Optical Flow Cell Research Articles

AI-Based Integrated Smart Process Sensor for Emulsion Control in Industrial Application

In industry, reliable process supervision is essential to ensure efficient, safe, and high-quality production. The droplet size distribution represents a critical quality attribute for emulsification processes and should be monitored. For emulsion characterization, image-based analysis methods are well-known but are often performed offline, leading to a time-delayed and error-prone process evaluation. The use of an integrated smart process sensor to characterize the emulsification process over time enables the real-time evaluation of the entire system. The presented integrated smart process sensor consists of an optical measurement flow cell built into a camera system. The overall system is placed in a bypass system of a production plant for emulsification processes. AI-based image evaluation is used in combination with a feature extraction method (You Only Look Once version 4 (YOLOv4) and Hough circle (HC)) to characterize the process over time. The sensor system is installed in the plant and tested with different cosmetic products. Various iteration, prototyping, and test steps for the final sensor design are performed prior to this in a laboratory test setup. The results indicate robust and accurate detection and determination of the droplet size in real time to improve product control and save time. For benchmarking the integrated smart process sensor, the results are compared with common analysis methods using offline samples.

Rapid prototyping of a modular optical flow cell for image-based droplet size measurements in emulsification processes

Emulsification processes are often found in the process industry and their evaluation is crucial for product quality and safety. Numerous methods exist to analyze critical quality attributes (CQA) such as the droplet sizes and droplet size distribution (DSD) of an emulsification process. During the emulsification process, the optical process accessibility may be limited due to high disperse phase content of liquid-liquid systems. To overcome this challenge, a modular, optical measurement flow cell is presented to widen the application window of optical methods in emulsification processes. In this contribution, the channel geometry is subject of optimization to modify the flow characteristics and produce high optical quality. In terms of rapid prototyping, an iterative optimization procedure via SLA-3D printing was used to increase operability. The results demonstrated that the flow cell resulting from the optimization procedure provides a broad observation window for droplet detection.Graphical abstract

Liquid crystal-based label-free low-cost sensing platform: Engineering design based on interfacial interaction and transport phenomena

Typically, the detection methods of chemical species in sensing platform involves the use of labelling agents. Even though it is selective, the process is complex and time consuming. Liquid crystals (LC) are sensitive and rapidly responsive to the presence of any foreign species. When a LC layer interacts with aqueous (ionic surfactant) solution, there is homeotropic alignment of the director orientation due to the anisotropic interactions at the interface. This leads to the dark birefringence patterns observed in polarised microscope under cross polarisation. In the presence of H+ in the aqueous layer, the LC orientation is distorted resulting in a change of the birefringence patterns in the polarised micrographs. We have prepared an optical cell where the aqueous solution can interact with the LC layer confined within micrometre sized grids. The flow arrangement and the associated mass transfer process is optimised for enhanced interaction. The elastic interactions of the LC layer with H+ at the interface distorts the director orientation, and the birefringence pattern is captured with polarised microscope (cross-polarisation). There is a correlation between the polarised micrographs and the concentration of the H+, which forms the basis of detection principle. The detection of H+ has no interference with salts. The present method can be used for recognition of (bio-) chemical reactions releasing H+. The developed optical flow cell is inexpensive, less than $1. The outcome of the present work may be useful for technological adaption and prospective use in point-of-care devices and field test kits.

Monitoring the State of Charge Imbalance of Vanadium Redox Flow Batteries Via Dual Online UV/Visible Spectroscopy

Electrolyte imbalance in Redox Flow Batteries (RFBs) is known to result in significant capacity decay over cycling [1]. This drawback is inherent to the flow battery technology and is caused by unwanted physico-chemical phenomena, such as species and water crossover, hydrogen evolution, air oxidation and precipitation of vanadium ions. The time evolution of electrolyte imbalance is often disregarded or underestimated when measuring the open-circuit voltage (OCV) for keeping track of the battery State of Charge (SOC). In order to correct such asymmetry, in operando real-time monitoring of the SOC of the positive and negative electrolytes would be highly desirable.In this work, we present an experimental test bench for the continuous online monitoring of the anolyte and catholyte SOCs via UV/visible spectroscopy during cycling of an all-vanadium redox flow battery (VRFB). The system notably includes 3D printed microfluidic optical flow cells, an optical switch, and an in-house microfluidic electrochemical cell. Key steps for the calibration of UV/visible spectroscopy with vanadium are presented and applied independently to both electrolytes to accurately estimate the SOC while taking into account the total vanadium concentration [2]. An experimental campaign is then carried out under varying conditions (initial average oxidation state (AOS), air oxidation, crossover, etc.) to illustrate the real-time evolution of electrolyte imbalance during VRFB battery cycling. The results suggest that the imbalance may arise from asymmetric initial Average Oxidation State (AOS ≠ 3.5) [3], vanadium crossover and also hydrogen evolution, which notably reduces the charging speed of the negative electrolyte [4]. The results highlight the benefits of UV/Visible spectroscopy to obtain SOC data for any of the three stable vanadium electrolytes: i) negative, VII/VIII ii) positive VIV/VV and even the iii) VIII/VIV pair. Moreover, the measurements are online, non-invasive and could provide real-time data to a battery management system either in conventional or microfluidic VRFBs to correct the SOC imbalance and maintain the battery capacity and efficiency [1]. Acknowledgments This work has been partially funded by FEDER/Ministerio de Ciencia, Innovación y Universidades – Agencia Estatal de Investigación Project PID2019-106740RB-I00, and by Grant IND2019/AMB-17273 of the Comunidad de Madrid. A. A. Maurice Energy acknowledges the support of an MCSF-Cofund “Energy for Future” (E4F) postdoctoral research fellowship by the Spanish Iberdrola Foundation (GA-101034297).

Inexpensive spectrophotometric determination of nitrite with a laboratory-constructed flow cell

Nitrite is a carcinogenic substance and its measurement in water samples is important to protect mankind. Here we have introduced a simple and inexpensive fluidic system for the online determination of nitrite in water samples. For this purpose, a highly sensitive, optical flow cell detector was fabricated and used in a fluidic system to prepare a compact unit for nitrite monitoring. Safranin solution in acidic media was used in the system for the sensitive and selective determination of nitrite. In acidic media, nitrite reacted with safranin to form a diazonium salt which caused the dye in the solution to change from reddish-orange to blue. The safranin solution absorbance, which is related to nitrite concentration, was monitored by the fabricated photometer with the flow detector at 525 nm. Water samples were processed to evaluate the effectiveness of both the introduced system and the analytical procedure. Good analytical performance was obtained, including a linear response from 0.3 up to 3 mg/l nitrite (r = 0.9918) with a detection limit of 0.1 mg/l and a relative standard deviation of 4.24% (n = 10), using the proposed setup.

Nondispersive Infrared Gas Analyzer for Partial Pressure Measurements of a Tantalum Alkylamide During Vapor Deposition Processes.

A nondispersive infrared gas analyzer was demonstrated for investigating metal alkylamide precursor delivery for microelectronics vapor deposition processes. The nondispersive infrared analyzer was designed to simultaneously measure the partial pressure of pentakis(dimethylamido) tantalum, a metal precursor employed in high volume manufacturing vapor deposition processes to deposit tantalum nitride, and dimethylamine, the primary decomposition product of pentakis(dimethylamido) tantalum at typical delivery conditions for these applications. This sensor was based on direct absorption of pentakis(dimethylamido) tantalum and dimethylamine in the fingerprint spectral region. The nondispersive infrared analyzer optical response was calibrated by measuring absorbance as a function of dimethylamine and pentakis(dimethylamido) tantalum density. The difference between the mass of material removed from the ampoule during flow tests as measured gravimetrically and as determined optically, by calculating flow rates from the nondispersive infrared analyzer measurements, was only ≈2 %. The minimum detectable molecular densities for pentakis(dimethylamido) tantalum and dimethylamine were ≈2 × 1013 cm-3 and ≈5 × 1014 cm-3, respectively (with no signal averaging and for a sampling rate of 200 Hz), and the corresponding partial pressures were ≈0.1 Pa and ≈2 Pa for pentakis(dimethylamido) tantalum and dimethylamine, respectively (for an optical flow cell temperature of 93 ℃). Pentakis(dimethylamido) tantalum could be detected at all conditions of this investigation and likely the majority of conditions relevant to high volume manufacturing tantalum nitride deposition. Dimethylamine was not detected at all conditions in this study, because of a lower nondispersive infrared analyzer sensitivity to dimethylamine compared to pentakis(dimethylamido) tantalum and because conditions of this study were selected to minimize DMA production. While this nondispersive infrared gas analyzer was specifically developed for pentakis(dimethylamido) tantalum and dimethylamine, it is suitable for characterizing the vapor delivery of other metal alkylamide precursors and the corresponding amine decomposition products, although in the case of some metal alkylamides a different bandpass filter would be required.

Soluble reactive phosphorous determination in wastewater treatment plants by automatic microanalyzers

The analysis of soluble reactive phosphate (SRP) in water is key to control water quality. In order to continuous monitor orthophosphate content in water during treatment processes and in the effluents of wastewater treatment plants, conventional procedures, usually performed in a laboratory, must be adapted. This means pursuing efforts on miniaturizing systems to operate in situ and automating analytical methods to work on-line. The design, construction and evaluation of an automatic and low cost cyclic olefin copolymer (COC)-based spectrophotometric microanalyzer, capable of operating in unattended conditions, is presented to monitor soluble reactive phosphorous, as orthophosphate ion, in wastewater samples coming from sewage treatment plants. The microsystem, constructed by CNC micromilling and using a multilayer approach, integrates microfluidics to carry out the phosphomolybdenum blue (PMB) reaction and an optical flow-cell for the spectrophotometric orthophosphate determination in a single polymeric substrate smaller than a credit card. It is connected to a compact optical detection system composed by a LED emitting at 660 nm and a PIN-photodiode, both integrated in a PCB. Flow management is automatically performed by programmed microvalves and micropumps, which control autocalibration processes and allow unattended operation. Analytical features after the optimization of the microfluidic platform and the chemical and the hydrodynamic variables, were a linear range from 0.09 to 32 mg L−1 P and a detection limit of 0.03 mg L−1 P with a sampling rate of 24 samples h−1, demonstrating the microanalyzer suitability for SRP monitoring in water. Moreover, real samples were analyzed obtaining promising results.

Tunable cavity-enhanced terahertz frequency-domain optical Hall effect.

Presented here is the development and demonstration of a tunable cavity-enhanced terahertz (THz) frequency-domain optical Hall effect (OHE) technique. The cavity consists of at least one fixed and one tunable Fabry-Pérot resonator. The approach is suitable for the enhancement of the optical signatures produced by the OHE in semi-transparent conductive layer structures with plane parallel interfaces. Tuning one of the cavity parameters, such as the external cavity thickness, permits shifting of the frequencies of the constructive interference and provides substantial enhancement of the optical signatures produced by the OHE. A cavity-tuning optical stage and gas flow cell are used as examples of instruments that exploit tuning an external cavity to enhance polarization changes in a reflected THz beam. Permanent magnets are used to provide the necessary external magnetic field. Conveniently, the highly reflective surface of a permanent magnet can be used to create the tunable external cavity. The signal enhancement allows the extraction of the free charge carrier properties of thin films and can eliminate the need for expensive superconducting magnets. Furthermore, the thickness of the external cavity establishes an additional independent measurement condition, similar to, for example, the magnetic field strength, THz frequency, and angle of incidence. A high electron mobility transistor (HEMT) structure and epitaxial graphene are studied as examples. The tunable cavity-enhancement effect provides a maximum increase of more than one order of magnitude in the change of certain polarization components for both the HEMT structure and epitaxial graphene at particular frequencies and external cavity sizes.

Design and Fabrication of Optical Flow Cell for Multiplex Detection of β-lactamase in Microchannels.

Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip.

Real-time monitoring of nitrate in soils as a key for optimization of agricultural productivity and prevention of groundwater pollution

Abstract. Lack of real-time information on nutrient availability in cultivated soils inherently leads to excess application of fertilizers in agriculture. As a result, nitrate, which is a soluble, stable, and mobile component of fertilizers, leaches below the root zone through the unsaturated zone and eventually pollutes the groundwater and other related water resources. Rising nitrate concentration in aquifers is recognized as a worldwide environmental problem that contributes to water scarcity. The development of technologies for continuous in situ measurement of nitrate concentration in soils is essential for optimizing fertilizer application and preventing water resource pollution by nitrate. Here we present a conceptual approach for a monitoring system that enables in situ and continuous measurement of nitrate concentration in soil. The monitoring system is based on absorbance spectroscopy techniques for direct determination of nitrate concentration in soil porewater without pretreatment, such as filtration, dilution, or reagent supplementation. A new analytical procedure was developed to improve measurement accuracy while eliminating the typical measurement interference caused by soil dissolved organic carbon. The analytical procedure was tested at four field sites over 2 years and proved to be an effective tool for nitrate analysis when directly applied on untreated soil solution samples. A soil nitrate-monitoring apparatus, combining specially designed optical flow cells with soil porewater-sampling units, enabled, for the first time, real-time continuous measurement of nitrate concentration in soils. Real-time, high-resolution measurement of nitrate concentration in the soil has revealed the complex variations in soil nitrate concentrations in response to fertigation pattern. Such data are crucial for optimizing fertilizer application and reducing pollution potential of groundwater.

Automated analytical microsystem for the spectrophotometric monitoring of titratable acidity in white, rosé and red wines

The design, construction and evaluation of a low-cost cyclic olefin copolymer (COC)-based continuous flow microanalyzer with optical detection to determine the titratable acidity content of wine is here presented. The analysis method is based on the monitoring of the blue coloration decrease of a buffered bromothymol blue (BTB) solution in the presence of the acidic compounds of wine. The microanalyzer monolithically integrates the required microfluidic motifs as well as an optical flow cell where the measurements are performed by using a miniaturized and versatile photometric detection system.Fluid management is totally automated by the use of computer-controlled microvalves, permitting the automatic calibration of the system as well as the automatic sampling, including in-line dilution and analysis. The reduced size of the whole system along with its high simplicity and automation make it suitable for its application to the on-line monitoring of titratable acidity during wine-making processes.With the optimal conditions, a linear range up to 0.50 g L−1 tartaric acid, a quantification limit (LOQ) of 0.01 g L−1 and a detection limit (LOD) of 0.004 g L−1 were obtained, covering the most common acidity content of musts and wines. A sampling rate up to 26 h−1 could be achieved, consuming less than 3 mL of inexpensive reagents per analysis and requiring no pretreatment of the sample. The microsystem has been successfully applied to the quantification of the titratable acidity content of several wine samples, being the results in excellent agreement with the ones obtained by the reference method.

Experimental and simulation studies of the shape and motion of an air bubble contained in a highly viscous liquid flowing through an orifice constriction

This paper reports an experimental and computational study on the shape and motion of an air bubble, contained in a highly viscous Newtonian liquid, as it passes through a rectangular channel having a constriction orifice. The magnitude of the viscosity ratios, λ, and capillary numbers, Ca, explored is high: 5.5×105<λ<3.9×106 and 2.9<Ca<35.9 respectively. A multipass rheometer is used for the experimental work: air bubbles are suspended in 10 Pa s and 70 Pa s polybutene viscosity standards and passed through an orifice-plate geometry constructed within an optical flow-cell. High levels of bubble distortion are observed, including bubbles that resemble ‘crescent moons’. Simulation work is carried out using an implementation of the volume of fluid method in the freely-available finite-volume computational fluid dynamics code OpenFOAM. Quantitative data pertaining to the motion and shape of the bubble was extracted from both the experimental and simulation work. Initially, a good match between numerical simulation and experimental work could not be obtained: this problem was alleviated by changing the viscosity averaging method from an arithmetic mean to a logarithmically-weighted arithmetic mean. Medium- and high-resolution simulations using this new viscosity averaging method were able to match experimental data with coefficients of determination, R2, typically 0.898<R2<0.985.

Apparatus for Characterizing Gas-Phase Chemical Precursor Delivery for Thin Film Deposition Processes.

Thin film vapor deposition processes, e.g., chemical vapor deposition, are widely used in high-volume manufacturing of electronic and optoelectronic devices. Ensuring desired film properties and maximizing process yields require control of the chemical precursor flux to the deposition surface. However, achieving the desired control can be difficult due to numerous factors, including delivery system design, ampoule configuration, and precursor properties. This report describes an apparatus designed to investigate such factors. The apparatus simulates a single precursor delivery line, e.g., in a chemical vapor deposition tool, with flow control, pressure monitoring, and a precursor-containing ampoule. It also incorporates an optical flow cell downstream of the ampoule to permit optical measurements of precursor density in the gas stream. From such measurements, the precursor flow rate can be determined, and, for selected conditions, the precursor partial pressure in the headspace can be estimated. These capabilities permit this apparatus to be used for investigating a variety of factors that affect delivery processes. The methods of determining the pressure to (1) calculate the precursor flow rate and (2) estimate the headspace pressure are discussed, as are some of the errors associated with these methods. While this apparatus can be used under a variety of conditions and configurations relevant to deposition processes, the emphasis here is on low-volatility precursors that are delivered at total pressures less than about 13 kPa downstream of the ampoule. An important goal of this work is to provide data that could facilitate both deposition process optimization and ampoule design refinement.

Membrane Filtration of Anionic Surfactant Stabilized Emulsions: Effect of Ionic Strength on Fouling and Droplet Adhesion

Membranes hold great potential to be used for the successful treatment of oily waste water, but membrane fouling leads to substantial decreases in performance. Here we study the impact of ionic strength on membrane fouling from an emulsion stabilized by the anionic surfactant sodium dodecyl sulfonate (SDS). For this we use a unique combinatorial approach where droplet adhesion to a cellulose surface in a flow cell is compared to membrane fouling (flux decline) on a cellulose membrane. In the initial membrane fouling stages droplet adhesion dominates. While the flow cell demonstrates a high number of droplets adhering especially at high ionic strengths (100 mM NaCl), the strongest flux decline is observed at intermediate (10 mM NaCl) ionic strength. This suggests that the fouling mechanism must be different, with pore blocking expecting to dominate at intermediate ionic strength. At the later fouling stages the porosity of the cake layer plays a key role in the flux reduction. At low ionic strength, oil droplets repel each other strongly and an open, more permeable, cake layer is formed. However at higher ionic strength, a screening of charge interactions leads to a lower porosity and thereby a lower flux. This leads to a clear trend: with a higher ionic strength a higher flux decline is observed. Flux recovery is high at all ionic strengths, in line with the observation in the flow cell that oil droplets can easily be sheared of a cellulose surface at all ionic strengths. This work thus highlights the critical effect of the ionic strength on membrane fouling by anionically stabilized emulsions. Moreover it shows how the use of an optical flow cell can provide key insights to help explain observations in more standard membrane fouling experiments.

Micromachined optical flow cell for sensitive measurement of droplets in tubing

Here a micromachined flow cell with enhanced optical sensitivity is presented that allows high-throughput analysis of microdroplets. As a droplet flows through multiple concatenated measurement points, the rate of enzymatic reaction in the droplet can be fully characterized without stopping the flow. Since there is no cross-talk between the droplets, the flow cell is capable of continuously measuring biochemical assays in a droplet flow and thus is suitable to be used for continuous point-of-care diagnostics monitoring. This paper describes the design and operation of the device and its validation by application to the accurate and continuous quantification of glucose concentrations using an oxidase enzymatic assay. The flow cell forms an important component in the miniaturization of chemical and bio analyzers into portable or wearable devices.

ГАЗОАНАЛІЗАТОР ЯК ІНДИВІДУАЛЬНИЙ ЗАСІБ ЗАХИСТУ НАСЕЛЕННЯ З УДОСКОНАЛЕННЯМ

Purpose.The study or analysis of materials by determining their chemical or physical properties, namely, the analysis of materials using optical means, and can be used to control the ozone content in the atmosphe ric air, in the air of the working area, as well as in technological storage rooms for products under controlled conditions gas environment. Method. A device containing an ultraviolet radiation source, a measuring photodetector, an optical flow cell, a microprocessor meter, a reference photodetector installed with the ability to measure the intensity of ultraviolet radiation to the entrance to the optical flow cell, according to the invention,is additionally equipped with a radio receiver - transmitter. Results.The transmitter makes it possible to ensure the autonomous operation of the gas analyzer in the storage under the control of the microprocessor and transmit the measurement results to the central control panel without an operator visiting the storage. Thus, the stated differences can significantly improve the convenience and safety of the use of a gas analyzer compared with the prototype. Scientific novelty.Based on theoretical studies and research by improving the gas analyzer, in which modernization based on a new set and interrelation of structural elements, the convenience and safety of use is enhanced. Practical meaningfulness. Enhance the convenience and safety of use.

G-index: A new metric to describe dynamic refractive index effects in HPLC absorbance detection

High performance liquid chromatography (HPLC) with a solvent gradient and absorbance detection is one of the most widely used methods in analytical chemistry. The observed absorbance baseline is affected by the changes in the refractive index (RI) of the mobile phase. Near the limited of detection, this complicates peak quantitation. The general aspects of these RI-induced apparent absorbance effects are discussed. Two different detectors with fundamentally different optics and flow cell concepts, a variable-wavelength detector equipped with a conventional flow cell and a diode-array detector equipped with a liquid core waveguide flow cell, are compared with respect to their RI behavior. A simple method to separate static – partly unavoidable – RI effects from dynamic RI effects is presented. It is shown that the dynamic RI behavior of an absorbance detector can be well described using a single, relatively easy-to-determine metric called the G-index. The G-index is typically in the order of a few seconds and its sign depends on the optical flow cell concept.

Renewable chemiluminescence optosensors based on implementation of bead injection principle with multicommutation

In this work, the implementation of Bead Injection with multicommutation-based flow systems is reported. A surface renewable chemiluminescence (CL) flow sensor is presented based on the use of CL reaction of luminol with H2O2. Dowex 1 × 8 beads with immobilized luminol onto them were injected in the flow system by means of a six-port rotary valve and were accommodated into a 1 mm optical glass flow cell placed just in front of the rectangular photosensor window with the same size than the cell wall. Automatic computer-controlled manipulation of both reagents and sample solutions was undertaken using a multicommutated flow system which comprises five three-way solenoid valves, a home-made electronic interface and a Java-written software. Once the chemiluminescence signal was registered, sensing beads were automatically discarded out with a six-port rotary valve without needing to reverse or stop the flow. As a proof of concept and example, the enhancement of the chemiluminescence signal produced by Co(II) on the luminol-H2O2 reaction in alkaline medium was used for illustrating this implementation determining vitamin B12 in pharmaceutical preparations (after mineralization for releasing Co(II)). The analytical performance of the approach was satisfactory, showing a linear dynamic range from 1.7 to 50 µg L−1, a detection limit of 0.5 µg L−1, RSD (%) of 5.3%, with a sampling frequency of 11 h−1. The proposed approach was applied to different samples and the results were consistent with those obtained with a reference method based on ICP-MS. Based on the same reaction (or re-configuring the system to accommodate it to reaction requirements) the approach can also be applied to the determination of other metal ions such as Cr(III) and Fe(II) and appropriately extended to molecules of bioanalytical interest based e.g. in CL immunoassays, given its versatility.

Line-Focused Optical Excitation of Parallel Acoustic Focused Sample Streams for High Volumetric and Analytical Rate Flow Cytometry.

Flow cytometry provides highly sensitive multiparameter analysis of cells and particles but has been largely limited to the use of a single focused sample stream. This limits the analytical rate to ∼50K particles/s and the volumetric rate to ∼250 μL/min. Despite the analytical prowess of flow cytometry, there are applications where these rates are insufficient, such as rare cell analysis in high cellular backgrounds (e.g., circulating tumor cells and fetal cells in maternal blood), detection of cells/particles in large dilute samples (e.g., water quality, urine analysis), or high-throughput screening applications. Here we report a highly parallel acoustic flow cytometer that uses an acoustic standing wave to focus particles into 16 parallel analysis points across a 2.3 mm wide optical flow cell. A line-focused laser and wide-field collection optics are used to excite and collect the fluorescence emission of these parallel streams onto a high-speed camera for analysis. With this instrument format and fluorescent microsphere standards, we obtain analysis rates of 100K/s and flow rates of 10 mL/min, while maintaining optical performance comparable to that of a commercial flow cytometer. The results with our initial prototype instrument demonstrate that the integration of key parallelizable components, including the line-focused laser, particle focusing using multinode acoustic standing waves, and a spatially arrayed detector, can increase analytical and volumetric throughputs by orders of magnitude in a compact, simple, and cost-effective platform. Such instruments will be of great value to applications in need of high-throughput yet sensitive flow cytometry analysis.

Optical Flow Cell for Measuring Size, Velocity and Composition of Flowing Droplets

Here an optical flow cell with two light paths is reported that can accurately quantify the size and velocity of droplets flowing through a microchannel. The flow cell can measure the time taken for droplets to pass between and through two conjoined light paths, and thereby is capable of measuring the velocities (0.2–5.45 mm/s) and sizes of droplets (length > 0.8 mm). The composition of the droplet can also be accurately quantified via optical absorption measurements. The device has a small footprint and uses low-powered, low-cost components, which make it ideally suited for use in field-deployable and portable analytical devices.