Nardostachys Jatamansi Research Articles

Contact toxicity and repellent efficacy of Valerianaceae spp. to three stored-product insects and synergistic interactions between two major compounds camphene and bornyl acetate.

In this work, the essential oil (EO) and supercritical CO2 fluid extract (SF extract) of four Valerianaceae plants (Valeriana officinalis L., Valeriana officinalis L. var. latifolia Miq., Valeriana jatamansi Jones and Nardostachys chinensis Bat.) were chemically characterized. GC-MS analysis identified 74 compounds, representing 35.2%-82.4% of the total EOs and SF extracts. The EO was dominated by low-molecular-weight components while the SF extract was rich in fatty acids. Bornyl acetate and camphene were the characteristic compounds in EO and SF extracts. The efficacy of six extracts against three stored-product insects was investigated. In contact assays, V. officinalis exhibited strongest toxicity to red flour beetle (LD50=10.0 μg/adult), and V. jatamansi EO was the most active one against the cigarette beetle (LD50=17.6 μg/adult), while V. officinalis var. latifolia EO showed outstanding efficacy against the booklouse (LD50=40.2μg/cm2). Binary mixtures of two major compounds (camphene and bornyl acetate) were assessed for the contact toxicity to the red flour beetle. Additive effect existed in the natural proportion of V. officinalis, and synergism was observed in that of V. officinalis var. latifolia. This work confirmed the insecticidal efficacy of the species of the Valerianaceae family, and it would offer some information for the development of botanical insecticide.

Methyl jasmonate induced polyphenols and antioxidant production in callus suspension culture of Nardostachys jatamansi

In vitro establishment of callus suspension culture and incorporation of Methyl jasmonate (MeJA) was studied on the accumulation of polyphenols and other antioxidant activity in Nardostachys jatamansi. The maximum explants establishment (95%) was recorded in Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA 21.1 µM) and kinetin (Kn 3.16 µM). Leaf explants from in vitro derived shoots when cultured on MS medium in combination with NAA (23.1 µM), 6-Benzyladenine (BA 4.0 µM) and Kn (1.16 µM), 32% of the explants formed tiny adventitious roots with callus and 52% of the explants formed green callus without adventitious roots within 60 days of inoculation. Callus cultured with a combination of NAA, BA and Kn in ½ MS liquid medium exhibited maximum fresh weight (7.9 ± 0.10 g). Incorporation of MeJA in ½ MS liquid medium containing NAA, BA and Kn enhanced polyphenolic content and antioxidant activity, however it varied with the concentration used. MeJA-treated callus showed higher accumulation of all the measured polyphenols and antioxidant activity. This protocol is suitable to harness the industrial potential of the species without destroying plants growing in the natural habitat and this, in turn, would reduce collection from the wild.

In-Situ Conservation of Selected Medicinal Plant Species in Alpine zone Kandara Medicinal Plants Conservation Area in Uttarkashi, Uttarakhand

Among the 40 medicinal plant species of alpine zone for which Kandara MPCA has been declared for in-situ conservation only three globally significant medicinal plants viz. Aconitum heterophyllum (Endangered as per IUCN Red List), Picrorhiza kurrooa (Endangered) and Nardostachys jatamansi (Critically Endangered as per IUCN Red List) have been selected for in-situ conservation. The enrichment was standardized through planting seedlings, rhizomes and seeds from the natural populations in the MPCA at lower, middle and upper 1/3rd portions of the hill slopes in compartment 3b of Kandara MPCA. The upper 1/3rd portion of the hill slopes showed highest survival (52% seedlings and 42% from seeds) for Aconitum heterophyllum and 60% for seedlings, 42 % for rhizomes/stolons and 33 % for seeds of Picrorhiza kurrooa respectively in comparison to middle and lower 1/3rd portion of the hill slope. Seedlings performed better as compared to rhizomes and seeds in terms of survival percent. Therefore, enrichment planting through nursery raised seedlings can be an effective method for in-situ conservation of these medicinal plant species in their natural habitat.

Medicinal plants of the Bible\u2014revisited

BackgroundPrevious lists number from 55 to 176 plant species as “Biblical Medicinal Plants.” Modern studies attest that many names on these lists are no longer valid. This situation arose due to old mistranslations and/or mistakes in botanical identification. Many previously recognized Biblical plants are in no way related to the flora of the Bible lands. Accordingly, the list needs revision.MethodsWe re-examine the list of possible medicinal plants in the Bible based on new studies in Hebrew Biblical philology and etymology, new studies on the Egyptian and Mesopotamian medicinal use of plants, on ethnobotany and on archaeobotany.ResultsIn our survey, we suggest reducing this list to 45 plant species. Our contribution comprises 20 “newly” suggested Biblical Medicinal Plants. Only five species are mentioned directly as medicinal plants in the Bible: Fig (Ficus carica), Nard (Nardostachys jatamansi), Hyssop (Origanum syriacum), balm of Gilead (Commiphora gileadensis) and Mandrake (Mandragora officinarum). No fewer than 18 medicinal plants are mentioned in old Jewish post-Biblical sources, in addition to those in the Bible. Most of these plants (15) are known also in Egypt and Mesopotamia while three are from Egypt only. Seven of our suggested species are not mentioned in the Bible or in the Jewish post-Biblical literature but were recorded as medicinal plants from Egypt, as well as from Mesopotamia. It is quite logical to assume that they can be included as Biblical Medicinal Plants.ConclusionsAll our suggested Biblical Medicinal Plants are known as such in Ancient Egypt and/or Mesopotamia also. Examination of our list shows that all these plants have been in continuous medicinal use in the Middle East down the generations, as well as being used in the Holy Land today. Precisely in King Solomon’s words, “That which has been is what will be, that which is done is what will be done. And there is nothing new under the sun” (Ecclesiastes 1:9).

8α-Hydroxypinoresinol isolated from Nardostachys jatamansi ameliorates cerulein-induced acute pancreatitis through inhibition of NF-κB activation

Acute pancreatitis (AP) is a severe inflammatory condition of the pancreas, with no specific treatment available. We have previously reported that Nardostachys jatamansi (NJ) ameliorates cerulein-induced AP. However, the specific compound responsible for this inhibitory effect has not been identified. Therefore, in the present study, we focused on a single compound, 8α-hydroxypinoresinol (HP), from NJ. The aim of this study was to determine the effect of HP on the development of pancreatitis in mice and to explore the underlying mechanism(s). AP was induced by the injection of cerulein (50 μg/kg/h) for 6 h. HP (0.5, 5 or 10 mg/kg, i.p.) was administered 1 h prior to and 1, 3 or 5 h after the first cerulein injection, with vehicle- and DMSO-treated groups as controls. Blood samples were collected to determine serum levels of amylase, lipase, and cytokines. The pancreas was removed for morphological examination, myeloperoxidase (MPO) assays, cytokine assays, and assessment of nuclear factor (NF)-κB activation. The lungs were removed for morphological examination and MPO assays. Administration of HP dramatically improved pancreatic damage and pancreatitis-associated lung damage and also reduced amylase and lipase activities in serum. Moreover, administration of HP reduced the production of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in the pancreas and serum during AP. In addition, the administration of HP inhibited degradation of inhibitory κ-Bα (Iκ-Bα), NF-κB p65 translocation into nucleus and NF-κB binding activity in the pancreas. Our results suggest that HP exerted therapeutic effects on pancreatitis and these beneficial effects may be due to the inhibition of NF-κB activation.

Nardosinanone N suppresses LPS-induced macrophage activation by modulating the Nrf2 pathway and mPGES-1

The side effects of nonsteroidal anti-inflammatory drugs (NSAIDs) in the cardiovascular system mainly result from its inhibitory effect on cyclooxygenase-2 (COX-2). Since NSAIDs are one of the most commonly used anti-inflammatory drugs in the clinic, it is necessary to identify new anti-inflammatory drugs that are safer than NSAIDs. Nardosinanone N (NAN), a compound isolated from the roots and rhizomes of Nardostachys chinensis, was evaluated for its anti-inflammatory effects using the lipopolysaccharide (LPS)-stimulated RAW264.7 cell line and rat peritoneal macrophage models. First, we found that NAN down regulated the levels of nitric oxide (NO), inducible nitric oxide synthase (iNOS) and prostaglandin E2 (PGE2), but not cyclooxygenase-2 (COX-2). Additionally, NAN reduced the M1 macrophage phenotype and increased the M2 macrophage phenotype. Furthermore, mechanistic studies showed that NAN activated the nuclear factor-erythroid 2 -related factor 2 (Nrf2) signaling pathway, which, in turn, increased the expression of antioxidant protein heme oxygenase-1 (HO-1) to achieve its anti-inflammatory effect. Finally, Nrf2 siRNA and the HO-1 inhibitor significantly attenuated the anti-inflammatory effect of NAN. More interestingly, we found that NAN did not affect COX-2 expression and activity but reduced the PGE2 concentration by selective inhibition of microsomal prostaglandin E synthase-1 (mPGES-1). In conclusion, NAN may be a new anti-inflammatory drug that has fewer side effects than NSAIDs and can be a new potential Nrf2 activator and mPGES-1 inhibitor.

Nardostachys jatamansi (D.Don) DC.-Challenges and opportunities of harnessing the untapped medicinal plant from the Himalayas.

Nardostachys jatamansi (D.Don) DC. (family Caprifoliaceae) is prized in the Chinese, Tibetan, Nepalese, Bhutanese, Indian and Japanese systems of medicine. Its medicinal properties are well documented in ancient literature such as the Ayurvedic classics, the Old Testament, Ben-Cao- Shi-Yi, and Homer's Iliad. The plant is critically endangered and found in the alpine and sub-alpine regions of the Himalayas. Our study aims to show the challenges and opportunities of harnessing the untapped pharmaceutical resources of N. jatamansi. Another aim is to explore the possibilities of translating ethnobotanical information into health benefit applications. The sources of information used in the study are government reports, dissertations, books, research articles and databases like Science-Direct, SciFinder, Web of Science, PubMed, Wiley Online Library, and ACS Publications on N. jatamansi. In the study, we have examined and discussed reports on phytochemicals present in the plant, their structure, biological activity, pharmacological properties of extracts derived from plant parts, isolated compounds, and commercially available products from the plant. We have identified and discussed ambiguities and confusions about the plant's nomenclature and geographical distribution, and highlighted various studies that failed to discuss this issue. We analyzed the links between various reports on ethnobotanical information as well as studies on phytochemistry and pharmacologyconfirming the therapeutic properties of N. jatamansi. However, in many of these findings which had used dried samples, there was an uncanny resemblance between the phytochemical profiles and biological activities of N. jatamansi and Valeriana jatamansi Jones ex Roxb. (another genus from family, Caprifoliaceae). Since both the species share identical vernacular names, a possible reason could be that the samples may not have been of two separate species. Other limitations of different studies were the use of out-dated techniques for phytochemical profiling, absence of toxicology studies using animal models and clinical trials using human subjects. Analysis of various studies revealed a confusion about the botanical nomenclature of the plants and their geographical distributions. Absence of proper markers for identification of correct samples and improperly conducted studies on N. jatamansi were found to be the major hurdles to the use of ethnobotanical information and research findings into applications for human health. Development of markers using molecular, chemical and pharmacognosy based approaches for plant authentication and, in vitro propagation of authenticated material for easy availability of genuine plant material are the possible solutions to the problems identified.

Parkinson disease and Ayurveda

Parkinson’s disease, a degenerative brain disease of dopamine secreting brain cells Substantia nigra is increasing progressively and presents therapeutics -levodopa, carbidopa, MAOB inhibitor, COMT inhibitors, surgery and deep brain stimulations, though improve presenting feature but not quality of life. Present study using herbal composite containing Mucuna pruriens, Herpestis monnieri,Acors calamus, Nardostachys jatamansi and Withania somnifera taken in equal partshows better quality of life in all most all with marked improvement in movemement disorder in 92% cases as compared to 39% cases on conventional therapy. In addition Herbal composite also improve the haematological, hepatic and Renal function by bioregulating body biomechanics and neural cell function, revitalizing neural cell damage in substantia nigra check distruction of dopamine and facilitate optimal level of Dopamine for normal brain function.

Habitat characteristics of selected Medicinal Plants of Alpine and Sub Alpine Zone of Uttarakhand

The shrinking natural habitats due to unscientific harvesting, grazing pressure and climate change have severely threatened the medicinal plant resources. This paper presents the observation on the population, habitat and occurrence of 3 medicinal plants viz. Aconitum heterophyllum, Nardostachys jatamansi and Picrorhiza kurrooa in the Kandara and Khulia Medicinal Plant Conservation Areas (MPCAs) of Uttarakhand based on studies conducted by the author.

Nardostachys chinensis Batalin: A review of traditional uses, phytochemistry, and pharmacology.

Nardostachys chinensis Batalin (Valerianaceae) has been widely used in different traditional systems of medicine, including Islamic, Ayurvedic, Chinese, and Korean folk medicine. It has been used in traditional medicine as a tranquilizer, hepatotonic, cardiotonic, diuretic, and analgesic. Preliminary in vitro and in vivo studies have provided valuable scientific evidence for its traditional uses. This review aims to summarize reported traditional uses, phytochemistry, and pharmacological potential of N.chinensis while identifying potential areas of further research of plant. The review comprises literature pertaining to the pharmacological potential and phytochemistry of N.chinensis using worldwide accepted scientific databases via electronic search (Elsevier, Google Scholar, Pub Med, Scopus, Springer, Wiley online library). Moreover, data from ethno botanical text books available in library and electronic search were also included. The Plant List and Kew Herbarium Catalogue databases were used to authenticate the scientific name. Different pharmacological experiments in many in vitro and in vivo models have proved the potential of N.chinensis, namely, anti-inflammatory, anticonvulsant, antibacterial, antihypertensive, antifungal, neuroprotective, cardioprotective, aldose reductase inhibition, and antioxidant activities. The plant contains sesquiterpenenes of various varieties including aristolane, guaiane, and nardosinane types. Moreover, it also contains coumarins, phenols, lignans, neolignans, and glycosides. Reported activities suggested that there may be pharmacological potential for developing N.chinensis as a drug for infections, hypertension, cardiac diseases, Alzheimer's disease, insomnia, epilepsy, cancer, gastric, and liver diseases. More toxicological studies should be performed that will aid the progress to clinical trial studies of N.chinensis.

Parkinson Disease and Ayurveda

Parkinson’s disease, a degenerative brain disease of dopamine secreting brain cells Substantia nigra is increasing progressively and presents therapeutics-levodopa, carbidopa, MAOB inhibitor, COMT inhibitors, surgery and deep brain stimulations, though improve presenting feature but not quality of life. Present study using herbal composite containing Mucuna pruriens, Herpestis monnieri, Acors calamus, Nardostachys jatamansi and Withania somnifera taken in equal part shows better quality of life in all most all with marked improvement in movement disorder in 92% cases as compared to 39% cases on conventional therapy. In addition Herbal composite also improve the haematological, hepatic and Renal function by bioregulating body biomechanics and neural cell function, revitalizing neural cell damage in substantia nigra check destruction of dopamine and facilitate optimal level of Dopamine for normal brain function

Nardochinoid B Inhibited the Activation of RAW264.7 Macrophages Stimulated by Lipopolysaccharide through Activating the Nrf2/HO-1 Pathway.

Nardochinoid B (NAB) is a new compound isolated from Nardostachys chinensis. Although our previous study reported that the NAB suppressed the production of nitric oxide (NO) in lipopolysaccharide (LPS)-activated RAW264.7 cells, the specific mechanisms of anti-inflammatory action of NAB remains unknown. Thus, we examined the effects of NAB against LPS-induced inflammation. In this study, we found that NAB suppressed the LPS-induced inflammatory responses by restraining the expression of inducible nitric oxide synthase (iNOS) proteins and mRNA instead of cyclooxygenase-2 (COX-2) protein and mRNA in RAW264.7 cells, implying that NAB may have lower side effects compared with nonsteroidal anti-inflammatory drugs (NSAIDs). Besides, NAB upregulated the protein and mRNA expressions of heme oxygenase (HO)-1 when it exerted its anti-inflammatory effects. Also, NAB restrained the production of NO by increasing HO-1 expression in LPS-stimulated RAW264.7 cells. Thus, it is considered that the anti-inflammatory effect of NAB is associated with an induction of antioxidant protein HO-1, and thus NAB may be a potential HO-1 inducer for treating inflammatory diseases. Moreover, our study found that the inhibitory effect of NAB on NO is similar to that of the positive drug dexamethasone, suggesting that NAB has great potential for developing new drugs in treating inflammatory diseases.

Two isonardosinane-type sesquiterpenoids from Nardostachys jatamansi DC.

Two unique isonardosinane-type sesquiterpenoids, isonardosinonetriols A and B, together with six known sesquiterpenoids teucladiol, bullatantriol, (−)-globulol, (+)-maaliol, nardoguaianone J, and (+)-orobanchyl acetate, were isolated from the roots and rhizomes of Nardostachys jatamansi DC. (Caprifoliaceae). Their structures were elucidated by analysis of extensive spectroscopic data and the relative configurations were established by NOESY and circular dichroism (CD) experiments. Further, the results of SERT activity evaluations indicated that isonardosinonetriols A and B, (+)-maaliol and nardoguaianone J significantly enhanced SERT activity.

Liquid Chromatography-Tandem Mass Spectrometry of Desoxo-Narchinol a and Its Pharmacokinetics and Oral Bioavailability in Rats and Mice.

Desoxo-narchinol A is one of the major active constituents from Nardostachys jatamansi, which has been reported to possess various pharmacological activities, including anti-inflammatory, antioxidant, and anticonvulsant activity. A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of desoxo-narchinol A in two different biological matrices, i.e., rat plasma and mouse plasma, using sildenafil as an internal standard (IS). The method involved simple protein precipitation with acetonitrile and the analyte was separated by gradient elution using 100% acetonitrile and 0.1% formic acid in water as a mobile phase. The MS detection was performed with a turbo electrospray in positive ion mode. The lower limit of quantification was 10 ng/mL in both rat and mouse plasma. Intra- and inter-day accuracies were in the ranges of 97.23–104.54% in the rat plasma and 95.90–110.11% in the mouse plasma. The precisions were within 8.65% and 6.46% in the rat and mouse plasma, respectively. The method was applied to examine the pharmacokinetics of desoxo-narchinol A, and the oral bioavailability of desoxo-narchinol A was 18.1% in rats and 28.4% in mice. The present results may be useful for further preclinical and clinical studies of desoxo-narchinol A.

Simulating the effects of climate change across the geographical distribution of two medicinal plants in the genus Nardostachys.

BackgroundThe medicinal plants of Radix et Rhizoma Nardostachyos include Nardostachys jatamansi and N. chinensis. Traditionally, the two plants have been used to treat many diseases. Because of their special aroma, they are also commonly used in the food and cosmetics industry. Recently, N. jatamansi and N. chinensis have been overexploited due to their economic importance, resulting in a sharp decline in their wild resources. Predicting potential distributions of the genus Nardostachys under different climate scenarios and understanding its preferred habitat are of great significance for their conservation, artificial cultivation, and assessment of their value.MethodsThe Maxent model was used to predict the potential geographical distributions of the genus Nardostachys under current and future climatic conditions based on two representative concentration pathways (RCP2.6 and RCP8.5) for the 2050s and 2070s. These data were used to study the effects of climate variables.ResultsThe results show that the potential distribution of the two species will increase, thus more suitable habitats will be present in China. The suitable habitat for N. chinensis presents a relatively stable growth compared to N. jatamansi. In addition, precipitation plays a crucial role in modeling the effects of climate change on the genus Nardostachys. This study provides theoretical guidance for the cultivation of N. chinensis.

Aristolane-type Sesquiterpenoids from Nardostachys chinensis and Revised Structure of Aristolanhydride

Four hitherto unknown aristolane-type sesquiterpenes, including one novel 8,9-secoaristolane, namely secoaristolenedioic acid (1), two aristolone derivatives, namely 1α,2β-dihydroxyaristolone (2), 9-epidebilon (3), and one rare aristolane-chalcone hybrid, namely 3′-hydroxynardoaristolone A (4) were isolated from the ethanol extract of the roots and rhizomes of Nardostachys chinensis. Their structures were elucidated on the basis of extensive spectroscopic analysis. In addition, the structure of aristolanhydride, recently isolated from the same species, was corrected by reanalysis of the published NMR data.Graphical

Ethnomedicinal uses of plants for the treatment of nervous disorders at the herbal markets of Bojnord, North Khorasan Province, Iran.

Since ancient times, plants have been commonly used to cure human ailments by indigenous people throughout the world. Nervous diseases are rising all around the world. This paper provides important ethnobotanical information on plants that are used against neurological disorders and are available in medicinal markets of Bojnord, northeastern Iran. This survey, as an ethnobotanical study, was carried out between 2017 and 2018. The identification of the intended plant species was done using the available Floras. Some important indices such as the frequency of citations, relative frequency of citation (RFC), family importance value and use report were calculated for the medicinal plants included in the present study. The present study includes 58 medicinal plant species belonging to 36 families and reports their mentioning scientific and vernacular names, parts used, and preparation method. The most common family was Lamiaceae. The major parts of the identified plants used for treatment of nervous )neuropsychological( disorders were flower and aerial parts. The most common methods used for preparation of these plants were infusion and decoction. Stachys turcomanica, Tripleurospermum disciforme, Melissa officinalis, Nardostachys jatamansi, and Aloysiacitriodora had the highest rate of use report. Echium amoenum and Melissa officinalis had the highest RFC. The study indicated that although people in Bojnord have access to modern medicinal preparations, a considerable number of them still use medicinal plants for therapeutic purposes. Seemingly, most cited plants are worth more precise evaluations for their pharmacological activity.

Effect of "DXB-2030," a Polyherbal Formulation, on Experimental Polycystic Ovary Syndrome Associated with Hyperandrogenism.

The objective of the present study was to evaluate “DXB-2030,” a polyherbal combination of Trigonella foenum-graecum, Aloe vera, Sphaeranthus indicus, Nardostachys jatamansi, and Symplocos racemosa extracts in an experimental model of testosterone propionate (TP), induced polycystic ovary syndrome (PCOS) in female rats. Thirty animals were divided into 3 groups of 10 each; group 1 served as normal control; group 2 was administered with TP and served as positive control; along with TP, group 3 was treated with “DXB-2030” at a dose of 100 mg/kg p.o., for 60 days. At the end of the study period, the animals were subjected for the estimation of serum testosterone levels, oral glucose tolerance test (OGTT), weight of the ovaries, estrous cycle, and histopathological evaluation. An in vitro assay on GLUT4 expression was carried out to understand the effect of “DXB-2030” on insulin resistance. Results showed that treatment with “DXB-2030” reversed the TP-induced changes by increasing the GLUT4 expression and decreasing the body weight, testosterone levels, AUC of glucose in OGTT, and the cystic follicles of the ovaries, thus indicating its beneficial effect in PCOS by ameliorating the metabolic dysfunction and reproductive impairment, which are the pathophysiological conditions associated with PCOS. From the results obtained, it can be concluded that “DXB-2030” was effective in the management of experimental PCOS and hence may be recommended in the treatment of PCOS.

Reduction of Gamma Radiation-Induced Damage by Hydro-Alcoholic Extract of Nardostachys jatamansi.

The search for a nontoxic radioprotector has not yielded any promising results. Many antioxidant compounds, though effective under in vitro conditions as radioprotectors, have failed under in vivo settings due to their toxicity. The Indian medical system of Ayurveda uses a variety of plants with antioxidant potential, and these may be harboring molecules with radioprotective properties. In the present work, the radioprotective property of Nardostachys jatamansi was investigated. A hydro-alcoholic extract of this plant provided protection to the cellular DNA and membrane from 4 Gy gamma radiation. Depletion of cellular antioxidant status was also prevented by this extract. Molecular-level analysis in the intestines of mice revealed a lower bax/bcl2 ratio suggestive of a reduction of radiation-induced apoptosis. Expression levels of the DNA repair gene atm were elevated, along with a reduction in the expression of the inflammatory gene cox2. The extract also provided a survival advantage to mice exposed to lethal doses of gamma radiation. These results suggest a possible radioprotective role for Nardostachys jatamansi.

Narjatamanins A and B, a pair of novel epimers possessing a 2,3-seco-iridoid skeleton with an unusual 1,10-oxygen bridge from Nardostachys jatamansi and evaluation of their effects on worm paralysis in AD C. elegans.

Narjatamanins A (1) and B (2), a pair of epimers possessing a novel 2,3-seco-iridoid skeleton with an unusual 1,10-oxygen bridge, were isolated from the whole plants of Nardostachys jatamansi. Their structures were elucidated by a combination of various spectroscopic methods, including HRESIMS, IR and 1D and 2D NMR techniques. The absolute configurations of 1 and 2 were established by electronic circular dichroism (ECD) calculations. The pharmacological activities of 1 and 2 to alleviate AD-like symptoms were also evaluated using the Caenorhabditis elegans Alzheimer's disease (AD) pathological model, and narjatamanins A (1) and B (2) showed statistically significant delay in the worm paralysis phenotype of AD-like symptoms.