Opposite Order Research Articles

Comparatively study of the electronic structure, thermal expansivity and lattice thermal conductivity of CaOn (n = 1, 2, 3)

Recent studies reported that Ca and O can form calcium peroxide (CaO2) and calcium ozonide (CaO3) at high pressure, while the knowledge of their physical properties is limited. In this study, the first principles were adopted to investigate the electronic structure, thermal expansivity (α) and lattice thermal conductivity (κlatt) of CaOn. The density of states (DOS) show that three compounds are semiconductors, and the split of O 2s orbitals are taken place owing to the O–O bonds in CaO2 and CaO3. Meanwhile, the contribution of the lowest conduction band varies significantly with the change of the number of O atom. The calculations indicate that α is in the order of αCaO3>αCaO2>αCaO, which is majorly determined by the Grüneisen constant (γ). The calculated κlatt shows an opposite order to α. The further discussion unveils that the anharmonic scattering rates of three compounds account for the magnitude of κlatt.

Influence of moderate hypothermia on renal and cerebral haemodynamics and oxygenation during experimental cardiopulmonary bypass in sheep.

Cardiac surgery requiring cardiopulmonary bypass (CPB) can result in renal and cerebral injury. Intraoperative tissue hypoxia could contribute to such organ injury. Hypothermia, however, may alleviate organ hypoxia. Therefore, we tested whether moderate hypothermia (30°C) improves cerebral and renal tissue perfusion and oxygenation during ovine CPB. Ten sheep were studied while conscious, under stable anesthesia, and during 3h of CPB. In a randomized within-animal cross-over design, five sheep commenced CPB at a target body temperature of 30°C (moderate hypothermia). After 90 min, the body temperature was increased to 36°C (standard procedure). The remaining five sheep were randomized to the opposite order of target body temperature. Compared with the standard procedure, moderately hypothermic CPB reduced renal oxygen delivery (-34.8% ± 19.6%, P=0.003) and renal oxygen consumption (-42.7% ± 35.2%, P=0.04). Nevertheless, moderately hypothermic CPB did not significantly alter either renal cortical or medullary tissue PO2 . Moderately hypothermic CPB also did not significantly alter cerebral perfusion, cerebral tissue PO2 , or cerebral oxygen saturation compared with the standard procedure. Compared with the anesthetized state, the standard procedure reduced renal medullary PO2 (-21.0 ± 13.8 mmHg, P=0.014) and cerebral oxygen saturation (65.0% ± 7.0% to 55.4% ± 9.6%, P=0.022) but did not significantly alter either renal cortical or cerebral PO2 . Ovine experimental CPB leads to renal medullary tissue hypoxia. Moderately hypothermic CPB did not improve cerebral or renal tissue oxygenation. In the kidney, this is probably because renal tissue oxygen consumption is matched by reduced renal oxygen delivery.

Asynchronization at the phonology-morphology interface: A case study of an atypically developing Hebrew-acquiring boy

ABSTRACT In this paper, we present a case study of an atypically developing Hebrew-acquiring boy (YV), in comparison with three typically developing boys. Drawing on data from longitudinal studies, we examined the development of two verbal suffixes, -im ‘ms.pl.pres’ and -ti ‘1.sg.past’, with reference to two prosodic structures that these suffixes assume – word final codas for -im and trisyllabic words for -ti (e.g. bon-ím ‘they build’, kará-ti ‘I read’). We found that YV’s developmental trajectory was similar to that of the three boys in both phonology and morphology, each module independently; the deviant phenomena were found at the interface between phonology and morphology. The typically developing boys produced the relevant phonological structures in bare stems before they produced them in suffixed forms. YV, however, proceeded in the opposite order; he produced the final m in -im verbs before mastering word final codas in bare stems; similarly, he produced trisyllabic forms in -ti verbs before mastering them in bare stems. We attribute this deviance to asynchronization between phonological (prosodic) and morphological development. That is, YV’s phonology lagged behind his morphology, but this lag did not block the morphological development as it would have in synchronized development.

High-accuracy circular dichroism measurement using a liquid crystal polarization grating

A significant improvement in the accuracy of a circular dichroism (CD) measurement system using a liquid crystal polarization grating (LCPG) for broadband applications can be achieved. The influences of the diffraction efficiency and residue from the opposite diffraction order for an LCPG are studied theoretically and experimentally. The results show that the measurement accuracy is highly dependent on the residue. The diffraction efficiency only affects the measurement resolution. We also find that when an LCPG is operated at a wavelength far from the design wavelength, the LCPG has a high residue, which will generate a significant measurement error. Five lasers with different wavelengths ranging from visible to infrared are applied to investigate the accuracy of our proposed system for broadband applications. The measurement error can be reduced by one order of magnitude for wavelengths far from the design wavelength of an LCPG by subtracting the residue effect. The final measurement accuracy of our CD apparatus is determined to be a few mdeg as shown in a comparison of the measurement results for a vitamin B12 solution with those obtained using a commercial CD spectropolarimeter.

(Invited) Theoretical Study on the Excited and Charge-Separated States of Donor-Acceptor-Linked Molecules

Photoinduced charge separation process plays important roles in various fields such as natural and artificial photosynthesis, solar cell, and light-emitting diode. Toward an understanding of the molecular mechanism of photoinduced charge separation, we have been studying the excited and charge-separated states of donor-acceptor-linked molecules with theoretical methods such as QM/MM method and MD simulation. In this presentation, we will mainly introduce two topics of our research. The first one is porphyrin-fullerene linked molecules. It is experimentally known that the yields of charge-separated states of these molecules is not linear with the bridge length, but reaches a maximum at a specific length. It is also known that the energy of the singlet excited state of this compound is lower in benzonitrile solvent in the following order: local excited state of porphyrin, local excited state of fullerene, and charge separation state. However, by simply using the stable structure of the ground state in the theoretical calculations, the energies of each excited state are in the exact opposite order, and thus the experimental results could not be reproduced. In order to reproduce the experimental results, the structural relaxation of the molecule in the excited state and the response of the solvent are important. However, a simple polarization continuum model of the solvent is insufficient to stabilize the charge separation state. Therefore, we used the QM/MM RWFE-SCF method, which efficiently combines the QM/MM method with MD simulations to optimize the geometry of the solute molecule (QM region) with quantum chemical calculations including the effects of the thermal fluctuations of the surrounding environment such as the solvent molecules (MM region) treated by the molecular mechanics force field. The energy differences calculated with the QM/MM RWFE-SCF method are in good agreement with the experimental results. The charge-separated state is greatly stabilized by solvent molecules due to the large dipole moment. The next topic is acceptor-donor-acceptor type non-fullerene acceptor called TACIC. The excited state of TACIC is known to have a longer lifetime in the film state than in solution. This is in contrast to a similar non-fullerene acceptor molecule, ITIC, which has a shorter lifetime in the film state. However, the molecular mechanism is unknown. We analyzed the structures and excited states of the TACIC and ITIC by using quantum chemical calculations and MD simulation. It is found that the packing structure of TACIC in the film state is much different from that of ITIC, which could be related to the difference in lifetime of the excited state. Further analysis is currently in progress.

Unveiling the Complex Magnetization Reversal Process in 3D Nickel Nanowire Networks

AbstractUnderstanding the interactions among magnetic nanostructures is one of the key factors to predict and control the advanced functionalities of 3D integrated magnetic nanostructures. In this work, the focus is on different interconnected Ni nanowires forming an intricate, but controlled, and ordered magnetic system: Ni 3D Nanowire Networks (3DNNs). These self‐ordered systems present striking anisotropic magnetic responses, depending on the interconnections’ position between nanowires. To understand their collective magnetic behavior, the magnetization reversal processes are studied within different Ni 3D Nanowire Networks compared to the 1D nanowire 1DNW array counterparts. The systems are characterized at different angles using first magnetization curves, hysteresis loops, and First Order Reversal Curves techniques, which provided information about the key features that enable macroscopic tuning of the magnetic properties of the 3D nanostructures. In addition, micromagnetic simulations endorse the experiments, providing accurate modeling of their magnetic behavior. The results reveal a plethora of magnetic interactions, neither evident nor intuitive, which are the main role players controlling the collective response of the system. The results pave the way for the design and realization of 3D novel metamaterials and devices based on the nucleation and propagation of ferromagnetic domain walls both in 3D self‐ordered systems and future nano‐lithographed devices.

Plant growth and heavy meal accumulation characteristics of Spathiphyllum kochii cultured in three soil extractions with and without silicate supplementation

A hydroponic method was conducted to test whether Spathiphyllum kochii is tolerant to multiple HMs as well as to evaluate whether sodium silicate promotes plant growth and alleviates HM stress mainly by assessing biomass, HM accumulation characteristics and antioxidant enzyme activities (AEAs). Three soil extractions from an uncontaminated soil, a comparable lightly HM-contaminated soil (EnSE), and a comparable heavily HM-contaminated soil (ExSE) with or without 1 mM sodium silicate supplementation were used. S. kochii showed no obvious symptoms when cultured in EnSE and ExSE, indicating that it was a multi-HM-tolerant species. The biomass and photosynthesis followed the order: UnSE > EnSE > ExSE, but the opposite order was found for HM concentration, AEAs, and malondialdehyde content. Silicate had no effects on the growth and HM bioaccumulation characteristics of S. kochii cultured in UnSE but exhibited a novel role in decreasing HM uptake by 13.61–41.51% in EnSE and ExSE, respectively, corresponding upregulated AEAs, and reduced malondialdehyde contents, resulting in increased biomass and alleviating HM stress. The activities of peroxidase and superoxide dismutase were upregulated by an increase in soil extraction HM concentration and further upregulated by silicate supplementation, indicating that they were important mechanisms alleviating HM stress in S. kochii.

Brain Functional Networks Involved in Different Premise Order in Conditional Reasoning: A Dynamic Causal Model Study

In conditional reasoning, the reasoner must draw a conclusion based on a conditional or "If…, then…" proposition. Previous studies have reported that reversing the premises can effectively promote modus tollens reasoning (a form of conditional reasoning), but subsequent experimental studies have found no such effect. Therefore, to further examine this issue and reveal the cognitive mechanism of conditional reasoning, we asked two groups of healthy volunteers (traditional and inverted premise order groups) to evaluate a set of visually presented conditional tasks (modus ponens/modus tollens) under fMRI. The results indicated that the inverted condition activated more brain regions associated with working memory, including the angular gyrus (BA 39), precuneus (BA 7), inferior parietal lobe, and middle frontal gyrus. The resulting common activation map was used to define the ROIs and perform dynamic causal modeling for the effective connectivity analysis, containing the medial frontal gyrus, hippocampus, cerebellum, and middle occipital gyrus in the right hemisphere and the inferior occipital gyrus in the left hemisphere. The results of intrinsic connections in the optimal model selected by Bayesian model selection showed that the connection strength was stronger in the inverted group rather than in the traditional group, which may indicate that the reversal of the premise order promotes connectivity between brain regions. Despite the lack of a premise order effect, we did discover a neuronal separation between the inverted and traditional conditions, which lends support to the mental model theory to some extent.

Soot Oxidation over γ-Al2O3-Supported Manganese-Based Binary Catalyst in a Dielectric Barrier Discharge Reactor

In this work, soot oxidation was conducted over a series of Mn-X/γ-Al2O3 (M = Ce, Co and Cu) binary catalysts in a dielectric barrier discharge reactor. The soot conversion in the plasma–catalytic system was in the order of Mn/γ-Al2O3 (57.7%) > Mn-Co/γ-Al2O3 (53.9%) > Mn-Ce/γ-Al2O3 (51.6%) > Mn-Cu/γ-Al2O3 (47.7%) during the 30 min soot oxidation process at 14 W and 150 °C. Meanwhile, the doping of Ce, Co and Cu slightly improved the CO2 selectivity of the process by 4.7% to 10.3% compared to soot oxidation over Mn/γ-Al2O3.It is worth to note that the order of CO2 selectivity was in the opposite order with soot oxidation rate. The effects of discharge power, oxygen content in the carrier gas and reaction temperature on plasma–catalytic soot oxidation was systematically analyzed. The catalyst characterizations, including N2 adsorption–desorption, X-ray diffraction, X-ray photoelectron spectroscopy, temperature-programmed reduction by H2 and temperature-programmed desorption of O2, were conducted to illustrate the reaction mechanisms of plasma–catalytic soot oxidation and reaction pathways.

Routine contrast enema prior to stoma reversal seems only required following treatment for necrotizing enterocolitis: An evaluation of the diagnostic accuracy of the contrast enema

IntroductionContrast enemas are often made prior to stoma reversal in order to detect distal intestinal strictures distal of the stoma. If untreated these strictures can cause obstruction which might necessitate redo-surgery. However, the value of contrast enemas is unclear. Therefore, we aim to evaluate the contrast enema's diagnostic accuracy in detecting strictures in children with a stoma. MethodsYoung children (≤3 years) treated with a stoma between 1998 and 2018 were retrospectively included. The STARD criteria were followed. Patients treated for anorectal malformations and those that died before stoma reversal were excluded. Surgical identification of strictures during reversal or redo-surgery within three months was used as gold standard. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and area under the curve (AUC) reflected diagnostic accuracy. ResultsIn 224 included children, strictures were found during reversal in 10% of which 95% in patients treated for necrotizing enterocolitis. Contrast enema was performed in 68% of all patients and detected 92% of the strictures. In the overall cohort, the sensitivity was 100%, specificity 98%, PPV 88% and NPV 100% whilst the AUC was 0.98. In patients treated for NEC, the sensitivity was 100%, specificity 97%, PPV 88% and NPV 100% whilst the AUC was 0.98. ConclusionStrictures prior to stoma reversal seem to be mainly identified in patients treated for NEC and not in other diseases necessitating a stoma. Moreover, the contrast enema shows excellent diagnostic accuracy in detecting these strictures. For this reason we advise to only perform contrast enemas in patients treated for NEC. Level of EvidenceII

Characterization of Plant Accumulation of Pharmaceuticals from Soils with Their Concentration in Soil Pore Water.

Predicting plant uptake of pharmaceuticals from soils is very challenging because many pharmaceuticals are ionizable compounds, which experience highly variable sorption/desorption and transformation processes in soils. This study aimed to elucidate how the equilibrium between sorbed and dissolved phases influences radish uptake of 15 pharmaceuticals from three soils with different properties. After 30 days of uptake, the accumulation of acetaminophen, carbamazepine, lamotrigine, carbadox, trimethoprim, and triclosan in radish ranked as Riddles > Capac > Spinks soil. In contrast, radish accumulation of caffeine, lincomycin, monensin, tylosin, sulfadiazine, and sulfamethoxazole exhibited the opposite order of Riddles < Capac < Spinks soil. Oxytetracycline and estrone demonstrated similar accumulation in radish grown in the three soils. Accumulation of pharmaceuticals in radish demonstrated no apparent relation with their concentration in soils. However, we identified strong positive correlation between pharmaceutical accumulation in radish and their corresponding concentration in soil pore water. These results reveal that pharmaceutical in soil pore water is the dominant fraction bioavailable to plant uptake. Relatively constant root concentration factors (RCFs) on the basis of pharmaceutical concentration in soil pore water, compared to the highly variable RCFs derived from soils, suggest that pore water-based RCF is superior for describing pharmaceutical accumulation in plants grown in soils. We recommend that pharmaceuticals in soil pore water should be evaluated and included in modeling their uptake by plants.

Soot Oxidation in a Plasma-Catalytic Reactor: A Case Study of Zeolite-Supported Vanadium Catalysts

The plasma-catalytic oxidation of soot was studied over zeolite-supported vanadium catalysts, while four types of zeolites (MCM-41, mordenite, USY and 5A) were used as catalyst supports. The soot oxidation rate followed the order of V/MCM-41 &gt; V/mordenite &gt; V/USY &gt; V/5A, while 100% soot oxidation was achieved at 54th min of reaction over V/MCM-41 and V/mordenite. The CO2 selectivity of the process follows the opposite order of oxidation rate over the V/M catalyst. A wide range of catalyst characterizations including N2 adsorption–desorption, XRD, XPS, H2-TPR and O2-TPD were performed to obtain insights regarding the reaction mechanisms of soot oxidation in plasma-catalytic systems. The redox properties were recognized to be crucial for the soot oxidation process. The effects of discharge power, gas flow rate and reaction temperature on soot oxidation were also investigated. The results showed that higher discharge power, higher gas flow rate and lower reaction temperature were beneficial for soot oxidation rate. However, these factors would impose a negative effect on CO2 selectivity. The proposed “plasma-catalysis” method possessed the unique advantages of quick response, mild operation conditions and system compactness. The method could be potentially applied for the regeneration of diesel particulate filters (DPF) at low temperatures and contribute to the the emission control of diesel engines.

Accelerating binary black hole system

Recently, by the end of their second observing run, advanced LIGO and Virgo have detected some binary black hole mergers. In this paper, we show that the acceleration of black holes around each other causes the emergence of two Rindler space–times. The parameters of the black hole in each region change in opposite order to the parameters of the black hole in the other region. Also, the acceleration has a direct relation to the black hole entropies and their observed redshifts.

A Recipe for Disappointment: Policy, Effect Size, and the Winner’s Curse

Evidence-based education aims to support policy makers choosing between potential interventions. This rarely involves considering each in isolation; instead, sets of evidence regarding many potential policy interventions are considered. Filtering a set on any quantity measured with error risks the “winner’s curse”: conditional on selecting higher valued measures, the measurement likely overestimates the latent value. This article explains the winner’s curse, illustrates it for one constrained and complete set of educational trials—the UK’s Education Endowment Foundation’s projects, where evidence is summarized with standardized effect size—and shows the results of adjusting for the curse on this set. This analysis suggests selecting policies for higher effect size can result in substantial effect size inflation and in some cases order reversals: one intervention ranking above another on estimated effect size but below it when adjusted. The issue has implications for evaluation programs, power analyses, and policy decisions. For example, even in the absence of other problems with interpreting effect size, it can help explain why policies tend to deliver less than promised.

Systematic study of nuclear effects in p+Al, p+Au, d+Au , and He3+Au collisions at sNN=200 GeV using π0 production

The PHENIX Collaboration presents a systematic study of inclusive π0 production from p+p, p+Al, p+Au, d+Au, and He3+Au collisions at sNN=200GeV. Measurements were performed with different centrality selections as well as the total inelastic, 0–100%, selection for all collision systems. For 0–100% collisions, the nuclear-modification factors, RxA, are consistent with unity for pT above 8GeV/c, but exhibit an enhancement in peripheral collisions and a suppression in central collisions. The enhancement and suppression characteristics are similar for all systems for the same centrality class. It is shown that for high-pT−π0 production, the nucleons in the d and He3 interact mostly independently with the Au nucleus and that the counterintuitive centrality dependence is likely due to a physical correlation between multiplicity and the presence of a hard scattering process. These observations disfavor models where parton energy loss has a significant contribution to nuclear modifications in small systems. Nuclear modifications at lower pT resemble the Cronin effect—an increase followed by a peak in central or inelastic collisions and a plateau in peripheral collisions. The peak height has a characteristic ordering by system size as p+Au>d+Au>He3+Au>p+Al. For collisions with Au ions, current calculations based on initial-state cold nuclear matter effects result in the opposite order, suggesting the presence of other contributions to nuclear modifications, in particular at lower pT.6 MoreReceived 11 November 2021Accepted 3 May 2022DOI:https://doi.org/10.1103/PhysRevC.105.064902©2022 American Physical SocietyPhysics Subject Headings (PhySH)Research AreasHadronic decaysLeptonic, semileptonic & radiative decaysParticle & resonance productionParticle decaysRelativistic heavy-ion collisionsPhysical SystemsMesonsTechniquesHadron collidersNuclear Physics

The interplay of hedonic trend and time pressure in the evaluation of multi-episode tour experiences

Tour experiences often comprise sequences of episodes, yet little is known in tourism research on how two common situational factors might alter individuals' evaluation of such multi-episode experiences: hedonic trend (i.e., the order of such episodes) and perceived time pressure (i.e., individuals' perception of limited available time). Two studies (i.e., an online experiment on a multi-city tour and a field experiment in a multi-site archaeological park) examine the interaction between these two factors by showing that individuals exhibit better evaluative responses (i.e., liking and revisit intention) to multi-episode tour experiences when such experiences have an ascending hedonic trend (i.e., their constituting episodes unfold in an increasing attractiveness order) rather than a descending one (i.e., the same episodes unfold in the opposite order). Importantly, individuals' perception of time pressure reverses this tendency. Our findings carry theoretical and managerial implications on how to design multi-episode tour experiences.

A collective interpretation semantics for reversing Petri nets

Reversing Petri nets (RPNs) have recently been proposed as a net-based approach to model causal and out-of-causal order reversibility. They are based on the notion of individual tokens that can be connected together via bonds. In this paper we extend RPNs by allowing multiple tokens of the same type to exist within a net and we implement a notion of local reversibility for the model based on the collective token interpretation of Petri nets. The resulting framework allows us to model a form of out-of-causal-order reversibility, a type of reversibility that arises most notably in biological systems. Furthermore, it is lightweight in the sense that it requires no global control and no history needs to be preserved. Furthermore, we provide a mechanism for controlling reversibility by associating transitions with conditions, which guard their execution. We demonstrate the usefulness of the framework by modelling the functioning of the sodium-potassium pump, a system where transition execution is governed by activation conditions and featuring reversible behaviour.

Interphase exchange coupling and magnetocaloric effect in Co3Gd4/Co7Gd12 magnetic nanocomposites, obtained by mechanical milling

The effect of interphase exchange coupling on the Curie temperature of mechanically milled Co3Gd4/Co7Gd12 magnetic nanocomposites was studied. It was shown that the nanocomposites have a single critical temperature (195 K), approximately half way between those of the constituent alloys (160 K for Co7Gd12 and 220 K for Co3Gd4). The interphase exchange coupling within the nanocomposites was investigated, at 4 K, by the First Order Reversal Curve method.

Magnetoactive elastomers based on ferromagnetic and ferroelectric particles: A FORC approach

Composites based on ferromagnetic (FM) microparticles or a mixture of FM and ferroelectric (FE) microparticles embedded into a soft polymer matrix were studied. This class of materials belongs to magnetoactive elastomers (MAE) which received renewed interest stimulated by breakthroughs in their applications, for example, in soft robots and biomedical technologies. Moreover, the MAE with a mixture of FM and FE particles exhibit multiferroic properties which open new ways for their application. Here, the magnetic properties of this type of material were studied using the First Order Reversal Curve (FORC) method in terms of local distributions of the switching fields and interaction fields. In particular, the influence of FE particles on the magnetization reversal processes was determined. The latter is related to the influence of FE particles on the structural properties of the polymer and the possibility of displacement of FM particles in the matrix.

Abstract B019: Does order matter? Dissecting the single-cell differences underlying resistance to sequential drug treatment

Abstract Most cancer patients are given multiple different drugs during the course of their cancer treatment. An important but outstanding question is the role that drug order plays in the development of resistance to sequential drug treatment– that is, if cells that develop resistance to a certain first and second-line therapy are similar to cells that develop resistance to the same drugs given in the opposite order. During treatment with a first drug, both selection and transcriptional reprogramming events alter the cells within a tumor. As such, surviving cells may exhibit increased sensitivity or increased resistance to future drug therapies. Here, we zoom in to the single-cell level using cell barcoding and sequencing and explicitly test whether the order of treatment leads to different populations of cells developing resistance to a panel of different therapies. As the initial application of drug therapy changes a tumor in multiple ways, it is currently unclear which changes are most influential in the overall modification of a tumor’s sensitivity to a second-line therapy. One hypothesis is that changes in sensitivity are due to specific drug-induced modifications in the characteristics of cells that survived the first-line therapy, in which case order may matter. An alternative hypothesis is that changes in sensitivity are simply the result of a decrease in clonal diversity within the tumor, where the ability of individual subpopulations to survive the second-line therapy is unchanged by the first-line treatment, in which case order may not matter. To distinguish between these hypotheses, we have combined single-cell RNA sequencing with cellular barcoding to assess both the survival and transcriptome of cells from over 100,000 cells over the course of treatment with multiple stressors. These stressors include a combination of targeted inhibitors (Dabrafenib and Trametinib), chemotherapy (Cisplatin), and hypoxic stress (CoCl2). Through analysis of the transcriptome of cells within the same lineage after surviving each therapy in the sequential treatment scheme, we are now working to determine whether development of resistance to a first-line therapy causes individual cells within a lineage to change in ways that alter their sensitivity to subsequent therapies. Should we find that developing resistance to first-line therapy does increase resistance to specific second-line therapies, we will use our sequencing data to identify specific transcriptomic changes which lead to this induced resistance. We will then look to block this induced resistance through either small-molecule inhibitors (if available) or CRISPR-mediated knockout of genes that promote resistance to second-line therapies. Overall, this study will provide two methods for increasing the efficacy of sequential drug treatment through both the identification of ideal drug ordering and the targeting of changes that promote induced resistance, thus leading to resistance-free treatment strategies. Citation Format: Aria J. Fasse, Dylan L. Schaff, Sydney M. Shaffer. Does order matter? Dissecting the single-cell differences underlying resistance to sequential drug treatment [abstract]. In: Proceedings of the AACR Special Conference on the Evolutionary Dynamics in Carcinogenesis and Response to Therapy; 2022 Mar 14-17. Philadelphia (PA): AACR; Cancer Res 2022;82(10 Suppl):Abstract nr B019.