Abstract

Pantoea spp. possesses many virulent genes that help to cause many human infections. Therefore, the present study was comprehensive of clinical and environmental samples to determine the extent of the spread of Pantoea spp. in the hospitals in the city of Diwaniyah. A total of 623 samples were collected and distributed as483 samples of different clinical cases (77.52%) and 140 environmental samples (22.47%) from Diwaniyah city hospitals. The results showed that 24 isolates of Pantoea spp.s had been confirmed using API-20E Polymerase chain reaction technology. All isolates of Pantoea spp were tested for the prevalence of virulence genes (rcsB, hpaA, hrc, rcsA), using the polymerase chain reaction (PCR) technique. The highest incidence of hrc gene (70.83). The presence of rcsA gene was 68.42% with 13 isolates. The current study did not record any presence of the virulence genes( rcsB, hpaA) among the isolates of Pantoea spp.

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