ± 3.82 pg; in low/moderate CD activity: 78.93 ± 7.49 fl, 25.29 ± 3.51 pg, 28.62 ± 5.1 pg; in high CD activity: 74.7 ± 6.74 fl, 22.99 ± 2.69 pg, 27.14 ± 4.99 pg, respectively. RET count in CD was moderate and equal 58–68 cell/ml. With an increase in the UC process activity, hypochromia was not enhanced: MCV in the range 79–82 fl, MCH—25–26 pg. RET-He in remission of UC were 30.22 ± 3.95 pg; in minimal activity—28.93 ± 4.96 pg; in moderate activity—27.99 ± 4.62 pg and only with high activity of UC there was a decreasement to 25.21 ± 4.99 pg. In UC the level of RET and IFR increases with increasing severity of the disease: 57.75 ± 17.48‰, 63.31 ± 31.12‰, 77.29 ± 41.81‰, 111.29 ± 78,9‰ and IFR 11.62 ± 5.235%, 15.69 ± 8.065%, 18.97 ± 8.385%, 26.97 ± 9.785%, respectively. This suggests that anaemia in UC is mainly associated with blood loss. Conclusion Anaemia in different forms of IBD in children has different pathogenetic mechanisms. Evaluation of current parameters of red blood cells by modern automatic blood count analyser is cheap and informative for the differential diagnosis of anaemic syndrome in children with IBD.

Effects of Pausinystalia Yohimbe Methanol Root Extract on Some Cardiovascular and Hematological Variables in Wistar Rats

Plants have been a major source of medicine and have been largely considered to alter the range of blood pressure and parameters. The present study was undertaken to investigate the effect of Pausinystalia yohimbe on blood pressure and haematological factors in normotensive male wistar rats. The rats were randomly divided into four groups of nine rats each. The control group received 1 mL/kg distilled water while the three treatment groups received 25 mg/kg, 50 mg/kg and 100 mg/kg of the extract respectively, and the blood pressure was measured three hours thereafter. After the study period, blood samples were taken from experimental rats, and haematological factors were determined using digital cell counter. Administration of the extract, caused a dose-dependent fall in blood pressure and heart rate, and appeared to increase leucocyte (WBC and lymphocytes) and erythrocyte parameters (RBC, HCT and Hgb) when compared with the control group. While thrombocyte parameters (PLT and Pct) were decreased. The findings demonstrated that acute hypotensive and antitarchycardiac effect of the extract may be due to direct effect of the metabolites on cardiac-vascular smooth muscle, and that Pausinystalia yohimbe does not cause any significant undesirable alterations in haematological factors in wistar rats. It also enhances white blood cell concentration and lymphocytes which probably stimulate the immune defence mechanism.

Исследование и оценка гематологических показателей крови и морфометрических параметров эритроцитов крыс при воздействии наночастиц оксида кальция

Исследование и оценка гематологических показателей крови и морфометрических параметров эритроцитов крыс при воздействии наночастиц оксида кальция

Ameliorative Effects of Aqueous Extract of Citrullus Lanatus Fruit on Phenylhydrazine Induced Anemia In Male Wistar Rats

The possible ameliorative effects of aqueous extract of Citrullus lanatus juice(CLJ)on hematological parameters, serum erythrocytic osmotic fragility and lipid profile parameters were investigated following phenylhydrazine(PHZ)administration using wistar rats. Thirty male wistar rats were randomly distributed into 6 groups of 5 rats each. Group A were untreated controls; Group B received only phenylhydrazine negative controls; Group C received phenylhydrazine+1 ml/kg/day of Vitamin B12 syrup from day 2 to day14; Groups D, E and F rats were anemia induced with phenylhydrazine and treated with different doses (100, 300 and 600mg/kg) of CLJ extracts respectively and compared with Group A rats. The injection of PHZ to rats caused hemolytic anemia characterized by reducing hematological parameters.The oral administration of CLJ in the doses of 300 mg/kg and 600 mg/kg significantly increased Haemoglobin level. Also, the results demonstrated that the use of CLJ can as well maintain Red blood cell membrane integrity.The lymphocytes values was significantly elevated in dose 300mg/kg and 600mg/kg of the extract suggesting an increasing dose dependence effects of the extract.It was observed that the values of serum lipid profile, total cholesterol and Low density lipoprotein in anemic rats and treated rats with 100 mg/kg and 300mg/kg doses of the CLJ extract were significantly higher than the control Group A,which is consistent with the findings of some studies. These results suggest a possible ameliorative potential of Citrullus lanatus juice on hematological parameters and immunostimulating with indices of hematology following phenylhydrazine administration. This result shows that CLJ extract can be used to treat anemia.

Dielectric properties of the human red blood cell

The dielectric properties of the erythrocyte were studied. Experimental methods and results of measuring the dielectric properties of individual human erythrocyte are presented. The method is theoretically justified. It is established that values of the complex permittivity of the erythrocyte, capacitance, tangent of dielectric losses remains almost constant despite significant changes in conductivity, the content of NaCl of the cell suspension. These values reflect the stability of the studied parameters of erythrocytes under conditions of changes within a wide range of the environment in which the cells are located. Complex cell permittivity and cell dielectric loss tangent are promising parameters for objective diagnostics of human diseases. The introduction of measurements of cell permittivity and cell dielectric loss tangent into medical practice will probably allow us to characterize the process of individual cell self-regulation in more detail.

PROGNOSIS OF ACUTE LEUKEMIA DEPENDING ON THE IRON METABOLISM PARAMETERS IN CHILDREN AFTER CHORNOBYL NUCLEAR POWER PLANT ACCIDENT.

To determine the influence of iron metabolism on the prognosis of acute lymphoblastic (ALL) and (AML)myeloblastic leukemia at the different phases of chemotherapy in children after Chоrnobyl accident. 333 children (295 - ALL, 38 - AML) were examined at the stages of chemotherapy. Thecomparison group included 93 children without leukemia. Acute leukemia variants, patients survival, relapses, thenature of disease (live child or died), iron methabolism (morphometric parameters of erythrocytes, SI, SF, STf, TS),manifestations of dyserythropoiesis, bone marrow sideroblast and patients radiation dose were taken into account. In 295 patients with ALL the following variants of leukemia were established: pro-B-ALL in 23, «common»type of ALL in 224, pre-B-ALL in 29, T-ALL in 19. Thirty eight patients were diagnosed with AML (11 - M1, 19 - M2,8 - M4). Doses of radiation in patients with AL were (2.78 ± 0.10) mSv and they did not correlate with clinical andhematological parameters, disease variant. Relapse rates and shorter survival were in patients with T-ALL, pro-B-ALLand AML with SF levels > 500 ng/ml (p < 0.05). The amount of children with normochromic-normocytic anemias andmanifestations of dysplasia of erythroid lineage elements was greater in the AML than in ALL. SF content in patientswas elevated during chemotherapy and was lower than the initial one only in the remission period. Transferrin wasreliably overloaded with iron: TS (70.2 ± 2.3) % compared with the control group (32.7 ± 2.1) %. Correlationbetween TS and survival of patients was detected (rs = -0.45). Direct correlation between the number of iron granules in erythrocariocytes and SF level (rs = 0.43) was established, indicating the phenomena of ineffective erythropoiesis. The negative influence of iron excess in the patients body on the hemopoiesis function, manifestations of ineffective erythropoiesis and the course of acute leukemia in children have been established. Changes inferrokinetic processes in children can be the basis of leukemоgenesis development.

POSSIBILITIES OF THE METHOD OF DIELECTROPHORESIS OF ERYTHROCYTES IN DISTINCTION OF PATIENTS WITH FATTY LIVER DISEASE OF ALCOHOLIC AND NON-ALCOHOLIC GENESIS

The aim of the work is to study the possibilities of distinguishing between men with fatty disease of alcoholic and non-alcoholic origin using the viscoelastic parameters of erythrocytes - deformation amplitude, summerized indicators of viscosity and rigidity (baseline and after exposure to ethanol in vitro) obtained using the method of erythrocyte dielectrophoresis; to determine associations of the amplitude of deformation, summerized viscosity and rigidity of erythrocytes with blood lipid levels. The study involved 54 men (44,62±1.52 years) with fatty liver disease according to ultrasound of the abdominal organs, the severity of fibrosis corresponded to 0-1 (FibroScan® 502 Echosens, France). All patients underwent a study of the viscoelastic parameters of erythrocytes - the amplitude of deformation, summerized indicators of viscosity and rigidity by the dielectrophoresis method: the baseline level of indicators was determined, as well as their values after exposure of red blood cells with 10 μl of 0,0 2% ethanol solution in vitro for 300 s. The dynamics of changes in the viscoelastic parameters of erythrocytes during the experiment with alcohol in patients with fatty liver disease of unknown origin made it possible to highlight two groups with diametrically opposite trends in erythrocyte indices. The group with a decrease in the amplitude of erythrocyte deformation against the background of an increase in summerized viscosity and rigidity ( n = 26) consisted mainly of patients with metabolic syndrome who do not drink or occasionally consume alcohol in low doses (less than 20 g in terms of pure ethanol). Group with increased erythrocyte deformability and decrease in summerized viscosity and rigidity ( n = 28) includes systematic alcohol consumers who were in a state of abstinence. Ethanol exposure, important energy substrate of this group, built into metabolic processes, led to an increase in the amplitude of cell deformation. Correlations of the viscoelastic parameters of erythrocytes with the style of alcohol consumption, components of the metabolic syndrome, lipid profile indicators, and liver tests were established. Direct associations of the amplitude of erythrocyte deformation with the level of HDL cholesterol and inverse - with the values of total cholesterol, LDL cholesterol, triglycerides were identified. Summarized viscosity and rigidity correlated with the levels of these lipid indicators inversely compared with the amplitude of deformation. The diagnostic accuracy of distinguishing between NAFLD and AFLD using models, including the viscoelastic parameters of erythrocytes and their changes after exposure to ethanol, using Random Forest and SVM methods reached 99 %, sensitivity of 98 % and specificity of 99 %.