nM For Dopamine Research Articles

Two Types of Subgroup-Valence Heteroatoms (PIII, TeIV) Synergistically Controlling Octa-CeIII-Encapsulated Heteropolyoxotungstate and Its Electrochemical Recognition Properties.

Rapid development of the synthetic chemistry of polyoxometalates (POMs) has greatly driven the generation of structurally variable innovative POM-based materials. Herein, we synthesized a novel PIII and TeIV synergistically controlling octa-CeIII-encapsulated heteropolyoxotungstate [H2N(CH3)2]11K2Na6H11[Ce8(CH3COO)2(HPIIIO3)2W8O20(H2O)12(B-β-TeW8O30)2(B-α-TeW8O31)4]·64H2O (1). Its distinctive anion skeleton [Ce8(CH3COO)2(HPIIIO3)2W8O20(H2O)12(B-β-TeW8O30)2(B-α-TeW8O31)4]30- is built by two tetra-vacancy [B-β-TeW8O30]8- and four tetra-vacancy [B-α-TeW8O31]10- moieties linked through an inorganic-organic hybrid [Ce8(CH3COO)2(HPIIIO3)2W8O20(H2O)12]26+ {Ce8P2W8} cluster core. Interestingly, {Ce8P2W8} is assembled from four [W2O11]10- groups and two [HPIIIO3]2- anions and eight Ce3+ ions. Besides, 1 was further composited with carboxylated multiwalled carbon nanotube (CMCN), resulting in a bi-component 1/CMCN nanocomposite. An electrochemical recognition platform (named as 1/CMCN/GCE) was built by modifying 1/CMCN on a glassy carbon electrode (GCE) for electrochemical detection of dopamine (DPA) at physiological pH (pH = 7.0). The findings have shown that 1/CMCN/GCE exhibits a good detection limit of 4.95 nM for DPA. This work provides considerable inspiration to promote innovative and rational structure designs of POM-based materials and expand their applications to electrochemical and biological detection fields.

A Porphyrin‐functionalized Graphene Oxide Nanocomposite for Simultaneous Detection of Dopamine and Uric Acid

AbstractCarbon nanomaterials combined with porphyrins having the similar electrocatalytic active center to heme protein are quite promising for high‐performance nonenzymatic electrochemical sensor. Herein, the catalytic tetra(4‐aminophenyl) porphyrin (TAPP) molecules are covalent immobilized on the conductive graphene oxide (GO) surface (TAPP@GO) by a simple one‐pot method. TAPP@GO as a novel electrochemical sensor successfully realized the sensitive detection of dopamine (DA) and uric acid (UA) in nM level with high stability and repeatability. The detection limit (LOD) for DA and UA is as low as 38 and 47 nM, and sensitivity up to 40.3 and 54.2 μA mM−1 in a wide detection range from 0.1 to 350 μM, respectively. Impressively, both DA and UA in the coexistence system can be simultaneously determined with the LOD of 51 and 64 nM for DA and UA, which is further confirmed by accurate determination of both in urine samples. This work represents not only the first example of electrochemical sensor based on organic porphyrin molecules covalent linked on GO surface, but more importantly provides an effective method for developing high‐performance sensing materials.

Designing of surface engineered Ytterbium oxide nanoparticles as effective electrochemical sensing platform for dopamine

The simple and well framed sensing podium for Dopamine (DA) is holding significant importance in early analysis of neurodegenerative syndromes. Here, a facile electrochemical biosensor of Ytterbium oxide nanoparticles (Yb2O3 NPs) functionalized with (3-mercaptopropyl)trimethoxysilane (MPTMS) were prepared for efficient and selective quantification of DA. The developed electrocatalytic sensor overcome the associated confronts related to selectivity, sensitivity and interference from co-existing bio-components. The fabrication of MPTMS@Yb2O3 NPs was carried out by hydrothermal route. The physiochemical, morphological and structural characterization of chosen electrocatalyst was performed HRTEM, FE-SEM, XRD, SAXS, Raman, FTIR, TGA-DTA, XPS and DSC techniques. The electrochemical investigation suggests the superior performance of MPTMS@Yb2O3 NPs modified gold electrode as compared to the individual MPTMS or Yb2O3 modified electrodes towards DA sensing. The formed sensor displayed wide linear ranges from 1 to 40 µM with lowest detection limit (LOD) of 41 nM for DA. The calculated diffusion coefficient value is 8.57 × 10−5 cm2s−1 for DA. The usability of Au/ MPTMS@Yb2O3 NPs for DA estimation was successfully checked in real samples for practical applications. The developed sensor offers a great signal recovery and reproducibility and showed enhanced prospective in clinical samples.

A highly stable copper nano cluster on nitrogen-doped graphene quantum dots for the simultaneous electrochemical sensing of dopamine, serotonin, and nicotine: a possible addiction scrutinizing strategy.

A highly stable copper (Cu) nanocluster (NC), which exhibited stability for more than one year, was synthesized using nitrogen-doped graphene quantum dots (N-GQDs) as reducing and capping agents and smaller glutathione molecules as additional capping agents. The synthesized NC, CuNC@N-GQDs, successfully sensed dopamine (DA), serotonin (SER), and nicotine (NIC) simultaneously with well-defined peaks and good peak-to-peak separation, whereas none of the controls including CuNCs and N-GQDs exhibited the simultaneous sensing properties. In addition, they exhibited enhanced sensitivity with current responses ∼4, ∼4, and ∼2 times those of the corresponding control for DA, SER, and NIC. The limits of detection obtained were 0.001, 1.00, and 0.01 nM for DA, SER, and NIC, respectively. The higher sensitivity and the simultaneous sensing are indicative of the synergistic effect of CuNCs and N-GQDs in the CuNC@N-GQDs. The sensing performance was successfully extended to real blood and urine samples spiked with DA, SER, and NIC.

Enzyme-like Fe-N5 single atom catalyst for simultaneous electrochemical detection of dopamine and uric acid

• Enzyme-like Fe-N 5 single atom catalyst was employed for efficient sensing application. • Highly sensitive simultaneous sensing of dopamine and uric acid was realized. • The corresponding biosensor has been successfully applied for real human serum sample detection. Simultaneous highly sensitive and accurate detection of dopamine (DA) and uric acid (UA) is of great importance since they are important biomolecules that are commonly coexisted in the human body and play a crucial role in numerous physiological and pathological features. In this work, Fe-N 5 single atom catalyst (SAC) supported on graphene was firstly employed for simultaneous electrochemical detection DA and UA. Owing to its unique electronic structure and abundant active sites, under optimal conditions, the enzyme-like Fe-N 5 SAC constructed electrochemical (bio)sensor exhibits excellent linear characteristic responses in the range of 0.005–500 and 0.01–480 µM, as well as the low limit of detection of 0.007 and 0.027 nM for DA and UA, respectively. More critically, the developed electrochemical system shows promising merits of exceptional stability, good repeatability, and, consequently, it was successfully applied for simultaneous sensitive detection of DA and UA in human serum samples.

Ferrocene‐Functionalized Multiwalled Carbon Nanotubes for the Simultaneous Determination of Dopamine, Uric Acid, and Xanthine

AbstractIn this proof‐of‐concept study, ferrocene‐modified multiwalled carbon nanotubes (Fc‐MWCNT) were synthesized by amide coupling and the resulting product was characterized by transmission electron microscopy and Fourier‐transform infrared spectroscopy. The Fc‐MWCNT were used in a graphite paste electrode (GPE) for the highly sensitive simultaneous determination of dopamine (DA), uric acid (UA), xanthine (XA) by differential pulse voltammetry. The sensor displayed a linear range up to 4.5, 14, and 82.0 μM with a limit of detection of 2.0, 10.0 and 5 nM for DA, UA, and XA, respectively. In addition, the modified GPE/Fc‐MWCNT displayed a high stability, reproducibility, and repeatability. The GPE/Fc‐MWCNT was used for simultaneous determination of DA, UA, and XA in two different real samples with a recovery rate of 95–104 %.

Nickel Cobaltite Nanoplate-Based Electrochemical Sensing Platform from Printable Inks for Simultaneous Detection of Dopamine and Uric Acid.

2D metal oxide-based nanomaterials have emerged as an exciting area of research owing to their rich electrochemical properties and diverse applications, including biosensors. In this work, we have synthesized ultra-thin Co3 O4 , NiO, and NiCo2 O4 nanostructures supported on a carbon cloth and printed graphite/Kapton substrates following thermal reduction of self-assembled metal alkanethiolates. These nanostructures act as a sensing platform for simultaneous detection of dopamine (DA) and uric acid (UA), important biological molecules in physiological and pathological tests. The ultrathin 2D nanoplates of NiCo2 O4 spinel formed in this study exhibit high electrochemical activity than pristine NiO and Co3 O4 . The electrochemical characterization studies indicate that NiCo2 O4 possesses a high potential for DA and UA with a peak separation of ∼140 mV, high sensitivity, and excellent selectivity. The low-cost and disposable, single-shot probe biosensors fabricated in this work possess a wide working range of 0.001-1000 μM with detection limits of 0.33 and 0.49 nM for DA and UA, respectively, with a practically achievable limit of quantification of ∼1 nM. Multiple sensing electrodes are printed on graphite/Kapton all at once following this method with improved reproducibility for DA and UA sensing further extending the scope of work towards mass fabrication and practical usage.

Construction of cationic polyfluorinated azobenzene/reduced graphene oxide for simultaneous determination of dopamine, uric acid and ascorbic acid

A highly sensitive cationic polyfluorinated azobenzene/reduced graphene oxide (C3F7-azo+/RGO) nanocomposite electrochemical sensor for simultaneous detection of dopamine (DA), ascorbic acid (AA) and uric acid (UA) was successfully synthesized using a facile exfoliation/restacking method. The nanocomposite is self-assembled from oppositely charged graphene oxide nanosheets (GO) and polyfluorinated azobenzene cations (C3F7-azo+), and then obtained by electrochemical reduction. The structure and electrochemical properties were characterized by X-ray diffraction (XRD), energy dispersive spectrometer analysis (EDS), transmission electron microscope (TEM) and scanning electron microscope (SEM). The electrochemical property of C3F7-azo+/RGO was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). It can be clearly seen from experimental results that C3F7-azo+/RGO-modified electrode (C3F7-azo+/RGO/GCE) can detect DA, AA and UA simultaneously, and has good stability and anti-interference performance. The detection limits are 65 nM, 8 nM and 11 nM for DA, AA and UA in the ranges 57.28–134.28 μM, 0.04–6.01 μM, 9.23–23.45 μM, respectively.

Efficient Sub-1 Minute Analysis of Selected Biomarker Catecholamines by Core-Shell Hydrophilic Interaction Liquid Chromatography (HILIC) with Nanomolar Detection at a Boron-Doped Diamond (BDD) Electrode

A rapid, sensitive method for the separation of catecholamine biomarkers (CAs), of importance in traumatic brain injury (TBI) and in Parkinson’s disease (PD), has been successfully developed using hydrophilic interaction liquid chromatography (HILIC). Dopamine (DA), epinephrine (EPI), and norepinephrine (NE) are known to be three to fivefold elevated above normal in traumatic brain injury (TBI) patients. HILIC facilitates the rapid and efficient separation of these polar biomarkers, which can be poorly retained by reversed-phase liquid chromatography (RPLC), while electrochemical detection (ECD) at the boron-doped diamond (BDD) electrode provides enhanced nanomolar detection. Three HILIC columns were compared, namely the superficially porous (core-shell) Z-HILIC column and the Z-cHILIC and Z-HILIC fully porous columns. The core-shell Z-HILIC showed the highest efficiency with a rapid separation within 60 s. The HILIC method utilizing the core-shell Z-HILIC column was initially optimized for the simultaneous analysis of DA, EPI, and NE using UV detection. The advantages of using the BDD electrode over UV detection were explored, and the improved limits of detection (LODs, S/N = 3) measured were 40, 50, and 50 nM for DA, EPI, and NE, respectively. Method validation is reported in terms of the linearity, repeatability, reproducibility, and LODs. Furthermore, the proposed method was successfully applied to the real sample analysis of urinary CAs following phenylboronic acid (PBA) solid phase extraction (SPE) pretreatment.

GdTiO3 perovskite modified graphene composite for electrochemical simultaneous sensing of Acetaminophen and Dopamine

A novel electrochemical biosensor was developed using GdTiO3 (GTO) perovskite prepared by sol-ge l(S) and hydrothermal (H) methods and decorated on few layered graphene for simultaneous sensing and quantification of dopamine (DA) and acetaminophen (ACE). The physical and structural characterization of the perovskites and composites were done using XRD, Raman, FTIR, XPS, and TEM analysis. Electrochemical characterization indicates higher activity for the GTO(S)-Gr composite modified electrode than the individual graphene, GTO(S) and GTO(H) component modified electrodes towards DA and ACE sensing. Simultaneous sensing in physiological buffer under optimized conditions exhibited wide linear ranges from 72 nM to 1.5 µM with lowest detection limit (LOD) 96.89 nM for DA and 50 nM to 1.5 µM with LOD 58.85 nM for ACE. The estimated sensitivity values are 3.357 × 10−5 A/nM and 2.177 × 10−5 A/nM, respectively for DA and ACE being higher than that of the literature reported for the graphene based metal oxide and perovskite sensors. The sensor showed high selectivity towards DA and ACE in the presence of co-interfering components like ascorbic acid, uric acid which may oxidize at the same potential.The binary composite was validated for DA and ACE sensing in practical applications using blood serum, tablet and urine samples and observed good signal recovery. The fabricated sensor was stable and showed good reproducibility.

Microplasma-Tunable Graphene Quantum Dots for Ultrasensitive and Selective Detection of Cancer and Neurotransmitter Biomarkers.

The effective and precise detection of cancer and neurotransmitter biomarkers including folic acid (FA), dopamine (DA), and epinephrine (EP) are essential for early detection and diagnosis of cancer and neurological disorders and for the development of new drugs. However, it remains challenging to detect FA, DA, and EP with high selectivity and sensitivity with a single material. Herein, we report a photoluminescence (PL)-based selective sensing of FA, DA, and EP with nitrogen-doped graphene quantum dots (NGQDs) synthesized from biocompatible chitosan under ambient conditions using atmospheric pressure microplasmas. By regulating the pH, the selective detection is achieved in broad ranges from 0.8 to 80 μM for FA and 0.4 to 100 μM for both DA and EP with the very low limits of detections of 81.7, 57.8, and 16.7 nM for FA, DA, and EP, respectively. The developed PL sensing method shows the high throughput of 5000 detections per hour. Moreover, highly stable colloidal NGQD dispersion with 100 μg/mL concentration for at least 100 PL detections is produced in 1 h by a single microplasma, and the process is scalable. The mechanisms of the outstanding performance are related to the enhanced, size-dependent π-π stacking attraction between the NGQDs and the pH-regulated chemical states of the analytes and the associated pH-specific photo-induced electron transfer and PL.

Screen-printed conductive carbon layers for dye-sensitized solar cells and electrochemical detection of dopamine

In the field of printed electronics, carbon and its allotropes are today among the most studied materials due to their unique physical and chemical properties. In this work, carbon dispersions were used for the preparation of screen-printed carbon electrodes applied as counter electrodes (CEs) for the dye-sensitized solar cells and as working electrodes for electrochemical sensing. Following the simple and quick homogenization process, carbon dispersions were subjected to thermogravimetric analysis to closely examine drying, eventually sintering processes after printing. The influence of the graphite:carbon black ratio was investigated. The structure of composite carbon layers was analyzed by scanning electron microscopy and optical microscopy. The DSSC CEs printed from the dispersion containing 100 wt% of CB exhibited the highest catalytic activity for the effective reduction of oxidized triiodide I3− back to iodide I− within the solar cell. The highest conversion efficiency achieved for the high-temperature processed CE was 3.05% with the fill factor of 0.65. The same composition of the carbon WE was used for the quantitative analysis of neurotransmitter dopamine. Based on the cyclic voltammetry measurements, the sensor showed a limit of detection of 13.3 nM for dopamine.

A UiO-66-NH2/carbon nanotube nanocomposite for simultaneous sensing of dopamine and acetaminophen

Carbon nanomaterials are quite promising to be combined with metal-organic frameworks (MOFs) to enhance the sensing ability of both materials. In this work, a MOF nanoparticle of UiO-66-NH2 is integrated with carbon nanotubes (CNTs) (UiO-66-NH2/CNTs) with a facile solvothermal method. The morphology, surface area and properties of this UiO-66-NH2/CNTs nanocomposite was investigated using electron microscopy, XRD, XPS, BET analysis and electrochemical techniques. Catalytic oxidation of dopamine (DA) and acetaminophen (AC) on this nanocomposite was achieved, owing to a 3D hybrid structure or a large electroactive surface area, excellent electrical conductivity, a large number of active sites of this nanocomposite. It was further utilized as a sensing platform to establish an electrochemical sensor for the monitoring of both DA and AC. The enhanced oxidation signals led to the voltametric sensing of DA and AC in a broad linear range from 0.03 to 2.0 μM and low detection limits (S/N = 3) of 15 and 9 nM for DA and AC, respectively. The proposed sensor also possessed good reproducibility, repeatability, long-term stability, selectivity, and satisfactory recovery in serum samples analysis. Therefore, it has the great potential for the accurate quantification of DA and AC in complex matrixes.

A Facile Fabrication of Poly‐ethionine Film on Glassy Carbon Electrode for Simultaneous and Sensitive Detection of Dopamine and Paracetamol

AbstractAn electrochemical sensor based on poly‐ethionine (Poly‐ET) film modified glassy carbon electrode was developed for sensitive and simultaneous sensing of dopamine (DA) and paracetamol (PA). The electropolymerization of ethionine monomer was carried out to modify the electrode. The modified electrode was characterized by using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The Poly‐ET/GCE exhibited excellent electrocatalysis towards the sensing of DA and PA. Poly‐ET/GCE showed a linear increase of current response with increase concentration of DA and PA ranging from 0.1 μM–60 μM and 0.1 μM–180 μM, respectively. The LODs were found to be 7 nM and 18 nM (S/N=3) for DA and PA, respectively. This electrochemical sensor was successfully utilized for the detection of DA and PA in pharmaceutical samples.

A Smartphone-Interfaced, Flexible Electrochemical Biosensor Based on Graphene Ink for Selective Detection of Dopamine

This study reports the fabrication of flexible electrochemical dopamine sensors using a facile, low temperature (300°C) process based on spin-coating of commercially available graphene ink onto a polyimide (PI) substrate. The electrochemical testing and surface characterization were achieved using cyclic voltammetry (CV), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). The graphene-ink based biosensor demonstrated a limit of detection (LoD) of 100 nM of dopamine in PBS and a dynamic concentration range up to 1 mM, with excellent specificity against uric acid and ascorbic acid. The sensor is also resilient against mechanical deformation ( $1~\mu \text{m}$ to 5 nM in artificial sweat. Wireless data transfer via Wi-Fi in tandem with an on-chip sensor integrated with an in-house built potentiostat is also developed to demonstrate the applicability of the platform for point-of-care applications.

Facile synthesis of large area pebble-like β-NaFeO2 perovskite for simultaneous sensing of dopamine, uric acid, xanthine and hypoxanthine in human blood.

Here, we report a facile one-step solid-state reaction assisted synthesis of β-NaFeO2 perovskite for simultaneous sensing of Dopamine (DA), Uric Acid (UA), Xanthine (Xn) and Hypoxanthine (Hxn) in human blood. The orthorhombic phase formation in β-NaFeO2 with the presence of octahedral sites is confirmed through x-ray diffraction (XRD) and Raman spectroscopy while high surface area pebble-like morphology is observed through scanning electron microscopy (SEM). The sensor exhibits distinct oxidation potentials for DA, UA, Xn and Hxn with a peak separation (ΔEp) between DA-UA, UA-Xn and Xn-Hxn as 134mV, 388mV and 360mV, respectively. The sensor exhibits an excellent selectivity, sensitivity and low limits of detection (LOD) of 2.12nM, 158nM, 129nM and 95nM for DA, UA, Xn and Hxn, respectively which are well below the lower limits of their presence in physiological ranges in human body fluids. The sensor shows an excellent selectivity and it was successfully employed in simultaneous sensing of DA, UA, Xn and Hxn in simulated blood serum samples with excellent recovery percentages. This is the first report on low-cost β-NaFeO2 modified GCE for simultaneous electrochemical sensing of biomolecules which can be applied for numerous bioanalytical applications.

Morphology-dependent MnO2/nitrogen-doped graphene nanocomposites for simultaneous detection of trace dopamine and uric acid.

Four nanostructured MnO2 with various controllable morphologies, including nanowires, nanorods, nanotubes and nanoflowers were synthesized, and then further composited with nitrogen-doped graphene (NG) with the assistance of ultrasonication. The surface morphologies, phase structures, and electrochemical performances of the proposed MnO2/NG nanohybrids were investigated by various techniques, and their catalytic activities on the electrooxidation of dopamine (DA) and uric acid (UA) were compared systematically. The sensing performances were found to be highly correlated with their morphologies. Among these morphologies, the nanoflower-like MnO2, composited with NG, displayed the most sensitive response signals for DA and UA. The boosted electrocatalytic activity was ascribed to the unique porous structure, large electroactive area, and low charge transfer resistance (Rct), which facilitated the electron transfer between electrode and analytes. Two linear response ranges (0.1μM-10μM and 10μM-100μM) were accompanied with very low detection limits of 34nM and 39nM for DA and UA, respectively. Moreover, the successful application of the MnO2NFs/NG composites for the simultaneous detection of DA and UA in human serum was realized using second-derivative linear sweep voltammetry (SDLSV). These findings give valuable insights for understanding the morphology-dependent sensing properties of MnO2 based nanomaterials, which is conducive to the rapid development of ubiquitous MnO2-based electrochemical sensors.

Determination of Fe3+ upon Special "Upconversion Luminescence" of Dopamine.

A promising technique based on the luminescence with long wavelength excitation and short wavelength emission (LExL, λex-L > λem) is developed. This LExL is different from traditional upconversion luminescence (UCL). The LExL, namely, special “UCL”, is realized by a xenon light source of a common spectrofluorometer. In this work, we found that dopamine (DA) has this LExL phenomenon. The LExL of DA is mainly caused by the excitations of second-order diffraction light (λex-L/2). The two-photon absorption properties of DA have been calculated employing the density functional response theory. The LExL and Stokes luminescence (SL, λex-S < λem) of DA both showed static quenching upon the addition of Fe3+. Dual-mode luminescence methods upon LExL (λex-L/λem at 565/317 nm) and SL (λex-S/λem at 282/317 nm) of DA were applied for the selective determination of Fe3+. The detection limits are 0.30 and 0.52 μmol L–1 for LExL and SL, respectively. In addition, their linear ranges for Fe3+ determination are both from 0.70 to 30 μmol L–1. The LExL method of DA not only meets the basic determination criteria for Fe3+ but also offers additional advantages in resisting more interferences and shows satisfactory feasibility performances.

Morphology-Dependent Electrochemical Sensing Properties of Iron Oxide-Graphene Oxide Nanohybrids for Dopamine and Uric Acid.

Various morphologies of iron oxide nanoparticles (Fe2O3 NPs), including cubic, thorhombic and discal shapes were synthesized by a facile meta-ion mediated hydrothermal route. To further improve the electrochemical sensing properties, discal Fe2O3 NPs with the highest electrocatalytic activity were coupled with graphene oxide (GO) nanosheets. The surface morphology, microstructures and electrochemical properties of the obtained Fe2O3 NPs and Fe2O3/GO nanohybrids were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. As expected, the electrochemical performances were found to be highly related to morphology. The discal Fe2O3 NPs coupled with GO showed remarkable electrocatalytic activity toward the oxidation of dopamine (DA) and uric acid (UA), due to their excellent synergistic effect. The electrochemical responses of both DA and UA were linear to their concentrations in the ranges of 0.02–10 μM and 10–100 μM, with very low limits of detection (LOD) of 3.2 nM and 2.5 nM for DA and UA, respectively. Moreover, the d-Fe2O3/GO nanohybrids showed good selectivity and reproducibility. The proposed d-Fe2O3/GO/GCE realized the simultaneous detection of DA and UA in human serum and urine samples with satisfactory recoveries.

Simultaneous and sensitive detection of ascorbic acid in presence of dopamine using MWCNTs-decorated cobalt (II) phthalocyanine modified GCE

The synthesis of tetra­8­[(E)­(4­methoxybenzylidene)amino] naphthalene­1­amine cobalt (II) phthalocyanine (CoTMBANAPc) from cobalt (II) tetracarboxylic acid phthalocyanine (CoTCAPc) via amide bridge has been described for the first time. These macromolecules displayed admirable solubility in aprotic organic solvents and compound has been characterized by UV–Vis, FT-IR, 1H NMR, XRD, TGA and Mass spectroscopic methods in addition to elemental analysis. The CoTMBANAPc was found to be electrochemically active and was immobilized on the GCE by decorating with MWCNT. The modified electrode was used for the simultaneous detection of two important biomolecules: Ascorbic acid (AA) and Dopamine (DA) by cyclic voltammetry, differential pulse voltammetry and amperometry methods. AA and DA exhibited two different and well-resolved cathodic peaks on the GCE/MWCNT-CoTMBANAPc electrode. The linear response was observed in the concentration range of 7.5 to 70 nM with detection limit of 6.6 μM and 0.33 nM for AA and DA, respectively on the modified electrode. The modified electrode is highly stable, sensitive and reproducible for the micromolar detection of AA and DA.