ABSTRACT Within the framework of the Marie Curie FP7-PEOPLE-2013-ITN program, the ALErT project targets on tectonic and climatic boundary conditions in the regions along the densely populated in the Central Anatolian Plateau and the associated with natural hazards. The Central Anatolian Plateau (CAP) extends in a wide area in between zone the Aegean extensional zone and Bitlis /Zagros compressional zone. The Cankiri Basin was opened in Late Cretaceous between the Kirsehir block in the south and Sakarya continents in the north in central Anatolia. During the Neo-Tethys closure, the history of the basin became a large intermontane basin which covered with continental sedimentary environments (Kazanci et al., 1999). During the Late Miocene, there were different types of lacustrine environments in between the northern and southern parts. Age evidence of the Cankiri Basin has been obtained from the European mammalian faunal zones, because of the fact that Sr analysis results has not be significant to interpreted age (Mazzini 2015) and based on this, the late Messinian age Bozkir Formation and the Plio – Quaternary Degim Formation have seasonally dominant aridity. To figure out the paleoenvironmental and palaeoclimate changes in Cankiri Basin were analyzed Biotic (Paynology) and abiotic proxy data (geochemical, δ18O - δ13C isotopes analyses and CaCO3). Fifteen samples from Hancili Formation, Tuglu Suleymanli crossing border, Bozkir Formation and Degim Formation were collected from claystone, dark silty clay, gypsum and breccia with silty gypsum layers in the Cankiri Basin. The samples were treated standard palynological procedures described by Cour (1974). For pollen step all samples, residues, and slides are stored in Comenius University in Bratislava, Slovakia. Pollen grains for each sample were accounted 150 pollen grains, without Pinus in the Cankiri Basin. Pollen identification was performed under a Zeiss light microscope, and under a Quanta FEG250 Scanning electron microscope (SEM) used for high resolution imaging of pollen grains in Institute of Electrical Engineering Slovak Academy of Sciences. The pollen diagram was prepared with Tilia*Graph (2.0) (http://www.chrono.qub.ac.uk/datah/tilia.html). According to Mosbrugger and Utescher (1997); coexistence approach derived quantative paleoclimate parameters. To explain the ecological characteristic, we used version of the Past 3.x- the Past of the Future free software scientific statistical data analysis program (http://folk.uio.no/ohammer/past/). Head map was prepared with this program for four formations. Palynological data by evaluating head map, the uplift during the Late Messinian in Cankiri Basin has been confirmed. To illustrate for those vegetational composition, we applied Xact 8 for the graphic presentation. According to Traverse (1978) developed Steppe-Forest Index using a ratio of appropriate species of pollen, that save as a climatic indicator (warm to cold) on the other hand interpretation of aridity we follow Cour & Duzer (1978). As a consequence, the Poaceae/total Asteraceae ratio in a pollen diagram can be used as a climate index used Poaceae /Asteraceae ratio to find out dry to wet zones lines (Popescu, 2006) and it was calculated based on palynological results (using cluster analysis) show four climate cycles with three dry periods. Based on those calculations we confirmed long term cooling trend during Late Messinian to Plio-Pleistocene in Cankiri Basin. In most pollen spectra are mainly presented by Asteraceae, Chenopodiaceae, and Poaceae and by trees with Pinus, Cathaya , and Fagus. The group of herbs is important in the pollen sum and mainly consist of Poaceae, Chenopodiaceae and Asteraceae, which document open grassland type of vegetation with warm – temperate climate.