Phnom Penh, Cambodia, is one of the most rapidly urbanizing cities in the world, and this urbanization is driving an increase in the urban heat island (UHI) effect. The UHI effect is the experience of urban areas getting higher temperatures than rural areas due to the concentration of heat-absorbing materials such as concrete and asphalt. This study analyzed air temperature, wind speed and direction data from Phnom Penh in 2021 to investigate the UHI effect and its impact on human thermal discomfort. The study found that the UHI intensity is stronger in the central business district (CBD) of Khan Boeung Keng Kang than in the suburban district of Khan Pou Senchey. This is likely due to the higher built-up area and lower wind speed in Khan Boeung Keng Kang. The study also found that the UHI intensity is stronger during the dry season than during the wet season. This is likely due to the lower humidity during the dry season, which allows surfaces to absorb and retain more heat. The increased UHI intensity in Phnom Penh can create significant human thermal discomfort, especially during the daytime. This discomfort can lead to a variety of health problems, including heat cramps, heat exhaustion, and heat stroke. The study concludes that it is important to take steps to mitigate the UHI effect in Phnom Penh and other rapidly urbanizing cities. This could include measures such as increasing green space, using lighter-colored building materials, and promoting sustainable transportation options. The results of this study can enhance more understanding of the UHI effects and have practical implications on the appropriate UHI-related mitigation and adaptation measures.