Osteoplastic Material Research Articles

ASSESSMENT OF THE INTENSITY OF PAIN SYNDROME AND COLLATERAL SWELLING DURING THE SOCKET AUGMENTATION AFTER TOOTH EXTRAXTION WITH DIFFERENT OSTEOPLASTIC MATERIALS

The article presents a comparative analysis of the clinical effectiveness of osteoplastic materials, in particular a preparation based on multipotent mesenchymal stromal cells of adipose tissue and platelet-enriched plasma used to fill bone defects during the socket augmentation after the tooth extraction. The criteria to assess the therapy effectiveness at the stage of the early postoperative period included the intensity of pain and the amount of collateral oedema after surgery. The aim of this study is to evaluate the clinical effectiveness of osteoplastic materials to augment the volume of the alveolar bone during the operation of socket augmentation after the tooth extraction. Setting of this study: Bukovynian State Medical University, Chernivtsi, Ukraine. The study included 85 patients. The procedure of socket augmentation was performed by using "Kolapan - L" (28.57% of individuals, subgroup A), by combinations of MMSK AT + "Kolapan - L" + PRP (46.43% of individuals, subgroup B); the subgroup C included individuals (25.0%) whose wounds after tooth extraction healed by the formation a blood clot. The assessment of postoperative pain was performed by applying the digital rating scale (Numerical Rating Scale, NRS), taking into account the subjective pain in the patients. The subjects underwent visual assessment of the severity of collateral oedema after the tooth extraction surgery. To determine the dynamics of the severity of oedema we used a scoring system. The study demonstrated the patients of the subgroup B, who received the socket augmentation with "Kolapan - L" + MMSC-AT + PRP, experienced no pain at the final stage of postoperative observation (89.31% of cases) that was 1.2 times higher (p1 <0.05) than in the patients of subgroup A (p> 0, 05). At the final postoperative stages, no collateral oedema was seen in 98.47% of the participants of the subgroups B that exceed the number of cases in the subgroup A where the bone defect was augmented by "Kolapan - L", in 1.2 times (p1 <0.05).
 A comparative analysis of the clinical effectiveness of osteoplastic materials to increase the volume of the alveolar bone in the jaws during the socket augmentation after the extracted tooth convincingly proved the advantage of using the combination of "Kolapan - L" + MMSС - AT + PRP that was also confirmed by signs and symptoms in the postoperative period.

COMPARATIVE EVALUATION OF NEW OSTEOPLASTIC MATERIALS BASED ON THE RESULTS OF ACUTE TOXICITY STUDIES

Three series of experiments were carried out and lethal doses of the new osteoplastic material 47.5 V were determined by the intraperitoneal and intragastric injection of the material to laboratory animals. A comparative evaluation with Bioactive glass BG-1D was also carried out. It was found that the LD50of 47,5B was 4274.51:4770.58 mg/kg for intragastric injection and 2358.31:2895.65 mg/kg for intraperitoneal injection to rats. In animals getting Bioactive glass BG-1D, these indicators changed slightly, amounting to 3439.04:3810.53 mg/kg and 1732.77:2730.93 mg/kg, respectively. Thus, according to the classification of substances according to the degree of toxicity, these materials can be attributed to practically non-toxic substances (according to the results of intraperitoneal injection of the material suspension to rats and mice) and low-toxic substances (according to the results of intragastric injection of the material suspension to rats)

Комплексное лечение больных с хроническим генерализованным пародонтитом средней степени тяжести с использованием методов остеогингивопластики биокомпозиционными материалами и направленной тканевой регенерации

Improvement of methods of complex treatment of periodontium diseases continues to be an urgent problem of dentistry and requires an interdisciplinary approach. The severity of the course of periodontitis and the severity of destructive phenomena dictates the need to use osteoplastic agents to restore damaged periodontium tissues. It has been proven that the efficiency of membrane technology is significantly increased when membranes and substitute materials are used together. The positive effect of the combined use of Platelet Rich Plasma (PRP — autogenous growth factors), osteoplastic materials, and the method of Guided Tissue Regeneration (GTR) has been confirmed. To increase the efficiency of reconstructive treatment of patients with generalized periodontitis through the combined use of alloplasty, Platelet Rich Plasma, and the method of Guided Tissue Regeneration. Method of testing: clinical, X-ray. For the period 2018–2019, 34 patients with chronic generalized periodontal atrophy of moderate severity were under observation. All patients underwent professional oral hygiene, laser therapy, and antibacterial therapy with rovamycin. 6 patients in complex therapy underwent surgical interventions: osteogingivoplasty with biocomposite materials using GTR auto-allogenic membranes. Bone defects were filled with biocomposite material: hydroxylappatite granules (Polistom, RF) in combination with PRP, on top of an alto plasma membrane isolated from PRP and collagen membrane (Polistom, RF). The immediate and long-term periods of treatment were quite favorable. Control X-ray examination after 12 months showed restoration of a bone defect with an organotypic structure.

Хирургическое лечение обширных кист челюстей с использованием остеопластики биокомпозиционными материалами и метода направленной тканевой регенерации

Improvement of methods of treatment of odontogenic cysts of the jaws continues to be an urgent problem in surgical dentistry and maxillofacial surgery. Currently, the most promising is the use of multicomponent osteoplastic materials in order to optimize reparative osteogenesis after cystectomy for extensive jaw cysts. The aim of the study is to improve the method of cystectomy in the surgical treatment of large cysts of the upper jaw with osteoplasty of the bone defect with biocomposite materials in combination with the method of directed tissue regeneration. Clinical, radiological, histological. During the period 2015-2019, 54 patients with odontogenic cysts of the jaws were under observation, of which 12 were diagnosed as extensive. After cystectomy, the bone cavity was filled with biocomposite material: hydroxylappatite granules (Polistom, RF) in combination with PRP, on top of an autoplasma membrane isolated from PRP and collagen membrane. The immediate and long-term periods of treatment were quite favorable. Control X-ray examination after 12 months showed complete recovery of the bone defect with an organotypic structure in all patients.

Treatment of Femoral Non-Union with the Gene-Activated Osteoplastic Material: А Case Report

Background. Non-unions of distal femur fractures are difficult to treat and occur in about 6% of cases. Multifactorial causes of fractures non-unions require individual treatment for each patient in accordance with the “diamond” concept. The standard protocol for patients with atrophic non-unions treatment involves bone autografts using, but there are limitations of size, shape, quality and quantity of autografts. Osteoplastic materials with osteoinductive (angiogenic) and osteoconductive activity can be used as bioresorbable implants in combination with autogenous spongy bone in the treatment of extremities long bones non-unions.Clinical case description. A 63-year-old patient was admitted to the clinic for non-union of distal third of the femur with bone defect, fragments were fixed with a plate. The examination revealed plate fracture, screws migration (group III according to the Non-Union Scoring System). The volume of supposed bone defect was about 8.5 cm3. The surgery was performed: plate removal, debridement of the non-union zone, femur defect replacement with a bone autograft in combination with the gene-activated osteoplastic material “Histograft” in a ratio of 1:1, osteosynthesis of the femur with two plates. After 6 months. during the control computed tomography, consolidation was determined (4 points on the REBORNE scale). Pain was practically absent (NRS-2). The range of motion in the knee joint: flexion — 80o, extension — 180o. According to the Knee Society Score (KSS) — 68 points.Conclusion. In this case report the complete fracture fusion was achieved in patient within 6 months — 4 points on the REBORNE scale. No adverse events were observed. It confirms the safety and efficacy of described method and allows to continue the clinical trials.

Аддитивные технологии у пациентов с обширными дефектами костей нижних конечностей

Augments and reinforcing constructs made of titanium with a porous coating have been used for bone defect management in addition to osteoplastic materials. Additive technologies in traumatology and orthopedics for extensive defects in bones and joints have been applied when it is impossible to use standard designs. The purpose of the study was to evaluate short-term results and perspectives of using additive technologies for bone defects after failed joint arthroplasties and osteosynthesis. Materials and methods In 2018 to November 2019, seven patients with lower extremity bone defects underwent treatment at the Department of Traumatology and Orthopedics of the Moscow Regional Research and Clinical Institute with custom-made implants fabricated with additive technologies. The operations were carefully planned using CT scans, 3D modeling, and implant printing. Particular attention was paid to clean the implant from residual metal powder. Patients were distributed depending on the type of defect and the operation performed (arthroplasty, revision arthroplasty). Results The short-term results of using customized implants were analyzed in this study. The average Harris hip score before surgery was 37.8 points, and after the surgery it was 80.2 points. Pain after surgery in all patients was also evaluated by Harris scale and was 37.1 points; the functionality of patients after surgery was 38.4 points. The custom-made designs have a number of distinguishing advantages against the standard ones. A customized anatomical design provides easier fixation; ergonomic design allows implant retention without removing metal fixators if exist; there is less soft tissue injury during surgery (allinside principle); and convenient bone grafting around the implant. Conclusions The use of additive technologies for bone defects improves the functional results and life quality of the patient. At the moment, practical application of 3D designs has a number of limitations in financial and legal support in practical health care. Further implementation of additive technologies in traumatology and orthopedics will be supported by the grant from the President of the Russian Federation.

Dynamic contrast-enhanced magnetic resonance imaging for monitoring neovascularization during bone regeneration\u2014a randomized in vivo study in rabbits

ObjectivesMicro-computed tomography (μ-CT) and histology, the current gold standard methods for assessing the formation of new bone and blood vessels, are invasive and/or destructive. With that in mind, a more conservative tool, dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), was tested for its accuracy and reproducibility in monitoring neovascularization during bone regeneration. Additionally, the suitability of blood perfusion as a surrogate of the efficacy of osteoplastic materials was evaluated.Materials and methodsSixteen rabbits were used and equally divided into four groups, according to the time of euthanasia (2, 3, 4, and 6 weeks after surgery). The animals were submitted to two 8-mm craniotomies that were filled with blood or autogenous bone. Neovascularization was assessed in vivo through DCE-MRI, and bone regeneration, ex vivo, through μ-CT and histology.ResultsThe defects could be consistently identified, and their blood perfusion measured through DCE-MRI, there being statistically significant differences within the blood clot group between 3 and 6 weeks (p = 0.029), and between the former and autogenous bone at six weeks (p = 0.017). Nonetheless, no significant correlations between DCE-MRI findings on neovascularization and μ-CT (r =−0.101, 95% CI [−0.445; 0.268]) or histology (r = 0.305, 95% CI [−0.133; 0.644]) findings on bone regeneration were observed.ConclusionsThese results support the hypothesis that DCE-MRI can be used to monitor neovascularization but contradict the premise that it could predict bone regeneration as well.

Porous Polymer Scaffolds based on Cross-Linked Poly-EGDMA and PLA: Manufacture, Antibiotics Encapsulation, and In Vitro Study.

Porous polymer materials derived from poly(ethylene glycol dimethacrylate) (poly-EGDMA) and antibiotic containing polylactide (PLA) are obtained for the first time. Porous poly-EGDMA monoliths with a system of open interconnected pores are synthesized by a visible light-induced radical polymerization of EGDMA in the presence of 70wt% of porogenic agent, e.g., 1-butanol, 1-hexanol, 1-octanol, or cyclohexanol. The porosity of the obtained polymers is 75-78%. A modal pore size depends on the nature of the porogen and varies from 0.5µm (cyclohexanol) to 12µm (1-butanol). The polymer matrix made with 1-butanol features the presence of pores ranging from 1 to 100µm. The pore surface of poly-EGDMA matrices is inlayered with poly-D,L-lactide (Mn 23 × 103 Da, PDI1.31). The PLA-modified poly-EGDMA retains a porous structure that is similar to the initial poly-EGDMA but with improved strength characteristics. The presence of antibiotic containing PLA ensures a high and continuous antibacterial activity of the hybrid polymeric material for 7 days. The nontoxicity of all the porous matrices studied makes them promising for clinical tests as osteoplastic materials.

CURRENT TRENDS IN BONE AUGMENTATION DURING DENTAL IMPLANTATION IN PATIENTS WITH PARODONTAL PATHOLOGY AND JAW BONE ATROPHY (LITERATURE REVIEW)

Today, despite the progress made in dental implantology, the problem of rehabilitation of patients with significant bone atrophy in parodontal diseases has not been fully studied.
 The study aims to review the current views of various authors on the aetiology and treatment of parodontal diseases, which caused multiple tooth loss and bone atrophy in patients with occlusal pathology, based on the literature sources of scientific and medical information.
 Object and methods of study. Analytical study of scientific and medical literature on the treatment of multiple and partial tooth loss in patients with parodontal diseases in bone atrophy with impaired jaw relationships and orthopaedic rehabilitation using dental implantation and methods of bone augmentation of the mandible and maxilla.
 The use of implants in orthopaedic treatment expands the possibilities of using fixed prostheses, satisfying patients functionally and aesthetically. In case when the preservation of teeth becomes impossible or impractical to ensure proper treatment of the patient, they can be removed and replaced with dentures on dental implants.
 According to Misch C. [6], the masticatory load, which is exerted on the bone tissue of the jaws by an implant, increases the density of the bone structure. When teeth are lost due to parodontitis, there is atrophy of the alveolar ridge and a change in the structure of bone tissue, which is characterized by an increase in the porosity of the cancellous bone [4;5;7]. Sufficient bone volume and density in the area of the planned implantation are the main and important parameters for achieving normalized primary implant stabilization [11]. Partial and complete loss of teeth, as well as parodontal diseases, are always accompanied by the bone tissue of alveolar bone tissue of varying severity [1;4;5;13]. Signs of parodontal diseases in dentition defects are always more pronounced than in intact dental arch. The disease progresses rapidly, the dentition is destroyed if appropriate occlusal therapy is not performed [13;14]. Systemic factors and comorbidities in patients also play a significant role in reducing the bone volume of the jaws.
 The relationship between decreased bone density and age-related hormonal changes in women, as well as at the gender level, has been studied. Thus, women have changes in bone architecture and less dense arrangement of trabeculae in the jawbones than men [15].Bone tissue has a distinguishing feature – loss of multiple teeth or parodontal diseases lead to a redistribution of functional load, which causes its resorption [7;20]. In this regard, tooth extraction inevitably leads to a decrease in bone volume. The success of dental implantation on the maxilla became partially possible due to the introduction of sinus lifting with the use of osteoplastic materials [12;20;21]. According to some scientists[21], the autogenous bone graft is the gold standard in regenerative processes due to its osteoinductive, osteoconductive, and osteointegration properties, which are necessary for the restoration of lost bone tissue.
 The use of dentin in human teeth as an autograft was first described in a clinical case report in 2003 [23]. In recent experiments, these materials have proven to be a real alternative to the bone graft. Thanks to the work of a group of researchers [23;24], significant efforts were made in the basic and clinical study to find the best bone graft material for osseointegration of implants in the maxillary sinus. The autogenous dentin graft (AutoBT; Korea Tooth Bank, Seoul, Korea) was first developed in 2008, and several clinical studies have praised the use of AutoBT in the sinus compared to other grafts.
 Given the current development of advanced technologies in dental implantation, dentists use navigation templates in their practice, which are made using CAD CAM technology to improve the quality of care for patients who have indications for dental implantation. New diagnostic approaches allow us to virtually plan, simplify, and speed up surgery, to ensure accuracy. Thanks to this technology, dentists optimize the location of dental implants, taking into account the difficult conditions specific to patients with parodontal pathology.

New-generation osteoplastic materials based on biological and synthetic matrices

Objectives. The purpose of this analytical review is to evaluate the market for osteoplastic materials and surgical implants, as well as study the features of new-generation materials and the results of clinical applications.Methods. This review summarizes the volumes of research articles presented in the electronic database PubMed and eLIBRARY. A total of 129 scientific articles related to biological systems, calcium phosphate, polymer, and biocomposite matrices as carriers of pharmaceutical substances, primary recombinant protein osteoinductors, antibiotics, and biologically active chemical reagents were analyzed and summarized. The search depth was 10 years.Results. Demineralized bone matrix constitutes 26% of all types of osteoplastic matrices used globally in surgical osteology, which includes neurosurgery, traumatology and orthopedics, dentistry, and maxillofacial and pediatric surgery. Among the matrices, polymer and biocomposite matrices are outstanding. Special attention is paid to the possibility of immobilizing osteogenic factors and target pharmaceutical substances on the scaffold material to achieve controlled and prolonged release at the site of surgical implantation. Polymeric and biocomposite materials can retard the release of pharmaceutical substances at the implantation site, promoting a decrease in the toxicity and an improvement in the therapeutic effect. The use of composite scaffolds of different compositions in vivo results in high osteogenesis, promotes the initialization of biomineralization, and enables the tuning of the degradation rate of the material.Conclusions. Osteoplastic materials of various compositions in combination with drugs showed accelerated regeneration and mineralization of bone tissue in vivo, excluding systemic side reactions. Furthermore, although some materials have already been registered as commercial drugs, a plethora of unresolved problems remain. Due to the limited clinical studies of materials for use on humans, there is still an insufficient understanding of the toxicity of materials, time of their resorption, speed of drug delivery, and the possible long-term adverse effects of using implants of different compositions.

Study on tensile, bending, fatigue, and in vivo behavior of porous SHS–TiNi alloy used as a bone substitute

Intermetallic porous SHS–TiNi alloys exhibit tangled and specific stress–strain characteristics. This article aims to evaluate the findings emanating from experiments using standard and proprietary instruments. Fatigue testing under repeated complex loading was used to measure the total number of load cycles before failure of the SHS–TiNi samples occurred. Of the tested samples, seventy percent passed through 106 cycles without failure due to the reversible martensite transformation in the TiNi phase, one of the prevailing constituents of a multiphase matrix. The fractured surfaces were analyzed using scanning electron microscopy and confocal laser scanning instruments. Microscopy studies showed that the entire surface of the sample is concealed by miscellaneous strata that result from the SHS processand effectively protect the porous alloy in a corrosive environment. Numerous non-metallic inclusions, which are also attributed to the SHS reaction, do not have a significant impact on the deformation behavior and fatigue performance. In this context, the successful in vivo functioning of porous grafts assessed in a canine rib-plasty model allows the bone substitute to be congruentially deformed in the body without rejection or degradation; it thus has a long operational life, often greater than 17 ×106 (22 × 60 × 24 × 540) cycles. It acknowledges the potential benefits of SHS–TiNi as a superior osteoplastic material and its high resistance to corrosion fatigue.

Autologous regenerative stimulants for bone allograft implantation

There are many different surgical techniques for bone reconstruction. However, biological reconstruction methods are being increasingly developed. The main purpose is not only to fill up defects, but to stimulate the processes of reconstruction and regeneration of bone as a complete organ. In this report, we describe the basic principles of orthobiology and the essential orthobiological materials. A clinical case is presented where a combination of allogeneic osteoplastic materials with autologous platelet-rich plasma is used to reconstruct a cavity defect in the tibia.

Comparative Characteristics of Bone-Plastic Materials for Mastoidoplasty

The paper presents the results of the comparative characteristics of the use of various bone-plastic materials for mastoidoplasty. The study included 30 patients who underwent an open-type sanitizing operation with mastoidoplasty with osteoplastic materials. When comparing drugs, the following criteria were used: 1) convenience of modeling the material; 2) the course of the wound process; 3) osseointegration - the process of formation of "new bone tissue (CT of the temporal bones 6 and 12 months). As a result, it was confirmed that the samples of the main groups of bone replacements studied by us. preparations (biositalls, composite materials and β- tricalcium phosphates) can be successfully used in mastoidoplasty after open types of sanitizing operations on the middle ear.

Реконструкція дефектів коміркового відростку як запорука вдалого ортопедичного лікування

Resume. The aim of the work was to study the peculiarities of the choice of the newest methods of reconstruction of bone and tissue defects of the cellular process of the jaw. With Smart Dentin Grinder, you can easily turn a removed tooth into a dentin autograph ready for transplantation in just a few short steps. That will provide a sparing fence, full biocompatibility, high ostogenerating potential of autoplastic material, which can serve as removed teeth, which become grafts. Key words: osteoplastic material, bone defect, technique.

Экспериментальная модель хронического очагового остеомиелита для исследований in vivo

Introduction. The article describes an experimental model of chronic suppurative osteomyelitis in a rabbit. As new therapeutic and diagnostic algorithms for the supervision of patients with osteomyelitis appear, there is an growing need to compare the methods of surgical debridement and plasty of bone defects in an infectious process, in order to create experimental standardized pathological conditions as close to the clinical course of the disease in humans as possible. The aim of the study was to develop an experimental model of a standardized chronic purulent bone cavity, suitable for a comprehensive assessment of surgical debridement effectiveness and osteoplastic properties of bone substitute materials. Materials and methods. A standardized defect of the tibia in 24 rabbits was formed. The Staphylococcusaureus strain was used as an infectious agent. A dynamic assessment of the main indicators of blood counts in animals was carried out. The formation of chronic osteomyelitis was evaluated using radiography, com-puted tomography methods and histological studies. Results. It was shown that purulent bone wound developed in experimental animals with the technique cre-ated, and a defect with signs of a chronic purulent-inflammatory process was demonstrated. Conclusion. The proposed model of chronic osteomyelitis is reproducible. Operational flexibility and identi-cal in size and location bone defects allow to use this model in new osteoplastic material research. Keywords: chronic osteomyelitis, experiment, experimental animals

Evaluation of 3D Printed Computer Assisted Patient’s Specific Scaffold Seeded by Autologous Bone Marrow Mononuclear Cells in Mandibular Reconstruction

Purpose: This study was carried out to evaluate both clinically and radiographicly the use of 3D printed computer assisted patient’s specific scaffold seeded with ABMMCs in mandibular reconstruction. Materials and methods: The present study was conducted on eight patients who needed mandibular reconstruction. All patients in this study were subjected to mandibular reconstruction using 3D printed computer assisted patients specific scaffold using PLLA scaffold seeded with ABMMCs combined with osteoplastic materials of biological origin as Osteomatrix (LLC Konectbiopharm, Russia) which composed of natural collagen and hydroxyapatite with affinity-bound sulfated glycosaminoglycan (SGAG). All patients underwent preoperative cranial-maxillofacial CT scans, immediate postoperative and after six months for assessment of bone density and volume changes. Results: The reduction in bone density (17.4%) and bone volume (14.5%) from immediate to six months was statistically significant. There was an increase in bone density in the area near the autogenous bone of the patient than the area far from the autogenous bone of the patient. Conclusion: Using 3D printed computer assisted patient’s specific scaffold seeded with ABMMCs combined with Osteoplastic materials of biological origin in mandibular reconstruction is useful in small defects.

IMMEDIATE IMPLANTATION AND AESTHETIC COMPONENT AS A RESULT OF SUCCESSFUL FORECAST TREATMENT

The aim: Improving the method of immediate implantation in the aesthetic zone in case of bone deficiency to obtain the highest aesthetic and predictable treatment result. Materials and methods: Under clinical observation were 32 patients with different clinical diagnoses in the anterior part of the upper jaw aged 30 to 55 years. In the course of recent advances, the following methods have been used: clinical protocol of immediate implantation with passive exceptional loads by temporary orthopedic constructions, X-ray method using cone-beam computed tomography, statistical analysis. Results: After surgical treatment of patients 1 year after surgery, the distribution of biotypes was as follows: in group 1 – thick biotype 12.87%, medium – 87.13%; in group 2 – thick biotype 27.04%, medium – 72.96%, with p <0.05. According to the results of CT, the distance between the implant and the vestibular in the first group was after 6 months – 1.67 ± 0.04 mm (p <0.05); in the second group of the study we obtained the following results after 6 months – 1.59 ± 0.06 mm (p <0.05). Conclusions: The advanced method of immediate implantation in the anterior part of the upper jaw allows to change the biotype of soft tissues, improve the color spectrum of the gums, increase the thickness of soft tissues with connective tissue autograft, and increase gum density and fixation of osteoplastic material in the presence of defect ), as well as reduce the risk of recession.

The anti-inflammatory and osteoinductive effect of Simvastatin, possibilities of its use for treatment of periodontal diseases

The successful use of surgical and medical methods for the treatment of jawbone restoration has been convincingly confirmed in clinical practice. At the same time, technologies for potentiating the osteoinductive properties of osteoplastic materials to restore bone volume continue to develop. The inclusion of statins in the composition of osteoplastic materials alone or in combination with MSCs is one of the new and promising areas of regenerative action. Objectives — collection and analysis of scientific data on the influence of SMV on the differentiation of MSCs, the participation of SMV in inflammatory processes in periodontal tissues, the search for prospects for the use of SMV as a component of complex therapy of periodontal diseases. Materials and methods. The research of data has been carried out on the databases: PubMed.com, ScienceDirect.com, eLibrary.ru, by the following key words: “simvastatin” AND “periodontitis”, “simvastatin” AND “stromal mesenchymal cells” over the time period from 2014 to 2020; the articles have been selected on the basis of a number of experimental works. Results. The main cellular and genetic ways of implementing the work of SMV in relation to MSCs and periodontal diseases were emphasized, the correlation between the dose-dependent use of SMV with the differentiation of MSCs and the severity of the inflammatory response in periodontal diseases was revealed. Conclusion. SMV is a promising tool for periodontal diseases both at the inflammatory and rehabilitation stages.

Experimental study of the bearing capacity of lyophilized allogeneic bone material

Was to conduct an experimental study of the bearing capacity and determination of the Young's modulus of lyophilized allogeneic bone material. The study used 5 prototypes of lyophilized allogeneic bone blocks, which were alternately subjected to static loading by compressive force. The experimental values of Young's modulus of all bone samples were determined. When applying the Student's probability distribution in statistical processing of experimental data, the average indicators of the forces of destruction of the samples were obtained and Young's modulus was determined. As a result of the study, the indices of the maximum loading (6012H) and Young's modulus (147.2±0.228 MPa) of lyophilized allogeneic bone material were obtained. The obtained Young's modulus of lyophilized allogeneic bone material is of great importance in scientific activity, providing the possibility of further investigation of the properties of lyophilized allogeneic bone tissue to form a preoperative forecast of the behavior of an osteoplastic material and a dental implant installed in it.

Obliteration of the middle ear cavity in children: the current state of the problem and our first experience

In the period from May 2018 to November 2020, on the basis of the Federal State Autonomous Institution «National Medical Research Center of Children's Health» of the Ministry of Health of Russia, 28 children aged from 6 to 17-year-old with chronic otitis media and cholesteatoma were operated using osteoplastic obliteration, who had previously sanitizing operation on the middle ear. All children on admission to the hospital and 6-12 months after the operation underwent a complex examination. The condition of each patient was assessed using the OMO-22 quality of life questionnaire, to which the parents answered the questions twice - before and 1 year after surgery. During the postoperative period none of the patients had clinical signs of recurrence of cholesteatoma, which was confirmed by CT scan. When assessing the quality of life of children using a questionnaire, the average score before the operation was 130.2±27.7, after the operation - 61.5±21.1. The indicator of the bone-air interval before surgery in patients averaged 29.8±9.7 dB, 1 year after surgery - 13.0±10.9 dB (p>0.05). The first experience of using osteoplastic materials for obliterating the paratympanic spaces in children has shown high efficiency, ease of use and safety.