Non-infectious Diseases Research Articles

Metabolic biomarkers of neonatal sepsis: identification using metabolomics combined with machine learning.

Sepsis is a common disease associated with neonatal and infant mortality, and for diagnosis, blood culture is currently the gold standard method, but it has a low positivity rate and requires more than 2days to develop. Meanwhile, unfortunately, the specific biomarkers for the early and timely diagnosis of sepsis in infants and for the determination of the severity of this disease are lacking in clinical practice. Samples from 18 sepsis infants with comorbidities, 25 sepsis infants without comorbidities, and 25 infants with noninfectious diseases were evaluated using a serum metabolomics approach based on liquid chromatography‒mass spectrometry (LC‒MS) technology. Differentially abundant metabolites were screened via multivariate statistical analysis. In addition, least absolute shrinkage and selection operator (LASSO) and support vector machine recursive feature elimination (SVM-RFE) analyses were conducted to identify the key metabolites in infants with sepsis and without infections. The random forest algorithm was applied to determine key differentially abundant metabolites between sepsis infants with and without comorbidities. Receiver operating characteristic (ROC) curves were generated for biomarker value testing. Finally, a metabolic pathway analysis was conducted to explore the metabolic and signaling pathways associated with the identified differentially abundant metabolites. A total of 189 metabolites exhibited significant differences between infectious infants and noninfectious infants, while 137 distinct metabolites exhibited differences between septic infants with and without comorbidities. After screening for the key differentially abundant metabolites using LASSO and SVM-RFE analyses, hexylamine, psychosine sulfate, LysoPC (18:1 (9Z)/0:0), 2,4,6-tribromophenol, and 25-cinnamoyl-vulgaroside were retained for the diagnosis of infant sepsis. ROC curve analysis revealed that the area under the curve (AUC) was 0.9200 for hexylamine, 0.9749 for psychosine sulfate, 0.9684 for LysoPC (18:1 (9Z)/0:0), 0.7405 for 2,4,6-tribromophenol, 0.8893 for 25-cinnamoyl-vulgaroside, and 1.000 for the combination of all metabolites. When the septic infants with comorbidities were compared to those without comorbidities, four endogenous metabolites with the greatest importance were identified using the random forest algorithm, namely, 12-oxo-20-trihydroxy-leukotriene B4, dihydrovaltrate, PA (8:0/12:0), and 2-heptanethiol. The ROC curve analysis of these four key differentially abundant metabolites revealed that the AUC was 1 for all four metabolites. Pathway analysis indicated that phenylalanine, tyrosine, and tryptophan biosynthesis, phenylalanine metabolism, and porphyrin metabolism play important roles in infant sepsis. Serum metabolite profiles were identified, and machine learning was applied to identify the key differentially abundant metabolites in septic infants with comorbidities, septic infants without comorbidities, and infants without infectious diseases. The findings obtained are expected to facilitate the early diagnosis of sepsis in infants and determine the severity of the disease.

Characteristics of the Cohort of Personnel of the Sublimate Production of the Siberian Chemical Plant Involved in Working with Plutonium-239

239Pu has the property of initiating radiogenic diseases in workers who come into contact with it during their professional activities. To study adverse medical and biological effects and to study in detail the potential consequences of plutonium exposure on the body of personnel at nuclear energy facilities, it is necessary to form a cohort of people involved in working with this isotope. The purpose of the study is to form and characterize a cohort of Siberian Chemical Plant sublimation production employees involved in working with 239Pu in the period 1953–2000. The source of information for forming the cohort was the database of the regional medical and dosimetric register of the population of closed administrative-territorial formation Seversk and the personnel of the Siberian Chemical Plant, containing personal, dosimetric, medical and biological information regarding all current and former employees of the Siberian Chemical Plant (about 65,000 people) since the founding of the enterprise till now. As part of this work, a cohort of sublimation production employees involved in working with 239Pu 9 in 1953–2000 was formed (1,052 people, 577 men and 475 women). The dosimetric, medical and biological characteristics of workers included in the sublimation production cohort are given. The created sublimation production cohort will make it possible to conduct comprehensive retrospective and prospective epidemiological studies to assess the morbidity and mortality of nuclear energy facilities employees, as well as to draw scientifically based conclusions regarding the role of 239Pu in the initiation of the most important socially significant non-infectious diseases (for example, malignant neoplasms, diseases of the circulatory system etc.) and mortality from these diseases. The scientific data obtained as a result of this study will make it possible to determine the main directions for organizing a system for assessing risks to the health of personnel at sites for the decommissioning of nuclear facilities and the formation of a set of measures aimed at improving the health-saving system of nuclear facilities employees and extending their working longevity.

Unveiling the Potential of Gut Microbiota: Promising Approaches to Treat Drug-Resistant Epilepsy

Introduction: Epilepsy is a chronic, non-infectious disease of the brain. Data shows that even 70 million people worldwide suffer from it and one-third of them fail to control it with anti-epileptic drugs. The gut microbiota has an impact on the course of the disease. The microbiota mediates between the gastrointestinal system and the central nervous system.Purpose: The aim of the work is to analyse how modification of the gut microbiota affects patients with drug-resistant epilepsy.Material and method: In December 2023, an extensive search was conducted and articles found in the Medline (PubMed) databases were selected by using the following keywords: drug-resistant epilepsy, gut microbiota, gut-brain axis, ketogenic diet, probiotics, faecal microbiota transplantation, to focus on recent developments and advancements in the field, the search was limited to articles published between 2017 and 2023.Conclusions: The intestinal microbiota in drug-resistant epilepsy (DRE) patients differs from that of healthy individuals or drug-sensitive epilepsy patients. Acknowledging the presence of the gut-brain axis and its impact on the central nervous system, we can view altering the flora as a potential therapeutic approach for DRE patients. The ways in which microbiota changes have been confirmed by research include: ketogenic diet, antibiotics, probiotics and fecal microbiota transplantation (FMT). Modifying the microbiota through these mentioned methods is all promising for treating DRE.

Selection of parental pairs when obtaining F1 hybrids of white cabbage with resistance to the tip burn of the inner leaves

Relevance. During the formation of cabbage heads, under certain conditions, white cabbage is damaged by a burn of the top of the inner leaves of the head, which abroad received the stable name “tip burn”. This disorder is interpreted as a physiological or non-infectious disease, the cause of which is a violation of calcium intake. The disease manifests itself inside the cabbage head, resulting from the interaction of environmental stress factors and the predisposed genotype. The most effective way to combat this disorder is to develop highly tolerant hybrids. This is especially true for the south of Russia, where the stress load on plants is maximum.Purpose of the study – to develop a principle for selecting parental pairs for developing hybrids that are resistant to the tip burn of the inner leaves of a cabbage head, and to identify promising hybrids. Materials and methods. In 2021-2022 in FSBSI "Federal Scientific Rice Centre", in the department of vegetable growing, 45 hybrid combinations obtained on the basis of 10 inbred lines were assessed under field conditions for the trait "burn damage to the inner leaves of the cabbage head." An analysis of the combining ability of lines according to Griffin, and an analysis of the inheritance of the trait according to Heiman were carried out. In 2023, a competitive test of promising hybrids was carried out.Results. In both years of research, 5 lines had negatively stable TCA for burn damage: Ten4270, Yas25p, Bs1f, Agr 82, 270Hn111. SCA of lines is more subject to changes from year to year. The effects of SCA in hybrids due to dominance, epistasis and overdominance in individual combinations significantly influence the manifestation of the trait in the direction of decrease or increase. The principle of selecting parental pairs to develop stable hybrids is based on a combination of lines with a negative SCA value; in some cases, it is permissible to include one line with an average SCA and low SCA. Analysis of genetic parameters according to Heiman confirms the prevalence of non-additive inheritance of the lesion; in unstable forms, the disorder is controlled by dominant polygenes and is highly heritable.

Borderline Lepromatous Leprosy Masquerading as Granuloma Annulare: A Case Report

Background: Leprosy is a chronic granulomatous disease caused by Mycobacterium Leprae. Leprosy is a great imitator with its various atypical, unusual and varying clinical presentations which can be confused with many infectious and non-infectious diseases. Case Report: A 46-year-old female presented to the dermatology department with complaints of painful raised lesions over face, upper back, upper limbs, lower legs and feet for 12 months. Clinically suspected as a case of Granuloma annulare. Punch biopsy was taken which revealed epidermis with dermis showing nodules of histiocytes and lymphocytes with grenz zone at the dermoepidermal junction. Acid fast stain and Fite Faraco stain was performed which revealed the presence of leprae bacilli with bacteriological index of 4+. Conclusion: Detailed history taking and correlating clinical picture with morphology is required to diagnose atypical presentations of leprosy. Early diagnosis helps to provide appropriate treatment and thus prevent from devastating complications.

Proteome Signature for Differential Diagnosis of Patients with Bilateral Lung Infiltrates

BackgroundThe differential diagnosis of bilateral lung infiltrates and prognosis prediction can be challenging for clinicians in the intensive care unit (ICU). We analyzed the proteome from bronchoalveolar lavage fluid (BALF) and determine their usefulness for evaluating the infectious causes and mortality associated with bilateral lung infiltrates.MethodsIn the ICU cohort, 136 patients with bilateral infiltrate on chest radiographs were selected, and bronchoscopy with bronchoalveolar lavage (BAL) was performed. Proteomics profiling of the exosomes in the BALF (n=20) was conducted to identify candidate protein biomarkers potentially associated with infection or mortality. The BAL samples (n=116) were used to measure the candidate biomarker levels.ResultsThe candidate biomarkers, CD20, CLIC4, SCFD1, and TAP1, were selected for the differential diagnosis of infection or mortality. The levels of CD20 were significantly elevated in patients with non-infectious causes compared to those with infectious causes (248.6±154.5versus177.6±150.9 ng·mL−1, p=0.014). The levels of CLIC4, SCFD1, and TAP1 did not differ between the two groups. As per the receiver operating characteristic analysis, CD20 was a significant predictor of non-infectious causes (area under curve, 0.668; 95% CI, 0.567–0.769; p=0.002; cut off value, 167.6 ng·mL−1; sensitivity, 74.1%; specificity, 63.2%). There were no significant differences in the concentrations of the biomarkers between survivors and non-survivors.ConclusionsOur results suggested that CD20 levels in BALF might be a useful biomarker for differentiating non-infectious and infectious diseases in patients with bilateral lung infiltrates.

Medical Rehabilitation and Health Resort Treatment at the Resort “Sernovodsk-Kavkazsky”

INTRODUCTION. Medical rehabilitation and health resort treatment are important parts of the health care system of the Chechen Republic, which are actively developing with high potential and competitiveness. Studies on the treatment of patients with chronic non-communicable diseases show the effectiveness of natural therapeutic resources and other innovative non-medication methods. AIM. To analyze and identify the most promising for the development of the Resort “Sernovodsk-Kavkazsky” directions of treatment of patients with chronic non-infectious diseases. MATERIALS AND METHODS. Data from information-analytical systems of the sanatorium-resort industry of the Russian Federation and the data of our own research were used for the analysis. RESULTS. The analysis and the main results confirming the effectiveness of treatment of patients with chronic non-infectious diseases with the use of natural healing resources and other innovative non-medication methods realized in the Resort “Sernovodsk-Kavkazsky” are presented. The most promising ways of development of health resort treatment and medical rehabilitation are determined. CONCLUSION. The increasing activity of unfavorable environmental factors, which negatively affect the functional state of the organism and the level of health of the population as a whole, require from the health care system continuous improvement, including in the sanatorium and resort industry.

Roles of noncoding RNA in allergic rhinitis.

Allergic rhinitis (AR) is one of the most common respiratory noninfectious diseases and chronic inflammatory diseases, the incidence of which has been increasing in recent years. The main pathological characteristics of AR are repeated inflammation, airway hyperreactivity, mucus hypersecretion, and reversible airway obstruction due to inflammatory cell response. AR occurrence is associated with various factors, including those of genetic and environmental origins. Noncoding RNAs (ncRNAs) are a group of RNA molecules that cannot be converted into polypeptides. The three main categories of ncRNAs include microRNAs (miRNAs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs). NcRNAs play a crucial role in controlling gene expression and contribute to the development of numerous human diseases. Articles are selected based on Pubmed's literature review and the author's personal knowledge. The largest and highest quality studies were included. The search selection is not standardized. Several recent studies have indicated the relationship of ncRNAs with the development of respiratory allergic diseases. NcRNAs, including miRNAs, lncRNAs, and circRNAs, are important gene expression regulatory factors. We review the expression and function of ncRNAs in AR, their role as disease biomarkers, and their prospective applicability in future research and clinically. We also discuss interactions between ncRNAs and their influence on AR comprehensively, these interactions are essential for determining the underlying pathological mechanisms further and discovering new drug therapeutic targets. NcRNAs can be used as biomarkers for early AR diagnosis, disease surveillance and prognosis assessment. Various categories of ncRNAs play distinct yet interconnected roles and actively contribute to intricate gene regulatory networks. They are also therapeutic targets and biomarkers in other allergic diseases. This article demonstrates ncRNAs have a wide range of applications in AR treatment. The database covers three key areas: miRNAs, lncRNAs, and circRNAs. Additionally, potential avenues for future research to facilitate the practical application of ncRNAs as therapeutic targets and biomarkers will be explore. With further research and technological development, ncRNAs may provide additional innovative, effective solutions for AR treatment.

Postnatal supplementation with alarmins S100a8/a9 ameliorates malnutrition-induced neonate enteropathy in mice.

Malnutrition is linked to 45% of global childhood mortality, however, the impact of maternal malnutrition on the child's health remains elusive. Previous studies suggested that maternal malnutrition does not affect breast milk composition. Yet, malnourished children often develop a so-called environmental enteropathy, assumed to be triggered by frequent pathogen uptake and unfavorable gut colonization. Here, we show in a murine model that maternal malnutrition induces a persistent inflammatory gut dysfunction in the offspring that establishes during nursing and does not recover after weaning onto standard diet. Early intestinal influx of neutrophils, impaired postnatal development of gut-regulatory functions, and expansion of Enterobacteriaceae were hallmarks of this enteropathy. This gut phenotype resembled those developing under deficient S100a8/a9-supply via breast milk, which is a known key factor for the postnatal development of gut homeostasis. We could confirm that S100a8/a9 is lacking in the breast milk of malnourished mothers and the offspring's intestine. Nutritional supply of S100a8 to neonates of malnourished mothers abrogated the aberrant development of gut mucosal immunity and microbiota colonization and protected them lifelong against severe enteric infections and non-infectious bowel diseases. S100a8 supplementation after birth might be a promising measure to counteract deleterious imprinting of gut immunity by maternal malnutrition.

Healthy nutrition – prevention of chronic non-communicable diseases. Overview of recommendations

The article provides an overview of the National Recommendations on healthy nutrition available in Europe, Asia (China), the USA and the Russian Federation, assesses the compliance of the presented guidelines with the principles of a healthy diet according to the recommendations of the World Health Organization, and analyzes their role in the prevention of the development of non-infectious diseases, including number of diseases of the cardiovascular system. A search of relevant literature for the review was conducted in PubMed (www.pubmed.gov) using English keywords (“national dietary guidelines”, “WHO diet”, “FBDG”, “dietary guidelines”, “cardiovascular factors”), the website of the National Project of the Russian Federation “Healthy Nutrition” (https://здоровое-питание.рф), eLIBRARY.RU (https://elibrary.ru/defaultx.asp) using keywords in Russian (“ESSE-RF”, “risk factors for non-infectious diseases”, “nutrition”). Publications published between 1995 and 2022 were considered. The review included the National Recommendations in force for 2023, as well as the results of cohort and multicenter studies, the purpose of which was to assess the impact of current recommendations on the development of traditional cardiovascular risk factors, associated clinical conditions (myocardial infarction, stroke, etc.), as well as the impact on general mortality. Publications with completely duplicate data and conclusions were excluded, and in case of partial overlap, both sources were indicated. As a result, a list was compiled of articles, newsletters, and links with up-to-date information on the National Guidelines.

The Prevalence Pediatric Patients Attending in A Tertiary care Hospital in Dhaka, Bangladesh

Introduction The incidence of skin conditions in young people typically varies between nations and within a single nation’s regions based on social, economic, racial, and environmental factors. A significant public health issue is the morbidity that skin diseases cause in children. There is a dearth of research on this pediatric issue in our nation, which is either centered on a condition, a community, or a particular population group. Objective To evaluate the dermatological complaints of the pediatric patients attending a dermatology outpatient department in a tertiary care hospital. Materials And Methods We retrospectively analyzed a total of 2472 pediatric patients who were in age range 0-18 years and came to the OPD of dermatology & venereology department of Ibn Sina Medical College Hospital, Dhaka, Bangladesh, over a period of 1½ year from January, 2020 to June, 2021. Data were collected on special proforma and analyzed with appropriate method. Results Among 2472 patients 1157 (46.8%) were male and 1315 (53.2%) were female and most of the patients belonged to the age group 12-18 year (52.6%). Male female ratio was 0.88:1. All diseases were broadly classified into noninfectious (71.2%) and infectious dermatoses (28.8%). Dermatitis (38.9%), infections (28.8%) and acne (14.44%) came out to be the top most common cause for OPD attendances. For the specific skin diseases, 20.1% were atopic dermatitis, 14.44% were acne, and 11.8 % were fungal infections. Atopic dermatitis was the most common condition below 12 years of age. In 12-18 years of age group, the most common condition was acne; females were more affected than males. Among the viral infections, wart was the most common (22.1%). Conclusion Atopic dermatitis was found to be the most common noninfectious disease and fungal infection was the most common infectious disease in all age groups and genders. Bangladesh Journal of Medical Science Vol. 23 No. 04 October’24 Page : 1054-1059

Research progress of memory T cells in the pathogenesis of allergic rhinitis

Allergic rhinitis（AR） is a non-infectious chronic inflammatory disease of the nasal mucosa mainly mediated by immunoglobulin E（IgE） in atopic individuals after exposure to allergens. T cells are the core cell population. In recent years, studies have shown that memory T cells play an important role in the development of allergic rhinitis. This article reviews the pathogenesis of memory T cells in allergic rhinitis, in order to further improve the pathogenesis of allergic rhinitis and provide theoretical basis and reference for subsequent clinical drug treatment.

Efficacy and Safety of a Standardized CPAP Protocol in the Delivery Room in Late Preterm Infants with Infectious and Non-Infectious Lung Diseases

The aim of this study was to evaluate the efficacy and safety of a standardized protocol of delivery room CPAP therapy in late preterm infants with acute neonatal respiratory failure (ARF) caused by various conditions.Material and methods. A retrospective comparative study of the efficacy of the standardized CPAP protocol in the cohorts of late preterm infants (34–36 weeks) was conducted at the Yekaterinburg Perinatal Center. The comparison group (C, N=256) included infants who received CPAP therapy in the delivery room during 12 months in 2020 before the introduction of the standardized protocol. The study group (S, N=169) included infants treated with standardized CPAP in April-December, 2022. The following subgroups were identified in groups C and S based on the cause of ARF: transient tachypnea of the newborn (TTN; C: N=100; S: N=89), respiratory distress syndrome (RDS; C: N=84; S: N=39), and congenital infection (CI; C: N=54; S: N=37). Other causes of ARF in groups C and S were found in 18 and 4 infants, respectively.Results. Switching to the standardized CPAP protocol reduced the duration of mechanical ventilation by an average of 24 h (P=0.013), the incidence of documented cerebral ischemia (CI) from 64.1% to 53.2% in all subgroups (P=0.022), the length of stay in the neonatal ward from 12 to 11 days (P=0.001), and the length of stay in the hospital from 16 to 14 days (P=0.001) as well as the incidence of CI in the STTN subgroup vs CTTN (38.2% vs. 61.0%, P=0.002). No significant differences were found in the RDS and CI subgroups. The frequency and duration of binasal CPAP and lung ventilation in the neonatal ICU did not differ between subgroups. Pneumothorax within the first 24 h occurred in one patient in group C and in two patients in group S (P=0.339), all of whom were diagnosed with congenital infection. No damage to the nasal passages was observed in any group. Conclusion. The use of a standardized protocol of CPAP therapy for neonates born after 35 weeks of gestation with respiratory failure of any etiology can significantly reduce the severity and duration of illness and should be considered as a basic respiratory strategy in the delivery room when indicated.

THE RESULTS OF THE COURSE ON MASTERING THE ANALYSIS OF INDICATORS OF FUTURE SPECIALISTS IN THE FORMATION OF A HEALTHY LIFESTYLE ((WHOQOL-BREF) KAZ)

It is stated that the relevance of the problem studied in this article depends on the result of the course designed to master the analysis of the indicators of future specialists in the formation of QOL ((WHOQOL-BREF) KAZ). According to the WHO, the countries of the world that followed a healthy lifestyle reached 86.9 years of life, 81.5 years for men, and 84.3 years on average. On the contrary, the death rate in the Republic of Kazakhstan in recent years was 29%, 6.77 per 1000 inhabitants. Mortality from diseases of the circulatory system was -32.7%, from cancer -7.9%, from injuries -5.3%.Due to these conditions, as a result of recent measures taken in the country, by 2025, the share of citizens living a healthy lifestyle will increase to 40%, life expectancy will increase to 75 years, non-infectious diseases will decrease to 7%, and medical rehabilitation services will increase to 75%. coverage is expected.It can be noted that the average life expectancy of people in our country is behind that of many countries in the world, and this situation needs to be studied fundamentally and applied in the economic, social and humanitarian direction in the Republic of Kazakhstan. It is necessary to find a solution to the problems of understanding the tasks of protecting the health, strengthening the body, and increasing the responsibility for improving and strengthening the health of the population. During the analysis of the results of the training program of the course, its goals and objectives are to improve the introduction and use of the KAZZ scale (WHOQOL-BREF) in the field of sports for students of higher educational institutions of the country and to form a model of the education system and the formation of a healthy lifestyle. to increase the competitiveness of teachers; to provide teachers with the necessary knowledge and methodological skills for effective formation of a healthy lifestyle among students; aimed at forming a model of healthy lifestyle formation among teachers and students of higher educational institutions of the country.

Results of a survey of medical students on key aspects of health preservation

Purpose: Study of the influence and assessment of risk factors for chronic non-infectious diseases in medical students through an online survey. Materials and methods: During the period from 03/19/24 to 04/08/24, an online survey was conducted at the Rostov State Medical University using the google platform. A group of 1232 people was formed, including students from 1st to 6th year of all faculties. The results of the survey were systematized and analyzed. Results: Вy analyzing the information received from respondents, several groups of risk factors for chronic NCDs were identified: 1. Sleep disturbances; 2. Indifference to compliance with healthy eating standards; 3. Susceptibility to chronic emotional stress. Conclusion: Based on the results of the study, risk factors for chronic non-diseases were identified among Rostov State Medical University students (the leading ones were: sleep disturbance, non-compliance with healthy eating standards, stress). It was also found that indicators such as physical activity and the presence of bad habits are not common risk factors in this group of respondents. From the above, we can conclude about the feasibility and importance of introducing preventive measures to increase the importance and significance of such aspects as healthy.

Synovial fluid D-lactate- a pathogen-specific biomarker for septic arthritis: a prospective multicenter study.

The performance of synovial fluid biomarker D-lactate to diagnose septic arthritis (SA) and differentiate it from crystal-induced arthritis (CA), other non-infectious rheumatic joint diseases (RD) and osteoarthrosis (OA) was evaluated. Consecutive adult patients undergoing synovial fluid aspiration due to joint pain were prospectively included in different German and Swiss centers. Synovial fluid was collected for culture, leukocyte count and differentiation, detection of crystals, and D-lactate concentration. Youden's J statistic was used to determine optimal D-lactate cut-off value on the receiver operating characteristic (ROC) curve by maximizing sensitivity and specificity. In total 231 patients were included. Thirty-nine patients had SA and 192 aseptic arthritis (56 patients with OA, 68 with CA, and 68 with RD). The median concentration of synovial fluid D-lactate was significantly higher in patients with SA than in those with OA, CA, and RD (p<0.0001, p<0.0001 and p<0.0001, respectively). The optimal cut-off of synovial fluid D-lactate to diagnose SA was 0.033 mmol/L with a sensitivity of 92.3 % and specificity of 85.4 % independent of previous antimicrobial treatment. Sensitivity and specificity of synovial fluid leukocyte count at a cut-off of 20,000 cells/µL was 81.1 % and 80.8 %, respectively. Synovial fluid D-lactate showed a high performance for diagnosing SA which was superior to synovial fluid leukocyte count. Given its high sensitivity and specificity, it serves as both an effective screening tool for SA and a differentiator between SA and RD, especially CA.

Association between sialic acid, sialidase activity and cholesterol of lipid-containing circulating immune complexes in the blood serum of patients with atherosclerosis

Atherosclerosis is the most common chronic non-infectious diseases, in the pathogenesis of which the accumulation of lipids in the subendothelial layer of the arteries and the local inflammatory reaction play a significant role. The source of lipid accumulation in the vascular wall is modified low-density lipoproteins. Desialylation is one of the known modifications that leads to the emergence of atherogenic properties in low-density lipoproteins. Enzymes that have sialidase activity circulate in human blood, i.e., the ability to cleave sialic acid from low-density lipoproteins. Desialylated low-density lipoproteins are autoantigens and induce the production of IgG autoantibodies, which form immune complexes with low-density lipoproteins, which aggravates the course of atherosclerosis. The purpose of the study was to establish associations between the levels of sialic acid in low-density lipoproteins, sialidase activity and the cholesterol content of lipid-containing circulating immune complexes in the blood serum of patients with atherosclerosis. Blood sera from patients with coronary heart disease were used as biological material to determine indicators of sialidase activity, cholesterol content of lipid-containing circulating immune complexes and sialic acid in low-density lipoproteins, which were isolated from blood serum. Serum samples were obtained from the laboratory of clinical biochemistry of the Institute of Clinical Cardiology, A.L. Myasnikov Federal State Budgetary Institution National Medical Research Center of Cardiology named after Academician E.I. Chazov as unutilized residues after performing routine biochemical tests. Fifty-one samples of blood serum and low-density lipoproteins isolated from it were analyzed. A significant positive relationship was revealed between the cholesterol content of circulating immune complexes and sialidase activity in the blood serum (r = 0.305 at p = 0.029). At the same time, no correlation was found between the content of sialic acid in low-density lipoproteins and sialidase activity in the blood serum, as well as between the cholesterol content of circulating immune complexes in the blood serum and sialic acid in low-density lipoproteins. It should be assumed that increased sialidase activity in the blood serum leads to the formation of desialylated immunogenic low-density lipoproteins with the subsequent appearance of autoantibodies and the formation of lipid-containing circulating immune complexes.

The effects of stress on gut virome: Implications on infectious disease and systemic disorders.

The role of gut microbiota in health and disease is being thoroughly examined in various contexts, with a specific focus on the bacterial fraction due to its significant abundance. However, despite their lower abundance, viruses within the gut microbiota are gaining recognition for their crucial role in shaping the structure and function of the intestinal microbiota, with significant effects on the host as a whole, particularly the immune system. Similarly, environmental factors such as stress are key in modulating the host immune system, which in turn influences the composition of the gut virome and neurological functions through the bidirectional communication of the gut-brain axis. In this context, alterations in the host immune system due to stress and/or dysbiosis of the gut virome are critical factors in the development of both infectious and noninfectious diseases. The molecular mechanisms and correlation patterns between microbial species are not yet fully understood. This literature review seeks to explore the interconnected relationship between stress and the gut virome, with a focus on how this interaction is influenced by the host's immune system. We also discuss how disturbances in this finely balanced system can lead to the onset and/or progression of diseases.

FDG PET/CT Findings of Rapidly Progressive Interstitial Lung Disease in a Patient With Anti-MDA5 Dermatomyositis.

Anti-MDA5 dermatomyositis is associated with an increased risk of developing rapidly progressive interstitial lung disease with a high mortality. We describe FDG PET/CT findings in a case of rapidly progressive noninfectious interstitial lung disease associated with anti-MDA5 dermatomyositis. The lung disease showed bilateral diffuse ground-glass opacities with diffuse heterogeneous FDG activity predominantly in middle and lower zones, mimicking infectious pneumonia. Familiarity with the imaging features of this rapidly progressive noninfectious interstitial lung disease can help to suggest the diagnosis and guide appropriate management.

The influence of physical activity on the level of chronic inflammation in health and in non-infectious diseases

In this review we discuss the effects of exercise on systemic inflammation. The influence of moderate continuous training on humoral immunity and cellular immunity is separately considered. Recent data on the molecular mechanisms of this influence, such as myokines secreted by skeletal muscles and adipokines secreted by adipocytes, are discussed. Clinical data are provided on the effect of moderate physical activity on common diseases — cardiovascular diseases, diabetes, cancer, Alzheimer’s disease and others.