Effects of Energy Drinks and Flunitrazepam on Some Cardiac Biomarkers, and Oxidative Stress in Wistar Rats.

Abstract

This study was conducted to investigate the effects of some energy drinks on cardiac biomarkers (tropin and creatinine phosphokinase) and oxidative stress in male Wistar rats. 45 Wistar rats were divided into 8 groups. Group 1 received distilled water; Group 2 received an energy drink (A) (3.75 mg/kg). Group 3 energy drink (A) (7.5 mg/kg) Group 4 received energy drink (B) (3.75 mg/kg). Group 5 energy drink (B) (7.5 mg/kg) Group 6 received flunitrazepam (0.03 ml/kg), Group 7 received 3.75 ml/kg of energy drink (A) and 0.03 ml/kg of flunitrazepam, and Group 8 received 3.75 ml/kg of energy drink (B) and 0.03 ml/kg of flunitrazepam. The result showed that in Group 2, there was a significant increase (p<0.05) in cardiac troponin kinase and no significant change in bicarbonate. Group 3 showed a significant increase (p<0.05) in the level of cardiac troponin, a significant increase (p<0.05) in creatine kinase, and no significant change in bicarbonate. Group 4 showed a significant (p > 0.05) decrease in cardiac troponin and creatine kinase and no significant change in bicarbonate. Group 5 showed a significant (p > 0.05) decrease in cardiac troponin and creatine kinase and no significant change in bicarbonate when compared to the control group. Group 6 showed a significant (p<0.05) increase in cardiac troponin and a decrease (p>0.05) in creatine kinase, but no significant change in bicarbonate. Group 7 showed a significant decrease in cardiac troponin and creatine kinase and no significant change in bicarbonate level when compared to the control group. Group 8 showed a decrease in cardiac troponin and creatine kinase. It was concluded therefore that the consumption of energy drinks and Flunitrazepam led to an increase in oxidative stress and cellular damage.