Objectives: Compare the tensile strength and rupture elongation of room temperature vulcanizing silicone (RTV), heat temperature vulcanizing silicone (HTV) and 3% SiO2 reinforced RTV and HTV following disinfection with various agents. Materials and Methods: According to ASTM D412, 384 samples were fabricated using HTV, RTV, RTV and HTV reinforced with 3% SiO2 nanoparticles. The control group received no disinfection treatment, while the other samples were disinfected for 10 minutes using neutral soap, 4% chlorhexidine, and ozone water, three times a day for 60 days. Additionally, accelerated aging was carried out for 252,504,1008 hours. Tensile strength and rupture elongation were assessed using a universal testing machine at 500 mm/min speed, and the mean values were analyzed using two-way ANOVA and Tukey HSD test (P<0.05). Results: The mean value of tensile strength of RTV (2.96 ± 0.41), 3%SiO2 RTV (3.26 ±0.33), HTV (3.30 ±0.36),3% SiO2 HTV (4.07 ±0.85) MPa which was statistically significant for control, neutral soap and 4% chlorhexidine at 252,504 ,1008 hours of aging. (P <0.05). The percentage of elongation of RTV (545 ±29.2),3%SiO2 RTV (617 ±30.5), HTV (735 ±48.7),3% SiO2 HTV (801 ±55.7) which was statically significant for control, neutral soap, 4% chlorhexidine and Ozone water for 252, 504 ,1008 hours of aging. (P <0.05). Conclusion: The HTV silicone showed more tensile strength and rupture elongation compared to HTV, RTV and RTV silicones reinforced with 3% SiO2 nanoparticles. Ozone water disinfection had least effect on tensile strength and rupture elongation of maxillo-facial silicone compared to other disinfectant.