Nano-sized Plastic Research Articles

Microplastics and Their Role in the Maintenance and Spread of Antibiotic Resistance Genes in Marine Ecosystems

One of the most discussed issues in environmental science and society is plastic pollution of natural ecosystems and its impact on living organisms. This is evidenced by the flow of numerous publications that have appeared in recent years. The results of the studies confirm that the pollution of aquatic ecosystems with plastic waste is considered one of the most severe global environmental problems. The wide distribution of photooxidation and biological degradation products of micro- and nano-sized plastic particles (MP and NP) in the World Ocean is of particular concern, which make the main contribution to biological effects. Catastrophic environmental consequences of marine ecosystems contamination with microplastics are associated not only with economic damage, but also with human health and the biosecurity of mariculture. Human-made plastisphere is a new ecosystem that plays an increasingly significant role in the life of marine microorganisms. Biofilms formed on the rough and hydrophobic surfaces of the plastisphere consist of various taxa of microorganisms and differ in composition from the surrounding marine communities. In addition, they act as depositories and carriers of intra- and extracellular antibiotic resistance genes (ARGs). The purpose of the review is to summarize current information on environmental pollution by microplastics in the context of its role in the conservation and spread of pathogenic bacteria and antibiotic resistance genes in natural environments with an emphasis on marine ecosystems.

Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Daniorerio)

Plastics pose a health hazard to living beings and the environment. Plastic degradation produces nano-sized plastic particles (NPs) that end up in terrestrial and aquatic ecosystems, including oceans, rivers, and lakes. Their presence in air, drinking water, sediments, food, and personal care products leads to a variety of exposure routes for living beings, including humans. The toxicity mechanisms of these nanomaterials (NMs) in living organisms and ecosystems are currently unknown, making it a priority to understand their effects at the molecular and cellular levels. The zebrafish (Zf) (Danio rerio) is a model organism which has a high homology with humans and has been widely used to assess the hazard of different xenobiotics. In this study, the expression changes of different genes in 120 hpf Zf embryos (Zfe) after exposure to polystyrene (PS) NPs (30 nm) at concentrations of 0.1, 0.5 and 3 ppm were investigated. The results showed that the gene encoding heat shock protein (hsp70) was down-regulated in a dose-dependent manner. The genes encoding superoxide dismutase (SOD 1 and SOD 2), apoptotic genes (cas 1 and cas 8) and interleukin 1-β (il1β) were activated at the concentration of 3 ppm PS NP, while the anti-apoptotic gene Bcl2α was inhibited at 0.5 and 3 ppm. In addition, the neurotransmitter-related gene Acetyl-Cholinesterase (ache) was significantly inhibited and the DNA repair genes (gadd45α and rad51) were also down-regulated. In contrast, the mitochondrial metabolism-related gene cox1 did not alter its expression in any of the treatments. Most of the changes in gene expression occurred at the highest concentration of NPs. Overall, the results indicated that NPs generated cellular stress that caused certain alterations in normal gene expression (oxidative stress, apoptotic and inflammatory processes, neurotoxicity and anti-apoptotic proteins), but did not cause any mortality after 120 hpf exposure at the three concentrations assayed. These results highlight the need for further studies investigating the effects, at the molecular level, of these materials in humans and other living organisms.

Response of soybean (Glycine max L.) seedlings to polystyrene nanoplastics: Physiological, biochemical, and molecular perspectives

Micro and nanoplastics are new generation contaminants of global concern. It is important to evaluate the effects on edible products due to the presence of micro- and nano-sized plastics in the treated wastewater. A hydroponic experiment was carried out to explore the effect of polsytrene nanoplastics (PS-NPs; 20 nm) at different concentrations (0, 12.5, 25, and 50 mg L−1) on Glycine max L. (soybean) seedlings for 7-days. In the current study, firstly the uptake of PS-NPs by Glycine max L. (soybean) roots were confirmed by laser confocal scanning microscope. Exposure to PS-NPs, negatively affected growth parameters and increased Fe, Zn and Mn contents in roots and leaves of soybean seedlings. PS-NPs treatments caused oxidative stress in soybean seedlings. The hydrogen peroxide and malondialdehyde contents, showed similar increase pattern in seedlings exposed to PS-NPs. Response to PS-NPs, the level of antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase) and proline content were generally enhanced in roots and leaves of soybean. The expression level of stress-related genes examined in the study included CSD5, FSD3, APX1, and POD up-regulated in PS-NPs treated-soybean seedlings in a tissue specific manner. The results of the present study showed the adverse effects of PS-NPs on soybean seedlings, which may have important implications for the risk assessment of NPs on crop production and environmental safety.

Cytotoxicity and Genotoxicity of Polystyrene Micro- and Nanoplastics with Different Size and Surface Modification in A549 Cells.

BackgroundMicro- and nano-sized plastics (MPs and NPs) have become an environmental issue of global concern due to their small size, strong bio-permeability and high specific surface area. However, few studies have assessed the effect of polystyrene MPs and NPs on human lung cells. In this research, we evaluated the cytotoxicity and genotoxicity of polystyrene (PS) MPs and NPs with different sizes (2 μm and 80 nm) and surface modification (carboxy and amino functionalized polystyrene, pristine polystyrene) in A549 cells.MethodsThe zeta potential and hydrodynamic particle size of five types of PS plastic solutions were measured by dynamic light scattering, and their morphology and degree of aggregation were observed by scanning electron microscopy. After incubation of the PS plastics with A549 cells, the uptake and toxicity of the cells were assessed by fluorescence microscopy, laser scanning confocal microscopy, flow cytometry, MTT, micronucleus formation assay, and reactive oxygen species.ResultsThe cytotoxicity and genotoxicity of A549 cells caused by nano-level PS is more serious than that of micro-level. Compared with unmodified PS-NPs, more surface-functionalized PS-NPs were found inside the cells, especially the accumulation of PS-NH2. Cell viability and the induction of micronuclei (MN) are appreciably impacted in a dose-dependent way. Compared with pristine PS-NPs, functionalized PS-NPs showed stronger cell viability inhibitory ability, and induced more MN scores.ConclusionThis study shows that the intrinsic size properties and surface modification of PS plastics, the interaction between PS plastics and the receiving medium, intracellular accumulation are critical factors for evaluating the toxicological influences of PS plastics on humans.

Exposure of gametes to aged nano-sized plastic particles during fertilization can influence early larval development in the European whitefish (Coregonus lavaretus)

Plastic pollution has been a growing environmental concern for decades, increasingly affecting both marine and freshwater ecosystems worldwide. Nano-sized plastic particles (NPs) potentially have various toxicological impacts on aquatic organisms and the ecosystem; however, less is known about their possible adverse effects on the reproductive biology and offspring traits of fishes. The present study investigated whether an acute exposure of gametes to aged NPs during fertilization affects offspring early mortality, hatching time, body size at hatching or swimming performance of larvae in a common freshwater fish, the European whitefish (Coregonus lavaretus). Using a replicated full-factorial breeding design, we fertilized the eggs of seven females with the milt of seven males both under exposure medium containing aged 270 nm polystyrene NPs and under control medium. In comparison with the control group, exposure of gametes to NPs increased larval body length slightly but significantly, whereas the embryo mortality, hatching time, and larval swimming performance were not affected. Maternal identity affected significantly all the studied offspring traits while paternal identity only affected the offspring length. Our results suggest that the studied acute exposure of gametes to aged NPs might have interfered normal embryonic development by affecting larval size, but this did not seemingly compromise offspring performance.

The impacts of nanoplastic toxicity on the accumulation, hormonal regulation and tolerance mechanisms in a potential hyperaccumulator - Lemna minor L.

Plastic pollution, which is currently one of the most striking problems of our time, raises concerns about the dispersal of micro and nano-sized plastic particles in ecosystems and their toxic effects on living organisms. This study was designed to reveal the toxic effects of polystyrene nanoplastic (PS NP) exposure on the freshwater macrophyte Lemna minor. In addition, elucidating the interaction of this aquatic plant, which is used extensively in the phytoremediation of water contaminants and wastewater treatment facilities, with nanoplastics will guide the development of remediation techniques. For this purpose, we examined nanoplastic accumulation, oxidative stress markers, photosynthetic efficiency, antioxidant system activity and phytohormonal changes in L. minor leaves subjected to PS NP stress (P-1, 100 mg L-1; P-2, 200 mg L-1 PS NP). Our results showed no evidence of PS NP-induced oxidative damage in P-1 group plants, although PS NP accumulation reached 56 µg g-1 in the leaves. Also, no significant changes in chlorophyll a fluorescence parameters were observed in this group, indicating unaffected photosynthetic efficiency. PS NP exposure triggered the antioxidant system in L. minor plants and resulted in a 3- and 4.6-fold increase in superoxide dismutase (SOD) activity in the P-1 and P-2 groups. On the other hand, high-dose PS NP treatment resulted in insufficient antioxidant activity in the P-2 group and increased hydrogen peroxide (H2O2) and lipid peroxidation (TBARS contents) by 25 % and 17 % compared to the control plants. Furthermore, PS NP exposure triggered abscisic acid biosynthesis (two-fold in the P-1 and three-fold in the P-2 group), which is also involved in regulating the stress response. In conclusion, L. minor plants tolerated NP accumulation without growth suppression, oxidative stress damage and limitations in photosynthetic capacity and have the potential to be used in remediation studies of NP-contaminated waters.

Oxidative stress–mediated synergistic deleterious effects of nano- and microplastics in the hypoxia-conditioned marine rotifer Brachionus plicatilis

While pollution due to nano- and micro-sized plastics (NMPs) and hypoxic conditions both occur in coastal areas, the deleterious potential of co-exposure to hypoxia and NMPs (hypoxia and micro-sized plastics, HMPs; hypoxia and nano-sized plastics, HNPs) is largely unclear. Here, we provide evidence for multigenerational effects of HMP and HNP in the marine rotifer Brachionus plicatilis by investigating changes in its life traits, antioxidant system, and hypoxia-inducible factor (HIF) pathway using an orthogonal experimental design, with nanoscale and microscale particles measuring 0.05 μm and 6.0 μm in diameter, respectively, and hypoxic conditions of 0.5 mg/L for six generations. Combined exposure to NMPs and hypoxia caused a significant decrease in fecundity and overproduction of reactive oxygen species (ROS). The HIF pathway and circadian clock genes were also significantly upregulated in response to HMP and HNP exposure. In particular, synergistic deleterious effects of HNP were evident, suggesting that size-dependent toxicity can be a major driver of the effects of hypoxia and NMP co-exposure. After several generations of exposure, ROS levels returned to basal levels and transcriptomic resilience was observed, although rotifer reproduction remained suppressed. These findings help eluciating the underlying molecular mechanisms involved in responses to plastic pollution in hypoxic conditions.

Optimization of coagulation and sedimentation conditions by turbidity measurement for nano- and microplastic removal

Plastic pollution has emerged as a potential threat to drinking water quality. Coagulation and sedimentation processes are suggested to be an effective way of removing small plastic particles from water. However, a conventional jar test consumes a large volume of plastic specimens, producing secondary wastes and hindering a quick optimization of the treatment processes for nano/microplastic removal. This study investigates the monitoring of nano- and microplastic behavior (0.1, 1, and 10 μm-sized polystyrene) in coagulation and sedimentation processes by a simple turbidity measurement. The coagulation and the subsequent floc sedimentation of the plastic particles could be observed in a turbidity vial with a small volume (∼15 mL), allowing a fast screening of coagulant type, dose, sedimentation time, and water environment. In particular, the physicochemical properties of coagulants could be screened in the turbidity monitoring, where the hydrophobic interaction is identified to be important for the coagulation of nano-sized plastic particles. The optimal coagulation/sedimentation conditions from the turbidity monitoring could be applied to the jar test, achieving the high removal efficiencies of nano/microplastic particles. The plastic removal after the coagulation/sedimentation process could be estimated based on linearity between the plastic concentration and the turbidity. The turbidity-driven removal efficiency well corresponds to that derived from the mass-based calculation of the jar test when with a complete floc settling. Our findings suggest that the turbidity measurement can provide a rational optimization of the water treatment processes for the effective removal of nano- and microplastics.

Combined effects of micro-/nano-plastics and oxytetracycline on the intestinal histopathology and microbiome in zebrafish (Danio rerio)

Accumulated evidence has demonstrated that microplastics and oxytetracycline (OTC) affect organisms, but few studies have investigated their combined effects on aquatic organisms. In this study, adult zebrafish (Danio rerio) were exposed to single and binary-combined contamination of micro-, nano-sized polystyrene plastics and OTC for 30 days, and the intestinal histopathology, gut microbiota and antibiotic resistance genes (ARGs) of zebrafish were measured. The results showed that the intestinal epithelial damage increase with the decrease of plastic sizes. Nano-sized plastics, OTC and their combined exposure caused intestinal epithelial damage, and co-exposure with micro-sized plastics reduced the intestinal damage caused by single OTC exposure. The gut microbial communities were affected by the combined exposure to microplastics and OTC. Compared with the blank control, the relative abundance of Fusobacteria increased 12.7 % and 21.1 % in OTC combined with 45–85 μm micro-plastics (MOTC) and 40–54 nm nano-plastics (NOTC), respectively, and that of Bacteroidetes increased 26.2 % and 18.6 % in the MOTC and NOTC treatments, respectively. The effects of MOTC and NOTC on the biodiversity of the zebrafish gut microbiome were different; MOTC increased the biodiversity by 11.3 % compared with the blank control, whereas NOTC decreased the biodiversity by 8.8 % compared with the blank control. Furthermore, the abundance of ARGs in 40–54 nm nano-plastics, MOTC and NOTC treatments was increased 96.9 %, 96.6 % and 68.8 % compared with the control group, respectively. Additionally, significant differences were observed in ARGs characteristics between the micro- and nano-plastics treated groups whether combined with OTC or not. These results are essential to further understand the combined ecotoxicological effects of micro- or nano-plastics and antibiotics on aquatic organisms.

Liberation of plastic nanoparticles and organic compounds from three common plastics in water during weathering under UV radiation-free conditions

A 620-day batch experiment was conducted to examine the generation of nano-sized plastic particles and migration of organic compounds derived from plastic additives and impurities during the weathering of three common plastic types in water with and without reactive oxygen species. The results show that the amount of nanoplastics plus organic compounds liberated from the tested plastic films, as indicated by total organic carbon, was in the following decreasing order: PET >PP > ABS. Hydroxyl radical generated from Fenton-like reaction significantly enhanced the generation of nanoplastics and release of organic compounds from the weathered plastic films via oxidative degradation. Over 30 organic compounds including potentially toxic organic pollutants originated from plastic additives and impurities were detected. There was a marked difference in the plastic nanoparticle size distribution between the deionized water and the water containing reactive oxygen species. The strong oxidizing capacity of hydroxyl radical resulted in rapider disintegration of the coarser nanoparticles (>500 nm) into the finer nanoparticles (<500 nm) and allowed complete decomposition of the nanoplastics with a size <50 nm or even <100 nm. Elevated level of Ca was detected on the surfaces of the ABS and PP nanoparticles. PP- and PET-derived nanoplastics contained heavy metal(loid)s while no heavy metal(loid)s was detected for the ABS nanoparticles. PET nanoparticles had a stronger capacity to bind S- and N-containing organic ligands compared to the other two plastic nanoparticles. The nanoplastics generated from the weathering were irregular in shape, which means that they had larger specific area compared to spherical engineered nanoplastics.

The combined effects of microplastics and the heavy metal cadmium on the marine periphytic ciliate Euplotes vannus

Microplastics could be grazed by marine organisms and possibly transferred to higher trophic levels along the microbial loop. Due to their size and capacity to concentrate heavy metals that trigger joint toxic effects, microplastics (MPs) have already become a severe threat to marine organisms. The detrimental effects of MPs on large marine organisms have been studied, but the combined toxicity of MPs and cadmium (Cd) on protozoan ciliates remains unclear. In the present study, we selected different diameters and concentrations of polystyrene microspheres (PS-MPs) and Cd2+ as model MPs and heavy metals to evaluate their single and combined effects on the periphytic marine ciliate Euplotes vannus in relation to carbon biomass and oxidative stress. The MPs were indeed ingested by Euplotes vannus and significantly reduced the abundance and carbon biomass of ciliate populations. Combined exposure to MPs and Cd2+ not only increased the bioaccumulation of Cd2+ in ciliates but also exacerbated the decrease in ciliate biomass by increasing oxidative stress and membrane damage. In comparison, the effects of nano-sized plastics (0.22 μm) were more harmful than those of micro-sized plastics (1.07 μm, 2.14 μm and 5.00 μm). A smaller size represents a higher potential for penetrating biological members and a stronger adsorption capacity for cadmium. These results provide new insight into the combined toxicity of microplastics and heavy metals on ciliated protozoa and lay a foundation for higher trophic levels and ecosystems.

Microbial and physicochemical responses of anaerobic hydrogen-producing granular sludge to polyethylene micro(nano)plastics

Micro(nano)plastics is an emerging contaminant in wastewater that has showed significant impacts on various biological treatment processes. Nevertheless, the underlying effects of micro(nano)plastics with different concentrations and sizes on the anaerobic hydrogen-producing granular sludge (HPG) were still unclear. This work firstly attempted to illustrate the microbial and physicochemical responses of HPG to a shock load of polyethylene microplastics (PE-MPs) with varied concentrations and sizes. The results revealed that the PE-MPs inhibitory effect on hydrogen production by HPG was both concentration- and size-dependent. Specifically, the increase of PE-MPs concentration and the decline of PE-MPs size to nano-sized plastics (NPs) significantly decreased the hydrogen yield, downgraded to 79.9 ± 2.6% and 63.0 ± 3.9% (p = 0.001, and 0.0002) of control, respectively, at higher MPs concentration and the smaller MPs size (i.e., NPs). The higher PE-MPs concentration and PE-NPs also suppressed extracellular polymeric substances (EPS) generation more severely. The critical bio-processes involved in hydrogen production were disturbed by PE-MPs, with the extent of negative impacts depending on the dosage and size of PE-MPs. These adverse impacts further manifested as granule disintegration and loss of cellular activity. Mechanism analysis highlighted the roles of oxidative stress, leachate released from PE-MPs, interaction between PE-NPs and granules inducing physical crushing of HPG that led to possible direct contact between cells and toxic substances.

An early comparison of nano to microplastic mass in a remote catchment's atmospheric deposition

The existence of nano sized plastic (NP) has been discussed heavily in recent years, however physical proof from environmental samples and direct comparisons to characterized microplastics is limited. Here we compare microplastic (MP) particles and counts (>10 µm) to NP particle (<0.45 µm) mass concentrations from deposition at a remote field site in the French Pyrenees (elevation 1425 m a.g.l.). Using Thermal Desorption – Proton Transfer Reaction – Mass Spectrometry (TD-PTR-MS) analysis, the data shows that NP is present in atmospheric deposition in quantities up to 2.0 × 105 nanograms m−2 day−1 (1.1 × 105 nanograms m−2 day−1 standard deviation), comparable to that of the >10µm microplastic (up to 1.1 × 105 nanograms m−2 day−1, 2.7 × 104 nanograms m−2 day−1 standard deviation). This comparison indicates the quantity of NP and MP may be similar in this atmospheric deposition, however the estimated particle count for NP is understandably multiple orders of magnitude greater compared to MP. Backward trajectory modelling was used to consider the transport of these MP and NP particles. This highlighted the extended spatial influence of NP and its propensity to remain elevated over a 7-day period.

Nano-sized polystyrene plastic particles affect many cancer-related biological processes even in the next generations; zebrafish modeling

With the ever-increasing plastic pollution, the nano-sized plastic particles that are constantly released from the main materials have a greater potential threat. Studies continue on how to eliminate plastic waste, which has become a global problem, from nature. We are aware that complete elimination is not easy at all, but it is not known clearly that even if it is successful, its effects on organisms will also disappear completely. In this study, zebrafish injected with 20 nm-sized polystyrene particles (PS) only during the embryonic period were grown in an environment without plastic exposure. The effects of PS on their offspring embryo/larvae were examined at morphological, molecular and metabolomic levels. Results showed that parental PNP exposure caused significant malformations, decreased survival rate, increased heart rate and blood flow rate, as well as decreased eye size, height and locomotor activity, which were attributed to growth retardation in the offspring. According to the results of whole-mount immunofluorescence larval staining, cell death and reactive oxygen species were significantly increased, while lipid accumulation was decreased in new generation larvae from zebrafish injected with PNP. In order to elucidate the mechanisms underlying these morphological, physiological and molecular damages, the metabolome analyses were performed by evaluating the Q-TOF MS/MS spectra with chemometric analyses in the offspring larvae. According to the metabolomics results, 28 annotated metabolomes suggested by the OPLS-DA analysis that may vary significantly through a variable in projection scores were detected. In addition, it was detected that the significantly increased histopathological findings and immunopositivity of JNK, H2A.X, PI3 and NOP10 in new generation larvae. In conclusion, it has been shown that exposure to PS, even only during the embryonic period, may affect many cancer-related biological processes in the next generation.

Single and combined effects of antibiotics and nanoplastics from surgical masks and plastic bottles on pathogens

Over the last decade, pollution of plastics and antibiotics has increased in its threat to the environment and human health. However, very limited information is available concerning impact of co-presence of plastics and antibiotics on environment and human health. Moreover, the potential ingestion and inhalation of nano(micro)plastics due to the disposable materials has dramatically increased. With the outbreak and spread of the COVID-19 in the world, disposable surgical masks and plastic bottles have been widely used by the public, and their rapid use and improper dispensing can cause to increase plastic pollution risk on human. However, impacts of co-presence of nano(micro)plastics and antibiotics on pathogens have yet been demonstrated. Therefore, this study aims to investigate the impact the individual and combined influences of nano-sized plastics (surgical mask and plastic bottles) and antibiotics (amoxicillin and spiramycin) towards the main susceptible bacterium (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa) by microbial activity, biofilm formation and their biochemical characteristics. The results showed that antimicrobial efficiencies of the tested antibiotics were reduced (approximately 10–98%) with the plastics. Moreover, the biochemical pathways of the microbial activity changed by the plastics entrance. Polymer structure and sorption play the role on the reduction in the inhibition of pathogens. In the meantime, the biofilm formation changed and characteristic of the extracellular polymeric substance with the co-presence of plastics and antibiotics mostly depended on the polymer structure, exposure time and sorption.

Generation of micro(nano)plastics and migration of plastic additives from Poly(vinyl chloride) in water under radiation-free ambient conditions

A batch experiment was conducted to observe the liberation of micro- and nano-sized plastic particles and plastic additive-originated organic compounds from poly(vinyl chloride) under radiation-free ambient conditions. The weathering of PVC films in deionized water resulted in isolated pockets of surface erosion. Additional ●OH from Fenton reaction enhanced PVC degradation and caused cavity erosion. The detachment of plastic fragments from the PVC film surfaces was driven by autocatalyzed oxidative degradation. Over 90% of micro-sized plastic particles were <60 μm in length. The detached plastic fragments underwent intensified weathering, which involved strong dehydrochlorination and oxidative degradation. Further fragmentation of micro-sized particles into nano-sized particles was driven by oxidative degradation with complete dehydrochlorination being achieved following formation of nanoplastics. 20 organic compounds released from the PVC films into the solutions were identified. And some of them can be clearly linked to common plastic additives. In the presence of additional ●OH, the coarser nanoplastic particles (>500 nm) tended to be rapidly disintegrated into finer plastic particles (<500 nm), while the finest fraction of nanoplastics (<100 nm) could be completely decomposed and disappeared from the filtrates. The micro(nano)plastics generated from the PVC weathering were highly irregular in shape.

Index models for ecological and health risks assessment of environmental micro-and nano-sized plastics

&lt;abstract&gt; &lt;p&gt;The definition of environmental indexes is one of the most widely used methods and methodologies for the study of exposure to polluting agents, and it is a highly helpful instrument for describing the quality of the environment in a simple and straightforward manner. In this study, index models were presented and described that can be used in evaluating the contamination, pollution and health risks of environmental micro (MPs) and nanoplastics (NPs) to ecosystems and humans. Index models such as plastic contamination factors (pCf) and pollution load index (pPLI), plastic- bioconcentration or accumulation factors (pBCf or pBAf), plastic-biota-sediment accumulation factor (pBSAf), biota accumulation load index (BALI), polymer risks indices (pRi), polymer ecological risks index (pERI) while plastic estimated daily intake (pEDI) and plastic carcinogenic risks (pCR) were described for oral, dermal and inhalation pathways. All index modeled were further described based on polymer types of MPs/NPs. The final value is represented by a quantity that measures a weighted combination of sub-indices and defined by an appropriate mathematical function. The central concept is to present an indicator that can describe, in a clear and concise manner, the level of MPs/NPs in the environment, thereby indicating where it would be necessary to intervene and where it would not in order to improve overall environmental conditions.&lt;/p&gt; &lt;/abstract&gt;

Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance.

Recently, various waste microplastics sensors have been introduced in response to environmental and biological hazards posed by waste microplastics. In particular, the detrimental effects of nano-sized plastics or nanoplastics have been reported to be severe. Moreover, there have been many difficulties for sensing microplastics due to the limited methodologies for selectively recognizing nanoplastics. In this study, a customized gold nanoparticles (Au NPs) based localized surface plasmon resonance (LSPR) system having bio-mimicked peptide probes toward the nanoplastics was demonstrated. The specific determination through the oligo-peptide recognition was accomplished by chemical conjugation both on the LSPR chip’s 40~50 nm Au NPs and sandwiched 5 nm Au NPs, respectively. The peptide probe could selectively bind to polystyrene (PS) nanoplastics in the forms of fragmented debris by cryo-grinding. A simple UV-Vis spectrophotometer was used to identify the LSPR sensing by primarily measuring the absorbance change and shift of absorption peak. The sandwich-binding could increase the LSPR detection sensitivity up to 60% due to consecutive plasmonic effects. In addition, microwave-boiled DI water inside of a styrofoam container was tested for putative PS nanoplastics resource as a real accessible sample. The LSPR system could be a novel protocol overcoming the limitations from conventional nanoplastic detection.

Microplastic pollution in mountain terrains and foothills: A review on source, extraction, and distribution of microplastics in remote areas

Microplastic (MP) pollution is a critical environmental concern that exists within different mountain ecosystem compartments. This review paper highlights the source, sampling, distribution, and behavior of MPs in mountain terrains and foothills. Atmospheric transport and tourism are major sources of MP pollution in mountain ecosystems. Snow samples provide the maximum concentration of MPs compared to that of stream or ice core samples. Precipitation events considerably influence MP deposition and fallout in mountains and glaciers. PE, PP, PS, polyester, and PVC are common plastic polymers with diverse shapes, such as fibers, fragments, films, and pellets. Ecological concerns and stress due to MP accumulated in natural ecosystems have also been discussed, with considerable focus on MP transport and distribution dynamics at higher altitudes as prospects for future research. A remarkable knowledge gap was observed regarding the MP pathways in the mountainous ecosystems and the assessment of microplastic-associated additives, such as heavy metals and other toxic chemicals, including the evidence of nano-sized plastics. Furthermore, studies on the ecological and biological risks posed by MPs on remote mountains is severely limited with respect to global climate change, biodiversity loss, and influence on ecosystem services.

Polystyrene Nanoplastic Behavior and Toxicity on Crustacean Daphnia magna: Media Composition, Size, and Surface Charge Effects

Concerns about the possible ecotoxicological implications of nano-sized plastic materials in the freshwater environment are growing with the increasing use of plastic materials. The present study focuses on the behavior and effects of amidine-functionalized polystyrene (NPLs) of 20, 40, 60, and 100-nm-size in freshwaters and different synthetic media. Daphnia magna was exposed to increasing concentrations from 0.5 to 30 mg/L (and from 0.5 to 100 mg/L for 100-nm-sized NPLs). The results revealed no significant aggregation in ultra-pure water, culture media, and synthetic water. In the presence of natural organic matter, NPLs of 20 and 40 nm displayed better stability in both freshwater and synthetic media, whereas a significant aggregation of 60 and 100 nm PS NPLs was found. All the studied PS NPLs with size between 20 and 100 nm exhibited acute toxicity to D. magna. The observed 48-h immobilization strongly depended on the primary size of PS NPLs, with 20 and 40-nm-size PS NPLs inducing a stronger effect in both freshwaters and synthetic media. Water quality variables such as pH, cation and anion composition, and DOC were of secondary importance. The results of the present study confirmed the toxicity of NPLs of different sizes to crustaceans in natural freshwater and synthetic media and demonstrated the importance of the primary size of NPLs in the behavior and effects of NPLs.