Oral Fluid Of Patients Research Articles

Визначення концентрації метаболітів азоту в плазмі крові та ротовій рідині у пацієнтів з захворюваннями тканин пародонту на тлі загальносоматичних захворювань

Визначення концентрації метаболітів азоту в плазмі крові та ротовій рідині у пацієнтів з захворюваннями тканин пародонту на тлі загальносоматичних захворювань

Comparative analysis of microbiota of oral fluid in patients with psychoneurological disorders using toothpaste with the enzyme «bromelain»

Relevance. The complex of actions for decrease in growth of diseases of the maxillofacial region includes the reorganization of the mouth, creating an individual program for hygiene and prevention. Among the large number of types of therapeutic and preventive hygiene products for patients with neuropsychiatric disorders, given the burdened somatic status, the program should include safe for use basic and additional means.Purpose. Comparative assessment of oral fluid microorganisms in patients with psychoneurotic disorders before and after 6 months of using toothpastes with the enzyme «bromelain».Materials and methods. A comparative analysis of the oral fluid microflora in patients with high intensity and activity of the cariosity, with positive (psychoneurological disorders) anamnesis and without comorbidities has been carried out. The mouths of all studied patients were sanitized, and a program of personal oral hygiene using hygienic toothpastes and with the enzyme «bromelain» was developed.Results. The conducted research does not prove the bactericidal effect of the enzyme «bromelain» on oral microflora, however, a significant decrease of the total number of viable bacteria, changes in the species composition, and the degree of hemolecular properties indicates an indirect bacteriostatic effect of this enzyme in the composition of the toothpastes, which can be considered as a positive effect on the decrease of caries activity in patients with «negative» dental status (caries intensity index > 9, hygiene index by the method of Yu.A. Fedorov and V.V. Volodkina > 2 points, 3rd degree of caries activity by T.F. Vinogradova), including in patients with psychoneurological disorders.Conclusion. The use of enzyme «bromelain» in toothpastes in patients with neuropsychiatric disorders contributes not only to the safe and intensive cleavage of soft plaque, but also to a positive effect on the microbiota of the oral fluid, which is a favorable prognostic criterion for reducing the activity of the carious process.

Assessment of endogenous intoxication values by oral fluid composition

To study changes in the parameters of endogenous intoxication using the level of substances of low and medium molecular weight in the oral fluid in patients with catarrhal stomatitis, which got developed after the acute drug poisoning. To determine the degree of intoxication, we studied the concentrations of low and medium molecular weight substances (SLMMW) in the oral fluid of patients. We studied the saliva of 20 patients (13 men and 7 women, average age 39 years). The control was done against the oral fluid of 10 healthy individuals. The studies were conducted in a control group consisting of 10 healthy individuals with intact oral mucosa and two groups of patients (20 patients in total) with drug poisoning. The first of these two groups of patients included 8 individuals without pathological changes in the oral mucosa, while the second group consisted of 12 patients diagnosed with catarrhal stomatitis that developed after acute drug poisoning. It became known that endogenous intoxication develops in the oral fluid of patients with acute drug poisoning, which is manifested by a statistically significant increase in the content of SLMMW in comparison with practically healthy individuals (15.3%). And the total level of SLMMW in the oral fluid of patients with acute drug stomatitis and catarrhal stomatitis has increased by 42.3% relative to the data of practically healthy individuals. Studies have shown the need for monitoring the oral cavity in patients with acute drug poisoning, with the aim of timely detection of pathologies of the oral mucosa in them and the appointment of complex treatment.

Biofilm-forming bacteria in atherosclerotic plaques in patients with cardiovascular diseases and chronic periodontitis

The identification of viable, previously undetectable types of microbes in human atherosclerotic plaques provides new evidence of direct and indirect participation of microbiota in the etiopathogenesis of non-infectious human diseases. Purpose of the study. Isolation and determination of the composition of viable microbes in atherosclerotic plaques and comparative analysis with the microbiota of the oral fluid in patients with chronic generalized periodontitis (CP) and coronary artery disease (CAD). Materials and methods. Fragments of atherosclerotic plaques isolated during coronary artery bypass grafting in patients with coronary artery disease and chronic periodontitis were cultured in a fluid system under anaerobic conditions. Isolated microbes were identified using bacteriological methods of research and polymerase chain reaction. The presence of biofilms was evaluated by scanning electron microscopy. Results. Twelve patients with CP and CAD were examined. Biofilms containing genetic markers of at least one type of microbiota were isolated from all atherosclerotic plaques after cultivation in a fluid system and anaerobic conditions. They included DNA of periodontal pathogens, some pathogens of purulent-inflammatory diseases, herpesviruses and Candida spp., as well as viable microbes. In the examined patients, a comparative analysis of the composition of the isolated microbiota from atherosclerotic plaques and oral fluid microbes was performed. Conclusions. A method for modeling biofilms based on fluid technology allows detecting previously undetectable types of microbiota in the walls of blood vessels and atherosclerotic plaques in sufficient quantities to identify and study their properties.

Цитокіновий статус хворих на генералізований пародонтит на тлі цукрового діабету II типу

Introduction. The problem of generalized periodontitis is taking on particular significance due to the data concerning its close connection with somatic diseases, among which diabetes mellitus is particularly important. In studying the pathogenesis of generalized periodontitis in the settings of diabetes mellitus, it is important to determine the synergistic effect of proinflammatory cytokines, which can be markers of local changes and a diagnostic criterion for the severity and activity of the pathological process in the periodontal tissues. The aim of the study was to determine the cytokine profile of oral fluid in patients with generalized periodontitis in the settings of type II diabetes depending on the severity and course of the dystrophic-inflammatory process in periodontal tissues. Materials and methods. To study this issue, a comprehensive clinical and immunological study of 41 patients with generalized periodontitis in the settings of type II diabetes and 10 people with clinically intact periodontal tissues was performed. Results. The development of the pathological process in patients with generalized periodontitis in the settings of diabetes mellitus is accompanied by an increase in the concentration of proinflammatory cytokines in the oral fluid, namely TNF-α, IL-1β, IL-6, which contribute to the progression of dystrophic inflammation in periodontal tissues. The mechanism of pathogenetic correlations substantiates the scientific approach to the choice and application of immunological methods for the study of patients with generalized periodontitis. Conclusions. Level detection of TNF-α, IL-1β, IL-6 pro-inflammatory cytokines in the oral fluid can be a diagnostically important factor in determining the severity of the pathological process and the effectiveness of treatment of patients with generalized periodontitis.

Assessment of the efficiency of sialosis treatment in patients with type 2 diabetes mellitus

Was to determine changes in the levels of cytokines in the oral fluid in patients with type 2 diabetes and sialosis, depending on the level of glycemia and the duration of type 2 diabetes, as well as in the dynamics of basic and complex treatment. Forty patients with type 2 diabetes were enrolled in the study and divided into two groups according to disease duration: group 1 comprised 15 patients having diabetes for ≤5 years, group 2 - 25 suffering for more than 5 years. The study revealed changes in pancreatic cancer immunological parameters in patients with sialosis and type 2 diabetes. Long course of diabetes led to an increase in the level of IL-2 and a decrease in IFN-γ in oral fluid. The level of IL-1β was the highest in the group of patients with a duration of diabetes ≤5 years. According to the data presented, the content of IL-1β, IL-2 in patients with diabetes is higher, and INF-γ is lower than in healthy individuals. In patients with sialosis on the background of type 2 diabetes before treatment there is a decrease in the level of IFN-γ in the oral fluid and its increase to 7.3±0.2 pg/ml after treatment. At the same time, when enzyme therapy was included in the treatment regimen, the increase in the level of IFN-γ in the main group was more pronounced and averaged 8.3±0.1 pg/ml, which was 1.2 times higher than the initial level (p<0,05). The inclusion of enzyme therapy in the complex therapy of patients with sialosis against the background of diabets, unlike traditional methods of therapy, made it possible to provide faster positive clinical dynamics, namely, under the influence of this drug, the pain stopped in a short time, salivation increased and the feeling of dryness disappeared. Along with a faster relief of clinical symptoms, normalization of the levels of IL-1β, IL-2 and an increase in IFN-γ by the end of treatment were noted.

PECULIARITIES OF PRO- AND ANTI-INFLAMMATION CYTOCINS LEVEL IN PREVENTION OF PROSTHETIC STOMATITIS IN PATIENTS WITH REMOVABLE ACRYLIC PROSTHESES

Due to the using of removable laminar dentures made of acrylic plastic, the complications in the form of chronic inflammation of the oral mucosa are developed in a certain category of patients, as a result of the complex impact of dentures on the tissues of the prosthetic bed in terms of mechanical, chemical, toxic, microbiological and immunological factors. In conditions of mechanical or chemical damage to the mucous membrane epithelium by the action of the microbial factor the activation of epithelial cells occurs and they acquire the properties of immunocompetent cells. As a result, the epithelial cells begin to produce cytokines. A special place in the implementation of acute inflammatory process is held by proinflammatory cytokines: interleukin (IL-1β) and tumor necrosis factor (TNF-α). They regulate all successive stages in the development of inflammation and an adequate response to the etiological factor. Interleukin 10 (IL-10) is the main anti-inflammatory cytokine, which inhibits the synthesis of proinflammatory cytokines. Determination of the level of pro- and anti-inflammatory cytokines is a diagnostic criterion in the development of prosthetic stomatitis and at the same time it is used to test the effectiveness of the proposed method of prevention or treatment of complications on the part of the tissues of the prosthetic bed.&#x0D; The aim of the research is to study the concentration of proinflammatory cytokines (TNFα, IL-1β) and anti-inflammatory cytokines (IL-10) in the oral fluid of patients with complete and partial defects of the dentition after prosthetics with removable laminar dentures made of acrylic plastic and dentures coated with fullerene C60 molecules.&#x0D; Material and methods of research. The study included 39 patients who were between 40 to 80 years old, including 19 male (48.7%) and 20 female (51.3%) subjects. The partial and complete defects of the dentition were diagnosed. The control group consisted of apparently healthy individuals, namely 10 people without defects of the dentition and periodontal tissue diseases.&#x0D; The patients were divided into 3 groups: the first group comprised apparently healthy people with intact dentition; the second group included patients with complete and partial defects of the dentition after prosthetics with removable laminar dentures made of acrylic plastic (20 patients) 7 days after prosthetics; the third group – patients with complete and partial defects of the dentition with removable laminar dentures made of acrylic plastic (20 patients) 30 days after prosthetics; the fourth group – patients with complete and partial defects of the dentition after prosthetics with removable laminar dentures made of acrylic plastic and coated with fullerene C60 molecules.&#x0D; The concentration of cytokines IL-1β and TNFα and IL-10 (pg / ml) was determined in the mouth before treatment at day 7, 2 weeks and 1 month. Statistical processing was conducted using STATISTICA 6.0 (StatSoft, USA).&#x0D; Results of the studies and their discussion. The results of the studies show that the level of cytokines in patients before prosthetics is higher than in apparently healthy people. On the 7th and 30th day after prosthetics in the study groups, there was a sharp increase in the concentration of proinflammatory and anti-inflammatory cytokines in the oral fluid as contrasted with the comparison group. These data confirm that activation of local synthesis of proinflammatory cytokines in the oral fluid is an important pathogenetic factor that determines the formation and maintenance of an active inflammatory response in the mucosa of the prosthetic bed. After fullerene C60 coating of the surface of denture, which is in contact with the tissues of the prosthetic bed, there is a significant decrease in the level of proinflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines.

Comparative analysis of biophysical parameters of oral fluid depending on the condition of dental hard tissue

The pH, buffer capacity, salivation rate, impedance, micellarity and mineralizing ability of the oral fluid have been determined by the indices of the dispersion coefficient. The dispersion coefficients impedance of oral fluid in patients with a wedge-shaped defect and precervical caries were significantly lower than in patients with intact hard tissues (p=0,004). The greatest steepness of dispersion coefficient was typical for the oral fluid of individuals without any precervical pathology, which corresponds to a high degree of its liquid crystal phase, greater dispersion and micellarity. The strong indirect correlation between impedance and pH and buffer capacity has been revealed and the vivid direct correlation with salivation rate and dispersion coefficient has been found. The interconnections between biophysical parameters of oral fluid require further research in order to diagnose precervical pathology and monitor the effectiveness of preventive measures.

Evaluation of the qualitative and quantitative composition of or al microbiota in patients with medial osteonecrosis of the jaw

Aim. To determine the qualitative and quantitative composition of the oral microbiota in patients with medial osteonecrosis of the jaw (MOJ). Materials and methods. Microbiota was evaluated in 15 patients with medial osteonecrosis of the jaw. An assessment was made of 12 groups of optional and obligate-anaerobic microorganisms. Evaluation of the qualitative and quantitative composition of oral microbiota in patients with MOJ was performed by real-time polymerase chain reaction (PCR-RT). Results. The aerobic-anaerobic microbiota composition in patients with MOJ in the oral fluid and separated from the fistula is presented. The values of the total bacterial mass (TBM) and all anaerobic microorganisms in the oral fluid in patients with MOJ before treatment were higher by 1-3 orders of magnitude than in healthy individuals. In the jaw, separated from the fistula in the zone of necrosis of the jaw, these parameters were higher by 2-4 orders than in the oral fluid. Prior to surgery, a course of antibiotic therapy was conducted for 7 days. As a result of the therapy, the parameters of anaerobic microflora in the oral fluid decreased. The use of cefazolin was more effective than lincomycin. In 1 year after surgical treatment, the index of TBM and the indices of anaerobic microorganisms did not differ from those of healthy individuals. Conclusion. Patients with medical osteonecrosis of the jaw have a high level of anaerobic pathogens in the oral microbiota. The course of antibiotic therapy and surgical intervention are effective methods of treatment of MOJ, as they lead to effective sanation, and allow further endoprosthetics.

ANALYSIS OF THE ANNEXIN V APOPTOSIS MARKER LEVEL AND DENTAL STATUS IN PATIENTS WITH CHRONIC GENERALIZED PERODONTITIS AND BRONCHOECTATIC DISEASE

Aim: Our aim was to study and analyze the level of the annexin V apoptosis marker in the oral fluid in patients with generalized periodontitis without general somatic pathology and against the background of bronchiectasis.Materials and methods. 40 patients diagnosed with generalized periodontitis without somatic pathology and 50 patients diagnosed with generalized periodontitis and bronchiectasis were examined. The control group included 40 somatically healthy individuals with intact periodont. Indices characterizing the state of periodontal tissues, such as PMA, PI, Muhlemann, OHI-s were determined for all the examined persons. The level of the annexin V apoptosis marker was determined in the oral fluid by enzyme immunoassay.Results. It was established that the value of the indices of periodontal tissues (PMA, PI, Muhlemann, OHI-s) was statistically signifi cantly higher in the group of patients with generalized periodontitis against the background of bronchiectasis both in comparison with the control group and with the group of patients without general somatic pathology. The level of the annexin V apoptosis marker was also statistically signifi cantly higher in patients with generalized periodontitis against the background of bronchiectasis as compared with the control group and the group of patients without general somatic pathology (p&lt;0.001). According to the correlation analysis, statistically signifi cant relationships of the moderate strength between the level of annexin V and the value of the indices of periodontal tissues were found both in the group of patients without general somatic pathology and in the group of patients with generalized periodontitis against the background of bronchiectasis. At the same time, the strength of these relationships in patients with generalized periodontitis against the background of bronchiectasis was greater as compared to the group of patients without general somatic pathology.Conclusion. The level of the annexin V apoptosis marker in the oral fluid of patients with generalized periodontitis against the background of bronchiectasis is statistically signifi cantly higher (p&lt;0.001) than in patients with generalized periodontitis without general somatic pathology. The results of the correlation analysis indicate the presence of relationships between the level of annexin V and the value of periodontal tissue condition indices (PMA, PI, Muhlemann, OHI-s). Thus, increased apoptosis in generalized periodontitis against the background of bronchiectasis can act as a factor aggravating the damage to periodontal tissues.

Evaluation of IL-2, IL-10, IL-4 and ɤ-interferon levels in the oral fluids of patients with hepatitis C, B and HIV.

Oral fluid cytokine levels can vary considerably during the onset of Inflammatory Periodontitis (IP) especially in people with hepatitis C virus (HCV), hepatitis B virus (HBV) and human immunodeficiency virus (HIV). Aim of our study was to evaluate levels of oral cytokines during the onset of IP among HCV, HBV and HIV negative and positive individuals in order to evaluate local immunity state during these infections. This was a case control study with 3 groups of virally infected individuals and control group. All had IP including control group. 45 patients (51.7%) had HCV, 18 (20.7%) HBV and 24 (27.6%) HIV. For IL-2 we received significant difference for all groups compared with control -2.83; HBV-31.1 (p < 0.001), HCV-25.99 (p < 0.001) and HIV-24.57 (p < 0.001). For IL-10 significant difference was observed between control -0.94 and HCV-3.63 (p = 0.027), HBV-8.38 (15.51) groups (p < 0.001). IL-4 was significantly higher in control group 14.29 compared to HCV 0.2 (p < 0.001) and HIV 0.21 (p = 0.037) group. The adjusted analysis where we consider age as possible confounder revealed that only IL-2 significantly differs for all groups compared with control group: control vs HCV (p = 0.001); control vs HBV (p = 0.024); control vs HIV (p = 0.004). Evidence for significant differences when comparing oral fluid cytokines of individuals with HCV, HBV and HIV with non-viral individuals was more obvious for IL-2. IL-2 levels were significantly higher in all 3 groups vs non-viral group even when age is confounder.

Influence of dental prosthetics technology on the dynamics of early predictors of destructive inflammatory process in the periimplant zone

An objective assessment of the morphofunctional characteristics of the state of soft tissues and bone structures adjacent to the dental implant allows to control the dynamics of the processes of osseointegration in the jaw-dental implant system. The aim of the work was to investigate the level of the β-CrossLaps, С-reactive protein (CRP), osteocalcin markers after orthopedic treatment of patients using dental implant supported advanced dental restoration technologies, to perform a biochemical analysis of the oral fluid of patients after restoration using dental implants and new two-part dental implants. In patients of the index group (52 people), the removable prostheses with metal frame and fixing elements or the commercially available dental implant supported removable prostheses were installed. For the patients of the control group (12 people), the commercially available dental implant supported removable prostheses of acrylic plastics were constructed. For all the patients after 6 months the level of β-CrossLaps, CRP, osteocalcin markers in the oral fluid was analysed. In patients of the index group, the average content of β-CrossLaps was 0.0126 ± 0.002 ng/ml, in the control group it was 0.0147 ± 0.002 ng/ml. The average content of the CRP in patients of the index group was 0.358 ± 0.019 mg/l, in patients of the control group it was 0.78 ± 0.01 mg/l. In patients of the index group, the average content of osteocalcin was 1.46 ± 0.25 ng/ml, in the control group it was 1.98 ± 0.31 ng/ml. It has been shown that biochemical markers of the oral fluid can be used to predict complications after the dental implants installation. Restoration with two-part dental implants of modern design is associated with fewer complications.

Correction of indicators of oral fluid in patients with bronchocardial pathology

The oral pathology treatment against the background of somatic diseases is important problem of modern dentistry. It is known that negative drug effects of somatic diseases basic therapy have different clinical manifestations in the oral cavity and depend on the route of administration, dose, duration of use. Adverse systemic drugs effects are quite various, however some of them are more prevalent than others, and manifest themselves as influence on saliva and salivary glands, oral mucosa and hard dental tissues. Therefore, the use of local treatment in the oral cavity is important for correction of these changes.Objective — to improve the properties of oral fluid in patients with chronic obstructive pulmonary disease (COPD) comorbid with coronary heart disease (CHD) with the usage of local therapy in the oral cavity.Materials and methods. The clinical dental study has been performed with involvement of 25 patients in the main group and 24 patients of the observation group, administered the basic therapy for COPD comorbid with CHD. The examinations have been performed for the physical properties of oral fluid (salivation rate, viscosity and pH) at the first visit and 6 months after beginning of local oral therapy.Results and discussion. The comparison of complaints and physical parameters of oral fluid in the beginning of the study and 6 months later demonstrated the decrease of dental complaints frequency, significant increase of salivation, and decrease of oral fluid viscosity in patients who adhered the prescribed therapy. These results demonstrate the high efficacy of local treatment program for the oral cavity. Conclusions. The medicines-induced pathomorphosis, caused by the simultaneous use of several drugs with xerogenic effects, proved to be a very significant factor of changes in the oral fluid of the examined patients.

THE STATE OF HYGIENE AND LOCAL IMMUNITY OF THE ORAL CAVITY IN PATIENTS WITH ANOREXIA NERVOSA

&#x0D; &#x0D; &#x0D; &#x0D; Aim: to study the state of local immunity and oral hygiene in patients with GP suffering from AN.Materials and methods: 35 patients with AN were examined at the age of 19-35 years. In the study we used immunological methods, an index assessment of the state of oral hygiene (PMA, API, GI).Results and discussion: in patients of I group poor oral hygiene was established. The amount of lysozyme in the oral fluid in I group was 0.014 ± 0.07 g/l. A decrease SIgA (11SIgA) was established in patients of I group: I, II and control (135.31 ± 23.17, 130.26 ± 24.21 and 300.34 ± 27.38 mg/l, respectively). A tendency to an increase in the level of IgG in the oral fluid in patients of group II compared with I and the control group (5.35 ± 0.36, vs. 4.57 ± 0.04 and 4.98 ± 0.23 g/l, respectively) was established.Conclusions: poor oral hygiene is an objective indicator which reflects low motivation to maintain oral health. The observed immunodeficiency (11SIgA) in patients with GP is a mirror image of changes occurring in the body of patients with AN.&#x0D; &#x0D; &#x0D; &#x0D;

THE STUDY OF MINERAL COMPONENTS OF THE ORAL FLUID IN PATIENTS WITH DIABETES MELLITUS 1ST TYPE

Oral fluid is an important biological environment that provides a normal functional state of organs and tissues of the oral cavi-ty, due to its multi-component biochemical composition, the vio-lation of which leads to the development of dental pathology. The composition and properties of oral fluid are closely related to metabolic processes in the body, as well as the level of oral hygiene and dental diseases.Purpose of research. To study changes in the mineral composi-tion of oral fluid in patients with type 1 diabetes.Material and methods of research. The collection of non-stimulated mixed saliva (4 ml) in the morning on an empty stomach in graduated tubes in 34 patients with type 1 diabetes aged 18 to 34 years. The control group consisted of 33 people of the appropriate age without General somatic pathology. A col-orimetric method was investigated, the concentration of total calcium with arsenazo III, the concentration of magnesium with silidianin blue. The concentration of inorganic phosphorus was determined by direct reaction with phosphomolybdate. The de-termination was carried out on an automatic biochemical ana-lyzer. The content of ionized calcium, potassium, sodium, pH of the oral fluid was studied on an ion-selective electrolyte analyz-er.Research result. In patients with type 1 diabetes mellitus, signif-icant violations of the mineral composition of the oral fluid were revealed. It is established that in the oral fluid of patients sig-nificantly reduced the content of total and ionized calcium in comparison with indicators of control group patients and the physiological norm, in that time, as the content of inorganic phosphorus in the patients was at the level of individuals of the control group and in the normal range, resulting in the calcium-phosphorus ratio in patients with diabetes mellitus of the 1st type was 2 times lower. The pH value of oral fluid in patients with diabetes mellitus and in the control group did not differ.Summary Thus, in the examined persons with type 1 diabetes mellitus, significant changes in the main mineral components of the oral fluid were revealed, first of all, a reduced content of to-tal and ionized calcium, as a result of which the calcium-phosphorus coefficient decreases, which, of course, worsens the mineralizing function of the oral fluid and reduces the possibil-ity of mineralization of tooth enamel and its restitution in the event of acid demineralization.

THE STUDY OF ORAL FLUID DYNAMIC PARAMETERS ON THE BACKGROUND OF PATHOLOGICAL AND PHYSIOLOGICAL DENTAL ABRASION

Introduction: Violation of oral fluid mineralization processes, which is determined by the mineralization potential of saliva, is associated with changes in the physicochemical parameters of the oral fluid, particularly its viscosity. The aim of our study was to study mineralization potential and types of microstallation of oral fluid as one of the factors of influence on the cariesogenic situation in the oral cavity of patients with physiological or pathological tooth abrasion. Materials and methods: During the examination of patients' oral cavity, a comprehensive assessment of tooth hard tissues was performed in order to of study activity and prevalence of the processes occurring in them. Results: Assessing the physico-chemical parameters of oral fluid in patients of the first experimental group, we obtained the following results: the viscosity of saliva in subgroups was 2,17 ± 0,87 for subgroup #1, 1,78 ± 0,57 for subgroup #2, and 2,15 ± 0,86 for #3 subgroups, which did not have a significant difference between the indices within the group. During the research, the number of independent structures of oral fluid in subgroups 1-3 was 1.67 ± 0.86, 1.67 ± 0.77 and 1.57 ± 0.85. Conclusions: Thus, we have established that the mineralizing function of saliva changes when the cariesogenic situation in the oral cavity arises,. This leads to destabilization of the crystalline structure of the oral fluid and indicates the relationship between its structural and mineralizing properties.

Dynamics of markers of bone resorption in oral fluid in patients with generalized parodontitis during its treatment

Dynamics of markers of bone resorption in oral fluid in patients with generalized parodontitis during its treatment

Determination of Autoantibodies to Salivary Gland Antigens.

Primary Sjögren's syndrome (pSS) is a systemic autoimmune disease marked by dry mouth/dry eye symptoms as a result of the destruction of the salivary and lacrimal glands. Patients with pSS may be at risk for the development of mucosa-associated lymphoid tissue (MALT) lymphoma. Testing of autoantibodies in the oral fluid of patients with pSS or MALT lymphoma using immunoassays may lead to a simple and noninvasive clinical tool for diagnostic or prognostic applications. In this chapter, we describe the procedures and protocols for determining autoantibodies to salivary gland antigens with proteomics-related methods, including 2D gel electrophoresis, liquid chromatography-tandem mass spectrometry (LC-MS/MS), Western blotting, protein microarray, and enzyme-linked immunosorbent assay (ELISA).

The electrolyte composition of the oral fluid in patients with periodontal diseases depending on the psychophysiological state

The electrolyte composition of the oral fluid in patients with periodontal diseases depending on the psychophysiological state

THE FUNCTIONING FEATURES OF PROOXIDANT-ANTIOXIDANT SYSTEM AND IMMUNE PROTECTION DURING DENTAL CARIES TREATMENT IN CHILDREN WITH NEUROPSYCHIATRIC DISORDERS

Aim. To study the functioning of the prooxidant-antioxidant system and immune defense in the treatment of dental caries in children with psychoneurological disorders in different age groups. Materials and methods. Biological material for the study was oral fluid of patients with tooth decay, with neuropsychiatric disorders (n=84 children) and without comorbid pathology (n=47 children), the control group was 45 people. An evaluation of the severity of the disturbances in the prooxidant-antioxidant system in the oral fluid was carried out by determining the intensity of chemiluminescence in the maximum and the area of the outbreak, as well as the overall antioxidant activity characterizing the state of the antioxidant system. Among the indices of the enzyme link of antioxidant protection, the activity of superoxide dismutase, catalase was determined, and in addition, the integral index of the functioning of antiradical protection enzymes was calculated. Investigation of the state of local immune defense was carried out by determining the content of proinflammatory and pro-inflammatory interleukins, and also assessing the level of lactoferrin. Conclusion. The functioning of the prooxidant-antioxidant system and the regulation of immune defense in children with psychoneurological disorders in different age groups have a number of characteristics with which the clinical indices correlate. The use of complex dental care ("Multistep" regulations) allows more complete rehabilitation of patients and achieving significantly better results in normalizing the biochemical state of the oral fluid.