Number Of Subcultures Research Articles

Molecular Mechanism During Mycelium Subculture Degeneration of Volvariella volvacea.

Periodic mycelial subculture is a method commonly used for the storage of edible mushrooms, but excessive subculturing can lead to the degeneration of strains. In this study, the Volvariella volvacea strain V971(M0) was successively subcultured on PDA medium every 4 days, and one generation of strains was preserved every 4 months. Thus, five generations of subcultured strains (M1-M5) were obtained after 20 months of mycelial subculturing, their production traits were determined, and transcriptomic analysis was performed using RNA-seq; the differentially expressed genes were verified via RT-qPCR. The results showed that as the number of subcultures increased, the diameter of the mycelium and biological efficiency gradually decreased; in addition, the time in which the primordium formed increased and the production cycle was lengthened, while strains M4 and M5 lacked the ability to produce fruiting bodies. There were 245 differentially expressed genes between the M1-M5 and M0 strains, while the highest number of differentially expressed genes was between M3 and M0, at 1439; the smallest number of differentially expressed genes was between M2 and M0, at 959. GO enrichment analysis showed that the differentially expressed genes were mainly enriched in metabolic processes, organelle components, and catalytic activities. KEGG enrichment analysis showed that the differentially expressed genes were mainly enriched in metabolic pathways. The further annotation of differentially expressed genes showed that 39, 24, and 24 differentially expressed genes were related to substrate degradation, amino acid synthesis and metabolism, and reactive oxygen species metabolism, respectively. The downregulation of the related differentially expressed genes would lead to the excessive accumulation of reactive oxygen species, inhibit nutrient absorption and energy acquisition, and lead to the degradation of V. volvacea. These findings could form a theoretical basis for the degeneration mechanism of V. volvacea, and also provide a basis for the molecular function study of the genes related to strain degradation.

From Culture to Cultures: Understanding Edward Sapir’s Prophecy through Herderian Lenses

In 1934, the American anthropologist, Edward Sapir made a scientific prophecy on the concept of culture which remained largely overlooked but have proved to be surprisingly accurate and relevant in the contemporary era. He was focused on conceptual pluralisation and fragmentation. By predicting the pluralisation of culture, Sapir captured a trend that we can witness nowadays in the continuously growing number of cultures, subcultures, counterculture, and new conceptual interpretations and ramifications. His use of the Herderian concept of culture(s) requires a close inspection facilitated by interdisciplinary methodologies such as historical epistemology. Widely credited as the creator of the term “culture” in its plural sense, Johann Gottfried von Herder played a crucial role for Sapir’s academic formation. In the ruptures and continuities that culture as a concept has experienced, one can find valuable epistemological insights. Sapir did not come up with a daring prediction out of an uninformed position. Putting Sapir’s prophetic statement in the context of his academic and professional journey and in the context of the general research trends of the twentieth century reveals a strong Herderian heritage intensified by the psychologisation of anthropology that has ultimately led to an increasingly powerful and progressively sophisticated pluralisation of culture.

Somatic Mutation Accumulations in Micropropagated Cannabis Are Proportional to the Number of Subcultures.

Advancements in micropropagation techniques have made it easier to produce large numbers of cannabis clones, but these methods may also introduce genetic instability over successive generations. This instability often manifests as somaclonal variation, characterized by the progressive accumulation of genetic mutations or epigenetic alterations with each subculture. In this study, we examined how mutations accumulate in cannabis clones subjected to 6-11 subcultures. Using genotyping-by-sequencing, we identified 9405 polymorphic variants across 70 clones. The analysis revealed a correlation between the number of subcultures and the frequency of these mutations, revealing that genetic changes accumulate over successive subcultures despite clones sharing the same chronological age. Furthermore, we evaluated the functional impacts of accumulated mutations, with particular attention to implications on gene function and overall plant health. While rare, 14 high-impact variants were identified in genes that are important for plant development. Notably, six variants were also found in genes related to cannabinoid and terpene synthesis pathways, potentially affecting the plant's biochemical composition. These findings highlight the need for genetic assessments in micropropagation protocols, impacting plant breeding and conservation. Understanding genetic variations in clonally propagated plants optimizes practices for stability. Crucial for cannabis and horticultural plants, it emphasizes techniques to prevent genetic decay and ensure viability.

Main Composition and Visual Appearance of Milk Kefir Beverages Obtained from Four Consecutive 24- and 48-h Batch Subcultures

Nowadays, there has been a significant rise in the consumption of kefir, a functional beverage touted for its perceived health benefits. To offer a high-quality beverage to consumers, it is imperative to scrutinize and fine-tune the fermentation process. This study seeks to investigate the impact of fermentation time and the number of subcultures on the physicochemical, microbiological, and volatile composition, as well as the visual appearance, of kefir beverages obtained from four consecutive 24- or 48-h batch subcultures. All fermented beverages exhibited low lactose, ethanol and acids levels, with counts of viable probiotic lactic acid bacteria and yeast exceeding 106 colony forming units/mL. The four kefir beverages from the 48-h batch subcultures notably showed the lowest total concentrations of volatile compounds, likely due to overfermentation and over-acidification of the beverages. This caused the separation of the whey and curd, along with the formation of large gas bubbles, negatively affecting the visual appearance of the products. These findings emphasize the importance of fine-tuning the fermentation process to ensure the production of high-quality kefir beverages that align with consumer preferences. The four beverages from the 24-h batch subcultures exhibited high microbiological and physicochemical stability during storage at 4 °C for 28 days.

Towards the fourth decade of IOPRI’s oil palm clones: Upcoming new variety

Experience of IOPRI on oil palm tissue culture would be four decades in 2024. The first decade was theperiod of oil palm clone development and field testing. The second decade was a setback for oil palm research because of a high percentage of oil palm clone abnormalities in the field. Moving on from these experiences, tissueculture systems such as: including improving the protocol, controlling the duration of callus induction, delimitating the number of subcultures, and building of oil palm clones database, were managed to be improved. In this way, alow abnormality level is under control and each clone is traceable. It is also noted that the oil palm clone provides20-30% higher production than that derived from DxP crossing seed due to the more uniform growth of generatedplants. Besides the protocol, ortet selection is also key to improvement. Ortets are elite plants from selected progeny testing or re-clone of the progeny testing. Molecular analysis is applied as a control of genetic variation possibility, which may occur in a particular phase due to specific stimuli. However, until the present time, a clonevariety has not been released yet. Therefore, in the fourth decade, mass production is expected and oil palm clonevarieties will officially be released and commercialized

Effect of Explant Source on Phenotypic Changes of In Vitro Grown Cannabis Plantlets over Multiple Subcultures

Drug-type cannabis is often multiplied using micropropagation methods to produce genetically uniform and disease/insect-free crops. However, micropropagated plantlets often exhibit phenotypic variation, leading to culture decline over time. In cannabis, the source of these changes remains unknown, though several factors (e.g., explant's sources and prolonged in vitro culture) can result in such phenotypical variations. The study presented herein evaluates the effects of explant sources (i.e., nodal segments derived from the basal, near-basal, middle, and apical parts of the greenhouse-grown mother plant) over multiple subcultures (4 subcultures during 235 days) on multiplication parameters and leaf morphological traits of in vitro cannabis plantlets. While initial in vitro responses were similar among explants sourced from different regions of the plant, there were significant differences in performance over the course of multiple subcultures. Specifically, explant source and/or the number of subcultures significantly impacted plantlet height, number of nodes, and canopy surface area. The explants derived from the basal and near-basal parts of the plant resulted in the tallest shoots with the greatest number of nodes, while the explants derived from the middle and apical regions led to shorter shoots with fewer nodes. Moreover, the basal-derived explants produced cannabis plantlets with shorter but wider leaves which demonstrated the potential of such explants for in vitro rejuvenation practices with minimal culture decline. This study provides new evidence into the long-term impacts of explant source in cannabis micropropagation.

ОСОБЕННОСТИ ИНСТИТУЦИОНАЛИЗАЦИИ МОЛОДЕЖНЫХ СУБКУЛЬТУР НА РЕГИОНАЛЬНОМ УРОВНЕ В РАМКАХ ТЕОРИИ МАЛЫХ ГРУПП (НА ПРИМЕРЕ ВОРОНЕЖСКОЙ ОБЛАСТИ)

The purpose of the study is in the comprehensive and multidimensional study of the problem of integration of youth subcultures’ representatives into movements created at the initiative of federal and regional authorities, as well as volunteer and volunteer initiatives. The research methodology consisted in using the sociological method, which made it possible to evaluate the nominated candidates in terms of their sociological portraits. Youth subcultures are considered at the present stage of development of society, as well as the degree of involvement of young people in involvement in subcultures. Based on the analysis of the conducted studies, a conclusion is made about the possibilities of using the potential of youth subcultures and volunteer associations in the system of social management of society. Questions are concretized regarding the varieties of subcultures, the terminology of volunteering (volunteering). As a result, it is noted that at the moment representatives of youth subcultures are in no hurry to integrate into the official state-supported movement. In this regard, it is necessary to understand the motives and causes of such a paradox, as well as to use it for further work with these associations. As a result, the author draws conclusions about the attitude towards subcultures in the modern youth environment, as well as about the use of their potential in the activities of the authorities and administration of the region. On the one hand, interest in a number of subcultures is gradually fading away, on the other hand, young people are trying to find themselves, often not knowing what programs and actions exist aimed at people aged 18 to 35.

In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

Plant genetic resources conservation may be a potential option for the improvement of agricultural crops through modern biotechnologies, and in vitro conservation is a tool available to safeguard plant biodiversity. Ex situ conservation of plant genetic resources using the in vitro procedures is in progress in many countries. The slow growth storage (SGS) technique is a valid in vitro approach to preserve several vegetatively propagated species by controlling the growth and development of plantlets, economizing storage space and labor and reducing costs. Moreover, SGS prolongs the timing between subcultures, lowers the risk of losing germplasm through handling errors, such as contamination problems, and decreases the risk of genetic instability due to the reduction in the number of subcultures. SGS is applied by considering different factors: temperature, light or darkness conditions, medium composition, including mineral or sucrose concentrations, and the presence/absence of plant growth regulators, osmotic agents and growth inhibitors. SGS protocols for some fruit species have been well defined, others require additional research. The present review focuses on the effect of several factors that influence the SGS of in vitro shoots derived from temperate and tropical fruit species during the last ten years.

Machine Learning Deciphers Genotype and Ammonium as Key Factors for the Micropropagation of Bryophyllum sp. Medicinal Plants

Bryophyllum constitutes a subgenus of succulent plants that have been widely employed worldwide in traditional medicine. Micropropagation is required to optimize their growth and reproduction for biotechnological purposes. The mineral composition of culture media is usually an underestimated factor in the design of the in vitro culture protocols of medicinal plants. Universal and highly cited media mineral formulations, such as the Murashige and Skoog (MS) medium, are generally employed in plant tissue culture studies, although they cause physiological disorders due to their imbalanced mineral composition. In this work, neurofuzzy logic is proposed as a machine-learning-based tool to decipher the key factors (genotype, number of subcultures, and macronutrients) that are involved in the establishment of the Bryophyllum sp. in vitro culture. The results show that genotype played a key role, as it impacts both vegetative growth and asexual reproduction in all of the species that were studied. In addition, ammonium was identified as a significant factor, as concentrations above 15 mM promote a negative effect on vegetative growth and reproduction. These findings should be considered as the starting point for optimizing the establishment of the in vitro culture of Bryophyllum species, with large-scale applications as biofactories of health-promoting compounds, such as polyphenols and bufadienolides.

Establishment methods and research progress of livestock and poultry immortalized cell lines: A review.

An infinite cell line is one of the most favored experimental tools and plays an irreplaceable role in cell-based biological research. Primary cells from normal animal tissues undergo a limited number of divisions and subcultures in vitro before they enter senescence and die. On the contrary, an infinite cell line is a population of non-senescent cells that could proliferate indefinitely in vitro under the stimulation of external factors such as physicochemical stimulation, virus infection, or transfer of immortality genes. Cell immortalization is the basis for establishing an infinite cell line, and previous studies have found that methods to obtain immortalized cells mainly included physical and chemical stimulations, heterologous expression of viral oncogenes, increased telomerase activity, and spontaneous formation. However, some immortalized cells do not necessarily proliferate permanently even though they can extend their lifespan compared with primary cells. An infinite cell line not only avoids the complicated process of collecting primary cell, it also provides a convenient and reliable tool for studying scientific problems in biology. At present, how to establish a stable infinite cell line to maximize the proliferation of cells while maintaining the normal function of cells is a hot issue in the biological community. This review briefly introduces the methods of cell immortalization, discusses the related progress of establishing immortalized cell lines in livestock and poultry, and compares the characteristics of several methods, hoping to provide some ideas for generating new immortalized cell lines.

Genetic stability of virulent, intermediate, and avirulent strains of Ralstonia solanacearum after extensive, consecutive subculturing

An avirulent strain, FJAT1458, was demonstrated to possess a high level of biocontrol efficiency against bacterial wilt (BW). Assessing the genetic stability of this avirulent strain is essential for ensuring its safety and efficacy for use in field applications. In the present study, the genetic stability of three R. solanacearum strains with different levels of virulence was evaluated over the course of 50 generations of subculturing. Pathogenicity tests revealed that the virulent strain, FJAT91, and the intermediate strain, FJAT445, transformed into avirulent strains, but FJAT1458 remained avirulent after the same number of subcultures. Colonization tests revealed that the colonization pattern of the F1 generation of the avirulent FJAT1458 strain was similar to that of the F50 generation, but the virulent strain was rarely detected in later stages of infection. High performance liquid chromatography revealed that the number and type of chromatographic peaks produced by the avirulent strain did not exhibit any changes over the course of subculturing, however, the pattern of peaks produced by the virulent and intermediate strains changed noticeably over the course of 50 generations of subculturing. Moreover, exopolysaccharide I (EPS I) content, β-1,4-endoglucanase (Egl) activity, and swimming and swarming motility significantly decreased in the virulent and intermediate strains but were stable in the avirulent strain over the course of subculturing. The relative expression level of hrpB, phcA, eps, and egl, genes associated with virulence, significantly decreased in the virulent and intermediate strains over the course of 10 to 20 generations of subculturing but remained relatively stable in the avirulent strain across 50 generations. In conclusion, the avirulent strain remained genetically stable, while the virulent and intermediate strains exhibited genetic instability, over the course of 50 generations of subculturing.

Optimizing Turmeric Tissue Culture, Testing Different Media and a Plant Growth Regulator Matrix

Rapid multiplication of turmeric (Curcuma longa) by micropropagation is needed to produce a continuous source of uniformly sized, high-quality, and disease-free plantlets. Three in vitro experiments were conducted to optimize the medium by evaluating nine media and a full factorial combination (matrix) of two plant growth regulators for direct organogenesis of ‘Hawaiian Red’ turmeric. Two experiments evaluated the media, and the third studied the plant growth regulator matrix. As a result, Driver and Kuniyuki walnut (DKW), Murashige and Skoog (MS), and broadleaf tree basal (BLT) media performed better than woody plant media [Lloyd & McCown woody plant basal medium (L&M), and McCown’s woody plant basal salt mixture (McCown)] for shoot and root formation. The multiplication rate was 18 plants per explant in DKW with 1 mg⋅L−1 6-benzylaminopurine (BAP) and 0.1 mg⋅L−1 1-naphthaleneacetic acid (NAA). After transferring the plants to an ex vitro environment, the survival rate was 97%, and 30% higher than previously reported. DKW produced the highest number of plantlets (with shoots and roots), and BLT produced fewer plants with higher biomass. In the MS media, higher BAP to NAA ratio (2.5 to 0.1 mg⋅L−1) produced the most significant number of shoots; however, the lowest concentration of BAP and NAA (0.1 mg⋅L−1 of both) produced the highest number of rooted plantlets. There are two recommendations for tissue culture of ‘Hawaiian Red’ turmeric. To produce the highest number of plantlets, one should use the higher BAP to NAA ratio (2.5 mg⋅L−1 BAP and 0.1 mg⋅L−1 NAA) for shoot proliferation and then transfer the explants to the root initiation media. However, to reduce the number of subcultures, the explants can be grown in the lowest concentration of both BAP and NAA (0.1 mg⋅L−1) to induce both shoot and root. Although, the number of plantlets (with roots and shoots) will decrease in this method, there is no need for subsequent subcultures and changing of the plant growth regulator combinations.

Long-term subculture affects rooting competence via changes in the hormones and protein profiles in Cedrela fissilis Vell. (Meliaceae) shoots

Long-term subculture plays an essential role in the large-scale multiplication and production of somatic plantlets. We investigated the effects of long-term subculture on in vitro shoot development and ex vitro rooting associated with changes in the hormones and protein profiles in Cedrela fissilis. The number of subcultures of shoots induced a decrease in the ex vitro rooting response. The reduction in adventitious root (AR) formation was associated with decreases in the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), 12-oxo-phytodienoic acid (OPDA), putrescine (Put), and spermine and increases in jasmonic acid (JA), jasmonoyl-isoleucine, trans-cinnamic acid, and salicylic acid contents in shoots at the fourth subculture compared to the first. The ornithine decarboxylase enzyme preferentially functions in the Put biosynthesis pathway, and it was related to the highest AR formation in shoots at the first subculture. Down-accumulation of the auxin-binding protein ABP19a in shoots from the fourth subculture compared to the first subculture was related to a decrease in both the IAA content and AR formation. In addition, down-accumulation of glucose-6-phosphate isomerase; glutamine synthetase leaf isozyme, chloroplastic; 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase; l-ascorbate peroxidase, cytosolic; monodehydroascorbate reductase; and 2-Cys peroxiredoxin BAS1-like, chloroplastic and up-accumulation of caffeoyl-CoA O-methyltransferase 1 and isoforms of peroxidase 4 proteins in shoots from the fourth relative to the first subculture were associated with a reduction in AR formation. These results showed that the understanding of hormonal and molecular mechanisms related to the potential of AR formation in shoots under successive subcultures is relevant to improving large-scale plantlet production in C. fissilis.

Writings of the Luddites

When I mentioned to a friend that I was going to review this book, he replied: “Luddites? You mean, the Flat Earthers?” There is a distinction, I reminded him, between science and technology, and the Luddites had no problem, qua Luddites, with modern science. They objected to a few machines that were ruining their lives as artisans in the English textile industry. I could have added that Jeffrey Burton Russell's book Inventing the Flat Earth demonstrates that, “with extraordinarily few exceptions, no educated person in the history of Western Civilization from the third century BC onward believed that the Earth was flat” and that even the belief that people had believed the Earth was flat did not arise until around 1870, in a time of controversy between scientists and the general culture over the theory of evolution. My friend is not a historian or scientist (but then neither am I), and I concluded that his remark meant no more than “It's high time you got a cell phone.” When reading an article on historiography and genetics some time later, however, I came across a famous geneticist quoted as saying that, among anthropologists and historians of prehistory, “there are a small number of Luddites who want to break our machines.” He meant that the anthropologists and historians resented the interference of geneticists in their disciplines. But the Luddites held no brief against transmission electron microscopy or carbon dating or microbiome sequencers.Misunderstandings and misapplications of the term Luddites (which, literally, means followers of the English general Ned Ludd, who never existed but was said at the time to live, like Robin Hood, in Sherwood Forest) are regarded as artifacts of something called “Luddite silence.” The Luddites, it has been taught, were more or less illiterate and left no texts to interpret. The aim of Binfield's Writings of the Luddites—an annotated collection of poems, songs, manifestos, and threatening letters written between 1811 and 1817—is to demonstrate that “the Luddites were not silent.” Their opponents constructed the myth of silence by “ignoring, reducing, or rendering curious or impotent those Luddite texts . . . known to exist.” The Luddites’ chief opponents were not engineers, let alone scientists, but “dishonorable capitalist depredators” who were met with “the sturdy self-reliance of a community prepared to resist for itself the notion that market forces rather than moral values should shape the fate of labor.” The myth that “the poor stockinger, the Luddite cropper, the ‘obsolete’ hand-loom weaver,” and “the ‘utopian’ artisan” were silent resisters of industrial progress was a product, according to E. P. Thompson, of “the enormous condescension of history” to their social class. Writings of the Luddites may be understood as participating in Thompson's project of restoring to view, in Binfield's words, “the existence of a number of English subcultures with constitutive codes of their own.” Herbert Butterfield argued that English society of the Luddite era had become “dangerously diverse” and was only saved from revolution by the encouragement of belief, in all classes and subcultures, in historical progress (“the Whig interpretation of history”). Indeed, an anonymous Luddite letter of 1812 in this collection, addressed to “the principle Magistrate for this District” (Milnsbridge near Huddersfield), avers that “if this Machinery is suffer'd to go on it will probable terminate with a Civil War.”The Luddites lit many fires and destroyed much property in 1811–12, and as I write it is impossible not to think about the storming of the US Capitol on January 6 of this year. But the disaffected intruders of 2021, while contemptuous of science (only a handful wore masks, in a crowd, during a pandemic), clearly adored the technology that “capitalist depredators” had persuaded them they needed to buy. They were all carrying “smartphones,” taking “selfies”—and their leader was in close touch with them on Twitter.

Historical Evolution of Knowledge: Interpretation of Truth in Postmodernism

Changes in society lead to changes in science, changing its fundamental foundations - ideological and value, methodological and instrumental foundations. In turn, a change in the scientific paradigm leads to social change. A situation of this kind is especially evident in the socio-political sciences, since it is they who are more affected by social reality. The nature of postmodern societies is such that its economic component generates a huge number of subcultures, in the scientific world it is called "subcultural explosion." Postmodern societies are societies of permanent changes: consumer standards, moral values, political structure, and so on, repeatedly change during the life of one generation, significantly affect the search for the truth of science and its understanding. In the list of various questions of truth, its character, origin, postmodern departs from objective truth, therefore it becomes contextual and consensus. Theories are replaced by approaches, views, points of view, thoughts. Scientific concepts with their strict certainty change concepts, in which under the same name everyone puts their own meaning and essence. According to this, in postmodernism, not only the scientific paradigm as a model of scientific activity is changing, but the purpose of science, its understanding as a special activity with the search for truth, is changing. This implies a review of the attitude to the "organizational forms" of science, that is, the way in which scientific knowledge is disseminated to the professional scientific community to search for truth, which in its classical understanding no longer exists.

Gene expression profiling of primary fibrochondrocyte cultures in traumatic and degenerative meniscus lesions.

This study aimed to investigate how fibroblastic and chondrocytic properties of human meniscal fibrochondrocytes are affected in culture conditions according to the type of meniscal pathology and localization, and to provide basic information for tissue-engineering studies. Primary fibrochondrocyte cultures were prepared from meniscus samples of patients who had either traumatic tear or degeneration due to osteoarthritis. Cultures were compared in terms of mRNA expression levels of COL1A1, COL2A1, COMP1, HIF1A, HIF2A, and SOX9 and secreted total collagen and sulfated sGAG levels according to the type of meniscal pathology, anatomical localization, and the number of subcultures. mRNA expression levels of COL1A1, COMP1, HIF1A, HIF2A, and SOX9 were found to be increased in subsequent subcultures in all specimens. COL1A1 mRNA expression levels of both lateral and medial menisci of patients with traumatic tear were significantly higher than in patients with degenerative pathology, indicating a more fibroblastic character. P1 subculture of lateral and P3 or further subculture of medial meniscus showed more fibroblastic characteristics in patients with degenerative pathology. Furthermore, in patients with degenerative pathology, the subcultures of the lateral meniscus (especially on the inner part) presented more chondrocytic characteristics than did those of medial meniscus. The mRNA expression levels of the cultures showed significant differences according to the anatomical localization and pathology of the meniscus, indicating distinct chondrocytic and fibroblastic features. This fundamental knowledge would help researchers to choose more efficient cell sources for cell-seeding of a meniscus scaffold, and to generate a construct resembling the original meniscus tissue.

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro regeneration of C. officinalis has been successfully established but somaclonal variation was not evaluated. The regeneration pathway and the number of subcultures on somaclonal variation were evaluated using six ISSR primers that amplified 58 loci of Inter Simple Sequence Repeats (ISSR). A dendrogram based on Jaccard´s genetic distance between the subcultures and the donor plant was produced. The results show that indirect shoot regeneration induces somaclonal variation, in the presence 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with kinetin and 6-Benzylaminopurine (BAP). In combination with 1-Naphthaleneacetic acid (NAA) or with Indole-3-butyric acid (IBA), BAP produces genetically stable explants. The highest proliferation rate was achieved using BAP and IBA. The present research study suggests avoiding the use of 2,4-D when C. officinalis is propagated for reintroduction and restoration projects.

Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don.

This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, antioxidant capacity, and enzyme inhibitory potential of in vitro-established callus, somaclonal variant, and normal green shoots of Catharanthus roseus. The highest shoot induction rate (95.8%) and highest number of shoots (23.6), with a mean length of 4.5 cm, were attained when the C. roseus nodal explants (0.6–1 cm in length) were cultivated in Murashige and Skoog (MS) medium with 2 µM thidiazuron, 1 µM 2-(1-naphthyl) acetic acid (NAA), and 4% sucrose. The in vitro flowering of C. roseus was affected by sucrose, and the number of subcultures had a significant effect on shoot multiplication and somaclonal variation. The highest levels of phenolics and flavonoids were found in normal green shoots, followed by those in somaclonal variant shoots and callus. The phytochemicals in C. roseus extracts were qualified using liquid chromatography–tandem mass spectrometry. A total of 39, 55, and 59 compounds were identified in the callus, somaclonal variant shoot, and normal green shoot tissues, respectively. The normal green shoot extracts exhibited the best free radical scavenging ability and reducing power activity. The strongest acetylcholinesterase inhibitory effects were found in the callus, with an IC50 of 0.65 mg/mL.

Comparative study on in vitro micropropagation response of seven globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars: open-pollinated cultivars vs F1 hybrids

Globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] growing has gained commercial importance in recent years due to its consumption as food. It has also started to attract attention in pharmaceutics. Due to globe artichoke’s stated importance, growers need large amount of pathogen-free, healthy starting materials for production. Stated material will maximize the yield while minimizing the costs. Hybrid cultivars have uniform in height and maturity and could be harvested concurrently; on the other hand, an open-pollinated cultivar would have useful potential that could be smoothly produced locally at a lower cost. In vitro micropropagation enabling these goals as it serves large scale, fast, reliable and realistic alternative method to classic propagation via offshoots. The aim of the present study was to comparatively evaluate the micropropagation efficiency of two important local open-pollinated (OP) cultivars (‘Bayrampaşa’, ‘Sakız’) and five F1 hybrid cultivars (‘Olympus’, ‘Madrigal’, ‘Sambo’, ‘Green Globe’, ‘Imparator’), on the basis of total subcultures they were subjected to. Various plant growth regulators at various combinations were assessed for in vitro micropropagation and subsequent in vitro rooting. 3/4 basic MS medium supplemented with 0.05 mg L-1 BA + 0.005 mg L-1 IBA was determined as the best media combination for in vitro micropropagation, while 10.0 mg L-1 IAA + 1.0 g L-1 activated charcoal adding to 1/2 basic MS medium had positive effects on in vitro rooting. According to results, the micropropagation efficiency varied based on cultivar differences and number of subcultures regardless of being OP or F1 hybrid. The present study demonstrated that in vitro propagation of globe artichoke could be a valuable process for assessing mass propagation regardless of using F1 or OP cultivars. Considering the OP cultivars are cheap in terms of price in a comparison to F1 hybrid cultivars, OP cultivars could be also recommended to be used for in vitro mass propagation.

Somatic embryogenesis and plant regeneration in Ferula jaeschkeana Vatke: a threatened medicinal herb

An efficient in vitro propagation protocol for an important medicinal endangered herb Ferula jaeschkeana has been developed through somatic embryogenesis. 12 week old friable calli were derived from petiole explants on a medium supplemented with 2.0 mgl−1 2,4-D and 1.0 mgl−1 Kn and used as mother source for somatic embryogenesis. Different auxins (2,4-D, IBA, IAA and NAA) in different concentrations (0.2–4.0 mgl−1) was used to produce embryogenic callus. Eighty four percent calli produced globular embryos on a medium supplemented with 4.0 mgl−1 2,4-D. The globular embryo then mature on culture medium (Control) or supplemented with different addictives (auxin, proline and ABA), maturation was significantly affected by ABA, proline and number of subcultures with maximum number of mature embryo (32.1 ± 0.2) was formed on medium fortified with 2.0 mgl−1 proline. Mature embryos had two well-developed cotyledons and an elongated hypocotyl root axis. 78% of these embryos were transformed into plantlets when placed in culture medium fortified with 0.2 + 0.01 + 0.05 mgl−1 (BAP:IBA:GA3) respectively. Regenerated plants were hardened in vermiculite with a 23% survival rate. The regenerated plants showed normal diploid chromosome number (2n = 22) and leaf morphology was comparable to in vivo plant.