Oxidative Stress In Wistar Rats Research Articles

Subchronic Exposure to Mixture of Cadmium, Copper, and Nickel Induces Neurobehavioral Deficits and Hippocampal Oxidative Stress of Wistar Rats.

The present study was aimed at evaluating the influence of the subchronic exposure of cadmium (Cd), copper (Cu), and nickel (Ni) mixtures on affective behaviors, memory impairment, and oxidative stress (OS) in the hippocampus. Thirty male Wistar rats were divided into 5 equal groups. Group 1 (control) received a saline solution (NaCl 0.9%). Groups 2, 3, and 4 received Cd (0.25 mg/kg), Cu (0.5 mg/kg), and Ni (0.25 mg/kg), respectively, while group 5 received a Cd, Cu, and Ni mixture through intraperitoneal injections for 2 months. After the exposure period, all rats were submitted to behavioral tests. Subsequently, OS markers and histological changes in the rats' hippocampi were assessed. Results showed that a 2-month exposure to the mixtures of metals (MM) has led to higher anxiety-like and depression-like behaviors and cognitive deficits in rats when compared to the control group and the individual metals. Furthermore, the MM induced heightened OS, evidenced by the rise in lipid peroxidation and nitric oxide levels. These effects were accompanied by a decrease in superoxide dismutase and catalase activities in the hippocampus. The histopathological analysis also supported that MM caused a neuronal loss in the CA3 sub-region. Overall, this study underscores that subchronic exposure to the Cd, Cu, and Ni mixture induces an OS status and histological changes in the hippocampus, with important affective and cognitive behavior variations in rats.

Dietary snakehead fish powder alleviates renal injury by suppressing oxidative stress in rat gentamicin-induced

Nephrotoxicity or renal injury is triggered due to the accumulation of drug levels in the kidneys causing oxidative stress. Albumin and omega-3 are known to repair and protect against tissue damage by free radicals due to oxidative stress. Snakehead fish (Channa striata) is a natural resource that is rich in albumin and omega-3. This study aimed to determine the nephroprotective activity of snakehead fish powder (SFP) in Wistar rats induced by gentamicin. This study used thirty male rats divided into six groups. Test animals were divided into normal control (not treated); positive control (omega-3 270 mg/ kg of rat); negative control (CMC Na 0.25%); dose I (350 mg/kg SFP); dose II (700 mg/ kg SFP) and dose III (1400 mg/kg SFP). All groups were induced with 100 mg/kg gentamicin intraperitoneally except for normal control. The treatment was carried out for 14 days. Rats fasted for 24 hrs after the treatment on the last day. The blood test animals were taken to measure creatinine, urea and albumin levels, then sacrificed, and their kidneys were taken for analysis of malondialdehyde (MDA) and glutathione (GSH) levels. The data obtained were statistically tested using the one-way ANOVA method and continued with post hoc LSD. The results showed the effect of SFP on the decrease in creatinine, urea, MDA, and GSH levels starting from the smallest dose of 350 mg/kg significantly compared with the negative control in gentamicin-induced Wistar rats. A higher dose of SFP can provide better nephroprotective activity. In conclusion, SFP has nephroprotective activity by suppressing oxidative stress in Wistar rats.

Phœnix dactylifera, L. seed oil alleviates Bleomycin-induced pulmonary fibrosis and oxidative stress in Wistar rats

Objective Idiopathic pulmonary fibrosis (IPF) is the most serious form of interstitial lung disease. We aimed to investigate the effect of Phœnix dactylifera, L. seed oil (DSO) on a murine model of IPF induced by bleomycin (BLM). Methods Male Wistar rats were treated with a single intra-tracheal injection of BLM (4 mg/kg) and a daily intraperitoneal injection of DSO (75, 150 and 300 mg/kg) for 4 weeks. Results Our phytochemical results showed that DSO has an important antioxidant activity with a high content of polyphenols and flavonoids. High-Performance Liquid Chromatography (HPLC) and Gas chromatography/mass spectrometry (GC-MS) analysis revealed a high amount of oleic and lauric acids and a large quantity of vitamins. Histological examination showed a significant reduction in fibrosis score and collagen bands in the group of rats treated with 75 mg/kg of DSO compared to the BLM group. DSO (75 mg/kg) reversed also the increase in catalase and malondialdehyde (MDA) levels while higher doses (150 and 300 mg/kg) are ineffective against the deleterious effects of BLM. We revealed also that DSO has no renal or hepatic cytotoxic effects. Conclusion DSO can play antioxidant and antifibrotic effects on rat models of pulmonary fibrosis at the lowest dose administered.

Effects of Energy Drinks and Flunitrazepam on Some Cardiac Biomarkers, and Oxidative Stress in Wistar Rats.

This study was conducted to investigate the effects of some energy drinks on cardiac biomarkers (tropin and creatinine phosphokinase) and oxidative stress in male Wistar rats. 45 Wistar rats were divided into 8 groups. Group 1 received distilled water; Group 2 received an energy drink (A) (3.75 mg/kg). Group 3 energy drink (A) (7.5 mg/kg) Group 4 received energy drink (B) (3.75 mg/kg). Group 5 energy drink (B) (7.5 mg/kg) Group 6 received flunitrazepam (0.03 ml/kg), Group 7 received 3.75 ml/kg of energy drink (A) and 0.03 ml/kg of flunitrazepam, and Group 8 received 3.75 ml/kg of energy drink (B) and 0.03 ml/kg of flunitrazepam. The result showed that in Group 2, there was a significant increase (p<0.05) in cardiac troponin kinase and no significant change in bicarbonate. Group 3 showed a significant increase (p<0.05) in the level of cardiac troponin, a significant increase (p<0.05) in creatine kinase, and no significant change in bicarbonate. Group 4 showed a significant (p > 0.05) decrease in cardiac troponin and creatine kinase and no significant change in bicarbonate. Group 5 showed a significant (p > 0.05) decrease in cardiac troponin and creatine kinase and no significant change in bicarbonate when compared to the control group. Group 6 showed a significant (p<0.05) increase in cardiac troponin and a decrease (p>0.05) in creatine kinase, but no significant change in bicarbonate. Group 7 showed a significant decrease in cardiac troponin and creatine kinase and no significant change in bicarbonate level when compared to the control group. Group 8 showed a decrease in cardiac troponin and creatine kinase. It was concluded therefore that the consumption of energy drinks and Flunitrazepam led to an increase in oxidative stress and cellular damage.

The Effect of Chronic Administration of Amphetamine-Type Stimulants on Oxidative Stress and Inflammation in Wistar Rats

This study aims to determine the effect of chronic administration of amphetamine-type stimulants at varying doses on inflammation and oxidative stress in Wistar rats. They were given methylphenidate and divided into 4 treatment groups. Furthermore, simple random grouping was carried out to divide the samples into 5 groups, each consisting of 9 rats. These groups included rats given only distilled water as the controls, as well as those given methylphenidate at doses of 10 mg/kg BW, 20 mg/kg BW, and 40 mg/kg BW for 4 weeks as the experimental groups. Statistical analysis was then performed using GraphPad Prism to compare the effects of oxidative stress, systemic inflammation, BDNF, and spatial memory of the Wistar rats. Chronic administration of stimulants led to a significant decrease in glutathione peroxidase levels as well as an increase in the release of IL-6 and tumor necrosis factor-α compared to the control group. Based on the results, the pathways involved in cognitive impairment, which were related to the use of amphetamine-type stimulants played a role in addressing the detrimental effect of substance abuse and their comorbidities

Modulatory effect of Justicia secunda leaf extract on hematological status, lipid profile, liver function and oxidative stress in Wistar rats

Modulatory effect of Justicia secunda leaf extract on hematological status, lipid profile, liver function and oxidative stress in Wistar rats

Effect of peat biomass smoke exposure on oxidative stress in Wistar rats

Background: Indonesia ranks third in the world regarding air pollution due to forest and land fires; most of the land burned is a peatland. Particulate matter (PM) 2.5 is the largest component of the total smoke particles. Short-term and long-term exposure to PM2.5 remains a hazard to human health.Objective: This study aims to examine the effect of exposure to peat biomass smoke on serum malondialdehyde (MDA) levels and body weight of Wistar rats.Methods: Experimental animals were randomly divided into three groups: Control group (C) is not given treatment, and treatment groups (X1 and X2) are exposed to smoke from peat biomass of 100 g and 150 g of biomass for 60 seconds per day for 14 days. The body weight was examined before and after treatment, while Serum MDA levels were examined after treatment.Results: The results showed significant differences (p < 0.05) in serum MDA levels between groups. The highest serum MDA levels were found in group X2 (3.03 ± 0.185 nmol/ml), followed by group X1 (2.67 ± 0.212 nmol/ml) compared to the control group (2.24 ± 0.476 nmol/ml). In contrast, increasing body weight between groups did not show a significant difference.Conclusion: Exposure to PM 2.5 from peat biomass smoke increases oxidative stress in experimental animals.

Oxidative Stress in Wistar Rats Under Acute Restraint Stress and Its Modulation by Antioxidants and Nitric Oxide Modulators.

Several pathogenic conditions leading to morbidity, including cancer, aging, diabetes, reperfusion injury, cardiovascular disease, and neurological disorders, are known to be exacerbated by oxidative stress. Antioxidant therapy is effective in the treatment of such disorders and appears to be a potential therapeutic technique to reduce oxidative stress. The aim of our study is to investigate the antioxidant effects of L-ascorbic acid and nitric oxide (NO) modulators on rats suffering from oxidative stress induced by acute restraint stress (RSx1). In this in vivo study, Wistar rats were subjected to one hour of restraint stress on day 21 to induce oxidative stress. Superoxide dismutase (SOD), total antioxidant capacity (TAC), catalase, glutathione (GSH), and malondialdehyde (MDA) were used to assess the antioxidant effects. IBM Corp. Released 2013. IBM SPSS Statistics for Windows, Version 22.0. Armonk, NY: IBM Corp. was used for data analysis. Compared to vehicle groups, acute restraint stress (RSx1) dramatically increased MDA levels while decreasing GSH, SOD, total antioxidant capacity, and catalase. L-NAME, 7-NI, AG (50 mg/kg each), and L-ascorbic acid (200 mg/kg) reversed the changes in SOD, MDA, GSH, total antioxidant capacity, and catalase levels. The NO precursor L-arginine (1000 mg/kg) and NO synthase inhibitors followed the same trend. Our study findings highlight the complex role of antioxidants and NO modulators in the pathogenesis of diseases, as evidenced by the reversal of oxidative stress indicators. Antioxidant therapy, with its potential to mitigate oxidative stress, emerges as a viable treatment option for a range of pathological conditions associated with oxidative stress.

Protective effect of vitamin C against ethanol induced oxidative stress in Wistar rats

Inflammation is a natural immune response to harmful agents. This study aimed to explore the protective effect of vitamin C against alcohol-induced toxicity on hematological inflammatory markers. Forty healthy adult male Wistar rats were acclimatized and divided into eight groups. Group A served as the control, while Group B received alcohol. Groups C, D, and E were given varying doses of vitamin C, and Groups F, G, and H received alcohol followed by vitamin C treatments. After twenty-one days, blood samples were collected and analyzed for various markers. Rats receiving alcohol only showed increased white blood cell count (WBC), platelet count (PLT), neutrophil to lymphocyte ratio (NLR), and platelet to lymphocyte ratio (PLR), along with decreased antioxidant enzyme activity. However, rats receiving vitamin C alone or in combination with alcohol exhibited reduced inflammatory markers and increased antioxidant activity compared to the alcohol-only group. The study demonstrated that commercial-grade vitamin C at doses of 100 mg/kg, 200 mg/kg, and 300 mg/kg effectively reduced chronic low-grade inflammation. Overall, the findings suggest that vitamin C supplementation may mitigate the inflammatory effects of alcohol consumption.

Potential role of Vitamin C as an Antioxidant on Bisphenol (BPA) Induced Oxidative Stress in Wistar rats

Potential role of Vitamin C as an Antioxidant on Bisphenol (BPA) Induced Oxidative Stress in Wistar rats

Effects of Oral Administration of Pulegone on Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

Effects of Oral Administration of Pulegone on Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

Combination of Curcumin and Quercetin: Reclaiming the Lost Ground Against Nephrotoxicity

Background: An alkylating agent named cyclophosphamide (CP) is an immunosuppressant used in the therapy of rheumatoid arthritis and various cancers. The combination of curcumin and quercetin was evaluated for antioxidant activity against CP-induced renal oxidative stress in Wistar rats. Methods: A prophylactic treatment using a combination of curcumin and quercetin is given to the rats at 80 mg/kg and 200 mg/kg (b.w.) of the oral dose administered before single injection of CP at 200 mg/kg intra-peritoneally (i.p.). The effects of curcumin and quercetin combination on CP-induced nephrotoxicity were investigated using the assay of oxidative stress biomarkers, serum kidney toxicity markers, and histopathology of kidney tissue. Result: A single dose of CP were enhanced the malondialdehyde (MDA), creatinine, blood urea nitrogen (BUN) level and reduced the body weight indices (OBWI), haematological parameter, glutathione (GSH) content. The oral administration of curcumin and quercetin were caused a substantial reduction of the MDA, creatinine, BUN level and increased the OBWI, haematological parameter, GSH content. Conclusion: The present findings suggest that curcumin and quercetin combination has a prominent role against CP-induced renal injury.

PS-B05-12: ANTIHYPERTENSIVE AND CARDIO-PROTECTIVE POTENTIAL OF O. SANCTUM LEAVES EXTRACT IN L-NAME INDUCED HYPERTENSIVE RATS

Objective: There are approximately 26% of adults in the world who suffer from hypertension, which is the most common cardiovascular disease. Essential hypertension is mainly caused by endothelial dysfunction which results from nitric oxide (NO) deficiency. The present study sought to determine the impact of O. sanctum leaf extract (OS) in protecting against L-NAME induced hypertension and oxidative stress in wistar rats. Design and method: A non-specific nitric oxide inhibitor, L-NAME, (40 mg/kg/day), was administered to male Wistar rats to induce hypertension. CoQ10 was used as a standard anti-hypertensive compound. All of the other groups received L-NAME combined with OS extract (200 mg/kg/day) orally for three weeks. Following the treatment, hemodynamic parameters were measured using direct cannulation. The lipid profile, kidney and cardiac functions, as well as markers of oxidative stress were evaluated. The results were expressed as mean ± SD. Results: L-NAME was found to cause high blood pressure, NO depletion, oxidative imbalance and damage in cardiac and kidney function in animals. The Cardiac markers enzyme level were significantly increased in L-NAME only group whereas, the CO-treatment group had lower lower of Cardiac markers as compared to control as well as L-NAME group. In hypertensive rats, OS treatment significantly lowers the expression of angiotensin converting enzyme (ACE) and mineralocorticoid receptor expression in hypertensive rats. Concurrent treatment with L-NAME and OS extract prevented the all injuries without affecting the heart rate or raising blood pressure. Conclusions: The results confirm O. Sanctum leaves extract antihypertensive action and indicate its protective properties in the L-NAME induced hypertension model of rats, likely due to the presence of rich antioxidant potential and by inhibiting the inflammation.

PS-C14-11: COFFEA ARABICA SEEDS EXTRACT HAS ANTIHYPERTENSIVE AND CARDIOPROTECTIVE EFFECTS IN L-NAME-INDUCED HYPERTENSIVE RATS

Objective: A large percentage of the adult population worldwide is affected by hypertension, which is the most common cardiovascular disease. Essential hypertension is primarily caused by an absence of nitric oxide (NO), which leads to endothelial dysfunction. This study examined whether Coffea Arabica seeds extract (CA) was effective against L-NAME-induced hypertension and oxidative stress in wistar rats. Method: Approximately 40 mg/kg of L-NAME, a non-specific nitric oxide inhibitor, was administered to male Wistar rats to induce hypertension in them, while CoQ10 served as a standard anti-hypertensive agent. L-NAME was administered orally to all of the other groups along with the CA extract for three weeks. A direct cannulation procedure was used to measure hemodynamic parameters after the treatment. The lipid profile, kidney and cardiac functions along with markers of oxidative stress were examined, as well. Result: It has been found that L-NAME increases blood pressure, depletes NO, causes oxidative imbalance, and damages cardiac and kidney function in animals. As compared to control and L-NAME groups, the CO-treatment group had significantly lower levels of cardiac markers than the L-NAME only group. The CA treatment of hypertensive rats significantly reduced the levels of angiotensin converting enzyme (ACE) and mineralocorticoid receptor expression. It was found that when L-NAME and CA extract were administered concurrently, all injuries were prevented without affecting the heart rate or raising the blood pressure. Conclusion: This study confirms the antihypertensive action of Coffea Arabica seeds extract and indicates its protective properties in a L-NAME induced hypertension model in rats, most likely due to the presence of antioxidant potential and by inhibiting inflammation in the tissues.

Antioxidant Activity Of Ajaswagandhadhi Lehyam Against D-Galactosamine Induced Oxidative Stress In Rats

D-Galactosamine is one among the most experimentally used drug to study hepatotoxic effects in experimental animals. The model system of liver damage produced by D-galactosamine in rats is recognized to be much like viral hepatitis in humans from both morphological and functional points of view. It also causes oxidative stress in rats. Ajaswagandhadhi Lehyam has ingredients that reduce oxidative stress. This study deals antioxidant activity of Ajaswagandhadhi Lehyam against D-galactosamine induced oxidative stress in wistar rats.

Antioxidant status, antihyperlipidemic activity, and serum electrolyte levels in dexamethasone-induced oxidative stress in Wistar rats treated with vitamin E

Antioxidant status, antihyperlipidemic activity, and serum electrolyte levels in dexamethasone-induced oxidative stress in Wistar rats treated with vitamin E

Irvingia gabonensis leaf extract scavenges nitric oxide and hydrogen peroxide in vitro and modulates arsenic-induced hepatic oxidative stress in wistar rats

BackgroundArsenic is a carcinogenic heavy metal that contaminates the environment, predisposing the exposed populace to its detrimental health effects. This study investigated the liver protective effect of ethanol leaf extract of Irvingia gabonensis (ELEIG) in sodium arsenite (SA)-exposed Wistar rats and its nitric oxide (NO.) and hydrogen peroxide (H2O2)-scavenging properties in vitro.MethodsEleven experimental groups made up of five (5) rats each (weight range 100 - 161 g) were used in this study. Group 1 (normal control) had normal rat chow and water. Group 2 received 4.1 mg/kg body weight (kgbw) of SA. Groups 3–8 received SA and graded doses of ELEIG and groups 9-11 had varied doses of ELEIG. Treatment, which spanned 14 days, was by oral gavage. Concentrations of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) as well as activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total bilirubin (TBIL) and direct bilirubin (DBIL) were determined using standard procedures. Standard methods were also used to determine the in vitro NO. and H2O2-scavenging properties of the extract.ResultsExposure to SA orchestrated significant (p ˂ 0.05) increases in CAT, MDA, AST, ALT, ALP and GGT and significant (p ˂ 0.05) decreases in SOD and GPx, relative to control. There were insignificant (p ˃ 0.05) differences in TBIL and DBIL concentrations, compared with control. Simultaneous and post-treatment with ELEIG at graded doses, alleviated the noxious effects of SA. In addition, ELEIG scavenged NO. and H2O2 in concentration-dependent manner.ConclusionThe results suggest that ELEIG possesses potent antioxidant property and combats SA-induced hepatic oxidative stress/toxicity in Wistar rats.

Methanol leaf extracts of Chrysophyllum albidum and Irvingia gabonensis protected against dyslipidaemia and oxidative stress induced by high-fat diet in Wistar rats

BackgroundHigh-fat diet (HFD) has been linked to oxidative stress, dyslipidaemia, obesity and cardiovascular diseases. Studies have shown that medicinal plants have antioxidant properties and may have protective effects against oxidative stress and dyslipidaemia induced by high-fat diet. Chrysophyllum albidum (white star apple) and Irvingia gabonensis (African bush mango) are very useful medicinal plants common in the tropical and subtropical regions of the world. This study was aimed at investigating the protective effect of methanol leaf extracts of Chrysophyllum albidum and Irvingia gabonensis against dyslipidaemia and oxidative stress in Wistar rats promoted by the consumption of HFD as well as characterize active compounds in the extracts. Thirty-six male Wistar rats were assigned into six groups of six animals each and respective groups received normal fat diet (NFD), HFD, HFD + Chrysophyllum albidum (250 mg/kg/ 500 mg/kg), HFD + Irvingia gabonensis (250 mg/kg/ 500 mg/kg) for fifty six days. Dietary intake, body weight, lipid profile and indicators of oxidative stress were measured.ResultsAdministration of plant extracts did not change the pattern of food intake of the animals. There was a significant inhibition (p < 0.05) of increase in the body weight of rats treated with plant extracts in comparison with those rats that consumed HFD only. There was significant increase (p ˂ 0.05) in total cholesterol, triacylglycerol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol and malondialdehyde concentrations in rats fed with HFD only when compared with NFD control and extract treated groups. However, HFD control produced significantly lower (p < 0.05) high-density lipoprotein cholesterol, reduced glutathione and glutathione peroxidase than normal control and extract treated groups. Gas chromatography flame ionization detector analysis of these extracts revealed the abundance of kaempferol, quercetin, luteolin, myricetin and naringenin and tannic acid.ConclusionsThe observed antioxidant and anti-dyslipidaemic properties of leaves of Chrysophyllum albidum and Irvingia gabonensis may be attributed to the presence of flavonoids and tannic acid.

Protective Effects of Wine Polyphenols on Oxidative Stress and Hepatotoxicity Induced by Acrylamide in Rats.

In recent years, it has been increasingly suggested that the consumption of natural polyphenols, in moderate amounts, is beneficial for health. The aim of this study was to investigate the efficacy of a red wine (the administered dose of 7 mL/kg/day being equivalent to ~16.5 mg/kg/day total polyphenols) compared to a white wine (the administered dose of 7 mL/kg/day being equivalent to ~1.7 mg/kg/day total polyphenols), on the prevention of acrylamide-induced subacute hepatic injury and oxidative stress in Wistar rats. Hepatic damage due to acrylamide intoxication (the administered dose being 250 µg/kg body weight, for 28 days, by intragastric gavage) was assessed by employing biochemical parameters (aspartate aminotransferase (AST) and alanine aminotransferase (ALT)) and by histopathological studies. Markers of oxidative damage were measured in terms of plasma malondialdehyde (MDA), hepatic Thiobarbituric Acid Reactive Substances (TBARS) and glutathione (GSH) levels, and liver antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)) activities. Regarding hepatic enzyme activities, treatment with red wine significantly decreased the AST values (p < 0.05), while for the ALT values only a normalization tendency was observed. Treatment with red wine and white wine, respectively, significantly prevented the increase in MDA and TBARS levels (p < 0.05), as well as the depletion of GSH (p < 0.05). Red wine treatment normalized the activities of the antioxidant enzymes CAT and SOD in rats intoxicated with acrylamide, while supplementing the diet with white wine did not produce significant differences in the antioxidant enzyme activities. Histopathological findings revealed a moderate protective effect of red wine after four weeks of daily consumption. Our findings provide evidence that red wine, having a higher phenolic content than white wine, has a significant protective effect on oxidative stress and liver injury induced by acrylamide in rats, through its antioxidative activity.

Effects of Phoenix dactylifera Tree Fruit Extract on Cadmium Induced Renal Damage in Adult Wistar Rats

The objective of the study is to investigate the ameliorative potential of date palm tree (Phoenix dactylifera) fruit on cadmium induced kidneydamage in adult Wistar Rat using standard technique. Thirty (30) adult Wistar rats were divided into six groups of five rats per group. Group A (control); Group B (0.1ml/kg body weight of cadmium); Group C (200mg/kg body weight of Phoenix dactylifera); Group D (600mg/kg body weight of Phaoenix dactylifera); Group E (0.1ml/kg body weight of cadmium and 200mg/kg body weight of Phoenix dactylifera); Group F (0.1ml/kg body weight of cadmium and 600mg/kg body weight of Phoenix dactylifera). Treatments were administered once daily for 30 days. After day 30, biochemical and histopathological studies were conducted to appraise oxidative stress and renal toxicity. Serum levels of creatinine, urea, catalase (CAT) and superoxide dismutase (SOD) were quantified. Animals treated alone with cadmium (Group B), showed significant (P &lt; 0.05) increase in serum urea, serum creatinine and CAT, SOD levels statistically. Treatment of rats with Phoenix dactyliferaalongside cadmium (Group E and F) showed significant (P &lt; 0.05) improvement in renal function and restoration of biochemical parameters mentioned earlier. Histopathological findings confirm these conservations. Therefore, the administration of aqueous extract of Phoenix dactylifera has protective effect on cadmium-induced renal toxicity and oxidative stress in wistar rats which can be attributed to Phoenix dactylifera antioxidant activity.