Optimal Extraction Research Articles

Utilizing ultrasound for the extraction of polysaccharides from the tuber of Typhonium giganteum Engl.: Extraction conditions, structural characterization and bioactivities.

Polysaccharides from the dried tuber of Typhonium giganteum Engl. (TGEPs) were obtained by utilizing ultrasonic-assisted extraction (UAE) as the extraction method. The determination of optimal process parameters for the UAE of TGEPs (TGEP-U) was accomplished through the application of response surface methodology (RSM). The structural characteristics, antioxidant and hypoglycemic effects of TGEP-U and TGEPs obtained by hot water extraction (TGEP-H) were then compared. Consequently, the optimum extraction conditions predicted by RSM for TGEP-U were obtained as adding water at a ratio of 31mL/g and extracted for 32min under an ultrasound power of 440W. In the verification experiment, the actual yield of TGEP-U was 7.32±0.18%. It was found that UAE could increase the yield and the total sugar content of TGEPs. Meanwhile, chemical composition analysis showed that both TGEP-U and TGEP-H were mainly composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose, but the monosaccharide molar ratios were changed by UAE. Analysis of molecular weight (Mw) revealed the presence of three primary constituents within TGEP-U, and four main components in TGEP-H, and UAE reduced the average Mw of TGEPs. No obvious difference was found in the Fourier transform infrared spectroscopy analysis of TGEP-U and TGEP-H. The Congo red and Circular dichroism tests demonstrated that TGEP-U and TGEP-H had non-three helical structure. Scanning electron microscope observation further revealed that the aggregation of functional groups within TGEPs may be influenced by ultrasound, thereby affecting their powder morphology. TGEP-U has slightly poorer thermal stability than TGEP-H, which may be affected by ultrasonic cavitation effects. The results also indicated that TGEP-U had better antioxidant and hypoglycemic activity than TGEP-H. In summary, UAE is an effective method to extract and enhance the activity of TGEPs with enormous research value and potentials.

Optimization of ultrasonic-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharides from Eucommia ulmoides.

For the valorization of Eucommia ulmoides (EU) for the functional food industry, the process of ultrasonic-assisted deep eutectic solvent (DES) extraction of EU polysaccharides (EUP) was optimized by response surface methodology. After response surface analysis and experimental verification, the optimum extraction conditions were as follows: the molar ratio of choline chloride to oxalic acid was 0.9:1, the water content of DES was 40.28%, the ratio of material to liquid was 1:22 g/mL, the time was 90 min, and the power was 320 W. The extraction rate of EUP was 1.71%, which was higher than the extraction rate of 0.75% by ultrasonic water extraction under the same conditions, and the optimization effect was better. After DEAE column gradient elution and Sephadex G-75 gel column chromatography, the elution curve and a refined polysaccharide (EUP-1) were obtained. The IC50 values of EUP-1 against α-glucosidase and α-amylase were 0.091 and 0.011 mg/mL. The IC50 values of EUP-1 on DPPH and ABTS·+ scavenging were 0.065 and 0.065 mg/mL. The monosaccharide composition of EUP-1 was analyzed as mannose, rhamnose, glucuronic acid, and galacturonic acid in the following molar ratio: 1.00: 1.28: 1.53: 1.88.

Switchable hydrophilicity solvent/water emulsion-based extraction of polyphenols from grape skin

In this present study, switchable hydrophilicity solvent (SHS) was dispersed into water to induce the SHS/water emulsion for the ultrasound-assisted extraction (UAE) and enrichment of polyphenols from grape skin. The single factor experiment design, orthogonal experiment design and Response Surface Methodology (RSM) coupled with Box-Behnken Design (BBD) were successively applied to optimize the extraction. The optimal extraction conditions were as follows: SHS of N,N-Dimethylcyclohexylamine (DMCHA), DMCHA/water volume ratio of 1:1, liquid-solid ratio of 20 mL/g, ultrasound time of 24.93 min, CO2 bubbling time of 120 min and CO2 bubbling rate of 0.25 L/min. Compared to the ethanol-based UAE, the optimal DMCHA/water emulsion-based UAE not only gave a higher extraction yield (14.07%, 49.84% enhanced), but also exhibited the content differences of mono-phenols. Furthermore, the DMCHA/water emulsion-based UAE showed stronger antioxidant activity than that of the ethanol-based UAE. In addition to the low economic cost, low reagent residues and high DMCHA recovery, this proposed extraction method has been proved to be a promising alternative for industrial extraction.

Optimizing ultrasonic extraction of polysaccharides from Spirulina platensis and evaluating their antioxidant and antibacterial activities in acidic environments.

In this study, the response surface method was used to optimize the ultrasonic extraction of polysaccharide from Spirulina platensis. The optimal extraction conditions were ultrasonic time 50 min, solid-liquid ratio 1: 40, ultrasonic power 265 w, and the predicted extraction rate was 6.861 ± 0.116 %. The FT-IR and NMR confirmed that polysaccharide from S. platensis would protonate to form β-glucoside bond in acidic environment, and the original carbonyl group would be converted to carboxyl group and then esterified to form acyl group donor. In the acidic pH range (7.0, 6.0, 5.0 and 4.0), the antioxidant, nitrite scavenging and bacteriostatic activities of the extracted polysaccharide from S. platensis were measured in vitro. The results showed that polysaccharide of S. platensis had better antioxidant activity in acidic environment and better performance in low pH condition. In addition, it has the best inhibitory effect on Salmonella enteritidis (14.3-17.15 mm) in acidic environment, and the inhibitory effect on Staphylococcus aureus (11.95-12.87 mm) is stronger than that in neutral environment (10.48-12.08 mm). To sum up, the structure of polysaccharide from S. platensis will change in acidic environment, and it has good antioxidant and antibacterial properties.

Accumulation, extraction, purification, and antioxidants characteristics of proanthocyanidins from Chinese dwarf cherry (Cerasus humilis)

Background Cerasus humilis is rich in proanthocyanidins (PAs), which confer antioxidant activity. However, little is known about PAs in C. humilis fruits. Objective This study aimed to analyze the accumulation characteristics, extraction, purification, and antioxidant activities of C. humilis PAs. Methods The total proanthocyanidins content (TPAC) and total antioxidant capacity (TAOC) of fruits of three C. humilis varieties (Jing-Ou 1, Jing-Ou 2, and Nong-Da 4) were determined. PA extraction was optimized using an orthogonal experimental design. The purification process of macroporous resin method was optimized and the purified PAs of C. humilis fruit was further refined by gelatin precipitation methods. The antioxidant capacity of C. humilis PAs before and after purification was by 2,2-biphenyl-1-picrylhydrazyl (DPPH) assay and 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay in vitro. Results TPAC and TAOC showed a positive correlation and decreased in all three varieties as fruits ripened . After ripening, Jing-Ou 2 had the highest PAs content. The optimal solvent extraction method yielded a TPAC of 883.0 mg/100 g FW. The purity of PAs reached 47.52% using the macroporous resin method and 98.14% using gelatin precipitation. IC50 values of the purified PAs were comparable to those of vitamin C at the same mass concentration. Conclusions In this study, C. humilis PAs were systematically investigated for the first time. Not only its dynamic changes during fruit development were analyzed, but also the extraction and purification methods were optimized. Meanwhile, its antioxidant activity was verified, which provided a theoretical basis for the development and utilization of C. humilis PAs as antioxidants in functional foods and pharmaceutical formulations and sheds light on the health benefits of the species.

Naringin vs. Citrus x paradisi L. Peel Extract: An In Vivo Journey into Oxidative Stress Modulation

Citrus fruits, mainly grapefruit (Citrus x Paradisi L.), are rich in bioactive compounds with potential antioxidant properties. This study investigated the antioxidant effects of naringin (NR) and ethanolic Citrus x paradisi L peel (E) in reducing aluminum chloride (AlCl3)-induced oxidative stress in mice. Quantitative analysis using HPLC identified optimal extraction conditions, combination ultrasound and reflux extraction (UH50), resulting in high concentrations of naringin (49.13 mg/g) and naringenin (63.99 µg/g). Mice were treated with NR and E to evaluate their effects on key markers of oxidative stress: reduced glutathione (GSH), malondialdehyde (MDA), and catalase (CAT). The E effectively reduced MDA levels in blood, brain, and liver tissues, with a more substantial effect on controlling lipid peroxidation. In contrast, NR was more effective in restoring GSH levels and CAT activity, suggesting a broader enhancement of antioxidant defense. These findings provide information about specific mechanisms of NR and E and their therapeutic potential in managing oxidative stress and developing products with synergistic efficacy.

Optimization of Green Ultrasound-Assisted Extraction of Carotenoids and Tocopherol from Tomato Waste Using NADESs

The purpose of this study was to extract the lipophilic fraction from one of the largest source of waste in the industrial sector, namely, the tomato residue from processing the fruit. In order to make this process more environmentally sustainable, this study used a green extraction protocol employing natural deep eutectic solvents (NADESs) combined with a less energy-consuming technology, the ultrasound-assisted extraction (UAE) method, to simultaneously recover carotenoids and tocopherol from dried powder tomato waste. Two NADESs, one hydrophilic and one hydrophobic, were prepared and compared to support high extraction efficiency and increase the stability of the extracted compounds. The optimal extraction parameters were identified as choline chloride:1,3-butanediol (1:5)-based NADES, a solid-to-liquid ratio of 1:20 (w/v), time of extraction 12 min, temperature 65 °C, radiation frequency 37 Hz, and an ultrasound power level of 70%. The extraction process was intensified and resulted in extracts rich in lycopene (215.13 ± 4.31 μg/g DW), β-carotene (206.95 ± 3.27 μg/g DW), and tocopherol (130.86 ± 8.97 μg/g DW) content, with the highest antioxidant capacity 93.84 ± 0.18 mM Trolox equivalent. Incorporating NADESs for the extraction of bioactive compounds offers numerous benefits, such as improved sustainability, enhanced extraction efficiency, better protection of sensitive compounds, and reduced environmental impact. These advantages make NADESs a promising alternative to traditional organic solvents, especially in industries that require natural, green, and efficient extraction processes for valuable bioactive molecules.

Optimizing Quercetin Extraction from Taraxacum mongolicum Using Ionic Liquid–Enzyme Systems and Network Pharmacology Analysis

Quercetin in Taraxacum mongolicum was extracted by ultrasound-assisted extraction in synergy with an ionic liquid–enzyme complex system, and the antioxidant function of quercetin was investigated based on network pharmacology. From 1-butyl-3-methylimidazolium chloride, 1-butyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium tetrafluoroborate, 1-butyl-3-methylimidazolium bromide, and 1-butyl-3-methylimidazolium tetrafluoroborate, the first step was to choose the appropriate ionic liquid. Subsequently, a response surface methodology and single-factor experiment were used to optimize the extraction process. The quercetin and the key targets for antioxidants were obtained from a public database. Antioxidant activity was assessed by measuring the scavenging rate of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and hydroxyl radicals(•OH). The approach revealed that the optimal extraction process was the liquid–solid ratio of 31.62:1 mL/g, enzymatic temperature of 55 °C, and the amount of cellulase added was 14.79% of the dry weight of dandelion. Under this condition, the yield of quercetin was 0.24 ± 0.011 mg/g, which was 1.3 times higher than that of the conventional reflux extraction method of 0.185 ± 0.015 mg/g. Pharmacological findings showed 57 cross-targets of quercetin with antioxidants. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that antioxidant function may be related to chemical carcinogenesis-reactive oxygen species, and the Phosphoinositide 3-kinase/protein kinase B signaling pathway. Quercetin has strong DPPH and •OH radical scavenging activity. The development and use of industrial dandelion are supported by this sustainable and effective method of extracting quercetin from dandelion.

Efficacy Evaluation of Jania rubens Extract Against Four Pathogenic Strains Associated with Foodborne Disease in Iran

This study evaluated the antimicrobial properties of Jania rubens algae extract, obtained via Soxhlet extraction, against food spoilage and pathogenic bacteria. Using response surface methodology and the Box-Behnken design, the research optimized extraction parameters, including solvent type, solvent-to-algae ratio, and extraction duration. Antimicrobial efficacy was assessed through the zone of inhibition (ZOI), Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) against Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, and Bacillus cereus. Results showed MIC and MBC values for E. coli ranged from 0.32–1.5 and 2–3, respectively; for S. enteritidis, 0.98–1.9 and 2.5–3.5; for S. aureus, 0.4–1.5 and 0.8–2.8; and for B. cereus, 0.36–1.35 and 0.85–1.85. Optimal extraction conditions included acetone as the solvent, a 5:1 solvent-to-algae ratio, and extraction durations of 2 or 6 hours. The algae extract significantly extended the lag phase and reduced bacterial growth rates, with effects intensifying at higher concentrations. The study concludes that Jania rubens extract, particularly under optimized Soxhlet extraction conditions, exhibits potent antibacterial activity, making it a promising natural antimicrobial agent for food preservation.

Efficacy Evaluation of Jania rubens Extract Against Four Pathogenic Strains Associated with Foodborne Disease in Iran

This study evaluated the antimicrobial properties of Jania rubens algae extract, obtained via Soxhlet extraction, against food spoilage and pathogenic bacteria. Using response surface methodology and the Box-Behnken design, the research optimized extraction parameters, including solvent type, solvent-to-algae ratio, and extraction duration. Antimicrobial efficacy was assessed through the zone of inhibition (ZOI), Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) against Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, and Bacillus cereus. Results showed MIC and MBC values for E. coli ranged from 0.32–1.5 and 2–3, respectively; for S. enteritidis, 0.98–1.9 and 2.5–3.5; for S. aureus, 0.4–1.5 and 0.8–2.8; and for B. cereus, 0.36–1.35 and 0.85–1.85. Optimal extraction conditions included acetone as the solvent, a 5:1 solvent-to-algae ratio, and extraction durations of 2 or 6 hours. The algae extract significantly extended the lag phase and reduced bacterial growth rates, with effects intensifying at higher concentrations. The study concludes that Jania rubens extract, particularly under optimized Soxhlet extraction conditions, exhibits potent antibacterial activity, making it a promising natural antimicrobial agent for food preservation.

Efficacy comparison of optimal natural orifice specimen extraction for robotic middle rectal cancer resection in women: transanal or transvaginal orifice

PurposeThis study aimed to determine the optimal natural orifice specimen extraction (NOSE) method for robotic-assisted mid-rectal cancer resection in women.MethodsThis retrospective propensity score-matched (PSM) study was to analyze the clinical data prospectively collected from female rectal cancer patients who underwent either robotic-assisted transvaginal specimen extraction (RATV) or robotic-assisted transanal specimen extraction (RATA) at our center between June 2016 and December 2022. The main outcome measures were urinary, anal, and sexual function. Disease-free survival (DFS), and overall survival (OS) were also included .ResultsAnal function, assessed by the Wexner score, was better in the RATV group than in the RATA group (P = 0.034). Additionally, pre-menopausal women in RATV group exhibited superior anal function over those in RATA group (P = 0.031). There was no statistically significant difference in urinary function between the groups for both pre-menopausal and peri-menopausal patients (P = 0.711, P = 0.106). No difference was observed in sexual function between the two groups (P = 0.351); however, pre-menopausal patients in RATA group had better sexual function than those in RATV group (P = 0.045). Univariate logistic regression analysis showed surgical procedure was not a significant factor for the occurrence of sexual dysfunction. There were no significant difference in DFS (P = 0.845)and OS (P = 0.642) between the two groups.ConclusionThough the postoperative efficacy of the RATA and RATV was equivalent on urinary and sexual function, RATV is an optimal natural orifice specimen extraction for robotic middle rectal cancer resection in women based on anal function.

Sustainable Supercritical Carbon Dioxide Extraction of Value-Added Lignan from Sesame Meal: Achieving Green Neuroprotection and Waste Valorization by Optimizing Temperature, Solvent, and Pressure

In pursuing sustainable health solutions and growing demand for neuroprotective interventions, the industry demands alternative green extraction technologies to valorize agri-food by-products. This study aimed to develop an optimized supercritical carbon dioxide extraction to isolate sesame meal’s functional compound (lignans) and assess their neuroprotective effects. Extraction was performed at various pressures (2–4 kpsi), temperatures (40–60 °C), co-solvent concentrations (2–25 mol% ethanol), and CO2 collection segments (0–100 NL) to systematically analyze extraction parameters. Extracts were analyzed quantitatively using high-performance liquid chromatography followed by neuroprotective mechanisms analysis through PC12 neural cell and ischemic stroke models. The results showed that adding ethanol enhanced the polarity and density of supercritical CO2, improving the extraction efficiency of polar lignans. Optimal extraction conditions (4 kpsi, 50 °C, 10 mol% ethanol) yielded the highest sesamol, sesamin, and sesamolin. Extracts showed remarkable protective capabilities when subjected to oxygen–glucose deprivation (OGD) conditions simulating ischemic stress, preventing the enhancement of lactate dehydrogenase activity. Relatively low extract concentrations (25–100 μg/mL) significantly mitigated cellular damage induced by short and extended OGD conditions. The findings revealed green extraction methodologies’ capability to transform sesame meal, a food processing waste, into value-added compounds, in line with sustainable development goals for responsible and sustainable food production, particularly SDGs 3, 9, 12, and 13.

Carbon nanotubes stabilized in chitosan sponge (CNT-CS) as a new alternative clean-up sorbent for pesticide multiresidue determination in eggplants using QuEChERS and UHPLC-MS/MS.

The continuous development and application of pesticides in agriculture require robust multiresidue detection methods to guarantee food safety. This study introduces a novel method for multiresidue determination of pesticides in eggplants using the QuEChERS procedure, incorporating a clean-up step using carbon nanotubes stabilized in chitosan sponge (CNT-CS) and ultra-high performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) for analysis. Upon identifying the optimal extraction conditions, various sorbents were assessed for their efficacy in the dispersive solid-phase extraction (d-SPE). Among these, the biosorbent CNT-CS emerged as the most efficient and cost-effective material, showing the best recovery results from spiked blank samples. The validation demonstrated that the method was adequate for analyzing 111 pesticides in eggplant samples with practical method quantification limits from 5 to 25µgkg-1. When applied to commercial eggplant samples, 27 pesticides were detected, demonstrating the importance of the proposed method. It is noteworthy that the conducted work represents the first report on the use of the biosorbent CNT-CS in the clean-up step of the QuEChERS method. The results obtained in the validation and application steps demonstrate that the proposed method is an excellent alternative for monitoring programs.

Synthesis of a fluorophilic magnetic microporous organic network for selective enrichment of fipronil and ethiprole in milk and egg samples.

Considering the widespreadly use, large consumption, and serious environmental and health threats of phenylpyrazole insecticides (PPIs), development of a selective and sensitive method for accurate detection of their residuals in food samples is of great significance and challenging. Herein, depending on the hydrophobic and F-containing characteristics of PPIs, a novel fluorinated magnetic microporous organic network (FMMON) was designed and prepared for efficient and selective magnetic solid-phase extraction (MSPE) of two typical PPIs (fipronil and ethiprole) from milk and egg samples before the HPLC-UV determination. FMMON owned large specific surface area, multiple interaction sites, excellent magnetic separation performance and stability and exhibited good extraction and selectivity for fipronil and ethiprole through the specific F-F, hydrogen bonding, hydrophobic, and π-π interactions. Under optimal extraction conditions, the established FMMON-MSPE-HPLC-UV method provided wide linear ranges (0.1-1000 µg L-1), low limits of detection (0.03-0.05 µg L-1), large enrichment factors (96.2-99.7), low adsorbent consumption (4 mg), and short extraction time (6 min) for fipronil and ethiprole. This work revealed the bright future of fluorinated MONs in sample pretreatment of fluorinated contaminants in complex substrates.

Composition and antioxidant activity of flavonoids from two different species of Amomi Fructus extracted using natural deep eutectic solvents.

Amomi Fructus, a mature fruit from a ginger family plant, has various species, resulting in inconsistent sourcing and quality. Most studies distinguish species by volatile compounds, yet research shows it also contains flavonoids with notable pharmacological effects. Solely focusing on volatile compounds could lead to considerable resource waste. This study aims to establish flavonoid markers in Amomi Fructus to distinguish its species, assess quality, and promote efficient resource use. Utilizing natural deep eutectic solvents (NADES) and response surface methodology (RSM), an optimal extraction system (choline chloride-ethylene glycol) yielded 41.38mg RE/g total flavonoids. LC-MS analysis of 18 Amomi Fructus batches identified 26 flavonoids, quantified 19, and highlighted three key markers-epicatechin, procyanidin B2, and procyanidin B4-that effectively differentiate Amomum villosum Lour. (AMV) from Amomum villosum Lour. var. xanthioides T.L. Wu et Senjen (AMVX). Finally, flow cytometry confirmed these markers' antioxidant activity, effectively reducing H₂O₂-induced oxidative damage in GES-1 cells.

Simultaneous extraction of flavonoids and saponins from functional food via basic deep eutectic solvent-assisted mechanochemical extraction.

An efficient, green, and novel basic deep eutectic solvent (DES)-assisted mechanochemical extraction method, combined with ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, is proposed for simultaneous extraction and determination of target compounds from functional food. Several parameters are investigated using single-factor optimization, and response surface methodology is employed to determine the optimum extraction conditions. The conditions include a grinding time of 316.29s, 83.72μL of DES (K2CO3 and glycerol), and vortex time of 2.408min. The developed method provides good linearity (R2>0.9958), stable intraday and interday precision ranging from 0.07% to 3.31%, high sensitivity (LODs <20.83ng/mL), and acceptable accuracy with recoveries of 83.75%-102.02%. Moreover, satisfactory greenness (0.71) and practicality (72.5) are achieved in terms of the Analytical Greenness metric and the blue applicability grade index. The proposed method notably contributes to the simultaneous extraction and detection of flavonoids and saponins in astragali radix.

Boosting Therapeutic Effect of Turmeric, Coffee, and Chili Extracts Through Experimental Design and Encapsulation as Nanostructured Lipid Carriers for Novel Heath Supplements.

This study investigates the potential synergistic effects of extracts from Curcuma longa (turmeric), Coffea arabica (Arabica coffee beans), and Capsicum annuum (chili peppers) in reducing oxidative stress and inflammation, which are associated with metabolic disorders such as obesity, diabetes, and cardiovascular diseases. Using a systematic design of experiment (DoE) optimization approach, an optimal extract ratio of 1:3:4 (turmeric: coffee: chili) was identified. The efficacy of the extract combination was assessed through various antioxidant assays, inhibition of inflammation-related gene expression, and safety testing via the 3-(4,5-dimethylthazolk-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The extract combination showed higher antioxidant activity and comparable anti-inflammatory effects relative to each single extract. Additionally, the extract combination demonstrated effective activity compared with turmeric extract while using a lower concentration, resulting in reduced cytotoxicity. The optimized extract combination was successfully incorporated into nanostructured lipid carriers (NLCs) with a hydrodynamic diameter of 258.0 ± 10.2 nm, which effectively redisperses after the spray-drying process with increased diameter to 349.8 ± 49.6 nm. Under stress conditions, the stability of curcumin and capsaicin in dried-NLCs was maintained. In summary, the optimized extract-loaded NLCs formulation, achieved through a multistage approach, shows promise in mitigating oxidative stress and inflammation, suggesting its potential as a valuable daily dietary supplement.

Enhanced Fault Prediction for Synchronous Condensers Using LLM-Optimized Wavelet Packet Transformation

This paper presents an enhanced fault prediction framework for synchronous condensers in UHVDC transmission systems, integrating Large Language Models (LLMs) with optimized Wavelet Packet Transform (WPT) for improved diagnostic accuracy. The framework innovatively employs LLMs to automatically optimize WPT parameters, addressing the limitations of traditional manual parameter selection methods. By incorporating a Multi-Head Attention Gated Recurrent Unit (MHA-GRU) network, the system achieves superior temporal feature learning and fault pattern recognition. Through intelligent parameter optimization and advanced feature extraction, the LLM component intelligently selects optimal wavelet decomposition levels and frequency bands, while the MHA-GRU network processes the extracted features for accurate fault classification. Experimental results on a high-capacity synchronous condenser demonstrate the framework’s effectiveness in detecting rotor, air-gap, and stator faults across diverse operational conditions. The system maintains efficient real-time processing capabilities while significantly reducing false alarm rates compared to conventional methods. This comprehensive approach to fault prediction and diagnosis represents a significant advancement in synchronous condenser fault prediction, offering improved accuracy, reduced processing time, and enhanced reliability for UHVDC transmission system maintenance.

OPTIMIZATION OF THE CONDITIONS OF ULTRASONIC EXTRACTION OF POLYPHENOLIC COMPOUNDS FROM THE INFLORESCENCES OF KOENIGIA WEYRICHII (F. SCHMIDT) T.M. SCHUST. ET REVEAL

Introduction. Inflorescences of Koenigia weyrichii (F. Schmidt) T.M. Schust. et Reveal contains a large amount of flavonoids. However, at present, the most optimal conditions for their extraction by water-ethanol mixtures have not been obtained, and the effect of ultrasound on the extraction pro-cess has not been fully studied. The aim of the study was to optimize the conditions of ultrasonic water-ethanol extraction of secondary metabolites from the inflorescences of K. weyrichii. Material and methods. Optimal extraction conditions have been established using the Box-Behnken algorithm. The total content of polyphenols was determined using the Folin-Ciocalteu method, and flavonoids were determined using a complexation reaction with aluminum chloride. The total antioxi-dant activity was assessed using the phosphomolybdate method. The content of polyphenolic acids was determined by HPLC. The ability of the extract obtained under optimal conditions to inhibit free radicals was determined by DPPH and ABTS methods. Results. The highest yield of the target substances is achieved in 60 minutes of extraction in 50% aqueous ethanol at a temperature of 50 ° C, and mixing plant material with a particle size of 0.1 mm with an solvent in a ratio of 1:10 (mass to volume. It has been shown that ultrasound exposure reduces the yield of target substances. Conclusions. The results of the work are the scientific basis for the development of methods of phytochemical analysis and technologies for the pro-duction of pharmaceuticals based on the studied plant.

Optimization of Ultrasound-Assisted Extraction (UAE) of Phenolics from Blueberries by Response Surface Methodology (RSM)

Blueberries are rich in polyphenols, vitamins, and minerals, enhancing their health benefits. Extracting bioactive compounds from blueberries is vital for the pharmaceutical, nutraceutical, and food industries. Ultrasound-assisted extraction (UAE) is an efficient and eco-friendly method for isolating phenolic compounds. This study employed response surface methodology (RSM) to optimize the UAE conditions, specifically focusing on solvent composition, solvent volume, and extraction time, to maximize total polyphenol (TP) and total anthocyanin (TA) content. Blueberry samples were extracted using acidified methanol as the primary solvent in an ultrasonic bath. Optimal extraction conditions were determined using a quadratic Box–Behnken design and analyzed through ANOVA. Results showed that the solvent composition had the most significant effect on TP and TA extraction, followed by solvent volume, while extraction time showed the least influence. The optimized conditions were determined to be methanol solution 99:1 v/v (12.5 mL) and an extraction time of 97.7 min for TP, while for TA, the optimal conditions were 17.9 mL of the same methanol solution with an extraction time of 100.9 min. Obtained with these conditions were 408.2 ± 3.9 mg GAE/100 g FW of TP and 127.4 ± 1.7 mg C3GE/100 g FW of TA. These findings highlight the effectiveness of UAE combined with RSM for the efficient extraction of bioactive compounds from blueberries, providing a framework for industrial-scale applications by an efficient process.