Multiple Hot Spots Research Articles

Mirror-enhanced directional out-coupling of SERS by remote excitation of a nanowire-nanoparticle cavity

We report on the experimental observation of mirror-enhanced directional surface-enhanced Raman scattering (SERS) from a self-assembled monolayer of molecules coupled to a nanowire-nanoparticle (NW-NP) junction on a mirror in a remote excitation configuration. Placing the NW-NP junction on a metallic mirror generates multiple gap plasmon modes that have unique momentum space-scattering signatures. We perform Fourier plane imaging of the SERS from the NW-NP on a mirror to understand the effect of multiple hotspots on molecular emission. We systematically study the effect of the ground plane on the directionality of emission from the NW-NP junction and show that the presence of a mirror drastically reduces the angular spread of emission. The effect of multiple hotspots in the geometry on the directionality of the molecular emission is studied using 3D numerical simulations. The results presented here will have implications in understanding plasmon hybridization in the momentum space and its effects on molecular emission.

Detecting the Complex Relationships and Driving Mechanisms of Key Ecosystem Services in the Central Urban Area Chongqing Municipality, China

Ecosystem services (ESs) are highly vulnerable to human activities. Understanding the relationships among multiple ESs and driving mechanisms are crucial for multi-objective management in complex social-ecological systems. The goals of this study are to quantitatively evaluate and identify ESs hotspots, explore the relationships among ESs and elucidate the driving mechanisms. Taking central urban area Chongqing municipality as the study area, biodiversity (BI), carbon fixation (CF), soil conservation (SC) and water conservation (WC) were evaluated based on the InVEST model and ESs hotspots were identified. The complex interactions among multiple ESs were determined by utilizing multiple methods: spearman correlation analysis, bivariate local spatial autocorrelation and K-means clustering. The linear or nonlinear relationships between ESs and drivers were discussed by generalized additive models (GAMs). The results showed that during 2000–2018, except for CF that exhibited no obvious change, all other ESs showed a decrease tendency. High ESs were clustered in mountains, while ESs in urban areas were lowest. At administrative districts scale, ESs were relatively higher in Beibei, Banan and Yubei, and drastically decreased in Jiangbei. Multiple ES hotspots demonstrated clear spatial heterogeneity, which were mainly composed of forestland and distributed in mountainous areas with high altitude and steep slope. The relationships between ES pairs were synergistic at the entire scale. However, at grid scale, the synergies were mainly concentrated in the high-high and low-low clusters, i.e., mountainous areas and urban central areas. Five ESs bundles presented the interactions among multiple ESs, which showed well correspondence with social-ecological conditions. GAMs indicated that forestland and grassland had positive impact on BI and CF. Additionally, SC was mainly determined by geomorphological factors, while WC were mainly influenced by precipitation. Furthermore, policy factors were confirmed to have a certain positive effect on ESs. This study provides credible references for ecosystem management and urban planning.

Numerical study on auto-ignition development and knocking characteristics of a downsized rotary engine under different inlet pressures

In the study, a three-dimensional CFD simulating model coupling with reasonable turbulent model and reduced chemical kinetic mechanism was established and validated. The auto-ignition development and knocking characteristics of a downsized spark-ignition (SI) gasoline rotary engine (RE) under different boosted conditions were numerically investigated. Results showed that as inlet pressure increased from 1.12 bar to 1.16 bar, the knocking intensity (KI) of the RE was enhanced gradually, and the knocking onset was advanced. However, with the further augment of inlet pressure, the KI did not further increase due to the larger heat dissipation loss caused by high turbulent kinetic energy in the long and narrow combustion chamber of the RE. This indicated that the structure of the downsized SI RE had a certain ability of knocking suppression when inlet pressure was sufficiently boosted. The KI of the RE was more serious in the trailing part of the combustion chamber as compared to other positions due to the unidirectional flow field, especially on both sides near the end cover in the trailing part of the combustion chamber. Therefore, it was concluded that strengthening the cooling of both sides near the end cover in the trailing part of the combustion chamber may be an effective way to reduce the KI of a downsized boosted SI RE. In addition, the KI is closely related to auto-ignition development processes, e.g. end-gas auto-ignition modes. Under the mass fraction of unburned mixtures at the moment of end-gas auto-ignition, the local KI caused directly by single hot-spot auto-ignition was higher than that caused by multiple hot-spots auto-ignitions, and the local KI caused by homogeneous auto-ignition was higher than that caused by multiple hot-spots auto-ignitions.

Distribution and protection of avian specialization in Europe

AbstractAimWe assessed the spatial distribution of four different types of avian specialization throughout Europe, identifying landscape features associated with specialization and quantifying where the Natura 2000 network intersects with areas of high avian specialization.LocationEurope.Time periodPresent day.Taxa studiedEuropean breeding birds.MethodsWe used the European Atlas of breeding birds and four avian specialization measures (diet, foraging behaviour, foraging substrate and habitat). We calculated specialization richness and identified geographical hotspots for each of these ecological traits. We tested whether elevational gradient, landscape heterogeneity or dominant land use predicted each type of specialization richness. We determined which types of European protected areas are most associated with higher specialization richness.ResultsDiet and foraging substrate specialists increased with elevation, whereas richness of foraging behavioural specialists decreased. There was a greater richness of dietary and habitat specialists in forests than in other environments. The Natura 2000 areas declared under Bird and both directives (Birds and Habitats) intersected with a high dietary, foraging substrate and habitat specialist richness. The richness of foraging behaviour specialists was high in Birds directive areas. Single and multiple hotspots of specialization were greater in protected than non‐protected areas. However, almost 30% of specialization hotspots did not intersect with protected areas.Main conclusionsOur findings suggest that higher levels of avian specialization in Europe are positively associated with elevation and forested land cover. Importantly, we found that the Natura 2000 network supports all types of avian ecological specialization, albeit mainly in areas declared under both directives.

Occurrence and transport of perfluoroalkyl acids (PFAAs) in a Yangtze River water diversion project during water diversion and flooding

Water diversion is increasingly utilized for water supply, flood control, irrigation, and water quality improvement in many water bodies globally. Our findings indicate that micropollutants such as perfluoroalkyl acids (PFAAs) can be transported via dual-source water diversion projects during water diversion and flood discharge, which may negatively impact both receiving water bodies. However, the sources, spatiotemporal variations, and fluxes of PFAAs under water diversion projects remain unclear. Here we report patterns of 18 PFAA compounds in the surface water at 14 sites of a dual-source water diversion project (Wangyu River) connecting the Yangtze River and Taihu Lake in China. We found multiple contamination hotspots with PFHxA and PFOA dominantly originating from industrial and domestic sources during water diversion from the Yangtze River to Taihu Lake. During the severe flooding in summer 2020, PFAA concentrations ranged from 82.0 to 114.0 ng L−1, while the concentrations and relative contributions of individual perfluoroalkane sulfonic acids (PFSAs) increased along the mainstream due to the tributaries’ contribution and high contamination level in the lake. Based on the spatiotemporal patterns of PFAA pollution, the flux of total PFAAs including both dissolved-phase and SS-phase into Taihu Lake was estimated as 26.6 kg in January 2020 as a reference value for water diversion. To our knowledge, this is the first report to describe the sources, occurrences, and transport of PFAAs in a dual-source water diversion project during water diversion and flooding. The results provide a novel perspective regarding the ecological safety of dual-source water diversion projects.

Importance of human capital, field knowledge and experience to improve pest locust management.

A poorly organised risk management system may dysfunction when used. The consequences can be dramatic for those supposed to be protected. Since the 1960s, preventive control strategies, with field officers as living memory, have been developed to monitor locusts. Preserving their experience of past plagues is consequently essential. Wrong use of their knowledge can disrupt the whole management chain. We explored these conditions using a multi-agent model representing a preventive system. We simulated how the field teams' tendency to repeatedly visit past outbreak areas (hotspots) by allocating them an attraction weight can help in preventing plagues. When field teams' attention remained constant over time, there was dramatic decrease in the number of plagues, with increasing interest in hotspots, as long as interest was less than 2.5 times more than elsewhere. When the field teams were only attentive during recession times, plagues were better controlled using a low weight for hotspots. The spatial structure of hotspot distribution had an effect: the more frequent and the bigger the hotspots, the lower the optimal hotspot weighting needed to reduce plagues. Orienting surveys towards hotspots particularly during recession times reduces plagues. The spatial structure of locust habitats may influence the way they are managed. Habitats located outside the multiple hotspots of species such as the desert locust should be visited more frequently than those with only one hotspot, such as the South American locust. The decline/loss of the field officers' experience highlights the need to save, capitalise and disseminate this knowledge. © 2021 Society of Chemical Industry.

QTL mapping for starch paste viscosity of rice (Oryza sativa L.) using chromosome segment substitution lines derived from two sequenced cultivars with the same Wx allele

BackgroundThe eating and cooking qualities (ECQs) of rice (Oryza sativa L.) are key characteristics affecting variety adoption and market value. Starch viscosity profiles tested by a rapid visco analyzer (RVA) offer a direct measure of ECQs and represent the changes in viscosity associated with starch gelatinization. RVA profiles of rice are controlled by a complex genetic system and are also affected by the environment. Although Waxy (Wx) is the major gene controlling amylose content (AC) and ECQs, there are still other unknown genetic factors that affect ECQs.ResultsQuantitative trait loci (QTLs) for starch paste viscosity in rice were analyzed using chromosome segment substitution lines (CSSLs) developed from the two cultivars 9311 and Nipponbare, which have same Wx-b allele. Thus, the effect of the major locus Wx was eliminated and the other locus associated with the RVA profile could be identified. QTLs for seven parameters of the starch RVA profile were tested over four years in Nanjing, China. A total of 310 QTLs were identified (from 1 to 55 QTLs per trait) and 136 QTLs were identified in more than one year. Among them, 6 QTLs were stalely detected in four years and 26 QTLs were detected in at least three years including 13 pleiotropic loci, controlling 2 to 6 RVA properties simultaneously. These stable QTL hotspots were co-located with several known starch synthesis-related genes (SSRGs). Sequence alignments showed that nucleotide and amino acid sequences of most SSRGs were different between the two parents. Finally, we detected stable QTLs associated with multiple starch viscosity traits near Wx itself, supporting the notion that additional QTLs near Wx control multiple characteristic values of starch viscosity.ConclusionsBy eliminating the contribution from the major locus Wx, multiple QTLs associated with the RVA profile of rice were identified, several of which were stably detected over four years. The complexity of the genetic basis of rice starch viscosity traits might be due to their pleiotropic effects and the multiple QTL hot spots. Minor QTLs controlling starch viscosity traits were identified by using the chromosome segment substitution strategy. Allele polymorphism might be the reason that QTLs controlling RVA profile characteristics were detected in some known SSRG regions.

Self-Constructed Multiple Plasmonic Hotspots on an Individual Fractal to Amplify Broadband Hot Electron Generation.

Plasmonic nanoparticles are ideal candidates for hot-electron-assisted applications, but their narrow resonance region and limited hotspot number hindered the energy utilization of broadband solar energy. Inspired by tree branches, we designed and chemically synthesized silver fractals, which enable self-constructed hotspots and multiple plasmonic resonances, extending the broadband generation of hot electrons for better matching with the solar radiation spectrum. We directly revealed the plasmonic origin, the spatial distribution, and the decay dynamics of hot electrons on the single-particle level by using ab initio simulation, dark-field spectroscopy, pump-probe measurements, and electron energy loss spectroscopy. Our results show that fractals with acute tips and narrow gaps can support broadband resonances (400-1100 nm) and a large number of randomly distributed hotspots, which can provide unpolarized enhanced near field and promote hot electron generation. As a proof-of-concept, hot-electron-triggered dimerization of p-nitropthiophenol and hydrogen production are investigated under various irradiations, and the promoted hot electron generation on fractals was confirmed with significantly improved efficiency.

Plasmon-Coupled Directional Emission from Soluplus-Mediated AgAu Nanoparticles for Attomolar Sensing Using a Smartphone

Although there are numerous techniques for the synthesis of plasmonic (metallic Ag and Au) nanomaterials, eco-friendly and biocompatible methodologies that present diverse applications in nanophotonics and biomedical domains are seldom reported. Soluplus is a graft copolymer of polyvinyl caprolactam–polyvinyl acetate–polyethylene glycol and is extensively used for improving the solubility and bioavailability of poorly water-soluble drugs. However, the utility of their amphiphilic chemical structure, strong UV-light absorbing, and bifunctional properties of the polymer matrix as well as its role as a solubilizer have not been explored for rapid synthesis of plasmonic bimetallic nanohybrids. Although a variety of nanoparticles (NPs) have been studied for surface plasmon-coupled emission (SPCE) spectroscopic applications, AgAu nanohybrids have not been examined in the subject platform hitherto. In this article, we demonstrate a synthesis route for obtaining AgNPs, AuNPs, and AgAu nanohybrids using soluplus as both the reducing and capping agent in a simple UV-light induced one-pot rapid technique. These hybrid nanomaterials were interfaced with propagating surface plasmon polaritons from a 50 nm Ag thin film to understand the plasmonic coupling phenomenon in spacer, cavity, and extended cavity nanoconfigurations. The obtained AgAu nanohybrids aided in addressing the three major long-standing challenges in SPCE technology development, namely, (i) unavoidable quenching in the presence of AuNPs, (ii) chemical instability in AgNPs, and (iii) intrinsic ohmic losses in plasmonic NPs. In addition to overcoming the above-mentioned caveats, unaccustomed >1200-fold sharply directional and polarized SPCE enhancements were achieved using AgAu nanohybrids. The multifold nanogaps generated in AgAu nanohybrid sustained multiple hotspots catering to attomolar sensitivity of the SPCE reporter molecule, rhodamine B (RhB). The proposed methodology to obtain dequenched as well as augmented SPCE enhancements is of utmost utility in biophysicochemical sensor development.

Taxonomic categories for commissioning and startup hot spot strategies

Purpose The purpose of this study is to better understand distinct solution strategy types for common commissioning and startup problems (Hot Spots) in the construction of industrial facilities. The authors also sought to better understand which solution strategy types offer the best value for the effort required. Design/methodology/approach The authors used a method of qualitative coding of text-based data to identify themes, patterns and trends from a collection of 178 commissioning and startup (CSU) solution strategies for the CSU Hot Spots. Coding categories emerged after multiple iterations. The authors also mapped high-value, low-effort solution strategies across the categories. Chi-squared testing and analysis of proportion statistics help quantitatively justify this qualitative research. Findings The authors identified 12 distinct coding categories and showed that they follow a non-uniform distribution via statistical testing. Those strategy types which provide particularly good value for the effort required are identified (such as quality assurance and control strategies), as well as those strategy types that do not. Research limitations/implications Research is limited to CSU for the construction of industrial facilities. The findings are also limited to a subset of the most commonly problematic CSU activities. Many findings may be beneficial for heavy civil and commercial CSU as well. Practical implications Coding categories, definitions and descriptions provide a good overview of all 178 solution strategies for CSU project professionals. Implementing certain types of solutions or solution programs may allow CSU managers to prevent multiple Hot Spots from becoming problematic or to keep problems already occurring from becoming worse. Managers will also benefit from knowledge about which strategy types are more likely to give a higher value for lower effort. Originality/value Qualitative coding and analysis of solution strategies for common CSU problems have never been conducted so rigorously in any other CSU study. This method has yielded results distinct from other CSU studies which have used quantitative methods. Insights from findings have advanced the body of knowledge surrounding problem-solving in the commissioning and startup sub-discipline.

Improvement of hotspot cooling in confinement space by corona discharge induced ionic wind

Hotspot cooling is a significant problem in the thermal management of electronic devices. With device dimension scaling down, the dissipation space becomes more and more confined making hotspot cooling challenged. However, effective solutions are limited. In this study, the feasibility of hotspot cooling by ionic wind in confined space is justified. It is demonstrated that the hotspot in a confined channel can be suppressed effectively by ionic wind, which cannot be achieved by conventional pressure-driven flow even with a large Reynolds number. The underlying physics is revealed by the secondary vortices. The cooling strategies for a single hotspot and multiple hotspots are proposed. There exists strong interplay between the secondary corona jet and the bulk flow. Coupled mechanisms (impingement effect, barrier effect, and entrance effect) are explained to design a suitable electrode configuration for single hotspot cooling. However, the design strategy for single hotspot cannot be directly used for multiple hotspots and a new design concept of “intensify electrode pair” is proposed. It is also found that partial ground configuration is suitable for a single hotspot, while entire ground is better for multiple hotspots. This work provides a potential means for hotspot cooling of electronic devices in confined space.

Urban hotspots detection of taxi stops with local maximum density

When getting on and off the taxis, people prefer to choose around landmarks, such as a shopping mall, the gate of a residential unit, or a road intersection. This preference leads to multiple local peaks in spatial density, existing in both highly popular and less popular regions. These multiple local peaks provide a natural form to describe small-scale hotspots where the pick-up and drop-off events actually happen. However, they have not been fully studied. In this paper, we propose the local maximum density (LMD) approach to identify the hotspots as a small area around a local maximum density of incidents. It is evaluated using a 6-month taxi dataset of Wuhan City, where 90 m × 90 m square is recommended as the size of a hotspot by LMD. Results show that LMD not only identifies multiple local hotspots in highly popular regions, but also detects potential hotspots in less popular regions. Moreover, a non-uniform spatial pattern is found between pick-up and drop-off local hotspots. Comparing with pick-up behaviors, drop-off behaviors diffuse more in less popular hotspots, but also concentrate more on highly popular hotspots. The driving factors of this phenomenon are further explored by analyzing the match mode of pick-up and drop-off local hotspots with different popularity.

Chromosomal characteristics of salt stress heritable gene expression in the rice genome

BackgroundGene expression is potentially an important heritable quantitative trait that mediates between genetic variation and higher-level complex phenotypes through time and condition-dependent regulatory interactions. Therefore, we sought to explore both the genomic and condition-specific characteristics of gene expression heritability within the context of chromosomal structure.ResultsHeritability was estimated for biological gene expression using a diverse, 84-line, Oryza sativa (rice) population under optimal and salt-stressed conditions. Overall, 5936 genes were found to have heritable expression regardless of condition and 1377 genes were found to have heritable expression only during salt stress. These genes with salt-specific heritable expression are enriched for functional terms associated with response to stimulus and transcription factor activity. Additionally, we discovered that highly and lowly expressed genes, and genes with heritable expression are distributed differently along the chromosomes in patterns that follow previously identified high-throughput chromosomal conformation capture (Hi-C) A/B chromatin compartments. Furthermore, multiple genomic hot-spots enriched for genes with salt-specific heritability were identified on chromosomes 1, 4, 6, and 8. These hotspots were found to contain genes functionally enriched for transcriptional regulation and overlaps with a previously identified major QTL for salt-tolerance in rice.ConclusionsInvestigating the heritability of traits, and in-particular gene expression traits, is important towards developing a basic understanding of how regulatory networks behave across a population. This work provides insights into spatial patterns of heritable gene expression at the chromosomal level.

Chromosome-level genome assembly and manually-curated proteome of model necrotroph Parastagonospora nodorum Sn15 reveals a genome-wide trove of candidate effector homologs, and redundancy of virulence-related functions within an accessory chromosome

BackgroundThe fungus Parastagonospora nodorum causes septoria nodorum blotch (SNB) of wheat (Triticum aestivum) and is a model species for necrotrophic plant pathogens. The genome assembly of reference isolate Sn15 was first reported in 2007. P. nodorum infection is promoted by its production of proteinaceous necrotrophic effectors, three of which are characterised – ToxA, Tox1 and Tox3.ResultsA chromosome-scale genome assembly of P. nodorum Australian reference isolate Sn15, which combined long read sequencing, optical mapping and manual curation, produced 23 chromosomes with 21 chromosomes possessing both telomeres. New transcriptome data were combined with fungal-specific gene prediction techniques and manual curation to produce a high-quality predicted gene annotation dataset, which comprises 13,869 high confidence genes, and an additional 2534 lower confidence genes retained to assist pathogenicity effector discovery. Comparison to a panel of 31 internationally-sourced isolates identified multiple hotspots within the Sn15 genome for mutation or presence-absence variation, which was used to enhance subsequent effector prediction. Effector prediction resulted in 257 candidates, of which 98 higher-ranked candidates were selected for in-depth analysis and revealed a wealth of functions related to pathogenicity. Additionally, 11 out of the 98 candidates also exhibited orthology conservation patterns that suggested lateral gene transfer with other cereal-pathogenic fungal species. Analysis of the pan-genome indicated the smallest chromosome of 0.4 Mbp length to be an accessory chromosome (AC23). AC23 was notably absent from an avirulent isolate and is predominated by mutation hotspots with an increase in non-synonymous mutations relative to other chromosomes. Surprisingly, AC23 was deficient in effector candidates, but contained several predicted genes with redundant pathogenicity-related functions.ConclusionsWe present an updated series of genomic resources for P. nodorum Sn15 – an important reference isolate and model necrotroph – with a comprehensive survey of its predicted pathogenicity content.

Petrogenesis of Basaltic Lavas From the West Pacific Seamount Province: Geochemical and Sr‐Nd‐Pb‐Hf Isotopic Constraints

AbstractIn this study, we report new major and trace element, Sr‐Nd‐Pb‐Hf isotopic compositions and Ar‐Ar ages for basaltic lavas from seven poorly studied seamounts/guyots in the Western Pacific Seamount Province (WPSP). A review of new and published Ar‐Ar ages for seamounts/guyots confirm no linear age progression, as would be expected if these seamounts were produced by the Pacific Plate moving over a stationary mantle plume. These samples exhibit an alkalic character and can be produced by small‐degree partial melting of garnet pyroxenite with CO2. Their isotopic data identify four mantle end‐members (DMM, EMI, EMII, and HIMU) in the WPSP source region. These results confirm previous studies that invoke multiple contemporaneous plumelets in the region and a heterogeneous upper mantle reservoir which has been overprinted by multiple discrete hotspots, which create an anomalous and heterogeneous upper mantle because of mixing/overlapping EMI, EMII, and HIMU tracks, and age progressive volcanism is totally obscured. We propose a model where relict fragments of enriched mantle with distinct isotopic compositions residing in the upper mantle were a product of the formation of the adjacent large oceanic plateaus. These events introduce heterogeneity in the depleted mantle. The infiltration and metasomatism of these relict enriched components by carbonatitic fluids from the low‐velocity zone, create a source that then underwent small degrees of decompressional melting. The partial melts exploited pre‐existing lithospheric faults as an upwelling conduit and the resultant seafloor lava flows have obscured evidence to those pre‐existing lithospheric faults.

Optimization-free customization of optical tightly focused fields: uniform needles and hotspot chains.

An optimization-free method based on an inverse problem of nonlinear equations is employed to design the binary phase diffraction optical element (BPDOE) that could modulate the incident light of a high-numerical-aperture (NA) objective lens so that the axisymmetric focal fields can be customized on demand. For example, a 43λ-long optical longitudinally polarized needle with its lateral size beyond diffraction limit is reported by using a 27-belt BPDOE, where the cost evaluated by the ratio of the belt number of BPDOE to the length of needle is record small compared with other optimization algorithms. Moreover, another longitudinal field with multiple hotspots along the propagation direction of light is also achieved with a 10-belt BPDOE. These achieved focal fields are verified doubly by using a finite-difference time-domain (FDTD) method, indicating the validity of Richards-Wolf vector diffraction theory. This optimization-free approach makes the design of BPDOEs with numerous belts viable to generate the expected focal fields, which might benefit various applications such as optical trapping, super-resolution imaging, and lithography.

Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe.

Infectious bronchitis of chicken is a high morbidity and mortality viral disease affecting the poultry industry worldwide; therefore, a better understanding of this pathogen is of utmost importance. The primary aim of this study was to obtain a deeper insight into the genomic diversity of field infectious bronchitis virus (IBV) strains using phylogenetic and recombination analysis. We sequenced the genome of 20 randomly selected strains from seven European countries. After sequencing, we created a genome sequence data set that contained 36 European origin field isolates and 33 vaccine strains. When analyzing these 69 IBV genome sequences, we identified 215 recombination events highlighting that some strains had multiple recombination breaking points. Recombination hot spots were identified mostly in the regions coding for non-structural proteins, and multiple recombination hot spots were identified in the nsp2, nsp3, nsp8, and nsp12 coding regions. Recombination occurred among different IBV genotypes and involved both field and vaccine IBV strains. Ninety percent of field strains and nearly half of vaccine strains showed evidence of recombination. Despite the low number and the scattered geographical and temporal origin of whole-genome sequence data collected from European Gammacoronaviruses, this study underlines the importance of recombination as a major evolutionary mechanism of IBVs.

Teknik Pemeriksaan Kedokteran Nuklir Bone Scan di Instalasi Radiologi RSUP Dr. Kariadi Semarang

Background: A bone scan or commonly referred to as bone print is nuclear medicine examination using a radioactive substance or radiopharmaceutical that is inserted into the body through intravenous injection which aims to help diagnose abnormalities that occur in the bone. This imaging procedure uses a radiopharmaceutical 99mTc-MDP (methylenediphosphonate) is the most commonly used radiopharmaceutical.Methods: The patient will be injected with this radiopharmaceutical at a dose of 15-20 mCi, through the vein in the hand. Imaging can be done as soon as the radiopharmaceutical is injected or after a while to wait for the radiopharmaceutical to be distributed and absorbed by the bone, about 3-5 hours later. Imaging is done by three-phase method, namely the first phase (Vascular phase), the second phase (Blood Pool phase), and the third phase (Total body phase) l.Results: The bone scan method is an efficient examination because in 1x the imaging can provide a complete picture from the head to the foot. Evaluation of results, under normal conditions the distribution of radioactivity in the bone appears symmetrical.Conclusion: In the process of bone metastasis, it can be seen that typical pathological radioactivity can be multiple (multiple hot spots). Malignant tumors can be distinguished from benign tumors by blood pool examination.

Bundles and Hotspots of Multiple Ecosystem Services for Optimized Land Management in Kentucky, United States

Ecosystem services are benefits that the natural environment provides to support human well-being. A thorough understanding and assessment of these services are critical to maintain ecosystem services flow through sustainable land management to optimize bundles of ecosystem services provision. Maximizing one particular ecosystem service may lead to reduction in another. Therefore, identifying ecosystem services tradeoffs and synergies is key in addressing this challenge. However, the identification of multiple ecosystem services tradeoffs and synergies is still limited. A previous study failed to effectively capture the spatial interaction among ecosystem services as it was limited by “space-to-time” substitution method used because of temporal data scarcity. The study was also limited by using land use types in creating ecosystem services, which could lead to some deviations. The broad objective of this study is therefore to examine the bundles and hotspots of multiple ecosystem services and their tradeoffs in Kentucky, U.S. The study combined geographic data and spatially-explicit models to identify multiple ecosystem services bundles and hotspots, and determined the spatial locations of ecosystem services hotspots. Results showed that the spatial interactions among ecosystem services were very high: of the 21 possible pairs of ecosystem services, 17 pairs were significantly correlated. The seven ecosystem services examined can be bundled into three groups, geographically clustered on the landscape. These results support the hypothesis that some groups of ecosystem services provision can present similar spatial patterns at a large mesoscale. Understanding the spatial interactions and bundles of the ecosystem services provides essential information for evidence-based sustainable land management.

Genome Plasticity by Insertion Sequences Learned From a Case of Radiation-Resistant Bacterium Deinococcus geothermalis.

The genome of the radiation-resistant bacterium Deinococcus geothermalis contains 19 types of insertion sequences (ISs), including 93 total transposases (Tpases) in 73 full-length ISs from the main chromosome and 2 mega plasmids. In this study, 68 ISs from the D. geothermalis genome were extracted to implicate the earlier genome before its mutation by transposition of ISs. The total size of eliminated ISs from genome was 78.85 kb. From these in silico corrections of mutation by the ISs, we have become aware of some bioinformatics factualness as follows: (1) can reassemble the disrupted genes if the exact IS region was eliminated, (2) can configure the schematic clustering of major DDE type Tpases, (3) can determine IS integration order across multiple hot spots, and (4) can compare genetic relativeness by the partial synteny analysis between D. geothermalis and Deinococcus strain S9. Recently, we found that several IS elements actively transferred to other genomic sites under hydrogen peroxide-induced oxidative stress conditions, resulting in the inactivation of functional genes. Therefore, the single species genome’s mobilome study provides significant support to define bacterial genome plasticity and molecular evolution from past and present progressive transposition events.