In real life, alliance organizations are common. Cross-border alliances and cross-industry cooperation are very popular business models where entities can achieve a win-win situation by leveraging each other’s strengths. To reveal how cooperation evolves under a cross-layer alliance, we introduce the prisoner’s dilemma game to depict the social dilemma, the interdependent two-layer network to depict the interaction structure. Eventually, the experimental results show that the alliance of individuals between layers can effectively inhibit network defective behavior and enhance the activity of cooperative behavior. As alliance strength increases, spontaneous symmetry breaking occurs and the breaking strength roughly tends to increase first and then decrease. This phenomenon is mainly caused by the asynchronous expansion of heterogeneous strategy coupling among two-layer networks, while the difference in time scales is another important factor in breaking the symmetry, thus further enriching the study of network reciprocity.
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