Acid phosphatase and alkaline phosphatase active colonies of bacteria, isolated from forest soils, were stained. The activity of acid and alkaline phosphatase and other soil properties (the number of aerobic bacteria, basal respiration, the level of ammonification, the number of bacteria active in ammonification, the level of nitrification, the number of micromycetes) were compared with the number of bacteria belonging to the genus Micrococcus. Soil samples were taken from the following horizons: F-AO1 (fermentative), H-AO2 (humic), and A (basic). The soil samples were taken from beneath forest stands in the Izera Mountains (North Bohemia, Czech Republic). The number of acid phosphatase active colonies correlated positively with the number of alkaline phosphatase active colonies in the F-AO1 horizon, and there was a high, positive correlation between the former and the level of ammonification in the H-AO2 horizon. The number of alkaline phosphatase active colonies correlated positively with organic carbon, the number of ammonification bacteria, and the number of micromycetes in the H-AO2 horizon. The A horizon was almost biologically inactive. Neither acid nor alkaline phosphatase activities correlated positively with the number of phosphatase active colonies of bacteria.
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