Pantoea Spp Research Articles

Pantoea spp. Application to Increase the Growth and Yield of Rice ‘Pathum Thani 1’

Pantoea spp. Application to Increase the Growth and Yield of Rice ‘Pathum Thani 1’

Biological control of Ralstonia solanacearum (Smith), the causal pathogen of bacterial wilt disease by using Pantoea spp

BackgroundBacterial wilt of tomato (BWP) caused by Ralstonia solanacearum (Smith) is a very important disease. Biological control of this disease is a very important tool to protect the plant and environment from pollution of chemical control.ResultsTwenty isolates of genus, Pantoea were isolated from healthy tomato root. Out of 20 isolates, 2 strains, PHYTPO1 and PHYTPO2, showed highly antagonistic property to control the growth of R. solanacearum in vitro conditions. They were identified as P. agglomerans by using 16S rRNA nucleotide sequence analysis. The 2 isolates were selected to study their effect (as cell suspension or culture filtrate) on the bacterial wilt under greenhouse conditions. PHYTPO1 inhibited maximum growth reduction of R. solanacearum and formed 2.5 cm2 of inhibition zone, followed by 1.2 cm2 in PHYTOPO2 under in vitro conditions. Treating with both isolates of P. agglomerans was significantly reduced disease severity of tomato wilt disease. The disease severity was reduced to 74.1 when treated as cell suspension, while when treated as culture filtrate, it reduced the disease severity up to 69.4 than infected control.ConclusionThe strains of Pantoea can be used as an ecofriendly method to control of the most economic pathogen of tomato under greenhouse conditions. Further study is needed to find an appropriate formulation and approving application of these bacteria under field conditions.

Biochemical characteristics and antibiotic resistance of bacterial isolate from Ctenocephalides felis

Cats are the most domesticated animals kept by humans in the world. Cat fleas are ectoparasites that have the potential to transmit disease caused by microbes in humans. Biochemical identification research and antibiotic resistance tests have been carried out against bacterial isolates from cat fleas. Cat fleas were isolated from cats in Manado City, North Sulawesi, Indonesia. Isolation of bacteria was conducted using the scratch method on nutrient agar media. The pure bacterial culture is then used for biochemical analysis and antibiotic resistance testing. Biochemical analysis and resistance tests were performed using the Vitec 2 Compact instrument with a standardized automatic analysis model. The results obtained three species of bacteria from cat fleas based on biochemical identification, namely S. equorum, C. freundii, and Pantoea spp. Antibiotic resistance test on S. equorum showed that of the 60 types of antibiotics used, 55 were sensitive and 7 were resistant. Furthermore, C. freundii, of the 18 types of drugs, 7 were sensitive and 11 were resistant. Meanwhile, in Pantoea spp., Sensitive and resistant drugs were not found. However, the results of this study prove that bacteria from cat fleas have the potential to infect humans with relatively high antibiotic resistance.

ПРОРАСТАНИЕ СЕМЯН ЗЛАКОВ ПОД ВЛИЯНИЕМ КОМПОЗИЦИЙ АЗОТФИКСИРУЮЩИХ И ФОСФАТМОБИЛИЗУЮЩИХ БАКТЕРИЙ ИЗ ПОЧВ, ВОЗДЕЛЫВАЕМЫХ В УСЛОВИЯХ ДАЛЬНЕГО ВОСТОКА

Mineral fertilizers which can significantly increase crop productivity have an adverse effect on the soil and the environment as a whole when used for a long time. Microorganisms, as an alternative to mineral fertilizers, stimulate plant growth and development due to their ability to fix nitrogen, produce siderophores, phytohormones and enzymes, dissolve inaccessible elements of mineral nutrition, suppress plant pathogens, and increase consumption of water and nutrients. However, the effectiveness of such preparations highly depends on factors of a new environment. We believe that bacteria from soils that have long been exposed to various agricultural practices may be good plant stimulants. In the presented study, for the first time, we have isolated local active strains of nitrogen-fixing and phosphate-mobilizing bacteria from soils subjected to 74-year stationary intensive farming in the conditions of the Russian Far East and revealed isolates and their combinations which stimulate wheat and barley seed germination and seedling growth. The aim of the work was to study the plant-stimulating properties of nitrogen-fixing and phosphate-mobilizing bacteria from soils that have been actively exposed to mineral fertilizers for a long time. Bacteria were isolated from soils sampled in October 2015 (the experimental field 8, Federal Research Center for Agrobiotechnology FEB RAS, Ussuriysk, Primorsky Territory, Russia). The seeds of wheat Triticum aestivum L. cultivar Primorskaya 50, and barley Hordeum vurlage L. cultivar Tikhookeanskii (collection of the Federal Research Center for Agrobiotechnology FEB RAS) were treated. Of 68 bacterial isolates with different cultural and morphological properties, three isolates, the Acinetobacter spp. N1, Azotobacter spp. N2, and Clostridium spp. N3 were nitrogen fixers, and four isolates, the Serratia spp. P6, Bacillus spp. P7), Arthrobacter spp. P8, and Pantoea spp. P19 were phosphate-mobilizing bacteria. Tests with the monocultures of nitrogen-fixing and phosphate-mobilizing isolates and their different binary compositions showed a 13-51 % increase (p ≤ 0.05) in wheat seed germination energy and 15-54 % increase (p ≤ 0.05) in barley seed germination energy compared to the untreated control. Laboratory germination of wheat seeds increased by 2-32 %, barley seeds by 7-30 % compared to untreated control. The barley seedlings were 1.8 times longer, and the roots were 2.7 times longer. Th binary combination N2P19, P6P19, and P8P19 caused the highest height of seedlings (120-140 mm, p ≤ 0.05), and with P6P7, N2P19, and P6P19 the roots were the longest (120-130 mm, p ≤ 0.05). These results allow us to conclude that short-term soaking seeds in the suspensions of the tested nitrogen-fixing and phosphate-mobilizing isolates improves seed germination energy and laboratory germination, and increases shoot and root length. Binary bacterial compositions have a greater effect on seed germination than monocultures. The strains N1 (Acinetobacter spp.), N2 (Azotobacter spp.), and P19 (Pantoea spp.) are the best stimulants. Species-specific differences in plant response to the treatment is probably due to lack of genetic, biochemical and physiological complementarities between specific plant species and the bacteria.

EFFECTS OF NITROGEN-FIXING AND PHOSPHATE-SOLUBILIZING MICROORGANISMS FROM THE FAR EAST AGRICULTURAL SOILS ON THE CEREAL SEED GERMINATION

Mineral fertilizers which can significantly increase crop productivity have an adverse effect on the soil and the environment as a whole when used for a long time. Microorganisms, as an alternative to mineral fertilizers, stimulate plant growth and development due to their ability to fix nitrogen, produce siderophores, phytohormones and enzymes, dissolve inaccessible elements of mineral nutrition, suppress plant pathogens, and increase consumption of water and nutrients. However, the effectiveness of such preparations highly depends on factors of a new environment. We believe that bacteria from soils that have long been exposed to various agricultural practices may be good plant stimulants. In the presented study, for the first time, we have isolated local active strains of nitrogen-fixing and phosphate-mobilizing bacteria from soils subjected to 74-year stationary intensive farming in the conditions of the Russian Far East and revealed isolates and their combinations which stimulate wheat and barley seed germination and seedling growth. The aim of the work was to study the plant-stimulating properties of nitrogen-fixing and phosphate-mobilizing bacteria from soils that have been actively exposed to mineral fertilizers for a long time. Bacteria were isolated from soils sampled in October 2015 (the experimental field 8, Federal Research Center for Agrobiotechnology FEB RAS, Ussuriysk, Primorsky Territory, Russia). The seeds of wheat Triticum aestivum L. cultivar Primorskaya 50, and barley Hordeum vurlage L. cultivar Tikhookeanskii (collection of the Federal Research Center for Agrobiotechnology FEB RAS) were treated. Of 68 bacterial isolates with different cultural and morphological properties, three isolates, the Acinetobacter spp. N1, Azotobacter spp. N2, and Clostridium spp. N3 were nitrogen fixers, and four isolates, the Serratia spp. P6, Bacillus spp. P7), Arthrobacter spp. P8, and Pantoea spp. P19 were phosphate-mobilizing bacteria. Tests with the monocultures of nitrogen-fixing and phosphate-mobilizing isolates and their different binary compositions showed a 13-51 % increase (p ≤ 0.05) in wheat seed germination energy and 15-54 % increase (p ≤ 0.05) in barley seed germination energy compared to the untreated control. Laboratory germination of wheat seeds increased by 2-32 %, barley seeds by 7-30 % compared to untreated control. The barley seedlings were 1.8 times longer, and the roots were 2.7 times longer. Th binary combination N2P19, P6P19, and P8P19 caused the highest height of seedlings (120-140 mm, p ≤ 0.05), and with P6P7, N2P19, and P6P19 the roots were the longest (120-130 mm, p ≤ 0.05). These results allow us to conclude that short-term soaking seeds in the suspensions of the tested nitrogen-fixing and phosphate-mobilizing isolates improves seed germination energy and laboratory germination, and increases shoot and root length. Binary bacterial compositions have a greater effect on seed germination than monocultures. The strains N1 (Acinetobacter spp.), N2 (Azotobacter spp.), and P19 (Pantoea spp.) are the best stimulants. Species-specific differences in plant response to the treatment is probably due to lack of genetic, biochemical and physiological complementarities between specific plant species and the bacteria.

Determination some virulence genes (avrxacE2, avrxacEl, hrpG, acrAB) in Pantoea spp. isolated from hospitals of Al-Diwaniyah city, Iraq

Pantoea spp. is one of the opportunistic pathogens, which causes the spread of hospital infection due to its transformation from bacteria to plant organisms to human pathogenic bacteria. A total of 623 samples were collected and distributed as 483 samples of different clinical cases (77.52%) and 140 environmental samples (22.47%) from Diwaniyah city hospitals. The results showed that 24 isolates of pantoea spp.s had been confirmed using API-20E Polymerase chain reaction technology. The current study reported the presence of two types of pathogens for the Pantoea spp.: the first is Pantoea agglomerans and Pantoea calida in hospitals in the city of Diwaniyah. Pantoea agglomerans was occurring in the studied isolates at a higher rate (2.27%) than Pantoea calida. All isolates of Pantoea spp were tested for the prevalence of virulence genes (avrxacE2, avrxacEl, hrpG, acrAB) using the polymerase chain reaction (PCR) technique. The highest incidence of hrpG was recorded as 79.16%, followed by avraxacE2 gene were 13 (54.16%). The current study did not record any presence of the virulence genes (avrxacE1 acrAB) among the isolates of Pantoea spp.

Assessment of Serum IL8 and TLR-4 Concentrations Associated with Pantoea spp. Persistence in Recurrent UTI Patients

Assessment of Serum IL8 and TLR-4 Concentrations Associated with Pantoea spp. Persistence in Recurrent UTI Patients

Epidemiology of bacterial Septicemia among children under five in Mbita Subcounty, South Nyanza, Kenya

Background: Septicaemia is a major cause of mortality and morbidity, especially in sub-Saharan Africa leading to complications marked by bodily inflammation referred as sepsis. This is a systemic disease associated with presence of pathogenic microorganisms (viral, parasitic and bacterial) or their toxins in the blood. Bacterial septicaemia is the most fatal and prevalent in hospitalised cases. Globally, 76% of children under five years die due to septicaemia. In East Africa a mortality rate of 40% have been reported. In Kenya, South Nyanza regions have reported higher morbidity and mortality cases among children. We hypothesis that apart from immunosuppressive diseases, septicaemia could contribute significantly to this prevalence in the region. Methods: Blood samples were obtained from 248 children whose guardian consented and a detailed sociodemographic questionnaire was administered. Bacterial isolation and characterization were done using the automated BACTEC 9240 system. Results: The mean age of the participants was 27.9 (SD ±20.7) months. The majority (30.6%) were aged between 1 to 12 months, 50.8% were males, 58.9% had body temperatures above 37.6 O C while only 8.1% were HIV seropositive. The mean white blood cells (WBC) of the participants were 17720.9 (SD 8929.1) cells/ml with 5.2% had leucopenia. A total of 84 of the 248 (33.9%) of the children had septicaemia with the majority (28.6%) caused by Staphylococcus epidermidis followed by Staphylococcus aureus and Escherichia coli each at 13.1%. Bacteria that were reported singly included Salmonella Paratyphi B, Citrobacter freundii , Gemella morbillorum , Klebsiella pneumoniae , Lactococcus lactis cremoris , Pantoea spp, and Pseudomonas putida . In multivariate regression analysis, female gender (OR 0.6; 95% confidence interval (CI) 0.4 to 0.9), co-infection with malaria (OR 2.7; 95% CI 1.1 to 6.7) and gastrointestinal disorders (OR 2.9, 95% CI 1.3 – 7.3) were independently associated with bacterial septicemia infection. Conclusion: Significantly higher proportion of the children in this region are infected with septicaemia. Majority of the cases were caused by Gram positive bacteria. Age and other c-infection contribute significantly to septicaemia infection in this region. Rapid testing and etiological characterisation of children with suspected symptoms of septicaemia is key in this region in order to institute appropriate treatment and management. Keywords : bacterial Septicemia, Epidemiology, Children under five, South Nyanza, Kenya DOI: 10.7176/JNSR/10-10-06 Publication date: May 31 st 2020

Antimicrobial Resistance Patterns of bacterial Septicaemia infecting infants in Mbita Subcounty, Western region of Kenya

Background: Gram positive bacteria such Escherichia coli, Group B Streptococcus coagulase-negative staphylococci, Staphylococcus aureus , and Gram-negative bacteria such as Klebsiella and Pseudomonas species are listed as some of the bacteria etiologies for pediatric septicemia. These bacteria are rapidly becoming multi drug resistant to penicillin (or aminopenicillin), gentamicin, the pragmatic antibiotic treatment regimens. Further, the ever-increasing burden of bacteria septicemia infection due to extended-spectrum β-lactamase (ESBL) producing Gram negative bacteria cumulatively presents a major health concern in the management and treatment of bacterial septicemia. In this study we present data on the prevalence and type of antimicrobial resistant patterns among children with bacterial septicemia in Mbita Sub county Hospital, Western region of Kenya. Methods: Blood samples were obtained from 248 children whose parents/guardian consented. The bacterial isolation and characterization were done using the automated BACTEC 9240 system, conventional culture using morphology, Gram stain and biochemical identification. Further identification and resistant gene detection were determined using Polymerase Chain Reaction (PCR). Descriptive statistics were used to present data. Results: Eighty-four (33.9%) patients had septicemia where Staphylococcus epidermidis (28.6%), S. aureus (13.1%), Escherichia coli (13.1%) and single Salmonella Paratyphi B, Citrobacter freundii , Gemella morbillorum , Klebsiella pneumoniae , Lactococcus lactis cremoris , Pantoea spp, and Pseudomonas putida were implicated. The majority of gram-negative bacteria were resistant to penicillin (Ampicillins) 100%, 96.1% to tetracyclin, 84.6% to sulphonamides (Trimethoprim/sulfamethoxazole), 73.1% Aminoglycosides (Gentamicin) 73.1% and 19.2% to Quinolone (Ciprofloxacin). For gram positive bacteria majority 96.7% were resistant to sulphonamides (Trimethoprim/sulfamethoxazole) followed by tetracycline 76.7%, penicillin (Oxacilline) 73.3% and least resistant to Quinolone (Ciprofloxacin) 30%. Various antimicrobial resistant genes mecA, SulII, blaTEM, TetA aac (3) were identified. Conclusion. In this geographically defined region of Kenya, of the 33.9% children with septicemia, gram positive bacteria were the leading cause septicemia. High level resistance due to various resistant genes were seen all type of antibiotics by both Gram positive and negative bacteria. Rapid antibiotic resistant testing is encouraged for appropriate treatment and management of septicemia infection. Keywords : bacterial Septicemia, Epidemiology, Children under five, South Nyanza, Kenya DOI: 10.7176/JNSR/10-10-07 Publication date: May 31 st 2020

Detection Antimicrobial Suscep Tibility ?atterns of Bacterial Species Isolated from Burns and Wounds Infections in Basrah Hospitals

Burn and wound infe?tion is ?ne of the m?st frequent s?ri?us sicknesses c?used by pathogen, chiefly b? b?th gr?m p?sitive ?nd gr?m neg?tive b?cteri?.This study was carried out to identify 25 bacteria isolated from burns and wounds surgical belong to the species : (Staphylococcus aureus,Staphylococcus lentus ,Staphylococcus intermedius,Staphylococcus lugdunensis,Pseudomonas aeruginosa ,Pseudomonas oryzihabitans,Pantoea spp., E.coli ,Rhizobium radiobacter,Ochrobactrum anthropic,Burkholdria cepacia,Sphingomonas pauci- mobilis,Klebsiella pneumoniae,Aeromonas sobria.. Staphylococcus 9(36%) was the most dominant organism,followed by Pseudomonas 6(24%).Antibacterial activity of some antibiotics was investigated against bacterial isolates . the data demonstrate the most commoly isolated from burns and wounds surgical were Staphylococcus followed by Pseudomonas .All b?ct?rial is?l?tes revealed high r?sist?nce t? antibiotics were us?d, Whereas Staphylococcus aureus revealed resistance for three antibiotic: Amikacin ,Nitazo- xanide,Neomycin . Pseudomonas aeruginosa revealed resistance for four antibiotic: Neomycin,Amoxicillin/Clavulanic acid, Cepha- lothin ,Cefotaxime. Pantoea spp revealed resistance for five antibiotic: Neomycin,Trimethoprime,Amoxicillin/Clavulanic acid, Cephalothin ,Cefotaxime . E.coli revealed resistance for four antibiotic: Neomycin ,Trimethoprime,Amikacin,Cephalothin.

Hydrocarbon-degrading genes in root endophytic communities on oil sands reclamation covers

In response to environmental regulations, the Canadian oil sands industry aims to reclaim all disturbed areas to equivalent land capability prior to mining operations. However, tailing sands used in reclamation contain residual hydrocarbons and plants growing in these areas may rely on hydrocarbon-degrading endophytic bacteria to survive. This study assessed the hydrocarbon-degrading potential (genes: CYP153, alkB and nah) of culturable and unculturable endophytic bacteria associated with annual barley (Hordeum vulgare) and sweet clover (Melilotus albus) plants in an oil sands reclamation area. Our results suggest higher CYP153 gene copy numbers in sweet clover when compared to barley. Yet, no significant differences were detected in 16S rRNA, alkB and nah genes. In addition, total hydrocarbons, pH, total soil carbon, organic carbon and total nitrogen play an important role in determining hydrocarbon-degrading potential in these communities. The assessment of culturable hydrocarbon-degrading bacteria revealed 42 isolates (total of 316) that were positive for at least one hydrocarbon-degrading gene. Most of these isolates were positive for alkB, and closely match the database for Pantoea, Pseudomonas and Enterobacter spp. Thus, to improve oil sands reclamation strategies, plant inoculation with select hydrocarbon-degrading endophytes could be used to increase plant tolerance and hydrocarbon degradation in these areas.

Extent of pathogenic and spoilage microorganisms in whole muscle meat, meat products and seafood sold in Libyan market.

Background:Whole muscle meat, meat products, and seafood contain different nutrients in adequate quantity providing a better environment for presence and replication of different microorganisms. There are underreported and inaccurate estimations of foodborne diseases due to the lack of effective surveillance systems in Libya.Aim:To determine the extent of microbiological contamination of whole muscle meat, meat products, and seafood.Methods:A total number of 731 samples of retail meat were collected from different stores in four cities in Libya. Samples were analyzed for aerobic plate count and subjected to microbiological enumeration and isolation techniques, followed by molecular identification by PCR and partial sequencing of 16S rDNA.Results:The results showed contamination of samples with enteric and spoilage bacteria. Fifteen genera of spoilage bacteria yielded 149 isolates which were detected and identified by PCR and partial sequencing of 16S rDNA as: Proteus spp., Provedencia spp., Raouttella ornithinolytical, Citrobacter spp., Enterobacter spp., Morganella morgi, Shewanella algea, Rhodobacter capsulatus, Listonella pelagia, Kluyvera spp., Pectobacterium spp., Brenneria spp., Klebsiella spp., Acintobacter radioresistens, and Pantoea spp. While for pathogenic bacteria, 143 isolates distributed among nine genera were identified by PCR and partial sequencing of 16S rDNA as: Bacillus spp., Escherichia spp., Shigella spp., Enterococci spp., Cronobacter spp., Staphylococci spp., Salmonella spp., Aeromonas spp., and Vibrio spp.. Many isolated bacteria are zoonotic bacteria with high importance for public health.Conclusion:Excessive handling and processing of meat and meat products seems to be one of the poorest microbiological qualities. These findings ought to be helpful in risk assessments and quality assurance of meat in order to improve food safety.

Synthesis Characterization of new Nanoparticles Derived From Iron Oxide and Beta vulgaris extracts and its Bioactivity against Pantoea spp

This study discovers the opportunity of combining the clinical microbiology laboratories detection system with the VITEK 2 system to achieve rapid microbial identification and susceptibility testing of Pantoea spp. Susceptibility test results were compared for 3, 925 organism antibiotic combinations. The overall rate of categorical agreement of direct and conventional antimicrobial susceptibility testing was 92% with less than 3% very major and major errors combined. The mean turnaround time for identification and susceptibility testing was 7.5 hours (SD 3.0 hours) compared to a mean of 32.3 hours (SD 14.7 hours) for the conventional method. The present study concentrate on the antibacterial activity of Iron nanoparticle synthesized using Beta vulgaris extracts using a different concentrations (1 mg/ml, 2 mg/ml, 3 mg/ml, 4 mg/ml, against P ntoea spp.) and it was found that the highest concentration of Beta vulgaris iron nanoparticle gave the highest inhibition zone compare with the other samples.

Whole-Genome Sequence of a Pantoea sp. Strain Isolated from an Olive (Olea europaea L.) Knot.

Here, we present the total genome sequence of Pantoea sp. strain paga, a plant-associated bacterium isolated from knots present on olive trees grown on the Adriatic Coast. The genome size of Pantoea sp. paga is 5.08 Mb, with a G+C content of 54%. The genome contains 4,776 predicted coding DNA sequences (CDSs), including 70 tRNA genes and 1 ribosomal operon. Obtained genome sequence data will provide insight on the physiology, ecology, and evolution of Pantoea spp.

Molecular study of bacteria Pantoea spp. in Diwaniyah city, Iraq

Pantoea spp. possesses many virulent genes that help to cause many human infections. Therefore, the present study was comprehensive of clinical and environmental samples to determine the extent of the spread of Pantoea spp. in the hospitals in the city of Diwaniyah. A total of 623 samples were collected and distributed as483 samples of different clinical cases (77.52%) and 140 environmental samples (22.47%) from Diwaniyah city hospitals. The results showed that 24 isolates of Pantoea spp.s had been confirmed using API-20E Polymerase chain reaction technology. All isolates of Pantoea spp were tested for the prevalence of virulence genes (rcsB, hpaA, hrc, rcsA), using the polymerase chain reaction (PCR) technique. The highest incidence of hrc gene (70.83). The presence of rcsA gene was 68.42% with 13 isolates. The current study did not record any presence of the virulence genes( rcsB, hpaA) among the isolates of Pantoea spp.

ISOLATION AND IDENTIFICATION OF BRENNERIA NIGRIFLUENS AS CAUSAL AGENT OF BARK CANKER DISEASE ON WALNUT IN IRAQ

Bark canker disease has recently found in Sulaimani province. Disease incidence reached to 17.2% in Tawella. Bacterial isolates were identified by their morphological and biochemical characteristics using the API 20E system. Fifty-one and half percent of the isolates were identified as Brenneria nigrifluens and 36.3% as Pantoea spp. B. nigrifluens isolates formed circular colonies with entire margins and creamy color on nutrient agar. Biochemical tests classify B. nigrifluens isolates into seven groups. Vitek GN system further used to confirm the identification. The isolates produced necrotic lesion of different size on artificial inoculated walnut branches, but no symptoms on detached leaves. Isolates 22, 28 and 31 induced typical symptoms on two years old seedlings. B. nigrifluens successfully re-isolated from the inoculated seedlings. All B. nigrifluens isolates were resistant to erythromycin and cephalexin, most of them (16/17) were resistant to ampicillin, (13/17) resistant to vancomycin, (12/17) resistant to rifampin and amikacin; while (10/17) were moderately resistant to penicillin; (7/17) were moderately susceptible to streptomycin and (6/17) to gentamicin. Chloramphenicol, tobramycin, and tetracycline showed high efficiency in bacterial growth inhibition. Minimum inhibitory and minimum bactericidal concentrations of five chemicals against 17 bacterial isolates showed high efficiency of Kocide in killing 94.1% and inhibiting of 100% of the isolates at 0.31g/L In vitro, followed by Nordox which kill 70.6% and inhibit 76.4% of the isolates at 0.65 and 0.32 g/L respectively. In vivo studies confirm high efficacy of Kocide in disease control and restriction of vertical and horizontal extension of the cankers followed by Nordox.

Synthesis Characterization of new Nanoparticles Derived From Iron Oxide and Beta vulgaris extracts and its Bioactivity against Pantoea spp

This study discovers the opportunity of combining the clinical microbiology laboratories detection system with the VITEK 2 system to achieve rapid microbial identification and susceptibility testing of Pantoea spp. Susceptibility test results were compared for 3, 925 organism antibiotic combinations. The overall rate of categorical agreement of direct and conventional antimicrobial susceptibility testing was 92% with less than 3% very major and major errors combined. The mean turnaround time for identification and susceptibility testing was 7.5 hours (SD 3.0 hours) compared to a mean of 32.3 hours (SD 14.7 hours) for the conventional method. The present study concentrate on the antibacterial activity of Iron nanoparticle synthesized using Beta vulgaris extracts using a different concentrations (1 mg/ml, 2 mg/ml, 3 mg/ml, 4 mg/ml, against P ntoea spp.) and it was found that the highest concentration of Beta vulgaris iron nanoparticle gave the highest inhibition zone compare with the other samples.

Pantoea dispersa causing bulb decay of onion in Taiwan

Onion (Allium cepa L.) bulbs showing symptoms similar to those caused by slippery skin disease were sampled during a market survey in Chiayi city, Taiwan during 2017–2018. Interestingly, a bacterium which phenotypically differed from Burkholderia was isolated along with the slippery skin pathogen from the rotted scales. The purified isolates were initially identified as Pantoea dispersa by the phenotypic and biochemical characterization. Further molecular identification based on the 16S rDNA sequence analysis and multilocus sequence analysis with the atpD, gyrB, and infB sequences confirmed that the isolates are P. dispersa. For pathogenicity assay, surface-sterilized onion bulbs were injected with the isolates, and P. dispersa type strain LMG 2603T was included as a reference strain. Upon artificial inoculation of onion bulbs, all P. dispersa isolates and the strain LMG 2603T induced decays of the internal fleshy scales and yellow to tan discoloration similar to those caused by onion pathogenic Pantoea spp. The same bacterium was consistently re-isolated from the artificially inoculated onion bulbs, fulfilling Koch’s postulates. Moreover, a single injection of a P. dispersa suspension at a concentration of 1 × 104 CFU/mL was sufficient to cause disease. Accordingly, this study indicates, for the first time, that P. dispersa is an onion pathogen causing bulb decay in Taiwan.

Central Line-associated Pantoea spp. Bacteremia and Solid Organ Malignancy: A Report of Two Cases

Central Line-associated Pantoea spp. Bacteremia and Solid Organ Malignancy: A Report of Two Cases

Isolation, characterization and growth response study of chlorpyrifos utilizing soil bacterium Pseudomonas putida JR16

Agricultural pesticides are one of the indispensable materials used to meet increasing demand of food crops. Consequently, pesticides find their way to various ecosystems resulting in detrimental pollution problems. Chlorpyrifos is a predominantly used organophosphorus pesticide leading to harmful effects including abnormal cell division. Scientific attempt to eliminate these contaminants from the environment using biodegradation approach has been appreciated. Soil bacteria capable of utilizing chlorpyrifos are potential bioremediation candidates. In context to the same, the present study isolated six bacteria growing in presence of chlorpyrifos as sole carbon source from agricultural soils. The obtained bacterial isolates characterized based on their morphological characteristics, biochemical reactions were found to be Pseudomonas spp. JR16, Pseudomonas spp. J1, Pseudomonas spp. J2, Pantoea spp. J3, Enterobacter spp. J4 and Kocuria spp. J5. Among these, the most potential of them viz., Pseudomonas spp. JR16, was further proceeded for molecular identification and was observed to be the Pseudomonas putida JR16. The influence of different chlorpyrifos concentration, various carbon sources, metals sources, temperature and pH on the growth of Pseudomonas putida JR16 was assessed for developing optimum conditions for on field application of the strain for biodegradation of chlorpyrifos.