Abstract
Desoxo-narchinol A is one of the major active constituents from Nardostachys jatamansi, which has been reported to possess various pharmacological activities, including anti-inflammatory, antioxidant, and anticonvulsant activity. A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of desoxo-narchinol A in two different biological matrices, i.e., rat plasma and mouse plasma, using sildenafil as an internal standard (IS). The method involved simple protein precipitation with acetonitrile and the analyte was separated by gradient elution using 100% acetonitrile and 0.1% formic acid in water as a mobile phase. The MS detection was performed with a turbo electrospray in positive ion mode. The lower limit of quantification was 10 ng/mL in both rat and mouse plasma. Intra- and inter-day accuracies were in the ranges of 97.23–104.54% in the rat plasma and 95.90–110.11% in the mouse plasma. The precisions were within 8.65% and 6.46% in the rat and mouse plasma, respectively. The method was applied to examine the pharmacokinetics of desoxo-narchinol A, and the oral bioavailability of desoxo-narchinol A was 18.1% in rats and 28.4% in mice. The present results may be useful for further preclinical and clinical studies of desoxo-narchinol A.
Highlights
Nardostachys jatamansi is a pharmacologically versatile herb found in alpine Himalayas [1]
The roots of Nardostachys jatamansi have been used as an aromatic, bitter tonic, antispasmodic, stimulant, antiseptic, diuretic, and emmenagogue [2,3]
The pharmacological activities of Nardostachys jatamansi have been well demonstrated in experimental animals
Summary
Nardostachys jatamansi is a pharmacologically versatile herb found in alpine Himalayas [1].Traditionally, the roots of Nardostachys jatamansi have been used as an aromatic, bitter tonic, antispasmodic, stimulant, antiseptic, diuretic, and emmenagogue [2,3]. Nardostachys jatamansi is a pharmacologically versatile herb found in alpine Himalayas [1]. The pharmacological activities of Nardostachys jatamansi have been well demonstrated in experimental animals. Studies have reported that Nardostachys jatamansi possesses hepato-protective [1], gamma-aminobutyric acid (GABA) enhancing [4], anti-parkinsonian [5], and anticonvulsant [6] activities in rats. Various activities of Nardostachys jatamansi, including antidepressant [7], improvement of learning and memory [8], antidiabetic [9], and anti-inflammatory [10], have been demonstrated in mice. In-vitro-derived plants of Nardostachys jatamansi have shown an anti-cholinesterases, anti-hyperglycemic, anti-inflammatory, anti-hypertensive, and anti-tyrosinase potential [11]
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