Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi

Abstract

In the development of diabetic complications, oxidative stress caused by hyperglycemia plays an important role. Deuterium depletion has protective effect against cellular damage and tumor suppressor effect in cancer cell lines. The purpose of this study was to examine and evaluate the effect of deuterium depletion on erythrocyte oxidant and antioxidant system parameters in experimental diabetes induced by streptozotocin (STZ) in rats. On the 30th or 40th day after STZ injection, blood samples were taken and blood glucose levels were measured. Erythrocyte hemolysates were also prepared. Lipid peroxidation and glutathione levels and glutathione-S-transferase, superoxide dismutase and catalase enzyme activities of erythrocytes were determined. At the 30 or 40 day periods, deuterium depleted water (DDW) consumption had differently affected oxidant-antioxidant parameters of erythrocytes. The clear effect of deuterium depletion in drinking water from 150 ppm to 85 ppm was observed in glutathione levels and it increased erythrocyte glutathione levels in both the control and diabetic groups. In conclusion, deuterium depletion may have protective effects on diabetes induced oxidative stress. Further studies are needed to be done to determine the mechanism of action and the useful dosage and the application period of DDW.