Abstract
Post-surgery failure of dental implants due to alveolar bone loss is currently critical, disturbing the quality of life of senior dental patients. To overcome this problem, bioceramic or bone graft material is loaded into the defect. However, connective tissue invasion instead of osteogenic tissue limits bone tissue regeneration. The guided bone regeneration concept was adapted to solve this problem and still has room for improvements, such as biochemical similarity or oriented structure. In this article, an aligned electrospun-guided bone regeneration barrier with xenograft equine bone-derived nano hydroxyapatite (EBNH-RB) was fabricated by electrospinning EBNH/PCL solution on high-speed rotating drum collector and fiber characterization, viability and differentiation enhancing properties of mesenchymal dental pulp stem cell on the barrier was determined. EBNH-RB showed biochemical and structural similarity to natural bone tissue electron microscopy image analysis and x-ray diffractometer analysis, and had a significantly better effect in promoting osteogenesis based on the increased bioceramic content by promoting cell viability, calcium deposition and osteogenic marker expression, suggesting that they can be successfully applied to regenerate alveolar bone as a guided bone regeneration barrier.
Highlights
The human organs and tissues are developed in aligned matter
Almost every equine bone-derived nanohydroxyapatite (EBNH)-RB showed HA with a similar X-ray Diffractometry (XRD) peak at 2θ = 22.5 and 25 except for the PCL-only group. These results show that EBNH maintained HA-like chem ical properties even after being electrospun with PCL (Figure 2c)
The results suggest that The alignment cells by synergistic effects of alignment and results sugg of PCL and EBNH
Summary
The human organs and tissues are developed in aligned matter. Tendon [1] and muscle [2] are such examples, and even bone tissue [3] exists in an aligned manner as well. Aligned structures in tissues serve many purposes, providing physical strength and unique functions. It is well known that providing micro- to nanoscale aligned topographic structures and physical strength, as in vivo, can enhance the activity of the cells grown above. Previous studies reported that the alignment of surface topography can have synergetic effects with the physicochemical properties of surface materials to promote osteogenic differentiation of cells, which can be considered an appropriate strategy to adapt in many tissue engineering examples [5,6,7,8]
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