Musk Fragrances Research Articles

Microplastics ingestion and endocrine disrupting chemicals (EDCs) by breeding seabirds in the east tropical Atlantic: Associations with trophic and foraging proxies (δ15N and δ13C)

In this study we found that endocrine disrupting chemicals (EDCs) were omnipresent in a tropical seabird community comprising diverse ecological guilds and distinct foraging and trophic preferences. Because EDCs tend to bioaccumulate within the food web and microplastics can absorb and release harmful chemical compounds, our findings draw attention to the potential threats to wildlife. Thus, the goal of this study was to investigate the role of plastic ingestion, trophic and foraging patterns (δ15N and δ13C) of five tropical seabird species breeding in sympatry, on the exposure to EDCs, namely Polybrominated diphenyl ethers (PBDEs), methoxylated polybrominated diphenyl ethers (MeO-PBDEs) and personal care products (PCPs, e.g., musk fragrances and UV-filters). Results indicated that microplastics occurrence and EDCs detection frequency varied among species. Microplastics occurrence was higher in species with dual and coastal foraging strategies. Preen oil had higher levels of MeO-PBDEs and PCPs, while serum had higher levels of PBDEs. In brown boobies, the correlation between microplastics and ∑PBDEs levels was significant, suggesting that microplastics ingestion is a key PBDEs route. Trophic position (δ15N) plays a key role in PBDEs accumulation, particularly in Bulwer's petrel, which occupies a high trophic position and had more specialized feeding ecology than the other species. MeO-PBDEs were linked to foraging habitat (δ13C), although the link to foraging locations deserves further investigation. Overall, our findings not only fill key gaps in our understanding of seabirds' exposure to microplastics and EDCs, but also provide an essential baseline for future research and monitoring efforts. These findings have broader implications for the marine wildlife conservation and pollution management in sensitive environments, such as the tropical regions off West Africa.

Behaviour of synthetic musk fragrances in freshwaters: occurrence, relations with environmental parameters, and preliminary risk assessment

The aims of this study were to investigate the presence, possible sources, and potential ecological risks of synthetic musk fragrances in freshwaters and sediments of the main tributaries of a deep subalpine lake in Northern Italy. The total musk concentrations ranged from few ng L−1 up to values > 500 ng L−1, depending on river characteristics: water flow and the presence of wastewater effluents proved to be the main factors affecting fragrance concentrations. The water flow may indeed dilute fragrance input mainly deriving from treated wastewaters. Good correlations (determination coefficients > 0.60) between synthetic fragrances concentrations and parameters related to anthropogenic impacts confirmed this hypothesis: synthetic fragrances were mainly detected in most polluted rivers crossing urbanized areas. Sediment analysis highlighted accumulation of fragrances in this matrix. Concentrations of synthetic fragrances up to 329 ng g−1 organic carbon were measured in sediments of the most contaminated rivers Boesio and Bardello, which also show the highest nutrient content. The preliminary environmental risk assessment revealed that present levels of synthetic musk fragrances do not pose any risk to the studied environmental compartments. However, a probable medium risk level was evidenced during the dry season in the most contaminated rivers Boesio and Bardello. For these reasons, small rivers draining urbanized watersheds and affected by wastewater effluents should be considered synthetic musk contamination hotspots that warrant further research.

Investigation of the adsorption mechanism of two nitro musk odorants on OR1A1: Advanced modeling and thermodynamic study

The experimental dose-olfactory response curves of the two nitro musk odorants, such as musk tibetene and musk ambrette, on the broadly human olfactory receptor OR1A1 have been analyzed using four statistical physics models by means of the grand canonical formalism. For such a purpose, the monolayer model with one energy level and the monolayer model with two energy levels were selected to describe the adsorption mechanism of musk tibetene and musk ambrette, respectively. Indeed, three main physicochemical parameters introduced in the selected models affecting the putative adsorption phenomenon involved in the sense of smell, namely, the olfactory response at saturation, the number of nitro musk odorants per OR1A1 binding site, and the half saturation concentration related to the molar adsorption energy. Actually, the investigation of the number of nitro musk fragrances per binding site showed that musk tibetene and musk ambrette odorants were docked on OR1A1 binding sites by a non-parallel orientation and a multi-docking mechanism since the values of this stereographic parameter were higher than unity. Moreover, the selected adsorption models were used to estimate the broadly tuned human olfactory receptor site size distributions relative to musk tibetene and musk ambrette, which were spread out from 1 to 30 nm with a maximum at 4.10 nm for musk tibetene and at 3.55 nm for musk ambrette. Energetically speaking, the magnitudes of the molar adsorption energies, which were inferior to 5 kJ/mol, indicated that the two nitro musk fragrances were physisorbed on OR1A1 binding sites. Furthermore, the adsorption energy distributions, relative to the two olfactory systems, may be estimated to deeply comprehend human olfactory perception, at a molecular level, via the determination of two approximate olfactory bands through the estimation of two adsorption energy distribution bands, which were spread out from 0 to 8.50 kJ/mol for musk tibetene and from 0 to 8 kJ/mol for musk ambrette. Lastly, macroscopically speaking, three thermodynamic potentials governing the adsorption mechanism were studied. Hence, the disorder during the putative adsorption mechanism was followed via the study of the adsorption entropy. Lastly, the adsorption process introduced in the two olfactory systems was spontaneous and exothermic as it was suggested by the Gibbs free energy and the internal energy.

Particle-associated organic contaminant and cytotoxicity transport in a river during storm events

Storm events lead to mobilization of particles as well as organic contaminants that can pose a risk to river ecosystems. Assessment of total loads of particle-bound and dissolved contaminants in rivers is challenging, especially during rain events since multiple, often unknown contamination sources may be triggered, among them tributaries, combined sewer overflows, direct soil and road runoff, and the riverbed sediment. We investigated whether cytotoxicity (i.e., reduction of the cell viability of human cell lines) could be used as a proxy for the total chemical mixture to assess the transport of organic contaminant mixtures either in particle-associated form or in the aqueous phase. Time-proportional composite samples were collected in the Ammer River (Southwestern Germany) during two storm events in June 2021. All samples were screened for 127 organic contaminants and cytotoxicity in the water and particle phase separately. In total, 56 organic compounds were found, of which 14 and 34 were detected only in the water and particle extracts, respectively, and eight compounds were present in both phases. Thermodynamic equilibrium between the aqueous phase and suspended solids was observed for four compounds namely triphenyl phosphate, octocrylene, galaxolide, and p-chlorocresol. Substances that were not in equilibrium were mainly constituents of plastics and tire rubber, and their high concentrations in the particle phase were probably due to extractions from the particles themselves. Linear correlations between TSS and total compound concentrations were found for 17 PAHs and two musk fragrances, suggesting stable particle loadings and aqueous concentrations over the course of the rain event, most likely due to particle delivery from a particle source with a constant degree of contamination. The total cytotoxicity of water plus particles in the river correlated linearly with TSS during storm events. Inflowing water and particles from all sources appeared to be integrated, such that the total cytotoxicity of all contaminants per mass of particles and volume of water in the river was stable throughout storm events. From this relationship, a threshold of 0.5 g L-1 TSS was derived, above which particle-facilitated transport exceeded dissolved cumulative contaminant transport in the Ammer River, indicating the relevance of particle-facilitated contaminant transport.

Seasonal occurrence and risk assessment of endocrine-disrupting compounds in Tagus estuary biota (NE Atlantic Ocean coast).

Estuaries are continually threatened by anthropogenic pressures, consequently, a large group of contaminants harmful to human health affects the aquatic biota; therefore, it is necessary to monitor their quality. This study deals with the determination of a large group of compounds representing different endocrine-disrupting compounds (EDCs) classes [21 pesticides, 4 polycyclic musk fragrances, 4 UV-filters, 7 bisphenols, 6 polybrominated diphenyl ethers (PBDEs) and 8 of their methoxylated (MeO-BDEs)] in several estuarine species (fish, bivalves, crustaceans, earthworm, and macroalgae) collected seasonally along one year in two distinct areas of Tagus River estuary ("contaminated" vs. "clean" areas). The most abundant compounds found were galaxolide (HHCB) (81% positive samples; 0.04-74ng/g ww), isoamyl 4-methoxycinnamate (IMC) (64%; 1.13-251ng/g ww), alachlor (44%; 0.08-16ng/g ww), and BDE-47 (36%; 0.06-2.26ng/g ww). Polycyclic musks were the most frequent contaminants in fish (seabass, barbus, mullet, and sole) and macroalgae samples, while UV-filters were predominant in bivalves and crustaceans, and bisphenols in earthworms. Seasonal variation was verified for Σpesticides and Σmusks, with significantly higher levels in summer and autumn, whereas ΣUV-filters highest levels were found in spring and summer, and for ΣPBDEs statistically higher levels were registered in cold seasons (autumn and winter). Σbisphenols were significantly lower in spring than in the other seasons. In general, considering all species analysed in both areas, no statistically significant differences (p>0.05) were verified between the two collection areas. Based on the estimated daily intake data, consumption of fish from this estuary is unlikely to be a human health concern, since the levels of contamination were below the toxicological threshold values. Overall, the data obtained in this study will allow regulatory authorities to identify and prioritize contaminants monitoring programs in estuaries, such as the case of bisphenol A, which was found, for the first time, in earthworm and clam species.

Occurrence and risk assessment of endocrine-disrupting compounds in fish muscle: The case study of the Douro River estuary (North East Atlantic Ocean)

This work was focused on evaluating the occurrence of endocrine-disrupting compounds (EDCs) in fish muscles, such as bisphenol analogues, personal care products, including some UV-filters and musk fragrances, and selected pesticides. A total of 238 muscle samples of three fish species (Platichthys flesus, Mugil cephalus, and Dicentrarchus labrax) of an important estuary ending in the North East Atlantic Ocean (Douro River estuary, Portugal), in different seasons of the year, was analysed. M. cephalus was the species with a greater occurrence of contaminants, mainly in fishes collected during the spring. Bisphenol A was the main bisphenol analogue detected with an average content of 23.1 μg/kg wet weight (w.w.). Among chemicals used in personal care products, galaxolide, tonalide, and 2-ethylhexyl salicylate, were the most often found, being present in 15.9% (2.2–538.4 μg/kg w. w.), 4.6% (1.1–57.8 μg/kg w. w.) and 3.4% (3.9–56.1 μg/kg w. w.) of the samples, respectively. About 14% of the samples contained residues of at least one pesticide, being alachlor, aldrin, p,p’-DDT, permethrin, and prochloraz the main chemicals observed, varying from 0.1 μg/kg w. w. (p,p’-DDT) to 37.8 μg/kg w. w. (prochloraz). The daily intake estimates of the most frequent EDCs found in the fish muscles suggested that there are no health concerns, based on the recommended weekly consumption of fish for adults.

Integrated Exposure and Algal Ecotoxicological Assessments of Effluents from Secondary and Advanced-Tertiary Wastewater-Treatment Plants.

The great concern over the environmental impact of wastewaters has led to the designing of advanced treatment processes to upgrade conventional treatment plants and achieve a significant reduction of contaminants in receiving waters. In the present study we combined chemical and ecotoxicological analyses, aiming to evaluate the reduction of toxicity effects associated with the removal of micropollutants and to define the contribution of the detected compounds to the overall toxicity of the mixtures in a series of wastewater effluents collected from a secondary treatment (OUT 2) and from a tertiary activated carbon treatment (OUT 3) plant. The target compounds were selected after a screening procedure among pharmaceuticals, musk fragrances, and trace metals. The classical algal growth inhibition test was conducted on the original effluent samples and on different fractions obtained by solid-phase extraction (SPE) treatment. A good accordance was found between the removal of toxicity (30%-80%) and organic compounds (70%-80%) after the tertiary treatment, suggesting its high efficiency to improve the wastewater quality. The discrepancy between the contribution to the overall toxicity of the nonadsorbable compounds (i.e., inorganic or very polar organic compounds) as experimentally measured by the SPE bioassays (18%-76%) and calculated by the concentration addition approach (>97%) could be mitigated by including the bioavailability correction in metal-toxicity modeling of wastewater mixtures. For the organic compounds, the toxic equivalency method enabled us to quantify the portion of toxicity explained by the detected chemicals in both OUT 2 (82%-104%) and OUT 3 (5%-57%), validating the selection of the target molecules. The applied integrating approach could be implemented by the inclusion of both additional target chemicals and toxicity endpoints. Environ Toxicol Chem 2022;41:2404-2419. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Uptake and translocation of synthetic musk fragrances by pea plant grown in sewage sludge-amended soils

Sewage sludges are rich in organic matter and several essential nutrients for plant growth, making them very appealing for application in agricultural soils. However, they may also contain a wide range of emerging pollutants, which has raised concerns about the potential risks of this practice to crops, the environment, and public health - accumulation in soils and/or plant uptake and translocation of contaminants. Therefore, there is a need to study plant-soil interactions and assess the uptake potential of these contaminants by food crops to better understand these risks. The main aim of this work was to assess the possible drawbacks of sludge application to cropland, by observing the impact on the growth and yield of a model crop (pea plant - Pisum sativum) grown over an 86-day greenhouse experiment and by assessing the uptake potential of synthetic musk fragrances. Different sewage sludge application rates (4–30-ton ha−1) and initial concentrations of contaminants were tested. The application of sludge yielded benefits to the cultivated plants, finding improved crop productivity with an application rate of 30-ton ha−1. At the end of the experiment, soil samples and plants separated into sections were analysed using a QuEChERS extraction methodology followed by gas chromatography-mass spectrometry (GC-MS) quantification. Galaxolide (HHCB) and tonalide (AHTN) underwent uptake by the plant roots, having been detected in concentrations up to 346 ng g−1 on a dry weight basis (dw), but only HHCB was detected in above ground tissues. At the end, a decrease in the levels of synthetic musks in the amended soils (>80% in several instances) was observed. Assuming the worst-case scenario, no risk to human health was observed from the ingestion of peas grown on sewage sludge-amended soils. However, a soil hazard quotient analysis yielded worryingly high quotient values for AHTN in nearly all tested conditions.

Adsorption of pesticides and personal care products on pristine and weathered microplastics in the marine environment. Comparison between bio-based and conventional plastics

The hydrophobicity of persistent organic pollutants (POPs) makes them adsorb on microplastics in the marine environment, affecting their distribution, persistence, or their transfer to the trophic chain. Fragrances and non-polar pesticides can be adsorbed by microplastics in the marine environment because of their physico-chemical characteristics.In this work, the adsorption of two pesticides (α-endosulfan and chlorpyrifos) and 6 musk fragrances (musk xylene, musk ketone, musk moskene, galaxolide, tonalide, and celestolide) on polyamide (PA6) (a petroleum based polymer) and on polyhydroxybutyrate (PHB) (biopolymer) in seawater was studied, considering also the effect of water temperature and plastic weathering.Results show higher adsorption of the selected pollutants for PHB than PA, being PA more affected by the water temperature and the plastic weathering. The highest percentage of adsorption was achieved in most cases at 24 h. In addition, this process was irreversible, as it showed the leaching assays. Besides, this work revealed that plastics mitigate the degradation of α-endosulfan in aquatic media (hydrolysis), showing that plastics can act as inhibitors of degradation of POPs, increasing its persistence in the environment.

The impact of synthetic musk compounds in biofilms from drinking water bacteria

Musk fragrances have been detected in drinking water (DW) at trace concentrations. However, their impact on the microbial quality of DW has been disregarded. This work provides a pioneer evaluation of the effects of two synthetic musks contaminants, tonalide (AHTN) and galaxolide (HHCB), in microbial biofilms formed on two different surfaces, polyvinyl chloride (PVC) and stainless steel AISI 316 (SS316). Three bacterial species isolated from DW (Acinetobacter calcoaceticus, Burkholderia cepacia and Stenotrophomonas maltophilia), were used to develop 7-day-old single and mixed species biofilms. The impact of musks was assessed directly on biofilms but also on the bacteria motility, biofilm formation ability and biofilm susceptibility to chlorination. AHTN musk caused the most remarkable effects by increasing the cellular density and viability of mixed biofilms, and the extracellular polysaccharides content of biofilms on SS316. Most of the alterations caused by the direct exposure of biofilms to musks were observed when SS316 was used as an adhesion surface. In contrast, the ability to form biofilms and their susceptibility to chlorine were more affected for bacteria from HHCB-exposed biofilms on PVC. The overall results demonstrate that the presence of musks at residual concentrations influences DW bacterial dynamics, with the potential to impact the DW quality and safety. The type of plumbing material may further impact the effects of musks.

Complex chemical cocktail, containing insecticides diazinon and permethrin, drives acute toxicity to crustaceans in mountain lakes

Mountain lakes have long been perceived as pristine environments. However, atmospheric deposition of persistent organic pollutants (POPs) have been shown to expose these sensitive ecosystems to chemical pollution. Little is known on how this pollution impacts aquatic ecosystems at high altitudes. We combined passive sampling with liquid and gas chromatography high resolution mass spectrometry (LC- and GC-HRMS) to screen the water of eight lakes in three different regions of the French Pyrenees. In total, we screened for 479 organic chemicals including POPs, polycyclic aromatic hydrocarbons (PAHs), legacy and current-use pesticides, biocides, and musk fragrances. We detected a complex cocktail of 151 individual chemicals and used their toxic unit summation (ΣTU) to assess toxicity for crustaceans and algae. While risks for algae never reached chronic risks, this was always the case for crustaceans. Acute toxic risk thresholds for crustaceans were even exceeded in several of our sites. At sites with acute toxic risk levels (> 0.1 ΣTU) crustaceans were completely absent or showed a low abundance. We conclude that crustaceans were at least partly impacted by the high toxic risks driven by the insecticides diazinon and permethrin. These drugs are widely used to protect livestock from blue tongue disease transmitted by sucking insects, suggesting free roaming livestock as local source. Our results provide important evidence on toxic chemical pollution in relatively remote mountain areas, with important consequences for aquatic mountain ecosystems.

Synthetic Musk Fragrances in Water Systems and Their Impact on Microbial Communities

The presence of emerging contaminants in aquatic systems and their potential effects on ecosystems have sparked the interest of the scientific community with a consequent increase in their report. Moreover, the presence of emerging contaminants in the environment should be assessed through the “One-Health” approach since all the living organisms are exposed to those contaminants at some point and several works already reported their impact on ecological interactions. There are a wide variety of concerning emerging contaminants in water sources, such as pharmaceuticals, personal care products, house-care products, nanomaterials, fire-retardants, and all the vast number of different compounds of indispensable use in routine tasks. Synthetic musks are examples of fragrances used in the formulation of personal and/or house-care products, which may potentially cause significant ecotoxicological concerns. However, there is little-to-no information regarding the effect of synthetic musks on microbial communities. This study reviews the presence of musk fragrances in drinking water and their impact on aquatic microbial communities, with a focus on the role of biofilms in aquatic systems. Moreover, this review highlights the research needed for a better understating of the impact of non-pharmaceutical contaminants in microbial populations and public health.

Multi-analyte gas chromatography-mass spectrometry method to monitor bisphenols, musk fragrances, ultraviolet filters, and pesticide residues in seafood

A gas chromatography-mass spectrometry method for the analysis of thirty-six anthropogenic contaminants in the edible portion of four distinct seafood items is reported. Considering the heterogeneous composition of algae, mussels, and lean/fatty fish muscles, a generic sample preparation based on the QuEChERS procedure combined with dispersive liquid-liquid microextraction (DLLME) with in situ acetylation was successfully applied for quantification of pesticide residues, bisphenols, musk fragrances and UV-filters. Matrix effects were influenced by the type of seafood, with the lowest effects being observed with EMR-lipid and graphitized carbon black sorbents in dispersive solid-phase extraction cleanup step. Method performance features were successful evaluated in the different seafood samples – algae, mussel, lean and fatty fish muscles, following the criteria established by SANTE/12682/2019 for analytical methods for pesticide residues analysis. The detection and quantification of bisphenol F, musk fragrances (galaxolide and tonalide), UV-filters (2-ethylhexyl salicylate, 2-ethylhexyl 4-methoxycinnamate, and isoamyl 4-methoxycinnamate), and residues of permethrin in commercial samples of algae, mussel and fish collected in a Portuguese estuary support the suitability of the proposed method for future seafood monitoring by food safety authorities.

Content of Carotenoids, Violaxanthin and Neoxanthin in Leaves of Triticum aestivum Exposed to Persistent Environmental Pollutants.

Persistent pollutants such as pharmaceuticals, pesticides, musk fragrances, and dyes are frequently detected in different environmental compartments and negatively impact the environment and humans. Understanding the impacts of diffuse environmental pollutants on plants is still limited, especially at realistic environmental concentrations of contaminants. We studied the effects of key representatives of two major classes of environmental pollutants (nine different antibiotics and six different textile dyes) on the leaf carotenoid (violaxanthin and neoxanthin) content in wheat (Triticum aestivum L.) using different pollutant concentrations and application times. The wheat plants were watered with solutions of selected environmental pollutants in two different concentrations of 0.5 mg L−1 and 1.5 mg L−1 for one week (0.5 L) and two weeks (1 L). Both categories of pollutants selected for this study negatively influenced the content of violaxanthin and neoxanthin, whereas the textile dyes represented more severe stress to the wheat plants. The results demonstrate that chronic exposure to common diffusively spread environmental contaminants constitutes significant stress to the plants.

Organic pollutants in protected plain areas: The occurrence of PAHs, musks, UV-filters, flame retardants and hydrocarbons in woodland soils

Protected woodlands are rare and small portions of the plain territory of northern Italy, where agricultural, industrial and urban activities strongly dominate the landscape. Such natural areas are frequently set on river floodplains and are therefore potentially conditioned by the contamination brought by the surface waters. We investigated the occurrence of multiple categories of organic pollutants, including Polycyclic Aromatic Hydrocarbons (PAHs), Musk fragrances, UV-filters, organophosphorus and novel brominated Flame Retardants (FRs) and Total Petroleum Hydrocarbons (TPH) in woodland soils of eight different protected areas. The samples collected in the floodplains of the Po, Adige and Fratta rivers resulted more contaminated, with levels of PAHs up to 633 ng g−1. Moreover, these samples for the first time revealed the presence of personal care products, primarily 2-ethylhexyl-4-methoxycinnamate (EHMC) and tonalide (AHTN), in soils of protected woodlands, reaching respectively 3.4 ng g−1 and 5.0 ng g−1, together with the occurrence of both organophosphorus and brominated FRs, with total concentrations up to 15 ng g−1. Higher concentrations of hydrocarbons, with TPH in the range 5–65 μg g−1, were instead reflecting the inputs of long chain n-alkanes from epicuticular waxes more than petrogenic contamination.

Effects of the Fragrance Galaxolide on the Biomarker Responses of the Clam Ruditapes philippinarum

The musk fragrance Galaxolide® (HHCB) is widely used in personal care and household products. Its large use leads to a continuous release of the compound into aquatic environments. Although some studies on the presence of HHCB in ecosystems and biota have been conducted, limited data about its effects on organism biomarkers are available. This study aimed at investigating both cellular and biochemical effects of HHCB in the clam Ruditapes philippinarum. Mussels were exposed for 7, 14 and 21 days to 100 ng/L and 500 ng/L of HHCB in seawater, and the effects on haemocyte parameters and antioxidant enzyme activities in the gills and digestive gland were evaluated. In addition, the neurotoxic potential of HHCB and its capacity to cause oxidative damage to proteins were assessed. Overall, our results demonstrated that exposure to HHCB was able to induce changes in biomarker responses of mussels, mainly at the cellular level.

Headspace solid-phase microextraction based on the metal-organic framework CIM-80(Al) coating to determine volatile methylsiloxanes and musk fragrances in water samples using gas chromatography and mass spectrometry

A headspace solid-phase microextraction (HS-SPME) method was developed using the metal-organic framework (MOF) CIM-80(Al) as extraction phase and in combination with gas chromatography-mass spectrometry (GC-MS) for the simultaneous determination of 6 methylsiloxanes and 7 musk fragrances in different environmental waters. The chromatographic separation was optimized in different GC instruments equipped with different detectors, allowing the correct separation and identification of the compounds. The HS-SPME method was optimized using a Box-Behnken experimental design, while the validation was carried out together with the most suitable commercial fiber (divinylbenzene/polydimethylsiloxane) for comparison purposes. The MOF-based coating was particularly efficient for the determination of volatile methylsiloxanes, showing moderately lower limits of detection (of 0.2 and 0.5 μg L−1versus 0.6 μg L−1 for cyclic methylsiloxanes) and slightly better precision (relative standard deviation values lower than 17% versus 22%) than the commercial coating, while avoiding the cross-contamination issues associated to the polymeric composition of commercial fibers. The method was applied for the analysis of seawater and wastewater samples, allowing the quantification of several analytes and the assessment of matrix effects. The proposed HS-SPME method using the CIM-80(Al) fiber constitutes a more environmentally friendly, simpler, and efficient strategy in comparison with other sample preparation methods using different extraction techniques, while the use of a MOF as fiber sorbent constitutes a potential alternative to exploit the features of SPME for the challenging environmental monitoring of these compounds.

Heavy Metal and characterization Study on Momordica Charantia and Hibiscus Rosa-Sinensis Extraction Formulation Herbs Shampoo

The entire world is currently endeavouring to return towards the natural organic materials through its side effects towards ailments. The most popular form of remedy to resolve the scalp issue, boost hair growth, and clean the hair would be through shampoo usage. However, many selected ingredients used in the shampoos sold in the market, such as formaldehyde, halogenated organic compounds, heavy metal, musk fragrances, and coarse coal tar, put many consumers at risk. This paper aims to analyze the heavy metal and characterizations of herbs shampoo containing main organic ingredients are known as Momordica charantia extract, which is rich in vitamin C and amino acids. Besides that, incorporating Hibiscus Rosa-Sinensis as one of the organic ingredients displays amazing results as a booster for hair growth. It can be observed that it helps nourish the development of collagen as it is rich in vitamin C. Meanwhile, the amino acid found could play a vital role in growing healthy locks of hair, nourishment, and as a medium to strengthen the roots. This shampoo has been formalized based on literature findings on its bioactivities and tailored to all the possible variations associated with hair quality, age, frequency of shampooing, and specific problems relating to the scalp's superficial condition. The pH is currently natural with 7.0 with viscosity 1190 cP and density 1.49 g/cm3. The heavy metal study was conducted using inductively coupled plasma (ICP-OES), and it depicts arsenic, cadmium, plumbum, nickel, chromium is not detected (

Polycyclic musk fragrance (PMF) removal, adsorption and biodegradation in a conventional activated sludge wastewater treatment plant in Northern Italy

The first Italian annual monitoring study was carried out in Northern Italy to analyse the fate and removal of polycyclic musk fragrances (PMFs) in a wastewater treatment plant (WWTP) with conventional activated sludge (CAS) system. Water was sampled in four different stations along wastewater treatments to better understand the behaviour of PMFs along different steps of the plant. Galaxolide (HHCB) and galaxolidone (HHCB-lactone) were found in concentrations at μg L-1 level, 1 order of magnitude greater than tonalide (AHTN), whilst phantolide (AHDI) was never detected and celestolide (ADBI) was measured only at trace levels. Considering water concentrations, HHCB and AHTN evidenced a slight reduction, 20% and 50%, respectively, during wastewater treatments, thus resulting in a modest removal efficiency, mainly due to adsorption processes during the biological treatment. This was also confirmed by the high PMF concentrations measured in activated sludges which remained stable throughout the year. On the contrary, HHCB-lactone registered an increase up to 70% during wastewater treatments caused by the biotransformation of the parental compound HHCB during the biological treatment, as shown by the different HHCB-lactone/HHCB ratio measured before and after this step. No significant differences were recorded between seasons in terms of PMF input onto WWTP, in accordance with the common use of these chemicals in civil houses. Overall, this study suggests that current technologies employed in conventional WWTP are not enough efficient in removing these organic micropollutants from wastewaters and, therefore, WWTP effluents represent possible point sources of pollution for aquatic ecosystems. Additional treatments are necessary to enhance the removal of PMFs in order to increase the quality of the WWTP effluents.

Occurrence and Sources of Synthetic Musk Fragrances in the Sewage Treatment Plants and the Han River, Korea

Levels of synthetic musk fragrances (SMFs) and various personal care products (PCPs) were measured in the Han River and its tributaries in Seoul, Korea. The most abundant SMF in all river and PCP samples was 4,6,6,7,8,8-hexamethyl-1,3,4,7-tetrahydrocyclopenta(g)sochromene (HHCB), followed by 1-(3,5,5,6,8,8-hexamethyl-6,7-dihydronaphthalen-2-yl)ethanone (AHTN), musk ketone (MK), and 1,1,2,3,3-pentamethyl-2,5,6,7-tetrahydroinden-4-one (DPMI). The most abundant SMF in both PCPs and the Han River samples was HHCB, followed by AHTN. Moving from upstream to downstream in the Han River, the median SMF concentration was 6.756, 2.945, 0.304, and 0.141 μg/L in the sewage treatment plant (STP) influents, effluents, tributaries, and mainstream, respectively, implying that effective SMF removal was achieved during the sewage treatment process, followed by dilution in the receiving water. Four STPs using advanced biological treatment processes had removal efficiencies of 58.5%, 56.8%, and 38.1% for HHCB, AHTN, and MK, respectively. The highest SMF concentrations in the tributaries were observed at locations close to the STPs. Our study confirmed that the main source of SMFs in the receiving water were sewage effluents containing untreated SMFs, which largely originate from household PCPs, especially hair care products (e.g., shampoo) and perfumes.