Oxidation Products Research Articles

Method validation and characterization of stress degradation products of azelastine hydrochloride using LC-UV/PDA and LC-Q/TOF-MS studies.

Azelastine HCl is a second-generation H1 -receptor antagonist approved by the US Food and Drug Administration (US FDA) for treating seasonal allergic rhinitis and non-allergic vasomotor rhinitis. This study encompasses the validation of a liquid chromatography-ultra violet photo diode array (LC-UV/PDA) method for the drug and its extension to liquid chromatography/quadrupole time-of-flightmassspectrometry (LC-Q/TOF-MS) studies for identification and characterization of various stress degradation products of the drug. Stress degradation of azelastine HCl was undertaken under the International Council for Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) prescribed conditions of hydrolytic, photolytic, oxidative, and thermal stress. The degraded drug solutions were analyzed using Ultra Performance Liquid Chromatography (UPLC) employing a C18 (100 × 4.6mm; 2.6μ, Kinetex) column by isocratic elution. Detection wavelength was 241 nm. The degradation products were identified and characterized using UPLC-MS/TOF studies, and an attempt was made to isolate one of the degradation products by solvent extraction. The drug was found to significantly degrade under acidic/alkaline/neutral photolytic, oxidative, and alkaline hydrolytic conditions. Six degradation products (I-VI) were identified through LC-Q/TOF-MS studies that were adequately resolved from the drug with the developed UPLC method. All degradation products (I-VI) were ionized in the total ion chromatogram (TIC) in the LC-MS studies, and these were identified and characterized, and the degradation pathway of the drug was postulated. One of the oxidation products isolated from the degraded drug solution was characterized through differential scanning calorimetry, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance spectral data. Six degradation products generated from stress degradation studies on azelastine HCl were adequately resolved through LC-UV/PDA studies followed by method validation. These were successfully identified and characterized through LC-Q/TOF-MS studies, and the degradation pathways for the generation of these products from the drug have been postulated.

Alcoholic beverages reduce oxidation during in vitro gastrointestinal digestion of meat and fish, but induce fatty acid ethyl ester formation

The effect of selected phenolic-containing beers (pale lager, barley wine, tripel), white wine, and red wine on the formation of lipid oxidation products (thiobarbituric acid reactive substances, 4-hydroxy-2-hexenal, 4-hydroxy-2-nonenal) and protein carbonylation during simulated gastrointestinal digestion of pork and salmon was investigated. Greater antioxidant effects of the beverages were observed during the digestion of salmon, whereas antioxidant effects were less pronounced or absent during the digestion of pork. Red wine contained the highest levels of phenolics, accompanied by the highest antioxidant effects among the beverages tested. Secondly, the simultaneous formation of potentially harmful fatty acid ethyl esters (FAEEs) was observed for the first time when these muscle foods were digested with the beers and wines tested, or with pure ethanol. These FAEEs were formed by lipase activity in the presence of ethanol, which was blocked by the lipase inhibitor orlistat. The formation of selected FAEEs corresponded well with the levels of their precursor fatty acids in muscle, resulting in different FAEE profiles in the digests of chicken, pork, beef, or salmon with ethanol. In conclusion, the formation of potentially harmful FAEE may counteract the observed beneficial antioxidant effects of phenolic-rich alcoholic beverages during the gastrointestinal digestion of muscle foods.

Transcriptome Analysis Reveals the Mechanisms of Accumulation and Conversion of Folate Derivatives during Germination of Quinoa (Chenopodium quinoa Willd.) Seeds.

Folate was enriched during quinoa germination, while molecular mechanisms were not well understood. In this study, three quinoa varieties were selected for germination, and changes in substrate content and enzyme activity of the folate biosynthesis pathway were monitored. 5-Methyltetrahydrofolate (5-CH3-THF) and 5-formyltetrahydrofolate (5-CHO-THF) were significantly enriched in quinoa sprouts. Among the selected varieties, QL-2 exhibited the lowest content of the oxidation product MeFox and the highest total folate content. Based on transcriptome analysis, the p-ABA branch was found to be crucial for folate accumulation, while the pterin branch served as a key control point for the one carbon pool by folate pathway, which limited further folate biosynthesis. In the one carbon pool by folate pathway, genes CqMTHFR and CqAMT significantly contributed to the enrichment of 5-CH3-THF and 5-CHO-THF. Findings gained here would facilitate the potential application of quinoa sprouts as an alternative strategy for folate supplementation.

Regulation of P-Glycoprotein during Oxidative Stress.

P-glycoprotein (Pgp, ABCB1, MDR1) is an efflux transporter protein that removes molecules from the cells (outflow) into the extracellular space. Pgp plays an important role in pharmacokinetics, ensuring the absorption, distribution, and excretion of drugs and its substrates, as well as in the transport of endogenous molecules (steroid and thyroid hormones). It also contributes to tumor cell resistance to chemotherapy. In this review, we summarize the mechanisms of Pgp regulation during oxidative stress. The currently available data suggest that Pgp has a complex variety of regulatory mechanisms under oxidative stress, involving many transcription factors, the main ones being Nrf2 and Nf-kB. These factors often overlap, and some can be activated under certain conditions, such as the deposition of oxidation products, depending on the severity of oxidative stress. In most cases, the expression of Pgp increases due to increased transcription and translation, but under severe oxidative stress, it can also decrease due to the oxidation of amino acids in its molecule. At the same time, Pgp acts as a protector against oxidative stress, eliminating the causative factors and removing its by-products, as well as participating in signaling pathways.

Analysis of the oxidation behavior of TiBN and optical properties of oxidation products

In this research, we conducted an investigation into the oxidation behavior of a novel titanium boron nitride (TiBN) powder, with titanium nitride (TiN) powder used as comparative reference. At an oxidation temperature of 600 °C, the predominant oxidation products of TiBN powders were composed mainly of rutile, with residual TiBN remaining. In contrast, the oxidation products of TiN powders at 700 °C comprised both anatase and rutile phases. The TiBN powder demonstrated a lower rutile transition temperature and enhanced antioxidant activity compared to TiN. As observed in HRTEM and HAADF-STEM images, amorphous regions are discernible between TiBN particles, and the Ti, N, and O elements in the oxidation products of TiBN demonstrate uniform distribution. The lattice constant of the rutile phase formed during the oxidation of TiBN significantly surpasses that observed in the oxidation of TiN, attributed to the solid solution of the boron (B) element. DSC curves unveil both endothermic and exothermic peaks within the temperature range of 500 °C–800 °C during the oxidation of TiBN powders, indicating the occurrence of B2O3 melting alongside TiBN oxidation. Weight loss beyond 1050 °C is attributed to the evaporation of B2O3. DSC and TG curves suggest that the oxidation behavior of TiBN powders closely resembles that of titanium diboride (TiB2) powders, yet their oxidation resistance surpasses that of TiB2 powders. The oxidation products resulting from TiBN oxidation exhibit a narrower band gap than P25 TiO2, promising higher stability and enhanced electrochemical performance. Our innovative approach in developing a unique TiO2 photoactive material system could pave the way for novel photocatalysis applications.

A New Ultra High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method to Monitor 1-Amino-4-Methyl-Piperazine and 1-Methyl-4-Nitroso-Piperazine in Rifampicin Hydrolysis: A Standard Addition Approach to Quantify Nitrosamine in Rifampicin

1-amino-4-methyl-piperazine (AMP) is both a starting material for the synthesis of rifampicin (RIF) and a degradation product of RIF hydrolysis. 1-methyl-4-nitroso-piperazine (MNP) is an oxidation product of AMP as well as a potentially genotoxic N-nitrosamine. The EMA and FDA have approved an ad interim limit of 5 ppm for MNP in RIF drug products. As in-house methods for the analysis of MNP in RIF use a wide range of conditions for mobile phases and sample diluents, we decided to investigate whether these conditions affect the formation of MNP and AMP. A UHPLC-MS/MS method was developed to simultaneously quantify AMP and MNP during RIF hydrolysis in buffered aqueous solutions at different pH levels. Analyses were performed in MRM mode; separations were carried out on an InfinityLab Poroshell HPH-C18 (100 mm, 2.1 mm i.d., particle size 1.9 μm). In aqueous RIF solutions, the content of AMP and MNP increases with time; at different pHs, the concentration of AMP increases much faster in acidic than in basic solutions; and the increase in MNP can be reduced by the addition of ascorbic acid. To avoid an overestimation of MNP, water should not be used as a diluent in RIF sample preparations. Methanol is a more suitable diluent than water. A standard addition method has been validated for the quantification of MNP in RIF drug substances.

Design and competitive growth of different spinels at 750 °C for the multi-component CuCoMnFe alloy coatings

The diversified MnCoCuFe coatings can enhance the thermal growth stability and sintering property of spinel, and the multicomponent spinel exhibits good high temperature conductivity. The oxidation products of seven composite alloy coatings at 750 °C, CoNi(Fe), CoMn(Fe), CoMnCu(Fe), MnCu(Fe), MnCuCo(Fe), CuCoMn(Fe) and CuCo(Fe) coatings, were discussed in details. Due to differences in the elements and their concentrations in the designed coatings, some coatings could produce spinel, while others did not. The designed CuCo(Fe) coating and CuCoMn(Fe) coating prepared by electrodeposition in this study with the concentration ratio approximately nCu:nCo:nMn≈1:1:1. The oxidation reaction equations for the CuCoMnFe coating and CuCoFe coating at 750 °C were discussed. CoFe2O4 spinel was preferentially formed in the CuCoFe coating and CuMn2O4 spinel was preferentially formed in the CuCoMnFe coating. Both coatings demonstrated the good high-temperature oxidation resistance and high-temperature electrical conductivity.

Research on the Erosion of Gun Barrel Based on Vented Vessel Tests

The vented vessel test, as an alternation of the firing practice, is implemented to reconstruct the deterioration process of the gun barrel suffering the thermochemical erosion by propellant combustion gas. High pressure of over 300 MPa with a duration exceeding 20 ms is applied on the rifled specimen. A phase transition layer about 136 μm is observed by scanning electron microscopy (SEM) from the cross section of the test specimen. Some residual oxidation products of the iron can be detected by energy dispersive x-ray spectroscopy (EDS) on parts of the inner surface. A comprehensive erosion analysis is conducted, which estimates that the mass loss of the specimen is mainly attributed to the periodical formation and peeling of the chemical reaction layer on the bore surface. The most severely eroded part is located on the corner of the land. On the basis of the examined erosion mechanisms, a proper thermochemical erosion model is employed. The mass dissipation and the geometry evolution under continuous firing are simulated using finite element method (FEM). The simulation phenomenon shows good consistency with results of the experimental tests.

Confectionery products stored in permafrost conditions

Industrial development of the Arctic territories is a crucial direction in the development of our country. Expanding the market for confectionery products requires increasing their safety in various climatic conditions, which is only possible by ensuring quality indicators that guarantee their specified shelf life. Studies of confectionery products stored in conditions of unregulated negative temperatures of permafrost, according to organoleptic, physico-chemical and microbiological quality indicators, have been conducted. “Iris Kiss-Kiss” candies, “Strawberry” cookies, homemade crackers, biscuits, and chocolate were stored at unregulated low temperatures. In cookies, crackers, and biscuits, a foreign odor and rancid taste caused by oxidation products of the fat fraction were detected. As for chocolate after storage, heavy bloom and significant changes in structure and consistency were revealed. The moisture content of the chocolate increased. Storage conditions at low temperatures and high humidity increase the risk of microorganism development and microbiological spoilage. The results obtained allow concluding that long-term storage of confectionery products is possible under unregulated low temperatures.

Cleavage of the Disulfide Bonds via Hydrogenolysis for Recovering Iso‐octyl Thioglycolate from Distillation Residue in Iso‐octyl Thioglycolate Production

AbstractAbout 10–15 wt% of distillation residue, which is mainly composed of the oxidation products of iso‐octyl thioglycolate, harmful to the environment and difficult to dispose, is generated during refining through distillation. Herein we report an effective approach to recover iso‐octyl thioglycolate from the distillation residue by hydrogenolysis of the disulphide bonds. The effects of reaction temperature, reaction time, and feeding speed were examined. It is found that the rate of hydrogen formation is a key factor affecting the yield of iso‐octyl thioglycolate. The orthogonal test shows that the influence order is: reaction time > mass ratio of distillation residue/HCl > the molar ratio of HCl/Zn > reaction temperature. About 63.64 % iso‐octyl thioglycolate could be harvested after hydrogenolysis at 30 °C for 3.0 h. The mechanism study shows that iso‐octyl thioglycolate was regenerated by cleavage the disulfide bonds in iso‐octyl thioglycolate disulfides. Therefore, an alternative way for resource utilization of iso‐octyl thioglycolate distillation residue was developed.

Ammonium-Enhanced Arsenic Mobilization from Aquifer Sediments.

Ammonium-related pathways are important for groundwater arsenic (As) enrichment, especially via microbial Fe(III) reduction coupled with anaerobic ammonium oxidation; however, the key pathways (and microorganisms) underpinning ammonium-induced Fe(III) reduction and their contributions to As mobilization in groundwater are still unknown. To address this gap, aquifer sediments hosting high As groundwater from the western Hetao Basin were incubated with 15N-labeled ammonium and external organic carbon sources (including glucose, lactate, and lactate/acetate). Decreases in ammonium concentrations were positively correlated with increases in the total produced Fe(II) (Fe(II)tot) and released As. The molar ratios of Fe(II)tot to oxidized ammonium ranged from 3.1 to 3.7 for all incubations, and the δ15N values of N2 from the headspace increased in 15N-labeled ammonium-treated series, suggesting N2 as the key end product of ammonium oxidation. The addition of ammonium increased the As release by 16.1% to 49.6%, which was more pronounced when copresented with organic electron donors. Genome-resolved metagenomic analyses (326 good-quality MAGs) suggested that ammonium-induced Fe(III) reduction in this system required syntrophic metabolic interactions between bacterial Fe(III) reduction and archaeal ammonium oxidation. The current results highlight the significance of syntrophic ammonium-stimulated Fe(III) reduction in driving As mobilization, which is underestimated in high As groundwater.

Electron-Induced Damage by UV Photolysis of DNA Attached to Gold Nanoparticles.

Photolysis of DNA attached to gold nanoparticles (AuNPs) with ultraviolet (UV) photons induces DNA damage. The release of nucleobases (Cyt, Gua, Ade, and Thy) from DNA was the major reaction (99%) with an approximately equal release of pyrimidines and purines. This reaction contributes to the formation of abasic sites in DNA. In addition, liquid chromatography-mass spectrometry/MS (LC-MS/MS) analysis revealed the formation of reduction products of pyrimidines (5,6-dihydrothymidine and 5,6-dihydro-2'-deoxyuridine) and eight 2',3'- and 2',5'-dideoxynucleosides. In contrast, there was no evidence of the formation of 5-hydroxymethyluracil and 8-oxo-7,8-dihydroguanine, which are common oxidation products of thymine and guanine, respectively. Using appropriate filters, the main photochemical reactions were found to involve photoelectrons ejected from AuNPs by UV photons. The contribution of "hot" conduction band electrons with energies below the photoemission threshold was minor. The mechanism for the release of free nucleobases by photoelectrons is proposed to take place by the initial formation of transient molecular anions of the nucleobases, followed by dissociative electron attachment at the C1'-N glycosidic bond connecting the nucleobase to the sugar-phosphate backbone. This mechanism is consistent with the reactivity of secondary electrons ejected by X-ray irradiation of AuNPs attached to DNA, as well as the reactions of various nucleic acid derivatives irradiated with monoenergetic very-low-energy electrons (∼2 eV). These studies should help us to understand the chemistry of nanoparticles that are exposed to UV light and that are used as scaffolds and catalysts in molecular biology, curative agents in photodynamic therapy, and components of sunscreens and cosmetics.

The Activation of GABAAR Alleviated Cerebral Ischemic Injury via the Suppression of Oxidative Stress, Autophagy, and Apoptosis Pathways.

Ischemic stroke is a devastating disease leading to neurologic impairment. Compounding the issue is the very limited array of available interventions. The activation of a γ-aminobutyric acid (GABA) type A receptor (GABAAR) has been reported to produce neuroprotective properties during cerebral ischemia, but its mechanism of action is not yet fully understood. Here, in a rat model of photochemically induced cerebral ischemia, we found that muscimol, a GABAAR agonist, modulated GABAergic signaling, ameliorated anxiety-like behaviors, and attenuated neuronal damage in rats suffering cerebral ischemia. Moreover, GABAAR activation improved brain antioxidant levels, reducing the accumulation of oxidative products, which was closely associated with the NO/NOS pathway. Notably, the inhibition of autophagy markedly relieved the neuronal insult caused by cerebral ischemia. We further established an oxygen-glucose deprivation (OGD)-induced PC12 cell injury model. Both in vivo and in vitro experiments demonstrated that GABAAR activation obviously suppressed autophagy by regulating the AMPK-mTOR pathway. Additionally, GABAAR activation inhibited apoptosis through inhibiting the Bax/Bcl-2 pathway. These data suggest that GABAAR activation exerts neuroprotective effects during cerebral ischemia through improving oxidative stress and inhibiting autophagy and apoptosis. Our findings indicate that GABAAR serves as a target for treating cerebral ischemia and highlight the GABAAR-mediated autophagy signaling pathway.

Easily scale 3D conductive gradient fiber membrane for contaminants removal and fouling mitigation under electrochemical assistance

An electrochemical membrane filtration system provides an innovative approach to enhance contaminant removal and mitigate membrane fouling. There is an urgent need to develop portable, versatile, and efficient electrochemical membranes for affordable wastewater treatment. Here, a 3D conductive gradient fiber membrane (CC/PVDF) with a gradient porous structure was prepared using a two-step phase inversion method. Methyl orange (MO) was utilized as model organic substance to investigate the electrochemical performance of the CC/PVDF membrane. At applied potentials of +2 V, +3 V, −2 V and −3 V, the removal efficiency of MO was 5.1, 5.3, 4.8, and 5.1 times higher than at 0 V. A dramatic flux loss of 35.02% occurred on the membrane without electrochemistry, interestingly, whereas the flux losses were only 23.59%–10.24% in the applied potential after 30 min of filtration, which were approximately 1.18, 1.28, 1.29 and 1.38 times as high as that without electrochemistry, respectively. The enhanced removal and anti-fouling performances of the membranes were attributed to the functions of electrochemical degradation, electrostatic repulsion, and electrically enhanced wettability. Electrochemical generation of Hydrogen peroxide, along with HO• radicals, was detected and direct electron transfer and HO• were proved to be the dominant oxidants responsible for MO degradation. The intermediate oxidation products were identified by mass spectrometry, and an electrochemical degradation pathway of MO was proposed based on bond-breaking oxidation, ring-opening reactions, and complete oxidation. All the findings emphasize that the ECMF system possesses superior efficiency and creative potential for water purification applications.

Monitoring the oxidative function of hydroxytyrosol and potential interactions with glutathione produced by human cells

Hydroxytyrosol (HT) has outstanding antioxidant properties, however, the lack of information regarding its potential interactions with biological systems prevents the direct application of HT in the field of medicine. In particular, the interaction of HT with antioxidant defense systems based on tripeptides containing thiols (glutathione, GSH) remains elusive and requires further investigation. This study investigated the electrochemical behavior of HT in Britton-Robinson buffer solution (B-R) with and without GSH using bulk electrolysis (BE) and cyclic voltammetry (CV). The reaction pathways of HT and oxidation products were identified by combining ultrahigh-performance liquid chromatography and mass spectrometry (UHPLC-MS). The findings of the study indicate that the phenolic ring of HT experiences a quasi-reversible oxidation process. This process is associated with the oxidation of the catechol moiety through the transfer of 2e−/2H+ in the presence of existing GSH. As a result of this oxidation, a quinone is produced. The quinone molecule can undergo reduction, leading to the regeneration of the HT molecule. Alternatively, it can undergo further modification by interacting with GSH, resulting in the formation of mono-GSH-HT, bi-GSH-HT, and bi-GSH-HT trimer conjugates. Using density functional theory (DFT), the ratio of disulfide, mono-GSH-HT, bi-GSH-HT, and bi-GSH-HT trimer formation energies (Ef) was determined to be 4.5:1.8:1.1:1.4 which are consistent with UHPLC-MS measurement. Accordingly, the formation of the disulfide form of GSH and parent HT is preferred over the formation of other conjugates. The higher values of electrophilicity index (ω) for the HT trimer and quinone suggest a remarkable ability to interact with cells in biological systems.

Extreme weather exacerbates ozone pollution in the Pearl River Delta, China: role of natural processes

Abstract. Ozone (O3) pollution research and management in China have mainly focused on anthropogenic emissions, while the importance of natural processes is often overlooked. With the increasing frequency of extreme weather events, the role of natural processes in exacerbating O3 pollution is gaining attention. In September 2022, the Pearl River Delta (PRD) in southern China experienced an extended period (25 d) of regional O3 exceedances and high temperatures (second highest over last 2 decades) due to extreme weather conditions influenced by the subtropical high and typhoon peripheries. Employing an integrated approach involving field measurements, machine learning and numerical model simulations, we investigated the impact of weather-induced natural processes on O3 pollution by considering meteorological factors, natural emissions, chemistry pathways and atmospheric transport. The hot weather intensified the emission of biogenic volatile organic compounds (BVOCs) by ∼10 %. Isoprene and biogenic formaldehyde accounted for 47 % of the in situ O3 production, underscoring the predominant role of BVOC emissions in natural processes. The chemical pathway of isoprene contributing to O3 formation was further explored, with O3 production more attributable to the further degradation of early generation isoprene oxidation products (contributed 64.5 %) than the direct isoprene oxidation itself (contributed 35.5 %). Besides, it was found that the hot weather significantly promoted regional photochemical reactions, with meteorological factors contributing to an additional 10.8 ppb of O3 levels compared to normal conditions. Temperature was identified as the dominant meteorological factor. Furthermore, the typhoon nearing landfall significantly enhanced the cross-regional transport of O3 from northern to southern China through stratosphere–troposphere exchange (STE). The CAM-Chem model simulations revealed that the STE-induced O3 on the PRD surface could reach a maximum of ∼8 ppb, highlighting the non-negligible impact of STE. This study highlights the importance of natural processes exacerbated by extreme weather events in O3 pollution and provides valuable insights into O3 pollution control under global warming.

Gas-to-Particle Partitioning of Products from Ozonolysis of Δ3-Carene and the Effect of Temperature and Relative Humidity.

Formation of oxidized products from Δ3-carene (C10H16) ozonolysis and their gas-to-particle partitioning at three temperatures (0, 10, and 20 °C) under dry conditions (<2% RH) and also at 10 °C under humid (78% RH) conditions were studied using a time-of-flight chemical ionization mass spectrometer (ToF-CIMS) combined with a filter inlet for gases and aerosols (FIGAERO). The Δ3-carene ozonolysis products detected by the FIGAERO-ToF-CIMS were dominated by semivolatile organic compounds (SVOCs). The main effect of increasing temperature or RH on the product distribution was an increase in fragmentation of monomer compounds (from C10 to C7 compounds), potentially via alkoxy scission losing a C3 group. The equilibrium partitioning coefficient estimated according to equilibrium partitioning theory shows that the measured SVOC products distribute more into the SOA phase as the temperature decreases from 20 to 10 and 0 °C and for most products as the RH increases from <2 to 78%. The temperature dependency of the saturation vapor pressure (above an assumed liquid state), derived from the partitioning method, also allows for a direct way to obtain enthalpy of vaporization for the detected species without accessibility of authentic standards of the pure substances. This method can provide physical properties, beneficial for, e.g., atmospheric modeling, of complex multifunctional oxidation products.

Direct Photoreforming of Real-World Polylactic Acid Plastics into Highly Selective Value-Added Pyruvic Acid under Visible Light.

Although polylactic acid (PLA) represents a pivotal biodegradable polymer, its biodegradability has inadvertently overshadowed the development of effective recycling techniques, leading to the potential wastage of carbon resources. The photoreforming-recycling approach for PLA exhibits significant potential in terms of concepts and methods. However, the reaction faces enormous challenges due to the limited selectivity of organic oxidation products as well as the increased costs and challenging separation of organic products associated with alkali-solution-assisted prehydrolysis. Herein, we report an alkali-free direct-photoreforming pathway for real-world PLA plastics utilizing the Pd-CdS photocatalyst under visible-light illumination, obviating the need for chemical pretreatment of PLA. The devised pathway successfully produces H2 at a rate of 49.8 μmol gcat.-1 h-1, sustained over 100 h, and exhibits remarkable selectivity toward pyruvic acid (95.9% in liquid products). Additionally, experimental findings elucidate that Pd sites not only function as a typical cocatalyst for enhancing the photocatalytic evolution of H2 but also suppress competitive side reactions (e.g., lactic acid coupling or decarboxylation), consequently augmenting the yield and selectivity of pyruvic acid and H2. This investigation provides a straightforward and sustainable direct-photoreforming route capable of simultaneously mitigating and repurposing plastic waste into valuable chemicals, thus offering a promising solution to the current environmental challenges.

Oxidative fermentation of glucose and ethanol in designed media and cooked grape must by acetic acid bacteria

In this study, acetic acid bacteria strains were investigated for their ability to oxidize different carbon sources producing the corresponding oxidative products. Bacterial strains were cultivated in seven designed media and their acetification ability was assessed. The most performing strains were further tested to evaluate gluconic acid production in cooked grape must. Organic acids, sugars, and ethanol concentrations were assayed by high-pressure liquid chromatography. Overall, the findings showed high variability amongst strains of the same species, especially amongst Gluconobacter oxydans strains. However, strains ATCC 621H and DSM 3503T resulted to be the highest gluconic acid producers in all tested conditions. This study shows that grape must can be further valorized by selective fermentations for the production of gluconic based products.

Exerkines, Nutrition, and Systemic Metabolism.

The cornerstones of good health are exercise, proper food, and sound nutrition. Physical exercise should be a lifelong routine, supported by proper food selections to satisfy nutrient requirements based on energy needs, energy management, and variety to achieve optimal metabolism and physiology. The human body is sustained by intermediary and systemic metabolism integrating the physiologic processes for cells, tissues, organs, and systems. Recently, interest in specific metabolites, growth factors, cytokines, and hormones called exerkines has emerged to explain cooperation between nutrient supply organs and the brain during exercise. Exerkines consist of different compounds described as signaling moiety released during and after exercise. Examples of exerkines include oxylipin 12, 13 diHOME, lipid hormone adiponectin, growth factor BDNF, metabolite lactate, reactive oxygen species (ROS), including products of fatty acid oxidation, and cytokines such as interleukin-6. At this point, it is believed that exerkines are immediate, fast, and long-lasting factors resulting from exercise to support body energy needs with an emphasis on the brain. Although exerkines that are directly a product of macronutrient metabolism such as lactate, and result from catabolism is not surprising. Furthermore, other metabolites of macronutrient metabolism seem to be candidate exerkines. The exerkines originate from muscle, adipose, and liver and support brain metabolism, energy, and physiology. The purpose of this review is to integrate the actions of exerkines with respect to metabolism that occurs during exercise and propose other participating factors of exercise and brain physiology. The role of diet and macronutrients that influence metabolism and, consequently, the impact of exercise will be discussed. This review will also describe the evidence for PUFA, their metabolic and physiologic derivatives endocannabinoids, and oxylipins that validate them being exerkines. The intent is to present additional insights to better understand exerkines with respect to systemic metabolism.