Dietary snakehead fish powder alleviates renal injury by suppressing oxidative stress in rat gentamicin-induced

Abstract

Nephrotoxicity or renal injury is triggered due to the accumulation of drug levels in the kidneys causing oxidative stress. Albumin and omega-3 are known to repair and protect against tissue damage by free radicals due to oxidative stress. Snakehead fish (Channa striata) is a natural resource that is rich in albumin and omega-3. This study aimed to determine the nephroprotective activity of snakehead fish powder (SFP) in Wistar rats induced by gentamicin. This study used thirty male rats divided into six groups. Test animals were divided into normal control (not treated); positive control (omega-3 270 mg/ kg of rat); negative control (CMC Na 0.25%); dose I (350 mg/kg SFP); dose II (700 mg/ kg SFP) and dose III (1400 mg/kg SFP). All groups were induced with 100 mg/kg gentamicin intraperitoneally except for normal control. The treatment was carried out for 14 days. Rats fasted for 24 hrs after the treatment on the last day. The blood test animals were taken to measure creatinine, urea and albumin levels, then sacrificed, and their kidneys were taken for analysis of malondialdehyde (MDA) and glutathione (GSH) levels. The data obtained were statistically tested using the one-way ANOVA method and continued with post hoc LSD. The results showed the effect of SFP on the decrease in creatinine, urea, MDA, and GSH levels starting from the smallest dose of 350 mg/kg significantly compared with the negative control in gentamicin-induced Wistar rats. A higher dose of SFP can provide better nephroprotective activity. In conclusion, SFP has nephroprotective activity by suppressing oxidative stress in Wistar rats.