Need For Homologous Blood Transfusion Research Articles

Postoperative Blood Salvage and Autotransfusion for Adult Spinal Deformity: A Randomized Controlled Trial.

Randomized controlled trial. The aim of this study was to determine whether postoperative blood salvage and autotransfusion versus traditional closed suction drainage reduces the rate of homologous blood transfusions in patients undergoing surgery for adult spinal deformity. The use of intraoperative blood salvage has become commonplace in spine surgery; however, the collection and reinfusion of blood drained from the wound postoperatively have not been employed routinely because of increased cost and questionable benefit. Adult patients undergoing long posterior fusions were randomized to either a blood salvage and reinfusion system (OrthoPat - Group 1) or a standard subfascial closed suction drain (Group 2). Blood collected in Group 1 was reinfused according to the manufacturer's protocol. Reinfusion drains were converted to standard closed suction drains when output was <50 mL during 4 hours, and drains were removed when output was minimal. Patients received autologous or homologous blood transfusions when hemoglobin (Hg) <8 g/dL or they had symptomatic anemia. Thirty-four patients were randomized into Group 1 and 36 patients into Group 2. There were no differences in preoperative or intraoperative parameters. Patients in Group 1 had higher hemoglobin levels on postoperative day (POD) 2 and POD 3 compared to those in Group 2. However, there was no significant difference in the percentage of patients requiring homologous blood transfusion between the two groups (41% Group 1 vs. 60% Group 2, P = 0.17). Similarly a subgroup analysis in patients with estimated blood loss >2000 mL also showed no difference in homologous blood transfusion rates (67% Group 1 vs. 76% Group 2, P = 0.58). There were no differences in the rate or type of postoperative complications. Postoperative blood salvage and reinfusion result in a higher hemoglobin level in the early postoperative period, but does not significantly reduce the need for homologous blood transfusion. 1.

Gravity Venous Drainage and the 3/8-Inch Venous Line: What Would Poiseuille Do?

The 1/2″ venous line has long been the drainage tubing diameter of choice for adult patients undergoing cardiac surgery. However, several programs use a smaller diameter venous line when used in conjunction with kinetic-assisted venous drainage or vacuum-assisted venous drainage. In 2014, our perfusion team made an institution-wide effort to miniaturize the cardiopulmonary bypass (CPB) circuit for children. One of our changes was the transition to a 3/8″ diameter venous line for drainage, even in our larger patients (up to 80 kg). We reviewed the current literature on this topic and delineated the various parameters required to be able to use the 3/8″ venous line with gravity drainage with the aim of using it on patients up to 115 kg with the appropriate venous reservoir. We have successfully used the 3/8″ venous line in more than 40 of our larger patients (35–90 kg) without the need for assisted venous drainage. We were able to reduce CPB prime from 625 ± 118 to 425 ± 52 mL before retrograde autologous priming (RAP)/venous autologous priming (VAP). The prime was further reduced to 325 ± 66 mL after RAP/VAP. Homologous blood utilization was reduced from 217 ± 311 mL to 27 ± 77 mL. Both results were statistically significant. We hypothesize that taking into account two of the parameters of Poiseuille’s law, namely length and diameter, it is possible to safely drain large children and mid-size adults via gravity venous drainage and the 3/8″ venous line. This technique allows reducing prime volume, simplifies CPB circuits with increased safety and potentially reduces the need for homologous blood transfusion.

Abstract PR017

Background: Jehovah’s Witnesses (JWs) do not accept blood transfusions of whole blood, although they may accept some blood plasma fractions at their own discretion. Members can specify which allowable fractions and treatments they will personally accept. Though some JWp may accept Normovolemic Hemodilution (NHD) if blood is attached to a return line that is connected to their body, our patient declined that alternative so we proposed Hypervolemic hemodilution (HHD). HHD means infusing extra fluid into the patient. Their circulating blood volume is thus increased, therefore they can afford to lose quite a lot more blood during surgery than they would otherwise. Case Report: 54y-o female, 60kg, with no comorbidity was scheduled to undergo total abdominal hysterectomy. She presented a Hb level of 12g/dl. Her baseline HR was 80 bpm, BP 120/70mmHg and SaO2 99% on room air. Anesthesia was induced with etomidate, fentanyl, cisatracurium, and maintained with sevoflurane, remifentanyle, cisatracurium. After induction we administered slowly 500cc Voluven and 1000cc Physiologic Serum. CAM 1´5-3 needed to keep controlled hypotension with a mean BP of 50-60mmHg. 60minutes after induction when HR was 70bpm, mean BP 55mmHg and SaO2 99%, the patient suffered a sudden ST segment depression in II,III,AVF (2mm) and tachycardia 105bpm. We suspected subendocardial injury caused by a decrease in the Hb level so we proceed to administer diuretics and decreased the CAM of sevofluorane. Results: 30minutes later, with 500ml diuresis and mean BP of 60mmHg, ST segment recovered normality. No increase of CPK & TroponineI were found and the patient had no chest pain after surgery. The patient presented a subendocardial injury due to intraoperative bleeding which was solved with hemoconcentration and decreasing the CAM of sevofluorane with no necessity of nitroglicerine or vasoactive drugs. Conclusions: HHD is an important technique that helps to avoid the need for homologous blood transfusions in JWs patients. We have to take into account the side effects of both hemodilution and hypervolemia: hemodiluting a patient risks acute anemia and hipoxemia. Hypervolemia risks pulmonary and cerebral edema, hyponatremia and congestive heart failure; therefore some relative contraindications for HHD are coronary and cerebral vascular disease, myocardial disfunction, hypertension and coagulopathy. Strict monitoring of fluid balance, diuresis, EKG, Sa02 and invasive blood pressure are mandatory.

Autologous bone plugs in unilateral total knee arthroplasty

Background:The purpose of this study was to compare blood loss, declines in hemoglobin (HgB) and hematocrit (HcT) levels, and required homologous transfusions for patients who either had the femoral intramedullary defect left open or filled with an autologous bone plug during total knee arthroplasty (TKA). We hereby present our results of autologous bone plugs in unilateral TKA.Materials and Methods:A retrospective chart review was performed on 55 patients diagnosed with osteoarthritis (OA) who had undergone unilateral TKA. Twenty six patients had the femoral defect filled with an autologous bone plug and 29 did not. Lateral releases and patella replacements were not performed. Drained blood was reinfused when appropriate.Results:Mean blood loss and mean blood reinfused were similar for the plugged (loss: 960.8 ± 417.3 ml; reinfused: 466.7 ± 435.9 mL) and unplugged groups (loss: 1065.9 ± 633.5 ml, P = 0.38; reinfused: 528.4 ± 464.8 ml, P = 0.61). Preoperative HgB (14.3 ± 1.4 g/dL, P = 0.93) and HcT levels (42.2 ± 4.6%, P = 0.85) were similar across plug conditions. HgB and HcT levels declined similarly for the plugged (2.7 ± 1.2 g/dl and 7.9 ± 4.0%) and unplugged groups (3.0 ± 0.9 g/dl, P = 0.16 and 9.0 ± 2.6%, P = 0.16), respectively. Of patients, one in the plugged group and none in the unplugged group required homologous transfusions (P = 0.5).Conclusion:The autologous bone plug does not appear to reduce the need for homologous blood transfusions following unilateral TKA.

Tranexamic acid reduces blood loss and financial cost in primary total hip and knee replacement surgery

Blood conservation strategies have been developed to diminish blood transfusion requirements in patients undergoing hip or knee replacement surgery. Tranexamic acid (TA) is an inexpensive antifibrinolytic agent that is little used in orthopaedic surgery due to the absence of standardised optimal administration regimens. Blood transfusion requirements and induced costs can be diminished by using TA according to a standardised administration protocol in a large cohort of patients. A retrospective study in patients who underwent joint replacement surgery by a single surgeon compared two periods, 2007-2008 without TA and 2008-2009 with TA. The 451 included patients underwent primary unilateral hip (n=261) or knee (n=190) replacement for osteoarthritis. Standardised protocols were used for surgery and anaesthesia. TA was given intravenously in a dose of 1g (i.e., 15mg/kg) at incision and wound closure then at 6-hour intervals for 24 hours. Blood losses were estimated using the Mercuriali formula. Haemoglobin on D -1 and D 8 and the number and volume of autologous (from intra-operative blood salvage) and homologous blood transfusions were collected. The costs of TA, blood salvage systems, and homologous blood units were recorded. The two groups were compared using Student's test, Wilcoxon's test, and the Khi(2) test, and multivariate analyses were performed. Values of p less than 0.05 were considered significant. TA use was associated with a significant decrease in the homologous blood transfusion rate (from 4% to 0%) and with 38% and 68% reductions in the rate and volume of autologous blood transfusions, respectively, due to a 34% decrease in blood losses. After taking into account the additional cost of TA therapy, there was a 25% reduction in the cost of the blood conservation strategy. TA therapy abolished the need for homologous blood transfusion and induced no notable side effects. TA therapy decreased the amount of blood salvaged intra-operatively, allowing a more rational use of the blood salvage system and decreasing the cost of anaesthesia. IV. Retrospective case-control.

Intraoperative autologous blood transfusion use during radical hysterectomy for cervical cancer: long-term follow-up of a prospective trial

A primary operative complication of radical hysterectomy for cervical cancer is hemorrhage. Intraoperative autologous blood transfusion (ABT) may be beneficial in reducing the need for homologous blood transfusion. Our institution published a prospective cohort study examining the use of ABT in cervical cancer patients undergoing radical hysterectomy in 1995. Patients who were initially consented to participate in this prospective trial using intraoperative ABT (cell saver) were evaluated with a median follow-up of 3years. We sought to update this original report with 16-year follow-up data collected from the clinical charts, Tumor Registry, and the Social Security Death Index. Two groups of patients undergoing radical hysterectomy were compared: patients who received ABT, and those who did not. Of the 71 original patients, all were included in this updated review, with an average follow-up of 12.4years for both groups. Originally, thirty-one patients received an ABT. In this group, 1 patient was lost to follow-up, and 4 (12.9%) are deceased including 1 (3%) with disease. In the non-autologous group, there were 7 (17.5%) patient deaths, including 3 (7.5%) with disease. Eighty-three percent were alive after 12years in both groups. The ABT group had 1 patient (3%) who developed a secondary malignancy, a colon adenocarcinoma. The non-autologous group had 2 patients (5%) who developed a secondary malignancy; one patient developed multiple myeloma and one patient developed a verrucous cancer of the tongue. Autologous blood transfusion during radical hysterectomy for cervical cancer appears safe and effective.

Effects of preoperative β-blocker on blood loss and blood transfusion during spinal surgeries with sodium nitroprusside-controlled hypotension

Background:The present study sought to determine whether premedication with oral β-blocker before hypotensive anesthesia with sodium nitroprusside could improve the quality of surgical field, decrease the blood loss, and decrease the need for homologous blood transfusion and duration of surgery.Methods:Eighty patients scheduled for spinal fixation surgery were included in a prospective, randomized, double-blinded study. Patients were classified into two groups: Group I received oral atenolol 50 mg twice one day before surgery; and Group II received placebo tablets identical in appearance to atenolol tablets for the same period and interval. All patients in both the groups received intraoperative sodium nitroprusside (SNP) as a hypotensive agent. Hemodynamic variables, amount of sodium nitroprusside used, quality of surgical field, and the amount of homologous blood transfusion and blood loss were compared between groups.Results:Heart rate and amount of SNP used were significantly less (P<0.0001) in the atenolol group, but no significant difference was found in intraoperative mean arterial blood pressure (MABP) between the two groups. The time of surgeries was significantly shorter in Group I than in Group II (185±15.21 vs 225±12.61 min), P<0.0001. The quality of surgical field was better in Group I than in Group II in all times of measurements, P<0.0001. The amount of blood loss and the amount of packed red blood cells transfused were significantly less in Group I than in Group II, P<0.0001. No clinically significant complications were observed in either group.Conclusion:Premedication with oral atenolol 50 mg twice/day for one day before hypotensive anesthesia with SNP during spinal surgeries seems to be clinically safe and effective to reduce heart rate, amount of SNP used, amount of blood loss, and amount of blood transfused with better quality of surgical field.

Blood conservation strategies for emergency open cardiac surgery in a patient with anti-M.

Blood conservation strategies for emergency open cardiac surgery in a patient with anti-M.

Time-related hemolysis in stored shed mediastinal blood after cardiopulmonary bypass

Reinfusion of mediastinal shed blood during cardiopulmonary bypass reportedly reduces the need for homologous blood transfusion. Although the fragility of blood components is thought to be amplified by shear stress during cardiopulmonary bypass and processing, the time-related deterioration of red blood cells (RBCs) in stored shed blood has not been studied extensively. In this study, we examined time-related hemolysis in shed blood stored at different temperatures. We examined processed shed blood collected from 15 patients (11 men and 4 women; mean age±standard deviation, 71±9years) during cardiopulmonary bypass. The shed blood was collected and stored at 20°C (group A) or 4°C (group B). Stored blood collected by venipuncture at the end of the surgery was used as a control. Damage was assessed by measuring its free hemoglobin (Hb) levels, using a photometric assay. The free Hb levels in blood samples from each group were tested at 0, 3, 6, 12, 24, 36, and 48h after surgery. The free Hb levels (g/dl) at 0, 12, and 24h were 0.03±0.01, 0.05±0.02*, and 0.06±0.02* in group A; 0.03±0.02, 0.04±0.03, and 0.05±0.02* in group B; and 0.01±0.01, 0.01±0.01, and 0.01±0.01 in the control group (*p<0.05 vs. 0h after surgery). The free Hb levels in stored shed blood significantly increased after 12h in group A (20°C) and increased after 24h in group B (4°C), whereas in drawn blood, they did not significantly increase over the first 24h. Compared to storage at 20°C, storage at 4°C suppresses the increase in the free Hb levels.

Cell saver use in obstetrics

This study is a review of our experience of using the cell saver in obstetrics. The main aim was to determine its role in decreasing the need for homologous blood transfusion. A retrospective study of patients identified as being at high risk of massive obstetric haemorrhage at the time of caesarean section during the period between July 2005 and August 2008, was undertaken. Cases were identified from our electronic database. A of 147 cases were identified. Cell saver was used in 77 (52%) of these cases. A totalof 13 units of salvaged blood were transfused successfully, saving approximately GB£1,800. Cell salvage in our practice has not been very effective due to non-availability of trained staff in emergencies and unfamiliarity of techniques, leading to poor salvage via suction. Most of our blood loss is swabbed not suctioned and lack of salvaging blood from swabs is a contributory factor.

A case of ovarian hemorrhage at early pregnancy managed by laparoscopic surgery with intraoperative autologous blood transfusion

We report a case of ovarian hemorrhage at 4 weeks of pregnancy that was managed with laparoscopic surgery with intraoperative autologous blood transfusion (IABT) and not homologous blood transfusion. We suspected ectopic pregnancy in a 37-year-old woman (para 2; postmenstrual day 32) because ultrasonography revealed massive hemoperitoneum and no gestational sac in the uterus, in spite of increased serum human chorionic gonadotropin (HCG) (4084 mIU/ml), and an emergency laparoscopic surgery was performed. Continuous bleeding from the surface of the left ovary was apparent. Corpus luteum and not chorion was detected; partial resection and coagulated hemostasis of the left ovary was performed. We aspirated 1350 ml of blood and reinfused 650 ml with IABT. Homologous blood transfusion was not required. Four days post-surgery (5 weeks of pregnancy), the gestational sac was detected in the uterus. Pathological examination revealed normal ovarian tissue with hemorrhage. The course of pregnancy was uneventful, and she delivered at 38 weeks of pregnancy. Although corpus luteum hemorrhage is commonly observed and safely followed without treatment, surgery is required for life-threatening hemorrhage. It is difficult to distinguish the findings of a ruptured corpus luteum without a detectable gestational sac at early pregnancy from ectopic pregnancy, and surgery is required for accurate diagnosis and hemostasis. Laparoscopic surgery with IABT avoids or reduces the need for homologous blood transfusion and is a safe and minimally invasive treatment for massive hemoperitoneum.

Präoperative Eigenblutspende und intraoperative Autotransfusion bei Erstimplantationen von Hüftendoprothesen - Eine retrospektive Studie

The records of a total of 102 patients who underwent primary total hip replacement during 1987-88 were evaluated retrospectively. 36 patients had donated 1-3 units of whole blood preoperatively. Surgery was performed with the use of a device for intraoperative autotransfusion (IAT). Another 25 patients without preoperative autologous blood donation were operated with the use of IAT. None of these techniques was available for the remaining 41 patients. The mean perioperative blood loss and the mean volume of transfused blood were 1400 ml and 1000 ml respectively. Equal quantities of blood were lost intra- and postoperatively, whereas only one fifth of transfused blood was given intraoperatively. Homologous blood transfusion was not required in 32% of the patients for whom autologous blood was not available. However after preoperative autologous blood donation, 95% of the 2-unit donors and 100% of the 3-unit donors could be operated without homologous blood transfusion. Autologous blood donation did not increase the need for homologous blood transfusions. With the use of IAT it was only 20% of the patients that at least 500 ml of blood were salvaged for retransfusion. On the basis of these findings, elective primary total hip replacement would seem to be the ideal operation to be performed after preoperative autologous blood donation and, whenever possible with the use of IAT. The desirable ratio of 1,5:1 for the units of blood to be crossmatched preoperatively vs. units transfused perioperatively can be achieved solely by preoperative donation of 3 units of autologous blood.

Effects of cardiopulmonary bypass circuit reduction and residual volume salvage on allogeneic transfusion requirements in infants undergoing cardiac surgery

Cardiopulmonary bypass in children may cause severe hemodilution and can lead to excessive perioperative blood loss and high transfusion requirements. Minimization of cardiopulmonary bypass circuit and salvage of red blood cells from the residual volume after the procedure are widely utilized to reduce allogeneic transfusion. We evaluated the effectiveness of those measures introduced in infant cardiac surgery in our institution. This retrospective observational study included 148 consecutive infants between 1 and 12 months of age, with a body weight <10 kg, who underwent an elective cardiac operation between 1997 and 2005. Patients were divided into three groups defined by the circuit prime volume; 700 ml (Group 1), 450 ml (Group 2) and 330 ml (Group 3). In Group 1 residual volume after perfusion was discarded and in Groups 2 and 3 was processed in a cell saving device. Analyzed variables were: perioperative blood loss, transfusion of homologous blood products and cell salvage product, and hematology data. Reduction of the circuit volume significantly diminished use of red blood cell concentrates from 1.6 units to 0.8 units (P<0.0001), and fresh frozen plasma from 1.3 units to 0.4 units (P<0.0001). Utilization of the cell salvage product reduced significantly (P=0.023) the postoperative need for homologous blood transfusion. Therefore, both measures proved to be effective in reducing homologous blood transfusion in infant cardiac surgery.

Homologous blood transfusion is not required in surgery for adolescent idiopathic scoliosis

We studied 70 consecutive patients with adolescent idiopathic scoliosis who underwent corrective surgery. They were divided into two groups. In the study group of 38 patients one or more modern blood-conservation measures was used peri-operatively. The 32 patients in the control group did not have these measures. Both groups were similar in regard to age, body-weight, the number of levels fused and the type of surgery. Only two patients in the study group were transfused with homologous blood and these transfusions were 'off-protocol'. Wastage of autologous pre-donated units was minimal (6 of 83 units). By contrast, all patients in the control group were transfused with homologous blood. In the study group there was a significant decrease (p = 0.005) in the estimated blood loss when all the blood-conservation methods were used. The use of blood-conservation measures, the lowering of the haemoglobin trigger for transfusion and the education of the entire team involved in the care of the patient can prevent the need for homologous blood transfusion in patients undergoing surgery for adolescent idiopathic scoliosis.

Impact of an Autotransfusion System during Endoluminal Repair of Abdominal Aortic Aneurysms: Does it Decrease the use of Allogeneic Blood?

Endoluminal repair of abdominal aortic aneurysms (AAA) is associated with decreased blood loss and transfusion requirements when compared to open AAA repair. We evaluated the use of an intraoperative autotransfusion system during endoluminal exclusion of AAAs. Fifty endoluminal AAA procedures selected at random were reviewed retrospectively. Patients were divided into 4 groups according to their estimated blood loss: Group I, 20 patients (0-500 cc); Group II, 18 patients (501-1,000 cc); Group III, 7 patients (1,001-1,500 cc); and Group IV, 5 patients (1,501-3,400 cc). The average blood loss was 327 cc for Group I, 728 cc for Group II, 1,217 for Group III, and 2,125, for Group IV. The overall blood loss was 834 cc, but 75 per cent was recovered. Hematuria was always present when greater than 1,000 cc of blood was retransfused, but renal function was not affected even with the concomitant administration of radiographic contrast. Only one patient required an autologous blood transfusion. The use of an autotransfusion device is safe, efficacious, and appears to further limit the need for homologous blood transfusion in patients undergoing endoluminal AAA repair.

Manejo perioperatorio de sangre en cirugía protésica primaria de rodilla

Preoperative autologous blood collection and the reinfusion of postoperative blood salvaged from wound drainage can help to decrease the use of homologous blood transfusion in total knee arthroplasty. We have evaluated 100 primary total knee arthroplasties carried out at the García Orcoyen Hospital between 2001-2003. Eighty-eight point seven percent (88.7%) of available autotransfusion blood packs were employed and the average blood reinfusion from wound drainage was 374 ml. (range: 200-950 ml) when this method was possible. In 69% of cases homologous blood transfusion was not necessary. While other techniques are being developed, we think that autologous blood collection and blood reinfusion from wound drainage are effective in reducing the need for homologous blood transfusion; this is important because of the potential risk of reactions, disease transmission and limited blood bank resources.

Randomized clinical trial of intraoperative autotransfusion in surgery for abdominal aortic aneurysm

Perioperative homologous blood transfusion (HBT) is associated with adverse reactions and risks transmission of infection. It has also been implicated as an immunosuppressive agent. Intraoperative autotransfusion (IAT) is a potential method of autologous transfusion. This was a single-centre randomized clinical trial of IAT in surgery for abdominal aortic aneurysm. Forty patients were randomized to IAT and 41 underwent surgery with HBT only. Patients in both groups received HBT to maintain haemoglobin levels above 8 g/dl. Transfusion requirements, and incidence of systemic inflammatory response syndrome (SIRS) and infection, were compared. Significantly fewer patients in the IAT group required HBT (21 versus 31; P = 0.038) and the median blood requirement per patient was 2 units lower (P = 0.012). There was a higher incidence of chest infection (12 versus four patients; P = 0.049) and SIRS (20 versus nine patients; P = 0.020) in the HBT group. Risk of SIRS was related to aortic cross-clamp time in the IAT group only. Use of autotransfusion effectively reduced the need for HBT and was associated with a reduced incidence of postoperative SIRS and infective complications.

Preoperative Acute Normovolaemic Hemodilution (ANH) in combination with Hypotensive Epidural Anaesthesia (HEA) during knee arthroplasty surgery. No effect on transfusion rate. A randomized controlled trial [ISRCTN87597684

BackgroundHypotensive epidural anaesthesia (HEA) combines a high epidural anaesthesia, performing a sympathetic blockade, with low-dose iv-infusion of epinephrine to stabilize circulation in the conscious patient. Mean artery blood pressure is reduced to 45–50 mmHg and hereby a reduced blood loss. In this study we have combined HEA with preoperative acute normovolaemic hemodilution (ANH) in attempt to further reduce the blood loss and need for blood transfusion in total knee arthroplasty surgery (TKR).MethodsTwenty-eight patients scheduled for TKR are randomised to ANH or no hemodilution (non-ANH). Both groups are anaesthetized with HEA. ANH is established with predonation of 20 % of the total blood volume, and replacement with equal volume of HAES 6 %. Blood re-transfusion is completed within 6 h.ResultsA mean of 877 ml blood was predonated (19.7 % of the total blood volume). Blood loss was, except from the intraoperative loss, significantly higher in ANH group. The total loss was 1306 mL (ANH) vs. 1026 mL (non-ANH), p < 0.05. Except from the first hour postoperatively, hematocrit was identical in between groups postoperatively. The amount of blood transfusion was identical 386 ml (ANH) vs. 343 ml (non-ANH) (ns). 50 % went through surgery without receiving blood (ANH) vs. 58 % (non-ANH). No renal, neurological or cardiopulmonary complications were registered.ConclusionsThese data suggest no benefits in combining HEA and ANH in TKR surgery. Probably because of the reduced viscosity of the blood after ANH, there is an increased postoperative blood loss. The need for homologous blood transfusion was identical.

Efficacy of preoperative donation of autologous blood in patients undergoing radical retropubic prostatectomy.

Radical retropubic prostatectomy (RRP) has resulted in substantial blood loss and the frequent need for homologous blood transfusion. In this study, the efficacy of autologous blood transfusion, from medical and financial perspectives, was evaluated in patients undergoing RRP. Between 1994 and 2000, 80 patients with localized prostate cancer underwent RRP in our institute. Based on informed consent, preoperative donation of autologous blood (PDA) was performed in 65 out of 80 patienets. Four or six units were donated during the first 3 years; however, donation units were reduced to a maximum of 4 units since 1997 onwards. The discard rate of donated blood and frequency of homologous transfusion were examined. Changes of hematocrit (Ht) and hemoglobin (Hb) levels through donation and surgery and important factors that may affect postoperative levels of Ht and Hb were evaluated in 56 patients receiving 4-unit donations. Overall, 2 or 4 units of donated blood were discarded in four patients and homologous transfusion was required in two patients. In 56 patients receiving 4-unit donation, the mean Ht level at predonation was 43.3%. Following donation, this decreased to 35.7%. The administration of recombinant human erythropoietin (rHuEpo) relieved declining Ht levels following donation, but changes in Ht levels after surgery were minor. Important factors related to postoperative decline of Ht and Hb levels were operative time and blood loss. The program of 4-unit PDA can be performed safely without rHuEpo injection, and it is useful to reduce the risk of requiring homologous transfusion. However, more efficient programs to relieve patient burden and to reduce medical cost are needed.

Compensatory increases in cardiac output and SMA blood flows prevent splanchnic hypoperfusion during moderate isovolemic hemodilution in dogs

Intraoperative acute isovolemic hemodilution has been used to decrease the need for homologous blood transfusion. Decreased arterial oxygen content and total oxygen delivery to tissues promote a compensatory increase in cardiac output, related to the hemodilution-induced decrease in viscosity and/or vasodilatation, preserving tissue oxygen delivery. Regional blood flow distribution may vary widely between and within organs. Splanchnic hypoperfusion, particularly at the intestinal mucosal region, has been implicated in systemic inflammatory response and multiple organ dysfunction. We evaluated systemic and splanchnic oxygen-derived variables during a moderate acute isovolemic hemodilution to test the hypothesis that hemodilution may promote gastric mucosal acidosis, despite an apparent adequacy of global markers of oxygen delivery and consumption.