Net Surface Research Articles

The changing permafrost environment under desertification and the heat transfer mechanism in the Qinghai-Tibetan Plateau

With the development of desertification in the Qinghai-Tibet Plateau (QTP), aeolian sand becomes the remarkable local factor affecting the thermal state of permafrost along the Qinghai-Tibet Engineering Corridor (QTEC). In this study, a model experiment was conducted to analyze the impact of thickness and water content of aeolian sand on its thermal effect, and a hydro-thermo-vapor coupling model of frozen soil was carried out to reveal the heat transfer mechanism of the aeolian sand layer (ASL) with different thicknesses and its hydrothermal effect on permafrost. The results indicate that: (1) ASL with the thickness larger than 80 cm has the property of converting precipitation into soil water. The thicker the ASL, the more precipitation infiltrates and accumulates in the soil layer. (2) The cooling effect of ASL on permafrost results from the lower net surface radiation, causing the annual average surface heat flux shifting from heat inflow to heat outflow. The warming effect of ASL on permafrost results from the increasing convective heat accompanying the infiltrated precipitation. (3) As the ASL thickens, the thermal effect of ASL on permafrost gradually shifts from the cooling effect dominated by heat radiation and heat conduction to the warming effect dominated by precipitation infiltration and heat convection. The warming effect of thick ASL on permafrost requires a certain amount of years to manifest, and the critical thickness is suggested to be larger than 120 cm.

The emission, transport, and impacts of the extreme Saharan dust storm of 2015

Abstract. ​​​​​​​Each summer, the Saharan Air Layer (SAL) transports massive amounts of mineral dust across the Atlantic Ocean, affecting weather, climate, and public health over large areas. Despite the considerable impacts of African dust, the causes and impacts of extreme trans-Atlantic African dust events are not fully understood. The “Godzilla” trans-Atlantic dust event of 2020 has been extensively studied, but little is known about other similar events. Here, we examine the June 2015 event, the second strongest trans-Atlantic African dust event that occurred during the summers from 2003–2022. This event was characterized by moderately high dust emissions over western North Africa and an extremely high aerosol optical depth (AOD) over the tropical North Atlantic. The high dust loading over the Atlantic is associated with atmospheric circulation extremes similar to the Godzilla event. Both the African easterly jet (AEJ) and Caribbean low-level jet (CLLJ) have greatly intensified, along with a westward extension of the North Atlantic subtropical high (NASH), all of which favor the westward transport of African dust. The enhanced dust emissions are related to anomalously strong surface winds in dust source regions and reduced vegetation density and soil moisture across the northern Sahel. The dust plume reduced net surface shortwave radiation over the eastern tropical North Atlantic by about 25 W m−2 but increased net longwave flux by about 3 W m−2. In contrast to the Godzilla event, the 2015 event had minor air quality impacts on the US, partially due to the extremely intensified CLLJ that dispersed the dust plume towards the Pacific.

Methylglyoxal alters collagen fibril nanostiffness and surface potential

Collagen fibrils are fundamental to the mechanical strength and function of biological tissues. However, they are susceptible to changes from non-enzymatic glycation, resulting in the formation of advanced glycation end-products (AGEs) that are not reversible. AGEs accumulate with aging and disease and can adversely impact tissue mechanics and cell-ECM interactions. AGE-crosslinks have been related, on the one hand, to dysregulation of collagen fibril stiffness and damage and, on the other hand, to altered collagen net surface charge as well as impaired cell recognition sites. While prior studies using Kelvin probe force microscopy (KPFM) have shown the effect glycation has on collagen fibril surface potential (i.e., net charge), the combined effect on individual and isolated collagen fibril mechanics, hydration, and surface potential has not been documented. Here, we explore how methylglyoxal (MGO) treatment affects the mechanics and surface potential of individual and isolated collagen fibrils by utilizing atomic force microscopy (AFM) nanoindentation and KPFM. Our results reveal that MGO treatment significantly increases nanostiffness, alters surface potential, and modifies hydration characteristics at the collagen fibril level. These findings underscore the critical impact of AGEs on collagen fibril physicochemical properties, offering insights into pathophysiological mechanical and biochemical alterations with implications for cell mechanotransduction during aging and in diabetes. Statement of SignificanceCollagen fibrils are susceptible to glycation, the irreversible reaction of amino acids with sugars. Glycation affects the mechanical properties and surface chemistry of collagen fibrils with adverse alterations in biological tissue mechanics and cell-ECM interactions. Current research on glycation, at the level of individual collagen fibrils, is sparse and has focused either on collagen fibril mechanics, with contradicting evidence, or surface potential. Here, we utilized a multimodal approach combining Kelvin probe force (KPFM) and atomic force microscopy (AFM) to examine how methylglyoxal glycation induces structural, mechanical, and surface potential changes on the same individual and isolated collagen fibrils. This approach helps inform structure-function relationships at the level of individual collagen fibrils.

Unique myoglobin adaptation to endothermy and flight since the origin of birds.

Myoglobin (Mb) mediates oxygen diffusion and storage in muscle tissue and thus is important for the energy utilization and activity of animals. Birds generally have a high body temperature, and most species also possess the capability of powered flight. Both of these require high levels of aerobic metabolism. Within endothermic mammals, bats also independently evolved flight. Although the functional evolution of myoglobins in deep-diving amniote vertebrates has been well-studied, the functional evolution of myoglobin since the origins of both birds and bats is unclear. Here, with Mb-coding sequences from >200 extant amniote species, we reconstructed ancestral sequences to estimate the functional properties of myoglobin through amniote evolution. A dramatic change in net surface charge on myoglobin occurred during the origin of Aves, which might have been driven by positively selected amino acid substitutions that occurred on the lineage leading to all birds. However, in bats, no change in net surface charge occurred and instead, the Mb genes show evidence of strong purifying selection. The increased net surface charge on bird myoglobins implies an adaptation to flight-related endothermic and higher body temperatures, possibly by reducing harmful protein aggregations. Different from the findings of net surface charge, myoglobins of extant birds show lower stability compared with other amniotes, which probably accelerates the rate of oxygen utilization in muscles. In bats and other mammals, higher stability of Mb may be an alternative pathway for adaptation to endothermy, indicating divergent evolution of myoglobin in birds and bats.

Evaluation of some techniques for drying conidiated rice culture of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. with a bioassay evaluating their eligibility

BackgroundProduction of entomopathogenic fungi on a solid substrate, mostly on rice, is preferred by many producers due to its low technological requirements. Drying may be needed after fermentation to preserve the viability of the conidia until use. This study aimed to introduce some uncomplicated drying techniques and study their effectiveness in terms of drying time and extent, as well as their impact on the quantity of conidia produced and their biological activity. Four techniques were studied, i.e., condensing water vapor (by cooling), desiccant (using NaOH), air drying on a net, and air drying on a solid (impermeable) surface.ResultsCondensing water vapor and desiccant techniques were the fastest and most efficient, resulting in the lowest moisture content, ranging between 12 and 13%. While drying on a solid surface was the slowest and resulted in the highest moisture content of 32.98%. All of the dried samples yielded a lower quantity of conidia compared to the fresh samples. Samples of condensing water vapor, desiccant, and net techniques yielded approximately a similar quantity of conidia, ranging between 74 and 71% of the yield of the fresh sample. The solid surface sample yielded the lowest quantity of conidia, representing 63.78% of the fresh sample yield. A bioassay test on the mite, Tetranychus urticae Koch (Acari: Tetranychidae), showed that conidia dried via condensing water vapor and desiccant techniques kept their pathogenicity similar to the fresh sample, while samples dried on a net and solid surface significantly lost part of their pathogenicity.ConclusionThe condensing water vapor and desiccant techniques were the most efficient regarding the time needed, conidia yield, and pathogenicity. Drying on a solid surface was the least effective regarding the same factors.

The emission of CO from tropical rainforest soils

Abstract. Soil carbon monoxide (CO) fluxes represent a net balance between biological soil CO uptake and abiotic soil and (senescent) plant CO production. Studies largely from temperate and boreal forests indicate that soils serve as a net sink for CO, but uncertainty remains about the role of tropical rainforest soils to date. Here we report the first direct measurements of soil CO fluxes in a tropical rainforest and compare them with estimates of net ecosystem CO fluxes derived from accumulation of CO at night under stable atmospheric conditions. Furthermore, we used laboratory experiments to demonstrate the importance of temperature on net soil CO fluxes. Net soil surface CO fluxes ranged from −0.19 to 3.36 nmol m−2 s−1, averaging ∼1 nmol CO m−2 s−1. Fluxes varied with season and topographic location, with the highest fluxes measured in the dry season in a seasonally inundated valley. Ecosystem CO fluxes estimated from nocturnal canopy air profiles, which showed CO mixing ratios that consistently decreased with height, ranged between 0.3 and 2.0 nmol CO m−2 s−1. A canopy layer budget method, using the nocturnal increase in CO, estimated similar flux magnitudes (1.1 to 2.3 nmol CO m−2 s−1). In the wet season, a greater valley ecosystem CO production was observed in comparison to measured soil valley CO fluxes, suggesting a contribution of the valley stream to overall CO emissions. Laboratory incubations demonstrated a clear increase in CO production with temperature that was also observed in field fluxes, though high correlations between soil temperature and moisture limit our ability to interpret the field relationship. At a common temperature (25 °C), expected plateau and valley senescent-leaf CO production was small (0.012 and 0.002 nmol CO m−2 s−1) in comparison to expected soil material CO emissions (∼ 0.9 nmol CO m−2 s−1). Based on our field and laboratory observations, we expect that tropical rainforest ecosystems are a net source of CO, with thermal-degradation-induced soil emissions likely being the main contributor to ecosystem CO emissions. Extrapolating our first observation-based tropical rainforest soil emission estimate of ∼ 1 nmol m−2 s−1, global tropical rainforest soil emissions of ∼ 16.0 Tg CO yr−1 are estimated. Nevertheless, total ecosystem CO emissions might be higher, since valley streams and inundated areas might represent local CO emission hot spots. To further improve tropical forest ecosystem CO emission estimates, more in situ tropical forest soil and ecosystem CO flux measurements are essential.

High-resolution elevation models of Larsen B glaciers extracted from 1960s imagery

Accelerated warming since the 1950s has caused dramatic change to ice shelves and outlet glaciers on the Antarctic Peninsula. Long observational records of ice loss in Antarctica are rare but essential to accurately inform mass balance estimates of glaciers. Here, we use aerial images from 1968 to reveal glacier configurations in the Larsen B region. We use structure-from-motion photogrammetry to construct high-resolution (3.2 m at best) elevation models covering up to 91% of Jorum, Crane, Mapple, Melville and Flask Glaciers. The historical elevation models provide glacier geometries decades before the Larsen B Ice Shelf collapse in 2002, allowing the determination of pre-collapse and post-collapse elevation differences. Results confirm that these five tributary glaciers of the former Larsen B Ice Shelf were relatively stable between 1968 and 2001. However, the net surface elevation differences over grounded ice between 1968 and 2021 equate to 35.3 ± 1.2 Gt of ice loss related to dynamic changes after the ice shelf removal. Archived imagery is an underutilised resource in Antarctica and was crucial here to observe glacier geometry in high-resolution decades before significant changes to ice dynamics.

Photocatalytic mediated marine biofouling inhibition using nano CuO: TiO2-carbon dot embedded on organo silane surface modified polyethylene aquaculture cage nets

Biofouling in aquaculture cages is a potential problem and its management become major concern to the planners and farmers. The study aimed to modify the surface of polyethylene aquaculture cage nets using organo silane and to evaluate the effectiveness of a nano CuO:TiO2-carbon dot biocide treatment in inhibiting marine biofouling through photocatalytic action. Polyethylene aquaculture cage net surface modified using organo silane and the surface embedded with biocide of nano CuO:TiO2 and carbon dot (CD) derived from fish eye. The biocide-treated surface was characterized using UV–Visible and Fourier Transform Infrared spectrometry. The organo silane interacts with polyethylene via hydrogen bonding, CuO:TiO2 interacts with the silane's Si, and the carbon dots' conjugated CC bonds interact with the transition metal and silane through van der Waals electrostatic forces and hydrogen bonding. Varied concentrations of CuO:TiO2 and CD was coated sequentially over silane surface modified polyethylene, exposed in the marine environment to evaluate biofouling inhibition efficiency and found 0.05% each of CuO:TiO2 and CD was optimum. The CuO:TiO2–CD coated polyethylene cage net tested for its biofouling inhibition for 8 months in marine environment and exhibited excellent biofouling inhibition. The inhibition of biofouling was attributed to the enhanced photocatalytic action, resulting from increased electron-hole recombination, thus generating ROS, O2٠, and OH٠ radicals. This led to the highest electronic activity around the cage net and also the formation of an acidic environment deterred microorganisms. The study highlighted the use of organo silane for surface modification of polyalkenes to load the biocide and also CuO:TiO2–CD is a potential biocide for biofouling inhibition in aquaculture cages.

Heat the road again! Twenty years of surface urban heat island intensity (SUHII) evolution and forcings in 21 tropical metropolitan regions in Brazil from remote sensing analyses

This study analysed the influence of urbanisation on the expansion of surface urban heat island intensities (SUHIIs) and the variability of biophysical parameters in twenty one major Brazilian Metropolitan Regions (MRs), from 2003 to 2022. We use land surface temperature (LST) data from MODIS sensors to continuously quantify the daytime and night-time SUHIIs. Spectral reflectance and re-analysis data were used to access the variability of Enhanced Vegetation Index-2 (EVI2), surface albedo (α), urban area (UA) growth, net surface radiation (Rn), and actual evapotranspiration (ET). The Mann-Kendall trend test was applied to all variables and their relationships with LST were investigated using statistical metrics. The results show that the SUHIIs were 60 % (1.64 °C) higher during the daytime than during the night, with significant growth trends throughout the studied period and more pronounced monthly seasonal variations in higher latitude MRs. The UAs are shown to be more influential on LST during the daytime, with the association of LST with EVI2 and α playing a fundamental role in regulating the urban climate. The results also demonstrate that increasing ET potentially mitigates LST, especially during the daytime. For the night-time, Rn is a stronger factor influencing LST, leading to the higher availability of sensible heat.

Congo Basin Water Balance and Terrestrial Fluxes Inferred From Satellite Observations of the Isotopic Composition of Water Vapor

AbstractLarge spatio‐temporal gradients in the Congo basin vegetation and rainfall are observed. However, its water‐balance (evapotranspiration minus precipitation, or ET − P) is typically measured at basin‐scales, limited primarily by river‐discharge data, spatial resolution of terrestrial water storage measurements, and poorly constrained ET. We use observations of the isotopic composition of water vapor to quantify the spatio‐temporal variability of net surface water fluxes across the Congo Basin between 2003 and 2018. These data are calibrated at basin scale using satellite gravity and total Congo river discharge measurements and then used to estimate time‐varying ET − P over four quadrants representing the Congo Basin, providing first estimates of this kind for the region. We find that the multi‐year record, seasonality, and interannual variability of ET − P from both the isotopes and the gravity/river discharge based estimates are consistent. Additionally, we use precipitation and gravity‐based estimates with our water vapor isotope‐based ET − P to calculate time and space averaged ET and net river discharge within the Congo Basin. These quadrant‐scale moisture flux estimates indicate (a) substantial recycling of moisture in the Congo Basin (temporally and spatially averaged ET/P > 70%), consistent with models and visible light‐based ET estimates, and (b) net river outflow is largest in the Western Congo where there are more rivers and higher flow rates. Our results confirm the importance of ET in modulating the Congo water cycle relative to other water sources.

The joint effects of the El Niño-Southern Oscillation and Arctic Oscillation on tropical Indian Ocean heat flux during boreal winter

In this study, the joint effects of the El Niño-Southern Oscillation (ENSO) and Arctic Oscillation (AO) on winter net surface heat flux (Qnet) anomalies over the tropical Indian Ocean (TIO) are investigated for the 1979/1980–2017/2018 period. The results show that, in multisource datasets, the Qnet pattern for El Niño plus positive AO cases is the most robust. The major feature of Qnet is dominated by a significant dipole pattern, with oceanic heat gain anomalies appearing over the southeastern TIO and oceanic heat loss near the western TIO. Analysis of the flux components shows that the Qnet anomalies are mainly contributed by changes in latent heat flux and shortwave radiation (QLHF and QSWR), which are tightly associated with the regional atmospheric and oceanic conditions. In association with positive AO, northerly wind anomalies on the eastern flanks of a low-level anomalous anticyclone in the Arabian Sea enhance the intertropical convergence zone (ITCZ) in the western TIO. Meanwhile, the El Niño-related SST warming occurs in the western TIO. Both enhanced ITCZ and anomalous SST warming favour in situ increased low and middle cloud cover and a reduced QSWR. Furthermore, under El Niño's effect, a low-level anomalous anticyclone located in the southeastern TIO leads to a positive QLHF through decreased near-surface wind speed and increased near-surface air humidity. As a result, a dipole Qnet pattern tends to be observed for the combination of El Niño and positive AO.

Environmental impacts of aerosol radiative effect and urbanization and their interactions over the Beijing-Tianjin-Hebei City cluster

With fast economic development, urban expansion and air pollution are two main eco-environmental challenges confronting China. Though both are associated with intensified human activities, their impacts on urban environment are investigated separately, and the synthetic effect and intricate interaction of the two have not been fully understood. Here, based on in-situ measurements and online-coupled meteorology-chemistry modelling, the impacts of anthropogenic aerosol and urbanization are investigated simultaneously at Beijing-Tianjin-Hebei city cluster during a typical pollution season. The two processes exhibit opposite influences on meteorology and air quality. Aerosol via its radiative effect tends to cause net loss of solar energy with 3 °C cooling at the ground surface and 1 °C warming in the upper boundary layer. In contrast, urbanization yields large increment of net surface solar radiation as well as sensible heat flux, leading to over 1 °C warming in the lower atmosphere. Such modifications of boundary layer structure alter the accumulation and formation of air pollution. Urbanization effect dominated in the modification of near-surface air temperature and air pollution, making the synthetic effect a linear combination of two processes. The study highlights a comprehensive understanding of air pollution and urbanization and their synergy effect on urban environment over megacities.

The Inter‐Model Uncertainty of Projected Precipitation Change in Northern China: The Modulating Role of North Atlantic Sea Surface Temperature

AbstractPrecipitation changes in northern China are projected to increase in the Coupled Model Inter‐comparison Project Phase 6 (CMIP6) multi‐model ensemble. However, these projections are accompanied by notable inter‐model uncertainty, and the sources of this uncertainty remain largely unexplored. By analyzing 30 CMIP6 models, this research explores the source of inter‐model uncertainty in projected precipitation change and reveals the fundamental mechanism driving uncertainty spread. Following the empirical orthogonal function of inter‐model projected precipitation change, the leading mode displays a seesaw spatial pattern between northwest and north China. This phenomenon predominantly stems from the inter‐model divergence of projected sea surface temperature (SST) warming in the North Atlantic. Further scrutinizing the ocean mixed layer heat budget, we discover that the combined effect of surface sensible heat flux, net surface shortwave radiation flux, and ocean heat transport convergence influences heat flux and SST change of North Atlantic. The multi‐model projections indicate that localized increases in solar radiation and heat convergence warm sea surface, raising SST and initiating convective motion. This convective motion subsequently transforms the 200 hPa teleconnection wave train, leading to an anti‐phase pattern over northern China. This wave pattern modulates total cloud cover percentage, influences surface upward latent heat flux, and adjusts the top of atmosphere outgoing longwave radiation, collectively resulting in the seesaw pattern. Our study underscores the pivotal role of inter‐model disparities in North Atlantic SST warming projection, which is a primary driver of precipitation uncertainty in northern China. These insights offer an essential foundation for refining and diminishing inter‐model uncertainty.

Designing of SrFe2O4-decorated sulfur-MXene for super-fast adsorption of mercury

Needless to say, the severity of water pollution reached its peak, raising awareness to find a convenient strategy for improving this troublesome situation. Mercury (Hg2+) is listed as the 6th among the most jeopardous heavy metals. In this respect, MXene was double modified by; (i) Sulfur-doping to boost its affinity toward Hg2+, and then (ii) blending sulfur-doping MXene with the magnetic SrFe2O4 that could provide excellent recyclability and adsorption aptitude. Characterization analyses like XRD, XPS, and FTIR were applied to assure the well-fabrication of SrFe2O4/S-MXene and investigate its crystallinity, chemical composition, and function groups. The morphological structure of SrFe2O4/S-MXene was shown via SEM. Besides, the magnetization character and net surface charge were deduced by VSM and ZP measurements, respectively. Interestingly, it was observed superior adsorbability of SrFe2O4/S-MXene towards Hg2+ where qmax attained 838.99 mg/g during 15 min merely at pH 6 and 25 °C. Equilibrium isotherms elucidated the appropriateness of Freundlich and Langmuir to model the Hg2+ adsorption onto SrFe2O4/S-MXene. Additionally, kinetic analysis denoted that the adsorption data of Hg2+ obeyed the PSO. The adsorption mechanism of Hg2+ onto SrFe2O4/S-MXene was assumed to proceed by electrostatic interaction, surface complexation, and reduction reaction.

Pre-tension design and research of cable net structure for space modular deployable antenna

Modular deployable antennas represent an ideal structural form for the development of large-aperture antennas because of their flexibility, adaptability, and high versatility. To enhance the surface accuracy of the antenna after deployment, a comprehensive pre-tension design method that considers truss deformation and tension uniformity is proposed. First, the configuration design of the antenna cable net is constructed, and the mathematical models for cable length under surface accuracy requirements and boundary nodes are established considering catenary effects. Second, the distribution patterns of cable net structure nodes and segments are analyzed, leading to the creation of node coordinate matrices and cable net connection matrices. A basic model for cable net pre-tension design is developed based on the fundamental principles of force density. Furthermore, a multi-objective optimization of cable net pre-tension is performed using a genetic algorithm, considering truss structure deformation and tension uniformity as dual factors. Finally, the developed model is applied to design a single-module cable net structure, and numerical simulation is used for validation. Research results show that the overall surface form error is 0.32 mm, and the maximum tension ratio of cable net on the front cable net surface is 1.54, whereas the maximum tension ratio of tension ties is 2.28, thereby meeting the design requirements. Numerical simulation shows that the maximum deformation of the cable net structure is 0.16 mm, validating the correctness of the model. This research can provide valuable insights and references for the pre-tension design and research of cable net structures in other antennas.

Attributing the Decline of Evapotranspiration over the Asian Monsoon Region during the Period 1950–2014 in CMIP6 Models

Evapotranspiration (ET) accounts for over half of the moisture source of Asian monsoon rainfall, which has been significantly altered by anthropogenic forcings. However, how individual anthropogenic forcing affects the ET over monsoonal Asia is still elusive. In this study, we found a significant decline in ET over the Asian monsoon region during the period of 1950–2014 in Coupled Model Intercomparison Project Phase 6 (CMIP6) models. The attribution analysis suggests that anthropogenic aerosol forcing is the primary cause of the weakening in ET in the historical simulation, while it is only partially compensated by the strengthening effect from GHGs, although GHGs are the dominant forcings for surface temperature increase. The physical mechanisms responsible for ET changes are different between aerosol and GHG forcings. The increase in aerosol emissions enhances the reflection and scattering of the downward solar radiation, which decreases the net surface irradiance for ET. GHGs, on the one hand, increase the moisture capability of the atmosphere and, thus, the ensuing rainfall; on the other hand, they increase the ascending motion over the Indian subcontinent, leading to an increase in rainfall. Both processes are beneficial for an ET increase. The results from this study suggest that future changes in the land–water cycle may mainly rely on the aerosol emission policy rather than the carbon reduction policy.

Season‐Dependent Atmosphere‐Ocean Coupled Processes Driving SST Seasonality Changes in a Warmer Climate

AbstractAmplification of sea surface temperature (SST) seasonality in response to global warming is a robust feature in climate model projections but season‐dependent regional disparities in this amplification and the associated mechanisms are not well addressed. Here, by analyzing large ensemble simulations using Community Earth System Model version 2, we investigate detailed spatiotemporal characteristics of the amplification of SST seasonality focusing on the North Pacific and North Atlantic, where robust changes are projected to emerge around 2050 under SSP3‐7.0 scenario. Our results indicate that atmosphere‐ocean coupled processes shape regional changes in SST seasonality differently between warm (MAMJJAS) and cold seasons (ONDJF). During the warm season, the projected warming tendency is mainly due to increased net surface heat flux and weakening of vertical mixing. On the other hand, in the cold season, the projected cooling tendency is driven by strengthened vertical mixing over the North Pacific associated with the northward shift of storm tracks but weakened horizontal advection and mixing due to changes in ocean currents over the North Atlantic.

An induced mutation of ABC-transporter component VraF(K84E) contributes to vancomycin resistance and virulence in Staphylococcus aureus strain MW2

Staphylococcus aureus is a notorious pathogen responsible for various severe diseases. Due to the emergence of drug-resistant strains, the prevention and treatment of S. aureus infections have become increasingly challenging. Vancomycin is considered to be one of the last-resort drugs for treating most methicillin-resistant S. aureus (MRSA), so it is of great significance to further reveal the mechanism of vancomycin resistance. VraFG is one of the few important ABC (ATP-binding cassette) transporters in S. aureus that can form TCS (two-component systems)/ABC transporter modules. ABC transporters can couple the energy released from ATP hydrolysis to translocate solutes across the cell membrane. In this study, we obtained a strain with decreased vancomycin susceptibility after serial passaging and selection. Subsequently, whole-genome sequencing was performed on this laboratory-derived strain MWA2 and a novel single point mutation was discovered in vraF gene, leading to decreased sensitivity to vancomycin and daptomycin. Furthermore, the mutation reduces autolysis of S. aureus and downregulates the expression of lytM, isaA, and atlA. Additionally, we observed that the mutant has a less net negative surface charge than wild-type strain. We also noted an increase in the expression of the dlt operon and mprF gene, which are associated with cell surface charge and serve to hinder the binding of cationic peptides by promoting electrostatic repulsion. Moreover, this mutation has been shown to enhance hemolytic activity, expand subcutaneous abscesses, reflecting an increased virulence. This study confirms the impact of a point mutation of VraF on S. aureus antibiotic resistance and virulence, contributing to a broader understanding of ABC transporter function and providing new targets for treating S. aureus infections.

Green synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network

This study synthesized zinc oxide nanoparticles (ZnO NPs) using a novel green approach, with Sida acuta leaf extract as a capping and reducing agent to initiate nucleation and structure formation. The innovation of this study lies in demonstrating the originality of utilizing zinc oxide nanoparticles for antibacterial action, antioxidant potential, and catalytic degradation of Congo red dye. This unique approach harnesses eco-friendly methods to initiate nucleation and structure formation. The synthesized nanoparticles' structure and conformation were characterized using UV–vis (λmax = 280 nm), X-ray, atomic force microscopy, SEM, HR-TEM and FTIR. The antibacterial activity of the Nps was tested against Pseudomonas sp, Klebsiella sp, Staphylococcus aureus, and E. coli, demonstrating efficacy. The nanoparticles exhibited unique properties, with a crystallite size of 20 nm (XRD), a surface roughness of 2.5 nm (AFM), and a specific surface area of 60 m2/g (SEM). A Convolutional Neural Network (CNN) was effectively employed to accurately classify and analyze microscopic images of green-synthesized zinc oxide nanoparticles. This research revealed their exceptional antioxidant potential, with an average DPPH scavenging rate of 80% at a concentration of 0.05 mg/mL. Additionally, zeta potential measurements indicated a stable net negative surface charge of approximately −12.2 mV. These quantitative findings highlight the promising applications of green-synthesized ZnO NPs in healthcare, materials science, and environmental remediation. The ZnO nanoparticles exhibited catalytic capabilities for dye degradation, and the degradation rate was determined using UV spectroscopy. Key findings of the study encompass the green synthesis of versatile zinc oxide nanoparticles, demonstrating potent antibacterial action, antioxidant capabilities, and catalytic dye degradation potential. These nanoparticles offer multifaceted solutions with minimal environmental impact, addressing challenges in various fields, from healthcare to environmental remediation.

Rapid high-latitude cooling in the southeastern Pacific sector driven by North Atlantic warming during 1979–2013 in CESM1

During the 1979–2013 satellite observation period, the sea surface temperature (SST) has cooled substantially in the high-latitude Southern Ocean, with the most pronounced cooling tendency centered in the southeastern Pacific domain. Previous hypotheses have commonly ascribed the recent Southern Ocean cooling to either the tropical eastern Pacific cooling or North Atlantic and tropical Indian Ocean SST warming. However, the mechanisms underpinning significant cooling in the southeastern Pacific sector remain debatable. By diagnosing three pacemaker experiments with a state-of-the-art global climate model in which SSTs in the North Atlantic, tropical central-eastern Pacific, and tropical Indian Ocean-western Pacific are individually nudged to mimic the observed trajectory, we show that the North Atlantic dominates in the cold SST response of the southeastern Pacific sector during 1979–2013. Anomalous North Atlantic warming initiates a quasi-stationary Rossby wave response to a south-to-north cross-equatorial Hadley circulation strengthening, leading to an enhanced Amundsen Sea Low. As a result, due primarily to the increased low-level marine cloud cover, the net surface shortwave radiation reduction triggers rapid SST cooling in the southeastern Pacific domain. The southeastern Pacific cold SST anomalies are further maintained via the shortwave radiation-low-cloud-SST positive feedback on decadal timescales. Our results suggest that the shortwave radiation-low-cloud SST feedback is fundamental to the observed long-term cooling of the high-latitude Southern Ocean, with profound climate consequences worldwide.