Abstract
Miniaturized quantitative assays offer multiplexing capability in a microfluidic device for high-throughput applications such as antimicrobial resistance (AMR) studies. The detection of these multiple microchannels in a single microfluidic device becomes crucial for point-of-care (POC) testing and clinical diagnostics. This paper showcases an optical flow cell for detection of parallel microchannels in a microfluidic chip. The flow cell operates by measuring the light intensity from the microchannels based on Beer-Lambert law in a linearly moving chip. While this platform could be tailored for a wide variety of applications, here we show the design, fabrication and working principle of the device. β-lactamase, an indicator of bacterial resistance to β-lactam antibiotics, especially in milk, is shown as an example. The flow cell has a small footprint and uses low-powered, low-cost components, which makes it ideally suited for use in portable devices that require multiple sample detection in a single chip.
Highlights
Point-of-care (POC) and clinical diagnostics require state of the art quantitative assays at high throughput and in real-time near the patient
POC diagnostics devices are widely used in the market for certain tests such as urinalysis, pregnancy tests, blood glucose levels, sepsis, prostate and ovarian cancer and cardiovascular diseases
On the other hand, typically uses a light source to illuminate the sample in the microchannel and unlike the electrochemical sensor made of an electrode that is usually embedded inside the channel; the optical detector could move from one channel to another and scan many microchannels in sequence in a short period of time [10]
Summary
Point-of-care (POC) and clinical diagnostics require state of the art quantitative assays at high throughput and in real-time near the patient. The requirement to perform multiplex diagnostics in a single POC device has led to the development of multiple microchannel systems capable of loading multiple samples [6]. On the other hand, typically uses a light source to illuminate the sample in the microchannel and unlike the electrochemical sensor made of an electrode that is usually embedded inside the channel; the optical detector could move from one channel to another and scan many microchannels in sequence in a short period of time [10]. Most widely used optical detection methods include ultraviolet/visible (UV/Vis) absorption spectroscopy, fluorescence, chemiluminescence [7] and smartphone detections [11]. Detection of several microchannels requires multiple LED/Photodiode arrays with multiple electrical connections in series This is impractical due to the variations in sensitivities between detectors, increased costs and bigger size of the device. The microchannels pass through the detector one by one using a homemade linear actuator and a signal is recorded continuously in 15 microchannels
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