Palmitic Research Articles

Root exudate-mediated plant–microbiome interactions determine plant health during disease infection

Understanding the interactions between plants and microbes during pathogen infection is crucial for plant health improvement. However, the integrated role of soil nutrients, root exudates, and plant microbiomes in plant health during disease outbreaks remains unclear. In this study, we hypothesized that root exudate-mediated healthy microbiomes (HealthyBiome) would facilitate the escape of soil-borne pathogen infection, whereas a root exudate-mediated disease-conductive microbiome (PathoBiome) would induce disease occurrence. Using watermelon wilt disease caused by Fusarium oxysporum as a model, we investigated these interactions and their implications in plant disease. We examined the pathogen load across plant compartments during the pathogen accumulation (AH) and disease outbreak periods. We analyzed the plant microbiomes and root exudates during the outbreak, which was associated with the emergence in both healthy (BH) and diseased (BD) plants. Compared with AH and BD plants, BH plants had the lowest F. oxysporum density in the root–soil system (0.74–0.84 times that of AH and BD plants). BD plants showed an elevated amino acid metabolism (2.84–3.38 times that of healthy plants), whereas BH plants showed a higher metabolism of phenolic compounds (1.73 times that of diseased plants). BH plants secreted more palmitic acid (1.53 times that of diseased plants) and recruited more nitrogen (N)-fixing bacteria (e.g., Paenarthrobacter, Sphingomonas), which potentially alleviated N scarcity in the soil, promoted plant growth, and improved the plant defense. Structural equation modeling highlighted the interactions among soil nutrients, root metabolites, and plant nutrients in recruiting pro-growth bacteria to the rhizosphere and roots for healthy plant growth. Our findings provide novel evidence of how root exudates influence plant–microbiome interactions during pathogen infections, highlighting the importance of a balanced root exudate profile and its associated “HealthyBiome” in promoting plant health and growth.

Evaluation of Hepatic Glucose and Palmitic Acid Metabolism in Rodents on High‐Fat Diet Using Deuterium Metabolic Imaging

BackgroundOne of the main features of several metabolic disorders is dysregulation of hepatic glucose and lipid metabolism. Deuterium metabolic imaging (DMI) allows for assessing the uptake and breakdown of 2H‐labeled substrates, giving specific insight into nutrient processing in healthy and diseased organs. Thus, DMI could be a useful approach for analyzing the differences in liver metabolism of healthy and diseased subjects to gain a deeper understanding of the alterations related to metabolic disorders.PurposeEvaluating the feasibility of DMI as a tool for the assessment of metabolic differences in rodents with healthy and fatty livers (FLs).Study TypeAnimal Model.Population18 male Sprague Dawley rats on standard (SD, n = 9, healthy) and high‐fat diet (HFD, n = 9, FL disease).Field Strength/SequencePhase‐encoded 1D pulse‐acquire sequence and anatomy co‐registered phase‐encoded 3D pulse‐acquire chemical shift imaging for 2H at 9.4T.AssessmentLocalized and nonlocalized liver spectroscopy was applied at eight time points over 104 minutes post injection. The obtained spectra were preprocessed and quantified using jMRUI (v7.0) and the resulting amplitudes translated to absolute concentration (mM) according to the 2H natural abundance water peak.Statistical TestsTwo‐way repeated measures ANOVA were employed to assess between‐group differences, with statistical significance at P < 0.05.ResultsDMI measurements demonstrated no significant difference (P = 0.98) in the uptake of [6,6′‐2H2]glucose between healthy and impaired animals (AUCSD = 1966.0 ± 151.5 mM ‐ minutes vs. AUCHFD = 2027.0 ± 167.6 mM·minutes). In the diseased group, the intrahepatic uptake of palmitic acid d‐31 was higher (AUCHFD = 57.4 ± 17.0 mM·minutes, AUCSD = 33.3 ± 10.5 mM·minutes), but without statistical significance owing to substantial in‐group variation (P = 0.73).Data ConclusionDMI revealed higher concentrations of palmitic acid in rats with FL disease and no difference in hepatic glucose concentration between healthy and impaired animals. Thus, DMI appears to be a useful tool for evaluating metabolism in rodents with FL disease.Level of Evidence2Technical EfficacyStage 3.

Studies on Various Taxonomic, Pharmacognostic and Phytochemical Properties of Sanchezia speciosa Leonard Acanthaceae

Sanchezia speciosa Leonard, Acanthaceae is used as ornamental plant. It used in folklore medicine in the management of pain, anti-microbial and insecticide. An investigation was carried out to evaluate the various taxonomic, pharmacognostic and phytochemical standards to ensure the identity, purity, safety and efficacy of the medicinal plant, S. speciosa. Various observations were recorded which included microscopy, micromeritics, fluorescence, chemomicroscopy, soluble extractive values, moisture contents and phytochemical analysis. The results indicated that the epidermal shapes were both polygonal on the adaxial and abaxial surfaces. Stomatal distribution was amphistomatic with anisocytic and diacytic stomata on the adaxial and abaxial surface, respectively. Stomatal index was 21.47% on the adaxial surface and 15.43% on the abaxial surface. Micromeritics results for the leaf powder were bulk volume of 25.33± 0.408, tapped volume of 19.00± 0.00, bulk density of 0.40± 0.006, tapped density of 0.53± 0.021, Hausner’s ratio of 1.34± 0.037, Carr’s index of 25.00± 2.550 and angle of repose of 43.07°. The micromeritics indicated a passable flow. The chemo microscopy indicated presence of lignin, mucilage and starch. Fluorescence properties showed different colours under different ultraviolet lights. The water-soluble, methanol-soluble, ethanol-soluble extractive values were 14.7% w/w, 4.3% w/w and 5.0% w/w, respectively. Moisture content was 8.5% w/w. Total ash, acid-insoluble and water-soluble ash values were 20.0 % w/w, 2.0% w/w and 4.2% w/w, respectively. GC-MS of methanol extract revealed the total of 39 phytochemicals with over 5 prominent peaks having higher area% which include hexadecanoic acid methyl ester (2.64%), 9,12,15-octadecatrienoic acid methyl ester (4.77%), phytol (9.98%), 9,12,15 octadecatrienoic acid (12.97%), Stigmasterol (2.00%) and Squalene (28.16%). Other compounds are glycerin (5.83%), n-hexadecanoic acid (2.85%) and 9 octadecenamide (2.18%). Many of them possess good pharmacological properties. The data generated from the present study would help to authenticate S. speciosa and also affirm its folklore use in traditional medicine which has potential for further development into drug product.

Optimization, Production, and characterization of cottonseed methyl ester based on Box-Behnken in response surface design and gas Chromatography-Mass spectrum analysis

Renewable energy sources have a significant approach to eco-friendly systems with economically cost-effective and efficient use of biodiesel. The creation of innovative catalysts is attracting the attention and concern of the chemical and manufacturing sectors to be used in the production of biodiesel. The main objective of this work is to produce the optimum biodiesel yield using BBD design and the base-catalyzed method. The production of biodiesel was achieved through experimental investigation (alkali-catalyzed transesterification) and optimized using the BBD design method.The most important parameters such as methanol to oil ratios, KOH as catalysts, and reaction times were provided with variable conditions. The variation of methanol to oil ratios has significant relationships and is highly correlated in findings. The physicochemical properties of cottonseed biodiesel were carried out according to ASTM and EN standards. The maximum yield was achieved at 96.708 at a molar ratio of 6:1, reaction time of 60 min, and catalyst concentration of 1 wt%. The strong correlation between actual and predicted values of COSME was analyzed using ANOVA variances. The F-value and P-value validated the outcomes of produced biodiesel having a model value of 43.68 and 0.00, respectively. The coefficient of determinations and adjusted R2 of CSOME were achieved at 98.7% and 98.6%, respectively. The gas chromatogram mass spectrum analysis was also obtained to identify the acid profile present in COSME. The major percentage of ester available in COSME has been determined using GC–MS analysis and presented by its weight percentages. The main esters have been including myristic acid methyl ester (1.7%), palmitic acid methyl ester (26.65%), methyl 9-cis,11-trans-octadecadienoate(44.05%), ethyl 9. Cis.11.trans octadecadienoate(9.67%), methyl 20-methyl-heneicosanoate(6.83%), 9,12-Octadecadienoic acid (Z,Z)-, 2-hydroxy-1-(hydroxymethyl)ethyl ester(1.93%), and Pentacosanoic acid, methyl ester(7.14%).

Effect of pitavastatin on erythrocyte membrane fatty acid content in patients with chronic kidney disease: two-arm parallel randomized controlled trial.

Statins reduce the risk of cardiovascular events in patients with chronic kidney disease (CKD). Although diabetes mellitus (DM) is a reported side effect of statin treatment, some studies have indicated that pitavastatin does not cause DM. The present study investigated the effect of pitavastatin on the fatty acid (FA) content of erythrocyte membranes, which affects the occurrence of DM and cardiovascular diseases. In addition, changes in adiponectin and glycated hemoglobin (HbA1c) levels were evaluated after pitavastatin treatment. A total of 45 patients were enrolled, 28 of whom completed the study. Over 24 weeks, 16 patients received 2 mg pitavastatin and 12 patients received 10 mg atorvastatin. Dosages were adjusted after 12 weeks if additional lipid control was required. There were 10 and nine patients with DM in the pitavastatin and atorvastatin groups, respectively. Erythrocyte membrane FAs and adiponectin levels were measured using gas chromatography and enzyme-linked immunosorbent assay, respectively. In both groups, saturated FAs, palmitic acid, trans-oleic acid, total cholesterol, and low-density lipoprotein cholesterol levels were significantly lower than those at baseline. The arachidonic acid (AA) content in the erythrocyte membrane increased significantly in the pitavastatin group, but adiponectin levels were unaffected. HbA1c levels decreased in patients treated with pitavastatin. No adverse effects were associated with statin treatment. Pitavastatin treatment in patients with CKD may improve glucose metabolism by altering erythrocyte membrane AA levels. In addition, pitavastatin did not adversely affect glucose control in patients with CKD and DM.

Polycyclic Aromatic Hydrocarbons Contamination and Quality of Locally Produced Crude Palm Oil in South-South Zone, Nigeria

Background: Oil palm, Elaeis guineensis Jacq is a perennial crop mainly cultivated for its vegetable oil. The fatty acid composition of crude palm oil (CPO) is mostly composed of palmitic, stearic oleic, and linoleic acids. Objectives: This study evaluated the quality of locally produced CPO from six states of South-South zone, Nigeria. Methods: The parameters of CPOs were characterized by specific gravity (SG), acid value (AV), percentage of free fatty acid (%FFA), and saponification value (SV) and peroxide value (PV). Polycyclic aromatic hydrocarbons (PAHs) in CPO were quantified using standard procedures, the related risk due to daily consumption of 25 g was characterized by lifetime cancer risk (LCR) and margin of exposure (MOE). Results: The study observed that the mean values of the parameters were 0.931 SG; 5.01 mgKOH g-1 AV; 2.28% FFA; 177.27 mgKOH g-1 SV and 29.65 meqO2 kg-1 PV. Apart from %FFA, the others did not meet the requirements recommended by the Codex Alimentarius Commission of the joint WHO/FAO. Diagnostic ratios of PAHs suggested a mixture of petrogenic and pyrogenic origins. The MOEs showed low health concern while 16PAH-LCR values which ranged from 2.48E-04 - 4.48E-04 were unacceptable. The screening value 0.003 was significantly (p> 0.05) lower than the computed total BaP equivalent (BaPeq) values indicating potential human health concerns. It is recommended that CPO processing and storage methods should be properly monitored to prevent quality reduction and contamination, which might have adverse effect on consumers.

Phyto-constituents of the Dichloromethane Extracts of the Bulbs of Selected Genera in the Family Amaryllidaceae

The analysis of phytoconstituents present in the dichloromethane extract of the bulb of Hippeastrum vittatum (L’Her.) Herb., Proiphys amboinensis (L.) Herb., Urceolina amazonica (Linden) Christenh. & Byng., Hymenocallis littoralis (Jacq.) Salisb., Crinum jagus (J. Thomps.) Dandy and Zephyranthes carinata Herb. by Gas Chromatography-Mass Spectometry (GC-MS) analysis was carried out using standard methods. The Results of the GC-MS analysis of Hippeastrum vittatum, Proiphys amboinensis, Urceolina amazonica, Hymenocallis littoralis, Crinum jagus and Zephyranthes carinata revealed the presence of 50, 66, 61, 57, 56 and 61 peaks indicating the presence of the number of phytochemical constituents respectively. Phytol was present in Hymennocallis littoralis (0.41%) and Urceolina amazonica (0.79%) only. N-hexadecanoic acid, Di mevalonic acid lactone, Hexadecanoic acid methyl ester and Stigmasterol were present in all. Apiol and Xanthoxylin were absent in H. littoralis and P. amboniensis respectively. Oleic acid was present in Z. carinata (2.01%), P. amboniensis (1.85%) and U. amazonica (2.03%). Sakuranin was found in H. littoralis (3.42%), C. jagus (1.38%) and U. amazonica (4.43%). Linoleic acid ethyl ester was present in P. amboniensis (1.49%), U. amazonica (0.16%), H. littoralis (11.91%) and Z. carinata (0. 8 8%). The major components that delimited the species were as follows; In Hymenocallis littoralis was Liriodendromin (14.03%), Linoleic acid ethyl ester (11.91%) and Ethyl oleate (9.38%); in Zephranthes carinata; Phthalic acid, 4-methoxybenzyl methyl ester (15.34%) Gatanthamine, 3-O-acetyl-1, 2, dihydro (8.87%) Stigmasterol (5.87%); in Hippeastrum vittatum; N-benzyl-2-[1-(4-methoxyl-phenyl 1) – 1 H-tetrazo (14.94%), Benzaminde, 2-amino-N-(4-ethoxyphenyl) (12.65%) and 1-(5-methyl-2-hydroxyphenyl)-3-phenyl propane (10.49%); in Crinum jagus; 2H-1-Benzopyran-7-ol, 3,4-dihydro-3-(2-hyrdo) (19.87%), Benzamide, 2-amino-N- (4-ethoxyphenyl) (9.92%) and Stigmasterol (4.04%); in Proiphys amboniensis; Gamma-sitosterol (16.98%), Stigmasterol (11.49%) and N-Hexadecanoic acid(5.05%); in U. amazonica; 3-Furanacetic acid, 4-hexyl-2, 5-dihydro-2, 5 (12.73%), Benzamide, 2-amino-N- (4-ethoxyphenyl) (8.18%) and Phenyl, 2-(3,4-dihydro 2-methoxyl-2H-1-benzopy) (6.40%). The presence of various bioactive compounds may be responsible for the application of these species in the treatment and management of various ailments in folklore medicine. However, in vitro and in vivo studies, isolation of individual phytoconstituents and their mechanism of action may proceed to find a novel drug or lead compound for use as medicine.

Palmitic acid induces lipid droplet accumulation and senescence in nucleus pulposus cells via ER-stress pathway

Intervertebral disc degeneration (IDD) is a highly prevalent musculoskeletal disorder affecting millions of adults worldwide, but a poor understanding of its pathogenesis has limited the effectiveness of therapy. In the current study, we integrated untargeted LC/MS metabolomics and magnetic resonance spectroscopy data to investigate metabolic profile alterations during IDD. Combined with validation via a large-cohort analysis, we found excessive lipid droplet accumulation in the nucleus pulposus cells of advanced-stage IDD samples. We also found abnormal palmitic acid (PA) accumulation in IDD nucleus pulposus cells, and PA exposure resulted in lipid droplet accumulation and cell senescence in an endoplasmic reticulum stress-dependent manner. Complementary transcriptome and proteome profiles enabled us to identify solute carrier transporter (SLC) 43A3 involvement in the regulation of the intracellular PA level. SLC43A3 was expressed at low levels and negatively correlated with intracellular lipid content in IDD nucleus pulposus cells. Overexpression of SLC43A3 significantly alleviated PA-induced endoplasmic reticulum stress, lipid droplet accumulation and cell senescence by inhibiting PA uptake. This work provides novel integration analysis-based insight into the metabolic profile alterations in IDD and further reveals new therapeutic targets for IDD treatment.

Metabolomics revealed that toxic cyanobacteria stress reduced the flavor quality of Bellamya aeruginosa

The environmental impact of increased eutrophication and frequent cyanobacterial blooms on the growth and reproduction of aquatic organisms has become increasingly prominent in recent years. Bellamya aeruginosa is a nutrient-rich aquatic snail consumed by humans, and environmental changes may decrease its meat quality. However, the specific characteristics of muscle metabolism and flavor quality in freshwater snails and their response patterns to bloom-forming cyanobacterial stress have not been reported. Therefore, in this study, alterations in metabolites in the snail muscle after following feeding on toxic Microcystis aeruginosa were investigated using untargeted metabolomics. A total of 1,128 metabolites were identified, and the metabolic pathways of unsaturated fatty acid biosynthesis and purine, glutathione, and glycerophospholipid metabolism in snails fed toxic cyanobacteria differed from those in snails fed Chlorella vulgaris. Quantitative analysis showed increased levels of bitter-free amino acids, such as tyrosine, phenylalanine, and histidine, after consuming toxic cyanobacteria for 42 d, whereas a relative decrease was observed in the levels of umami- and sweet-free amino acids, such as glutamic acid, aspartic acid, serine, threonine, and glicine. The muscles of snails fed toxic cyanobacteria exhibited higher hypoxanthine and hypoxanthine nucleoside and lower adenosine triphosphate, adenine nucleotide, adenine nucleoside diphosphate, and hypoxanthine nucleotide contents than those of snails fed C. vulgaris. Furthermore, increased metabolites, such as stearic acid, palmitic acid, and cytidine diphosphate choline, and decreased metabolites, such as docosapentaenoic acid, docosahexaenoic acid, adrenoic acid, and L-glutamic acid, reflect the harmful effects of toxic cyanobacteria on the nutritional value and flesh quality of B. aeruginosa. This study comprehensively evaluated the effects of cyanobacterial blooms on freshwater gastropods, providing an important theoretical basis for the quality, safety, and sustainable development of snails as food and related processing industries.

Characterising bioactive components of green‐lipped mussel via two extraction methods: in vitro assessment of antioxidant and immunomodulatory effects

SummaryThis study investigates the benefits of green‐lipped mussels containing bioavailable PUFAs and inflammation‐modulating oil extracts. The goal is to find the best method for extracting lipids from two types of raw materials with strong immunomodulatory, anti‐inflammatory and antioxidant properties using organic solvents and supercritical carbon dioxide (CO2) techniques. The lipid classes are analysed using TLC‐FID and GC–MS to detect FFAs, while DPPH determines antioxidant levels. The cytokine production of IL‐6 and TNF‐α in LPS‐stimulated mouse macrophages is measured using ELISA. The results show that while supercritical CO2 is the most effective method for extracting lipids, the content can vary depending on the source and technique. The major FFAs found include EPA, Palmitic acid, DHA, and Palmitoleic acid, with PUFAs, particularly omega 3, being the most dominant. Samples exhibit potent radical scavenging in 2000 μg mL−1, similar to ascorbic acid at 62.5 μg mL−1 (62.2 ± 15.36%). The cells release more TNF‐α than IL‐6, and solvent extraction was found to regulate the immune response more effectively. In conclusion, the extracts contain significant amounts of EPA/DHA and variable FFAs, suggesting potential anti‐inflammatory effects. However, further validation in alternative in vitro models is necessary.

Erythrocyte Phospholipid Fatty Acid Profile in High-Level Endurance Runners

Fatty acids (FAs) are the major structural component of erythrocyte membranes. Diet and physical exercise directly influence their incorporation and function. Endurance runners engage in high volumes of weekly aerobic training, alternating between low-intensity and high-intensity sessions. The aim of the study was to assess and compare the erythrocyte FA profile in a group of high-level male endurance runners (EG) with a control group of non-athlete subjects (CG). This observational study was conducted on 85 subjects, 63 high-level male endurance runners (23 ± 3 years; height: 1.76 ± 0.05) and 22 subjects who did not engage in regular physical exercise (21 ± 0.5 years; height: 1.68 ± 0.39). Runners had at least five years of training experience, and all of them were participants in national and international tournaments. FAs determination was performed using gas chromatography. Higher percentages of Palmitic Acid (PA), Stearic Acid (SA), Oleic Acid (OA), Calendic Acid (CA), Eicosapentaenoic Acid (EPA) and Docosapentaenoic Acid (DPA), and lower percentages of Docosahexaenoic Acid (DHA) were found in the EG compared to the CG. High-level endurance runners exhibit altered erythrocyte FA profiles with low percentages of omega-3 index (ω-3 index) and DHA, which may affect erythrocyte membrane function as well as their performance.

The Impact of Deficit Irrigation on the Agronomic Performance and Chemical Composition of Scolymus hispanicus L.

In the current study, the effects of drought stress on the growth and phytochemical profile of Scolymus hispanicus L. (a.k.a. golden thistle) were evaluated. Plants were treated with three irrigation regimes, e.g., plants that received only rainwater (Control; C), deficit irrigation (I1; 50% of field capacity (FC)), and full irrigation (Ι2; 100% of FC). The fresh weight of the rosette of leaves was not negatively impacted by deficit irrigation, whereas root development was severely restrained compared to control and I2 treatments. Drought stress conditions had a positive effect on the nutritional properties of the golden thistle since the treatments of control and deficit irrigation showed the highest content of macronutrients and energy. Oxalic acid was the richest organic acid, especially under the I1 regime. Similarly, α-tocopherol was the only identified vitamin E isoform, whose content was also doubled in I1 treatment. Raffinose, glucose, and sucrose were the most abundant free sugars in amounts that varied among the irrigation treatments, while the total and distinct free sugar content was the highest for the I1 treatment. The most abundant detected fatty acid compounds were α-linolenic acid, followed by palmitic and linoleic acid, with the highest amount being detected in C, I1, and I2 treatments, respectively. Flavonoids were the only class of polyphenols detected in golden thistle leaves, including mostly kaempferol and quercetin derivatives. The greatest antioxidant potency was shown for the control and I1 treatments (for OxHLIA and TBARS methods, respectively). The evaluated leaf samples recorded a varied antimicrobial effect for the different bacterial strains and fungi, whereas no cytotoxic, hepatotoxic, and anti-inflammatory effects against the tested cell lines were recorded. Finally, the mineral content of leaves was significantly affected by the irrigation regime, with Ca, Mg, Cu, and Zn being the highest for the I1 treatment, while the I2 treatment had the highest content of K, Fe, and Mn and the lowest Na content. In conclusion, deficit irrigation showed promising results since it improved the phytochemical content without compromising the fresh weight of leaves, and thus it could be suggested as a sustainable agronomic practice for producing high-added value products without significant constraints in growth development and yield parameters of golden thistle.

In Vitro Bioactivities and In Silico ADME Profile for Potential Biological Target Prediction of Selected Phytochemicals Using Six Nepeta Species

AbstractSeveral Nepeta species, used in traditional uses, represent sources of valuable phytochemical composition, as well as in vitro biological and in silico clinical activities. In the present study, the antioxidant and DNA protective properties of methanol: chloroform (1 : 1) extracts of six Nepeta species were evaluated by comparison with standards. In HPLC‐MS/MS analysis, the main components of the extracts were identified as rosmarinic acid, caffeic acid, chlorogenic acid, shikimic acid, and scutellarin. In the GC‐FID analysis, the highest components were palmitate and cis‐11‐eicosenoic acid. N. aristata has the greatest total phenol and flavonoid contents. N. baytopii, N. aristata, N. italica, N. stenantha, and N. nuda exhibited the highest antioxidant activities. The N. nuda, N. aristata, N. italica, and N. trachonitica extracts were shown to have the most potent DNA protection activity. According to the result of the GC‐FID and HPLC‐MS/MS analysis of Nepeta extracts, ADMET studies of the main components of the extracts were performed. It was evaluated that palmitic acid had BBB permeability, but oleic acid was toxic. The in vitro and in silico results confirmed that Nepeta L. extracts are potential natural products.

Biosynthesis of Polyhydroxyalkanoates in Cupriavidus necator B-10646 on Saturated Fatty Acids.

It has been established that the wild-type Cupriavidus necator B-10646 strain uses saturated fatty acids (SFAs) for growth and polyhydroxyalkanoate (PHA) synthesis. It uses lauric (12:0), myristic (14:0), palmitic (16:0) and stearic (18:0) acids as carbon sources; moreover, the elongation of the C-chain negatively affects the biomass and PHA yields. When bacteria grow on C12 and C14 fatty acids, the total biomass and PHA yields are comparable up to 7.5 g/L and 75%, respectively, which twice exceed the values that occur on longer C16 and C18 acids. Regardless of the type of SFAs, bacteria synthesize poly(3-hydroxybutyrate), which have a reduced crystallinity (Cx from 40 to 57%) and a molecular weight typical for poly(3-hydroxybutyrate) (P(3HB)) (Mw from 289 to 465 kDa), and obtained polymer samples demonstrate melting and degradation temperatures with a gap of about 100 °C. The ability of bacteria to assimilate SFAs opens up the possibility of attracting the synthesis of PHAs on complex fat-containing substrates, including waste.

Impact of silver nanoparticles on secondary metabolite composition and toxicity in anise (Pimpinella anisum L.) callus culture

BackgroundThere are numerous challenges associated with producing desired amounts of secondary metabolites (SMs), which are mostly unique and cannot be chemically synthesized. Many studies indicate that nanoparticles (NPs) can boost the production of SMs. Still, the precise manner in which NPs induce metabolic changes remains unidentified. This study examines the influence of eco-friendly silver NPs (AgNPs) on the chemical makeup and toxicity of Pimpinella anisum L. (anise).ResultsAgNPs were introduced into anise callus cultures at different concentrations (0, 1.0, 5.0, 10, and 20 mg/L). The induced oxidative stress was tracked over intervals of 7, 14, 28, and 35 days. Chemical composition evaluations were carried out on the 35th day. Within the first 14 days, plant stress was evident, though the plant adapted to the stress later on. Notably, the plant showed high tolerance at 1 mg/L and 5 mg/L concentrations despite increased toxicity levels. However, relatively high toxicity levels were identified at 10 and 20 mg/L. The AgNP-induced stress significantly impacted anise SMs, particularly affecting fatty acid content. In the 10 and 20 mg/L AgNP groups, essential metabolites, including palmitic and linoleic acid, showed a significant increase. Polyunsaturated (omega) and monounsaturated fatty acids, vital for the food and pharmaceutical industries, saw substantial growth in the 1 and 5 mg/L AgNP groups. For the first time, vanillyl alcohol and 4-Hydroxybenzoic acid were detected along with various phenolic compounds, such as t-anethole, Salicylic acid, and Thiamazole.ConclusionAgNPs can function as an elicitor to efficiently generate essential SMs such as omegas and phenolic compounds in anise callus culture. This study explores the application of AgNPs as plant elicitors in anise SM production, offering invaluable insight into potential uses.

254 Effect of feeding high oleic soybean oil to finishing pigs on growth performance, carcass characteristics, and meat quality

Abstract The objective of this study was to evaluate the effects of dietary fat source and feeding duration on growth performance, carcass characteristics, and meat quality of finishing pigs. Finishing pigs [n = 450; 21 wk of age; initial body weight (BW) = 113.7 ± 8 kg] were housed in 90 pens and assigned to one of five dietary treatments in a 2 × 2 + 1 factorial design. Dietary treatments consisted of two fat sources (CWG: 4% inclusion of choice white grease and HOSO: 4% inclusion of high oleic soybean oil) each provided 2 or 4 wk before marketing. The “+1” diet was a corn-based diet without fat inclusion (CON). Observations included pig growth performance (on d 14 and d 28 of feeding), carcass characteristics (12 pigs per treatment at marketing), fatty acid profile in the loins and bellies, and a consumer sensory evaluation. Data were analyzed using PROC MIXED in SAS considering dietary treatment as a main effect, feeding duration, and their interactions. Contrast tests were used to compare dietary treatments with control group. From d 14 to 28 and overall experimental period (d 0–28), pigs fed fat-supplemented diets had a greater (P < 0.05) average daily gain and gain to feed ratio than CON fed pigs. There was no significant difference (P > 0.05) in growth performance when comparing fat sources or feeding periods. Pigs supplemented with either CWG or HOSO showed a tendency to have a greater (P < 0.1) belly weight and belly yield, and a lesser (P < 0.1) loin yield and loin muscle area when compared with CON pigs. Loins from the 2-wk period of fat supplementation had darker (Coloration l, P < 0.05) meat coloration when compared with 4 wk. The loin from pigs fed fat sources had greater (P < 0.05) concentration of oleic acid and eicosenoic acid when compared with CON. This improvement was mainly from HOSO. When CWG was compared with HOSO, pigs fed CWG had a greater (P < 0.05) concentration of palmitic acid and stearic acid while the HOSO pigs had a greater concentration of oleic acid and linolenic acid in the loin. In the belly, CON had greater (P < 0.05) concentration of palmitic acid and stearic acid while pigs fed fat sources had a greater concentration of oleic acid and eicosenoic acid. Bellies from HOSO had greater (P < 0.05) concentration of oleic acid and α-linolenic acid methyl ester while CWG had greater concentration of g-linolenic acid. As for the sensory evaluation, the palatability and acceptability of pork were not affected (P > 0.05) by dietary treatments. In conclusion, supplementation with HOSO not only improved performance but tended to improve some carcass characteristics and increased the concentration of oleic acid and some other unsaturated FAs with a concomitant decrease in the concentration of some saturated FAs in pork.

Glycyrrhizic acid attenuates the malignant biological properties of nonalcoholic fatty liver disease-related hepatocellular carcinoma.

Glycyrrhizic acid (GA) has effects on anti-hepatic fibrosis, anti-tumor and prevention from hepatocellular carcinoma (HCC) progression. Yet, the capacity of GA to ameliorate the advance of HCC pertinent to nonalcoholic fatty liver disease (NAFLD) remains to be clarified. We used the CCK-8 method to detect the optimal treatment concentration and time for L-02 cells, palmitic acid (PA)-induced L-02 cells and HepG2 cells, and selected 40 μM and 48 h to treat PA-induced L-02 cells and 60 μM for 24 h to treat HepG2 cells. Moreover, functional associations of HepG2 cells were elucidated through various assays. The results showed that GA demonstrated enhances lipid deposition and alleviates the inflammatory response in L-02 cells induced by palmitic acid. Simultaneously, we found that GA inhibits the proliferation, migration, and invasion while promoting apoptosis in HepG2 cells. In pursuit of constructing of HCC model rats, a combination of high-fat diets and diethylnitrosamine was utilized. The results showed that GA significantly decreased the liver index, body weight, liver weight, and the number of nodules in HCC model rats. Moreover, GA mitigated infiltration and heightened apoptosis in these rats. Mechanistically, GA notably attenuated the KKβ/NF-κB pathway in both HepG2 cells and the HCC model rats. In conclusion, GA functions as an inhibitor in the progression of NAFLD-related HCC cells, which might be relevant to the KKβ/NF-κB pathway. Therefore, GA is a potential drug for NAFLD-related HCC treatment.

Green biodiesel production from Nannochloropsis microalgae-derived oil using ZnAl-LDH catalyst: Process optimization and kinetic study

The green production of biodiesel using Nannochloropsis microalgae, a renewable and environmentally friendly source, was successfully conducted by application of a calcined ZnAl layered double hydroxides structure (LDH) catalyst. The ZnAl-LDO catalyst was prepared through the urea synthesis method, followed by calcination at 400 °C. XRD was used to investigate the crystallite characteristics, N2-BET to analyze surface properties and pore size, and CO2-TPD to identify the active basic sites strength. Bligh and Dyer’s method was utilized for oil extraction with a yield of 38 %. Upon analyzing the fatty acid profile of derived oil, a synthetic oil containing oleic and palmitic acids was utilized in this process. The experiments were designed using the response surface methodology (RSM) method and the optimized parameters were obtained at 149.59 °C, 118.92 min, 3.91 wt% catalyst loading, and 11.68:1 methanol to oil molar ratio for the biodiesel yield of > 98 %. The pseudo-first-order esterification reaction kinetic was investigated and the activation energy was determined to be 28.53 kJ.mol−1. Furthermore, the process can be applied to microalgae solid biomass as well. In-situ transesterification of microalgae with a yield of FAME over 90 % also has been carried out.

Heterogeneous hydrochlorination of lipids mediated by fatty acids in an indoor environment.

Fatty acids from cooking fumes and hypochlorous acid (HOCl) released from indoor cleaning adversely affect respiratory health, but the molecular-level mechanism remains unclear. Here, the effect of cooking oil fumes [palmitic acid (PA), oleic acid (OA), and linoleic acid (LA)] on lung model phospholipid (POPG) hydrochlorination mediated by HOCl at the air-water interface of the hanged droplets was investigated. Interfacial hydrochlorination of POPG was impeded by OA and LA, while that of POPG was facilitated by PA. The effect on POPG hydrochlorination increased with the decrease in oil fume concentration. A potential mechanism with respect to the chain length of these oil fumes, regardless of their saturation, was proposed. PA with a short carbon chain looses the POPG packing and leads to the exposure of the C=C double bonds of POPG, whereas OA and LA with a long carbon chain hinder HOCl from reaching the C=C bonds of POPG. These results for short chain and low concentration dependence suggest that the decay of oil fumes or the conversion of short-chain species by indoor interfacial chemistry might be adverse to lung health. These results provide insights into the relationship between indoor multicomponent pollutants and the respiratory system.

GC/MS chemical profiling and isolation of secondary metabolites from Leontice ewersmannii

GC/MS chemical profiling of the aerial parts of Leontice ewersmannii revealed the presence of various bioactive compounds of which 20 compounds were in n-hexane fraction and 3 compounds in basic DCM fraction. Among the identified compounds alkaloids, fatty acids and their esters, fatty alcohols, hydrocarbons, and sterol were detected. Phytochemical investigation of the aerial parts of L. ewersmannii led to isolation of 17 compounds including 1-heptacosanol (1), phytol (2), lupenone (3), palmitic acid (4), oleic acid (5), stigmasterol (6), stigmast-7-en-3-ol (7), methyl palmitate (8), methyl linoleate (9), butyl palmitate (10), butyl linoleate (11), dimethyl dodecanedioate (12), methyl cerebronate (13), β-sitosterol (14), β-sitosterol 3-O-β-glucopyranoside (15), sophoridine (16) and camoensine (17). The structures of the isolated compounds were determined by 1D NMR, HR-ESI-MS, and GC-MS. All methyl esters of fatty acids are possible artifacts during extraction. Additionally, the antimicrobial activity of the total extract and different fractions was screened, and they exhibited no inhibition of the growth at the tested concentration ranging from 8 to 200 μg/mL.