Nano Hydroxyapatite Research Articles

Fabrication of customized Ti6AI4V heterogeneous scaffolds with selective laser melting: Optimization of the architecture for orthopedic implant applications

Orthopedic implants with heterogeneous porous structures were known as ideal bone osteointegration. This research introduced the selective laser melting (SLM), finite element analysis (FEA), and a hydrothermal process (HT) for manufacturing a three-level heterogeneous porous structure. The macroporous structure was designed via CAD and micropores were tuned via laser power regulation. A nano-size layer of hydroxyapatite crystals was coated by an HT process. The mechanical properties were reinforced via a core-shell structure with core reinforcement. The existence of micropores and nano-hydroxyapatite coating enhanced the in vitro proliferation of preosteoblasts and osteogenic cellular behaviors of rBMSCs. Thus, the three-level heterogeneous porous titanium implants could inspire researchers with potential clue of cyto-implant interaction mechanism, therefore building ideal orthopedic implants with accelerated osteointegration. Statement of significancePorous structures of titanium implants play an important role in bone tissue regeneration; The geometrical environment influence cell behaviour and bone tissue ingrowth in all macro-/micro-/nanoscale. In this study, a novel method to fabricate heterogeneous scaffolds and its macro-/micro-/nanoscopic structures were studied. A CAD model was used to obtain the macroscopic structure and the insufficient laser power was introduced for porous microstructure. Therefore, a layer of nano hydroxyapatite was coated via hydrothermal process. Cytoproliferation and cytodifferentiation results indicated that a integrity of regular/irregular, macro-/micro-/nanoscale porous structure had advance in recruiting stem cells and promoting differentiation. This research is beneficial to the development of bone implants with better bone regeneration ability.

Novel Inorganic Nanomaterial-Based Therapy for Bone Tissue Regeneration.

Extensive bone defect repair remains a clinical challenge, since ideal implantable scaffolds require the integration of excellent biocompatibility, sufficient mechanical strength and high biological activity to support bone regeneration. The inorganic nanomaterial-based therapy is of great significance due to their excellent mechanical properties, adjustable biological interface and diversified functions. Calcium–phosphorus compounds, silica and metal-based materials are the most common categories of inorganic nanomaterials for bone defect repairing. Nano hydroxyapatites, similar to natural bone apatite minerals in terms of physiochemical and biological activities, are the most widely studied in the field of biomineralization. Nano silica could realize the bone-like hierarchical structure through biosilica mineralization process, and biomimetic silicifications could stimulate osteoblast activity for bone formation and also inhibit osteoclast differentiation. Novel metallic nanomaterials, including Ti, Mg, Zn and alloys, possess remarkable strength and stress absorption capacity, which could overcome the drawbacks of low mechanical properties of polymer-based materials and the brittleness of bioceramics. Moreover, the biodegradability, antibacterial activity and stem cell inducibility of metal nanomaterials can promote bone regeneration. In this review, the advantages of the novel inorganic nanomaterial-based therapy are summarized, laying the foundation for the development of novel bone regeneration strategies in future.

Preparation of nano-hydroxyapatite/chitosan/tilapia skin peptides hydrogels and its burn wound treatment

There is an urgent need for wound dressings to treat partial-thickness burns. Hydrogels are a promising material that can maintain hydration to promote necrotic tissue removal. Tilapia peptides (TP) and hydroxyapatite (HA) were incorporated into chitosan system to prepare new types of hydrogels. The hydrogels were cross-linking by tannin (TA), which were developed to promote rapid wound healing in a New Zealand rabbit partial-thickness burn model. Nanohydroxyapatite (NHA) was synthesized by coprecipitation method, which made hydrogels have a highly porous structure comprised of interconnected pores, excellent water absorption and low hemolysis. Besides, the hydrogels showed excellent antimicrobial activities against both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), as well as the cytocompatibility on endothelial cells. Moreover, the hydrogels promoted epithelial and dermal regeneration, reduce the expression of TNF-α and IL-6 and promote the skin regeneration by enhancing expression of collagen, STAT3, and VEGF.

Correlation of heating profile with calcination temperature for the extraction of nano hydroxyapatite (Nano-HAp) derived from bone

Bone is one of the major sources of hydroxyapatite (HAp) in the form of calcium phosphate. The increasing demand for HAp has brought much interest in research line to find the best method for its production either synthetically or naturally. Calcination of natural bone is a common heat treatment method for production of HAp. However, the optimum temperature of calcination depends on source and heating profile of the bone. Therefore, this study aimed to determine heating profile and the optimum calcination temperature of Nano-hyroxyapatite (Nano-HAp) extracted from spotted sardinella (Amblygaster sirm) bone. Characterization of Nano-HAp was carried out using thermogravimetric analysis (TGA), chromameter and X-ray diffraction (XRD) spectroscopy. The composition of protein, ash, moisture and lipid content of raw bone (RB) were 1.08%, 50.98%, 10.47% and 3.78%, respectively. Results demonstrated that the optimum temperature for extracted nano-hydroxyapatite (HAp-1) was 700°C based on yield percentage (55%) and colour changes value (∆E=18). Heating profile of raw bone demonstrated the highest reduction of weight loss percentage (320°C to 610°C=36%); which determined the melting point of the bone that acted as a supporting analysis for calcination. XRD confirmed the crystallinity phase of HAp-1 particle similar to the standard peak of HAp, (PDF No: 1-1008) and comparable with the standard HAp. Based on the heating profile and colour changes value, the findings demonstrated that the calcination of HAp from bone of spotted sardinella (Amblygaster sirm) could be carried out at an optimum temperature of 700°C. Overall findings have suggested that the optimum calcination temperature obtainedmay contribute to the development of optimal method for the extraction of HAp via heat treatment process with different fish species.

Improved healing of critical-size femoral defect in osteoporosis rat models using 3D elastin/polycaprolactone/nHA scaffold in combination with mesenchymal stem cells

Osteoporosis is a common bone disease that results in elevated risk of fracture, and delayed bone healing and impaired bone regeneration are implicated by this disease. In this study, Elastin/Polycaprolactone/nHA nanofibrous scaffold in combination with mesenchymal stem cells were used to regenerate bone defects. Cytotoxicity, cytocompatibility and cellular morphology were evaluated in vitro and observations revealed that an appropriate environment for cellular attachment, growth, migration, and proliferation is provided by this scaffold. At 3 months following ovariectomy (OVX), the rats were used as animal models with an induced critical size defect in the femur to evaluate the therapeutic potential of osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) seeded on 3 dimension (3D) scaffolds. In this experimental study, 24 female Wistar rats were equally divided into three groups: Control, scaffold (non-seeded BM-MSC), and scaffold + cell (seeded BM-MSC) groups. 30 days after surgery, the right femur was removed, and underwent a stereological analysis and RNA extraction in order to examine the expression of Bmp-2 and Vegf genes. The results showed a significant increase in stereological parameters and expression of Bmp-2 and Vegf in scaffold and scaffold + cell groups compared to the control rats. The present study suggests that the use of the 3D Elastin/Polycaprolactone (PCL)/Nano hydroxyapatite (nHA) scaffold in combination with MSCs may improve the fracture regeneration and accelerates bone healing at the osteotomy site in rats.

Synthesis and Characterization of Nano Hydroxyapatite from Waste Egg Shells for Orthopedic and Dental Applications

Hydroxyapatite (HAp) is a type of material that is widely used in orthopedic and dentistry as a bio-coating of implants for improvement in its osteointegration with bone tissue. HAp was processed and synthesized by a precipitation technique by utilizing a biological means eggshell. The synthesis technique involved the calcination of eggshell powder to obtain calcium oxide and then its titration at a controlled flow rate with phosphoric acid. Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray diffraction (XRD) investigations were carried out to confirm the formation of HAp. Whereas, the Scanning Electron Microscopy (SEM) demonstrated the shape and structure of the as-synthesized nanohydroxyapatite powder. The obtained results correlate with the literature. It was observed that the HAp formed was highly crystalline and all the peaks corresponded with HAp based on the standard XRD pattern of HAp (JCDPS card no. 09-432). It was clear from the small peaks that some Tri-Calcium Phosphate was also formed along with the hydroxyapatite. Very less crystallinity can be seen in the SEM micrograph of the eggshell. The nHAp formed was subjected to thermal treatment in a controlled environment. The thermal treatment resulted in improved crystallinity and refined grain size. It is noted that higher surface area biogenic HAp with highly interconnected nanoparticles will be useful in bone morphogenesis during bone surgery.

Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells

Hydroxyapatite (HA) is a representative substance that induces bone regeneration. Our research team extracted nanohydroxyapatite (EH) from natural resources, especially equine bones, and developed it as a molecular biological tool. Polyethylenimine (PEI) was used to coat the EH to develop a gene carrier. To verify that PEI is well coated in the EH, we first observed the morphology and dispersity of PEI-coated EH (pEH) by electron microscopy. The pEH particles were well distributed, while only the EH particles were not distributed and aggregated. Then, the existence of nitrogen elements of PEI on the surface of the pEH was confirmed by EDS, calcium concentration measurement and fourier transform infrared spectroscopy (FT-IR). Additionally, the pEH was confirmed to have a more positive charge than the 25 kD PEI by comparing the zeta potentials. As a result of pGL3 transfection, pEH was better able to transport genes to cells than 25 kD PEI. After verification as a gene carrier for pEH, we induced osteogenic differentiation of DPSCs by loading the BMP-2 gene in pEH (BMP-2/pEH) and delivering it to the cells. As a result, it was confirmed that osteogenic differentiation was promoted by showing that the expression of osteopontin (OPN), osteocalcin (OCN), and runt-related transcription factor 2 (RUNX2) was significantly increased in the group treated with BMP-2/pEH.In conclusion, we have not only developed a novel nonviral gene carrier that is better performing and less toxic than 25 kD PEI by modifying natural HA (the agricultural byproduct) but also proved that bone differentiation can be effectively promoted by delivering BMP-2 with pEH to stem cells.

Synergetic Enhancement of Mechanical Properties for Silk Fibers by a Green Feeding Approach with Nano-hydroxyapatite/collagen Composite Additive

ABSTRACT In the current study, a novel intrinsic functionalization approach based on composite feeding additive for silkworms to produce silk fibers (SFs) with enhanced mechanical properties has been successfully developed. Effect of single feeding additive by either nano hydroxyapatite (Nano-HA) or collagen, and composite feeding by both additives on the silkworm larvae growth and cocooning, crystalline structure, molecular conformationas as well as mechanical properties of SFs have been systematically investigated. Experimental results demonstrated that feeding additive strategy could greatly facilitate the growth and cocooning of the silkworms. Surface and cross-section morphology observation suggested the additive has been absorbed into silkworm body and eventually embedded in SFs. Meanwhile, the basic structure of SFs still kept integrity after feeding additive from X-ray diffraction (XRD) analysis. Furthermore, fourier transform infrared (FTIR) spectrum demonstrated that random coil/α-helix and β-sheet content of SFs by feeding additive presented an increase and decrease change than natural SFs. More importantly, mechanical properties of SFs exhibited an obvious enhancement by composite additive than single additive feeding due to the nanoconfined crystallite toughening and polymer physical blending mechanism. Inspired by this approach, the composite additive feeding may open a novel and green strategy to fabricate SFs with enhanced mechanical properties.

The effect of micro and nano hydroxyapatite powder on biocompatibility and surface integrity of Ti6Al4V (ELI) in powder mixed electrical discharge machining

The problem of achieving functional surface is receiving substantial attention with the demand for efficient medical devices in biomedical industry. To satisfy both mechanical and biomedical concerns, researchers try to develop appropriate commercial machining techniques. This study analyzes the micro and nano Hydroxyapatite (HAp) powder size effect on surface topography and biocompatibility of Ti-6Al-4V (ELI) biomaterial in Electrical Discharge Machining (EDM). We compare thoroughly the titanium surfaces that EDM’ed with three different HAp powder concentrations in Deionized Water (DW) (0 g l−1, 20 g l−1 Micro HAp, 20 g l−1 Nano HAp) and three pulse current levels (7 A, 12 A, 22 A). The roughness of surfaces varies between 0.38 μm and 6.61 μm. Using micro HAp powder in dielectric liquid lowered the surface roughness and enhanced the wettability whereas the nano HAp effect could be negligible. The Energy Dispersive x-ray Spectrometer results show the Ca and P ion migration from the HAp mixed dielectric to the workpiece surface during EDM. The x-ray Diffraction results affirmed the existence of HAp compounds and oxide phases in the surface structure. Biocompatibility tests employing MG63 osteoblast-like cells revealed that for 24 h culture period all EDM samples showed higher viability than the control in vitro. According to the statistical analyses ANOVA (Tukey), the MTT viability results showed a significant difference especially for those machined in micro HAp mixed DW dielectrics. On the other hand, for 72 h culture period, samples machined in DW with 7 and 12 A currents are more biocompatible and have a proliferative effect on MG63 cells. In conclusion, however the dependency on pulse current, powder additive, and topography of the surface exist; EDM can be employed as an effectual treatment to enhance the biocompatibility.

Impact of using nano-hydroxyapatite on postoperative hypersensitivity of two bleaching techniques – randomized controlled clinical trial

To evaluate the impact of using Nano-hydroxyapatite on post-bleaching hypersensitivity after using two different bleaching techniques. Twenty-eight patients were enrolled in this study according to inclusion and exclusion criteria. The patients were randomly assigned into four groups (n=7). G1; bleaching was performed using photo-catalyzed bleaching technique (Philips, zoom), followed by application of Amorphous Calcium phosphate based desensitizing agent (ACP, Relief ACP). G2; bleaching was performed using chemo-catalyzed bleaching technique (Philips, Dash), followed by application of ACP. G3: bleaching was performed using zoom, followed by application of nano-hydroxyapatite based desensitizing agent (Nano p), G4: bleaching was performed using Dash, followed by application of Nano-hydroxyapatite. Post bleaching hypersensitivity was assessed using “Visual analogue scale”. Hypersensitivity was evaluated after: bleaching immediately, application of the desensitizing agent immediately, 24 hours, 48 hours and after one week. Cases with severe hypersensitivity were relieved immediately after Nano p application, meanwhile in case of ACP, cases with severe pain were relieved after 24 hours after its application. Statistically significant difference was evident with the photo catalyzed group immediately after Nano p application than before its application. Reduction in hypersensitivity by time was evident in both the chemo and photo catalyzed groups. In addition, it was found that after one week, all pain scores disappeared with all tested groups. Nano hydroxyapatite particles have an immediate (just post application) relief effect of severe pain with both used bleaching systems. However, the post bleaching hypersensitivity diminished completely after 1 week, irrespective of the desensitizing agent used. The application of Nano hydroxyapatite paste after bleaching is a valid method for diminishing severe post bleaching hypersensitivity when applied with either a photo or chemo catalyzed bleaching agents.

Получение наночастиц гидроксиапатита из естественных зубов

Hydroxyapatite is the most important bio ceramic, due to its structure and chemical composition which is similar to bone and teeth. In this study, hydroxyapatite nanoparticles were prepared from natural teeth in two stages. Initially, the nano HA particles was performed by whole tooth and in the second stage, the tooth was divided into dentine and root and HA nanoparticles were prepared from both portions of teeth. At first, freshly extracted human teeth both (whole tooth and dentine and root) were calcined at 850 °C and then, the effects of stirring time, surfactant and different temperatures were investigated. The XRD results confirmed that, the existence of hydroxyapatite phase in all samples. The phosphate bonding groups of PO43- at 1470 cm-1 and 669 cm-1 was confirmed by FTIR results. The FESEM results showed that, hydroxyapatite nano particle with the range of 29–46 nm at stirring time of 36 h with the addition of CTAB as a surfactant at 850 °C was obtained without agglomeration and good dispersion.

Gelatin/Gelatin-modified nano hydroxyapatite composite scaffolds with hollow channel arrays prepared by extrusion molding for bone tissue engineering

Bone tissue engineering scaffold has been successfully applied in the field of bone repair, but the major limitation is the delivery of oxygen and nutrients throughout the bulk of engineered tissue, especially for large scaffolds. Researchers have found that scaffolds with hollow channels can effectively solve this problem. We utilized a novel extrusion approach to prepare scaffolds with hollow channel arrays, which has the advantages of economy, continuous production, and high efficiency. The hollow channel scaffolds were composed of gelatin and gelatin-modified nano hydroxyapatite (nHAP). X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and atomic force microscope (AFM) results indicate that the prepared gelatin-modified nHAP are nanorod-like particles with the length of 30∼50 nm and width of 5∼15 nm. The nano particle is composed of crystalline HAP grains with the size less than 10 nm and amorphous phases. A part of OH− or are replaced by during preparation process, leading to the transformation of HAP into hydroxyl-carbonated apatite (HCA). The prepared hollow channel scaffolds exhibit much better mechanical properties compared with the sponge porous scaffolds. The axial compressive strength of the hollow channel scaffold with porosity of 51.3 ± 5.2% can reach 25 ± 1.4 MPa, which can meet the mechanical strength requirement as an implant completely. Moreover, the hollow channel scaffold exhibited good in vitro degradability and bioactivity. These results highlight the potential of using gelatin/gelatin-modified nHAP composite scaffolds with hollow channels for bone tissue engineering.

The Efficacy of Non-fluoridated Toothpastes on Artificial Enamel Caries in Primary Teeth: An In Vitro Study.

ABSTRACTAims:The aim of this study was to evaluate the remineralizing effect among various non-fluoridated toothpastes on artificial caries in primary teeth.Materials and Methods:Fifty sound primary incisor teeth were embedded in self-curing acrylic resin and immersed in demineralizing solution for 4 days forming artificial caries. All teeth were divided into five groups (10 teeth/group): Group I deionized water (control); Group II 1000 ppm F (Kodomo®); Group III non-fluoridated toothpaste containing calcium glycerophosphate and calcium lactate (Dokbuaku®); Group IV casein phosphopeptides-amorphous calcium phosphate (CPP-ACP) paste (GC Tooth Mousse®), and Group V non-fluoridated toothpaste containing nanohydroxyapatite (NHA) (Apagard®). The specimens were subjected to pH-cycling and immersed in toothpaste slurry twice for 7 days. Baseline, before, and after pH-cycling surface microhardness (SMH) values were measured by Vickers hardness numbers, and the percentage recovery of SMH (%SMHR) was calculated. Data were analyzed by one-way analysis of variance and Tukey’s multiple comparison at 95% confidence intervals.Results:The SMH values of test groups were significantly higher than those of the control group (p = 0.00). The %SMHR was −5.72±7.03% in the control group, Group II was significantly higher than Group III but there were no significant differences among Groups II, IV, and V (p > 0.05).Conclusion:Non-fluoridated toothpastes containing CPP-ACP or NHA for young children had efficacy in remineralizing effect on primary teeth comparable with 1000 ppm fluoridated toothpaste.

Development of strontium-doped nano hydroxyapatite dentifrice and compare its remineralising potential with a topical cream containing casein phosphopeptide- amorphous calcium phosphate - An In Vitro study.

To develop and evaluate the efficacy of synthesised strontium-doped nano hydroxyapatite dentifrice and compare its remineralizing potential with a topical cream containing Casein Phospho Peptide - Amorphous Calcium Phosphate, in remineralizing artificial carious lesion on enamel. Enamel specimens of 4 x 4 x 1 mm were prepared from 90 freshly extracted teeth. Specimens were divided into 3 groups of 30 samples each, based on the type of dentifrice applied that is a control group (Group I) and two experimental groups (Groups II, III). Surface topography and the calcium/phosphorous ratio of all sound specimen were evaluated using Scanning electron microscope and Energy Dispersive X-ray Analysis (SEM-EDAX). The samples in group I and each of the experimental groups were subjected to demineralisation and the calcium/phosphorous ratio of the demineralized specimen were analysed. The samples were then subjected to remineralisation using different agents in each group. Samples in the control group (Group I) were brushed with a conventional dentifrice. In the experimental groups, Group II topical cream with Casein Phosphopeptide and Amorphous Calcium phosphate (CPP-ACP) was used and in Group III laboratory synthesized Strontium-doped nanohydroxyapatite paste (Sr-nHAP), respectively for 28 consecutive days. The samples in the both the control and the two experimental groups were again subjected to SEM-EDAX analysis to analyse the calcium phosphorus ratio following remineralisation cycle. Groupwise comparison of the data was done with one way ANOVA followed by Tukeys Post hoc Test. Both experimental groups (II, III) showed statistically significant remineralisation potential after demineralisation, compared to the control group I. Intergroup comparison showed that the samples in Group III showed the higher remineralisation potential than Group II and was statistically significant. Both CPP- ACP containing tooth cream as well as Sr doped nHAp showed remineralisation potential. Sr doped nanohydroxyapatite showed better remineralisation than CPP ACP and can be considered for enamel repair in incipient carious lesions.

High Performance Nano Hydroxyapatite Coating on Zinc for Biomedical Applications

High Performance Nano Hydroxyapatite Coating on Zinc for Biomedical Applications

Antimicrobial activity of a remineralizing paste containing Strontium doped Nano hydroxyapatite (Sr-nHAp) with Non Collagenous Protein (NCP) analogue Chitosan – An in vitro study

Dental caries is an infectious multifactorial disease. Biomimetic remineralisation of non-infected carious dentine helps in retaining tissues to a maximum degree during vital pulp treatment. However micro gaps and residual bacteria below restorations lead to microleakage, degradation of tooth structure and secondary caries. Remineralizing agent with anti-bacterial efficacy would help contain secondary caries and its sequelae. The aim of the study was to evaluate the antimicrobial activity of remineralizing paste containing Strontium doped Nano hydroxyapatite (Sr-nHAp) with Non Collagenous Protein (NCP) analogue, Chitosan. In this study dentin slabs, 200 µm thick obtained from freshly extracted premolars were demineralised and inoculated with Streptococcus mutans to simulate artificial carious lesion. The specimens were divided in to six groups based on the reminerlising regimen, Group 1–10 wt%Sr-nHAp, Group 2–20 wt%Sr-nHAp, Group 3–10 wt%Sr-nHAp + Chitosan and Group 4–20 wt%Sr-nHAp + Chitosan, Group 5 Positive control – Calcium hydroxide and Group 6, negative control, no treatment. The dentin specimens were homogenised and assessment of bactericidal effects was done by counting the number of viable bacteria after 48 h of incubation. Agar well diffusion test was performed to measure the zone of inhibition. Results were tabulated and analysed statistically using, ANOVA and Post – hoc Bonferroni tests (P = 0.05). All experimental groups except Group 1, exhibited antibacterial properties observed by Zone of Inhibition and reduced colony forming units. Antibacterial efficacy of the remineralizing paste was enhanced by the addition of Chitosan and its antimicrobial efficacy was comparable to calcium hydroxide.

Characteristic of polypropylene nanocomposite material reinforcement with hydroxyapatite for bone replacement

Purpose: Human bone suffered some degeneration due to age and accidents; therefore, there are many interests in the prepared synthetic bone with properties nearer to natural bone. The present study prepared a nanocomposite of polypropylene reinforced with different weight fraction of Nano hydroxyapatite (HAp) to be used as a bone replacement with good biological properties that enhanced the growth of osteoplastic cells and enhance the prevention of clots and coagulates creation. Design/methodology/approach: Nanocomposite from polypropylene reinforced with different weight fraction of Hydroxyapatite (HAp) (1,2 and 3) % prepared by first dispersion Nano hydroxyapatite insolvent and then mixing with a pellet of polypropylene by the twinscrew extrusion process, the current research study the surface properties ( atomic force microscopy (AFM), contact angle test) Moreover, it studied the characteristics of prepared nanocomposite materials (Differential Scanning Calorimetry (DSC), Field Emission-Scanning Electron Microscopy (FE-SEM) and Fourier Transform Infrared (FTIR)). Findings: The AFM results show the surface roughness decreased with increasing content of HAp, which diminished the chance of creation clots and coagulates on it. The contact angle results referred to polypropylene behaviour transformed from hydrophobic to hydrophilic with addition HAp that permission to grow the osteoplastic cell on it, so the healing process is accelerated. Moreover, the FE-SEM images revealed uniform distribution and good bonding between polypropylene and Hydroxyapatite. The thermal properties were measured by the DSC test showed the melting temperature, and the enthalpy of melting (indicated to increase the crystalline structure per cent) are increased with increasing the percentage of Hydroxyapatite. Research limitations/implications: This research studied the characteristics of nanocomposite materials prepared by three steps (dispersion by ultrasonic device, manually mixed and melting and mixing by twin extruder) which can be used as a bone replacement. However, the main limitation was the uniform distribution of nano-hydroxyapatite within the matrix. In a further study, the cytotoxic test can be tested to study the effect of prepared nanocomposite on living cells’ growth. Practical implications: The interest object is how to connect among different properties to prepared bone replacement with good properties and biocompatibility that made able to stimulate the growth and healing process. Originality/value: The nano-hydroxyapatite is a biomaterial that has a composition similar to the natural mineral phase of the bone and does not have any negative effect, which enhanced the growth of osteoplastic cells and decreased the clots and coagulates creation; therefore, nano-hydroxyapatite is used to decrease the surface roughness which decreased the chance of coagulation creation and to enhance the hydrophilic properties.

Clinicoradiographic evaluation of hyaluronan-nano hydroxyapatite composite graft in the management of periodontal infrabony defects.

Background:Hyaluronan is a naturally occurring polysaccharide in the extracellular matrix of the connective tissue. It imparts antibacterial and osteogenic properties to the nano hydroxyapatite bone graft (NHA).Aim:This study aims to evaluate the efficacy of hyaluronan-NHA (H-NHA) composite in the treatment of infrabony defects in chronic periodontitis patients.Materials and Methods:Eight systemically healthy chronic periodontitis patients with 32 graftable sites were included in the study. After flap reflection and debridement, the defect sites in the test quadrant were filled with H-NHA graft and those in the control quadrant were filled with NHA graft alone. Clinical parameters such as probing pocket depth (PPD) and clinical attachment level (CAL) were assessed at baseline and 3, 6, 9, and 12 months; bone probing depth (BPD) and radiographic parameters such as amount and percentage of defect depth reduction (DDR, PDDR) and change in alveolar crest level (ALR) were assessed at baseline and 6 and 12 months.Statistical Analysis Used:Descriptive statistics, one-way analysis of variance, and Student's t-test were used for statistical analysis.Results:At the end of 12 months, H-NHA group showed a greater reduction in PPD (5.06 ± 0.582 mm), BPD (4.22 ± 0.371 mm), and gain in CAL (4.00 ± 0.421 mm) as compared to the NHA group (3.21 ± 0.648 mm, 3.21 ± 0.047 mm, and 2.86 ± 0.127 mm, respectively). In addition, DDR, PDDR, and ALR were better in H-NHA group (1.92 ± 0.647 mm 48.22 ± 31.561 mm, and 1.22 ± 0.808 mm, respectively) as compared to the NHA group (1.14 ± 0.602 mm, 20.14 ± 25.349 mm, and 0.89 ± 0.626 mm, respectively). Statistically significant improvements in all the parameters were seen in the test sites when compared to control sites at 12 months.Conclusion:H-NHA composite graft can be considered a promising periodontal regenerative material.

Aligned Nanofiber-Guided Bone Regeneration Barrier Incorporated with Equine Bone-Derived Hydroxyapatite for Alveolar Bone Regeneration.

Post-surgery failure of dental implants due to alveolar bone loss is currently critical, disturbing the quality of life of senior dental patients. To overcome this problem, bioceramic or bone graft material is loaded into the defect. However, connective tissue invasion instead of osteogenic tissue limits bone tissue regeneration. The guided bone regeneration concept was adapted to solve this problem and still has room for improvements, such as biochemical similarity or oriented structure. In this article, an aligned electrospun-guided bone regeneration barrier with xenograft equine bone-derived nano hydroxyapatite (EBNH-RB) was fabricated by electrospinning EBNH/PCL solution on high-speed rotating drum collector and fiber characterization, viability and differentiation enhancing properties of mesenchymal dental pulp stem cell on the barrier was determined. EBNH-RB showed biochemical and structural similarity to natural bone tissue electron microscopy image analysis and x-ray diffractometer analysis, and had a significantly better effect in promoting osteogenesis based on the increased bioceramic content by promoting cell viability, calcium deposition and osteogenic marker expression, suggesting that they can be successfully applied to regenerate alveolar bone as a guided bone regeneration barrier.

Electrospinned bioactive glass/nano hydroxyapatite reinforced polycaprolactone GTR/GBR membranes in periodontics- A review

Periodontitis, a chronic inflammatory infectious disease triggered by an interaction between microbial biofilm and host response leading to gingival bleeding, connective tissue destruction , alveolar bone loss and finally causing tooth loss. Periodontal regeneration or restoration is the ultimate goal of any periodontal treatment. This can be achieved by GTR/GBR [Guided Tissue Regeneration/Guided Bone Regeneration] membranes along with bone replacement grafts. With the evolution of Tissue engineering and different generation of GTR/GBR nanofibrous membranes, periodontal restoration or regeneration can be easily and rapidly achieved . Nanofibrous GTR/GBR Membranes can be fabricated using natural or synthetic polymers by different techniques such as Phase separation, Self assembly and Electrospinning [E-spinning]. Of these, E-spinning is the most widely studied technique and also seems to exhibit the most promising results for Tissue Engineering[TE] applications . This review addresses the Electrospinning method of GTR/GBR membrane fabrication using Bioactive Glass [BG]/Nano Hydroxyapatite[nHAP] reinforced polycaprolactone polymer and its advantages and disadvantages. Keywords: Electrospinning, Guided Tissue Regeneration, Periodontal Regeneration