Number Of Micromycetes Research Articles

Glyphosate Effects on Some Characteristics of Biological Activity and Phytotoxicity of Sod-Podzolic Soil in a Short-Term Model Experiment

In a model laboratory experiment on infertile arable soil with low biological activity, it was found that the introduction of glyphosate leads to a short-term change in the intensity of the main processes of microbial transformation of nitrogen in the soil. When incubating soil with glyphosate at the maximum recommended dose of 8 l/ha for 22 days, there is an increase in nitrogen-fixing and denitrifying activity by 30–80% and 300% and a decrease in the nitrification process by 20–40%. The effects are of a short-term nature and do not reflect the entire complex of ongoing microbiological processes: no effect of glyphosate was detected on the emission of CO2, which is an integral indicator of biological activity. At the end of incubation in the soil with the introduced glyphosate, there was an increase in the number of bacteria by 40% and a decrease in the number of micromycetes by 70%. In general, under the selected conditions, the introduction of glyphosate led to a marked deterioration in the biological activity of the soil. By the method of multisubstrate testing, it was shown that under the action of the herbicide there is an increase in the value of the coefficient of rank diversity of the consumption spectra of substrates d, accompanied by a decrease in the specific metabolic work W and the integral vitality index G. It was shown for the first time that when glyphosate is introduced into soil with low biological activity and availability of phosphorus and the herbicide is degraded along the sarcosine pathway with a break in the C–P bond, excluding the formation of toxic metabolites, there is a pronounced negative effect of glyphosate on soil microorganisms, which leads to inhibition of wheat plant growth.

Impact of radioactive contamination of soils on the diversity of micropopulation and the transformation of organic substances

Aim. To study the impact of different levels of radioactive contamination on the organic matter decomposition and the population development of microbial decomposers of organic matter in soil. Methods. Gamma-spectrometry for the determination of the relative activity of 137Cs and beta-spectrometry for the determination of the relative activity of 90Sr in order to choose the contamination range for the studies; Tea Bag Іndex (TBI), the standard glob- ally accepted method to determine the rate of organic matter decomposition; gas chromatography – to determine the impact of the investigated factors on the formation of the biomass of microorganisms by means of the СО2 production potential; classic microbiological methods, using elective media to estimate the population densities of culturable microbial decomposers. Results. The studies (from April to September 2021) comprised two soils with different radioactive contamination ranges: Range No. 1 in Narodychi district of Zhytomyr region (the village Khrystynivka, 3 sampling points) in the unconditional (obligatory) resettlement zone after the catastrophe in the Chornobyl nuclear power plant (ChNPP) (it has an absorbed dose rate gradient, evaluated for soil microorganisms, from 0.2 μGy/h (at sampling point Narodychi-1: 51,24076°N, 29,21497°E) to 1.57 μGy/h (at sampling point Naro- dychi-3: 51,23815°N, 29,22245°E)), located on the agricultural field, abandoned after the catastrophe and Range No. 2, located on the afforested area, directly bordering the territory of the so-called Red Forest in the ChNPP ex- clusion zone (4 sampling points – the first three points – natural ecosystems, and 4th – pyrogenically transformed territory after fires in 2020) with the absorbed dose rate gradient from 3.7 μGy/h (at sampling point ChEZ-1: 51,38595°N, 30,03035°E) to 84.0 μGy/h (at sampling point ChEZ-4: 51,38231°N, 30,03298°E). The dependence of the studied indicators on the gradient of soil contamination with radionuclides was demonstrated and these are the conditions that the difference is more than 400 times in the values of the ionizing radiation absorbed dose rates formed by radionuclides in soil. In Range No. 1 (on the fallow land), with the increase in the dose rate up from 0.2 to 1.57 μGy/h, there was statistically significant increase of mineralization coefficient and percentage of reduced weight of rooibos tea between the least and most contaminated sampling points), stimulation of the development of micromycetes (by 1.2–2.3 times), cellulose-decomposing bacteria (by 7.1–7.9 times), and ammonifying bacteria (by 1.8–6.3 times) due to a significant increasing the number of colony-forming units in points with higher radio- nuclide contamination and an increase of microbial biomass in soil more than twofold between the least and most contaminated sampling point of Range No. 1.In Range No. 2, in the ChNPP exclusion zone, characterized by low parameters of sod-podzolic soil fertility (humus content from 1.2 ± 0.2 to 0.9 ± 0.1 %, exchangeable acidity from 4.0 ± 0.1 to 5.0 ± 0.7), the number of micromycetes and ammonifying bacteria in soil was one-two orders below the indices of Range No. 1. Micromycetes dominated in the groups of cellulose-decomposing microorganisms. There was statistically significant increase of mineralization coefficient and percentage of reduced weight of rooibos tea with increasing of radionuclide contamination level for the first three plots: ChEZ-1, ChEZ-2 and ChEZ-3 of Range No. 2. Conclusions. In the fallow land of the unconditional (obligatory) resettlement zone, the increase of the radia- tion absorbed dose rate in soil by one order from 0.2 to 1.57 μGy/h does not inhibit the development of microorgan- isms in soil, rather it stimulates their activity and increases their numbers. On poor sod-podzolic soils in the exclu- sion zone of the Chernobyl nuclear power plant, the preponderance of development of micromycetes over bacterial cellulolytics is noticeable. The radioactive contamination affected the development of soil microorganisms and the biological processes in soils not only in the first years after the Chornobyl catastrophe, which has been described in the literature, but has had its influence for rather a long time (for over thirty years after the accident). Among the microorganisms of a saccharolytic mode of organic plant residuals decomposition, the micromycetes dominate.

Влияние депонированной формы комплексного пестицидного препарата на структуру урожая зерновых культур и заболеваемость корневыми гнилями

The use of chemical plant protection products is essential to obtain a quality crop, but entails the risks of spreading pesticides in the biosphere and causing damage to beneficial biota. Therefore, the development of new approaches to the delivery of pesticides, which reduce the negative impact on agroecosystems, is an important direction for sustainable agriculture development. The effect of a complex preparation containing the fungicide tebuconazole and the herbicide tribunuron-methyl, embedded in a biodegradable polymer base of poly(3-hydroxybutyrate) and birch sawdust, on the several indicators was studied: the number of rhizospheric micromycetes of spring wheat cv. Novosibirskaya 15 and spring barley cv. Biom, the effectiveness of root pathogens control, and the structure and quality of the crop. In field experiments, a prolonged fungicidal action of the complex embedded preparation was demonstrated, which was superior in duration and efficiency to the traditional pre-sowing seed dressing. During the growing season, there was a significant (p <0.05) decrease in the number of micromycetes in the rhizosphere of wheat and barley by 1.5-2 times in comparison with the control, as well as a decrease in the prevalence of root phytopathogenic fungi (Alternaria, Bipolaris, and Fusarium). A high level of biological efficiency of the embedded fungicide was demonstrated in different phases of plant development - from 73.4 to 100% on wheat and from 68 to 100% on barley. As a result, the use of a complex embedded preparation increased the yield of spring wheat and barley, respectively, by 10.8 and 4.4 c/ha compared to the control and contributed to the improvement of the grain quality of crops. Keywords: PESTICIDES, EMBEDDING, TEBUCONAZOL, TRIBUNURON-METHYL, POLY(3-HYDROXYBUTYRATE), ROOT ROTS, YIELD, WHEAT, BARLEY

PHYTOPATHOGENIC MYCOBIOTA IN AGROCENOSES OF CULTURAL PLANTS IN THE CONDITIONS OF THE CENTRAL FOREST STEPPE OF UKRAINE

Intensive development of phytopathogenic micromycetes in the agrocenoses of cultivated plants leads to significant crop losses, deterioration of its quality and reduction of plant productivity. Phytopathogens are a powerful factor in biological pollution of agroecosystems. The purpose of our study is to determine the number and species composition of micromycetes on the leaves of sunflower plants of Dushko and Oliver hybrids and winter wheat of Podolyanka and Skagen varieties in the agrocenoses of the Central Forest Steppe of Ukraine. Studies of the mycobiome of the leaves of sunflower plants have shown that the number of micromycetes is significantly influenced by the biological features of the investigated sunflower hybrids and their cultivation technologies. It was established that the number of phytopathogenic micromycetes on the vegetative organs of the investigated hybrids under the organic technology of sunflower cultivation was at the level of the traditional technology and varied between 14.6 and 19.9 thousand CFU/g of dry leaf. The species composition of micromycetes on the leaves of sunflower plants was determined and it was established that the dominant fungi are the following genera: Aspergillus P. Micheli ex Haller, Alternaria Nees, Penicillium Link; Fr, Fusarium Link and Cladosporium Link. They were characterized by a different frequency of occurrence during the growing season, which ranged from 15 to 70%. According to the results of the research of the mycobiome of the leaves of winter wheat plants, it was established that the number of micromycetes on plant leaves ranged from 0.9 to 3.8 thousand CFU/g of dry leaf and significantly depended on the cultivation technology and biological characteristics of plants of different breeding origins. Metabolites of Podolyanka winter wheat plants under conditions of both traditional and organic growing technology stimulated the development of micromycetes in the mycobiome of vegetative organs of plants. Metabolites of Skagen winter wheat plants under conditions of different cultivation technologies restrained the development of micromycetes at an ecologically safe level. The species composition of micromycetes on the vegetative organs of winter wheat plants was determined, and it was established that under the conditions of traditional growing technology, the dominant micromycetes were the species F. oxysporum, F. graminearum, and B. sorokiniana with a frequency of occurrence of 55 to 70%. And in the conditions of organic cultivation technology - F. oxysporum and T.viride, the frequency of which was 50%. Micromycetes of these genera are toxin-producing species that contaminate agricultural crops with mycotoxins, which poses a danger to animal and human health.

Consequences of the Long-Term Fertilization System Use on Physical and Microbiological Soil Status in the Western Polissia of Ukraine

The response of soil microbial diversity to long-term fertilization is still not well understood in the context of different soil types. The purpose of this research was to reveal the impact of fertilization systems on soil parameters and life activity of the main taxonomic and physiological groups of microorganisms responsible for nitrogen, carbon, and phosphorus transformation. Reported results were obtained in the course of a 55-year-long experiment on fertilization of sod-podzolic soil in a grain-flax-potato crop rotation. Soil sampling was conducted within a 0–20 cm depth in five sites: without fertilizer (C); organic fertilization system, manure (O1FS); mineral fertilization system, NPK (MFS); organic-mineral fertilization system, manure + NPK (O1MFS); and organic-mineral fertilization system, siderate + NPK (O2MFS). Long-term use of various fertilization systems has led to changes in the soil properties. Bacteria dominated the microbial community in all examined areas. Soil fertilization supported bacteria development in all variants, except for MFS, and negatively affected the micromycetes content. A strong relationship between the change of the main soil indicators and the number of microorganisms from the main taxonomic groups was found between the soil pH KCl and the number of micromycetes. The O1FS option had the most beneficial effect on the development of soil nitrifiers and denitrifiers. The O1MFS fertilization system was the most favorable for the development of non-symbiotic anaerobic nitrogen-fixing, cellulose-degrading and phosphate-mobilizing microorganisms. In turn, the least favorable conditions for the development of physiological groups of microorganisms were found in cases of continuous use of mineral fertilizers.

Innovative technologies for the processing of raw materials of plant origin to improve the quality of food supply of the troops

The object of research: morphological and cultural characteristics of micromycetes: yeast Saccharomyces cerevisiae and mycelial fungi Mucorracemosus grown on agar medium.The subject of research: flax seed suspension, ultrafine powder of double oxide of divalent and trivalent ferrum, micromycetes: yeast Saccharomyces cerevisiae and mycelial fungi MucorracemosusInvestigated problem: ensuring the bacteriostatic properties of raw materials and obtaining products with an extended dates of expiry.The subject in scientific results: the impact of nanoparticles (NP FeO×Fe2O3) on the bacteriostatic (protective) properties of flax seeds against fungal infections has been studied and the dependence of bacteriostatic properties on the amount of nanomagnetite has been determined. The ability of nanomagnetite to improve the bacteriostatic properties of flax seeds has been proven: addition of 0.1 %; 0.15 %; 0.2 % of nanomagnetite suppresses the development of microflora (micromycetes) in flax seed samples by 8–20 times. A reduction in the number of micromycetes (yeast Saccharomycescerevisiae and mycelial fungi Mucorracemosus) by 8–10 times and the size by 10–20 times was established compared to the control.
 The rational content of nanoparticles (NPs FeO×Fe2O3–nanomagnetite) has been determined as 0.15 % of the weight of the recipe mixture.
 The proposed mathematical model makes it possible to predict the effectiveness of using NP FeO×Fe2O3 – nanomagnetite to inhibit the growth of mycelial fungi to ensure the bacteriostatic properties of raw materials, in particular, flax seeds.
 The area of practical use of the research results: food industry enterprises specializing in the production of bakery and flour confectionery products using a mixture of wheat and rye flour with the addition of food additives. Innovative technological product: flour raw material with a mineral nano-additive suspension based on the double oxide of divalent and trivalent ferrum, which allows to ensure quality and extend dates of expiry of the product. Scope of the innovative technological product: to enhance the quality of food supply of the troops in extreme conditions

Hygienic assessment of priority factors of labor conditions in modern medical organizations

Currently, one of the main tasks of the state and society is to preserve and strengthen the health of the able-bodied population. Employees of medical organizations are exposed to the combined effects of a significant number of harmful occupational factors. In particular, factors of the production environment include biological, chemical and physical, factors of the labor process — tension and severity. Therefore, the study of working conditions of medical personnel is a priority in occupational hygiene. The purpose of the study is to identify the priority production-related and occupational pathology in a medical organization. The object of the study was 1443 maps of special assessment of working conditions, the results of laboratory and instrumental studies of microclimate parameters, lighting (5398 measurements in 60 rooms), air samples for the content of microorganisms (727 samples). According to studies of the conditions and nature of work at the workplaces of medical workers in the studied medical organizations, according to the results of a special assessment of working conditions and their own research, they corresponded to the harmful class 1–3 and for cancer hospital workers of the 4th degree. The level of maximum microbial load in the resident and nursing rooms increased by the middle of the work shift and remained high until the end of the working day. Since the air velocity in the studied rooms was at a very low level, this criterion may be a risk factor for aggravating the course of general somatic pathologies and, as a consequence, the development of production-related morbidity among medical workers. It can also be noted that in 100 % of cases, fungi were detected, with this number decreasing by the end of the working shift, representatives of the genus Staphylococcus, on the contrary, tended to increase from the beginning of the worker to its end. The conditions and nature of the work of medical workers in the studied medical organizations, according to the results of a special assessment of working conditions, corresponded to a harmful class of 1–4 degrees. The 4th degree of the 3rd class of working conditions was due to the work of staff with cytostatic drugs. The microclimatic parameters corresponded to hygienic standards (at low air speeds). In 100 % of cases, fungi and representatives of the genus Staphylococcus were found in all air samples. A detailed study of the species identification of micromycetes in the indoor air showed that the number of micromycetes of the genus Penicillium and Aspergillus significantly exceeded the number of other species.

MYCOSES OF MUSTELIDS OF CELL CONTENT

The focus of attention of authoritative international organizations, such as the World Health Organization, the Food and Agriculture United Nations, is the problem of contamination by fungal microflora of feed and the formation of secondary metabolites (mycotoxins) and a number of micromycetes. Contaminant fungi are mainly represented by saprophytic species with disease-causing potential. Entering the body of an animal, fungi can cause invasive lesions of the gastrointestinal tract (mucoromycosis), infection by inhalation of fungal spores, followed by the development of respiratory mycoses (aspergillosis). Almost all types of molds are capable of synthesizing mycotoxins. Some types of fungi (Penicillium) form antibiotics as secondary metabolites, which, when ingested, can disrupt the composition of the microflora of the gastrointestinal tract. The article presents the results of studies on the identification of mycoses in mustelids of cell content. To determine the species composition of pathogenic fungi, most often found in mustelids in fur farms of the Russian Federation, microbiological studies were carried out using selective culture media and a MicroTax microbiological analyzer. As a result of laboratory research, it was found that among the isolated material from the internal organs, pathogenic fungi of the genera Fusarium, Aspergillus, Mucor, Candida are most often found. Microbiological examination of animal feed (porridge, grain grinding, fish feed) revealed identical pathogens. As a result of setting a bioassay on rabbits and white mice, the pathogenicity of the isolated fungi was determined. A laboratory study was carried out to study the effect of the isolated mushrooms and their metabolic products on the weasel organism.

QUANTITATIVE COMPOSITION THE MICROORGANISMS OF RHIZOSPHERE AGRICULTURAL PLANTS

During 2020, on the basis of stationary and temporary field experiments, which are located in the Skvуra Research Station of Organic Production of NAAS, Nosiv SDS and the Institute of Vegetable and Melon NAAS were studied quantitative composition of the soil micromycetes under different agricultural crops: winter wheat, spring barley and onion. The vegetation period in the Kyiv region was characterized by – sufficiently moist (HTC 1,7), and in Chernihiv and Kharkiv regions drought prevailed (HTC 0,6). Adverse weather conditions such as drought or waterlogging are crucial factors in changing the number of mycobiomes in the studied soils.Stationary field experiments in the selection Nosivka during the ontogenesis winter wheat characterized the greatest number pedatrophic and less number of amylolytic, cellulosolytic ecological-trophic groups. The stationary experiments field researches were also conducted in Skvyra Research Station of Organic Production. Where the rhizosphere soil under spring barley crops is characterized by a larger number of micromycetes, as well as amylolytic and cellulolytic ecological-trophic groups. Was determined the number of soil micromycetes the main ecological and trophic groups. The rhizosphere soil on the stationary field of the Institute of Vegetable and Melon NAAS during the ontogenesis of different varieties of onions characterized by high number cellulosolytic group of micromycetes, which despite the decrease in the hydrothermal coefficient increased by the end of the growing season of the culture.Therefore, the interaction between plants and micromycetes is a dynamic process in which the hydrothermal coefficient during the growing season, soil type, and the root system of agricultural plants, this influences the formation of soil mycobiome.

Assessment of the impact wastewater of dairy farms on soil microbiota

In the production of milk on cattle-breeding farms and complexes large amount of wastewater is formed containing significant numbers of chemicals and is ecologically hazardous source of pollution of water and soil resources. Wastewater of dairy farms contain natural animal excretions, exhaust solutions of cleaning and disinfecting agents, milk residue which is washed off from internal surfaces of milking equipment, residues of feed, litter, dirt, which is washed off during washing of walls and floor of dairy cow house. Since such wastewater contain valuable organic substances they are used to fertilize plants. There are technologies of safe use of wastewater from cattle-breeding farms to fertilize pastures. Also wastewater used as fertilizer in cultivation of sugar beets, tomatoes, cucumbers, spinach, cabbage and the like. It is expedient determination of the influence of wastewater used for the fertilizer of agricultural cultures, on the soil microbiota. After all, soil fertility is inextricably linked with microorganisms that inhabit her. The aim of the work was carrying out of an assessment of influence of wastewater from dairy farms on the soil microbiota. Samples of soil for microbiological studies were selected in 20 centimetre thickness of top layer after application of wastewater of dairy farms. The total microorganism number was determined the method of sowing of soil suspension of corresponding dilutions on nutrient medium of meat-and-peptone agar (MPA). Micromycetes on Chapek medium. The state of soil microorganisms after application in her different doses of wastewater of dairy farms was investigated. It is established, that the total number of microorganisms in soil when accounting 12 days later after application of wastewater in quantities of 500 l/ha exceeded control by 7.4%. At doses of 600 l/ha and 700 l/ha by 9.8% and 12.4% accordingly. 30 days later after application of waste-water the number of microorganisms almost did not differ from control. It was observed that after application of wastewater increase the total number of micromycetes. 12 days later after application of dose 500 l/ha, of number of micromycetes was increased on average by 14,6%; in dose of 600 l/ha by 18.0%; in dose of 700 l/ha — by 19.6%. 30 days later after application of wastewater the total number of micromycetes exceeded control by 1.5%. It is defined, that wastewater of dairy farms in doses of 500–700 l/ha do not exert negative effect on soil microbiota.

Influence of microbial preparations on biomorphological indicators of tulip (<i>Tulipa</i> L.) ‘Anna Krasavitsa’ and soil fertility under steppe Crimea conditions

The data on the study of the effect of treatment of tulip bulbs of the Anna Krasavitsa cultivar before planting with microbial preparations (MP) Aurill and the Complex of microbial preparations (CMP) on the growth, state, ability for vegetative propagation of tulips, as well as the fertility and biological activity of the tulip rhizosphere in Crimea have been presented in the article. The studies were carried out in 2017-2019 in a small-plot field experiment on the tulip collection site of the NBG-NSC (v. Novy Sad, Simferopol district of the Republic of the Crimea) on the southern carbonate chernozem. Tulip bulbs were treated with MP working suspensions before planting with a dose of the preparation equal to 2 % of the bulb mass. The control - treatment with tap water. The study, collection and processing of the obtained data on the state of plants, fertility and biological activity of the soil was carried out by generally accepted methods. As a result of the studies was found that MPs increased the coefficient of vegetative propagation of bulbs, the mass of the nest and replacement bulb by 6-23 %, at the same time, a positive effect of CMP on an increase in the mass of large bulbs was revealed in the axils of the storage scales. The features of the influence of the treatment of tulip bulbs ‘Anna Krasavitsa’ with Aurill and CMP preparations on the morphometric characteristics of the plant have been revealed: the height and size of the flower and the lower leaf were increased. A positive effect of the preparations on soil fertility under tulips was shown: CMP promoted an increase in the organic matter content in the soil by 0.19 %, Aurill - the accumulation of nitrate nitrogen and exchangeable potassium in the rhizosphere of a tulip, and increasing its biological activity. The use of MP increased the number of ammonifying and amylolytic bacterial groups in the rhizosphere of the tulip; to a greater extent, Aurill, CMP increased the number of phosphate-mobilizing (by 58 %), as well as oligotrophic and cellulolytic bacteria by 8 and 78 % respectively. Aurill suppressed the number of micromycetes by 48 %.

Influence of Philazonit biopreparation on the mycobiome of soybean plants rhizosphere

The article presents the results of analysis of biodiversity and spatial-functional structure of the microbial complex in the soil rhizosphere, and development of ways to regulate the number of phytopathogenic micromycetes in the rhizosphere of soybean plants in organic production. Varieties of plants of soy were grown using the biopreparation "Philazonit", which was developed in the company "Philazonit of Ukraine". The study determined the effect of phylazonite biopreparation on the mycobiome of the rhizosphere of soybean plants. The interaction of plants of soy varieties Kent and Suzir’ja with phytopathogenic micromycetes in conditions of the organic production in the Central Forest-Steppe of Ukraine (Research farm “Skvyrske” of Institute of Agroecology and Nature Management of the National Academy of Agrarian Sciences of Ukraine (IANM of the NAAS) was investigated. The number of phytopathogenic micromycetes in the rhizosphere of plants of different varieties of soy is determined depending on the variety and technology of its cultivation. It was established that the biopreparation Philazonit inhibits the formation of the number of phytopathogenic micromycetes in the rhizosphere of plants of the soy variety Suzir’ja and Kent during the growing season. It is proved that the representatives of genera: Alternaria, Fusarium, Penicillium, Aspergillus are dominated in the rhizosphere of plants of soy of both varieties. In the rhizospheres of plants of the soy Suzir’ja variety the representatives of genus Penicillium (32.8%) most often occurred. The smallest number of micromycetes were members of the genus Aspergillus (9.5%). In the rhizosphere of plants of the Kent variety dominated by representatives of the genera Alternaria and Fusarium (35.6% and 34.1% respectively). Representatives of the genus Penicillium were 15% and Aspergillus – 15.3% It is proved noted that during the studies there was a correlation between the development of micromycetes and the hydrothermal coefficient – in the flowering phase (r=0.8) and in the ripening phase (r =0.9) except for the germination phase

Evaluation of the quality composition of micromycetes in the air of the premises of stationary medical institutions

Introduction. Today, micromycetes are the most common pathogens found in residential and industrial premises and in the hospital environment. At the same time, mold fungi are the cause not only of allergic diseases but also of mycoses of various localization. Mold fungi are hazardous for patients in hospitals on long-term treatment and exposed to immunosuppression factors. Medical personnel who spent a long time in rooms with high contamination by micromycetes in the air can develop mycogenic sensitization. The aim of this work was to show the quantitative and qualitative composition of fungi surrounding the patient and the doctor in multidisciplinary hospitals. Materials and methods. With the help of a microbiological aspirator, air samples were taken in the premises of class B of surgical and therapeutic departments, followed by sowing on Saburo agar. The identified fungi were identified using classical methods and MALDI-ToF mass spectrometry using Microflex LT (Bruker). Results. In the course of the study, the assessment of fungal contamination of class B premises in treatment and prophylactic institutions was carried out to assess the qualitative and quantitative composition. Also, comparing the composition and number of micromycetes between the premises of departments of different profiles was carried out. A high level of contamination of micromycetes in medical premises of class B - 100% of cases was revealed in studies of objects of the hospital environment. The structure of the identified fungi was very diverse. In all air samples were isolated fungi genera Aspergillus, Penicillium, Mucor, Alternaria, and Ulcoladium. Spores of these fungi belonging to opportunistic or saprophytes are found in the air everywhere. Still, a significant increase in microbial load due to micromycetes can cause such typical allergic diseases as bronchial asthma, allergic skin dermatoses, and allergies of other locations. Conclusion. The study results revealed increased fungal contamination in healthcare facilities that require a more careful approach to compliance with the microclimate parameters.

The pathogenic mycobium in seeds of cultural plant varieties

The number phytopathogenic micromycetes is determined in the seeds of such cultivated varieties of cultural plant as soybeans, sunflowers, yellow mustard, buckwheat and thistle, which are grown by organic technology. It is shown that the number of micromycetes in the plant seeds differs significantly and ranges from 0.2 to 1.3 thousand NCO/g seeds. It depends on properties plants variety and hybrid, which are characterized by some certain set of physiological and biochemical characteristics that influences the formation seed mycobiome and it is quantitative composition. In the seeds varieties of these crops we identified such species of the phytopathogenic fungi as Alternaria alternate Fr., Fusarium oxysporum Schleht, Cladosporium herbarum Lket Gray, Botrytiscinerea Pers. Ex Fr., Ascochyta fagopyri Bres., Alternaria tenuissima (Kunze) Wiltshire, Aspergillus P. Micheliex Haller, Pénicillium Link. It was found that the species composition of phytopathogenic micromycetes in the seeds of soybean cultivar Kent and thistle Riches is much more diverse than the seeds other crops dominated by fungi A. alternate and F. oxysporum, which are characterized by different frequency of occurrence. These phytopathogenic fungi can cause plant diseases at different stages of ontogenesis, produce mycotoxins and reduce the quality of plant products. In the mycobiome of seeds buckwheat varieties Sophia, Sуn and sunflower hybrids Oscar and Oliver are dominated by fungi of the genus Pénicillium аnd Aspergillus (60–90%). The seeds affected by saprotrophic fungi can be reinfected during storage. This reduces its field and laboratory germination of the seeds. Saprotrophic fungi that dominate the seeds significantly impair its quality during storage and cause dangerous plant diseases in agrophytocenoses during the cultivation of this variety/hybrid. This requires the application of additional preventive protection measures of plants. Mycobioma mustard seeds of а yellow variety Ford represents a small number the phytopathogenic micromycetes. This may indicate its environmental safety in organic production. Thus, the analyzed research results indicate a significant differentiation in varieties species as to composition and number of phytopathogenic species of micromycetes in the seed mycobioma. Therefore, in order to avoid ecologic risks and biological contamination of agrocenoses, it is necessary to test the seeds, as on the density of the mycobiome and on the frequency of phytopathogenic micromycetes damage.

Comparative Study of the Rhizospheric Microflora of Sunflower Cultivars Helianthus annuus (Asteráceae, Magnoliópsida) Grown on Soils with Anthropogenic Polyelemental Anomalies

In a laboratory pot experiment, two mutant cultivars of sunflower (Helianthus annuus cv. r2p2 and Helianthus annuus cv. r5n1) were grown on soils with anthropogenic polyelemental anomalies and on a background control soil, and a comparative analysis of their rhizospheric microflora was carried out. The numbers of soil bacteria, actinomycetes and micromycetes, as well as the numbers of rhizospheric microorganisms resistant to Zn2+, Pb2+, and Cu2+ ions were estimated in the rhizosphere of sunflower cultivars. Quantitative changes in the sunflower rhizospheric microboceno-ses, formed under the influence of both the plant genotype and technogenic soil pollution, were revealed. A pronounced stimulation of the rhizospheric microorganisms of all groups studied was found when plants were cultivated on the technogenically contaminated soil from PJSC Kosogorsk Metallurgical Plant. In this case, cultivar differences were observed, namely: the maximal number of bacteria and actinomycetes was revealed in the rhizosphere of H. annuus cv. r2p2, whilst the maximal number of micromycetes was revealed in the rhizosphere of H. annuus cv. r5n1. An increased number of microorganisms resistant to lead ions was revealed in the rhizosphere of H. annuus cv. r2p2. The observed changes in the structure of rhizospheric microbial communities of the sunflower cultivars manifested in the stimulation of the growth and activity of soil microflora can be in demand for phytoremediation of technogenically contaminated soil.

Microbiocenosis characteristics of agrogenically altered acid brown forest soils in humid subtropics of Russia

Under conditions of perennial cropping of industrial tea crop on acidic brown forest soils of the Black Sea coast of Russia, the following were investigated: number of bacteria, of actinomycetes (mainly the Streptomyces genus) and of micromycetes (saccharomycetes), seasonal dynamics and functional activity of the microbial complex during period of 2008-2019. Stationary experiments were launched in various backgrounds of mineral fertilizer application. The experiment was accompanied by the use of generally accepted recommended techniques for microbiological studies. As follows from the analysis of the data obtained, it was established that the long treatment period of mineral fertilizers at doses from N200P60K50 to N600P180K150 in given soil-climatic conditions under the tea crop contributed to significant decrease in the number of actinomycetes and nitrogen fixing bacteria of the Beijerinckia genus vs. the control variant (without fertilizers). The most significant decrease in the number of micromycetes was revealed immediately after the application of mineral fertilizers at a dose of N600P180K150. After 2 months, the number of this group of soil microorganisms was built back. Under the impact of both medium and high doses of mineral fertilizers, the functional activity decrease of soils (CO2 emission and potential nitrogen fixation activity) was noted.

Effect of combined treatments and chemicalization agents on soil mycobiota in spring barley crops

The research was carried out in the Central Russian branch of the Federal Research Center named after I.V. Michurin ”. Soil samples for the study were taken from experimental plots of the Tambov Research Institute of Agriculture of the branch of the Federal State Budgetary Scientific Institution “FNTs im. I.V. Michurin ”. For the first time in the northeastern part of the Central chernozem region, research is being conducted to study the microflora and microfauna of typical chernozem. The aim of the study is the effect of combined treatments, as well as the system of fertilizers on the accumulation, structure of the complex of soil phytopathogenic and saprotrophic mycobiota, as well as the microscopic population of the soil in crops of spring barley. As a result of mycological studies, a complex of soil mycobiota was revealed and the dependence of phytopathogens and saprotrophs on elements of agricultural technology was shown. Dump-surface tillage to one degree or another contributed to stabilization of the antagonistic soil mycobiota of the soil. The optimal dose of mineral fertilizer against the background of combined soil treatments has been determined, which makes it possible to successfully restrain the number of micromycetes that cause root rot. It was noted that micromycetes of the genus Pythium were most often found in soils for all crops, less than other phytopathogens. The research results have proved the peculiarity of identifying structures by the improved method.

Soil health is the basis of organic agriculture

The biologization of agriculture is a topical issue both in Russia and in the world. The objective of the presented experiment was to confirm the positive effect biopreparations treatment of the fruit crops on the soil microbiota. As a result, in soil samples selected depend on the intensity of chemical fungicides application, from 1.15 x 104 to 1.23 x 104 CFU of micromycetes in one gram of absolutely dry soil were isolated. It was found that the largest amount of potentially pathogenic fungi was isolated in a soil sample cultivated using only chemical preparations - 20.5% of the total number of colonies. In the variant with the inclusion of biopreparation in the technology – from 7.2% to 11.0% of potentially pathogenic fungi of the total number of micromycetes. The most common among potentially pathogenic micromycetes were fungi of the genus Fusarium spp. Fungi of the genus Trichoderma were detected in all variants – not exceed 10% in the total number of micromycetes.

СОСТАВ ПОЧВЕННЫХ МИКРОМИЦЕТОВ ПРИ РАЗЛИЧНОЙ СИСТЕМЕ СОДЕРЖАНИЯ ПОЧВЫ НА ВИНОГРАДНЫХ НАСАЖДЕНИЯХ

The aim of the research was to study the composition of soil micromycetes with different systems of soil maintenance on vine plantations. The studies were carried out in the farms of Anapa district of Krasnodar Kray according to generally accepted methods in soil science and mycology. The scientific novelty lies in the insufficient knowledge of soil fungi of ampelocenoses under conditions of increased anthropogenic impact. As a result of research in 2019, it was found that the largest number of colony-forming units of micromycetes in the soil was observed in the variant with sodding. The number of КОЕ in 1 gram of absolutely dry soil in the variant with sodding in May exceeded the variant with black fallow by 5 times, and in August by 2 times. Species diversity was represented by 11 species belonging to 8 genera: Mucor, Trichoderma, Penicillium, Aspergillus, Fusarium, Alternaria, Cladosporium, Vertecillium. In the complex of the isolated micromycetes in the variant with sodding in the spring, the species of r. Trichoderma. Its frequency of occurrence was 80%. On the black fallow in this period, the dominant position was occupied by the species of the r. Penicillium (30.4%). In the summer period, in the variant with turfing, the species of the genera Fusarium, Alternaria, Cladosporium, Vertecillium were isolated with a higher frequency of occurrence, while the dominant position was occupied by species of the r. Penicillium (27.4%). In the variant with the system of soil maintenance, black fallow species dominated Penicillium and Aspergillus. The number of micromycetes in the soils of ampelocenoses depends on the system of soil maintenance and environmental conditions, while a regrouping of the species composition of fungi is observed.

Вплив мікроорганізмів з фунгіцидною та інсектицидною діями на біологічну активність ґрунту кореневої зони кукурудзи

Вплив мікроорганізмів з фунгіцидною та інсектицидною діями на біологічну активність ґрунту кореневої зони кукурудзи