Abstract Purpose: To evaluate the diagnostic ability of laser polarimeter GDx VCC to discriminate between healthy eyes, ocular hypertensive subjects and glaucoma patients. Methods: 417 eyes of 417 subjects were included. They were divided into 60 healthy controls, 289 ocular hypertensive subjects and 71 glaucoma patients. The GDx VCC parameters were compared among the study groups using analysis of variance. The receiver operating characteristic (ROC) curves were plotted for TSNIT average, superior average, inferior average, TSNIT standard deviation and nerve fiber indicator (NFI) between control and glaucoma eyes. Results: All evaluated parameters of the GDx VCC showed significant differences between control and glaucomatous eyes and between ocular hypertensive eyes and glaucoma patients. NFI had the greatest area under the ROC curve (AUC=0.881). The AUCs for the inferior average, TSNIT standard deviation and TSNIT average were 0,834, 0.824 and 0.819, respectively. NFI presented a larger AUC than the other GDx VCC parameters. Conclusions: The GDx VCC exhibited a good diagnostic ability to discriminate between healthy and glaucomatous eyes with damage in SAP. The best parameter was NFI.