Oxidized Carboxymethyl Cellulose Research Articles

Cellulose-based thermo-responsive hydrogel with NIR photothermal enhanced DOX released property for anti-tumor chemotherapy

Thermo-sensitive hydrogels change their properties through phase transition, which could be used to regulate various behaviors by changing the temperature. In this study, degradable hydrogels with thermo-response were designed by reaction of oxidized carboxymethyl cellulose (CMC-CHO) with functional P(NIPAM-co-AH). The hydrogels showed biocompatibility and thermo-response with lower critical solution temperature (LCST) regulated by weight ratio of P(NIPAM-co-AH)/CMC-CHO. The photo-thermal property of gold nanorod was triggered by the near-infrared (NIR) to enhance the DOX release for in vivo anti-tumor therapy of model mice. The results showed good biocompatibility and biodegradability of the hydrogel both in vitro and in vivo, and the DOX with hydrogel loading reduced the toxicity through sustained release behavior. The anti-tumor performance further enhanced with NIR triggered drug release regulated by photo-thermal property. In conclusion, the injectable P(NIPAM-co-AH)/CMC-CHO based self-healing hydrogels could act as promising drug delivery vehicle for potential localized anti-tumor therapy.

Fluorescent hydrogels based on oxidized carboxymethyl cellulose with excellent adsorption and sensing abilities for Ag+

Heavy metal contamination in water and soil are harmful and destructive to the environment, it has always been regarded as a big problem. Herein, we developed a self-healing fluorescent hydrogel based on oxidized carboxymethyl cellulose with excellent sensing and adsorption abilities for Ag+. The detection and adsorption effects of hydrogels on heavy metal ions were studied. It turned out that the fluorescent hydrogel has sensitive detection and high adsorption capacity for Ag+, the detection limit was 3.798 μM, and the maximum adsorption capacity was 407 mg/g. The adsorption isotherm fitted the Langmuir model well, and the pseudo-secondary model for adsorption kinetics fitted well. The hydrogel could heal itself without external stimulus, it could be easily regenerated 7 times without loss of adsorption performance. In short, the prepared hydrogel has capability of self-healing, detecting and adsorbing heavy metal ions at the same time, good mechanical strength, these all made it a promising long-life adsorbent and provided a new way for wastewater treatment.

Bioactive Injectable Hydrogel Dressings for Bacteria-Infected Diabetic Wound Healing: A "Pull-Push" Approach.

Chronic diabetic wound healing remains a challenge due to the existence of excessive danger molecules and bacteria in the inflammatory microenvironment. There is an urgent need for advanced wound dressings that target both inflammation and infection. Here, a bioactive hydrogel without loading any anti-inflammatory ingredients is rationally designed to achieve a "Pull-Push" approach for efficient and safe bacteria-infected diabetic wound healing by integrating danger molecule scavenging (Pull) with antibiotic delivery (Push) in the inflammatory microenvironment. The cationic hydrogel, termed the OCMC-Tob/PEI hydrogel, is fabricated by the conjugation of polyethylenimine (PEI) and tobramycin (Tob) on an oxidized carboxymethyl cellulose (OCMC) backbone via the Schiff base reaction with injectable, self-healing, and biocompatible properties. The OCMC-Tob/PEI hydrogel not only displays the remarkable capability of capturing multiple negatively charged danger molecules (e.g., cell-free DNA, lipopolysaccharides, and tumor necrosis factor-α) to ameliorate anti-inflammation effects but also achieves controllable long-term antibacterial activity by the pH-sensitive release of Tob. Consequently, this multifunctional hydrogel greatly expedites the wound closure rate with combined anti-inflammation and anti-infection effects on Pseudomonas aeruginosa-infected diabetic wounds. Our work provides a highly versatile treatment approach for chronic diabetic wounds and a promising dressing for regenerative medicine.

Preparation, Characterization, and Biological Evaluation of Transparent Thin Carboxymethyl-Chitosan/Oxidized Carboxymethyl Cellulose Films as New Wound Dressings.

Full thickness burns in which the damage penetrates deep into the skin layers and reaches underneath the muscle, compel the need for more effective cure. Herein, cross-linked carboxymethyl-chitosan (CM-chitosan) films, prepared by Schiff base association with oxidized carboxymethyl cellulose (OCMC), are investigated regarding the wound healing capacity on full thickness burn injuries in vivo. Transparent thin CM-chitosan/OCMC films are obtained with tensile strength reaching 6.11MPa, elongation at break above 27%, and water absorption more than 800%, which operates in favor of absorbing excess exudate and monitoring the wound status. Furthermore, the nonadherent CM-chitosan/OCMC films, with satisfactory biodegradability, cell, and tissue compatibility, are readily used to the wound sites and easily removed following therapy on scalded tissue so as to alleviate the suffering from burn. The films efficiently promote epithelial and dermal regeneration compared to the control, achieving 75.9% and 94.4% wound closure, respectively, after 14 and 27 days. More importantly, CM-chitosan/OCMC films accelerate wound healing with natural mechanisms which include controlling inflammatory response, reducing apoptosis, promoting fibroblast cell proliferation, and collagen formation. In conclusion, the CM-chitosan/OCMC films elevate the repair ratio of burn injuries and have great potential for facilitating the healing process on full-thickness exuding wounds.

A self-healing carboxymethyl chitosan/oxidized carboxymethyl cellulose hydrogel with fluorescent bioprobes for glucose detection

Self-healing hydrogel as a soft material with high durability and life-time has been successfully applied in various fields, including electronic skins, wearable electronic devices, and soft sensors. However, it is still a challenge to design a hydrogel with rapid self-healing, biodegradable and biosensing properties. Here, a self-healing carboxymethyl chitosan (CMCS)/oxidized carboxymethyl cellulose (OCMC) hydrogel with fluorescent bioprobes was developed for glucose detection. In this biosensing system, gold nanoclusters (AuNCs) and glucose oxidase (GOx) were encapsulated into the CMCS/OCMC hydrogel matrix as the fluorescent bioprobes. The CMCS/OCMC hydrogel with fluorescent bioprobes exhibited high sensitivity for glucose sensing with a linearly detection range of 100 μM to 5 mM and a detection limit of 0.029 mM, which covered the level of glucose in clinical detection. Furthermore, this hydrogel exhibited good biocompatibility. Finally, In vitro blood fluorescence tests and in vivo fluorescence investigation of the AuNCs-CMCS/OCMC hydrogel in diabetic mice indicated that this biocompatible and self-healing hydrogel based on fluorescent sensing system had potential application in implantable biosensing area for glucose monitoring.

Hydrogel Films Based on Chitosan and Oxidized Carboxymethylcellulose Optimized for the Controlled Release of Curcumin with Applications in Treating Dermatological Conditions.

Cross-linked chitosan (CS) films with aldehyde groups obtained by oxidation of carboxymethyl cellulose (CMC) with NaIO4 were prepared using different molar ratios between the CHO groups from oxidized carboxymethyl cellulose (CMCOx) and NH2 groups from CS (from 0.25:1 to 2:1). Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy demonstrated the aldehyde groups’ presence in the CMCOx. The maximum oxidation degree was 22.9%. In the hydrogel, the amino groups’ conversion index value increased when the -CHO/-NH2 molar ratio, cross-linking temperature, and time increased, while the swelling degree values decreased. The hydrogel films were characterized by scanning electron microscopy (SEM) and FTIR analysis. The curcumin encapsulation efficiency decreases from 56.74% to 16.88% when the cross-linking degree increases. The immobilized curcumin release efficiency (REf%) and skin membrane permeability were evaluated in vitro in two different pH solutions using a Franz diffusion cell, and it was found to decrease when the molar ratio -CH=O/NH2 increases. The curcumin REf% in the receptor compartment was higher at pH = 7.4 (18%- for the sample with a molar ratio of 0.25:1) than at pH = 5.5 (16.5%). The curcumin absorption in the skin membrane at pH = 5.5 (47%) was more intense than at pH = 7.4 (8.6%). The curcumin-loaded films’ antioxidant activity was improved due to the CS presence.

Antimicrobial/Biocompatible Hydrogels Dual-Reinforced by Cellulose as Ultrastretchable and Rapid Self-Healing Wound Dressing.

Hydrogels as a wound dressing, integrated with ultrastretchability, rapid self-healing, and excellent antimicrobial activity, are in high demand, particularly for joint skin wound healing. Herein, a multifunctional and ductile composite hydrogel was developed using poly(vinyl alcohol) (PVA)-borax gel as a matrix that was synergized or dual-reinforced with dopamine-grafted oxidized carboxymethyl cellulose (OCMC-DA) and cellulose nanofibers (CNF). Moreover, neomycin (NEO), an aminoglycoside antibiotic with multifunctional groups, was incorporated into the hydrogel network as both an antibacterial agent and a cross-linker. The dynamic reversible borate ester linkages and hydrogen bonds between OCMC-DA, PVA, and CNF, along with dynamic cross-linking imine linkages between NEO and OCMC-DA, endowed the hydrogel with excellent self-healing ability and stretchability (3300%). The as-reinforced networks enhanced the mechanical properties of hydrogels significantly. More remarkably, the composite hydrogel with improved biodegradability and biocompatibility is pH-responsive and effective against a broad spectrum of bacteria, which is attributed to the controllable release of NEO for steady availability of the antibiotic on the wound location. Overall, the antimicrobial hydrogel with rapid self-healing and reliable mechanical properties holds significant promise as dressing material for wound healing.

Improved antifouling performance of a polyamide composite reverse osmosis membrane by surface grafting of dialdehyde carboxymethyl cellulose (DACMC)

Antifouling properties are considered to be essential to polyamide (PA) composite reverse osmosis (RO) membranes for their industrial applications. In this work, an antifouling material, dialdehyde carboxymethyl cellulose (DACMC), was prepared by the oxidation of carboxymethyl cellulose (CMC) and then grafted onto the surface of a nascent PA RO membrane to improve its antifouling properties. The results showed that DACMC grafting could endow the membrane with improved hydrophilicity, surface smoothness and NaCl retention, while the permeability was decreased slightly after DACMC grafting. The zeta potentials of the membrane could be adjusted by changing the DACMC concentration. In addition, three typical surfactants with various charge properties, including sodium dodecyl sulfate (SDS), polyoxyethylene octylphenol ether (OP-10) and cetyltrimethylammonium bromide (CTAB), were used as feed foulants to evaluate the antifouling property of membranes. The results showed that, for the anionic SDS and nonionic OP-10 surfactants, the modified membranes with more DACMC had better antifouling performance because the membrane hydrophilicity dominated the interaction between foulants and the membrane surface. For the cationic CTAB, however, the pristine membrane showed better antifouling performance than the modified membrane with low DACMC grafting concentrations (5 and 10 g/L). This difference was attributed to the stronger electrostatic adsorption between the cationic CTAB and the modified membranes with more negative charges. The modified membrane with high DACMC grafting concentrations (20 g/L) exhibited the best antifouling performance for all the surfactants thanks to its high hydrophilicity and low negative charge. Therefore, the charge and hydrophilic properties of the RO membrane must be designed rationally to enhance the antifouling performance of the RO membrane when treating surfactant-containing wastewater.

3D printable and injectable lactoferrin-loaded carboxymethyl cellulose-glycol chitosan hydrogels for tissue engineering applications.

In this study, carboxymethyl cellulose (CMC)-glycol chitosan (GC) hydrogel, a potential three-dimensional (3D) printing biomaterial ink for tissue engineering applications was synthesized using simple, biocompatible in situ-gelling Schiff's base reaction and ionic interactions. Different grades of hydrogels (C70G30, C50G50 and C30G70) were synthesized at physiological conditions. The oxidation of CMC and imine bond formation in the hydrogel were confirmed spectroscopically. Scanning electron microscopic images revealed the crosslinked interconnected pores in the cross-sectioned hydrogels (dried). Swelling (equilibrium: 1 h), porosity (~75%), in vitro degradation (>30 days) and thermal gravimetric analyses of the dried gels were studied. Initially, cytotoxicity assay was evaluated using mouse osteoblastic cells (MC3T3). These experiments revealed that CMC-GC gels formed stable hydrogel networks and were biocompatible. Particularly, C50G50 gels showed high printability (continuous extrusion) and post-printing stability (without secondary crosslinking). Gel 3D printing was optimized by varying the air pressure, temperature, needle size and nozzle speed, to obtain stable lattice structures (2 to 16 layers). The printed (2 and 5 layers) hydrogels showed high stability in phosphate buffer saline (PBS) solution (1 h), under UV light (1 h) and after autoclaving. The strut dimensions and porosity of the printed gels before and after the stability tests were analyzed. The hydrogel stability may be attributed to both the imine bond and ionic interaction between the cationic and anionic polymer side chains. Lactoferrin (glycoprotein) incorporated C50G50 gels showed sustained release up to 21 days in PBS (pH 7.4) solution and demonstrated increased biocompatibility (>80%) during in vitro cytotoxicity assays (MC3T3 cells and bone marrow mesenchymal stem cells) and Live/Dead assay (MC3T3 cells). A higher number of live osteoblast cells on the C50G50 hydrogels with increasing lactoferrin concentration was observed. These results show that the CMC-GC gels are promising bio-ink candidates for 3D printing and loading proteins or drugs for tissue engineering applications.

Preparation of xylan bio-composite films reinforced with oxidized carboxymethyl cellulose and nanocellulose

Despite the vast availability and potential for use in biomaterials, application of xylan is highly restricted due to their hygroscopic nature and low mechanical strength. In this work, the xylan (Xy) from hot water extract of bagasse was successfully valorized by integration with carboxymethyl cellulose (CMC), oxidized carboxymethyl cellulose (OCMC) and cellulose nanofibers (CNFs). This work was conducted to blend different ratios of xylan, CMC, OCMC and CNF to provide compact bio-composite films with multi-functional characteristics. Xylan was isolated by using hot water treatment from bagasse, and OCMC was provided from selective oxidation of CMC with sodium periodate. The FTIR and XRD results confirmed the introduction of hydrogen bonding and electrostatic interaction in prepared bio-composite films. The tensile strength of the blended films improved by incorporation of the Xy-CMC with OCMC and CNF, and the WVP of the bio-composite films decreased. The maximum value of achieved tensile strength was 30 and 32 MPa for Xy-CMC/OCMC (codes A1–A8) and Xy-CMC/CNF (codes B1–B8), respectively. The thermal stability of bio-composite films had been improved compared to the neat xylan films. The overall results advised that the blended films potentially could be used in the coating and packaging applications. Preparation of xylan bio-composite films reinforced with oxidized carboxymethyl cellulose and nanocellulose.

Covalent Chitosan‐Cellulose Hydrogels via Schiff‐Base Reaction Containing Macromolecular Microgels for pH‐Sensitive Drug Delivery and Wound Dressing

AbstractWound dressings with pH‐sensitive properties, stable mechanical performance, and antibacterial properties have great potential for clinical applications. Herein, drug‐loaded microgels via Schiff‐base reaction between carboxymethyl chitosan and oxidized carboxymethyl cellulose are fabricated. Then they are embedded in hydrogels to form a hydrogel‐microgels composite (Gel/MGs). The gelation time, morphologies, swelling ratio, weight loss ratio, mechanical properties, pH‐sensitive drug release profiles, and antibacterial activities are examined. Adding microgels endows the hydrogels with more stable properties, enhanced mechanical performance, and drug release sensitivity to both acid and alkali in vitro. Gel/MGs dressings show a steady mass after 6 h and even maintain its 95.4% initial mass after 14 days in pH 7.4. In pH 9.5, Gel/MGs dressings also show a higher cumulative release of silver sulfadiazine than that in pH 7.4 and pH 5.5. Gel/MGs/AgSD dressings demonstrate desirable antibacterial properties, which may ensure their potential application in wound dressing.

Cellulose-based injectable hydrogel composite for pH-responsive and controllable drug delivery

Cellulose-based biocompatible, tunable and injectable hydrogels embedded with pH-responsive diblock copolymer micelles were constructed to achieve localized drug delivery with prolonged, stimuli-driven and slow-release function. First, we prepared two types of modified carboxymethyl cellulose (CMC) including hydrazide-modified carboxymethyl cellulose (CMC-NH2) and oxidized carboxymethyl cellulose (CMC−CHO) with varying degrees of oxidation. Then, pH-responsive poly (ethylene oxide)-block-poly (2-(diisopropylamino) ethyl methacrylate) (PEO-b-PDPA) copolymers as micelle cores to carry hydrophobic substances were also synthesized through atom transfer radical polymerization (ATRP). An injectable hydrogel composite system was finally obtained by mixing CMC-NH2 and CMC−CHO polymer suspensions containing PEO-b-PDPA copolymer micelles through a Schiff base reaction. This newly-synthesized, tunable, cellulose-based double barrier system exhibits a pH-triggered, prolonged, and slow-release profile based on the release test using both Nile Red dye and doxorubicin. The hydrogel system also exhibited comparable storage moduli and tunable degradation properties.

Development and characterization of bladder acellular matrix cross-linked by dialdehyde carboxymethyl cellulose for bladder tissue engineering.

In order to address the disadvantage of rapid degradation and serious immune response of bladder acellular matrix (BAM) tissues in clinical application, in this study, oxidized carboxymethyl cellulose (DCMC) was developed to replace glutaraldehyde (GA), a most common synthetic crosslinking reagent in clinical practice, to fix BAM tissues for lower cytotoxicity. The aim of this work was to evaluate feasibility of DCMC as a crosslinking reagent for BAM fixation and developing DCMC fixed-BAM (D-BAM) tissues for tissue engineering. For the preparation of DCMC, the results showed that when DCMC was prepared using a specific concentration of sodium periodate solution (the mass ratio of NaIO4/CMC is 1 : 1) and a specific reaction time (4 hours), its cytotoxicity was the lowest and its fixation effect was better. The critical crosslinking characteristics and cytocompatibility of optimum D-BAM tissues were also investigated. The results demonstrated that DCMC-fixation (especially 30 mg ml−1 DCMC-fixation) not only formed stable cross-linking bonds but also preserved well the original ultrastructure of the BAM tissues, which simultaneously increased the mechanical strength and capacity of the enzymatic hydrolytic resistance. The DCMC-fixation could also reduce the expression of α-Gal in BAM tissues and preserve the useful growth factors such as GAGs, KGF and TGF-β in bladder tissues. In addition, 30 mg ml−1 D-BAM tissues had excellent cytocompatibility. Moreover, it could stimulate the secretion of PDGF and EGF from seeded bladder transitional epithelial cells (BTECs), which is a critical feature for further re-epithelialization. Its anti-calcification ability was also prominent, which is necessary in bladder repair. The present studies demonstrated that DCMC could be a potential biological crosslinking agent for BAM fixation due to its excellent crosslinking effects, and the D-BAM tissues were suitable to be used as a substitute for the bladder due to their resistance to enzymatic degradation, anticalcification and cytocompatibility.

Gelatin — Oxidized carboxymethyl cellulose blend based tubular electrospun scaffold for vascular tissue engineering

The present work deals with the fabrication of electrospun tubular scaffold based on in-situ crosslinked blend of gelatin − oxidized carboxymethyl cellulose (OCMC) for vascular tissue engineering. The flow behavior and spinability of the hydrogel despite the in-situ crosslinked gelatin chains evaluated by Raman spectroscopic studies and rheological studies was utilized for electrospinning. The study highlights the tunable pore size and fiber diameter of the nanofibers with the manipulation of electrospinning parameters. With a future perspective of vascular tissue engineering, the electrospinning parameters yielding smooth bead free fibers and maximum magnitude in pore size and fiber diameter as well their homogenous distribution were selected for the fabrication of tubular constructs which is rarely reported. The surface and mechanical properties were evaluated to validate its properties to the native vessel. Biocompatibility was studied in vitro with BALB/c 3T3 cells and in vivo after subcutaneous implantation in rats. MTT assay confirmed its no-toxicity and no abnormal foreign body reaction were observed by 7 and 15days after implantation. Crosslinking with biocompatible crosslinker OCMC has rendered insolubility to gelatin yet making it spinable for electrospinning to fabricate porous, nanofibrous vascular biomaterial.

An Injectable Oxidized Carboxymethyl Cellulose/Polyacryloyl Hydrazide Hydrogel via Schiff Base Reaction

A series of injectable hydrogels was prepared by cross-linking oxidized carboxymethyl cellulose (oxi-CMC) with polyacryloyl hydrazide (PAH) via a Schiff base reaction under physiological conditions. The hydrogels exhibited superior performance such as appropriate rheology properties, high swelling ratio, and low degradation rate. In phosphate buffer solution (PBS, pH 7.4) at 37°C, the swelling ratio of the hydrogels ranged from 19 to 28 after 7 h, the degradation percentage of the oxi-CMC6/PAH3 hydrogel was ~47 % after 20 days. Using bovine serum albumin (BSA) as a model protein drug, the results of in vitro drug release studies demonstrated that the sustained release of BSA could be cooperatively controlled through drug diffusion and hydrogel degradation in PBS (pH 7.4) at 37°C, and the cumulative release percentage of BSA from a drug-loaded oxi-CMC6/PAH3 hydrogel was ~88 % after 8 days. The results signified that oxi-CMC6/PAH3 hydrogel could be potentially applied in the fields of drug delivery vehicles, tissue engineering, and cell encapsulation materials.

Grafting of oxidized carboxymethyl cellulose with hydrogen peroxide in presence of Cu(II) to chitosan and biological elucidation

The chemical interaction of chitosan (CS) is performed in the presence of sodium carboxymethyl cellulose (CMC) and/or oxidized CMC. The latter is obtained by the action of H2O2/CuSO4 to generate carbonyl and carboxyl groups which were increased with CuSO4 concentration. The characterization of these new materials is made by FTIR, TGA, XRD and SEM. Examination of the hemolytic potential showed that the hydrogels were non hemolytic in nature. The hydrogels were non-toxic and blood-compatible. The antibacterial and antioxidant activities of samples were investigated.

PH and redox dual stimuli-responsive injectable hydrogels based on carboxymethyl cellulose derivatives

pH and redox dual stimuli-responsive injectable hydrogels were prepared by cross-linking oxidized carboxymethyl cellulose (oxi-CMC) with 3,3′-dithiobis (propionohydrazide) (DTP) via Schiff base reaction under physiological condition. The hydrogels showed good performance such as tunable gelling time, appropriate rheology properties, high swelling ratio and low degradation rate. In vitro release studies confirmed that bovine serum albumin (BSA) as a model drug exhibited a sustainable release at pH 7.4 and an accelerated release under a lower pH 5.0 and/or reducing environments. The results signified that oxi-CMC/DTP hydrogels could be an attractive candidate for drug delivery system, tissue engineering or cell scaffold materials.

Understanding the in situ crosslinked gelatin hydrogel

AbstractThe present study focuses on an in‐depth investigation at the molecular level of in situ crosslinked gelatin hydrogel by oxidized carboxymethylcellulose (OCMC), intended for biomedical purposes. The crosslinking parameters, namely the crosslinking temperature, crosslinking time and the amount of crosslinker, were varied. The surface and bulk properties were investigated to construct a representative model postulating the interaction of gelatin and OCMC encompassing their physical and covalent linking which determines the properties of the hydrogel. It was inferred that covalent crosslinking facilitates the interfacial interaction between gelatin and OCMC which beyond 30% OCMC composition is impeded due to depletion: microdomain formation leading to phase separation. This study might enable the fabrication of tunable hydrogel with crosslinking parameters in accordance with its purpose. The preliminary biocompatibility studies of the hydrogel conclude that the crosslinked hydrogel generates favourable conditions for cells to adhere and proliferate showing potential for biomedical purposes. © 2015 Society of Chemical Industry

Change of Polysaccharide Molecular-Weight Distribution and Fraction Homogeneity After Periodate Oxidation

The effect of periodate oxidation on the molecular weight and fraction homogeneity of dextran dialdehydes and carboxymethylcellulose dialdehydes was studied using 13C NMR spectra and gel-permeation chromatography with dual detection. Oxidation of dextran formed homogeneous polymer fractions containing three types of oxidized residues. Oxidation of carboxymethylcellulose occurred more slowly, caused a sharp drop of molecular weight, and formed heterogeneous polymer fractions in the early reaction stages that became homogeneous at high conversions.

Preparation and Rheological Characterization of Cross-Linked Dialdehyde Carboxymethyl Cellulose

The carboxymethyl cellulose (CMC) is oxidized to the dialdehyde carboxymethyl cellulose (DACMC) at extremely acid media by sodium periodate. The reaction has been carried out at pH 2.0, temperature 35°C for 1.5h, with a NaIO4/CMC molar ratio of 1.1. FTIR peaks confirm the oxidation of CMC to DACMC. Furthermore, the rheological properties of CMC and DACMC were investigated by using steady shear and dynamic viscoelastic measurement in the range of concentrations (0.5~1 wt%). All of the CMC and DACMC solutions showed a shear thinning behavior over the shear rate at temperature from 30°C to 50°C. The zero shear viscosity (η0) was obtained by using the Cross model to fit experimental data. The η0values were used for detailed viscosity-concentration and activation energy analysis. The exponent in the viscosity-concentration power law was found to be lower than 1. The activation energy of the DACMC solution was bigger than CMCs. The effect of temperature on the storage modulus (G), the loss modulus (G) were also analyed in this study. In view of the foregoing aspects and our interest in the oxidation of carbohydrates by this oxidant, the present study is of great significant to gain some information on the cross-linked product of periodate oxidation, and is helpful for developing novel carboxymethyl polysaccharide derivatives.